Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88024.t1 | XP_004492881.1 | 40.1 | 177 | 76 | 3 | 25 | 172 | 74 | 249 | 2.20E-16 | 95.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88024.t1 | A0A392LY79 | 48.9 | 182 | 73 | 4 | 12 | 181 | 203 | 376 | 5.1e-31 | 144.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene88024 | -0.809569 | 1.77E-50 | -1.69E-46 |
| MS.gene050226 | MS.gene88024 | -0.811471 | 6.89E-51 | -1.69E-46 |
| MS.gene050227 | MS.gene88024 | -0.816151 | 6.41E-52 | -1.69E-46 |
| MS.gene050228 | MS.gene88024 | -0.82753 | 1.49E-54 | -1.69E-46 |
| MS.gene050330 | MS.gene88024 | 0.811489 | 6.83E-51 | -1.69E-46 |
| MS.gene050623 | MS.gene88024 | 0.828949 | 6.76E-55 | -1.69E-46 |
| MS.gene050807 | MS.gene88024 | -0.827212 | 1.77E-54 | -1.69E-46 |
| MS.gene050987 | MS.gene88024 | -0.806516 | 7.92E-50 | -1.69E-46 |
| MS.gene051243 | MS.gene88024 | -0.800343 | 1.51E-48 | -1.69E-46 |
| MS.gene052176 | MS.gene88024 | -0.817393 | 3.37E-52 | -1.69E-46 |
| MS.gene052309 | MS.gene88024 | 0.815973 | 7.02E-52 | -1.69E-46 |
| MS.gene052323 | MS.gene88024 | -0.805489 | 1.30E-49 | -1.69E-46 |
| MS.gene053085 | MS.gene88024 | -0.804866 | 1.76E-49 | -1.69E-46 |
| MS.gene054942 | MS.gene88024 | -0.814293 | 1.66E-51 | -1.69E-46 |
| MS.gene056069 | MS.gene88024 | 0.810938 | 8.99E-51 | -1.69E-46 |
| MS.gene056760 | MS.gene88024 | -0.809537 | 1.80E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene88024 | -0.835931 | 1.26E-56 | -1.69E-46 |
| MS.gene059569 | MS.gene88024 | -0.829745 | 4.33E-55 | -1.69E-46 |
| MS.gene060572 | MS.gene88024 | -0.80697 | 6.35E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88024.t1 | MTR_7g081150 | 42.000 | 100 | 49 | 2 | 53 | 152 | 212 | 302 | 2.07e-13 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGTGATATGGCATGCTTTC+GGG | 0.197077 | 4.3:-69765349 | MS.gene88024:CDS |
| AGAAATCTATGCTTATCTTT+TGG | 0.235647 | 4.3:-69765442 | MS.gene88024:CDS |
| CTGGCAGATTGGCAACTTAT+TGG | 0.248113 | 4.3:+69765286 | None:intergenic |
| TCCTTGCACTTCTAGTGATA+TGG | 0.294087 | 4.3:-69765361 | MS.gene88024:CDS |
| CTAGTGATATGGCATGCTTT+CGG | 0.382639 | 4.3:-69765350 | MS.gene88024:CDS |
| CCGTCCTTGCTCAGGTTGTC+CGG | 0.394563 | 4.3:-69765530 | MS.gene88024:CDS |
| TCTTTCTCTGCGTGATAGAT+TGG | 0.399237 | 4.3:+69765761 | None:intergenic |
| AGTAAGGTTTCAAGGTCTAT+TGG | 0.404978 | 4.3:+69765785 | None:intergenic |
| TGATTGAAGATGCAACAAAA+TGG | 0.411390 | 4.3:+69765418 | None:intergenic |
| ATGTGCAGTCCATTGTTATC+CGG | 0.415512 | 4.3:+69765701 | None:intergenic |
| TACCTGCAATGAAGATGTTT+CGG | 0.417070 | 4.3:-69766064 | None:intergenic |
| CCATATGGCCGTCCTTGCTC+AGG | 0.421763 | 4.3:-69765538 | MS.gene88024:CDS |
| TGCGTGATAGATTGGAAGTA+AGG | 0.424769 | 4.3:+69765769 | None:intergenic |
| TTATTGATCACATCTTCATC+TGG | 0.432963 | 4.3:+69765314 | None:intergenic |
| CCTGAGCAAGGACGGCCATA+TGG | 0.435865 | 4.3:+69765538 | None:intergenic |
| ACAAATAAACACCATAAAAC+CGG | 0.436981 | 4.3:+69765511 | None:intergenic |
| TATGCTGCTCCATATGTTGC+AGG | 0.452102 | 4.3:+69765613 | None:intergenic |
| TATTTGTGCCGTTGCTGCAA+TGG | 0.492504 | 4.3:-69765495 | MS.gene88024:CDS |
| AGATTGGAAGTAAGGTTTCA+AGG | 0.495918 | 4.3:+69765777 | None:intergenic |
| TTGATCACATCTTCATCTGG+TGG | 0.516218 | 4.3:+69765317 | None:intergenic |
| CCGGACAACCTGAGCAAGGA+CGG | 0.520130 | 4.3:+69765530 | None:intergenic |
| GGGATGACCGCCTTTCCATA+TGG | 0.520131 | 4.3:-69765553 | MS.gene88024:CDS |
| ATGTAACATGTTGTCCCTCC+CGG | 0.524389 | 4.3:-69765720 | MS.gene88024:CDS |
| GACAAGAACCATTGCAGCAA+CGG | 0.526821 | 4.3:+69765487 | None:intergenic |
| TGTGCAGTCCATTGTTATCC+GGG | 0.548984 | 4.3:+69765702 | None:intergenic |
| AGGTTTCAAGGTCTATTGGT+AGG | 0.553409 | 4.3:+69765789 | None:intergenic |
| GTGCATAAGATGAAAGCCAT+TGG | 0.555210 | 4.3:+69765663 | None:intergenic |
| ACGCAGAGAAAGACAATCAA+AGG | 0.571451 | 4.3:-69765751 | MS.gene88024:CDS |
| CAGTCCATTGTTATCCGGGA+GGG | 0.596015 | 4.3:+69765706 | None:intergenic |
| GATTTCTGCATACTCGTGAA+AGG | 0.607818 | 4.3:+69765458 | None:intergenic |
| GCAAGGACGGCCATATGGAA+AGG | 0.623069 | 4.3:+69765543 | None:intergenic |
| TTGTCCCTCCCGGATAACAA+TGG | 0.623917 | 4.3:-69765710 | MS.gene88024:CDS |
| TGCGTATGAAAATCATCCAA+TGG | 0.624324 | 4.3:-69765679 | MS.gene88024:CDS |
| AAAACCGGACAACCTGAGCA+AGG | 0.646626 | 4.3:+69765526 | None:intergenic |
| GCATACTCGTGAAAGGTGCA+CGG | 0.652146 | 4.3:+69765465 | None:intergenic |
| GCCATATCACTAGAAGTGCA+AGG | 0.653658 | 4.3:+69765360 | None:intergenic |
| AGGACGGCCATATGGAAAGG+CGG | 0.693360 | 4.3:+69765546 | None:intergenic |
| GCAGTCCATTGTTATCCGGG+AGG | 0.697924 | 4.3:+69765705 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTTATAACCTTCATGA+TGG | - | chr4.3:69765312-69765331 | MS.gene88024:CDS | 20.0% |
| ! | AAATTCATACATAGCATAGA+GGG | + | chr4.3:69765498-69765517 | None:intergenic | 25.0% |
| ! | ACAAATAAACACCATAAAAC+CGG | + | chr4.3:69765825-69765844 | None:intergenic | 25.0% |
| ! | ACAAATAGAAGTCAAATTAG+AGG | + | chr4.3:69765399-69765418 | None:intergenic | 25.0% |
| ! | CAAATAGAAGTCAAATTAGA+GGG | + | chr4.3:69765398-69765417 | None:intergenic | 25.0% |
| ! | TGAAAAAATATCCATCATGA+AGG | + | chr4.3:69765326-69765345 | None:intergenic | 25.0% |
| ! | TTCTATTTGTGTTATGATGT+AGG | - | chr4.3:69765409-69765428 | MS.gene88024:CDS | 25.0% |
| !! | AGAAATCTATGCTTATCTTT+TGG | - | chr4.3:69765891-69765910 | MS.gene88024:intron | 25.0% |
| !! | GAAATCTATGCTTATCTTTT+GGG | - | chr4.3:69765892-69765911 | MS.gene88024:intron | 25.0% |
| !!! | TTTTTATTTCCTGCAACATA+TGG | - | chr4.3:69765711-69765730 | MS.gene88024:CDS | 25.0% |
| AATTCATACATAGCATAGAG+GGG | + | chr4.3:69765497-69765516 | None:intergenic | 30.0% | |
| GAAATTCATACATAGCATAG+AGG | + | chr4.3:69765499-69765518 | None:intergenic | 30.0% | |
| GATGACATTTATCATTCCTT+TGG | - | chr4.3:69765372-69765391 | MS.gene88024:CDS | 30.0% | |
| GGAAATAAAAACAAGGAGAT+TGG | + | chr4.3:69765702-69765721 | None:intergenic | 30.0% | |
| TGATTGAAGATGCAACAAAA+TGG | + | chr4.3:69765918-69765937 | None:intergenic | 30.0% | |
| TGTTGCAGGAAATAAAAACA+AGG | + | chr4.3:69765709-69765728 | None:intergenic | 30.0% | |
| TTATTGATCACATCTTCATC+TGG | + | chr4.3:69766022-69766041 | None:intergenic | 30.0% | |
| AGATTGGAAGTAAGGTTTCA+AGG | + | chr4.3:69765559-69765578 | None:intergenic | 35.0% | |
| AGTAAGGTTTCAAGGTCTAT+TGG | + | chr4.3:69765551-69765570 | None:intergenic | 35.0% | |
| CGGTAACTTAACAATTACTC+TGG | - | chr4.3:69765289-69765308 | MS.gene88024:CDS | 35.0% | |
| TGCGTATGAAAATCATCCAA+TGG | - | chr4.3:69765654-69765673 | MS.gene88024:CDS | 35.0% | |
| AAGTCAAATTAGAGGGCCAA+AGG | + | chr4.3:69765391-69765410 | None:intergenic | 40.0% | |
| ACGCAGAGAAAGACAATCAA+AGG | - | chr4.3:69765582-69765601 | MS.gene88024:CDS | 40.0% | |
| AGGTTTCAAGGTCTATTGGT+AGG | + | chr4.3:69765547-69765566 | None:intergenic | 40.0% | |
| ATGTGCAGTCCATTGTTATC+CGG | + | chr4.3:69765635-69765654 | None:intergenic | 40.0% | |
| CTAGTGATATGGCATGCTTT+CGG | - | chr4.3:69765983-69766002 | MS.gene88024:intron | 40.0% | |
| GATTTCTGCATACTCGTGAA+AGG | + | chr4.3:69765878-69765897 | None:intergenic | 40.0% | |
| GTGCATAAGATGAAAGCCAT+TGG | + | chr4.3:69765673-69765692 | None:intergenic | 40.0% | |
| TAGTGATATGGCATGCTTTC+GGG | - | chr4.3:69765984-69766003 | MS.gene88024:intron | 40.0% | |
| TCCTTGCACTTCTAGTGATA+TGG | - | chr4.3:69765972-69765991 | MS.gene88024:intron | 40.0% | |
| TCTTTCTCTGCGTGATAGAT+TGG | + | chr4.3:69765575-69765594 | None:intergenic | 40.0% | |
| TGCGTGATAGATTGGAAGTA+AGG | + | chr4.3:69765567-69765586 | None:intergenic | 40.0% | |
| TTGATCACATCTTCATCTGG+TGG | + | chr4.3:69766019-69766038 | None:intergenic | 40.0% | |
| !!! | TCATCCCATTTTTTGTGGAG+TGG | + | chr4.3:69765767-69765786 | None:intergenic | 40.0% |
| ACATAGCATAGAGGGGTCAA+TGG | + | chr4.3:69765490-69765509 | None:intergenic | 45.0% | |
| ATGTAACATGTTGTCCCTCC+CGG | - | chr4.3:69765613-69765632 | MS.gene88024:CDS | 45.0% | |
| CCCTCCACTCCACAAAAAAT+GGG | - | chr4.3:69765760-69765779 | MS.gene88024:CDS | 45.0% | |
| CTGGCAGATTGGCAACTTAT+TGG | + | chr4.3:69766050-69766069 | None:intergenic | 45.0% | |
| GACAAGAACCATTGCAGCAA+CGG | + | chr4.3:69765849-69765868 | None:intergenic | 45.0% | |
| TATGCTGCTCCATATGTTGC+AGG | + | chr4.3:69765723-69765742 | None:intergenic | 45.0% | |
| TCCCTCCACTCCACAAAAAA+TGG | - | chr4.3:69765759-69765778 | MS.gene88024:CDS | 45.0% | |
| TGTGCAGTCCATTGTTATCC+GGG | + | chr4.3:69765634-69765653 | None:intergenic | 45.0% | |
| ! | GCCATATCACTAGAAGTGCA+AGG | + | chr4.3:69765976-69765995 | None:intergenic | 45.0% |
| ! | TATTTGTGCCGTTGCTGCAA+TGG | - | chr4.3:69765838-69765857 | MS.gene88024:intron | 45.0% |
| !!! | CCCATTTTTTGTGGAGTGGA+GGG | + | chr4.3:69765763-69765782 | None:intergenic | 45.0% |
| !!! | TCCCATTTTTTGTGGAGTGG+AGG | + | chr4.3:69765764-69765783 | None:intergenic | 45.0% |
| AAAACCGGACAACCTGAGCA+AGG | + | chr4.3:69765810-69765829 | None:intergenic | 50.0% | |
| CAGTCCATTGTTATCCGGGA+GGG | + | chr4.3:69765630-69765649 | None:intergenic | 50.0% | |
| TTGTCCCTCCCGGATAACAA+TGG | - | chr4.3:69765623-69765642 | MS.gene88024:CDS | 50.0% | |
| ! | GCATACTCGTGAAAGGTGCA+CGG | + | chr4.3:69765871-69765890 | None:intergenic | 50.0% |
| ! | TTTTGTGGAGTGGAGGGACA+TGG | + | chr4.3:69765757-69765776 | None:intergenic | 50.0% |
| !! | GCTCAGGTTGTCCGGTTTTA+TGG | - | chr4.3:69765811-69765830 | MS.gene88024:CDS | 50.0% |
| !!! | GGCGGTCATCCCATTTTTTG+TGG | + | chr4.3:69765772-69765791 | None:intergenic | 50.0% |
| AGGACGGCCATATGGAAAGG+CGG | + | chr4.3:69765790-69765809 | None:intergenic | 55.0% | |
| GCAAGGACGGCCATATGGAA+AGG | + | chr4.3:69765793-69765812 | None:intergenic | 55.0% | |
| GCAGTCCATTGTTATCCGGG+AGG | + | chr4.3:69765631-69765650 | None:intergenic | 55.0% | |
| GGGATGACCGCCTTTCCATA+TGG | - | chr4.3:69765780-69765799 | MS.gene88024:CDS | 55.0% | |
| CCATATGGCCGTCCTTGCTC+AGG | - | chr4.3:69765795-69765814 | MS.gene88024:CDS | 60.0% | |
| CCGGACAACCTGAGCAAGGA+CGG | + | chr4.3:69765806-69765825 | None:intergenic | 60.0% | |
| CCTGAGCAAGGACGGCCATA+TGG | + | chr4.3:69765798-69765817 | None:intergenic | 60.0% | |
| ! | CCGTCCTTGCTCAGGTTGTC+CGG | - | chr4.3:69765803-69765822 | MS.gene88024:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 69765277 | 69766078 | 69765277 | ID=MS.gene88024 |
| chr4.3 | mRNA | 69765277 | 69766078 | 69765277 | ID=MS.gene88024.t1;Parent=MS.gene88024 |
| chr4.3 | exon | 69766065 | 69766078 | 69766065 | ID=MS.gene88024.t1.exon1;Parent=MS.gene88024.t1 |
| chr4.3 | CDS | 69766065 | 69766078 | 69766065 | ID=cds.MS.gene88024.t1;Parent=MS.gene88024.t1 |
| chr4.3 | exon | 69765912 | 69765924 | 69765912 | ID=MS.gene88024.t1.exon2;Parent=MS.gene88024.t1 |
| chr4.3 | CDS | 69765912 | 69765924 | 69765912 | ID=cds.MS.gene88024.t1;Parent=MS.gene88024.t1 |
| chr4.3 | exon | 69765277 | 69765834 | 69765277 | ID=MS.gene88024.t1.exon3;Parent=MS.gene88024.t1 |
| chr4.3 | CDS | 69765277 | 69765834 | 69765277 | ID=cds.MS.gene88024.t1;Parent=MS.gene88024.t1 |
| Gene Sequence |
| Protein sequence |
