Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35020.t1 | AGQ42775.1 | 99.7 | 344 | 1 | 0 | 1 | 344 | 1 | 344 | 4.00E-162 | 580.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35020.t1 | Q41266 | 92.7 | 248 | 18 | 0 | 97 | 344 | 86 | 333 | 1.3e-137 | 490.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35020.t1 | S5FDW5 | 99.7 | 344 | 1 | 0 | 1 | 344 | 1 | 344 | 2.9e-162 | 580.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049153 | MS.gene35020 | 0.800658 | 1.30E-48 | -1.69E-46 |
| MS.gene049170 | MS.gene35020 | 0.806192 | 9.27E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene35020 | 0.812464 | 4.18E-51 | -1.69E-46 |
| MS.gene049488 | MS.gene35020 | 0.823204 | 1.57E-53 | -1.69E-46 |
| MS.gene049910 | MS.gene35020 | 0.803768 | 2.98E-49 | -1.69E-46 |
| MS.gene050060 | MS.gene35020 | 0.872934 | 2.17E-67 | -1.69E-46 |
| MS.gene050400 | MS.gene35020 | 0.811482 | 6.85E-51 | -1.69E-46 |
| MS.gene050426 | MS.gene35020 | -0.879144 | 1.58E-69 | -1.69E-46 |
| MS.gene050464 | MS.gene35020 | 0.801191 | 1.01E-48 | -1.69E-46 |
| MS.gene050642 | MS.gene35020 | 0.800068 | 1.71E-48 | -1.69E-46 |
| MS.gene050670 | MS.gene35020 | 0.881892 | 1.65E-70 | -1.69E-46 |
| MS.gene050971 | MS.gene35020 | 0.850514 | 1.62E-60 | -1.69E-46 |
| MS.gene05105 | MS.gene35020 | 0.802091 | 6.61E-49 | -1.69E-46 |
| MS.gene051360 | MS.gene35020 | 0.800397 | 1.47E-48 | -1.69E-46 |
| MS.gene051521 | MS.gene35020 | 0.83 | 3.76E-55 | -1.69E-46 |
| MS.gene051525 | MS.gene35020 | 0.869484 | 2.99E-66 | -1.69E-46 |
| MS.gene051582 | MS.gene35020 | 0.812929 | 3.31E-51 | -1.69E-46 |
| MS.gene051983 | MS.gene35020 | 0.827165 | 1.82E-54 | -1.69E-46 |
| MS.gene052011 | MS.gene35020 | -0.864665 | 1.03E-64 | -1.69E-46 |
| MS.gene052077 | MS.gene35020 | 0.861351 | 1.09E-63 | -1.69E-46 |
| MS.gene052134 | MS.gene35020 | 0.822533 | 2.25E-53 | -1.69E-46 |
| MS.gene052135 | MS.gene35020 | 0.838351 | 3.03E-57 | -1.69E-46 |
| MS.gene052136 | MS.gene35020 | 0.810656 | 1.03E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene35020 | 0.865662 | 5.02E-65 | -1.69E-46 |
| MS.gene052253 | MS.gene35020 | 0.863897 | 1.79E-64 | -1.69E-46 |
| MS.gene052340 | MS.gene35020 | 0.860964 | 1.43E-63 | -1.69E-46 |
| MS.gene052414 | MS.gene35020 | 0.83743 | 5.22E-57 | -1.69E-46 |
| MS.gene052469 | MS.gene35020 | 0.836849 | 7.35E-57 | -1.69E-46 |
| MS.gene052481 | MS.gene35020 | 0.811356 | 7.29E-51 | -1.69E-46 |
| MS.gene052485 | MS.gene35020 | 0.822944 | 1.81E-53 | -1.69E-46 |
| MS.gene053169 | MS.gene35020 | 0.823028 | 1.73E-53 | -1.69E-46 |
| MS.gene05316 | MS.gene35020 | 0.805926 | 1.05E-49 | -1.69E-46 |
| MS.gene053449 | MS.gene35020 | 0.805686 | 1.18E-49 | -1.69E-46 |
| MS.gene054102 | MS.gene35020 | 0.802829 | 4.66E-49 | -1.69E-46 |
| MS.gene054123 | MS.gene35020 | 0.817417 | 3.33E-52 | -1.69E-46 |
| MS.gene054183 | MS.gene35020 | 0.862896 | 3.66E-64 | -1.69E-46 |
| MS.gene054349 | MS.gene35020 | 0.845905 | 3.03E-59 | -1.69E-46 |
| MS.gene054556 | MS.gene35020 | 0.849316 | 3.50E-60 | -1.69E-46 |
| MS.gene054719 | MS.gene35020 | 0.80717 | 5.76E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene35020 | 0.813228 | 2.85E-51 | -1.69E-46 |
| MS.gene054983 | MS.gene35020 | 0.802446 | 5.59E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene35020 | 0.834988 | 2.18E-56 | -1.69E-46 |
| MS.gene055369 | MS.gene35020 | 0.8281 | 1.08E-54 | -1.69E-46 |
| MS.gene055539 | MS.gene35020 | 0.867038 | 1.84E-65 | -1.69E-46 |
| MS.gene05553 | MS.gene35020 | 0.890595 | 8.55E-74 | -1.69E-46 |
| MS.gene05556 | MS.gene35020 | 0.862085 | 6.51E-64 | -1.69E-46 |
| MS.gene055741 | MS.gene35020 | 0.827068 | 1.92E-54 | -1.69E-46 |
| MS.gene055744 | MS.gene35020 | 0.840782 | 7.07E-58 | -1.69E-46 |
| MS.gene056233 | MS.gene35020 | 0.842914 | 1.93E-58 | -1.69E-46 |
| MS.gene056407 | MS.gene35020 | 0.814054 | 1.87E-51 | -1.69E-46 |
| MS.gene056514 | MS.gene35020 | 0.833345 | 5.62E-56 | -1.69E-46 |
| MS.gene056659 | MS.gene35020 | 0.866444 | 2.84E-65 | -1.69E-46 |
| MS.gene056723 | MS.gene35020 | 0.856997 | 2.20E-62 | -1.69E-46 |
| MS.gene056899 | MS.gene35020 | -0.821221 | 4.53E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene35020 | 0.816696 | 4.84E-52 | -1.69E-46 |
| MS.gene057124 | MS.gene35020 | 0.854167 | 1.47E-61 | -1.69E-46 |
| MS.gene057396 | MS.gene35020 | 0.861626 | 8.99E-64 | -1.69E-46 |
| MS.gene057448 | MS.gene35020 | 0.807817 | 4.20E-50 | -1.69E-46 |
| MS.gene057450 | MS.gene35020 | 0.80285 | 4.61E-49 | -1.69E-46 |
| MS.gene057476 | MS.gene35020 | 0.81211 | 5.00E-51 | -1.69E-46 |
| MS.gene057749 | MS.gene35020 | 0.838473 | 2.82E-57 | -1.69E-46 |
| MS.gene057823 | MS.gene35020 | 0.835261 | 1.86E-56 | -1.69E-46 |
| MS.gene057825 | MS.gene35020 | 0.812491 | 4.13E-51 | -1.69E-46 |
| MS.gene057833 | MS.gene35020 | 0.816738 | 4.74E-52 | -1.69E-46 |
| MS.gene058007 | MS.gene35020 | 0.863997 | 1.67E-64 | -1.69E-46 |
| MS.gene058142 | MS.gene35020 | 0.81717 | 3.79E-52 | -1.69E-46 |
| MS.gene058376 | MS.gene35020 | 0.8253 | 5.05E-54 | -1.69E-46 |
| MS.gene058444 | MS.gene35020 | 0.857352 | 1.73E-62 | -1.69E-46 |
| MS.gene058700 | MS.gene35020 | 0.869297 | 3.44E-66 | -1.69E-46 |
| MS.gene058710 | MS.gene35020 | 0.831811 | 1.35E-55 | -1.69E-46 |
| MS.gene05886 | MS.gene35020 | 0.820142 | 8.02E-53 | -1.69E-46 |
| MS.gene058928 | MS.gene35020 | 0.820087 | 8.25E-53 | -1.69E-46 |
| MS.gene058948 | MS.gene35020 | 0.818748 | 1.67E-52 | -1.69E-46 |
| MS.gene058949 | MS.gene35020 | 0.815387 | 9.49E-52 | -1.69E-46 |
| MS.gene059011 | MS.gene35020 | 0.835695 | 1.44E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene35020 | 0.821426 | 4.06E-53 | -1.69E-46 |
| MS.gene059082 | MS.gene35020 | 0.828073 | 1.10E-54 | -1.69E-46 |
| MS.gene059285 | MS.gene35020 | 0.84309 | 1.74E-58 | -1.69E-46 |
| MS.gene059420 | MS.gene35020 | 0.877195 | 7.64E-69 | -1.69E-46 |
| MS.gene059462 | MS.gene35020 | 0.836289 | 1.02E-56 | -1.69E-46 |
| MS.gene059688 | MS.gene35020 | 0.829567 | 4.79E-55 | -1.69E-46 |
| MS.gene059692 | MS.gene35020 | 0.817906 | 2.59E-52 | -1.69E-46 |
| MS.gene059695 | MS.gene35020 | 0.819652 | 1.04E-52 | -1.69E-46 |
| MS.gene059717 | MS.gene35020 | 0.81512 | 1.09E-51 | -1.69E-46 |
| MS.gene059807 | MS.gene35020 | 0.809525 | 1.81E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene35020 | 0.817108 | 3.91E-52 | -1.69E-46 |
| MS.gene059868 | MS.gene35020 | 0.824437 | 8.08E-54 | -1.69E-46 |
| MS.gene059946 | MS.gene35020 | 0.806011 | 1.01E-49 | -1.69E-46 |
| MS.gene060247 | MS.gene35020 | 0.854767 | 9.89E-62 | -1.69E-46 |
| MS.gene060403 | MS.gene35020 | 0.837714 | 4.42E-57 | -1.69E-46 |
| MS.gene060986 | MS.gene35020 | 0.81984 | 9.40E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene35020 | 0.823518 | 1.33E-53 | -1.69E-46 |
| MS.gene061051 | MS.gene35020 | -0.802979 | 4.34E-49 | -1.69E-46 |
| MS.gene061154 | MS.gene35020 | 0.840292 | 9.50E-58 | -1.69E-46 |
| MS.gene061159 | MS.gene35020 | 0.824961 | 6.08E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene35020 | MS.gene028102 | PPI |
| MS.gene72816 | MS.gene35020 | PPI |
| MS.gene35020 | MS.gene83481 | PPI |
| MS.gene35020 | MS.gene84998 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35020.t1 | MTR_4g094222 | 98.261 | 345 | 5 | 1 | 1 | 344 | 1 | 345 | 0.0 | 707 |
| MS.gene35020.t1 | MTR_5g070680 | 66.570 | 344 | 94 | 3 | 1 | 344 | 1 | 323 | 1.33e-167 | 470 |
| MS.gene35020.t1 | MTR_5g070870 | 71.069 | 318 | 75 | 4 | 29 | 344 | 22 | 324 | 7.09e-160 | 450 |
| MS.gene35020.t1 | MTR_5g026620 | 68.459 | 279 | 86 | 1 | 68 | 344 | 52 | 330 | 9.37e-150 | 425 |
| MS.gene35020.t1 | MTR_5g026620 | 76.991 | 226 | 52 | 0 | 119 | 344 | 37 | 262 | 2.26e-135 | 385 |
| MS.gene35020.t1 | MTR_2g025140 | 26.667 | 180 | 111 | 3 | 158 | 317 | 109 | 287 | 4.79e-14 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35020.t1 | AT5G64210 | 77.500 | 280 | 59 | 2 | 67 | 344 | 76 | 353 | 9.19e-165 | 464 |
| MS.gene35020.t1 | AT3G22370 | 62.349 | 332 | 111 | 4 | 26 | 344 | 24 | 354 | 9.60e-155 | 438 |
| MS.gene35020.t1 | AT3G22360 | 67.616 | 281 | 91 | 0 | 64 | 344 | 45 | 325 | 6.64e-149 | 422 |
| MS.gene35020.t1 | AT3G27620 | 70.342 | 263 | 78 | 0 | 82 | 344 | 67 | 329 | 2.38e-146 | 416 |
| MS.gene35020.t1 | AT1G32350 | 68.045 | 266 | 85 | 0 | 79 | 344 | 53 | 318 | 3.43e-144 | 410 |
| MS.gene35020.t1 | AT1G32350 | 68.016 | 247 | 79 | 0 | 79 | 325 | 53 | 299 | 1.73e-132 | 380 |
| MS.gene35020.t1 | AT4G22260 | 30.000 | 180 | 105 | 3 | 158 | 317 | 121 | 299 | 8.11e-16 | 77.8 |
Find 103 sgRNAs with CRISPR-Local
Find 270 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTATGACATCTTTCAATT+TGG | 0.225219 | 4.4:+20822242 | None:intergenic |
| ACGTGGTTGTGTCGAGTTAT+TGG | 0.242663 | 4.4:-20823444 | MS.gene35020:CDS |
| TTCTGGATAAAGTCGCTTAC+AGG | 0.257073 | 4.4:-20823181 | MS.gene35020:CDS |
| AGCTCCTCTTGGTTATCATT+AGG | 0.258014 | 4.4:-20820557 | None:intergenic |
| ATCTTTCAATTTGGCATCCT+TGG | 0.265673 | 4.4:+20822251 | None:intergenic |
| CGTGGTTGTGTCGAGTTATT+GGG | 0.294734 | 4.4:-20823443 | MS.gene35020:CDS |
| AACTTTGACGGTTAGAATTC+CGG | 0.295362 | 4.4:+20823665 | None:intergenic |
| GCTCCTCTTGGTTATCATTA+GGG | 0.299342 | 4.4:-20820556 | None:intergenic |
| GAAATGGTATGAGAGATTTC+TGG | 0.357389 | 4.4:-20822469 | MS.gene35020:CDS |
| AGAAATCTCTCATACCATTT+CGG | 0.371303 | 4.4:+20822471 | None:intergenic |
| CAAGTTCTACCATAGTCATT+AGG | 0.381374 | 4.4:+20822499 | None:intergenic |
| GAGAAGAAGGAGGAGAGTTC+CGG | 0.382377 | 4.4:-20823478 | MS.gene35020:CDS |
| CAACTCTATGAGCCACCTTT+GGG | 0.383053 | 4.4:+20822391 | None:intergenic |
| ACAACTCTATGAGCCACCTT+TGG | 0.383957 | 4.4:+20822390 | None:intergenic |
| AGCATCCCTCCAACCATTCC+AGG | 0.387944 | 4.4:+20822609 | None:intergenic |
| CTGCCATTGCAATAGACTAT+TGG | 0.388788 | 4.4:-20822281 | MS.gene35020:CDS |
| AGGATGGAACAGAGTGGCCT+TGG | 0.398321 | 4.4:-20823393 | MS.gene35020:CDS |
| CTCTACGCCGATGGCGGAAA+CGG | 0.400110 | 4.4:-20823595 | MS.gene35020:CDS |
| AATGTATAGCTTCTTCTTCA+AGG | 0.401405 | 4.4:+20822361 | None:intergenic |
| ATTTCTGGTTCTTGCTGTAC+AGG | 0.408480 | 4.4:-20822454 | MS.gene35020:CDS |
| TCACAACCGCCGAACTTTGA+CGG | 0.414769 | 4.4:+20823653 | None:intergenic |
| GGCGGTTGTGAGGTCATTCT+CGG | 0.415951 | 4.4:-20823641 | MS.gene35020:CDS |
| TCATTCTCGGCGGCGGAAAT+CGG | 0.425850 | 4.4:-20823628 | MS.gene35020:CDS |
| TTCCTACACTGAGTATTTGA+AGG | 0.429843 | 4.4:-20822340 | MS.gene35020:CDS |
| GAAACAGTTGCAGCTGTCCC+TGG | 0.444443 | 4.4:-20822627 | MS.gene35020:CDS |
| AACCACGTTATTCTTTGTTC+CGG | 0.444667 | 4.4:+20823459 | None:intergenic |
| TCTTTCAATTTGGCATCCTT+GGG | 0.445531 | 4.4:+20822252 | None:intergenic |
| CGCTGATGAGGCTCACCATC+GGG | 0.449836 | 4.4:-20822211 | MS.gene35020:CDS |
| CCATCCTCTCTCATAATCTT+CGG | 0.453617 | 4.4:+20823409 | None:intergenic |
| AAGGTGGCTCATAGAGTTGT+TGG | 0.454036 | 4.4:-20822387 | MS.gene35020:CDS |
| ATGATGCTTATTCAGATCAA+TGG | 0.454262 | 4.4:+20823217 | None:intergenic |
| GCCTCCGTCTCTTCCGCTGA+TGG | 0.455263 | 4.4:+20823520 | None:intergenic |
| GTGGCCTTGGAACTGCTTCA+TGG | 0.462385 | 4.4:-20823380 | MS.gene35020:intron |
| GAGGATGCACCTAATGACTA+TGG | 0.466754 | 4.4:-20822508 | MS.gene35020:CDS |
| GAACCCTCCGTTTCCGCCAT+CGG | 0.466915 | 4.4:+20823588 | None:intergenic |
| GAATTCTAACCGTCAAAGTT+CGG | 0.475373 | 4.4:-20823662 | MS.gene35020:CDS |
| ATTTCAGCATAGTGGTGGTT+GGG | 0.477652 | 4.4:-20822562 | MS.gene35020:CDS |
| GCAGTTCTTTACCGTGGAAG+TGG | 0.484800 | 4.4:+20820590 | None:intergenic |
| TAGAACTTGTGAAGCCGAAA+TGG | 0.485630 | 4.4:-20822485 | MS.gene35020:CDS |
| GCATTCAGCATTGTGTTACA+TGG | 0.485894 | 4.4:-20823710 | MS.gene35020:CDS |
| CCATTGCAATAGACTATTGG+AGG | 0.495126 | 4.4:-20822278 | MS.gene35020:CDS |
| ACTTTATCCAGAAAATTCTT+CGG | 0.501906 | 4.4:+20823191 | None:intergenic |
| AATTTCAGCATAGTGGTGGT+TGG | 0.511730 | 4.4:-20822563 | MS.gene35020:CDS |
| GCTGTCCCTGGAATGGTTGG+AGG | 0.515427 | 4.4:-20822615 | MS.gene35020:CDS |
| TCTATTGCAATGGCAGGAGC+AGG | 0.517572 | 4.4:+20822288 | None:intergenic |
| CGGGACGCACCAGCTCCTCT+TGG | 0.517676 | 4.4:-20820568 | MS.gene35020:CDS |
| CAATAGTCTATTGCAATGGC+AGG | 0.522929 | 4.4:+20822282 | None:intergenic |
| GCCGAGCTTTGATCGGTGGC+CGG | 0.523135 | 4.4:-20823684 | MS.gene35020:CDS |
| TTTCTGGTTCTTGCTGTACA+GGG | 0.524259 | 4.4:-20822453 | MS.gene35020:CDS |
| GACGGAGAAGAACGAGAAGA+AGG | 0.529053 | 4.4:-20823491 | MS.gene35020:CDS |
| TGTGAGGTCATTCTCGGCGG+CGG | 0.532367 | 4.4:-20823635 | MS.gene35020:CDS |
| TAATGATAACCAAGAGGAGC+TGG | 0.534076 | 4.4:+20820559 | None:intergenic |
| CCGAAGATTATGAGAGAGGA+TGG | 0.536815 | 4.4:-20823409 | MS.gene35020:CDS |
| AGGTGGCTCATAGAGTTGTT+GGG | 0.545630 | 4.4:-20822386 | MS.gene35020:CDS |
| TCCGGCCACCGATCAAAGCT+CGG | 0.550176 | 4.4:+20823683 | None:intergenic |
| ACGCCGATGGCGGAAACGGA+GGG | 0.558279 | 4.4:-20823591 | MS.gene35020:CDS |
| ATGGCTCGCCGAGCTTTGAT+CGG | 0.558446 | 4.4:-20823691 | MS.gene35020:CDS |
| AACAGTCATTCGCGCTGATG+AGG | 0.563488 | 4.4:-20822223 | MS.gene35020:CDS |
| TACGCCGATGGCGGAAACGG+AGG | 0.565606 | 4.4:-20823592 | MS.gene35020:CDS |
| CTTCCACGGTAAAGAACTGC+GGG | 0.566723 | 4.4:-20820587 | MS.gene35020:CDS |
| GTGGTTGTGTCGAGTTATTG+GGG | 0.571030 | 4.4:-20823442 | MS.gene35020:CDS |
| GCAGCTGTCCCTGGAATGGT+TGG | 0.572427 | 4.4:-20822618 | MS.gene35020:CDS |
| CAGAGGCATCTCTACGCCGA+TGG | 0.573886 | 4.4:-20823604 | MS.gene35020:CDS |
| TTGAAGAAGCAGAGAATGAG+AGG | 0.575067 | 4.4:-20822527 | MS.gene35020:CDS |
| TCTTCCGCTGATGGCTTCGA+CGG | 0.586594 | 4.4:+20823529 | None:intergenic |
| GCCATCAGCGGAAGAGACGG+AGG | 0.587726 | 4.4:-20823521 | MS.gene35020:CDS |
| TATTTGAAGGACATTGATAG+TGG | 0.587742 | 4.4:-20822327 | MS.gene35020:CDS |
| TTTGATTGATAAGTCTCCCA+TGG | 0.588432 | 4.4:+20823245 | None:intergenic |
| GAAGCCATCAGCGGAAGAGA+CGG | 0.589166 | 4.4:-20823524 | MS.gene35020:CDS |
| GCGCTGATGAGGCTCACCAT+CGG | 0.590135 | 4.4:-20822212 | MS.gene35020:CDS |
| CGAAAATTTCAGCATAGTGG+TGG | 0.590958 | 4.4:-20822567 | MS.gene35020:CDS |
| CTGTTTCGAGCATCATTGCA+CGG | 0.591594 | 4.4:+20822643 | None:intergenic |
| GTCAAAGTTCGGCGGTTGTG+AGG | 0.592006 | 4.4:-20823651 | MS.gene35020:CDS |
| TTACATACTTTCCCCAAAGG+TGG | 0.592089 | 4.4:-20822403 | MS.gene35020:CDS |
| CTGTCCCTGGAATGGTTGGA+GGG | 0.593126 | 4.4:-20822614 | MS.gene35020:CDS |
| CTCCGAAAATTTCAGCATAG+TGG | 0.594257 | 4.4:-20822570 | MS.gene35020:CDS |
| AACTCTATGAGCCACCTTTG+GGG | 0.595501 | 4.4:+20822392 | None:intergenic |
| ACTTTACATACTTTCCCCAA+AGG | 0.601341 | 4.4:-20822406 | MS.gene35020:CDS |
| CATCGGCGTAGAGATGCCTC+TGG | 0.604415 | 4.4:+20823605 | None:intergenic |
| ATGCAGGATATCCACTTCCA+CGG | 0.604624 | 4.4:-20820601 | MS.gene35020:intron |
| AGGCATCTCTACGCCGATGG+CGG | 0.606114 | 4.4:-20823601 | MS.gene35020:CDS |
| AGAGACGGAGGCGAAATCGA+CGG | 0.606512 | 4.4:-20823509 | MS.gene35020:CDS |
| CGGCGGCGGAAATCGGCCAG+AGG | 0.611702 | 4.4:-20823621 | MS.gene35020:CDS |
| GAGGCCGAAGATTATGAGAG+AGG | 0.612635 | 4.4:-20823413 | MS.gene35020:CDS |
| TGCGCCGTCGAAGCCATCAG+CGG | 0.613702 | 4.4:-20823533 | MS.gene35020:CDS |
| GGAGAAGAACGAGAAGAAGG+AGG | 0.617945 | 4.4:-20823488 | MS.gene35020:CDS |
| GTTACCATGAAGCAGTTCCA+AGG | 0.624690 | 4.4:+20823376 | None:intergenic |
| GCTCGCCGAGCTTTGATCGG+TGG | 0.625356 | 4.4:-20823688 | MS.gene35020:CDS |
| AGTTGCAGCTGTCCCTGGAA+TGG | 0.626357 | 4.4:-20822622 | MS.gene35020:CDS |
| AGACTATTGGAGGCTTCCCA+AGG | 0.628882 | 4.4:-20822268 | MS.gene35020:CDS |
| GCATCCCTCCAACCATTCCA+GGG | 0.636143 | 4.4:+20822610 | None:intergenic |
| GAACACTCACTGAAGCGAAG+TGG | 0.637444 | 4.4:+20822181 | None:intergenic |
| GTCCTTCAAATACTCAGTGT+AGG | 0.637701 | 4.4:+20822338 | None:intergenic |
| CGTCCCGCAGTTCTTTACCG+TGG | 0.639191 | 4.4:+20820584 | None:intergenic |
| CCTCCAATAGTCTATTGCAA+TGG | 0.640373 | 4.4:+20822278 | None:intergenic |
| TTCTAACCGTCAAAGTTCGG+CGG | 0.651224 | 4.4:-20823659 | MS.gene35020:CDS |
| TGGTTGTGTCGAGTTATTGG+GGG | 0.651952 | 4.4:-20823441 | MS.gene35020:CDS |
| CGAAGTGGTTCACATCCCGA+TGG | 0.653019 | 4.4:+20822196 | None:intergenic |
| AAGCCCTAATGATAACCAAG+AGG | 0.665170 | 4.4:+20820553 | None:intergenic |
| ACTTCCACGGTAAAGAACTG+CGG | 0.706975 | 4.4:-20820588 | MS.gene35020:CDS |
| GGTTGTGAGGTCATTCTCGG+CGG | 0.726894 | 4.4:-20823638 | MS.gene35020:CDS |
| TGAGAGAGGATGGAACAGAG+TGG | 0.750950 | 4.4:-20823399 | MS.gene35020:CDS |
| TTCCGGAACAAAGAATAACG+TGG | 0.755903 | 4.4:-20823461 | MS.gene35020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATCATCTTACATTTATTTA+CGG | - | chr4.4:20822173-20822192 | MS.gene35020:intron | 15.0% |
| !!! | TAATTTAGTCAATTTTTTAC+CGG | - | chr4.4:20821282-20821301 | MS.gene35020:intron | 15.0% |
| !!! | TCATAGTTGTTTTAAAATAA+AGG | - | chr4.4:20823523-20823542 | MS.gene35020:CDS | 15.0% |
| !! | ACATGAAAACTGTAAAATTT+AGG | - | chr4.4:20822282-20822301 | MS.gene35020:CDS | 20.0% |
| !! | ATAAATGTAAGATGATATGT+TGG | + | chr4.4:20822170-20822189 | None:intergenic | 20.0% |
| !! | GGAATTGTATTATAAAAACA+GGG | + | chr4.4:20823644-20823663 | None:intergenic | 20.0% |
| !! | TTTGCTACATATGAATAAAA+GGG | - | chr4.4:20821370-20821389 | MS.gene35020:intron | 20.0% |
| !!! | AAAACTGTAAAATTTAGGAT+TGG | - | chr4.4:20822287-20822306 | MS.gene35020:CDS | 20.0% |
| !!! | AATTATTTTAAGTGATGTTG+TGG | - | chr4.4:20823053-20823072 | MS.gene35020:intron | 20.0% |
| !!! | GTCATATTTTATTTTATTCC+TGG | - | chr4.4:20821433-20821452 | MS.gene35020:intron | 20.0% |
| !!! | TAAAATTTAGGATTGGTATA+AGG | - | chr4.4:20822294-20822313 | MS.gene35020:CDS | 20.0% |
| !!! | TTACTTATATCCAACTTTTA+GGG | - | chr4.4:20822546-20822565 | MS.gene35020:CDS | 20.0% |
| !!! | TTTACTTATATCCAACTTTT+AGG | - | chr4.4:20822545-20822564 | MS.gene35020:CDS | 20.0% |
| !!! | TTTCTTTTTCATAATAATGC+TGG | - | chr4.4:20823596-20823615 | MS.gene35020:CDS | 20.0% |
| ! | AAAAAAAATTGTAACCACCT+TGG | - | chr4.4:20823386-20823405 | MS.gene35020:CDS | 25.0% |
| ! | AACTTGTGATCTCAATATAT+TGG | - | chr4.4:20822235-20822254 | MS.gene35020:CDS | 25.0% |
| ! | AATTCATGGTTTCTATAAAG+TGG | - | chr4.4:20820966-20820985 | MS.gene35020:intron | 25.0% |
| ! | ACTTTATCCAGAAAATTCTT+CGG | + | chr4.4:20821085-20821104 | None:intergenic | 25.0% |
| ! | AGAACTTAGTTTAAAGAAAG+TGG | - | chr4.4:20823496-20823515 | MS.gene35020:CDS | 25.0% |
| ! | ATAAGTAAAGCTTCATTGTT+TGG | + | chr4.4:20822534-20822553 | None:intergenic | 25.0% |
| ! | ATTGACTAAATTACATGTGT+CGG | + | chr4.4:20821275-20821294 | None:intergenic | 25.0% |
| ! | ATTGTATGATCCATAACAAA+AGG | - | chr4.4:20821196-20821215 | MS.gene35020:intron | 25.0% |
| ! | CGGAATTGTATTATAAAAAC+AGG | + | chr4.4:20823645-20823664 | None:intergenic | 25.0% |
| ! | GTATAAGGTAATATACACAA+AGG | - | chr4.4:20822309-20822328 | MS.gene35020:CDS | 25.0% |
| ! | TAAGTAAAGCTTCATTGTTT+GGG | + | chr4.4:20822533-20822552 | None:intergenic | 25.0% |
| ! | TGTTATGACATCTTTCAATT+TGG | + | chr4.4:20822034-20822053 | None:intergenic | 25.0% |
| ! | TTTATGTGCTCTTAAGAAAT+TGG | - | chr4.4:20821400-20821419 | MS.gene35020:intron | 25.0% |
| !! | GTTTGCTACATATGAATAAA+AGG | - | chr4.4:20821369-20821388 | MS.gene35020:intron | 25.0% |
| !! | TGTAGTGCTTAATTATGTTT+TGG | - | chr4.4:20823137-20823156 | MS.gene35020:intron | 25.0% |
| !! | TTTGTTATGGATCATACAAT+AGG | + | chr4.4:20821196-20821215 | None:intergenic | 25.0% |
| !!! | ATTGTTTTTTCGCTAATTCA+TGG | - | chr4.4:20820952-20820971 | MS.gene35020:intron | 25.0% |
| !!! | GTAAGAAATTTTAACCACTT+GGG | - | chr4.4:20822571-20822590 | MS.gene35020:CDS | 25.0% |
| !!! | TTTTGTTATGATGTTCTTCA+TGG | - | chr4.4:20821548-20821567 | MS.gene35020:intron | 25.0% |
| AATGTATAGCTTCTTCTTCA+AGG | + | chr4.4:20821915-20821934 | None:intergenic | 30.0% | |
| ACCTTAAAAAACACATCAGT+GGG | + | chr4.4:20821139-20821158 | None:intergenic | 30.0% | |
| AGAAATCTCTCATACCATTT+CGG | + | chr4.4:20821805-20821824 | None:intergenic | 30.0% | |
| AGCATAAAACATAGCAATAC+TGG | + | chr4.4:20823475-20823494 | None:intergenic | 30.0% | |
| ATAACATCACAATTCGATCT+TGG | + | chr4.4:20823230-20823249 | None:intergenic | 30.0% | |
| ATGATGCTTATTCAGATCAA+TGG | + | chr4.4:20821059-20821078 | None:intergenic | 30.0% | |
| ATTCCAAATAACAGTGCAAA+AGG | - | chr4.4:20822727-20822746 | MS.gene35020:intron | 30.0% | |
| CATACTATACTAGATATTGC+AGG | - | chr4.4:20821513-20821532 | MS.gene35020:intron | 30.0% | |
| TAAGTATATGTCATCTTCCA+AGG | + | chr4.4:20823406-20823425 | None:intergenic | 30.0% | |
| TATCAATGATGCATATTTCC+AGG | + | chr4.4:20821454-20821473 | None:intergenic | 30.0% | |
| TATTTGAAGGACATTGATAG+TGG | - | chr4.4:20821946-20821965 | MS.gene35020:intron | 30.0% | |
| TGTAATCTTAGGAAAGTAGT+GGG | - | chr4.4:20822955-20822974 | MS.gene35020:intron | 30.0% | |
| TTGTAATCTTAGGAAAGTAG+TGG | - | chr4.4:20822954-20822973 | MS.gene35020:intron | 30.0% | |
| ! | AACGTCTCTGAAAATATTCA+TGG | + | chr4.4:20821603-20821622 | None:intergenic | 30.0% |
| ! | AGAAGTATATGACTACCATA+AGG | - | chr4.4:20822445-20822464 | MS.gene35020:CDS | 30.0% |
| ! | GAATATTTTCAGAGACGTTA+TGG | - | chr4.4:20821604-20821623 | MS.gene35020:intron | 30.0% |
| ! | TCTTCTCTTTCCTTTTGTTA+TGG | + | chr4.4:20821209-20821228 | None:intergenic | 30.0% |
| !! | ATTTTAAGTGATGTTGTGGA+TGG | - | chr4.4:20823057-20823076 | MS.gene35020:intron | 30.0% |
| !! | GAGTAGTTTCTGTTTTATTG+TGG | - | chr4.4:20823197-20823216 | MS.gene35020:CDS | 30.0% |
| !! | GGTAAGAAATTTTAACCACT+TGG | - | chr4.4:20822570-20822589 | MS.gene35020:CDS | 30.0% |
| !! | TATTGCAGTACACCTTATTT+TGG | - | chr4.4:20823271-20823290 | MS.gene35020:intron | 30.0% |
| !! | TTTTAAGTGATGTTGTGGAT+GGG | - | chr4.4:20823058-20823077 | MS.gene35020:intron | 30.0% |
| !!! | ATTGCAGTACACCTTATTTT+GGG | - | chr4.4:20823272-20823291 | MS.gene35020:intron | 30.0% |
| !!! | GTGCTTTGATTTGTTCTTTT+AGG | - | chr4.4:20823443-20823462 | MS.gene35020:CDS | 30.0% |
| !!! | GTTATGCATTAGTTTTCGTT+TGG | - | chr4.4:20823245-20823264 | MS.gene35020:CDS | 30.0% |
| AACCACGTTATTCTTTGTTC+CGG | + | chr4.4:20820817-20820836 | None:intergenic | 35.0% | |
| AACTTTGACGGTTAGAATTC+CGG | + | chr4.4:20820611-20820630 | None:intergenic | 35.0% | |
| AAGTGCATGAGAGGAAATTA+GGG | + | chr4.4:20822368-20822387 | None:intergenic | 35.0% | |
| ACACTACACTTTCGAATTCT+AGG | + | chr4.4:20822391-20822410 | None:intergenic | 35.0% | |
| ACTGCATCTTCTTAGAATCT+GGG | + | chr4.4:20823024-20823043 | None:intergenic | 35.0% | |
| ACTTTACATACTTTCCCCAA+AGG | - | chr4.4:20821867-20821886 | MS.gene35020:intron | 35.0% | |
| ATCTTCTTAGAATCTGGGTT+TGG | + | chr4.4:20823019-20823038 | None:intergenic | 35.0% | |
| ATCTTTCAATTTGGCATCCT+TGG | + | chr4.4:20822025-20822044 | None:intergenic | 35.0% | |
| ATTGAGATCACAAGTTCAAC+AGG | + | chr4.4:20822231-20822250 | None:intergenic | 35.0% | |
| CAAGTTCTACCATAGTCATT+AGG | + | chr4.4:20821777-20821796 | None:intergenic | 35.0% | |
| CACCTTAAAAAACACATCAG+TGG | + | chr4.4:20821140-20821159 | None:intergenic | 35.0% | |
| GAAATGGTATGAGAGATTTC+TGG | - | chr4.4:20821804-20821823 | MS.gene35020:intron | 35.0% | |
| GAATTCTAACCGTCAAAGTT+CGG | - | chr4.4:20820611-20820630 | MS.gene35020:intron | 35.0% | |
| GACAACAAATGTATGCCTTA+TGG | + | chr4.4:20822463-20822482 | None:intergenic | 35.0% | |
| GAGATCCAATACCCAAAATA+AGG | + | chr4.4:20823286-20823305 | None:intergenic | 35.0% | |
| GATAACGAACATGCTAAATC+AGG | + | chr4.4:20823552-20823571 | None:intergenic | 35.0% | |
| GTAATCTTAGGAAAGTAGTG+GGG | - | chr4.4:20822956-20822975 | MS.gene35020:intron | 35.0% | |
| GTTGCTATACACTCTACAAA+GGG | - | chr4.4:20822418-20822437 | MS.gene35020:CDS | 35.0% | |
| TAATCTTAGGAAAGTAGTGG+GGG | - | chr4.4:20822957-20822976 | MS.gene35020:intron | 35.0% | |
| TCTTTCAATTTGGCATCCTT+GGG | + | chr4.4:20822024-20822043 | None:intergenic | 35.0% | |
| TGTTGCTATACACTCTACAA+AGG | - | chr4.4:20822417-20822436 | MS.gene35020:CDS | 35.0% | |
| TTCATAATAATGCTGGCCAT+TGG | - | chr4.4:20823603-20823622 | MS.gene35020:CDS | 35.0% | |
| TTCCTACACTGAGTATTTGA+AGG | - | chr4.4:20821933-20821952 | MS.gene35020:intron | 35.0% | |
| TTTAAGTGATGTTGTGGATG+GGG | - | chr4.4:20823059-20823078 | MS.gene35020:intron | 35.0% | |
| TTTCTTACCTCCCTAAAAGT+TGG | + | chr4.4:20822559-20822578 | None:intergenic | 35.0% | |
| TTTGATTGATAAGTCTCCCA+TGG | + | chr4.4:20821031-20821050 | None:intergenic | 35.0% | |
| ! | AAATTACATGTGTCGGTGTA+AGG | + | chr4.4:20821268-20821287 | None:intergenic | 35.0% |
| ! | AGAATTCGAAAGTGTAGTGT+AGG | - | chr4.4:20822391-20822410 | MS.gene35020:CDS | 35.0% |
| ! | CACCACTATGCTGAAATTTT+CGG | + | chr4.4:20821708-20821727 | None:intergenic | 35.0% |
| ! | CATCCTTTTGCACTGTTATT+TGG | + | chr4.4:20822733-20822752 | None:intergenic | 35.0% |
| !! | AAGGGAGTGATATGTCATTT+TGG | - | chr4.4:20822651-20822670 | MS.gene35020:CDS | 35.0% |
| !! | AGAAATTTTAACCACTTGGG+AGG | - | chr4.4:20822574-20822593 | MS.gene35020:CDS | 35.0% |
| !! | CTTATATCCAACTTTTAGGG+AGG | - | chr4.4:20822549-20822568 | MS.gene35020:CDS | 35.0% |
| !!! | GATGTGTTTTTTAAGGTGTG+TGG | - | chr4.4:20821142-20821161 | MS.gene35020:intron | 35.0% |
| !!! | GTACACCTTATTTTGGGTAT+TGG | - | chr4.4:20823278-20823297 | MS.gene35020:intron | 35.0% |
| !!! | TCCCACTGATGTGTTTTTTA+AGG | - | chr4.4:20821135-20821154 | MS.gene35020:intron | 35.0% |
| AACGAACATGCTAAATCAGG+TGG | + | chr4.4:20823549-20823568 | None:intergenic | 40.0% | |
| AATTAGGGGAAAATGCGATC+TGG | + | chr4.4:20822353-20822372 | None:intergenic | 40.0% | |
| AATTTCAGCATAGTGGTGGT+TGG | - | chr4.4:20821710-20821729 | MS.gene35020:intron | 40.0% | |
| ACAATTCCGTTCTTTCATGC+AGG | - | chr4.4:20823656-20823675 | MS.gene35020:CDS | 40.0% | |
| AGTGCAAAAGGATGCTAATG+TGG | - | chr4.4:20822739-20822758 | MS.gene35020:intron | 40.0% | |
| AGTGCATGAGAGGAAATTAG+GGG | + | chr4.4:20822367-20822386 | None:intergenic | 40.0% | |
| ATCAACCGTGAGAACTTCAA+GGG | - | chr4.4:20822864-20822883 | MS.gene35020:intron | 40.0% | |
| ATTTCAGCATAGTGGTGGTT+GGG | - | chr4.4:20821711-20821730 | MS.gene35020:intron | 40.0% | |
| CAAGAAACAGCCACTGATAT+CGG | + | chr4.4:20823174-20823193 | None:intergenic | 40.0% | |
| CAATAGTCTATTGCAATGGC+AGG | + | chr4.4:20821994-20822013 | None:intergenic | 40.0% | |
| CAATTTGATGCCGATATCAG+TGG | - | chr4.4:20823161-20823180 | MS.gene35020:CDS | 40.0% | |
| CACTGATATCGGCATCAAAT+TGG | + | chr4.4:20823163-20823182 | None:intergenic | 40.0% | |
| CACTGCATCTTCTTAGAATC+TGG | + | chr4.4:20823025-20823044 | None:intergenic | 40.0% | |
| CCATCCTCTCTCATAATCTT+CGG | + | chr4.4:20820867-20820886 | None:intergenic | 40.0% | |
| CCATTGCAATAGACTATTGG+AGG | - | chr4.4:20821995-20822014 | MS.gene35020:intron | 40.0% | |
| CCTCCAATAGTCTATTGCAA+TGG | + | chr4.4:20821998-20822017 | None:intergenic | 40.0% | |
| CGAAAATTTCAGCATAGTGG+TGG | - | chr4.4:20821706-20821725 | MS.gene35020:intron | 40.0% | |
| CTCCGAAAATTTCAGCATAG+TGG | - | chr4.4:20821703-20821722 | MS.gene35020:intron | 40.0% | |
| CTGCCATTGCAATAGACTAT+TGG | - | chr4.4:20821992-20822011 | MS.gene35020:intron | 40.0% | |
| GAAGTGCATGAGAGGAAATT+AGG | + | chr4.4:20822369-20822388 | None:intergenic | 40.0% | |
| GAGAAAGTCAATCCATTGGA+AGG | - | chr4.4:20822814-20822833 | MS.gene35020:intron | 40.0% | |
| GCATTCAGCATTGTGTTACA+TGG | - | chr4.4:20820563-20820582 | MS.gene35020:CDS | 40.0% | |
| GGATATCCTGCATGAAAGAA+CGG | + | chr4.4:20823665-20823684 | None:intergenic | 40.0% | |
| GTATATGTCATCTTCCAAGG+TGG | + | chr4.4:20823403-20823422 | None:intergenic | 40.0% | |
| GTCCTTCAAATACTCAGTGT+AGG | + | chr4.4:20821938-20821957 | None:intergenic | 40.0% | |
| GTGCAAAAGGATGCTAATGT+GGG | - | chr4.4:20822740-20822759 | MS.gene35020:intron | 40.0% | |
| TAATGATAACCAAGAGGAGC+TGG | + | chr4.4:20823717-20823736 | None:intergenic | 40.0% | |
| TAGAACTTGTGAAGCCGAAA+TGG | - | chr4.4:20821788-20821807 | MS.gene35020:intron | 40.0% | |
| TAGAATCTGGGTTTGGACTT+TGG | + | chr4.4:20823012-20823031 | None:intergenic | 40.0% | |
| TGCAAAAGGATGCTAATGTG+GGG | - | chr4.4:20822741-20822760 | MS.gene35020:intron | 40.0% | |
| TTACATACTTTCCCCAAAGG+TGG | - | chr4.4:20821870-20821889 | MS.gene35020:intron | 40.0% | |
| TTCCGGAACAAAGAATAACG+TGG | - | chr4.4:20820812-20820831 | MS.gene35020:intron | 40.0% | |
| TTCTGGATAAAGTCGCTTAC+AGG | - | chr4.4:20821092-20821111 | MS.gene35020:intron | 40.0% | |
| TTGAAGAAGCAGAGAATGAG+AGG | - | chr4.4:20821746-20821765 | MS.gene35020:intron | 40.0% | |
| ! | AATTCTAGGAAGTGCATGAG+AGG | + | chr4.4:20822377-20822396 | None:intergenic | 40.0% |
| ! | GAAATTTTCGGAGTGACTTG+AGG | + | chr4.4:20821696-20821715 | None:intergenic | 40.0% |
| ! | GCTGGTTCTCTTGTAATCTT+AGG | - | chr4.4:20822944-20822963 | MS.gene35020:intron | 40.0% |
| ! | TCATGTGCCGAAGAATTTTC+TGG | - | chr4.4:20821075-20821094 | MS.gene35020:intron | 40.0% |
| ! | TCGTTGTTGATTGTAGCCAT+GGG | - | chr4.4:20821012-20821031 | MS.gene35020:intron | 40.0% |
| ! | TTCGTTGTTGATTGTAGCCA+TGG | - | chr4.4:20821011-20821030 | MS.gene35020:intron | 40.0% |
| !! | ATATCAGTGGCTGTTTCTTG+AGG | - | chr4.4:20823174-20823193 | MS.gene35020:CDS | 40.0% |
| !! | ATTTCTGGTTCTTGCTGTAC+AGG | - | chr4.4:20821819-20821838 | MS.gene35020:intron | 40.0% |
| !! | ATTTTAACCACTTGGGAGGA+CGG | - | chr4.4:20822578-20822597 | MS.gene35020:CDS | 40.0% |
| !! | TATCAGTGGCTGTTTCTTGA+GGG | - | chr4.4:20823175-20823194 | MS.gene35020:CDS | 40.0% |
| !! | TTTCTGGTTCTTGCTGTACA+GGG | - | chr4.4:20821820-20821839 | MS.gene35020:intron | 40.0% |
| !! | TTTGGGTATTGGATCTCGTT+TGG | - | chr4.4:20823289-20823308 | MS.gene35020:intron | 40.0% |
| !!! | GTCATTTTGGAGTGATCCAT+GGG | - | chr4.4:20822664-20822683 | MS.gene35020:intron | 40.0% |
| !!! | TGTCATTTTGGAGTGATCCA+TGG | - | chr4.4:20822663-20822682 | MS.gene35020:intron | 40.0% |
| AACTCTATGAGCCACCTTTG+GGG | + | chr4.4:20821884-20821903 | None:intergenic | 45.0% | |
| AAGGTGGCTCATAGAGTTGT+TGG | - | chr4.4:20821886-20821905 | MS.gene35020:intron | 45.0% | |
| ACAACTCTATGAGCCACCTT+TGG | + | chr4.4:20821886-20821905 | None:intergenic | 45.0% | |
| ACGTGGTTGTGTCGAGTTAT+TGG | - | chr4.4:20820829-20820848 | MS.gene35020:intron | 45.0% | |
| ACTCAACAGACAAATGGGTC+TGG | - | chr4.4:20822889-20822908 | MS.gene35020:intron | 45.0% | |
| ACTTCCACGGTAAAGAACTG+CGG | - | chr4.4:20823685-20823704 | MS.gene35020:CDS | 45.0% | |
| AGGAGGAGAATTAAACGAGC+TGG | - | chr4.4:20822926-20822945 | MS.gene35020:intron | 45.0% | |
| AGGTGGCTCATAGAGTTGTT+GGG | - | chr4.4:20821887-20821906 | MS.gene35020:intron | 45.0% | |
| AGTAAGTGCAGGGAAGTAGT+GGG | - | chr4.4:20822626-20822645 | MS.gene35020:CDS | 45.0% | |
| AGTACCCCTTGAAGTTCTCA+CGG | + | chr4.4:20822872-20822891 | None:intergenic | 45.0% | |
| ATGCAGGATATCCACTTCCA+CGG | - | chr4.4:20823672-20823691 | MS.gene35020:CDS | 45.0% | |
| CAACTCTATGAGCCACCTTT+GGG | + | chr4.4:20821885-20821904 | None:intergenic | 45.0% | |
| CATTCACCAACTCCTTCCAA+TGG | + | chr4.4:20822829-20822848 | None:intergenic | 45.0% | |
| CCGAAGATTATGAGAGAGGA+TGG | - | chr4.4:20820864-20820883 | MS.gene35020:intron | 45.0% | |
| CGTGGTTGTGTCGAGTTATT+GGG | - | chr4.4:20820830-20820849 | MS.gene35020:intron | 45.0% | |
| CTGTTTCGAGCATCATTGCA+CGG | + | chr4.4:20821633-20821652 | None:intergenic | 45.0% | |
| GAACACCAGTGTGAAACCAA+TGG | + | chr4.4:20823622-20823641 | None:intergenic | 45.0% | |
| GACACGACACAACATTGACA+CGG | + | chr4.4:20821319-20821338 | None:intergenic | 45.0% | |
| GAGGATGCACCTAATGACTA+TGG | - | chr4.4:20821765-20821784 | MS.gene35020:intron | 45.0% | |
| GATCAACCGTGAGAACTTCA+AGG | - | chr4.4:20822863-20822882 | MS.gene35020:intron | 45.0% | |
| GATGTTGTGGATGGGGTTTA+TGG | - | chr4.4:20823066-20823085 | MS.gene35020:intron | 45.0% | |
| GCAAAAGGATGCTAATGTGG+GGG | - | chr4.4:20822742-20822761 | MS.gene35020:intron | 45.0% | |
| GCTATACACTCTACAAAGGG+CGG | - | chr4.4:20822421-20822440 | MS.gene35020:CDS | 45.0% | |
| GGAGGAGAAAGTCAATCCAT+TGG | - | chr4.4:20822810-20822829 | MS.gene35020:intron | 45.0% | |
| GTCAATCCATTGGAAGGAGT+TGG | - | chr4.4:20822820-20822839 | MS.gene35020:intron | 45.0% | |
| GTCGTGTCTAGTGTTCATGT+TGG | - | chr4.4:20821331-20821350 | MS.gene35020:intron | 45.0% | |
| GTGGTTGTGTCGAGTTATTG+GGG | - | chr4.4:20820831-20820850 | MS.gene35020:intron | 45.0% | |
| GTGTGGAACAAGTTCAGAGA+GGG | - | chr4.4:20822769-20822788 | MS.gene35020:intron | 45.0% | |
| GTTACCATGAAGCAGTTCCA+AGG | + | chr4.4:20820900-20820919 | None:intergenic | 45.0% | |
| TAGTAAGTGCAGGGAAGTAG+TGG | - | chr4.4:20822625-20822644 | MS.gene35020:CDS | 45.0% | |
| TCAACCGTGAGAACTTCAAG+GGG | - | chr4.4:20822865-20822884 | MS.gene35020:intron | 45.0% | |
| TGGTTGTGTCGAGTTATTGG+GGG | - | chr4.4:20820832-20820851 | MS.gene35020:intron | 45.0% | |
| TGTGGAACAAGTTCAGAGAG+GGG | - | chr4.4:20822770-20822789 | MS.gene35020:intron | 45.0% | |
| TGTGGATGGGGTTTATGGAA+TGG | - | chr4.4:20823071-20823090 | MS.gene35020:intron | 45.0% | |
| TTCTAACCGTCAAAGTTCGG+CGG | - | chr4.4:20820614-20820633 | MS.gene35020:intron | 45.0% | |
| ! | AGGGGACTGATACGTTGTTT+TGG | - | chr4.4:20822980-20822999 | MS.gene35020:intron | 45.0% |
| ! | CTGGTTCTCTAGTAAGTGCA+GGG | - | chr4.4:20822616-20822635 | MS.gene35020:CDS | 45.0% |
| ! | GAAACGGAGGGTTCTTTTAC+TGG | - | chr4.4:20820694-20820713 | MS.gene35020:intron | 45.0% |
| ! | GAGGGTTCTTTTACTGGAAG+AGG | - | chr4.4:20820700-20820719 | MS.gene35020:intron | 45.0% |
| ! | GGGGTACTCAACAGACAAAT+GGG | - | chr4.4:20822884-20822903 | MS.gene35020:intron | 45.0% |
| ! | TTTGGAGTGATCCATGGGTA+GGG | - | chr4.4:20822669-20822688 | MS.gene35020:intron | 45.0% |
| ! | TTTTAACCACTTGGGAGGAC+GGG | - | chr4.4:20822579-20822598 | MS.gene35020:CDS | 45.0% |
| !! | AGGGGTACTCAACAGACAAA+TGG | - | chr4.4:20822883-20822902 | MS.gene35020:intron | 45.0% |
| !!! | TTTTGGAGTGATCCATGGGT+AGG | - | chr4.4:20822668-20822687 | MS.gene35020:intron | 45.0% |
| AAAGGATGCTAATGTGGGGG+AGG | - | chr4.4:20822745-20822764 | MS.gene35020:intron | 50.0% | |
| ACAAGTTCAGAGAGGGGTGA+AGG | - | chr4.4:20822776-20822795 | MS.gene35020:intron | 50.0% | |
| AGACTATTGGAGGCTTCCCA+AGG | - | chr4.4:20822005-20822024 | MS.gene35020:intron | 50.0% | |
| AGAGAGGGGTGAAGGAAAGT+GGG | - | chr4.4:20822784-20822803 | MS.gene35020:intron | 50.0% | |
| CGAGTTATTGGGGGATTTCG+AGG | - | chr4.4:20820841-20820860 | MS.gene35020:intron | 50.0% | |
| CGTGTGGAACAAGTTCAGAG+AGG | - | chr4.4:20822768-20822787 | MS.gene35020:intron | 50.0% | |
| CTTCCACGGTAAAGAACTGC+GGG | - | chr4.4:20823686-20823705 | MS.gene35020:CDS | 50.0% | |
| GAACACTCACTGAAGCGAAG+TGG | + | chr4.4:20822095-20822114 | None:intergenic | 50.0% | |
| GACGGAGAAGAACGAGAAGA+AGG | - | chr4.4:20820782-20820801 | MS.gene35020:intron | 50.0% | |
| GAGAAGAAGGAGGAGAGTTC+CGG | - | chr4.4:20820795-20820814 | MS.gene35020:intron | 50.0% | |
| GAGGCCGAAGATTATGAGAG+AGG | - | chr4.4:20820860-20820879 | MS.gene35020:intron | 50.0% | |
| GCAGTTCTTTACCGTGGAAG+TGG | + | chr4.4:20823686-20823705 | None:intergenic | 50.0% | |
| GGAGAAGAACGAGAAGAAGG+AGG | - | chr4.4:20820785-20820804 | MS.gene35020:intron | 50.0% | |
| GTAAGTGCAGGGAAGTAGTG+GGG | - | chr4.4:20822627-20822646 | MS.gene35020:CDS | 50.0% | |
| GTGAAGGAAAGTGGGATTGG+AGG | - | chr4.4:20822792-20822811 | MS.gene35020:intron | 50.0% | |
| TAAGTGCAGGGAAGTAGTGG+GGG | - | chr4.4:20822628-20822647 | MS.gene35020:CDS | 50.0% | |
| TAAGTGTTCTCTGTCGCACG+AGG | - | chr4.4:20821231-20821250 | MS.gene35020:intron | 50.0% | |
| TCACAACCGCCGAACTTTGA+CGG | + | chr4.4:20820623-20820642 | None:intergenic | 50.0% | |
| TCTATTGCAATGGCAGGAGC+AGG | + | chr4.4:20821988-20822007 | None:intergenic | 50.0% | |
| TGAGAGAGGATGGAACAGAG+TGG | - | chr4.4:20820874-20820893 | MS.gene35020:intron | 50.0% | |
| TTTAACCACTTGGGAGGACG+GGG | - | chr4.4:20822580-20822599 | MS.gene35020:CDS | 50.0% | |
| ! | AACAGTCATTCGCGCTGATG+AGG | - | chr4.4:20822050-20822069 | MS.gene35020:intron | 50.0% |
| ! | CTTAGGAAAGTAGTGGGGGA+GGG | - | chr4.4:20822961-20822980 | MS.gene35020:intron | 50.0% |
| ! | GCTGGTTCTCTAGTAAGTGC+AGG | - | chr4.4:20822615-20822634 | MS.gene35020:CDS | 50.0% |
| ! | TCTTAGGAAAGTAGTGGGGG+AGG | - | chr4.4:20822960-20822979 | MS.gene35020:intron | 50.0% |
| ! | TTAGGAAAGTAGTGGGGGAG+GGG | - | chr4.4:20822962-20822981 | MS.gene35020:intron | 50.0% |
| !! | GGGTATTGGATCTCGTTTGG+AGG | - | chr4.4:20823292-20823311 | MS.gene35020:intron | 50.0% |
| ACGGGGAGAGTCAAACAAGC+TGG | - | chr4.4:20822597-20822616 | MS.gene35020:CDS | 55.0% | |
| AGACAAATGGGTCTGGCTGC+GGG | - | chr4.4:20822896-20822915 | MS.gene35020:intron | 55.0% | |
| AGAGACGGAGGCGAAATCGA+CGG | - | chr4.4:20820764-20820783 | MS.gene35020:intron | 55.0% | |
| AGCATCCCTCCAACCATTCC+AGG | + | chr4.4:20821667-20821686 | None:intergenic | 55.0% | |
| AGGATGGAACAGAGTGGCCT+TGG | - | chr4.4:20820880-20820899 | MS.gene35020:intron | 55.0% | |
| AGTGATCCATGGGTAGGGCA+AGG | - | chr4.4:20822674-20822693 | MS.gene35020:intron | 55.0% | |
| AGTTGCAGCTGTCCCTGGAA+TGG | - | chr4.4:20821651-20821670 | MS.gene35020:intron | 55.0% | |
| CAGACAAATGGGTCTGGCTG+CGG | - | chr4.4:20822895-20822914 | MS.gene35020:intron | 55.0% | |
| CAGAGAGGGGTGAAGGAAAG+TGG | - | chr4.4:20822783-20822802 | MS.gene35020:intron | 55.0% | |
| CGAAGTGGTTCACATCCCGA+TGG | + | chr4.4:20822080-20822099 | None:intergenic | 55.0% | |
| CTGTCCCTGGAATGGTTGGA+GGG | - | chr4.4:20821659-20821678 | MS.gene35020:intron | 55.0% | |
| GAAACAGTTGCAGCTGTCCC+TGG | - | chr4.4:20821646-20821665 | MS.gene35020:intron | 55.0% | |
| GAAGCCATCAGCGGAAGAGA+CGG | - | chr4.4:20820749-20820768 | MS.gene35020:intron | 55.0% | |
| GCATCCCTCCAACCATTCCA+GGG | + | chr4.4:20821666-20821685 | None:intergenic | 55.0% | |
| GGCGGTTGTGAGGTCATTCT+CGG | - | chr4.4:20820632-20820651 | MS.gene35020:intron | 55.0% | |
| GGGGTGAAGGAAAGTGGGAT+TGG | - | chr4.4:20822789-20822808 | MS.gene35020:intron | 55.0% | |
| GGTTGTGAGGTCATTCTCGG+CGG | - | chr4.4:20820635-20820654 | MS.gene35020:intron | 55.0% | |
| GTCAAAGTTCGGCGGTTGTG+AGG | - | chr4.4:20820622-20820641 | MS.gene35020:intron | 55.0% | |
| GTGATCCATGGGTAGGGCAA+GGG | - | chr4.4:20822675-20822694 | MS.gene35020:intron | 55.0% | |
| TCATTCTCGGCGGCGGAAAT+CGG | - | chr4.4:20820645-20820664 | MS.gene35020:intron | 55.0% | |
| TCTTCCGCTGATGGCTTCGA+CGG | + | chr4.4:20820747-20820766 | None:intergenic | 55.0% | |
| TGACACGGACACGATGACAC+CGG | + | chr4.4:20821304-20821323 | None:intergenic | 55.0% | |
| TGATCCATGGGTAGGGCAAG+GGG | - | chr4.4:20822676-20822695 | MS.gene35020:intron | 55.0% | |
| ! | ATGGCTCGCCGAGCTTTGAT+CGG | - | chr4.4:20820582-20820601 | MS.gene35020:CDS | 55.0% |
| ! | GTGGCCTTGGAACTGCTTCA+TGG | - | chr4.4:20820893-20820912 | MS.gene35020:intron | 55.0% |
| !! | GCTGGCCATTGGTTTCACAC+TGG | - | chr4.4:20823614-20823633 | MS.gene35020:CDS | 55.0% |
| AGGCATCTCTACGCCGATGG+CGG | - | chr4.4:20820672-20820691 | MS.gene35020:intron | 60.0% | |
| ATCTCCCCTTGCCCTACCCA+TGG | + | chr4.4:20822683-20822702 | None:intergenic | 60.0% | |
| CATCGGCGTAGAGATGCCTC+TGG | + | chr4.4:20820671-20820690 | None:intergenic | 60.0% | |
| CGCTGATGAGGCTCACCATC+GGG | - | chr4.4:20822062-20822081 | MS.gene35020:intron | 60.0% | |
| CGTCCCGCAGTTCTTTACCG+TGG | + | chr4.4:20823692-20823711 | None:intergenic | 60.0% | |
| GAACCCTCCGTTTCCGCCAT+CGG | + | chr4.4:20820688-20820707 | None:intergenic | 60.0% | |
| GCAGCTGTCCCTGGAATGGT+TGG | - | chr4.4:20821655-20821674 | MS.gene35020:intron | 60.0% | |
| GCTGTCCCTGGAATGGTTGG+AGG | - | chr4.4:20821658-20821677 | MS.gene35020:intron | 60.0% | |
| TCCGGCCACCGATCAAAGCT+CGG | + | chr4.4:20820593-20820612 | None:intergenic | 60.0% | |
| TCTGTCGCACGAGGCGTGTA+TGG | - | chr4.4:20821240-20821259 | MS.gene35020:intron | 60.0% | |
| TGTGAGGTCATTCTCGGCGG+CGG | - | chr4.4:20820638-20820657 | MS.gene35020:intron | 60.0% | |
| ! | GCAGGGAAGTAGTGGGGGAA+GGG | - | chr4.4:20822633-20822652 | MS.gene35020:CDS | 60.0% |
| ! | GCGCTGATGAGGCTCACCAT+CGG | - | chr4.4:20822061-20822080 | MS.gene35020:intron | 60.0% |
| ! | TGCAGGGAAGTAGTGGGGGA+AGG | - | chr4.4:20822632-20822651 | MS.gene35020:CDS | 60.0% |
| !! | CAGAGGCATCTCTACGCCGA+TGG | - | chr4.4:20820669-20820688 | MS.gene35020:intron | 60.0% |
| !! | CTCTACGCCGATGGCGGAAA+CGG | - | chr4.4:20820678-20820697 | MS.gene35020:intron | 60.0% |
| ACTCTCCCCGTCCTCCCAAG+TGG | + | chr4.4:20822588-20822607 | None:intergenic | 65.0% | |
| ATGGGTCTGGCTGCGGGTGA+AGG | - | chr4.4:20822902-20822921 | MS.gene35020:intron | 65.0% | |
| GCCATCAGCGGAAGAGACGG+AGG | - | chr4.4:20820752-20820771 | MS.gene35020:intron | 65.0% | |
| GCCTCCGTCTCTTCCGCTGA+TGG | + | chr4.4:20820756-20820775 | None:intergenic | 65.0% | |
| TGCGCCGTCGAAGCCATCAG+CGG | - | chr4.4:20820740-20820759 | MS.gene35020:intron | 65.0% | |
| TGGGTCTGGCTGCGGGTGAA+GGG | - | chr4.4:20822903-20822922 | MS.gene35020:intron | 65.0% | |
| ! | GCCGAGCTTTGATCGGTGGC+CGG | - | chr4.4:20820589-20820608 | MS.gene35020:CDS | 65.0% |
| ! | GCTAATGTGGGGGAGGCGTG+TGG | - | chr4.4:20822752-20822771 | MS.gene35020:intron | 65.0% |
| ! | GCTCGCCGAGCTTTGATCGG+TGG | - | chr4.4:20820585-20820604 | MS.gene35020:CDS | 65.0% |
| !! | ACGCCGATGGCGGAAACGGA+GGG | - | chr4.4:20820682-20820701 | MS.gene35020:intron | 65.0% |
| !! | TACGCCGATGGCGGAAACGG+AGG | - | chr4.4:20820681-20820700 | MS.gene35020:intron | 65.0% |
| CGGGACGCACCAGCTCCTCT+TGG | - | chr4.4:20823705-20823724 | MS.gene35020:CDS | 70.0% | |
| GTCTGGCTGCGGGTGAAGGG+AGG | - | chr4.4:20822906-20822925 | MS.gene35020:intron | 70.0% | |
| TGGCTGCGGGTGAAGGGAGG+AGG | - | chr4.4:20822909-20822928 | MS.gene35020:intron | 70.0% | |
| CGGCGGCGGAAATCGGCCAG+AGG | - | chr4.4:20820652-20820671 | MS.gene35020:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 20820558 | 20823737 | 20820558 | ID=MS.gene35020 |
| chr4.4 | mRNA | 20820558 | 20823737 | 20820558 | ID=MS.gene35020.t1;Parent=MS.gene35020 |
| chr4.4 | exon | 20823381 | 20823737 | 20823381 | ID=MS.gene35020.t1.exon1;Parent=MS.gene35020.t1 |
| chr4.4 | CDS | 20823381 | 20823737 | 20823381 | ID=cds.MS.gene35020.t1;Parent=MS.gene35020.t1 |
| chr4.4 | exon | 20823139 | 20823267 | 20823139 | ID=MS.gene35020.t1.exon2;Parent=MS.gene35020.t1 |
| chr4.4 | CDS | 20823139 | 20823267 | 20823139 | ID=cds.MS.gene35020.t1;Parent=MS.gene35020.t1 |
| chr4.4 | exon | 20822191 | 20822679 | 20822191 | ID=MS.gene35020.t1.exon3;Parent=MS.gene35020.t1 |
| chr4.4 | CDS | 20822191 | 20822679 | 20822191 | ID=cds.MS.gene35020.t1;Parent=MS.gene35020.t1 |
| chr4.4 | exon | 20820558 | 20820617 | 20820558 | ID=MS.gene35020.t1.exon4;Parent=MS.gene35020.t1 |
| chr4.4 | CDS | 20820558 | 20820617 | 20820558 | ID=cds.MS.gene35020.t1;Parent=MS.gene35020.t1 |
| Gene Sequence |
| Protein sequence |
