AlfalfaGEDB | MS.gene84998

M. sativa cultivar XinJiangDaYe / MS.gene84998

KEGG: K17893
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009916 GO:0016491 GO:0016679 GO:0016682 GO:0055114
PFAM: PF01786.20

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84998.t1	AGQ42774.1	99.7	336	0	1	1	336	1	335	1.20E-195	692.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84998.t1	Q39219	79.7	311	60	1	29	336	44	354	2.4e-144	513.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84998.t1	S5FNL5	99.7	336	0	1	1	336	1	335	8.6e-196	692.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060237	MS.gene84998	0.814998	1.16E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene028441	MS.gene84998	PPI
MS.gene35020	MS.gene84998	PPI
MS.gene84998	MS.gene70368	PPI
MS.gene90178	MS.gene84998	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84998.t1	MTR_5g026620	95.238	336	10	3	1	336	1	330	0.0	663
MS.gene84998.t1	MTR_5g026620	98.667	225	3	0	112	336	38	262	5.12e-169	471
MS.gene84998.t1	MTR_4g094222	75.600	250	61	0	87	336	96	345	1.94e-149	424
MS.gene84998.t1	MTR_5g070680	67.424	264	86	0	73	336	60	323	1.40e-139	398
MS.gene84998.t1	MTR_5g070870	66.667	273	88	1	67	336	52	324	2.73e-133	382

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene84998.t1	AT3G22370	79.743	311	60	1	29	336	44	354	0.0	521
MS.gene84998.t1	AT3G22360	84.249	273	41	1	66	336	53	325	8.79e-176	490
MS.gene84998.t1	AT3G27620	82.528	269	39	1	76	336	61	329	8.15e-169	473
MS.gene84998.t1	AT1G32350	70.545	275	73	1	70	336	44	318	8.64e-154	434
MS.gene84998.t1	AT5G64210	68.293	287	80	3	52	336	76	353	1.09e-147	420
MS.gene84998.t1	AT1G32350	70.703	256	67	1	70	317	44	299	1.10e-142	405

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Find 87 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGGATTACGAGTTATT+GGG	0.318703	5.2:-17635301	MS.gene84998:CDS
AAAGGGATTACGAGTTATTG+GGG	0.549336	5.2:-17635300	MS.gene84998:CDS
AAATTTGCACACCGTATGGT+TGG	0.563076	5.2:-17633690	MS.gene84998:CDS
AACCATACGGTGTGCAAATT+TGG	0.112313	5.2:+17633692	None:intergenic
AAGAAGATGATGATGAGGCA+TGG	0.686981	5.2:-17635555	None:intergenic
AAGATGATGATGAGGCATGG+TGG	0.746370	5.2:-17635552	MS.gene84998:CDS
AAGCATTGAAGAAAACACCC+TGG	0.672042	5.2:+17633739	None:intergenic
AAGTTGGTGTTCCAAACAAA+TGG	0.440248	5.2:-17635470	MS.gene84998:CDS
AATGGAAATGGAATTGCTTC+AGG	0.325465	5.2:-17635239	MS.gene84998:CDS
ACAAAATAACCCCATTTGTT+TGG	0.260248	5.2:+17635459	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGGGATTACGAGTTAT+TGG	-	chr5.2:17633395-17633414	MS.gene84998:intron	30.0%
AAAAGGGATTACGAGTTATT+GGG	-	chr5.2:17633396-17633415	MS.gene84998:intron	30.0%
ACAAAATAACCCCATTTGTT+TGG	+	chr5.2:17633241-17633260	None:intergenic	30.0%
ATAGGATAGTAAGTTCATCA+AGG	+	chr5.2:17634626-17634645	None:intergenic	30.0%
CATCGAATCTTTATTAGAAG+AGG	-	chr5.2:17633829-17633848	MS.gene84998:CDS	30.0%
CATTCAAAATACTCAGATGA+AGG	+	chr5.2:17635449-17635468	None:intergenic	30.0%
CTATAAGCTACAAATTCAAG+AGG	-	chr5.2:17635280-17635299	MS.gene84998:CDS	30.0%
CTCTTCTAATAAAGATTCGA+TGG	+	chr5.2:17633831-17633850	None:intergenic	30.0%
CTTCTTTGCAAACAACATTA+CGG	+	chr5.2:17633191-17633210	None:intergenic	30.0%
GGTGATAACAAAGATGAAAA+AGG	-	chr5.2:17633379-17633398	MS.gene84998:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	17633148	17635571	17633148	ID=MS.gene84998
chr5.2	mRNA	17633148	17635571	17633148	ID=MS.gene84998.t1;Parent=MS.gene84998
chr5.2	exon	17635239	17635571	17635239	ID=MS.gene84998.t1.exon1;Parent=MS.gene84998.t1
chr5.2	CDS	17635239	17635571	17635239	ID=cds.MS.gene84998.t1;Parent=MS.gene84998.t1
chr5.2	exon	17635027	17635155	17635027	ID=MS.gene84998.t1.exon2;Parent=MS.gene84998.t1
chr5.2	CDS	17635027	17635155	17635027	ID=cds.MS.gene84998.t1;Parent=MS.gene84998.t1
chr5.2	exon	17633494	17633982	17633494	ID=MS.gene84998.t1.exon3;Parent=MS.gene84998.t1
chr5.2	CDS	17633494	17633982	17633494	ID=cds.MS.gene84998.t1;Parent=MS.gene84998.t1
chr5.2	exon	17633148	17633207	17633148	ID=MS.gene84998.t1.exon4;Parent=MS.gene84998.t1
chr5.2	CDS	17633148	17633207	17633148	ID=cds.MS.gene84998.t1;Parent=MS.gene84998.t1

Gene Sequence

>MS.gene84998

ATGATGATGAGGCATGGTGGTCACGGTGCTGCTATGAACACCGTAATGTTGTTTGCAAAGAAGGGTTTATTAGGTGGTGAAGTTGGTGTTCCAAACAAATGGGGTTATTTTGTTAGATCAACAACCCCTCTTGTAAGGAACGCAAGCACTTTCACTGCTAATCTGTCAGATCAGAAGGACGACAAGACTGTCGACAAAACTCCTCCGTCGTCGTCGTCTCAGGGTGGTGCTGGTGATAACAAAGATGAAAAAGGGATTACGAGTTATTGGGGTGTTCAACCTTCTAAGATCACAAAACCTGATGGTACTGAATGGAAATGGAATTGCTTCAGGGTATGTTATTGCTATGTTACATCTTTTGATCTTGTTTTTTGAGAAAAATGATTTTTTTTTAATGTGGATTTTGATTGTGACAGCCATGGGAGACATACAAAGCAGATGTTACGATTGATCTGACTAAACATCATAAACCAACAACTTTTCTGGACAAGATGGCTTATTGGACTGTTAAGAGTCTTAGGTATCCAACTGATCTCTTTTTTCAGGTAATGTTCTCTTTAATTGATATTGTGAGTGTGATTTTTATTCATTATTATTATTATTATTATTATAAAAATCCGGGTTCTGGATCGATGAGAGTTGGATCTAGCAGAAGGGTTAGACTCCCACAATGTAGCGAACCATCGAATCTTTATTAGAAGAGGTGCTAGCATTACCTTACCTCCTTTGAAAAAAATTAACAAAAAAATGGATTTCAGGAATTACTGGTGTGATTTTGTGAGTTTGTATTTATATATTTTATTTATTTTATGGTCAGGGTTTTATTAATTATATCAGCACTTATTATTTTAAGAGTATATATAAAAAAATGAAATTGTATTTTTTGTCAAAATAGAATGAAATTGTATTTAAAAATTTTAAATCAGTTACACCTAACTCCACCTAACTCCTGAAGAGGTTTTAAGGTACCTTTGTTCAAGGCCGCCATCATATTTTTAATTAGGTTTCTTGTGTGTTTCCTAAATATTCAATCATGCATAATGCATGTGATGTTTGGCTTGTTTGGATGCATGTGCTTTGGTAAGGTCAGTTGGTTACATGAAATGGGAAGTTAAATAATGATCCAATGTTTTATACTGTATGGTAAAAATAAAAATCCACCACAGAATGATGACCTTTTGTTTTGAAATAATCCACCACAAGATGGTGCCTTTTACCTTTTGGTTTGAAAAGAAATCCACTTGCTAGCTATTATTGTTATTTCCTCTAAAAAACAATATAATTGACATGTTAATTAGCACTTAGTCTTAACACTTTTCATTTGAAAATAAAAAATTGCTTAAACTGGTCTTCTGTGAGAAATCATTTCATATGAGAGTTGTAAAGTGTCAGTATTATTTTATATTGAAATATCAATCTATCATCTCCCTAATAAGAATGAAATTTGAATTGAATTTTATAGTAATATGTTCTCCCCTTGATGAACTTACTATCCTATAAATTATGTTCAAAGTTTCTTTTAGTTACTAACACCAAGATCAAAAGTATTTTATGTAATTTCTAACTTAAGTTGTATTTAATTGAAACAGAGAAGGTATGGATGCCGAGCAATGATGCTTGAGACAGTAGCTGCTGTCCCTGGCATGGTAGGAGGAATGCTGCTTCACTGCAAATCATTGCGCCGATTTGAGCACAGTGGTGGATGGATCAAAGCTTTGCTAGAAGAAGCAGAGAACGAGCGCATGCACCTAATGACTTTCATGGAAGTGGCAAAGCCAAAGTGGTATGAGCGTGCACTTGTCATAACTGTCCAGGGTGTTTTCTTCAATGCTTATTTCTTAGGCTATTTGCTCTCACCCAAATTTGCACACCGTATGGTTGGTTACCTTGAGGAAGAAGCTATTCACTCATACACTGAGTTTCTCAAGGAGCTTGACAAGGGTAATATTGAAAATGTCCCTGCTCCAGCTATTGCTATTGACTATTGGCAGCTTCCTCAAAACTCTACTTTAAGGGATGTTGTCGAGGTTGTCAGGGCTGATGAAGCACATCATCGCGATGTTAACCACTTTGCATCGGTATGTTTGTCTATCCCTTTGTCTCACTTTTGCGCAATCTATCCATTTATTTATCTATAAGCTACAAATTCAAGAGGCAGCTAGACGCATGTCCATGATCTGTCTTTTTATTATGATTCAGAGTGTATAAAGTACACATTTCTCAAACTGTGACATAGATAAGTCTTTATACATTCAAAACCATAGTTGTGTCTTTTTAGAAATGGAAAAAATCTTAGATCCTTCATCTGAGTATTTTGAATGGGATTCAAGAACACTGATTGTGTTTTCATGTAATGAATTGCAGGATATACATTACCAAGGACGAGAGTTAAGGGAGGCTGCTGCTCCAATTGGTTATCACTAG

Protein sequence

>MS.gene84998.t1

MMMRHGGHGAAMNTVMLFAKKGLLGGEVGVPNKWGYFVRSTTPLVRNASTFTANLSDQKDDKTVDKTPPSSSSQGGAGDNKDEKGITSYWGVQPSKITKPDGTEWKWNCFRPWETYKADVTIDLTKHHKPTTFLDKMAYWTVKSLRYPTDLFFQRRYGCRAMMLETVAAVPGMVGGMLLHCKSLRRFEHSGGWIKALLEEAENERMHLMTFMEVAKPKWYERALVITVQGVFFNAYFLGYLLSPKFAHRMVGYLEEEAIHSYTEFLKELDKGNIENVPAPAIAIDYWQLPQNSTLRDVVEVVRADEAHHRDVNHFASDIHYQGRELREAAAPIGYH