Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86172.t1 | RHN50189.1 | 97.3 | 147 | 4 | 0 | 1 | 147 | 310 | 456 | 1.40E-76 | 295.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86172.t1 | Q9ZWR8 | 37.9 | 145 | 82 | 2 | 3 | 144 | 325 | 464 | 7.8e-23 | 108.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86172.t1 | A0A072U6Q7 | 97.3 | 147 | 4 | 0 | 1 | 147 | 310 | 456 | 1.0e-76 | 295.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049281 | MS.gene86172 | 0.804911 | 1.72E-49 | -1.69E-46 |
| MS.gene051364 | MS.gene86172 | 0.813591 | 2.37E-51 | -1.69E-46 |
| MS.gene051366 | MS.gene86172 | 0.805702 | 1.17E-49 | -1.69E-46 |
| MS.gene051367 | MS.gene86172 | 0.815481 | 9.04E-52 | -1.69E-46 |
| MS.gene060595 | MS.gene86172 | 0.80142 | 9.08E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAATTAATGCAAATTATTTT+GGG | - | 42568:10506-10525 | MS.gene86172:CDS | 15.0% |
| !!! | TATAAGATTTTTTCTATTAC+CGG | - | 42568:10709-10728 | MS.gene86172:CDS | 15.0% |
| !! | AATTGATGTGTTAAATGATT+TGG | - | 42568:10612-10631 | MS.gene86172:CDS | 20.0% |
| !!! | AAATAATTTGCATTAATTGG+TGG | + | 42568:10505-10524 | MS.gene86172:intergenic | 20.0% |
| !!! | AGGTGTATAATTTTGATTTT+GGG | - | 42568:10746-10765 | MS.gene86172:CDS | 20.0% |
| !!! | ATAAGATTTTTTCTATTACC+GGG | - | 42568:10710-10729 | MS.gene86172:CDS | 20.0% |
| !!! | CCAAAATAATTTGCATTAAT+TGG | + | 42568:10508-10527 | MS.gene86172:intergenic | 20.0% |
| !!! | CCAATTAATGCAAATTATTT+TGG | - | 42568:10505-10524 | MS.gene86172:CDS | 20.0% |
| !! | CTTTTTATCTTTCAGGAAAT+AGG | - | 42568:10818-10837 | MS.gene86172:CDS | 25.0% |
| !! | GTGTTAAATGATTTGGAAAA+TGG | - | 42568:10619-10638 | MS.gene86172:CDS | 25.0% |
| !! | TGTTAAATGGTTTGGAAAAT+TGG | - | 42568:10644-10663 | MS.gene86172:CDS | 25.0% |
| !!! | GAGGTGTATAATTTTGATTT+TGG | - | 42568:10745-10764 | MS.gene86172:CDS | 25.0% |
| !!! | GTATAATTTTGATTTTGGGT+GGG | - | 42568:10750-10769 | MS.gene86172:CDS | 25.0% |
| !!! | TAAGAAAGTTGATGTTACTT+CGG | - | 42568:10780-10799 | MS.gene86172:CDS | 25.0% |
| !!! | TATAATTTTGATTTTGGGTG+GGG | - | 42568:10751-10770 | MS.gene86172:CDS | 25.0% |
| !!! | TGTATAATTTTGATTTTGGG+TGG | - | 42568:10749-10768 | MS.gene86172:CDS | 25.0% |
| AAATGGTGTGTTAAATGGTT+TGG | - | 42568:10636-10655 | MS.gene86172:CDS | 30.0% | |
| AAATTATACACCTCAAACCT+AGG | + | 42568:10739-10758 | MS.gene86172:intergenic | 30.0% | |
| GAAGTAACATCAACTTTCTT+AGG | + | 42568:10781-10800 | MS.gene86172:intergenic | 30.0% | |
| GGTTTATTAGTGCTCTTAAA+GGG | - | 42568:10587-10606 | MS.gene86172:CDS | 30.0% | |
| TTGGAAAATGGTGTGTTAAA+TGG | - | 42568:10631-10650 | MS.gene86172:CDS | 30.0% | |
| ! | GCAATCTAAAACATCTAGAA+CGG | - | 42568:10678-10697 | MS.gene86172:CDS | 30.0% |
| ATTAGTTGAGCATGAAACTG+AGG | - | 42568:10546-10565 | MS.gene86172:CDS | 35.0% | |
| GACTTTGAACAAGCAACAAA+TGG | - | 42568:10870-10889 | MS.gene86172:CDS | 35.0% | |
| GGGTTTATTAGTGCTCTTAA+AGG | - | 42568:10586-10605 | MS.gene86172:CDS | 35.0% | |
| TCAGGAAATAGGAATAACGA+TGG | - | 42568:10829-10848 | MS.gene86172:CDS | 35.0% | |
| TTTGAACAAGCAACAAATGG+AGG | - | 42568:10873-10892 | MS.gene86172:CDS | 35.0% | |
| ! | TTTGCTCGACTTTTTGTTCA+AGG | - | 42568:10898-10917 | MS.gene86172:CDS | 35.0% |
| CCTGAAAGATAAAAAGCTCC+TGG | + | 42568:10814-10833 | MS.gene86172:intergenic | 40.0% | |
| GGAAATAGGAATAACGATGG+TGG | - | 42568:10832-10851 | MS.gene86172:CDS | 40.0% | |
| ! | AGGCATTGTTAGAAGATGGT+GGG | - | 42568:10566-10585 | MS.gene86172:CDS | 40.0% |
| ! | CCAGGAGCTTTTTATCTTTC+AGG | - | 42568:10811-10830 | MS.gene86172:CDS | 40.0% |
| !! | AACGATGGTGGAATTGAGAT+TGG | - | 42568:10844-10863 | MS.gene86172:CDS | 40.0% |
| !! | ACTGAGGCATTGTTAGAAGA+TGG | - | 42568:10562-10581 | MS.gene86172:CDS | 40.0% |
| TTTCTATTACCGGGTCACCT+AGG | - | 42568:10719-10738 | MS.gene86172:CDS | 45.0% | |
| !! | GAGGCATTGTTAGAAGATGG+TGG | - | 42568:10565-10584 | MS.gene86172:CDS | 45.0% |
| !! | GTTACTTCGGTTGATAGACC+AGG | - | 42568:10793-10812 | MS.gene86172:CDS | 45.0% |
| CACCTCAAACCTAGGTGACC+CGG | + | 42568:10731-10750 | MS.gene86172:intergenic | 55.0% | |
| TACCGGGTCACCTAGGTTTG+AGG | - | 42568:10726-10745 | MS.gene86172:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42568 | gene | 10489 | 10932 | 10489 | ID=MS.gene86172 |
| 42568 | mRNA | 10489 | 10932 | 10489 | ID=MS.gene86172.t1;Parent=MS.gene86172 |
| 42568 | exon | 10489 | 10932 | 10489 | ID=MS.gene86172.t1.exon1;Parent=MS.gene86172.t1 |
| 42568 | CDS | 10489 | 10932 | 10489 | ID=cds.MS.gene86172.t1;Parent=MS.gene86172.t1 |
| Gene Sequence |
| Protein sequence |
