AlfalfaGEDB | MS.gene85266

M. sativa cultivar XinJiangDaYe / MS.gene85266

KEGG: -
GO: -
PFAM: PF03514.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85266.t1	XP_013465454.1	96.6	444	15	0	1	444	9	452	4.50E-251	876.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85266.t1	Q9SZF7	51.1	405	166	9	59	444	139	530	3.5e-111	403.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85266.t1	A0A072VBU1	96.6	444	15	0	1	444	9	452	3.3e-251	876.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene85266.t1	TF	GRAS

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049350	MS.gene85266	0.836529	8.87E-57	-1.69E-46
MS.gene049711	MS.gene85266	0.816036	6.80E-52	-1.69E-46
MS.gene050901	MS.gene85266	0.802673	5.02E-49	-1.69E-46
MS.gene051719	MS.gene85266	0.813622	2.33E-51	-1.69E-46
MS.gene051720	MS.gene85266	0.80212	6.52E-49	-1.69E-46
MS.gene052000	MS.gene85266	0.957679	1.51E-115	-1.69E-46
MS.gene053144	MS.gene85266	0.803398	3.55E-49	-1.69E-46
MS.gene053536	MS.gene85266	0.805357	1.39E-49	-1.69E-46
MS.gene053538	MS.gene85266	0.803258	3.80E-49	-1.69E-46
MS.gene055465	MS.gene85266	0.816403	5.63E-52	-1.69E-46
MS.gene055537	MS.gene85266	0.811051	8.49E-51	-1.69E-46
MS.gene05894	MS.gene85266	0.827264	1.72E-54	-1.69E-46
MS.gene060519	MS.gene85266	0.804953	1.69E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85266.t1	MTR_2g099110	97.297	444	12	0	1	444	9	452	0.0	906
MS.gene85266.t1	MTR_5g015490	54.774	398	153	7	60	444	108	491	1.19e-148	432
MS.gene85266.t1	MTR_4g097080	55.499	391	151	8	62	444	129	504	5.53e-147	428
MS.gene85266.t1	MTR_2g089100	28.169	426	250	9	54	444	40	444	1.12e-51	181
MS.gene85266.t1	MTR_1g029420	29.306	389	245	8	65	443	224	592	9.07e-51	181
MS.gene85266.t1	MTR_4g095500	28.953	487	269	14	13	444	5	469	1.39e-47	170
MS.gene85266.t1	MTR_2g082090	29.381	388	244	8	65	442	211	578	4.88e-46	168
MS.gene85266.t1	MTR_3g089055	28.021	389	250	8	65	443	202	570	1.23e-45	167
MS.gene85266.t1	MTR_3g089055	28.021	389	250	8	65	443	197	565	1.23e-45	166
MS.gene85266.t1	MTR_5g094450	28.608	395	244	10	65	443	160	532	1.46e-42	157
MS.gene85266.t1	MTR_5g097480	28.462	390	245	9	66	443	177	544	4.06e-42	156
MS.gene85266.t1	MTR_5g015950	27.021	433	244	11	65	444	34	447	1.07e-41	154
MS.gene85266.t1	MTR_3g053270	27.021	433	244	11	65	444	34	447	1.07e-41	154
MS.gene85266.t1	MTR_3g056110	26.478	389	256	8	65	443	174	542	4.22e-39	148
MS.gene85266.t1	MTR_4g133660	28.316	392	244	11	65	442	174	542	1.84e-38	146
MS.gene85266.t1	MTR_8g020840	27.317	410	250	13	58	443	163	548	7.18e-38	145
MS.gene85266.t1	MTR_7g074650	27.676	383	240	10	69	444	441	793	2.39e-37	145
MS.gene85266.t1	MTR_8g442410	28.462	390	246	10	67	443	156	525	3.62e-37	142
MS.gene85266.t1	MTR_7g027190	25.570	395	256	9	66	443	300	673	5.70e-34	135
MS.gene85266.t1	MTR_2g026250	26.804	388	253	10	67	443	229	596	6.11e-34	134
MS.gene85266.t1	MTR_3g065980	27.342	395	242	9	67	443	173	540	2.49e-33	132
MS.gene85266.t1	MTR_4g077760	28.753	393	237	11	65	443	192	555	4.59e-33	131
MS.gene85266.t1	MTR_4g102790	24.422	389	267	8	66	444	292	663	9.22e-33	131
MS.gene85266.t1	MTR_2g097310	27.481	393	246	11	66	444	272	639	8.12e-32	128
MS.gene85266.t1	MTR_4g064200	27.179	390	251	11	66	444	284	651	1.43e-31	127
MS.gene85266.t1	MTR_2g097350	28.827	392	242	13	66	444	274	641	3.93e-31	126
MS.gene85266.t1	MTR_3g072710	27.320	388	239	14	76	442	134	499	4.18e-31	125
MS.gene85266.t1	MTR_1g069725	26.316	437	276	16	14	442	64	462	5.21e-30	121
MS.gene85266.t1	MTR_4g076020	26.786	392	223	17	69	444	87	430	5.48e-30	121
MS.gene85266.t1	MTR_7g062120	25.943	424	278	11	36	444	339	741	1.59e-29	122
MS.gene85266.t1	MTR_4g104020	25.616	406	254	12	59	443	142	520	3.57e-28	117
MS.gene85266.t1	MTR_4g064120	25.195	385	257	10	42	409	226	596	4.06e-28	117
MS.gene85266.t1	MTR_2g097463	27.247	356	225	10	66	408	290	624	1.31e-27	116
MS.gene85266.t1	MTR_5g058860	28.438	429	258	18	39	442	92	496	1.77e-27	115
MS.gene85266.t1	MTR_2g097467	25.281	356	232	8	66	408	289	623	1.87e-27	115
MS.gene85266.t1	MTR_4g064160	24.422	389	265	8	66	444	316	685	2.91e-27	115
MS.gene85266.t1	MTR_2g097410	25.062	403	267	10	56	444	361	742	3.01e-27	115
MS.gene85266.t1	MTR_4g064180	25.549	364	235	11	42	387	226	571	1.09e-26	113
MS.gene85266.t1	MTR_4g064150	25.320	391	263	12	66	444	361	734	1.19e-26	113
MS.gene85266.t1	MTR_2g097390	26.410	390	254	10	66	444	275	642	2.14e-26	112
MS.gene85266.t1	MTR_2g097473	24.760	416	271	12	2	407	239	622	9.02e-26	110
MS.gene85266.t1	MTR_2g034250	24.557	395	260	12	67	443	211	585	3.67e-23	102
MS.gene85266.t1	MTR_2g034260	23.291	395	264	13	67	443	211	584	7.08e-23	101
MS.gene85266.t1	MTR_1g096030	25.683	366	236	12	97	443	111	459	1.61e-22	100
MS.gene85266.t1	MTR_2g034280	26.298	289	196	8	67	343	204	487	7.04e-22	98.6
MS.gene85266.t1	MTR_4g076140	31.481	216	136	5	57	270	39	244	1.36e-21	97.4
MS.gene85266.t1	MTR_5g009080	26.754	228	157	4	57	283	47	265	5.76e-21	95.5
MS.gene85266.t1	MTR_3g022830	22.299	435	256	10	67	443	26	436	5.60e-20	92.0
MS.gene85266.t1	MTR_2g097380	25.625	320	199	8	73	384	231	519	1.12e-19	92.0
MS.gene85266.t1	MTR_7g057230	25.837	418	256	13	45	442	273	656	1.47e-18	88.6
MS.gene85266.t1	MTR_0092s0100	23.316	386	257	9	67	443	376	731	1.16e-17	86.3
MS.gene85266.t1	MTR_0092s0100	23.316	386	257	9	67	443	373	728	1.46e-17	85.9
MS.gene85266.t1	MTR_6g047750	24.378	402	252	13	63	443	292	662	2.00e-16	82.0
MS.gene85266.t1	MTR_8g093070	25.634	355	218	15	102	444	177	497	8.45e-16	79.7
MS.gene85266.t1	MTR_4g026485	21.763	363	254	8	85	443	288	624	5.07e-15	77.8
MS.gene85266.t1	MTR_1g086970	21.644	365	260	10	88	443	131	478	7.93e-15	76.6
MS.gene85266.t1	MTR_8g077940	23.828	256	176	8	85	331	201	446	6.69e-12	67.8
MS.gene85266.t1	MTR_7g109580	22.432	370	237	12	93	443	216	554	1.12e-11	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85266.t1	AT4G37650	51.111	405	166	9	59	444	139	530	1.73e-141	415
MS.gene85266.t1	AT3G49950	30.976	410	236	12	59	444	23	409	9.95e-57	193
MS.gene85266.t1	AT5G48150	28.900	391	241	9	67	443	123	490	4.07e-47	169
MS.gene85266.t1	AT5G48150	28.900	391	241	9	67	443	123	490	4.07e-47	169
MS.gene85266.t1	AT5G48150	28.900	391	241	9	67	443	123	490	4.07e-47	169
MS.gene85266.t1	AT5G48150	28.900	391	241	9	67	443	123	490	4.07e-47	169
MS.gene85266.t1	AT2G04890	28.756	386	242	9	66	443	53	413	4.13e-47	167
MS.gene85266.t1	AT1G50600	29.381	388	244	9	66	443	159	526	1.23e-44	163
MS.gene85266.t1	AT1G50600	29.381	388	244	9	66	443	230	597	3.21e-44	163
MS.gene85266.t1	AT1G50600	29.381	388	244	9	66	443	234	601	3.68e-44	163
MS.gene85266.t1	AT1G21450	27.962	422	266	12	37	443	195	593	1.24e-42	159
MS.gene85266.t1	AT3G13840	29.953	424	244	13	40	443	120	510	2.52e-42	156
MS.gene85266.t1	AT3G54220	29.925	401	237	15	56	444	283	651	2.43e-38	147
MS.gene85266.t1	AT3G03450	27.225	382	253	9	67	443	184	545	1.21e-37	144
MS.gene85266.t1	AT4G17230	27.551	392	250	10	65	444	157	526	7.56e-37	142
MS.gene85266.t1	AT4G17230	27.551	392	250	10	65	444	157	526	7.56e-37	142
MS.gene85266.t1	AT5G17490	27.559	381	247	9	67	443	161	516	2.31e-36	140
MS.gene85266.t1	AT1G14920	27.506	389	238	10	67	443	173	529	1.06e-34	135
MS.gene85266.t1	AT3G46600	28.718	390	246	10	66	444	4	372	3.32e-34	132
MS.gene85266.t1	AT1G66350	27.487	382	241	11	67	443	156	506	4.71e-34	134
MS.gene85266.t1	AT3G46600	28.718	390	246	10	66	444	212	580	2.15e-33	132
MS.gene85266.t1	AT3G46600	28.718	390	246	10	66	444	180	548	3.04e-33	132
MS.gene85266.t1	AT2G01570	26.753	385	246	9	67	443	225	581	4.83e-33	131
MS.gene85266.t1	AT5G59450	28.061	392	250	10	66	444	227	599	1.14e-32	130
MS.gene85266.t1	AT5G41920	27.990	418	252	16	46	443	12	400	4.39e-31	124
MS.gene85266.t1	AT1G07530	24.485	437	293	12	23	444	352	766	4.08e-29	120
MS.gene85266.t1	AT1G63100	24.619	394	263	9	66	442	277	653	5.07e-29	120
MS.gene85266.t1	AT1G63100	24.619	394	263	9	66	442	252	628	5.81e-29	120
MS.gene85266.t1	AT2G29065	25.127	394	264	10	66	444	252	629	1.94e-28	118
MS.gene85266.t1	AT5G66770	27.273	418	261	14	43	443	193	584	3.13e-28	117
MS.gene85266.t1	AT4G08250	27.907	387	232	14	75	442	118	476	6.65e-28	115
MS.gene85266.t1	AT2G29060	26.343	391	258	9	66	444	284	656	9.27e-28	116
MS.gene85266.t1	AT2G29060	26.343	391	258	9	66	444	321	693	9.85e-28	116
MS.gene85266.t1	AT2G37650	25.831	391	255	12	66	444	335	702	1.52e-26	113
MS.gene85266.t1	AT2G37650	25.831	391	255	12	66	444	347	714	1.59e-26	113
MS.gene85266.t1	AT1G07520	26.276	392	261	10	66	444	319	695	1.60e-26	113
MS.gene85266.t1	AT1G07520	26.276	392	261	10	66	444	318	694	1.64e-26	113
MS.gene85266.t1	AT1G55580	27.301	326	208	9	132	443	135	445	1.69e-26	111
MS.gene85266.t1	AT1G07520	26.276	392	261	10	66	444	154	530	2.08e-26	112
MS.gene85266.t1	AT1G07520	26.276	392	261	10	66	444	154	530	2.08e-26	112
MS.gene85266.t1	AT3G50650	26.944	360	227	11	97	443	206	542	2.46e-24	105
MS.gene85266.t1	AT1G50420	29.268	246	163	4	30	270	16	255	1.18e-23	103
MS.gene85266.t1	AT5G52510	23.775	408	252	11	63	443	265	640	3.48e-20	94.0
MS.gene85266.t1	AT4G00150	27.451	255	151	9	85	329	226	456	1.04e-12	70.5
MS.gene85266.t1	AT3G60630	24.920	313	197	12	146	443	333	622	3.55e-12	68.9

Find 65 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGGAGATGATCATATTTC+AGG	0.247824	2.4:+4989273	MS.gene85266:CDS
AGTGTTCAAGAAGCATAATT+AGG	0.262613	2.4:+4989052	MS.gene85266:CDS
AAGAAGCATAATTAGGGTTT+TGG	0.264830	2.4:+4989059	MS.gene85266:CDS
TGCACACTCTTTGAGAAGTT+TGG	0.280317	2.4:-4988209	None:intergenic
AACGAGTTATCTTCACCTTA+TGG	0.280967	2.4:+4988286	MS.gene85266:CDS
GGAGAAATTTGCTAGGCTTA+TGG	0.293692	2.4:+4988699	MS.gene85266:CDS
GAGAAATTTGCTAGGCTTAT+GGG	0.296804	2.4:+4988700	MS.gene85266:CDS
GTAAGTCCATGGACAACTTT+TGG	0.310503	2.4:+4988463	MS.gene85266:CDS
GAAGGAGGAGCCAGTTGTTT+GGG	0.360276	2.4:+4989311	MS.gene85266:CDS
GGAAGGAGGAGCCAGTTGTT+TGG	0.371169	2.4:+4989310	MS.gene85266:CDS
GCCTACTCTTCTAGAAGCTT+TGG	0.372153	2.4:+4988579	MS.gene85266:CDS
ATTAACCTCTCATTACTTGT+TGG	0.376305	2.4:-4989018	None:intergenic
CATGTTGATGCCCAAACAAC+TGG	0.384320	2.4:-4989321	None:intergenic
GTGTTCAAGAAGCATAATTA+GGG	0.390341	2.4:+4989053	MS.gene85266:CDS
ATCGTGATCAAGATGGTAAA+TGG	0.422295	2.4:+4988182	MS.gene85266:CDS
TGACAACAATTGTGAGCTTA+AGG	0.423250	2.4:-4988627	None:intergenic
GTATTCTTTGAAGTATTGTA+AGG	0.423643	2.4:-4988031	None:intergenic
TTCTAGTGTTGTTGTTATGA+AGG	0.438949	2.4:+4988660	MS.gene85266:CDS
CTAATAACGTTCAACTCAAA+AGG	0.439883	2.4:-4988727	None:intergenic
TGATGTATGCATCATGTCTA+TGG	0.450632	2.4:-4988113	None:intergenic
GCAATAGCTTTGAATTGTGT+TGG	0.455338	2.4:+4988808	MS.gene85266:CDS
ACGTCAAAGCGTTGCTAAAG+AGG	0.462927	2.4:+4989220	MS.gene85266:CDS
GCAACACACTTTGTACTCAA+TGG	0.478846	2.4:+4988557	MS.gene85266:CDS
GTTGTTGTTATGAAGGAAGT+TGG	0.482632	2.4:+4988667	MS.gene85266:CDS
AACAATGGTTTGAAAGGCTT+AGG	0.484650	2.4:+4989154	MS.gene85266:CDS
TTTGGACACGTAGCATCAAA+TGG	0.490444	2.4:+4988481	MS.gene85266:CDS
ACACTCTTTGAGAAGTTTGG+AGG	0.491229	2.4:-4988206	None:intergenic
GAAGGAAGTTGGTCAAAGAA+TGG	0.494144	2.4:+4988678	MS.gene85266:CDS
TCAACAAATGTCACAACTCT+AGG	0.495147	2.4:-4988895	None:intergenic
GAGGAAAACAATGGTTTGAA+AGG	0.495452	2.4:+4989148	MS.gene85266:CDS
ATGAGAGGTTAATGTTGGAA+AGG	0.501619	2.4:+4989028	MS.gene85266:CDS
GTGACATTTGTTGAGGAAGA+AGG	0.504902	2.4:+4988904	MS.gene85266:CDS
TGGATTTAGTGATGATGTTG+TGG	0.508345	2.4:+4989194	MS.gene85266:CDS
GTGACCAGATTGTGTTGCCT+TGG	0.516301	2.4:-4988356	None:intergenic
TATTGTGATAAAAGAGAGAG+AGG	0.525178	2.4:+4989129	MS.gene85266:CDS
TGAGAGGTTAATGTTGGAAA+GGG	0.526258	2.4:+4989029	MS.gene85266:CDS
GAATTAACGAAAGAGAGATT+AGG	0.532150	2.4:+4988769	MS.gene85266:CDS
TGGTCACTTGTTGTGCCTCA+AGG	0.536151	2.4:+4989255	MS.gene85266:CDS
CGTTTAACATCCAAAGAAGA+TGG	0.546279	2.4:-4988267	None:intergenic
GAGAGATTAGGAATTCAAGA+AGG	0.556850	2.4:+4988781	MS.gene85266:CDS
AAGTAATGAGAGGTTAATGT+TGG	0.563161	2.4:+4989023	MS.gene85266:CDS
GTTAATACTCAAGTTTCAAG+AGG	0.563644	2.4:+4988441	MS.gene85266:CDS
AAAGAATGGAGAAATTTGCT+AGG	0.565724	2.4:+4988692	MS.gene85266:CDS
CAGTTGTTTGGGCATCAACA+TGG	0.572778	2.4:+4989322	MS.gene85266:CDS
TAGAGTTGTGACATTTGTTG+AGG	0.579320	2.4:+4988897	MS.gene85266:CDS
ACGTGTCCAAAAGTTGTCCA+TGG	0.583351	2.4:-4988469	None:intergenic
TTCTTGCTGAATCAAAAGAG+TGG	0.585703	2.4:-4988417	None:intergenic
TTCAACCAACAAGTAATGAG+AGG	0.588837	2.4:+4989013	MS.gene85266:CDS
TTTGATGATGATGAAGATGA+TGG	0.595058	2.4:+4989096	MS.gene85266:CDS
AAAGAGAGAGAGGAAAACAA+TGG	0.597298	2.4:+4989139	MS.gene85266:CDS
TTTCAGGAATGTACCTCACA+TGG	0.601917	2.4:+4989289	MS.gene85266:CDS
ACATGTCATCGTGATCAAGA+TGG	0.610985	2.4:+4988175	MS.gene85266:CDS
TTAACATCCAAAGAAGATGG+TGG	0.611767	2.4:-4988264	None:intergenic
AGTTTCAAGAGGTAAGTCCA+TGG	0.613365	2.4:+4988452	MS.gene85266:CDS
GCCAAAGCTTCTAGAAGAGT+AGG	0.613903	2.4:-4988580	None:intergenic
TAAAGAGGTATCAAGCTGGT+TGG	0.616512	2.4:+4989235	MS.gene85266:CDS
AGGAATGTACCTCACATGGA+AGG	0.622436	2.4:+4989293	MS.gene85266:CDS
CTGGCTCCTCCTTCCATGTG+AGG	0.635263	2.4:-4989302	None:intergenic
TTGCTAAAGAGGTATCAAGC+TGG	0.647082	2.4:+4989231	MS.gene85266:CDS
AATGTACCTCACATGGAAGG+AGG	0.650550	2.4:+4989296	MS.gene85266:CDS
GAAATATGATCATCTCCTTG+AGG	0.654963	2.4:-4989270	None:intergenic
GATGATGAAGATGATGGTGG+TGG	0.659497	2.4:+4989102	MS.gene85266:CDS
GATGATGATGAAGATGATGG+TGG	0.660969	2.4:+4989099	MS.gene85266:CDS
ACAATTGTGAGCTTAAGGTG+AGG	0.710121	2.4:-4988622	None:intergenic
ACATCATCACTAAATCCACA+AGG	0.718156	2.4:-4989189	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTATTTTGAGATGTTAG+AGG	+	chr2.4:4988984-4989003	MS.gene85266:CDS	20.0%
!!!	GAAAATAGCTATTATTTTGA+TGG	-	chr2.4:4988078-4988097	None:intergenic	20.0%
!!	TTTTGATCAATATCTCCATA+AGG	-	chr2.4:4988304-4988323	None:intergenic	25.0%
!!!	GTATTCTTTGAAGTATTGTA+AGG	-	chr2.4:4988034-4988053	None:intergenic	25.0%
	AAGTAATGAGAGGTTAATGT+TGG	+	chr2.4:4989023-4989042	MS.gene85266:CDS	30.0%
	ATTAACCTCTCATTACTTGT+TGG	-	chr2.4:4989021-4989040	None:intergenic	30.0%
	CTAATAACGTTCAACTCAAA+AGG	-	chr2.4:4988730-4988749	None:intergenic	30.0%
	GAATTAACGAAAGAGAGATT+AGG	+	chr2.4:4988769-4988788	MS.gene85266:CDS	30.0%
	GTTAATACTCAAGTTTCAAG+AGG	+	chr2.4:4988441-4988460	MS.gene85266:CDS	30.0%
	TATTGTGATAAAAGAGAGAG+AGG	+	chr2.4:4989129-4989148	MS.gene85266:CDS	30.0%
!	AAAGAATGGAGAAATTTGCT+AGG	+	chr2.4:4988692-4988711	MS.gene85266:CDS	30.0%
!	AGTGTTCAAGAAGCATAATT+AGG	+	chr2.4:4989052-4989071	MS.gene85266:CDS	30.0%
!	GTGTTCAAGAAGCATAATTA+GGG	+	chr2.4:4989053-4989072	MS.gene85266:CDS	30.0%
!	TTCTAGTGTTGTTGTTATGA+AGG	+	chr2.4:4988660-4988679	MS.gene85266:CDS	30.0%
!!	AAGAAGCATAATTAGGGTTT+TGG	+	chr2.4:4989059-4989078	MS.gene85266:CDS	30.0%
!!	TTTGATGATGATGAAGATGA+TGG	+	chr2.4:4989096-4989115	MS.gene85266:CDS	30.0%
	AAAGAGAGAGAGGAAAACAA+TGG	+	chr2.4:4989139-4989158	MS.gene85266:CDS	35.0%
	AACAATGGTTTGAAAGGCTT+AGG	+	chr2.4:4989154-4989173	MS.gene85266:CDS	35.0%
	AACGAGTTATCTTCACCTTA+TGG	+	chr2.4:4988286-4988305	MS.gene85266:CDS	35.0%
	ACATCATCACTAAATCCACA+AGG	-	chr2.4:4989192-4989211	None:intergenic	35.0%
	ATCGTGATCAAGATGGTAAA+TGG	+	chr2.4:4988182-4988201	MS.gene85266:CDS	35.0%
	ATGAGAGGTTAATGTTGGAA+AGG	+	chr2.4:4989028-4989047	MS.gene85266:CDS	35.0%
	CAAAAATCCACCATCTTCTT+TGG	+	chr2.4:4988257-4988276	MS.gene85266:CDS	35.0%
	CAAGGAGATGATCATATTTC+AGG	+	chr2.4:4989273-4989292	MS.gene85266:CDS	35.0%
	CGTTTAACATCCAAAGAAGA+TGG	-	chr2.4:4988270-4988289	None:intergenic	35.0%
	GAAATATGATCATCTCCTTG+AGG	-	chr2.4:4989273-4989292	None:intergenic	35.0%
	GAGAGATTAGGAATTCAAGA+AGG	+	chr2.4:4988781-4988800	MS.gene85266:CDS	35.0%
	GAGGAAAACAATGGTTTGAA+AGG	+	chr2.4:4989148-4989167	MS.gene85266:CDS	35.0%
	TCAACAAATGTCACAACTCT+AGG	-	chr2.4:4988898-4988917	None:intergenic	35.0%
	TGACAACAATTGTGAGCTTA+AGG	-	chr2.4:4988630-4988649	None:intergenic	35.0%
	TGAGAGGTTAATGTTGGAAA+GGG	+	chr2.4:4989029-4989048	MS.gene85266:CDS	35.0%
	TGATGTATGCATCATGTCTA+TGG	-	chr2.4:4988116-4988135	None:intergenic	35.0%
	TTAACATCCAAAGAAGATGG+TGG	-	chr2.4:4988267-4988286	None:intergenic	35.0%
	TTCAACCAACAAGTAATGAG+AGG	+	chr2.4:4989013-4989032	MS.gene85266:CDS	35.0%
	TTCTTGCTGAATCAAAAGAG+TGG	-	chr2.4:4988420-4988439	None:intergenic	35.0%
!	AGGAATGAATTTTCACCTTG+TGG	+	chr2.4:4989174-4989193	MS.gene85266:CDS	35.0%
!	GTTGTTGTTATGAAGGAAGT+TGG	+	chr2.4:4988667-4988686	MS.gene85266:CDS	35.0%
!	TAGAGTTGTGACATTTGTTG+AGG	+	chr2.4:4988897-4988916	MS.gene85266:CDS	35.0%
!	TCTACAAGCTCTATTTTCCA+AGG	+	chr2.4:4988339-4988358	MS.gene85266:CDS	35.0%
!	TGGATTTAGTGATGATGTTG+TGG	+	chr2.4:4989194-4989213	MS.gene85266:CDS	35.0%
!!	GAGAAATTTGCTAGGCTTAT+GGG	+	chr2.4:4988700-4988719	MS.gene85266:CDS	35.0%
!!	GCAATAGCTTTGAATTGTGT+TGG	+	chr2.4:4988808-4988827	MS.gene85266:CDS	35.0%
!!!	GCTATTTTAGAAGCTTTGGA+AGG	+	chr2.4:4988505-4988524	MS.gene85266:CDS	35.0%
!!!	TGGTGCTATTTTAGAAGCTT+TGG	+	chr2.4:4988501-4988520	MS.gene85266:CDS	35.0%
	ACAATTGTGAGCTTAAGGTG+AGG	-	chr2.4:4988625-4988644	None:intergenic	40.0%
	ACATGTCATCGTGATCAAGA+TGG	+	chr2.4:4988175-4988194	MS.gene85266:CDS	40.0%
	AGTTTCAAGAGGTAAGTCCA+TGG	+	chr2.4:4988452-4988471	MS.gene85266:CDS	40.0%
	GCAACACACTTTGTACTCAA+TGG	+	chr2.4:4988557-4988576	MS.gene85266:CDS	40.0%
	TTTCAGGAATGTACCTCACA+TGG	+	chr2.4:4989289-4989308	MS.gene85266:CDS	40.0%
	TTTGGACACGTAGCATCAAA+TGG	+	chr2.4:4988481-4988500	MS.gene85266:CDS	40.0%
!	ACACTCTTTGAGAAGTTTGG+AGG	-	chr2.4:4988209-4988228	None:intergenic	40.0%
!	GAAGGAAGTTGGTCAAAGAA+TGG	+	chr2.4:4988678-4988697	MS.gene85266:CDS	40.0%
!	GATGATGATGAAGATGATGG+TGG	+	chr2.4:4989099-4989118	MS.gene85266:CDS	40.0%
!	GTAAGTCCATGGACAACTTT+TGG	+	chr2.4:4988463-4988482	MS.gene85266:CDS	40.0%
!	GTGACATTTGTTGAGGAAGA+AGG	+	chr2.4:4988904-4988923	MS.gene85266:CDS	40.0%
!	TGCACACTCTTTGAGAAGTT+TGG	-	chr2.4:4988212-4988231	None:intergenic	40.0%
!	TTTTCCAAGGCAACACAATC+TGG	+	chr2.4:4988352-4988371	MS.gene85266:CDS	40.0%
!!	GGAGAAATTTGCTAGGCTTA+TGG	+	chr2.4:4988699-4988718	MS.gene85266:CDS	40.0%
!!	TAAAGAGGTATCAAGCTGGT+TGG	+	chr2.4:4989235-4989254	MS.gene85266:CDS	40.0%
!!	TTGCTAAAGAGGTATCAAGC+TGG	+	chr2.4:4989231-4989250	MS.gene85266:CDS	40.0%
	AATGTACCTCACATGGAAGG+AGG	+	chr2.4:4989296-4989315	MS.gene85266:CDS	45.0%
	ACGTGTCCAAAAGTTGTCCA+TGG	-	chr2.4:4988472-4988491	None:intergenic	45.0%
	AGGAATGTACCTCACATGGA+AGG	+	chr2.4:4989293-4989312	MS.gene85266:CDS	45.0%
	CAGTTGTTTGGGCATCAACA+TGG	+	chr2.4:4989322-4989341	MS.gene85266:CDS	45.0%
	CATGTTGATGCCCAAACAAC+TGG	-	chr2.4:4989324-4989343	None:intergenic	45.0%
!	GCCAAAGCTTCTAGAAGAGT+AGG	-	chr2.4:4988583-4988602	None:intergenic	45.0%
!	GCCTACTCTTCTAGAAGCTT+TGG	+	chr2.4:4988579-4988598	MS.gene85266:CDS	45.0%
!!	ACGTCAAAGCGTTGCTAAAG+AGG	+	chr2.4:4989220-4989239	MS.gene85266:CDS	45.0%
!!	GATGATGAAGATGATGGTGG+TGG	+	chr2.4:4989102-4989121	MS.gene85266:CDS	45.0%
	GAAGGAGGAGCCAGTTGTTT+GGG	+	chr2.4:4989311-4989330	MS.gene85266:CDS	50.0%
	GTGACCAGATTGTGTTGCCT+TGG	-	chr2.4:4988359-4988378	None:intergenic	50.0%
!!	TGGTCACTTGTTGTGCCTCA+AGG	+	chr2.4:4989255-4989274	MS.gene85266:CDS	50.0%
	GGAAGGAGGAGCCAGTTGTT+TGG	+	chr2.4:4989310-4989329	MS.gene85266:CDS	55.0%
	CTGGCTCCTCCTTCCATGTG+AGG	-	chr2.4:4989305-4989324	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	4988019	4989353	4988019	ID=MS.gene85266
chr2.4	mRNA	4988019	4989353	4988019	ID=MS.gene85266.t1;Parent=MS.gene85266
chr2.4	exon	4988019	4989353	4988019	ID=MS.gene85266.t1.exon1;Parent=MS.gene85266.t1
chr2.4	CDS	4988019	4989353	4988019	ID=cds.MS.gene85266.t1;Parent=MS.gene85266.t1

Gene Sequence

>MS.gene85266

ATGGAGATAAGTCCTTACAATACTTCAAAGAATACAACGCACTTGAAGAAGTTGAACCATCAAAATAATAGCTATTTTCTTCATGAAGCTTCAACCATAGACATGATGCATACATCATCAAGCAATCATCAAACATCTTCAGATAATTCTAGTGAGACATGTCATCGTGATCAAGATGGTAAATGGTCCTCCAAACTTCTCAAAGAGTGTGCAATTGCAATATCTAATAGAGACTCATCAAAAATCCACCATCTTCTTTGGATGTTAAACGAGTTATCTTCACCTTATGGAGATATTGATCAAAAACTAGCTTCATATTTTCTACAAGCTCTATTTTCCAAGGCAACACAATCTGGTCACAAGTGTTACAAAACACTATCTTCAATCGCTTACAAAAACCACTCTTTTGATTCAGCAAGAAAGTTAATACTCAAGTTTCAAGAGGTAAGTCCATGGACAACTTTTGGACACGTAGCATCAAATGGTGCTATTTTAGAAGCTTTGGAAGGAGAGAAAAAACTTCATATAATTGATATTAGCAACACACTTTGTACTCAATGGCCTACTCTTCTAGAAGCTTTGGCAACAAGAAATGATGAAACTCCTCACCTTAAGCTCACAATTGTTGTCACAAATAATAGTTCTAGTGTTGTTGTTATGAAGGAAGTTGGTCAAAGAATGGAGAAATTTGCTAGGCTTATGGGAGTACCTTTTGAGTTGAACGTTATTAGTGATTTGAAACATATACGAGAATTAACGAAAGAGAGATTAGGAATTCAAGAAGGTGAAGCAATAGCTTTGAATTGTGTTGGAGCATTGAGAAAAGTTGAAGTTGAAGAAAGAGAAAGTGTGATTCAATTTTTCAAATCACTTAGTCCTAGAGTTGTGACATTTGTTGAGGAAGAAGGTGATTTTTGTAGTGATGACTTTGTTAAATGTTTTGAAGAGTGTCTTAAGTTTTATAGAATTTATTTTGAGATGTTAGAGGAGAGTTTTCAACCAACAAGTAATGAGAGGTTAATGTTGGAAAGGGAGTGTTCAAGAAGCATAATTAGGGTTTTGGCTTGTGATCATGAATTTGATGATGATGAAGATGATGGTGGTGGTGATTATTGTGATAAAAGAGAGAGAGGAAAACAATGGTTTGAAAGGCTTAGGAATGAATTTTCACCTTGTGGATTTAGTGATGATGTTGTGGATGACGTCAAAGCGTTGCTAAAGAGGTATCAAGCTGGTTGGTCACTTGTTGTGCCTCAAGGAGATGATCATATTTCAGGAATGTACCTCACATGGAAGGAGGAGCCAGTTGTTTGGGCATCAACATGGAAACCCTAG

Protein sequence

>MS.gene85266.t1

MEISPYNTSKNTTHLKKLNHQNNSYFLHEASTIDMMHTSSSNHQTSSDNSSETCHRDQDGKWSSKLLKECAIAISNRDSSKIHHLLWMLNELSSPYGDIDQKLASYFLQALFSKATQSGHKCYKTLSSIAYKNHSFDSARKLILKFQEVSPWTTFGHVASNGAILEALEGEKKLHIIDISNTLCTQWPTLLEALATRNDETPHLKLTIVVTNNSSSVVVMKEVGQRMEKFARLMGVPFELNVISDLKHIRELTKERLGIQEGEAIALNCVGALRKVEVEERESVIQFFKSLSPRVVTFVEEEGDFCSDDFVKCFEECLKFYRIYFEMLEESFQPTSNERLMLERECSRSIIRVLACDHEFDDDEDDGGGDYCDKRERGKQWFERLRNEFSPCGFSDDVVDDVKALLKRYQAGWSLVVPQGDDHISGMYLTWKEEPVVWASTWKP