Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86945.t1 | XP_003597930.2 | 99.4 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 3.40E-93 | 350.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86945.t1 | P42322 | 37.8 | 172 | 100 | 2 | 1 | 167 | 1 | 170 | 1.6e-27 | 124.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86945.t1 | B7FH75 | 99.4 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 2.5e-93 | 350.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05548 | MS.gene86945 | 0.810152 | 1.33E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene83474 | MS.gene86945 | PPI |
| MS.gene82121 | MS.gene86945 | PPI |
| MS.gene37035 | MS.gene86945 | PPI |
| MS.gene028111 | MS.gene86945 | PPI |
| MS.gene020092 | MS.gene86945 | PPI |
| MS.gene86945 | MS.gene72809 | PPI |
| MS.gene022826 | MS.gene86945 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86945.t1 | MTR_2g104130 | 99.429 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 5.87e-128 | 356 |
| MS.gene86945.t1 | MTR_4g130410 | 70.286 | 175 | 52 | 0 | 1 | 175 | 1 | 175 | 1.37e-91 | 264 |
| MS.gene86945.t1 | MTR_4g130410 | 71.429 | 161 | 46 | 0 | 1 | 161 | 1 | 161 | 5.36e-84 | 245 |
| MS.gene86945.t1 | MTR_1g076650 | 27.517 | 149 | 94 | 5 | 22 | 161 | 3 | 146 | 1.56e-11 | 59.7 |
| MS.gene86945.t1 | MTR_1g071150 | 27.517 | 149 | 95 | 5 | 21 | 161 | 2 | 145 | 2.35e-11 | 58.9 |
| MS.gene86945.t1 | MTR_1g041285 | 26.797 | 153 | 98 | 5 | 22 | 165 | 3 | 150 | 2.44e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86945.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene86945.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene86945.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene86945.t1 | AT3G22930 | 27.152 | 151 | 96 | 5 | 24 | 165 | 28 | 173 | 5.16e-12 | 61.6 |
Find 24 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACAGGTTCTTGGCCATTC+TGG | 0.182957 | 2.3:-773561 | MS.gene86945:intron |
| TGTACTAAATGCAGATAAAA+AGG | 0.239607 | 2.3:+774569 | None:intergenic |
| GACATTGGATGATTTCATTA+AGG | 0.296420 | 2.3:-773757 | MS.gene86945:intron |
| TTAAATTTGTCACAGGTTCT+TGG | 0.342309 | 2.3:-773570 | MS.gene86945:intron |
| TTTGATTTCTCAGGAAGTCT+TGG | 0.366861 | 2.3:-773823 | MS.gene86945:intron |
| TTGATTTCTCAGGAAGTCTT+GGG | 0.378929 | 2.3:-773822 | MS.gene86945:intron |
| AGGTTACTCAAGATGGTTGA+TGG | 0.393812 | 2.3:-774615 | MS.gene86945:CDS |
| TGTTATGAATCCACTTTCTC+AGG | 0.398536 | 2.3:-774984 | MS.gene86945:intron |
| GGTGTCTTTCAAAGACATAT+TGG | 0.407546 | 2.3:-774234 | MS.gene86945:CDS |
| ATTTGATTCAGTTTCTCAAC+AGG | 0.411656 | 2.3:-775098 | MS.gene86945:intron |
| GAAGTTCTAAAAGATTTGTC+TGG | 0.444669 | 2.3:-774212 | MS.gene86945:CDS |
| ATCCTGCTTCTTTCAGAACC+TGG | 0.474604 | 2.3:+773799 | None:intergenic |
| TTCTCAGGAAGTCTTGGGCC+AGG | 0.475351 | 2.3:-773817 | MS.gene86945:intron |
| CAACTTGACAGAAATGCTAA+AGG | 0.478282 | 2.3:-775046 | MS.gene86945:CDS |
| TTTGCAGAGGTTACTCAAGA+TGG | 0.478552 | 2.3:-774622 | MS.gene86945:intron |
| TGATATGATACCTGAGAAAG+TGG | 0.501757 | 2.3:+774974 | None:intergenic |
| TCTGCATTTAGTACAAAAGC+TGG | 0.505330 | 2.3:-774561 | MS.gene86945:CDS |
| GCTAACTCAATATGATATTG+AGG | 0.520808 | 2.3:-775229 | MS.gene86945:CDS |
| GGCCAGGTTCTGAAAGAAGC+AGG | 0.530644 | 2.3:-773801 | MS.gene86945:CDS |
| GTATATGATTCAGATCGTAA+TGG | 0.556045 | 2.3:-774260 | MS.gene86945:CDS |
| AAAAGACTGCTACTTGACAT+TGG | 0.563411 | 2.3:-773772 | MS.gene86945:CDS |
| AGTGGATTCATAACAAACTC+AGG | 0.575670 | 2.3:+774992 | None:intergenic |
| TGATTCAGATCGTAATGGAA+AGG | 0.613377 | 2.3:-774255 | MS.gene86945:CDS |
| CATGTCTGATGATCAAAGAG+AGG | 0.654475 | 2.3:-774183 | MS.gene86945:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGATTATCTAAATTATTT+AGG | + | chr2.3:774348-774367 | None:intergenic | 10.0% |
| !! | TTTATATTTAAATTTGTCAC+AGG | - | chr2.3:775199-775218 | MS.gene86945:intron | 15.0% |
| !!! | ATTTTCTTATATATCTCTTT+TGG | - | chr2.3:775049-775068 | MS.gene86945:CDS | 15.0% |
| !!! | TTTTATGAAAATGGTATATT+TGG | + | chr2.3:773984-774003 | None:intergenic | 15.0% |
| !!! | TTTTATTCTTTATTAGTGTT+TGG | - | chr2.3:774296-774315 | MS.gene86945:intron | 15.0% |
| !!! | TTTTATTGGCTTTTATTTAA+TGG | + | chr2.3:774853-774872 | None:intergenic | 15.0% |
| !! | ACACATTTATTTAATTGAGT+CGG | + | chr2.3:773916-773935 | None:intergenic | 20.0% |
| !! | ACTCAATTAAATAAATGTGT+TGG | - | chr2.3:773916-773935 | MS.gene86945:intron | 20.0% |
| !! | ATCATAAATTAGAAGATTTC+AGG | - | chr2.3:774362-774381 | MS.gene86945:intron | 20.0% |
| !! | GAAATGAAATATTCGTATAT+AGG | + | chr2.3:774831-774850 | None:intergenic | 20.0% |
| !!! | ATGTGTTTACTATTTTATAC+AGG | - | chr2.3:774086-774105 | MS.gene86945:intron | 20.0% |
| !!! | GTTCTTTCTAATTGTAATTT+TGG | - | chr2.3:775154-775173 | MS.gene86945:intron | 20.0% |
| !!! | TGTGGAAAAAAGTTTTTTAT+TGG | + | chr2.3:774867-774886 | None:intergenic | 20.0% |
| !!! | TTTGAATTTTAAAGACTTTG+TGG | - | chr2.3:774184-774203 | MS.gene86945:CDS | 20.0% |
| !!! | TTTTTTTGAAGAAACTCTTT+TGG | - | chr2.3:775084-775103 | MS.gene86945:CDS | 20.0% |
| ! | ACATTTATTTAATTGAGTCG+GGG | + | chr2.3:773914-773933 | None:intergenic | 25.0% |
| ! | ACTATAAGTACCCTAATTAA+AGG | + | chr2.3:774463-774482 | None:intergenic | 25.0% |
| ! | AGAAGAAGAAGAAAAAGATA+AGG | + | chr2.3:773618-773637 | None:intergenic | 25.0% |
| ! | AGTATATCAAACTCAACATA+GGG | + | chr2.3:774738-774757 | None:intergenic | 25.0% |
| ! | ATTCTTTATTAGTGTTTGGA+TGG | - | chr2.3:774300-774319 | MS.gene86945:intron | 25.0% |
| ! | CACATTTATTTAATTGAGTC+GGG | + | chr2.3:773915-773934 | None:intergenic | 25.0% |
| ! | TAGTATATCAAACTCAACAT+AGG | + | chr2.3:774739-774758 | None:intergenic | 25.0% |
| ! | TCAACTAAGTCCTTTAATTA+GGG | - | chr2.3:774450-774469 | MS.gene86945:intron | 25.0% |
| ! | TGTACTAAATGCAGATAAAA+AGG | + | chr2.3:774210-774229 | None:intergenic | 25.0% |
| ! | TTCAACTAAGTCCTTTAATT+AGG | - | chr2.3:774449-774468 | MS.gene86945:intron | 25.0% |
| !! | AAGAATAAAATCTGAAGCAA+TGG | + | chr2.3:774286-774305 | None:intergenic | 25.0% |
| !! | TACAGAAGCATATAGTTATT+TGG | + | chr2.3:774624-774643 | None:intergenic | 25.0% |
| AACATTAAGCAATCAAAGCA+AGG | + | chr2.3:774027-774046 | None:intergenic | 30.0% | |
| AATCATTTATGTATGTCCTG+TGG | - | chr2.3:773587-773606 | MS.gene86945:intron | 30.0% | |
| AGCAAAGTAAGTTTCCATTA+AGG | - | chr2.3:774686-774705 | MS.gene86945:intron | 30.0% | |
| ATTTGATTCAGTTTCTCAAC+AGG | - | chr2.3:773678-773697 | MS.gene86945:intron | 30.0% | |
| GAAGAAGAAAAAGATAAGGA+AGG | + | chr2.3:773614-773633 | None:intergenic | 30.0% | |
| GACATTGGATGATTTCATTA+AGG | - | chr2.3:775019-775038 | MS.gene86945:CDS | 30.0% | |
| GCTAACTCAATATGATATTG+AGG | - | chr2.3:773547-773566 | MS.gene86945:CDS | 30.0% | |
| GTATATGATTCAGATCGTAA+TGG | - | chr2.3:774516-774535 | MS.gene86945:intron | 30.0% | |
| TAACAATTGATGCACCTTAA+TGG | + | chr2.3:774703-774722 | None:intergenic | 30.0% | |
| TTAAATTTGTCACAGGTTCT+TGG | - | chr2.3:775206-775225 | MS.gene86945:CDS | 30.0% | |
| ! | AAAACGTACGTTCAACTTTT+TGG | + | chr2.3:774247-774266 | None:intergenic | 30.0% |
| ! | AAATGGTATATTTGGAACAG+TGG | + | chr2.3:773976-773995 | None:intergenic | 30.0% |
| ! | TTACTGTTCTTTGATTTCTC+AGG | - | chr2.3:774944-774963 | MS.gene86945:intron | 30.0% |
| !! | GAAGTTCTAAAAGATTTGTC+TGG | - | chr2.3:774564-774583 | MS.gene86945:CDS | 30.0% |
| AGAAGATGTCCACTATAAGT+AGG | - | chr2.3:774050-774069 | MS.gene86945:intron | 35.0% | |
| CAACTTGACAGAAATGCTAA+AGG | - | chr2.3:773730-773749 | MS.gene86945:intron | 35.0% | |
| GAAGATGTCCACTATAAGTA+GGG | - | chr2.3:774051-774070 | MS.gene86945:intron | 35.0% | |
| GGTGTCTTTCAAAGACATAT+TGG | - | chr2.3:774542-774561 | MS.gene86945:CDS | 35.0% | |
| GTTTGTGTACTTATTTGCAG+AGG | - | chr2.3:774141-774160 | MS.gene86945:intron | 35.0% | |
| TCTGCATTTAGTACAAAAGC+TGG | - | chr2.3:774215-774234 | MS.gene86945:CDS | 35.0% | |
| TGATATGATACCTGAGAAAG+TGG | + | chr2.3:773805-773824 | None:intergenic | 35.0% | |
| TGTTATGAATCCACTTTCTC+AGG | - | chr2.3:773792-773811 | MS.gene86945:CDS | 35.0% | |
| TTGATTTCTCAGGAAGTCTT+GGG | - | chr2.3:774954-774973 | MS.gene86945:intron | 35.0% | |
| ! | AAAAGACTGCTACTTGACAT+TGG | - | chr2.3:775004-775023 | MS.gene86945:CDS | 35.0% |
| ! | AGTGGATTCATAACAAACTC+AGG | + | chr2.3:773787-773806 | None:intergenic | 35.0% |
| ! | TGATTCAGATCGTAATGGAA+AGG | - | chr2.3:774521-774540 | MS.gene86945:intron | 35.0% |
| ! | TTTGATTTCTCAGGAAGTCT+TGG | - | chr2.3:774953-774972 | MS.gene86945:intron | 35.0% |
| !! | CATCCATTTTTACTCCAGAA+TGG | + | chr2.3:775232-775251 | None:intergenic | 35.0% |
| AAAAGATAAGGAAGGACCAC+AGG | + | chr2.3:773606-773625 | None:intergenic | 40.0% | |
| AGGTTACTCAAGATGGTTGA+TGG | - | chr2.3:774161-774180 | MS.gene86945:intron | 40.0% | |
| CATGTCTGATGATCAAAGAG+AGG | - | chr2.3:774593-774612 | MS.gene86945:CDS | 40.0% | |
| CGAATACAGCACAACTGAAA+AGG | + | chr2.3:774894-774913 | None:intergenic | 40.0% | |
| TATCCTAGCCCTACTTATAG+TGG | + | chr2.3:774062-774081 | None:intergenic | 40.0% | |
| TTTGCAGAGGTTACTCAAGA+TGG | - | chr2.3:774154-774173 | MS.gene86945:intron | 40.0% | |
| !! | TGGCCATTCTGGAGTAAAAA+TGG | - | chr2.3:775226-775245 | MS.gene86945:CDS | 40.0% |
| ATCCTGCTTCTTTCAGAACC+TGG | + | chr2.3:774980-774999 | None:intergenic | 45.0% | |
| TGTCCACTATAAGTAGGGCT+AGG | - | chr2.3:774056-774075 | MS.gene86945:intron | 45.0% | |
| ! | CACAACTGAAAAGGTGTGTG+TGG | + | chr2.3:774885-774904 | None:intergenic | 45.0% |
| !!! | TTTATTTTATTTTATGAAAA+TGG | + | chr2.3:773993-774012 | None:intergenic | 5.0% |
| TCACAGGTTCTTGGCCATTC+TGG | - | chr2.3:775215-775234 | MS.gene86945:CDS | 50.0% | |
| TTCTCAGGAAGTCTTGGGCC+AGG | - | chr2.3:774959-774978 | MS.gene86945:intron | 55.0% | |
| !! | GGCCAGGTTCTGAAAGAAGC+AGG | - | chr2.3:774975-774994 | MS.gene86945:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 773527 | 775271 | 773527 | ID=MS.gene86945 |
| chr2.3 | mRNA | 773527 | 775271 | 773527 | ID=MS.gene86945.t1;Parent=MS.gene86945 |
| chr2.3 | exon | 775202 | 775271 | 775202 | ID=MS.gene86945.t1.exon1;Parent=MS.gene86945.t1 |
| chr2.3 | CDS | 775202 | 775271 | 775202 | ID=cds.MS.gene86945.t1;Parent=MS.gene86945.t1 |
| chr2.3 | exon | 774985 | 775109 | 774985 | ID=MS.gene86945.t1.exon2;Parent=MS.gene86945.t1 |
| chr2.3 | CDS | 774985 | 775109 | 774985 | ID=cds.MS.gene86945.t1;Parent=MS.gene86945.t1 |
| chr2.3 | exon | 774541 | 774637 | 774541 | ID=MS.gene86945.t1.exon3;Parent=MS.gene86945.t1 |
| chr2.3 | CDS | 774541 | 774637 | 774541 | ID=cds.MS.gene86945.t1;Parent=MS.gene86945.t1 |
| chr2.3 | exon | 774184 | 774293 | 774184 | ID=MS.gene86945.t1.exon4;Parent=MS.gene86945.t1 |
| chr2.3 | CDS | 774184 | 774293 | 774184 | ID=cds.MS.gene86945.t1;Parent=MS.gene86945.t1 |
| chr2.3 | exon | 773758 | 773832 | 773758 | ID=MS.gene86945.t1.exon5;Parent=MS.gene86945.t1 |
| chr2.3 | CDS | 773758 | 773832 | 773758 | ID=cds.MS.gene86945.t1;Parent=MS.gene86945.t1 |
| chr2.3 | exon | 773527 | 773577 | 773527 | ID=MS.gene86945.t1.exon6;Parent=MS.gene86945.t1 |
| chr2.3 | CDS | 773527 | 773577 | 773527 | ID=cds.MS.gene86945.t1;Parent=MS.gene86945.t1 |
| Gene Sequence |
| Protein sequence |
