AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85201

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85201.t1 MTR_2g100240 99.293 283 2 0 1 283 1 283 0.0 575
MS.gene85201.t1 MTR_2g060900 36.842 285 138 5 13 255 1 285 8.90e-49 164
MS.gene85201.t1 MTR_7g019180 36.859 312 162 9 1 281 1 308 5.51e-47 162
MS.gene85201.t1 MTR_4g129730 35.135 222 144 0 32 253 1 222 1.98e-40 141
MS.gene85201.t1 MTR_4g129730 36.036 222 131 2 32 253 1 211 6.62e-40 139
MS.gene85201.t1 MTR_5g072760 31.224 237 152 5 44 270 37 272 4.81e-35 128
MS.gene85201.t1 MTR_5g072760 31.364 220 142 4 44 255 37 255 1.70e-33 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85201.t1 AT1G55170 51.399 286 131 4 1 282 1 282 8.80e-87 261
MS.gene85201.t1 AT3G14750 38.696 230 131 3 37 257 63 291 2.10e-46 159
MS.gene85201.t1 AT1G67170 38.647 207 127 0 33 239 47 253 5.86e-42 148
MS.gene85201.t1 AT1G67170 38.647 207 127 0 33 239 47 253 5.86e-42 148
MS.gene85201.t1 AT2G30120 26.957 230 154 1 42 257 37 266 4.38e-21 90.9
MS.gene85201.t1 AT2G30120 26.754 228 153 1 42 255 37 264 5.98e-21 90.1
MS.gene85201.t1 AT2G30120 26.638 229 154 1 41 255 36 264 7.05e-21 90.1
MS.gene85201.t1 AT5G61920 28.351 194 139 0 28 221 37 230 1.69e-18 82.8
MS.gene85201.t1 AT5G61920 28.351 194 139 0 28 221 37 230 1.69e-18 82.8
MS.gene85201.t1 AT5G61920 28.351 194 139 0 28 221 37 230 1.69e-18 82.8
MS.gene85201.t1 AT5G61920 28.351 194 139 0 28 221 37 230 1.69e-18 82.8
MS.gene85201.t1 AT5G61920 28.351 194 139 0 28 221 37 230 1.69e-18 82.8
MS.gene85201.t1 AT5G61920 28.902 173 123 0 49 221 17 189 1.44e-16 76.6
MS.gene85201.t1 AT2G30120 27.972 143 103 0 39 181 34 176 9.64e-12 62.8
MS.gene85201.t1 AT2G30120 27.972 143 103 0 39 181 34 176 1.67e-11 62.0
MS.gene85201.t1 AT2G30120 27.972 143 103 0 39 181 34 176 1.67e-11 62.0

Find 80 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGAGAAAGGTCCTCTTTA+TGG 0.176094 2.4:-4131860 MS.gene85201:CDS
TTGCCTTATGGAACAAATTT+TGG 0.192469 2.4:-4132029 MS.gene85201:CDS
ATTCATGCAGGTTTGCCTTA+TGG 0.235597 2.4:-4132041 MS.gene85201:intron
ATGGGGTGGGTACTGCTTCT+AGG 0.246552 2.4:-4131841 MS.gene85201:CDS
CACTTACGAAAGATGTTTCT+AGG 0.253529 2.4:-4133103 MS.gene85201:CDS
AGTATCAAACAAGAACTTTC+TGG 0.261139 2.4:-4133137 MS.gene85201:CDS
CACGAAGTTTCTCAACTTCT+CGG 0.317894 2.4:+4132806 None:intergenic
ACCTAGCGCGCATCAGTTCT+TGG 0.323755 2.4:+4133024 None:intergenic
ACCTCATCCAGCATTGTTAG+AGG 0.338446 2.4:-4133432 MS.gene85201:CDS
CCTCCATATGCAGATGGATA+TGG 0.340583 2.4:-4131984 MS.gene85201:CDS
CAGAGAAAGGTCCTCTTTAT+GGG 0.349670 2.4:-4131859 MS.gene85201:CDS
TCAATGAGATCTGAGATTGA+TGG 0.370744 2.4:-4133053 MS.gene85201:CDS
GGAGGCTGGTGGCGGATAAC+AGG 0.382841 2.4:-4133379 MS.gene85201:CDS
CTTCCGATGGATACCCTCTT+CGG 0.387777 2.4:+4133501 None:intergenic
GAAGGCAAACATGGAGTTCA+TGG 0.395776 2.4:-4132869 MS.gene85201:CDS
CTACCAAAATTTGTTCCATA+AGG 0.410355 2.4:+4132026 None:intergenic
TGAAGTTGGAGTCTGATCTT+AGG 0.426762 2.4:-4133220 MS.gene85201:CDS
GGTGGTGGCCCGCGAGCATA+AGG 0.435456 2.4:+4133476 None:intergenic
TAGCATGGATGGAAGACATT+GGG 0.438242 2.4:-4132773 MS.gene85201:CDS
TTTCTCAACTTCTCGGGCCA+TGG 0.443621 2.4:+4132813 None:intergenic
TTCCGATGGATACCCTCTTC+GGG 0.447941 2.4:+4133502 None:intergenic
AAACATCTTTCGTAAGTGTC+TGG 0.450505 2.4:+4133108 None:intergenic
GTTTAGGCTGCTGCAGAGAA+AGG 0.464656 2.4:-4131872 MS.gene85201:intron
GATGGAAGACATTGGGGAGC+TGG 0.465511 2.4:-4132766 MS.gene85201:intron
GTATCAAACAAGAACTTTCT+GGG 0.473347 2.4:-4133136 MS.gene85201:CDS
TCAATCTCAGATCTCATTGA+AGG 0.475047 2.4:+4133056 None:intergenic
ACGAAGTTTCTCAACTTCTC+GGG 0.483936 2.4:+4132807 None:intergenic
AGCTCTTCCTCTAACAATGC+TGG 0.487736 2.4:+4133425 None:intergenic
ACACCATATCCATCTGCATA+TGG 0.495250 2.4:+4131981 None:intergenic
GCGAGACCTTGCAGCTGCGA+AGG 0.508426 2.4:-4133327 MS.gene85201:CDS
TATGGTGGATTATGAAAAGA+AGG 0.509898 2.4:-4132887 MS.gene85201:CDS
TACCCTCTTCGGGAACTCAT+TGG 0.513298 2.4:+4133512 None:intergenic
TCCTCTAACAATGCTGGATG+AGG 0.514770 2.4:+4133431 None:intergenic
TGCTGTCGTGGCATTGGTGG+TGG 0.516987 2.4:+4133461 None:intergenic
TATCCATCTGCATATGGAGG+TGG 0.517988 2.4:+4131987 None:intergenic
GCTGAGCTTGCTAGCATGGA+TGG 0.521001 2.4:-4132784 MS.gene85201:CDS
TCGTGCTGAGCTTGCTAGCA+TGG 0.521988 2.4:-4132788 MS.gene85201:CDS
CTGAACATGAGATGCATTCG+AGG 0.524074 2.4:-4133262 MS.gene85201:CDS
CATCGTATGAATTTAGCGAT+TGG 0.524610 2.4:-4133296 MS.gene85201:CDS
GCAGTACCCACCCCATAAAG+AGG 0.528840 2.4:+4131848 None:intergenic
AGACCTTGCAGCTGCGAAGG+AGG 0.529458 2.4:-4133324 MS.gene85201:CDS
CATGGAACAGAGGCAGTCAA+TGG 0.533149 2.4:-4132851 MS.gene85201:CDS
TGTATAACTTCATTCTTCAA+CGG 0.535567 2.4:+4133188 None:intergenic
GGAATATGCTGTCGTGGCAT+TGG 0.548596 2.4:+4133455 None:intergenic
TCTAACAATGCTGGATGAGG+TGG 0.550555 2.4:+4133434 None:intergenic
CCTCTTCGGGAACTCATTGG+TGG 0.551456 2.4:+4133515 None:intergenic
CTAGCATGGATGGAAGACAT+TGG 0.560779 2.4:-4132774 MS.gene85201:CDS
TCGGAAGGTCCTTATGCTCG+CGG 0.566608 2.4:-4133485 MS.gene85201:CDS
ACCAAGAACTGATGCGCGCT+AGG 0.570107 2.4:-4133025 MS.gene85201:intron
TTATGAAAAGAAGGCAAACA+TGG 0.573229 2.4:-4132878 MS.gene85201:CDS
GCTCCTCCTTCGCAGCTGCA+AGG 0.574836 2.4:+4133321 None:intergenic
ATTCAACGACGGTTTGAGCG+AGG 0.579107 2.4:+4131803 None:intergenic
AAAGGTCCTCTTTATGGGGT+GGG 0.581180 2.4:-4131854 MS.gene85201:CDS
GAAAGGTCCTCTTTATGGGG+TGG 0.586390 2.4:-4131855 MS.gene85201:CDS
ATATGCTGTCGTGGCATTGG+TGG 0.591214 2.4:+4133458 None:intergenic
ACAGGAGGTTGATTGATGAT+CGG 0.594043 2.4:-4133361 MS.gene85201:CDS
TTCCCACCTCCATATGCAGA+TGG 0.594536 2.4:-4131990 MS.gene85201:CDS
ATCCATCTGCATATGGAGGT+GGG 0.596363 2.4:+4131988 None:intergenic
CGGAAGGTCCTTATGCTCGC+GGG 0.597736 2.4:-4133484 MS.gene85201:CDS
CAAACAAGAACTTTCTGGGA+AGG 0.599275 2.4:-4133132 MS.gene85201:CDS
TGAAATGAGGAGGCTGGTGG+CGG 0.604523 2.4:-4133387 MS.gene85201:CDS
GCATGCTGAAATGAGGAGGC+TGG 0.605896 2.4:-4133393 MS.gene85201:CDS
GGCTGGTGGCGGATAACAGG+AGG 0.608308 2.4:-4133376 MS.gene85201:CDS
CACCAATGAGTTCCCGAAGA+GGG 0.614590 2.4:-4133514 MS.gene85201:CDS
CGAGCATAAGGACCTTCCGA+TGG 0.617038 2.4:+4133488 None:intergenic
CGAAGAGGGTATCCATCGGA+AGG 0.623087 2.4:-4133500 MS.gene85201:CDS
AAGTGCAGCATGCTGAAATG+AGG 0.630681 2.4:-4133400 MS.gene85201:CDS
TTCCCGAAGAGGGTATCCAT+CGG 0.640372 2.4:-4133504 MS.gene85201:CDS
AGATGGATATGGTGTTCACT+TGG 0.643737 2.4:-4131973 MS.gene85201:intron
AGCATGGATGGAAGACATTG+GGG 0.653311 2.4:-4132772 MS.gene85201:CDS
TGAGGTGGAATATGCTGTCG+TGG 0.657020 2.4:+4133449 None:intergenic
CCATATCCATCTGCATATGG+AGG 0.657742 2.4:+4131984 None:intergenic
TGCTGAAATGAGGAGGCTGG+TGG 0.673111 2.4:-4133390 MS.gene85201:CDS
TGAACATGAGATGCATTCGA+GGG 0.676175 2.4:-4133261 MS.gene85201:CDS
AGAGAAAGGTCCTCTTTATG+GGG 0.677550 2.4:-4131858 MS.gene85201:CDS
TGCAGCATGCTGAAATGAGG+AGG 0.680683 2.4:-4133397 MS.gene85201:CDS
CAATCACTTAGATTCAACGA+CGG 0.682596 2.4:+4131792 None:intergenic
TTGGTGGCTCTCTATGAACA+CGG 0.684269 2.4:+4133531 None:intergenic
ACATGGAGTTCATGGAACAG+AGG 0.685058 2.4:-4132861 MS.gene85201:CDS
CCACCAATGAGTTCCCGAAG+AGG 0.693852 2.4:-4133515 MS.gene85201:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATAAAAGACATGAAAAA+CGG - chr2.4:4132762-4132781 MS.gene85201:intron 15.0%
!!! ATTTTTGTTTTATAGAACTA+TGG - chr2.4:4132445-4132464 MS.gene85201:intron 15.0%
!! AATGAAATGACATGAATAAT+AGG - chr2.4:4132731-4132750 MS.gene85201:intron 20.0%
!! ATCCAATTAACACAAATAAT+AGG + chr2.4:4133159-4133178 None:intergenic 20.0%
!! ATCCTATTATTTGTGTTAAT+TGG - chr2.4:4133154-4133173 MS.gene85201:CDS 20.0%
!!! TGTTGATAAAAATATGAAGT+TGG - chr2.4:4132116-4132135 MS.gene85201:intron 20.0%
!!! TTTTGTGTATTATGTGATTA+CGG + chr2.4:4132861-4132880 None:intergenic 20.0%
!!! TTTTTAGAATTTAAGGCAAT+TGG - chr2.4:4132889-4132908 MS.gene85201:CDS 20.0%
! AATCACATAATACACAAAAG+TGG - chr2.4:4132862-4132881 MS.gene85201:CDS 25.0%
! AATGAAGCAAACGTTAAAAA+AGG + chr2.4:4133292-4133311 None:intergenic 25.0%
! ATCTTTCAGACAATTATGAA+GGG + chr2.4:4132820-4132839 None:intergenic 25.0%
! ATGAAGCAAACGTTAAAAAA+GGG + chr2.4:4133291-4133310 None:intergenic 25.0%
! TGTATAACTTCATTCTTCAA+CGG + chr2.4:4132165-4132184 None:intergenic 25.0%
!! GGTCTAAAATTATTGTGTTA+AGG + chr2.4:4133413-4133432 None:intergenic 25.0%
!!! AGAATTTTAGTATTCATCGA+GGG - chr2.4:4133008-4133027 MS.gene85201:intron 25.0%
!!! TGGCAGTTTTTTAGAATTTA+AGG - chr2.4:4132882-4132901 MS.gene85201:CDS 25.0%
!!! TTTGTTTTATAGAACTATGG+TGG - chr2.4:4132448-4132467 MS.gene85201:intron 25.0%
AAGCAAAGTAAATGAAACCT+TGG + chr2.4:4133450-4133469 None:intergenic 30.0%
AAGGATGATAAATTCTGCAA+AGG + chr2.4:4132632-4132651 None:intergenic 30.0%
AGTATCAAACAAGAACTTTC+TGG - chr2.4:4132213-4132232 MS.gene85201:intron 30.0%
ATAATTGTCTGAAAGATGGT+TGG - chr2.4:4132823-4132842 MS.gene85201:CDS 30.0%
CATCTTTCAGACAATTATGA+AGG + chr2.4:4132821-4132840 None:intergenic 30.0%
CTTCATAATTGTCTGAAAGA+TGG - chr2.4:4132819-4132838 MS.gene85201:CDS 30.0%
GAGTTAAGGTGATTAAGAAT+AGG + chr2.4:4132944-4132963 None:intergenic 30.0%
GCTATGCTAGTAAATACATA+TGG + chr2.4:4132667-4132686 None:intergenic 30.0%
GTATCAAACAAGAACTTTCT+GGG - chr2.4:4132214-4132233 MS.gene85201:intron 30.0%
TAATTGTCTGAAAGATGGTT+GGG - chr2.4:4132824-4132843 MS.gene85201:CDS 30.0%
TATAGACACATCATGATGTT+TGG - chr2.4:4133189-4133208 MS.gene85201:CDS 30.0%
TGAAGCAAACGTTAAAAAAG+GGG + chr2.4:4133290-4133309 None:intergenic 30.0%
TTATGAAAAGAAGGCAAACA+TGG - chr2.4:4132472-4132491 MS.gene85201:intron 30.0%
! ATATCTTTCGATCTTTTGAC+TGG - chr2.4:4133061-4133080 MS.gene85201:CDS 30.0%
! CTACCAAAATTTGTTCCATA+AGG + chr2.4:4133327-4133346 None:intergenic 30.0%
! TCTATCTTTAAGCATCCTTT+TGG - chr2.4:4132381-4132400 MS.gene85201:intron 30.0%
! TTGCCTTATGGAACAAATTT+TGG - chr2.4:4133321-4133340 MS.gene85201:CDS 30.0%
!! CAGAATTTTAGTATTCATCG+AGG - chr2.4:4133007-4133026 MS.gene85201:intron 30.0%
!! CTATGTGATGATTTTGAGAA+CGG - chr2.4:4132984-4133003 MS.gene85201:intron 30.0%
!! TATGGTGGATTATGAAAAGA+AGG - chr2.4:4132463-4132482 MS.gene85201:intron 30.0%
AAACATCTTTCGTAAGTGTC+TGG + chr2.4:4132245-4132264 None:intergenic 35.0%
AATTGTCTGAAAGATGGTTG+GGG - chr2.4:4132825-4132844 MS.gene85201:CDS 35.0%
ACCTTGGAAAATTGAATACG+AGG + chr2.4:4133434-4133453 None:intergenic 35.0%
CACTTACGAAAGATGTTTCT+AGG - chr2.4:4132247-4132266 MS.gene85201:intron 35.0%
CATCGTATGAATTTAGCGAT+TGG - chr2.4:4132054-4132073 MS.gene85201:intron 35.0%
GGAGTTCATTAGTGATAACA+GGG - chr2.4:4133029-4133048 MS.gene85201:CDS 35.0%
TCAATCTCAGATCTCATTGA+AGG + chr2.4:4132297-4132316 None:intergenic 35.0%
! ACCTCGTATTCAATTTTCCA+AGG - chr2.4:4133430-4133449 MS.gene85201:CDS 35.0%
! TCAATGAGATCTGAGATTGA+TGG - chr2.4:4132297-4132316 MS.gene85201:intron 35.0%
! TCTTTAAGCATCCTTTTGGT+TGG - chr2.4:4132385-4132404 MS.gene85201:intron 35.0%
! TGCTACAGTATCTGCATTTT+TGG + chr2.4:4133135-4133154 None:intergenic 35.0%
! TTGTGGATTGATTGCTAACA+CGG - chr2.4:4132349-4132368 MS.gene85201:intron 35.0%
!! AACAAATTTTGGTAGTCCAG+AGG - chr2.4:4133332-4133351 MS.gene85201:CDS 35.0%
!! ACAAATTTTGGTAGTCCAGA+GGG - chr2.4:4133333-4133352 MS.gene85201:CDS 35.0%
!! CTTTAAGCATCCTTTTGGTT+GGG - chr2.4:4132386-4132405 MS.gene85201:intron 35.0%
AACGTTTGCTTCATTCATGC+AGG - chr2.4:4133297-4133316 MS.gene85201:CDS 40.0%
ACACCATATCCATCTGCATA+TGG + chr2.4:4133372-4133391 None:intergenic 40.0%
ACGAAGTTTCTCAACTTCTC+GGG + chr2.4:4132546-4132565 None:intergenic 40.0%
AGAGAAAGGTCCTCTTTATG+GGG - chr2.4:4133492-4133511 MS.gene85201:CDS 40.0%
AGATGGATATGGTGTTCACT+TGG - chr2.4:4133377-4133396 MS.gene85201:CDS 40.0%
ATGCAGAAATCCCAACCAAA+AGG + chr2.4:4132399-4132418 None:intergenic 40.0%
ATTCATGCAGGTTTGCCTTA+TGG - chr2.4:4133309-4133328 MS.gene85201:CDS 40.0%
ATTGTCTGAAAGATGGTTGG+GGG - chr2.4:4132826-4132845 MS.gene85201:CDS 40.0%
CACGAAGTTTCTCAACTTCT+CGG + chr2.4:4132547-4132566 None:intergenic 40.0%
CAGAGAAAGGTCCTCTTTAT+GGG - chr2.4:4133491-4133510 MS.gene85201:CDS 40.0%
CATATGGAGTTAACGACTGA+AGG + chr2.4:4132651-4132670 None:intergenic 40.0%
CTTTGCTTGTGCTTCTGTTT+AGG - chr2.4:4133462-4133481 MS.gene85201:CDS 40.0%
GCAGTCAATGGAGAAAAACA+TGG - chr2.4:4132511-4132530 MS.gene85201:intron 40.0%
GGAGAAAAACATGGTTTCCA+TGG - chr2.4:4132520-4132539 MS.gene85201:intron 40.0%
GGGAGTTCATTAGTGATAAC+AGG - chr2.4:4133028-4133047 MS.gene85201:CDS 40.0%
TAGACAAGTTAGAGAAAGCG+TGG + chr2.4:4132967-4132986 None:intergenic 40.0%
TAGCATGGATGGAAGACATT+GGG - chr2.4:4132577-4132596 MS.gene85201:intron 40.0%
TGAACATGAGATGCATTCGA+GGG - chr2.4:4132089-4132108 MS.gene85201:intron 40.0%
TGAAGTTGGAGTCTGATCTT+AGG - chr2.4:4132130-4132149 MS.gene85201:intron 40.0%
! ACAGGAGGTTGATTGATGAT+CGG - chr2.4:4131989-4132008 MS.gene85201:CDS 40.0%
! AGACAAGTTAGAGAAAGCGT+GGG + chr2.4:4132966-4132985 None:intergenic 40.0%
! CAAACAAGAACTTTCTGGGA+AGG - chr2.4:4132218-4132237 MS.gene85201:intron 40.0%
!! AAATTTTGGTAGTCCAGAGG+GGG - chr2.4:4133335-4133354 MS.gene85201:CDS 40.0%
!! CAAATTTTGGTAGTCCAGAG+GGG - chr2.4:4133334-4133353 MS.gene85201:CDS 40.0%
AAAGGTCCTCTTTATGGGGT+GGG - chr2.4:4133496-4133515 MS.gene85201:CDS 45.0%
AAGTGCAGCATGCTGAAATG+AGG - chr2.4:4131950-4131969 MS.gene85201:intron 45.0%
ACATGGAGTTCATGGAACAG+AGG - chr2.4:4132489-4132508 MS.gene85201:intron 45.0%
ACCTCATCCAGCATTGTTAG+AGG - chr2.4:4131918-4131937 MS.gene85201:intron 45.0%
AGCATGGATGGAAGACATTG+GGG - chr2.4:4132578-4132597 MS.gene85201:intron 45.0%
AGCTCTTCCTCTAACAATGC+TGG + chr2.4:4131928-4131947 None:intergenic 45.0%
ATCCATCTGCATATGGAGGT+GGG + chr2.4:4133365-4133384 None:intergenic 45.0%
ATCCCACAAGGTGTATACGT+AGG - chr2.4:4133103-4133122 MS.gene85201:CDS 45.0%
ATCCCTACGTATACACCTTG+TGG + chr2.4:4133109-4133128 None:intergenic 45.0%
CCATATCCATCTGCATATGG+AGG + chr2.4:4133369-4133388 None:intergenic 45.0%
CCTCCATATGCAGATGGATA+TGG - chr2.4:4133366-4133385 MS.gene85201:CDS 45.0%
CTAGCATGGATGGAAGACAT+TGG - chr2.4:4132576-4132595 MS.gene85201:intron 45.0%
CTGAACATGAGATGCATTCG+AGG - chr2.4:4132088-4132107 MS.gene85201:intron 45.0%
GAAGGCAAACATGGAGTTCA+TGG - chr2.4:4132481-4132500 MS.gene85201:intron 45.0%
GCAGAGAAAGGTCCTCTTTA+TGG - chr2.4:4133490-4133509 MS.gene85201:CDS 45.0%
TATCCATCTGCATATGGAGG+TGG + chr2.4:4133366-4133385 None:intergenic 45.0%
TCCCACAAGGTGTATACGTA+GGG - chr2.4:4133104-4133123 MS.gene85201:CDS 45.0%
TCCCTACGTATACACCTTGT+GGG + chr2.4:4133108-4133127 None:intergenic 45.0%
TCCTCTAACAATGCTGGATG+AGG + chr2.4:4131922-4131941 None:intergenic 45.0%
TCTAACAATGCTGGATGAGG+TGG + chr2.4:4131919-4131938 None:intergenic 45.0%
! TAGAGAAAGCGTGGGAGTTA+AGG + chr2.4:4132958-4132977 None:intergenic 45.0%
! TTGGTGGCTCTCTATGAACA+CGG + chr2.4:4131822-4131841 None:intergenic 45.0%
!! AATTTTGGTAGTCCAGAGGG+GGG - chr2.4:4133336-4133355 MS.gene85201:CDS 45.0%
!! CTTTTGACTGGCAGTGTTGA+GGG - chr2.4:4133073-4133092 MS.gene85201:CDS 45.0%
!! TCTTTTGACTGGCAGTGTTG+AGG - chr2.4:4133072-4133091 MS.gene85201:CDS 45.0%
CACCAATGAGTTCCCGAAGA+GGG - chr2.4:4131836-4131855 MS.gene85201:CDS 50.0%
CATGGAACAGAGGCAGTCAA+TGG - chr2.4:4132499-4132518 MS.gene85201:intron 50.0%
CTTCCGATGGATACCCTCTT+CGG + chr2.4:4131852-4131871 None:intergenic 50.0%
GAAAGGTCCTCTTTATGGGG+TGG - chr2.4:4133495-4133514 MS.gene85201:CDS 50.0%
GGAATATGCTGTCGTGGCAT+TGG + chr2.4:4131898-4131917 None:intergenic 50.0%
GTTTAGGCTGCTGCAGAGAA+AGG - chr2.4:4133478-4133497 MS.gene85201:CDS 50.0%
TACCCTCTTCGGGAACTCAT+TGG + chr2.4:4131841-4131860 None:intergenic 50.0%
TGAGGTGGAATATGCTGTCG+TGG + chr2.4:4131904-4131923 None:intergenic 50.0%
TGCAGCATGCTGAAATGAGG+AGG - chr2.4:4131953-4131972 MS.gene85201:intron 50.0%
TTCCCACCTCCATATGCAGA+TGG - chr2.4:4133360-4133379 MS.gene85201:CDS 50.0%
TTCCGATGGATACCCTCTTC+GGG + chr2.4:4131851-4131870 None:intergenic 50.0%
TTTCTCAACTTCTCGGGCCA+TGG + chr2.4:4132540-4132559 None:intergenic 50.0%
! ATATGCTGTCGTGGCATTGG+TGG + chr2.4:4131895-4131914 None:intergenic 50.0%
!! TTCCCGAAGAGGGTATCCAT+CGG - chr2.4:4131846-4131865 MS.gene85201:CDS 50.0%
ACCAAGAACTGATGCGCGCT+AGG - chr2.4:4132325-4132344 MS.gene85201:intron 55.0%
ACCTAGCGCGCATCAGTTCT+TGG + chr2.4:4132329-4132348 None:intergenic 55.0%
ACTGATGCGCGCTAGGTTTG+TGG - chr2.4:4132332-4132351 MS.gene85201:intron 55.0%
CCACCAATGAGTTCCCGAAG+AGG - chr2.4:4131835-4131854 MS.gene85201:CDS 55.0%
CCTCTTCGGGAACTCATTGG+TGG + chr2.4:4131838-4131857 None:intergenic 55.0%
CGAGCATAAGGACCTTCCGA+TGG + chr2.4:4131865-4131884 None:intergenic 55.0%
GATGGAAGACATTGGGGAGC+TGG - chr2.4:4132584-4132603 MS.gene85201:intron 55.0%
GCAGTACCCACCCCATAAAG+AGG + chr2.4:4133505-4133524 None:intergenic 55.0%
GCATGCTGAAATGAGGAGGC+TGG - chr2.4:4131957-4131976 MS.gene85201:intron 55.0%
TCGGAAGGTCCTTATGCTCG+CGG - chr2.4:4131865-4131884 MS.gene85201:CDS 55.0%
TGAAATGAGGAGGCTGGTGG+CGG - chr2.4:4131963-4131982 MS.gene85201:intron 55.0%
TGCTGAAATGAGGAGGCTGG+TGG - chr2.4:4131960-4131979 MS.gene85201:intron 55.0%
! ATGGGGTGGGTACTGCTTCT+AGG - chr2.4:4133509-4133528 MS.gene85201:CDS 55.0%
! TTGACTGGCAGTGTTGAGGG+TGG - chr2.4:4133076-4133095 MS.gene85201:CDS 55.0%
!! CGAAGAGGGTATCCATCGGA+AGG - chr2.4:4131850-4131869 MS.gene85201:CDS 55.0%
!! GCTGAGCTTGCTAGCATGGA+TGG - chr2.4:4132566-4132585 MS.gene85201:intron 55.0%
!! TCGTGCTGAGCTTGCTAGCA+TGG - chr2.4:4132562-4132581 MS.gene85201:intron 55.0%
AGACCTTGCAGCTGCGAAGG+AGG - chr2.4:4132026-4132045 MS.gene85201:CDS 60.0%
CGGAAGGTCCTTATGCTCGC+GGG - chr2.4:4131866-4131885 MS.gene85201:CDS 60.0%
! ACTGGCAGTGTTGAGGGTGG+TGG - chr2.4:4133079-4133098 MS.gene85201:CDS 60.0%
! GAGGGTGGTGGTATCCCACA+AGG - chr2.4:4133091-4133110 MS.gene85201:CDS 60.0%
!! TGCTGTCGTGGCATTGGTGG+TGG + chr2.4:4131892-4131911 None:intergenic 60.0%
GCGAGACCTTGCAGCTGCGA+AGG - chr2.4:4132023-4132042 MS.gene85201:CDS 65.0%
GCTCCTCCTTCGCAGCTGCA+AGG + chr2.4:4132032-4132051 None:intergenic 65.0%
!! GGAGGCTGGTGGCGGATAAC+AGG - chr2.4:4131971-4131990 MS.gene85201:intron 65.0%
!! GGCTGGTGGCGGATAACAGG+AGG - chr2.4:4131974-4131993 MS.gene85201:CDS 65.0%
GGAGGTGGGAAACCCCCCTC+TGG + chr2.4:4133351-4133370 None:intergenic 70.0%
! GGTGGTGGCCCGCGAGCATA+AGG + chr2.4:4131877-4131896 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 4131805 4133567 4131805 ID=MS.gene85201
chr2.4 mRNA 4131805 4133567 4131805 ID=MS.gene85201.t1;Parent=MS.gene85201
chr2.4 exon 4133026 4133567 4133026 ID=MS.gene85201.t1.exon1;Parent=MS.gene85201.t1
chr2.4 CDS 4133026 4133567 4133026 ID=cds.MS.gene85201.t1;Parent=MS.gene85201.t1
chr2.4 exon 4132767 4132912 4132767 ID=MS.gene85201.t1.exon2;Parent=MS.gene85201.t1
chr2.4 CDS 4132767 4132912 4132767 ID=cds.MS.gene85201.t1;Parent=MS.gene85201.t1
chr2.4 exon 4131974 4132053 4131974 ID=MS.gene85201.t1.exon3;Parent=MS.gene85201.t1
chr2.4 CDS 4131974 4132053 4131974 ID=cds.MS.gene85201.t1;Parent=MS.gene85201.t1
chr2.4 exon 4131805 4131888 4131805 ID=MS.gene85201.t1.exon4;Parent=MS.gene85201.t1
chr2.4 CDS 4131805 4131888 4131805 ID=cds.MS.gene85201.t1;Parent=MS.gene85201.t1
Gene Sequence

>MS.gene85201

ATGGGATCAAGACACCGTGTTCATAGAGAGCCACCAATGAGTTCCCGAAGAGGGTATCCATCGGAAGGTCCTTATGCTCGCGGGCCACCACCAATGCCACGACAGCATATTCCACCTCATCCAGCATTGTTAGAGGAAGAGCTTGAAGTGCAGCATGCTGAAATGAGGAGGCTGGTGGCGGATAACAGGAGGTTGATTGATGATCGGATGACGTTGCAGCGAGACCTTGCAGCTGCGAAGGAGGAGCTTCATCGTATGAATTTAGCGATTGGCGATATTCGTGCTGAACATGAGATGCATTCGAGGGAGCTTGTTGATAAAAATATGAAGTTGGAGTCTGATCTTAGGTCGACTGAGCCGTTGAAGAATGAAGTTATACAACTGCGAGCTGAAGTTCAAAAACTTAGTAGTATCAAACAAGAACTTTCTGGGAAGGTCCAGACACTTACGAAAGATGTTTCTAGGTTACAGTCTGATAATCAACAAATTCCTTCAATGAGATCTGAGATTGATGGTTTGCACCAAGAACTGATGCGCGCTAGGTTTGTGGATTGATTGCTAACACGGCTTTTGTTTTCTATCTTTAAGCATCCTTTTGGTTGGGATTTCTGCATTTGTTTTCTTGAACTGATAATGTTGCATTTTTGTTTTATAGAACTATGGTGGATTATGAAAAGAAGGCAAACATGGAGTTCATGGAACAGAGGCAGTCAATGGAGAAAAACATGGTTTCCATGGCCCGAGAAGTTGAGAAACTTCGTGCTGAGCTTGCTAGCATGGATGGAAGACATTGGGGAGCTGGTATGACATGTTTTTTATATTGTCCTTTGCAGAATTTATCATCCTTCAGTCGTTAACTCCATATGTATTTACTAGCATAGCAATACAATCTGTGACTTGTCAAAACAATTGTAATAGTCTGATCTAATGAAATGACATGAATAATAGGTGAGTCATTTAATAAAAGACATGAAAAACGGTAAAAATGACATTTCTTCTATTATTTTAAAGACCCTTCATAATTGTCTGAAAGATGGTTGGGGGTGTCAGTAACCGTAATCACATAATACACAAAAGTGGCAGTTTTTTAGAATTTAAGGCAATTGGTAACTGAATTTAAAATAATATAATTTTTTCCTATTCTTAATCACCTTAACTCCCACGCTTTCTCTAACTTGTCTATGTGATGATTTTGAGAACGGCAGAATTTTAGTATTCATCGAGGGAGTTCATTAGTGATAACAGGGCTTGTACTCATATCTTTCGATCTTTTGACTGGCAGTGTTGAGGGTGGTGGTATCCCACAAGGTGTATACGTAGGGATATGCCAAAAATGCAGATACTGTAGCATCCTATTATTTGTGTTAATTGGATACACATATAATATAGACACATCATGATGTTTGGAGTATCTGTGCTTCATTGACTGAACACTGAAATTATTAATTATATAGTTACTGAGATATTATTCTTGTTCTTTTACCCCTTTTTTAACGTTTGCTTCATTCATGCAGGTTTGCCTTATGGAACAAATTTTGGTAGTCCAGAGGGGGGTTTCCCACCTCCATATGCAGATGGATATGGTGTTCACTTGGTAAGTGTCTTCCTTAACACAATAATTTTAGACCTCGTATTCAATTTTCCAAGGTTTCATTTACTTTGCTTGTGCTTCTGTTTAGGCTGCTGCAGAGAAAGGTCCTCTTTATGGGGTGGGTACTGCTTCTAGGAAAGCACACGAGAAGCCTCGCTCAAACCGTCGTTGA
Protein sequence

>MS.gene85201.t1

MGSRHRVHREPPMSSRRGYPSEGPYARGPPPMPRQHIPPHPALLEEELEVQHAEMRRLVADNRRLIDDRMTLQRDLAAAKEELHRMNLAIGDIRAEHEMHSRELVDKNMKLESDLRSTEPLKNEVIQLRAEVQKLSSIKQELSGKVQTLTKDVSRLQSDNQQIPSMRSEIDGLHQELMRARTMVDYEKKANMEFMEQRQSMEKNMVSMAREVEKLRAELASMDGRHWGAGLPYGTNFGSPEGGFPPPYADGYGVHLAAAEKGPLYGVGTASRKAHEKPRSNRR