Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85071.t1 | AAQ73157.1 | 80 | 65 | 13 | 0 | 1 | 65 | 258 | 322 | 2.80E-19 | 104 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85071.t1 | A0A0P0XII1 | 52.5 | 59 | 28 | 0 | 10 | 68 | 301 | 359 | 3.2e-11 | 68.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85071.t1 | G7K7M3 | 80.0 | 65 | 13 | 0 | 1 | 65 | 298 | 362 | 2.0e-19 | 104.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050026 | MS.gene85071 | 0.813268 | 2.79E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene85071 | 0.805234 | 1.47E-49 | -1.69E-46 |
| MS.gene057598 | MS.gene85071 | 0.810847 | 9.40E-51 | -1.69E-46 |
| MS.gene058037 | MS.gene85071 | 0.804948 | 1.69E-49 | -1.69E-46 |
| MS.gene059458 | MS.gene85071 | 0.808234 | 3.42E-50 | -1.69E-46 |
| MS.gene059459 | MS.gene85071 | 0.802017 | 6.85E-49 | -1.69E-46 |
| MS.gene059460 | MS.gene85071 | 0.820036 | 8.48E-53 | -1.69E-46 |
| MS.gene059584 | MS.gene85071 | 0.815346 | 9.69E-52 | -1.69E-46 |
| MS.gene061091 | MS.gene85071 | 0.802501 | 5.44E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85071.t1 | MTR_5g086040 | 92.727 | 55 | 4 | 0 | 1 | 55 | 1 | 55 | 2.95e-28 | 103 |
| MS.gene85071.t1 | MTR_5g086040 | 92.727 | 55 | 4 | 0 | 1 | 55 | 108 | 162 | 3.91e-28 | 103 |
| MS.gene85071.t1 | MTR_5g086040 | 92.727 | 55 | 4 | 0 | 1 | 55 | 298 | 352 | 1.66e-27 | 103 |
| MS.gene85071.t1 | MTR_5g086040 | 92.727 | 55 | 4 | 0 | 1 | 55 | 260 | 314 | 1.69e-27 | 103 |
| MS.gene85071.t1 | MTR_5g086130 | 53.125 | 64 | 30 | 0 | 2 | 65 | 291 | 354 | 8.30e-18 | 75.5 |
| MS.gene85071.t1 | MTR_5g086130 | 53.125 | 64 | 30 | 0 | 2 | 65 | 289 | 352 | 8.38e-18 | 75.5 |
| MS.gene85071.t1 | MTR_5g086030 | 59.649 | 57 | 23 | 0 | 9 | 65 | 316 | 372 | 1.04e-17 | 75.5 |
| MS.gene85071.t1 | MTR_5g086310 | 60.345 | 58 | 23 | 0 | 8 | 65 | 239 | 296 | 1.41e-17 | 74.3 |
| MS.gene85071.t1 | MTR_3g080050 | 57.143 | 56 | 24 | 0 | 10 | 65 | 299 | 354 | 6.43e-17 | 73.2 |
| MS.gene85071.t1 | MTR_5g086090 | 54.237 | 59 | 27 | 0 | 7 | 65 | 280 | 338 | 4.18e-16 | 70.9 |
| MS.gene85071.t1 | MTR_5g086080 | 54.237 | 59 | 27 | 0 | 7 | 65 | 120 | 178 | 9.32e-16 | 69.7 |
| MS.gene85071.t1 | MTR_5g078590 | 63.265 | 49 | 18 | 0 | 7 | 55 | 91 | 139 | 5.85e-15 | 65.5 |
| MS.gene85071.t1 | MTR_5g086540 | 52.830 | 53 | 25 | 0 | 13 | 65 | 254 | 306 | 9.21e-14 | 64.3 |
| MS.gene85071.t1 | MTR_2g024290 | 48.333 | 60 | 31 | 0 | 6 | 65 | 291 | 350 | 3.11e-13 | 62.8 |
| MS.gene85071.t1 | MTR_2g024290 | 48.333 | 60 | 31 | 0 | 6 | 65 | 291 | 350 | 3.92e-13 | 62.4 |
| MS.gene85071.t1 | MTR_5g086120 | 50.847 | 59 | 29 | 0 | 7 | 65 | 281 | 339 | 4.76e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85071.t1 | AT3G21630 | 47.541 | 61 | 32 | 0 | 4 | 64 | 293 | 353 | 1.65e-13 | 63.5 |
Find 15 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCATGGCTAATAAAAT+TGG | 0.204108 | 4.2:-67202344 | MS.gene85071:CDS |
| AAAATTGGTGAAGATGGTTT+TGG | 0.271904 | 4.2:-67202329 | MS.gene85071:CDS |
| TACCAACATCACAAGCATTA+TGG | 0.364187 | 4.2:-67202420 | MS.gene85071:CDS |
| GCTAATAAAATTGGTGAAGA+TGG | 0.392053 | 4.2:-67202335 | MS.gene85071:CDS |
| TTCTTTGTATGAAAATTCTT+CGG | 0.414004 | 4.2:+67202388 | None:intergenic |
| AGCCATGCTGAAATTATTTG+TGG | 0.438697 | 4.2:+67202355 | None:intergenic |
| TAAGGATAAATGTGAGACTT+TGG | 0.462337 | 4.2:-67202130 | MS.gene85071:CDS |
| CACCATAATGCTTGTGATGT+TGG | 0.473095 | 4.2:+67202418 | None:intergenic |
| GCTGAAATTATTTGTGGCAA+TGG | 0.475076 | 4.2:+67202361 | None:intergenic |
| AAAGTCTCACATTTATCCTT+AGG | 0.495935 | 4.2:+67202132 | None:intergenic |
| CTATGCAGAGCTGAGAGGCC+AGG | 0.530086 | 4.2:-67202297 | MS.gene85071:intron |
| AATAGAAAGTAGGATGCCTA+AGG | 0.569248 | 4.2:-67202148 | MS.gene85071:intron |
| CAACATCACAAGCATTATGG+TGG | 0.619239 | 4.2:-67202417 | MS.gene85071:CDS |
| TGCCACAAATAATTTCAGCA+TGG | 0.643522 | 4.2:-67202357 | MS.gene85071:CDS |
| GTCTTCTATGCAGAGCTGAG+AGG | 0.646680 | 4.2:-67202302 | MS.gene85071:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCTTTGTATGAAAATTCTT+CGG | + | chr4.2:67202179-67202198 | None:intergenic | 20.0% |
| !!! | AATTTTTAAAGCATAACATG+TGG | - | chr4.2:67202386-67202405 | MS.gene85071:CDS | 20.0% |
| AAAGTCTCACATTTATCCTT+AGG | + | chr4.2:67202435-67202454 | None:intergenic | 30.0% | |
| GCTAATAAAATTGGTGAAGA+TGG | - | chr4.2:67202229-67202248 | MS.gene85071:intron | 30.0% | |
| TAAGGATAAATGTGAGACTT+TGG | - | chr4.2:67202434-67202453 | MS.gene85071:CDS | 30.0% | |
| TTCAGCATGGCTAATAAAAT+TGG | - | chr4.2:67202220-67202239 | MS.gene85071:intron | 30.0% | |
| ! | AAAATTGGTGAAGATGGTTT+TGG | - | chr4.2:67202235-67202254 | MS.gene85071:intron | 30.0% |
| !!! | TGGTTTTGTGAATAGAAAGT+AGG | - | chr4.2:67202406-67202425 | MS.gene85071:CDS | 30.0% |
| AATAGAAAGTAGGATGCCTA+AGG | - | chr4.2:67202416-67202435 | MS.gene85071:CDS | 35.0% | |
| AGCCATGCTGAAATTATTTG+TGG | + | chr4.2:67202212-67202231 | None:intergenic | 35.0% | |
| GCTGAAATTATTTGTGGCAA+TGG | + | chr4.2:67202206-67202225 | None:intergenic | 35.0% | |
| TACCAACATCACAAGCATTA+TGG | - | chr4.2:67202144-67202163 | MS.gene85071:CDS | 35.0% | |
| TGCCACAAATAATTTCAGCA+TGG | - | chr4.2:67202207-67202226 | MS.gene85071:intron | 35.0% | |
| CAACATCACAAGCATTATGG+TGG | - | chr4.2:67202147-67202166 | MS.gene85071:intron | 40.0% | |
| CACCATAATGCTTGTGATGT+TGG | + | chr4.2:67202149-67202168 | None:intergenic | 40.0% | |
| CATGCATATGATAACAGACC+TGG | + | chr4.2:67202288-67202307 | None:intergenic | 40.0% | |
| GTCTTCTATGCAGAGCTGAG+AGG | - | chr4.2:67202262-67202281 | MS.gene85071:intron | 50.0% | |
| CTATGCAGAGCTGAGAGGCC+AGG | - | chr4.2:67202267-67202286 | MS.gene85071:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 67202124 | 67202462 | 67202124 | ID=MS.gene85071 |
| chr4.2 | mRNA | 67202124 | 67202462 | 67202124 | ID=MS.gene85071.t1;Parent=MS.gene85071 |
| chr4.2 | exon | 67202298 | 67202462 | 67202298 | ID=MS.gene85071.t1.exon1;Parent=MS.gene85071.t1 |
| chr4.2 | CDS | 67202298 | 67202462 | 67202298 | ID=cds.MS.gene85071.t1;Parent=MS.gene85071.t1 |
| chr4.2 | exon | 67202124 | 67202165 | 67202124 | ID=MS.gene85071.t1.exon2;Parent=MS.gene85071.t1 |
| chr4.2 | CDS | 67202124 | 67202165 | 67202124 | ID=cds.MS.gene85071.t1;Parent=MS.gene85071.t1 |
| Gene Sequence |
| Protein sequence |
