Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85692.t1 | XP_028762943.1 | 46 | 348 | 72 | 3 | 1 | 232 | 1 | 348 | 1.10E-70 | 276.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85692.t1 | A0A1U8PVE2 | 50.2 | 269 | 83 | 2 | 1 | 218 | 1 | 269 | 6.4e-65 | 256.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene85692 | 0.804416 | 2.18E-49 | -1.69E-46 |
| MS.gene050221 | MS.gene85692 | 0.814235 | 1.71E-51 | -1.69E-46 |
| MS.gene050561 | MS.gene85692 | 0.80241 | 5.69E-49 | -1.69E-46 |
| MS.gene050657 | MS.gene85692 | 0.824981 | 6.01E-54 | -1.69E-46 |
| MS.gene051744 | MS.gene85692 | 0.808437 | 3.10E-50 | -1.69E-46 |
| MS.gene052037 | MS.gene85692 | 0.802815 | 4.69E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene85692 | 0.81851 | 1.89E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene85692 | 0.806513 | 7.93E-50 | -1.69E-46 |
| MS.gene059272 | MS.gene85692 | 0.842156 | 3.07E-58 | -1.69E-46 |
| MS.gene059570 | MS.gene85692 | 0.805554 | 1.26E-49 | -1.69E-46 |
| MS.gene059774 | MS.gene85692 | 0.807813 | 4.21E-50 | -1.69E-46 |
| MS.gene060167 | MS.gene85692 | 0.82141 | 4.10E-53 | -1.69E-46 |
| MS.gene06052 | MS.gene85692 | 0.80259 | 5.22E-49 | -1.69E-46 |
| MS.gene060697 | MS.gene85692 | 0.806901 | 6.57E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85692.t1 | MTR_7g062720 | 59.740 | 154 | 56 | 1 | 1 | 154 | 1 | 148 | 2.01e-62 | 192 |
| MS.gene85692.t1 | MTR_5g061150 | 66.372 | 113 | 38 | 0 | 20 | 132 | 10 | 122 | 3.15e-47 | 153 |
| MS.gene85692.t1 | MTR_1g036690 | 57.317 | 82 | 28 | 3 | 152 | 232 | 5 | 80 | 9.61e-23 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGGCTCTTAATATTTC+TGG | 0.080746 | 6.1:-17487504 | MS.gene85692:CDS |
| TTCGGCATTTAAATGAATTT+TGG | 0.166319 | 6.1:+17487452 | None:intergenic |
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 6.1:-17486609 | MS.gene85692:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 6.1:-17486617 | MS.gene85692:CDS |
| ATGGTTATATTTGCGTTTAC+AGG | 0.259438 | 6.1:-17487229 | MS.gene85692:CDS |
| AAATTGTCCCAAAGATCTTT+TGG | 0.267031 | 6.1:+17487306 | None:intergenic |
| TATTTCTGGAAATAATTATC+TGG | 0.282933 | 6.1:-17487490 | MS.gene85692:CDS |
| TAGAAGATCCAAAAGATCTT+TGG | 0.283634 | 6.1:-17487314 | MS.gene85692:CDS |
| TATAGGTGGGGTTCTATTCC+TGG | 0.331534 | 6.1:+17486455 | None:intergenic |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 6.1:+17486640 | None:intergenic |
| CTGGAAATAATTATCTGGCT+TGG | 0.347827 | 6.1:-17487485 | MS.gene85692:CDS |
| TGGAAATAATTATCTGGCTT+GGG | 0.353242 | 6.1:-17487484 | MS.gene85692:CDS |
| AACCATTCATTACGAGCATT+AGG | 0.386252 | 6.1:+17487246 | None:intergenic |
| GAAAATGATGAAGATGATTA+TGG | 0.391347 | 6.1:-17486565 | MS.gene85692:CDS |
| TTTGGGACAATTTAAAAGAA+AGG | 0.395342 | 6.1:-17487296 | MS.gene85692:CDS |
| AGAAGATCCAAAAGATCTTT+GGG | 0.400056 | 6.1:-17487313 | MS.gene85692:CDS |
| TGCTATCGTTGTGGAGGAAA+AGG | 0.414829 | 6.1:-17486688 | MS.gene85692:CDS |
| CCTTCGTCACCACCTTCATA+AGG | 0.433246 | 6.1:-17487361 | MS.gene85692:CDS |
| ATTAACACTGTGTGGAGAAA+AGG | 0.440407 | 6.1:-17487154 | MS.gene85692:intron |
| CAAAACAACTGAACAACAAA+AGG | 0.454927 | 6.1:-17487400 | MS.gene85692:CDS |
| AATTGTATCTCCATGCCCTT+CGG | 0.460742 | 6.1:+17487434 | None:intergenic |
| AGAAATCAGCAGCATCTAAG+TGG | 0.464577 | 6.1:+17486527 | None:intergenic |
| AATGTTGTAAAGAATGATAT+TGG | 0.471241 | 6.1:-17486739 | MS.gene85692:CDS |
| TGAAGATGATTATGGTAATA+CGG | 0.476728 | 6.1:-17486557 | MS.gene85692:CDS |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 6.1:-17486608 | MS.gene85692:CDS |
| CATTTAAATGCCGAAGGGCA+TGG | 0.494088 | 6.1:-17487444 | MS.gene85692:CDS |
| GTTGTGGAGGAAAAGGTCAT+TGG | 0.504176 | 6.1:-17486681 | MS.gene85692:CDS |
| TTCCTAATGCTCGTAATGAA+TGG | 0.506622 | 6.1:-17487248 | MS.gene85692:CDS |
| CATTAGAGATGAAAATATCC+AGG | 0.532865 | 6.1:-17486473 | MS.gene85692:CDS |
| CATGGAGATACAATTAAAGA+AGG | 0.533844 | 6.1:-17487426 | MS.gene85692:CDS |
| AAAATTCATTTAAATGCCGA+AGG | 0.537172 | 6.1:-17487450 | MS.gene85692:CDS |
| GTCTGTAATCGAATATAACT+CGG | 0.574961 | 6.1:-17487199 | MS.gene85692:CDS |
| AACTGAACAACAAAAGGCTA+AGG | 0.582581 | 6.1:-17487394 | MS.gene85692:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 6.1:-17486697 | MS.gene85692:CDS |
| ATTCATTACGAGCATTAGGA+AGG | 0.589984 | 6.1:+17487250 | None:intergenic |
| CCTTATGAAGGTGGTGACGA+AGG | 0.598722 | 6.1:+17487361 | None:intergenic |
| AGTATTTGCTATCGTTGTGG+AGG | 0.599695 | 6.1:-17486694 | MS.gene85692:CDS |
| GAAATCAGCAGCATCTAAGT+GGG | 0.621669 | 6.1:+17486528 | None:intergenic |
| AAATTCATTTAAATGCCGAA+GGG | 0.631745 | 6.1:-17487449 | MS.gene85692:CDS |
| GTGGTGACGAAGGAATATCA+TGG | 0.663314 | 6.1:+17487371 | None:intergenic |
| ACTTCTCTATTAACACTGTG+TGG | 0.668278 | 6.1:-17487162 | MS.gene85692:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGTAAAGAATGATAT+TGG | - | chr6.1:17487257-17487276 | MS.gene85692:CDS | 20.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | - | chr6.1:17487388-17487407 | MS.gene85692:CDS | 20.0% |
| !!! | TATTTCTGGAAATAATTATC+TGG | - | chr6.1:17486506-17486525 | MS.gene85692:CDS | 20.0% |
| ! | AAAATTCATTTAAATGCCGA+AGG | - | chr6.1:17486546-17486565 | MS.gene85692:CDS | 25.0% |
| ! | AAATTCATTTAAATGCCGAA+GGG | - | chr6.1:17486547-17486566 | MS.gene85692:CDS | 25.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | - | chr6.1:17487387-17487406 | MS.gene85692:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | - | chr6.1:17487431-17487450 | MS.gene85692:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+CGG | - | chr6.1:17487439-17487458 | MS.gene85692:CDS | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | + | chr6.1:17487359-17487378 | None:intergenic | 25.0% |
| !! | TATTTTCATCTCTAATGAGA+TGG | + | chr6.1:17487520-17487539 | None:intergenic | 25.0% |
| !! | TTACAACATTTTTCATCTTC+TGG | + | chr6.1:17487247-17487266 | None:intergenic | 25.0% |
| !! | TTCGGCATTTAAATGAATTT+TGG | + | chr6.1:17486547-17486566 | None:intergenic | 25.0% |
| !! | TTTGGGACAATTTAAAAGAA+AGG | - | chr6.1:17486700-17486719 | MS.gene85692:CDS | 25.0% |
| !!! | CATTTTTTAGATCCTTATGA+AGG | + | chr6.1:17486650-17486669 | None:intergenic | 25.0% |
| !!! | GAGAAAATGTGTTTTTGTTT+TGG | + | chr6.1:17487223-17487242 | None:intergenic | 25.0% |
| AGAAGATCCAAAAGATCTTT+GGG | - | chr6.1:17486683-17486702 | MS.gene85692:CDS | 30.0% | |
| ATGGTTATATTTGCGTTTAC+AGG | - | chr6.1:17486767-17486786 | MS.gene85692:intron | 30.0% | |
| CAAAACAACTGAACAACAAA+AGG | - | chr6.1:17486596-17486615 | MS.gene85692:CDS | 30.0% | |
| CATGGAGATACAATTAAAGA+AGG | - | chr6.1:17486570-17486589 | MS.gene85692:CDS | 30.0% | |
| CATTAGAGATGAAAATATCC+AGG | - | chr6.1:17487523-17487542 | MS.gene85692:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | - | chr6.1:17487379-17487398 | MS.gene85692:CDS | 30.0% | |
| GTCTGTAATCGAATATAACT+CGG | - | chr6.1:17486797-17486816 | MS.gene85692:intron | 30.0% | |
| TACATTAGATGCATGAAAAG+TGG | + | chr6.1:17486901-17486920 | None:intergenic | 30.0% | |
| TAGAAGATCCAAAAGATCTT+TGG | - | chr6.1:17486682-17486701 | MS.gene85692:CDS | 30.0% | |
| ! | AAATTGTCCCAAAGATCTTT+TGG | + | chr6.1:17486693-17486712 | None:intergenic | 30.0% |
| ! | AAGATCTTTTGGATCTTCTA+TGG | + | chr6.1:17486682-17486701 | None:intergenic | 30.0% |
| ! | GCTTTTGATGAAAAATCACT+CGG | - | chr6.1:17487007-17487026 | MS.gene85692:intron | 30.0% |
| ! | TGGAAATAATTATCTGGCTT+GGG | - | chr6.1:17486512-17486531 | MS.gene85692:CDS | 30.0% |
| ! | TTTGAGGCTCTTAATATTTC+TGG | - | chr6.1:17486492-17486511 | MS.gene85692:CDS | 30.0% |
| ! | TTTTTAGATCCTTATGAAGG+TGG | + | chr6.1:17486647-17486666 | None:intergenic | 30.0% |
| !! | GGTTTTAATCGTAATCACAA+TGG | - | chr6.1:17487173-17487192 | MS.gene85692:CDS | 30.0% |
| !!! | ACCTTTTAAAACCCTTTTCA+CGG | + | chr6.1:17486941-17486960 | None:intergenic | 30.0% |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | + | chr6.1:17487382-17487401 | None:intergenic | 30.0% |
| AACCATTCATTACGAGCATT+AGG | + | chr6.1:17486753-17486772 | None:intergenic | 35.0% | |
| AACTGAACAACAAAAGGCTA+AGG | - | chr6.1:17486602-17486621 | MS.gene85692:CDS | 35.0% | |
| ACTTCTCTATTAACACTGTG+TGG | - | chr6.1:17486834-17486853 | MS.gene85692:intron | 35.0% | |
| ATTAACACTGTGTGGAGAAA+AGG | - | chr6.1:17486842-17486861 | MS.gene85692:intron | 35.0% | |
| ATTCATTACGAGCATTAGGA+AGG | + | chr6.1:17486749-17486768 | None:intergenic | 35.0% | |
| CTGGAAATAATTATCTGGCT+TGG | - | chr6.1:17486511-17486530 | MS.gene85692:CDS | 35.0% | |
| TTCCTAATGCTCGTAATGAA+TGG | - | chr6.1:17486748-17486767 | MS.gene85692:CDS | 35.0% | |
| ! | AGATGGTCAATCTTTTCATC+AGG | + | chr6.1:17487503-17487522 | None:intergenic | 35.0% |
| ! | CCTCAAAATCAAGTTTTGCA+AGG | + | chr6.1:17486479-17486498 | None:intergenic | 35.0% |
| ! | CCTTGCAAAACTTGATTTTG+AGG | - | chr6.1:17486476-17486495 | MS.gene85692:CDS | 35.0% |
| ! | CTTTTGGATCTTCTATGGTA+AGG | + | chr6.1:17486677-17486696 | None:intergenic | 35.0% |
| !!! | ACCGTGAAAAGGGTTTTAAA+AGG | - | chr6.1:17486937-17486956 | MS.gene85692:intron | 35.0% |
| AATTGTATCTCCATGCCCTT+CGG | + | chr6.1:17486565-17486584 | None:intergenic | 40.0% | |
| AGAAATCAGCAGCATCTAAG+TGG | + | chr6.1:17487472-17487491 | None:intergenic | 40.0% | |
| CAGCAACAATACCGTGAAAA+GGG | - | chr6.1:17486927-17486946 | MS.gene85692:intron | 40.0% | |
| CTTAATATCATGCCTGCTTG+TGG | - | chr6.1:17486968-17486987 | MS.gene85692:intron | 40.0% | |
| GAAATCAGCAGCATCTAAGT+GGG | + | chr6.1:17487471-17487490 | None:intergenic | 40.0% | |
| GATCACTATGGACAAAATCG+TGG | - | chr6.1:17487080-17487099 | MS.gene85692:intron | 40.0% | |
| GTAGCAACACATGATCACTA+TGG | - | chr6.1:17487068-17487087 | MS.gene85692:intron | 40.0% | |
| ! | GAAGGATGACAGTTTTCTGA+TGG | + | chr6.1:17486731-17486750 | None:intergenic | 40.0% |
| ! | GTGTTGCTACATTCACTTCT+GGG | + | chr6.1:17487058-17487077 | None:intergenic | 40.0% |
| ! | TATTTTGTTCTGCCACAAGC+AGG | + | chr6.1:17486983-17487002 | None:intergenic | 40.0% |
| ! | TGTGTTGCTACATTCACTTC+TGG | + | chr6.1:17487059-17487078 | None:intergenic | 40.0% |
| !! | AGTATTTGCTATCGTTGTGG+AGG | - | chr6.1:17487302-17487321 | MS.gene85692:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | - | chr6.1:17487299-17487318 | MS.gene85692:CDS | 40.0% |
| CATTTAAATGCCGAAGGGCA+TGG | - | chr6.1:17486552-17486571 | MS.gene85692:CDS | 45.0% | |
| GCAGCAACAATACCGTGAAA+AGG | - | chr6.1:17486926-17486945 | MS.gene85692:intron | 45.0% | |
| GTTGTGGAGGAAAAGGTCAT+TGG | - | chr6.1:17487315-17487334 | MS.gene85692:CDS | 45.0% | |
| TATGGACAAAATCGTGGTCG+TGG | - | chr6.1:17487086-17487105 | MS.gene85692:intron | 45.0% | |
| TGCTATCGTTGTGGAGGAAA+AGG | - | chr6.1:17487308-17487327 | MS.gene85692:CDS | 45.0% | |
| TTTCACGGTATTGTTGCTGC+AGG | + | chr6.1:17486926-17486945 | None:intergenic | 45.0% | |
| ! | GCTACATTCACTTCTGGGAA+TGG | + | chr6.1:17487053-17487072 | None:intergenic | 45.0% |
| ! | GTGGTGACGAAGGAATATCA+TGG | + | chr6.1:17486628-17486647 | None:intergenic | 45.0% |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | + | chr6.1:17487348-17487367 | None:intergenic | 45.0% |
| ACGTGGTCGTGGTTATGGAA+GGG | - | chr6.1:17487139-17487158 | MS.gene85692:intron | 50.0% | |
| CCTTCGTCACCACCTTCATA+AGG | - | chr6.1:17486635-17486654 | MS.gene85692:CDS | 50.0% | |
| ! | CCTTATGAAGGTGGTGACGA+AGG | + | chr6.1:17486638-17486657 | None:intergenic | 50.0% |
| ! | TATGGAAGGGGACGTGGTTT+TGG | - | chr6.1:17487152-17487171 | MS.gene85692:intron | 50.0% |
| CGTGGTCGTGGTTATGGAAG+GGG | - | chr6.1:17487140-17487159 | MS.gene85692:intron | 55.0% | |
| GACGTGGTCGTGGTTATGGA+AGG | - | chr6.1:17487138-17487157 | MS.gene85692:intron | 55.0% | |
| ! | GGGAATGGAGCACTACCAGT+GGG | + | chr6.1:17487038-17487057 | None:intergenic | 55.0% |
| ! | TGGGAATGGAGCACTACCAG+TGG | + | chr6.1:17487039-17487058 | None:intergenic | 55.0% |
| CAAAATCGTGGTCGTGGCCG+TGG | - | chr6.1:17487092-17487111 | MS.gene85692:intron | 60.0% | |
| CACGTCCACGACCATGACCA+CGG | + | chr6.1:17487112-17487131 | None:intergenic | 60.0% | |
| CGTGGACGTGGTCGTGGTTA+TGG | - | chr6.1:17487134-17487153 | MS.gene85692:intron | 60.0% | |
| CGTGGTTATGGAAGGGGACG+TGG | - | chr6.1:17487146-17487165 | MS.gene85692:intron | 60.0% | |
| ! | AATCACTCGGCTCGTCCCAC+TGG | - | chr6.1:17487020-17487039 | MS.gene85692:intron | 60.0% |
| CATGGTCGTGGACGTGGACG+TGG | - | chr6.1:17487116-17487135 | MS.gene85692:intron | 65.0% | |
| CGTGGTCATGGTCGTGGACG+TGG | - | chr6.1:17487110-17487129 | MS.gene85692:intron | 65.0% | |
| CGTGGACGTGGACGTGGACG+TGG | - | chr6.1:17487122-17487141 | MS.gene85692:intron | 70.0% | |
| CGTGGACGTGGACGTGGTCG+TGG | - | chr6.1:17487128-17487147 | MS.gene85692:intron | 70.0% | |
| CGTGGCCGTGGTCATGGTCG+TGG | - | chr6.1:17487104-17487123 | MS.gene85692:intron | 70.0% | |
| CGTGGTCGTGGCCGTGGTCA+TGG | - | chr6.1:17487098-17487117 | MS.gene85692:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 17486468 | 17487550 | 17486468 | ID=MS.gene85692 |
| chr6.1 | mRNA | 17486468 | 17487550 | 17486468 | ID=MS.gene85692.t1;Parent=MS.gene85692 |
| chr6.1 | exon | 17487155 | 17487550 | 17487155 | ID=MS.gene85692.t1.exon1;Parent=MS.gene85692.t1 |
| chr6.1 | CDS | 17487155 | 17487550 | 17487155 | ID=cds.MS.gene85692.t1;Parent=MS.gene85692.t1 |
| chr6.1 | exon | 17486468 | 17486770 | 17486468 | ID=MS.gene85692.t1.exon2;Parent=MS.gene85692.t1 |
| chr6.1 | CDS | 17486468 | 17486770 | 17486468 | ID=cds.MS.gene85692.t1;Parent=MS.gene85692.t1 |
| Gene Sequence |
| Protein sequence |
