AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056760


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056760.t1 MTR_8g031320 100.000 72 0 0 1 72 1 72 2.15e-50 153
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056760.t1 AT5G44710 59.722 72 29 0 1 72 1 72 6.10e-31 104

Find 16 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCATCAATTGAATCCTTC+AGG 0.310963 8.4:-65948778 MS.gene056760:CDS
ATTCTCCGTATGAAAATGTT+TGG 0.383146 8.4:-65948736 MS.gene056760:CDS
TGTTGACGAGCCATAACATT+GGG 0.413822 8.4:+65948667 None:intergenic
TGAAGATGACCCCAATGTTA+TGG 0.423766 8.4:-65948677 MS.gene056760:CDS
TCTGCGCGAGCAACAATATT+CGG 0.425555 8.4:-65948799 MS.gene056760:CDS
GTCATCAATTGAATCCTTCA+GGG 0.500225 8.4:-65948777 MS.gene056760:CDS
ATGTTAACCTCTGCAATCAA+TGG 0.543107 8.4:-65948832 MS.gene056760:CDS
TGAGCAGATTTCTTCCCTGA+AGG 0.553711 8.4:+65948763 None:intergenic
TCATCTTCATTGATATCATG+AGG 0.573272 8.4:+65948691 None:intergenic
TTAACCTCTGCAATCAATGG+TGG 0.636449 8.4:-65948829 MS.gene056760:CDS
TTGTTGACGAGCCATAACAT+TGG 0.639191 8.4:+65948666 None:intergenic
CATCTTCATTGATATCATGA+GGG 0.654939 8.4:+65948692 None:intergenic
GAGACCACCATTGATTGCAG+AGG 0.678273 8.4:+65948825 None:intergenic
GTTGACGAGCCATAACATTG+GGG 0.685036 8.4:+65948668 None:intergenic
AAGAAGAAGATGGCTAGCGG+CGG 0.707252 8.4:-65948865 None:intergenic
GTAAAGAAGAAGATGGCTAG+CGG 0.708935 8.4:-65948868 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCACCAAACATTTTCATA+CGG + chr8.4:65948785-65948804 None:intergenic 25.0%
ATTCTCCGTATGAAAATGTT+TGG - chr8.4:65948777-65948796 MS.gene056760:CDS 30.0%
CATCTTCATTGATATCATGA+GGG + chr8.4:65948824-65948843 None:intergenic 30.0%
TCATCTTCATTGATATCATG+AGG + chr8.4:65948825-65948844 None:intergenic 30.0%
ATGTTAACCTCTGCAATCAA+TGG - chr8.4:65948681-65948700 MS.gene056760:CDS 35.0%
GTCATCAATTGAATCCTTCA+GGG - chr8.4:65948736-65948755 MS.gene056760:CDS 35.0%
TTGGTGAAAAAGTTGCTCAA+TGG - chr8.4:65948796-65948815 MS.gene056760:CDS 35.0%
GGTCATCAATTGAATCCTTC+AGG - chr8.4:65948735-65948754 MS.gene056760:CDS 40.0%
TGAAGATGACCCCAATGTTA+TGG - chr8.4:65948836-65948855 MS.gene056760:CDS 40.0%
TGTTGACGAGCCATAACATT+GGG + chr8.4:65948849-65948868 None:intergenic 40.0%
TTAACCTCTGCAATCAATGG+TGG - chr8.4:65948684-65948703 MS.gene056760:CDS 40.0%
TTGTTGACGAGCCATAACAT+TGG + chr8.4:65948850-65948869 None:intergenic 40.0%
GTTGACGAGCCATAACATTG+GGG + chr8.4:65948848-65948867 None:intergenic 45.0%
TCTGCGCGAGCAACAATATT+CGG - chr8.4:65948714-65948733 MS.gene056760:CDS 45.0%
TGAGCAGATTTCTTCCCTGA+AGG + chr8.4:65948753-65948772 None:intergenic 45.0%
! GAGACCACCATTGATTGCAG+AGG + chr8.4:65948691-65948710 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 65948657 65948878 65948657 ID=MS.gene056760
chr8.4 mRNA 65948657 65948878 65948657 ID=MS.gene056760.t1;Parent=MS.gene056760
chr8.4 exon 65948657 65948878 65948657 ID=MS.gene056760.t1.exon1;Parent=MS.gene056760.t1
chr8.4 CDS 65948657 65948878 65948657 ID=cds.MS.gene056760.t1;Parent=MS.gene056760.t1
Gene Sequence

>MS.gene056760

ATGGCTAGCGGCGGTTTGAAGAAGATGTTAACCTCTGCAATCAATGGTGGTCTCACTTCTGCGCGAGCAACAATATTCGGTCATCAATTGAATCCTTCAGGGAAGAAATCTGCTCACAAAATTCTCCGTATGAAAATGTTTGGTGAAAAAGTTGCTCAATGGTACCCTCATGATATCAATGAAGATGACCCCAATGTTATGGCTCGTCAACAACAAGTGTAA

Protein sequence

>MS.gene056760.t1

MASGGLKKMLTSAINGGLTSARATIFGHQLNPSGKKSAHKILRMKMFGEKVAQWYPHDINEDDPNVMARQQQV