AlfalfaGEDB | MS.gene85153

M. sativa cultivar XinJiangDaYe / MS.gene85153

KEGG: -
GO: -
PFAM: PF05553.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85153.t1	XP_003597684.1	96.9	195	6	0	1	195	1	195	6.50E-85	323.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85153.t1	G7II06	96.9	195	6	0	1	195	1	195	4.7e-85	323.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051552	MS.gene85153	0.820403	6.99E-53	-1.69E-46
MS.gene05470	MS.gene85153	-0.801922	7.16E-49	-1.69E-46
MS.gene054884	MS.gene85153	0.811573	6.54E-51	-1.69E-46
MS.gene055158	MS.gene85153	0.801027	1.09E-48	-1.69E-46
MS.gene055159	MS.gene85153	0.813367	2.65E-51	-1.69E-46
MS.gene058418	MS.gene85153	0.831271	1.84E-55	-1.69E-46
MS.gene059023	MS.gene85153	-0.806544	7.81E-50	-1.69E-46
MS.gene05914	MS.gene85153	-0.803272	3.77E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85153.t1	MTR_2g101120	96.923	195	6	0	1	195	1	195	1.10e-143	398
MS.gene85153.t1	MTR_2g101130	83.920	199	27	2	1	194	1	199	1.54e-112	319
MS.gene85153.t1	MTR_8g014030	67.857	196	54	5	1	194	1	189	1.44e-92	268
MS.gene85153.t1	MTR_8g014010	62.371	194	62	5	1	192	1	185	5.85e-80	236
MS.gene85153.t1	MTR_8g014020	64.085	142	42	5	55	194	1	135	1.72e-56	175
MS.gene85153.t1	MTR_6g088545	40.933	193	87	7	7	194	10	180	5.25e-39	132
MS.gene85153.t1	MTR_6g088525	42.487	193	83	9	7	194	10	179	2.02e-38	131
MS.gene85153.t1	MTR_6g088505	41.753	194	84	9	7	194	10	180	1.29e-36	126
MS.gene85153.t1	MTR_6g088565	37.824	193	93	6	7	194	10	180	1.28e-35	124
MS.gene85153.t1	MTR_8g102660	36.139	202	92	7	1	194	3	175	5.25e-32	114
MS.gene85153.t1	MTR_0016s0060	37.989	179	83	5	22	194	1	157	1.48e-28	105
MS.gene85153.t1	MTR_0016s0050	38.150	173	70	6	32	194	1	146	1.48e-25	97.1
MS.gene85153.t1	MTR_7g084690	34.742	213	113	13	1	195	1	205	1.07e-21	88.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85153.t1	AT1G52140	46.009	213	84	9	1	194	1	201	1.50e-51	165
MS.gene85153.t1	AT3G16330	45.327	214	79	10	1	194	1	196	9.27e-45	147
MS.gene85153.t1	AT4G29110	40.306	196	89	7	1	194	14	183	9.15e-31	111

Find 63 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTCCTGCGCTGCCTGGATT+TGG	0.199261	2.4:+3502969	MS.gene85153:CDS
TGCGGTGACGTTTCCCTATT+GGG	0.266243	2.4:-3502824	None:intergenic
CTCCTGCGCTGCCTGGATTT+GGG	0.297032	2.4:+3502970	MS.gene85153:CDS
CTGCGGTGACGTTTCCCTAT+TGG	0.315623	2.4:-3502825	None:intergenic
TGTGGTGATGGTGGAACATT+AGG	0.346556	2.4:-3502704	None:intergenic
ATCAAGCTTGCTTCCTCAAC+TGG	0.348831	2.4:-3503112	None:intergenic
CCCTCAATTGCCTTACCATT+GGG	0.354815	2.4:-3502998	None:intergenic
TTCTCATCATCTTCTCTTAG+AGG	0.359691	2.4:-3503038	None:intergenic
ATCAAAGAGAGTGTGGAATA+TGG	0.384105	2.4:+3502569	MS.gene85153:CDS
CTGCTGAAGAATTCATAAAC+AGG	0.405304	2.4:+3503081	MS.gene85153:CDS
CCCTTGATGCGGTGAATTGA+TGG	0.422995	2.4:-3502761	None:intergenic
CCCAACCACTTCTTCCCAAT+AGG	0.431411	2.4:+3502810	MS.gene85153:CDS
GAAGCATCTCCTGCGCTGCC+TGG	0.434989	2.4:+3502963	MS.gene85153:CDS
CCAACCACTTCTTCCCAATA+GGG	0.449763	2.4:+3502811	MS.gene85153:CDS
GAGCGCAATGAAGGCAGTGC+TGG	0.452696	2.4:+3502911	MS.gene85153:CDS
CCTATTGGGAAGAAGTGGTT+GGG	0.460023	2.4:-3502810	None:intergenic
AGGTGGAGCCGCCGTGGTTG+TGG	0.461991	2.4:-3502722	None:intergenic
AGCCGCCGTGGTTGTGGTGA+TGG	0.464409	2.4:-3502716	None:intergenic
GGCGTTTGAGCATCATGTTT+AGG	0.464704	2.4:-3502650	None:intergenic
ACCCTCAATTGCCTTACCAT+TGG	0.467623	2.4:-3502999	None:intergenic
GTCAAAGGGCAAACTAATGA+TGG	0.467955	2.4:+3502626	MS.gene85153:CDS
GCCTTCTTCATGTTGAGAAA+AGG	0.468789	2.4:+3502600	MS.gene85153:CDS
CGCCCAAATCCAGGCAGCGC+AGG	0.473511	2.4:-3502972	None:intergenic
GCCCAATGGTAAGGCAATTG+AGG	0.485585	2.4:+3502997	MS.gene85153:CDS
AGGTGGCGCTTGAGCGGAAG+TGG	0.487819	2.4:-3502862	None:intergenic
CACACTCTCTTTGATATCAC+TGG	0.497371	2.4:-3502561	None:intergenic
TCATCTTCTCTTAGAGGGAA+TGG	0.502641	2.4:-3503032	None:intergenic
CCCTATTGGGAAGAAGTGGT+TGG	0.503761	2.4:-3502811	None:intergenic
GGCGACAATGAGCGCAATGA+AGG	0.505967	2.4:+3502902	MS.gene85153:CDS
CCCAATGGTAAGGCAATTGA+GGG	0.507064	2.4:+3502998	MS.gene85153:CDS
AAACGCCGCAGCAAGCTCGC+CGG	0.510889	2.4:+3502666	MS.gene85153:CDS
GTGTGAGCGGCGGCTTGAGG+TGG	0.511475	2.4:-3502739	None:intergenic
ATTGGGCTACGCCCAAATCC+AGG	0.513644	2.4:-3502981	None:intergenic
GAAGGACGATCAAGTTGATA+AGG	0.519057	2.4:+3503058	MS.gene85153:CDS
CGCTCAAGCGCCACCTACAC+AGG	0.523001	2.4:+3502869	MS.gene85153:CDS
TCTCATCATCTTCTCTTAGA+GGG	0.530370	2.4:-3503037	None:intergenic
TTGAGAAAAGGCATGTCAAA+GGG	0.534014	2.4:+3502612	MS.gene85153:CDS
ACCATCAATTCACCGCATCA+AGG	0.537653	2.4:+3502760	MS.gene85153:CDS
CTGTGTAGGTGGCGCTTGAG+CGG	0.542519	2.4:-3502868	None:intergenic
GTTGAGAAAAGGCATGTCAA+AGG	0.559027	2.4:+3502611	MS.gene85153:CDS
ACCTCATCATCCTGTGTAGG+TGG	0.565659	2.4:-3502879	None:intergenic
GTGGAACATTAGGTTGGCAA+TGG	0.565737	2.4:-3502694	None:intergenic
GTTTCCCTATTGGGAAGAAG+TGG	0.569833	2.4:-3502815	None:intergenic
TGGAGAAGTTGTTGTGGCTG+CGG	0.571576	2.4:-3502842	None:intergenic
TTGGGCGTAGCCCAATGGTA+AGG	0.575007	2.4:+3502988	MS.gene85153:CDS
CGCCGTGGTTGTGGTGATGG+TGG	0.578705	2.4:-3502713	None:intergenic
TGGATTTGGGCGTAGCCCAA+TGG	0.585672	2.4:+3502983	MS.gene85153:CDS
TCAAAGGGCAAACTAATGAT+GGG	0.588410	2.4:+3502627	MS.gene85153:CDS
CAGTGATATCAAAGAGAGTG+TGG	0.593721	2.4:+3502562	MS.gene85153:CDS
ATGGTGTGAGCGGCGGCTTG+AGG	0.594186	2.4:-3502742	None:intergenic
TCTAAGAGAAGATGATGAGA+AGG	0.594411	2.4:+3503040	MS.gene85153:CDS
TGAATTGATGGTGTGAGCGG+CGG	0.628937	2.4:-3502749	None:intergenic
ACCTACACAGGATGATGAGG+TGG	0.634579	2.4:+3502881	MS.gene85153:CDS
CCATCAATTCACCGCATCAA+GGG	0.637439	2.4:+3502761	MS.gene85153:CDS
GGCTTGAGGTGGAGCCGCCG+TGG	0.640629	2.4:-3502728	None:intergenic
TTCCACCATCACCACAACCA+CGG	0.649090	2.4:+3502711	MS.gene85153:CDS
GCCACCTCATCATCCTGTGT+AGG	0.650227	2.4:-3502882	None:intergenic
CGGTGAATTGATGGTGTGAG+CGG	0.651040	2.4:-3502752	None:intergenic
CGGAAGTGGAGAAGTTGTTG+TGG	0.651141	2.4:-3502848	None:intergenic
GTGATGGTGGAACATTAGGT+TGG	0.654305	2.4:-3502700	None:intergenic
GCCACCTACACAGGATGATG+AGG	0.661418	2.4:+3502878	MS.gene85153:CDS
GAATTCATACTCCCTTGATG+CGG	0.696823	2.4:-3502772	None:intergenic
CACCATCACCACAACCACGG+CGG	0.721311	2.4:+3502714	MS.gene85153:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTGCTGAAGAATTCATAAAC+AGG	+	chr2.4:3503081-3503100	MS.gene85153:CDS	35.0%
	TCAAAGGGCAAACTAATGAT+GGG	+	chr2.4:3502627-3502646	MS.gene85153:CDS	35.0%
	TCTAAGAGAAGATGATGAGA+AGG	+	chr2.4:3503040-3503059	MS.gene85153:CDS	35.0%
	TCTCATCATCTTCTCTTAGA+GGG	-	chr2.4:3503040-3503059	None:intergenic	35.0%
	TTCTCATCATCTTCTCTTAG+AGG	-	chr2.4:3503041-3503060	None:intergenic	35.0%
!	ATCAAAGAGAGTGTGGAATA+TGG	+	chr2.4:3502569-3502588	MS.gene85153:CDS	35.0%
!	TTGAGAAAAGGCATGTCAAA+GGG	+	chr2.4:3502612-3502631	MS.gene85153:CDS	35.0%
	CACACTCTCTTTGATATCAC+TGG	-	chr2.4:3502564-3502583	None:intergenic	40.0%
	CAGTGATATCAAAGAGAGTG+TGG	+	chr2.4:3502562-3502581	MS.gene85153:CDS	40.0%
	GAATTCATACTCCCTTGATG+CGG	-	chr2.4:3502775-3502794	None:intergenic	40.0%
	GCCTTCTTCATGTTGAGAAA+AGG	+	chr2.4:3502600-3502619	MS.gene85153:CDS	40.0%
	GTCAAAGGGCAAACTAATGA+TGG	+	chr2.4:3502626-3502645	MS.gene85153:CDS	40.0%
	TCATCTTCTCTTAGAGGGAA+TGG	-	chr2.4:3503035-3503054	None:intergenic	40.0%
!	GCCTTTTCTCAACATGAAGA+AGG	-	chr2.4:3502604-3502623	None:intergenic	40.0%
!	GTTGAGAAAAGGCATGTCAA+AGG	+	chr2.4:3502611-3502630	MS.gene85153:CDS	40.0%
!!	GAAGGACGATCAAGTTGATA+AGG	+	chr2.4:3503058-3503077	MS.gene85153:CDS	40.0%
	ACCATCAATTCACCGCATCA+AGG	+	chr2.4:3502760-3502779	MS.gene85153:CDS	45.0%
	ACCCTCAATTGCCTTACCAT+TGG	-	chr2.4:3503002-3503021	None:intergenic	45.0%
	CCAACCACTTCTTCCCAATA+GGG	+	chr2.4:3502811-3502830	MS.gene85153:CDS	45.0%
	CCATCAATTCACCGCATCAA+GGG	+	chr2.4:3502761-3502780	MS.gene85153:CDS	45.0%
	CCCAATGGTAAGGCAATTGA+GGG	+	chr2.4:3502998-3503017	MS.gene85153:CDS	45.0%
	CCCTCAATTGCCTTACCATT+GGG	-	chr2.4:3503001-3503020	None:intergenic	45.0%
	CCTATTGGGAAGAAGTGGTT+GGG	-	chr2.4:3502813-3502832	None:intergenic	45.0%
	GGCGTTTGAGCATCATGTTT+AGG	-	chr2.4:3502653-3502672	None:intergenic	45.0%
	GTTTCCCTATTGGGAAGAAG+TGG	-	chr2.4:3502818-3502837	None:intergenic	45.0%
!	ACAGGTTTTACAGCCAGTTG+AGG	+	chr2.4:3503099-3503118	MS.gene85153:CDS	45.0%
!!	GTGATGGTGGAACATTAGGT+TGG	-	chr2.4:3502703-3502722	None:intergenic	45.0%
!!	GTGGAACATTAGGTTGGCAA+TGG	-	chr2.4:3502697-3502716	None:intergenic	45.0%
!!	TAGGTTGGCAATGGCTTTTC+CGG	-	chr2.4:3502688-3502707	None:intergenic	45.0%
!!	TGTGGTGATGGTGGAACATT+AGG	-	chr2.4:3502707-3502726	None:intergenic	45.0%
	ACCTACACAGGATGATGAGG+TGG	+	chr2.4:3502881-3502900	MS.gene85153:CDS	50.0%
	ACCTCATCATCCTGTGTAGG+TGG	-	chr2.4:3502882-3502901	None:intergenic	50.0%
	CCCAACCACTTCTTCCCAAT+AGG	+	chr2.4:3502810-3502829	MS.gene85153:CDS	50.0%
	CCCTATTGGGAAGAAGTGGT+TGG	-	chr2.4:3502814-3502833	None:intergenic	50.0%
	CCCTTGATGCGGTGAATTGA+TGG	-	chr2.4:3502764-3502783	None:intergenic	50.0%
	CGGAAGTGGAGAAGTTGTTG+TGG	-	chr2.4:3502851-3502870	None:intergenic	50.0%
	GCCCAATGGTAAGGCAATTG+AGG	+	chr2.4:3502997-3503016	MS.gene85153:CDS	50.0%
	TGCGGTGACGTTTCCCTATT+GGG	-	chr2.4:3502827-3502846	None:intergenic	50.0%
	TGGAGAAGTTGTTGTGGCTG+CGG	-	chr2.4:3502845-3502864	None:intergenic	50.0%
	TTCCACCATCACCACAACCA+CGG	+	chr2.4:3502711-3502730	MS.gene85153:CDS	50.0%
!!	CGGTGAATTGATGGTGTGAG+CGG	-	chr2.4:3502755-3502774	None:intergenic	50.0%
!!	TGAATTGATGGTGTGAGCGG+CGG	-	chr2.4:3502752-3502771	None:intergenic	50.0%
	ATTGGGCTACGCCCAAATCC+AGG	-	chr2.4:3502984-3503003	None:intergenic	55.0%
	CTGCGGTGACGTTTCCCTAT+TGG	-	chr2.4:3502828-3502847	None:intergenic	55.0%
	GCCACCTACACAGGATGATG+AGG	+	chr2.4:3502878-3502897	MS.gene85153:CDS	55.0%
	GCCACCTCATCATCCTGTGT+AGG	-	chr2.4:3502885-3502904	None:intergenic	55.0%
	GGCGACAATGAGCGCAATGA+AGG	+	chr2.4:3502902-3502921	MS.gene85153:CDS	55.0%
	TGGATTTGGGCGTAGCCCAA+TGG	+	chr2.4:3502983-3503002	MS.gene85153:CDS	55.0%
	TTGGGCGTAGCCCAATGGTA+AGG	+	chr2.4:3502988-3503007	MS.gene85153:CDS	55.0%
	CACCATCACCACAACCACGG+CGG	+	chr2.4:3502714-3502733	MS.gene85153:CDS	60.0%
	CTCCTGCGCTGCCTGGATTT+GGG	+	chr2.4:3502970-3502989	MS.gene85153:CDS	60.0%
	CTGTGTAGGTGGCGCTTGAG+CGG	-	chr2.4:3502871-3502890	None:intergenic	60.0%
	GAGCGCAATGAAGGCAGTGC+TGG	+	chr2.4:3502911-3502930	MS.gene85153:CDS	60.0%
	TCTCCTGCGCTGCCTGGATT+TGG	+	chr2.4:3502969-3502988	MS.gene85153:CDS	60.0%
!!!	CTTTTCCGGCGAGCTTGCTG+CGG	-	chr2.4:3502674-3502693	None:intergenic	60.0%
	AAACGCCGCAGCAAGCTCGC+CGG	+	chr2.4:3502666-3502685	MS.gene85153:CDS	65.0%
	AGCCGCCGTGGTTGTGGTGA+TGG	-	chr2.4:3502719-3502738	None:intergenic	65.0%
	AGGTGGCGCTTGAGCGGAAG+TGG	-	chr2.4:3502865-3502884	None:intergenic	65.0%
	ATGGTGTGAGCGGCGGCTTG+AGG	-	chr2.4:3502745-3502764	None:intergenic	65.0%
	CGCTCAAGCGCCACCTACAC+AGG	+	chr2.4:3502869-3502888	MS.gene85153:CDS	65.0%
!	GAAGCATCTCCTGCGCTGCC+TGG	+	chr2.4:3502963-3502982	MS.gene85153:CDS	65.0%
!!	CGCCGTGGTTGTGGTGATGG+TGG	-	chr2.4:3502716-3502735	None:intergenic	65.0%
	AGGTGGAGCCGCCGTGGTTG+TGG	-	chr2.4:3502725-3502744	None:intergenic	70.0%
	CGCCCAAATCCAGGCAGCGC+AGG	-	chr2.4:3502975-3502994	None:intergenic	70.0%
	GTGTGAGCGGCGGCTTGAGG+TGG	-	chr2.4:3502742-3502761	None:intergenic	70.0%
	GGCTTGAGGTGGAGCCGCCG+TGG	-	chr2.4:3502731-3502750	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	3502546	3503133	3502546	ID=MS.gene85153
chr2.4	mRNA	3502546	3503133	3502546	ID=MS.gene85153.t1;Parent=MS.gene85153
chr2.4	exon	3502546	3503133	3502546	ID=MS.gene85153.t1.exon1;Parent=MS.gene85153.t1
chr2.4	CDS	3502546	3503133	3502546	ID=cds.MS.gene85153.t1;Parent=MS.gene85153.t1

Gene Sequence

>MS.gene85153

ATGGAAAGCAATCTACCAGTGATATCAAAGAGAGTGTGGAATATGGTACGTGTAGCCTTCTTCATGTTGAGAAAAGGCATGTCAAAGGGCAAACTAATGATGGGCCTAAACATGATGCTCAAACGCCGCAGCAAGCTCGCCGGAAAAGCCATTGCCAACCTAATGTTCCACCATCACCACAACCACGGCGGCTCCACCTCAAGCCGCCGCTCACACCATCAATTCACCGCATCAAGGGAGTATGAATTCAGCTGTAGCAACACTCCCAACCACTTCTTCCCAATAGGGAAACGTCACCGCAGCCACAACAACTTCTCCACTTCCGCTCAAGCGCCACCTACACAGGATGATGAGGTGGCGACAATGAGCGCAATGAAGGCAGTGCTGGAGATGCTTAGCAATGATCAATCGATTGTAGAAGCATCTCCTGCGCTGCCTGGATTTGGGCGTAGCCCAATGGTAAGGCAATTGAGGGTGACTGACTCACCATTCCCTCTAAGAGAAGATGATGAGAAGGACGATCAAGTTGATAAGGCTGCTGAAGAATTCATAAACAGGTTTTACAGCCAGTTGAGGAAGCAAGCTTGA

Protein sequence

>MS.gene85153.t1

MESNLPVISKRVWNMVRVAFFMLRKGMSKGKLMMGLNMMLKRRSKLAGKAIANLMFHHHHNHGGSTSSRRSHHQFTASREYEFSCSNTPNHFFPIGKRHRSHNNFSTSAQAPPTQDDEVATMSAMKAVLEMLSNDQSIVEASPALPGFGRSPMVRQLRVTDSPFPLREDDEKDDQVDKAAEEFINRFYSQLRKQA