Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87470.t1 | XP_013446394.1 | 95.9 | 123 | 5 | 0 | 1 | 123 | 1 | 123 | 1.70E-59 | 238.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87470.t1 | A0A072TTD9 | 95.9 | 123 | 5 | 0 | 1 | 123 | 1 | 123 | 1.3e-59 | 238.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050883 | MS.gene87470 | 0.813274 | 2.78E-51 | -1.69E-46 |
| MS.gene052091 | MS.gene87470 | 0.812104 | 5.01E-51 | -1.69E-46 |
| MS.gene053826 | MS.gene87470 | 0.815698 | 8.09E-52 | -1.69E-46 |
| MS.gene055549 | MS.gene87470 | 0.805294 | 1.43E-49 | -1.69E-46 |
| MS.gene055550 | MS.gene87470 | 0.804112 | 2.53E-49 | -1.69E-46 |
| MS.gene055551 | MS.gene87470 | 0.826392 | 2.78E-54 | -1.69E-46 |
| MS.gene055961 | MS.gene87470 | 0.80329 | 3.74E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87470.t1 | MTR_8g077145 | 95.935 | 123 | 5 | 0 | 1 | 123 | 1 | 123 | 1.12e-84 | 243 |
| MS.gene87470.t1 | MTR_5g020860 | 61.417 | 127 | 43 | 3 | 1 | 123 | 1 | 125 | 1.72e-47 | 149 |
| MS.gene87470.t1 | MTR_7g026910 | 43.956 | 91 | 28 | 4 | 1 | 89 | 1 | 70 | 8.10e-12 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87470.t1 | AT2G18210 | 46.875 | 128 | 57 | 6 | 1 | 123 | 3 | 124 | 2.85e-25 | 92.8 |
| MS.gene87470.t1 | AT4G36500 | 44.000 | 125 | 63 | 3 | 1 | 123 | 3 | 122 | 2.07e-21 | 82.8 |
| MS.gene87470.t1 | AT3G50900 | 40.602 | 133 | 50 | 6 | 1 | 123 | 1 | 114 | 2.79e-21 | 82.4 |
| MS.gene87470.t1 | AT5G66490 | 36.923 | 130 | 54 | 6 | 1 | 123 | 1 | 109 | 7.14e-14 | 63.2 |
Find 26 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAATAGATAACTGGTTTAT+TGG | 0.180498 | 8.3:-26962551 | None:intergenic |
| ATGACTCTAGGTTCAACTTT+TGG | 0.219816 | 8.3:+26962355 | MS.gene87470:CDS |
| TCATCCACTTCTCAACTTCT+TGG | 0.245054 | 8.3:+26962438 | MS.gene87470:CDS |
| TCAATATACCTTGCAAATTC+AGG | 0.324241 | 8.3:-26962493 | None:intergenic |
| TATCACTTGTAATAGATAAC+TGG | 0.340382 | 8.3:-26962559 | None:intergenic |
| TTACTATTAGCCTTCTTTGT+GGG | 0.364970 | 8.3:-26962400 | None:intergenic |
| TTGACAACTCGGAGAGGCCT+TGG | 0.364992 | 8.3:+26962220 | MS.gene87470:CDS |
| ACTCGGAGAGGCCTTGGAAA+TGG | 0.385033 | 8.3:+26962226 | MS.gene87470:CDS |
| ATTACTATTAGCCTTCTTTG+TGG | 0.397888 | 8.3:-26962401 | None:intergenic |
| CTCGGAGAGGCCTTGGAAAT+GGG | 0.438584 | 8.3:+26962227 | MS.gene87470:CDS |
| AAGGTATATTGAGTATCTTA+AGG | 0.468319 | 8.3:+26962504 | MS.gene87470:CDS |
| ATGGGAGATATGAGAGACTA+GGG | 0.472587 | 8.3:+26962245 | MS.gene87470:CDS |
| TACTATTAGCCTTCTTTGTG+GGG | 0.481965 | 8.3:-26962399 | None:intergenic |
| GAAGTATTGGATGCTCTAGT+AGG | 0.500496 | 8.3:-26962325 | None:intergenic |
| AATGGGAGATATGAGAGACT+AGG | 0.509338 | 8.3:+26962244 | MS.gene87470:CDS |
| TATATTGAGTATCTTAAGGA+AGG | 0.518072 | 8.3:+26962508 | MS.gene87470:CDS |
| AAATCCAAGAAGTTGAGAAG+TGG | 0.550501 | 8.3:-26962442 | None:intergenic |
| TGTTTAGATCTTTGACAACT+CGG | 0.569650 | 8.3:+26962209 | MS.gene87470:CDS |
| ATCTTAAGGAAGGAGGCATG+TGG | 0.588582 | 8.3:+26962518 | MS.gene87470:CDS |
| TCTCATATCTCCCATTTCCA+AGG | 0.611655 | 8.3:-26962237 | None:intergenic |
| CAGCTAGGCCTGAATTTGCA+AGG | 0.622241 | 8.3:+26962485 | MS.gene87470:CDS |
| ATTGAGTATCTTAAGGAAGG+AGG | 0.641255 | 8.3:+26962511 | MS.gene87470:CDS |
| CTCATCATGCAAAAGTGTTG+TGG | 0.641475 | 8.3:-26962281 | None:intergenic |
| TCTTAAGGAAGGAGGCATGT+GGG | 0.644106 | 8.3:+26962519 | MS.gene87470:CDS |
| AGATCTTTGACAACTCGGAG+AGG | 0.670905 | 8.3:+26962214 | MS.gene87470:CDS |
| AGTTGTCAAAGATCTAAACA+TGG | 0.718860 | 8.3:-26962206 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGGTATATTGAGTATCTTA+AGG | + | chr8.3:26962504-26962523 | MS.gene87470:CDS | 25.0% |
| ! | TATATTGAGTATCTTAAGGA+AGG | + | chr8.3:26962508-26962527 | MS.gene87470:CDS | 25.0% |
| !! | GTAATAGATAACTGGTTTAT+TGG | - | chr8.3:26962554-26962573 | None:intergenic | 25.0% |
| !!! | AGTCATTTTTCTTGAAGTAT+TGG | - | chr8.3:26962341-26962360 | None:intergenic | 25.0% |
| ACTTCAAGAAAAATGACTCT+AGG | + | chr8.3:26962343-26962362 | MS.gene87470:CDS | 30.0% | |
| TCAATATACCTTGCAAATTC+AGG | - | chr8.3:26962496-26962515 | None:intergenic | 30.0% | |
| TGTTTAGATCTTTGACAACT+CGG | + | chr8.3:26962209-26962228 | MS.gene87470:CDS | 30.0% | |
| !! | ATTACTATTAGCCTTCTTTG+TGG | - | chr8.3:26962404-26962423 | None:intergenic | 30.0% |
| !! | TTACTATTAGCCTTCTTTGT+GGG | - | chr8.3:26962403-26962422 | None:intergenic | 30.0% |
| AAAAAGAAACCCCACAAAGA+AGG | + | chr8.3:26962390-26962409 | MS.gene87470:CDS | 35.0% | |
| AAATCCAAGAAGTTGAGAAG+TGG | - | chr8.3:26962445-26962464 | None:intergenic | 35.0% | |
| ATTGAGTATCTTAAGGAAGG+AGG | + | chr8.3:26962511-26962530 | MS.gene87470:CDS | 35.0% | |
| GGAAAAAGAAAACAACAGCT+AGG | + | chr8.3:26962470-26962489 | MS.gene87470:CDS | 35.0% | |
| ! | ATGACTCTAGGTTCAACTTT+TGG | + | chr8.3:26962355-26962374 | MS.gene87470:CDS | 35.0% |
| ! | TCAACTTCTTGGATTTTCGT+AGG | + | chr8.3:26962449-26962468 | MS.gene87470:CDS | 35.0% |
| ! | TCTTTGTGGGGTTTCTTTTT+AGG | - | chr8.3:26962390-26962409 | None:intergenic | 35.0% |
| ! | TGACTCTAGGTTCAACTTTT+GGG | + | chr8.3:26962356-26962375 | MS.gene87470:CDS | 35.0% |
| !! | TACTATTAGCCTTCTTTGTG+GGG | - | chr8.3:26962402-26962421 | None:intergenic | 35.0% |
| AATGGGAGATATGAGAGACT+AGG | + | chr8.3:26962244-26962263 | MS.gene87470:CDS | 40.0% | |
| ATGGGAGATATGAGAGACTA+GGG | + | chr8.3:26962245-26962264 | MS.gene87470:CDS | 40.0% | |
| TCATCCACTTCTCAACTTCT+TGG | + | chr8.3:26962438-26962457 | MS.gene87470:CDS | 40.0% | |
| TCTCATATCTCCCATTTCCA+AGG | - | chr8.3:26962240-26962259 | None:intergenic | 40.0% | |
| ! | GACTCTAGGTTCAACTTTTG+GGG | + | chr8.3:26962357-26962376 | MS.gene87470:CDS | 40.0% |
| !! | CTCATCATGCAAAAGTGTTG+TGG | - | chr8.3:26962284-26962303 | None:intergenic | 40.0% |
| !! | GAAGTATTGGATGCTCTAGT+AGG | - | chr8.3:26962328-26962347 | None:intergenic | 40.0% |
| AGATCTTTGACAACTCGGAG+AGG | + | chr8.3:26962214-26962233 | MS.gene87470:CDS | 45.0% | |
| ! | ATCTTAAGGAAGGAGGCATG+TGG | + | chr8.3:26962518-26962537 | MS.gene87470:CDS | 45.0% |
| ! | TCTTAAGGAAGGAGGCATGT+GGG | + | chr8.3:26962519-26962538 | MS.gene87470:CDS | 45.0% |
| CAGCTAGGCCTGAATTTGCA+AGG | + | chr8.3:26962485-26962504 | MS.gene87470:CDS | 50.0% | |
| ACTCGGAGAGGCCTTGGAAA+TGG | + | chr8.3:26962226-26962245 | MS.gene87470:CDS | 55.0% | |
| CTCGGAGAGGCCTTGGAAAT+GGG | + | chr8.3:26962227-26962246 | MS.gene87470:CDS | 55.0% | |
| TTGACAACTCGGAGAGGCCT+TGG | + | chr8.3:26962220-26962239 | MS.gene87470:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 26962208 | 26962579 | 26962208 | ID=MS.gene87470 |
| chr8.3 | mRNA | 26962208 | 26962579 | 26962208 | ID=MS.gene87470.t1;Parent=MS.gene87470 |
| chr8.3 | exon | 26962208 | 26962579 | 26962208 | ID=MS.gene87470.t1.exon1;Parent=MS.gene87470.t1 |
| chr8.3 | CDS | 26962208 | 26962579 | 26962208 | ID=cds.MS.gene87470.t1;Parent=MS.gene87470.t1 |
| Gene Sequence |
| Protein sequence |
