Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059569.t1 | XP_013453160.1 | 99.1 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 1.80E-56 | 228.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059569.t1 | B0TE72 | 30.9 | 97 | 62 | 2 | 1 | 96 | 1 | 93 | 4.3e-06 | 52.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059569.t1 | A0A072UDJ5 | 99.1 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 1.3e-56 | 228.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050303 | MS.gene059569 | 0.80149 | 8.78E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene059569 | 0.80015 | 1.65E-48 | -1.69E-46 |
| MS.gene050750 | MS.gene059569 | 0.805002 | 1.65E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene059569 | 0.804773 | 1.84E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene059569 | 0.803242 | 3.83E-49 | -1.69E-46 |
| MS.gene050987 | MS.gene059569 | 0.804333 | 2.27E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene059569 | 0.817851 | 2.66E-52 | -1.69E-46 |
| MS.gene052302 | MS.gene059569 | 0.811931 | 5.47E-51 | -1.69E-46 |
| MS.gene052309 | MS.gene059569 | -0.819751 | 9.85E-53 | -1.69E-46 |
| MS.gene052376 | MS.gene059569 | 0.820119 | 8.11E-53 | -1.69E-46 |
| MS.gene053080 | MS.gene059569 | 0.809136 | 2.20E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene059569 | 0.802061 | 6.71E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene059569 | 0.807694 | 4.46E-50 | -1.69E-46 |
| MS.gene054657 | MS.gene059569 | 0.804552 | 2.05E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene059569 | 0.802735 | 4.87E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene059569 | 0.831723 | 1.42E-55 | -1.69E-46 |
| MS.gene057079 | MS.gene059569 | 0.805876 | 1.08E-49 | -1.69E-46 |
| MS.gene057414 | MS.gene059569 | 0.80408 | 2.57E-49 | -1.69E-46 |
| MS.gene057555 | MS.gene059569 | 0.817001 | 4.13E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene059569 | 0.835211 | 1.91E-56 | -1.69E-46 |
| MS.gene059569 | MS.gene05979 | 0.812846 | 3.45E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene060429 | 0.823052 | 1.70E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene060572 | 0.8617 | 8.53E-64 | -1.69E-46 |
| MS.gene059569 | MS.gene060803 | 0.804311 | 2.30E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene060907 | 0.810291 | 1.24E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene061719 | 0.813241 | 2.83E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene063534 | -0.818545 | 1.85E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene063917 | 0.805549 | 1.27E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene064185 | 0.802661 | 5.05E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene064462 | 0.806785 | 6.95E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene064797 | -0.814758 | 1.31E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene064937 | 0.834173 | 3.49E-56 | -1.69E-46 |
| MS.gene059569 | MS.gene064944 | 0.838136 | 3.44E-57 | -1.69E-46 |
| MS.gene059569 | MS.gene066194 | 0.814888 | 1.22E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene066264 | 0.800783 | 1.23E-48 | -1.69E-46 |
| MS.gene059569 | MS.gene066320 | 0.808283 | 3.34E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene06667 | 0.80624 | 9.05E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene06705 | 0.809714 | 1.65E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene067151 | 0.80367 | 3.12E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene067932 | 0.80339 | 3.57E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene068385 | 0.816737 | 4.74E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene068549 | 0.801459 | 8.92E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene068637 | 0.804544 | 2.05E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene069206 | 0.81465 | 1.38E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene06921 | 0.801836 | 7.46E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene06922 | 0.807602 | 4.67E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene07084 | 0.804084 | 2.56E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene070899 | 0.827713 | 1.34E-54 | -1.69E-46 |
| MS.gene059569 | MS.gene070901 | 0.823293 | 1.50E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene07115 | 0.805763 | 1.14E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene071570 | 0.834717 | 2.55E-56 | -1.69E-46 |
| MS.gene059569 | MS.gene072087 | -0.809624 | 1.73E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene07344 | 0.808138 | 3.59E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene07368 | 0.822432 | 2.38E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene073833 | 0.805355 | 1.39E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene073954 | 0.835327 | 1.79E-56 | -1.69E-46 |
| MS.gene059569 | MS.gene074527 | 0.80341 | 3.53E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene07882 | 0.807672 | 4.51E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene08324 | -0.808226 | 3.44E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene08485 | 0.80963 | 1.72E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene08618 | 0.805625 | 1.22E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene08701 | 0.823855 | 1.11E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene08782 | 0.804906 | 1.72E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene09952 | 0.814642 | 1.39E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene20438 | 0.804817 | 1.80E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene20912 | 0.821234 | 4.50E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene21134 | 0.800655 | 1.30E-48 | -1.69E-46 |
| MS.gene059569 | MS.gene21149 | 0.825554 | 4.40E-54 | -1.69E-46 |
| MS.gene059569 | MS.gene21783 | 0.801234 | 9.91E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene21881 | 0.801212 | 1.00E-48 | -1.69E-46 |
| MS.gene059569 | MS.gene22270 | 0.800774 | 1.23E-48 | -1.69E-46 |
| MS.gene059569 | MS.gene22314 | 0.802173 | 6.36E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene22691 | 0.810396 | 1.18E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene22820 | -0.808928 | 2.43E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene23105 | 0.806069 | 9.84E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene23546 | 0.83694 | 6.97E-57 | -1.69E-46 |
| MS.gene059569 | MS.gene24012 | 0.810946 | 8.95E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene24692 | 0.813728 | 2.21E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene25207 | 0.828321 | 9.59E-55 | -1.69E-46 |
| MS.gene059569 | MS.gene25259 | 0.808475 | 3.04E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene26666 | 0.812596 | 3.92E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene27482 | 0.817662 | 2.94E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene28118 | 0.802975 | 4.35E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene28218 | 0.818318 | 2.09E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene28498 | 0.813932 | 1.99E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene28864 | 0.818029 | 2.43E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene28922 | -0.820196 | 7.79E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene29773 | 0.80433 | 2.27E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene30246 | 0.819649 | 1.04E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene30855 | 0.818182 | 2.24E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene34233 | 0.839509 | 1.52E-57 | -1.69E-46 |
| MS.gene059569 | MS.gene35909 | 0.813042 | 3.13E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene35910 | 0.81442 | 1.55E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene35967 | 0.837298 | 5.64E-57 | -1.69E-46 |
| MS.gene059569 | MS.gene39955 | 0.817826 | 2.70E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene40811 | 0.801662 | 8.10E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene40837 | -0.800973 | 1.12E-48 | -1.69E-46 |
| MS.gene059569 | MS.gene42580 | 0.817123 | 3.88E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene43491 | 0.805823 | 1.11E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene43992 | 0.802603 | 5.19E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene45340 | 0.821193 | 4.60E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene45567 | 0.807551 | 4.78E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene45578 | 0.823769 | 1.16E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene48059 | 0.808182 | 3.51E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene48435 | 0.800585 | 1.35E-48 | -1.69E-46 |
| MS.gene059569 | MS.gene48490 | 0.807066 | 6.06E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene49161 | 0.804089 | 2.55E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene49193 | 0.807683 | 4.48E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene50033 | 0.804432 | 2.17E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene50990 | 0.80575 | 1.15E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene51106 | -0.81187 | 5.64E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene51441 | 0.851443 | 8.85E-61 | -1.69E-46 |
| MS.gene059569 | MS.gene52066 | 0.820177 | 7.87E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene52448 | 0.80246 | 5.55E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene53579 | 0.826441 | 2.71E-54 | -1.69E-46 |
| MS.gene059569 | MS.gene53586 | 0.831092 | 2.03E-55 | -1.69E-46 |
| MS.gene059569 | MS.gene54473 | 0.815731 | 7.96E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene55988 | 0.814834 | 1.26E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene56468 | 0.818547 | 1.85E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene57160 | -0.801663 | 8.10E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene58503 | 0.804069 | 2.58E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene58928 | -0.812878 | 3.40E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene59876 | 0.8045 | 2.10E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene59949 | 0.810904 | 9.14E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene60206 | 0.811326 | 7.40E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene61139 | 0.805473 | 1.31E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene61183 | 0.802203 | 6.27E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene62393 | 0.811302 | 7.49E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene63893 | 0.802358 | 5.83E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene64243 | 0.832985 | 6.91E-56 | -1.69E-46 |
| MS.gene059569 | MS.gene64249 | 0.826817 | 2.20E-54 | -1.69E-46 |
| MS.gene059569 | MS.gene65221 | 0.801094 | 1.06E-48 | -1.69E-46 |
| MS.gene059569 | MS.gene66474 | 0.804445 | 2.15E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene68873 | 0.818915 | 1.53E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene68933 | 0.807831 | 4.17E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene69477 | 0.814203 | 1.74E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene71949 | 0.822407 | 2.41E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene72099 | 0.804727 | 1.88E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene72128 | 0.806674 | 7.33E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene72130 | 0.812514 | 4.08E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene72268 | 0.811603 | 6.45E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene73820 | 0.803337 | 3.66E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene73877 | 0.826597 | 2.49E-54 | -1.69E-46 |
| MS.gene059569 | MS.gene73879 | 0.827509 | 1.50E-54 | -1.69E-46 |
| MS.gene059569 | MS.gene74444 | 0.82119 | 4.61E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene76035 | 0.801045 | 1.08E-48 | -1.69E-46 |
| MS.gene059569 | MS.gene76118 | -0.813517 | 2.46E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene78478 | 0.80947 | 1.86E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene78659 | 0.811281 | 7.57E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene78682 | 0.800121 | 1.67E-48 | -1.69E-46 |
| MS.gene059569 | MS.gene81375 | 0.823097 | 1.66E-53 | -1.69E-46 |
| MS.gene059569 | MS.gene81653 | 0.800676 | 1.29E-48 | -1.69E-46 |
| MS.gene059569 | MS.gene82879 | 0.805269 | 1.45E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene83091 | 0.814774 | 1.30E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene83120 | 0.812629 | 3.85E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene83547 | 0.806535 | 7.85E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene83734 | 0.80767 | 4.51E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene84086 | 0.811997 | 5.29E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene84865 | 0.803293 | 3.73E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene85663 | 0.824912 | 6.24E-54 | -1.69E-46 |
| MS.gene059569 | MS.gene86353 | 0.838172 | 3.37E-57 | -1.69E-46 |
| MS.gene059569 | MS.gene86380 | 0.809309 | 2.02E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene88024 | -0.829745 | 4.33E-55 | -1.69E-46 |
| MS.gene059569 | MS.gene88025 | -0.826189 | 3.11E-54 | -1.69E-46 |
| MS.gene059569 | MS.gene89511 | 0.816344 | 5.80E-52 | -1.69E-46 |
| MS.gene059569 | MS.gene91907 | 0.805821 | 1.11E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene92548 | 0.801284 | 9.68E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene93052 | 0.80276 | 4.81E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene93135 | 0.805247 | 1.46E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene93202 | 0.811278 | 7.58E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene95039 | 0.80918 | 2.15E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene95486 | 0.834439 | 3.00E-56 | -1.69E-46 |
| MS.gene059569 | MS.gene95625 | 0.810179 | 1.31E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene96220 | 0.81398 | 1.94E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene98141 | 0.803169 | 3.96E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene98277 | 0.80802 | 3.80E-50 | -1.69E-46 |
| MS.gene059569 | MS.gene98543 | 0.812666 | 3.78E-51 | -1.69E-46 |
| MS.gene059569 | MS.gene98633 | 0.802182 | 6.34E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene99313 | 0.827553 | 1.47E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059569.t1 | MTR_6g088605 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 2.84e-78 | 228 |
| MS.gene059569.t1 | MTR_7g103190 | 94.545 | 110 | 6 | 0 | 1 | 110 | 1 | 110 | 1.83e-75 | 220 |
| MS.gene059569.t1 | MTR_5g037160 | 100.000 | 35 | 0 | 0 | 62 | 96 | 409 | 443 | 4.08e-18 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059569.t1 | AT3G18760 | 64.516 | 124 | 40 | 1 | 1 | 124 | 1 | 120 | 2.01e-55 | 169 |
| MS.gene059569.t1 | AT3G18760 | 61.290 | 124 | 39 | 2 | 1 | 124 | 1 | 115 | 1.67e-49 | 154 |
Find 28 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGCTCTTTGTTGATTTC+GGG | 0.181534 | 6.4:+61697332 | None:intergenic |
| ATGCAGCTCTTTGTTGATTT+CGG | 0.208605 | 6.4:+61697331 | None:intergenic |
| GTCACTGAGGTTAAGAGTTT+TGG | 0.239764 | 6.4:-61698788 | MS.gene059569:CDS |
| TCATGCTCACTTGCATCATT+TGG | 0.288871 | 6.4:+61697363 | None:intergenic |
| CTCTAATTGGTTTAGTTTCA+AGG | 0.335670 | 6.4:-61698844 | MS.gene059569:CDS |
| AAAGATTGGGCTTGAGTTCA+TGG | 0.348607 | 6.4:-61697252 | MS.gene059569:CDS |
| GGAACGATTCAGTTGGGTTA+TGG | 0.351815 | 6.4:-61698767 | MS.gene059569:CDS |
| TTGGTTTAGTTTCAAGGATT+GGG | 0.375533 | 6.4:-61698838 | MS.gene059569:CDS |
| ATTGGTTTAGTTTCAAGGAT+TGG | 0.385789 | 6.4:-61698839 | MS.gene059569:CDS |
| GCGTGATGGCAGATTCTATC+AGG | 0.387307 | 6.4:-61698735 | MS.gene059569:intron |
| GTAGTTTGTCCTCCTTGTTT+AGG | 0.396913 | 6.4:+61697306 | None:intergenic |
| ATAAAATCGTTGTGCATCAT+AGG | 0.416306 | 6.4:-61697060 | MS.gene059569:CDS |
| AACCATGTGTGTAAAAGAAA+TGG | 0.437024 | 6.4:-61698815 | MS.gene059569:CDS |
| AAGCAGCGCGACACAAAGAT+TGG | 0.441791 | 6.4:-61697266 | MS.gene059569:CDS |
| ACTATCAATAAGCACATGTT+TGG | 0.443485 | 6.4:+61697035 | None:intergenic |
| ACCATGTGTGTAAAAGAAAT+GGG | 0.451075 | 6.4:-61698814 | MS.gene059569:CDS |
| CAAACTACTGCGTTGGCTAC+TGG | 0.454974 | 6.4:-61697291 | MS.gene059569:CDS |
| AACAAAAGCATGCAATCATA+AGG | 0.460708 | 6.4:+61698890 | None:intergenic |
| CTTGAGTTCATGGGAGATGA+AGG | 0.503780 | 6.4:-61697242 | MS.gene059569:intron |
| AGCAGCGCGACACAAAGATT+GGG | 0.526109 | 6.4:-61697265 | MS.gene059569:CDS |
| AAGATTGGGCTTGAGTTCAT+GGG | 0.546848 | 6.4:-61697251 | MS.gene059569:CDS |
| AGGAGGACAAACTACTGCGT+TGG | 0.582013 | 6.4:-61697298 | MS.gene059569:CDS |
| AAGAAATGGGGTTGTCACTG+AGG | 0.631658 | 6.4:-61698801 | MS.gene059569:CDS |
| AAAAGCATGCAATCATAAGG+TGG | 0.660043 | 6.4:+61698893 | None:intergenic |
| AATGATGCAAGTGAGCATGA+TGG | 0.687340 | 6.4:-61697360 | MS.gene059569:CDS |
| AGAGCTGCATTACCTAAACA+AGG | 0.691182 | 6.4:-61697318 | MS.gene059569:CDS |
| CCATGTGTGTAAAAGAAATG+GGG | 0.732488 | 6.4:-61698813 | MS.gene059569:CDS |
| GCTGCATTACCTAAACAAGG+AGG | 0.778109 | 6.4:-61697315 | MS.gene059569:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGTTTATAAAAATAGTTT+AGG | + | chr6.4:61697969-61697988 | None:intergenic | 10.0% |
| !!! | TATTTTTATAAACTATCTTA+AGG | - | chr6.4:61697973-61697992 | MS.gene059569:intron | 10.0% |
| !! | AAATAAAGTGAACTAAAATA+TGG | - | chr6.4:61698815-61698834 | MS.gene059569:CDS | 15.0% |
| !! | AGTTATTTGATATATTAATG+AGG | - | chr6.4:61697333-61697352 | MS.gene059569:CDS | 15.0% |
| !! | TAAAAAAATAGAATTTGACA+TGG | - | chr6.4:61697908-61697927 | MS.gene059569:intron | 15.0% |
| !! | TTAAATAGATTGTTTGTATT+TGG | - | chr6.4:61697837-61697856 | MS.gene059569:intron | 15.0% |
| !! | TTAATTAAATAGAATACAAG+AGG | - | chr6.4:61697397-61697416 | MS.gene059569:intron | 15.0% |
| !!! | CTTTCTTTTATATATAATCT+AGG | - | chr6.4:61697458-61697477 | MS.gene059569:intron | 15.0% |
| !!! | TTCTATTTTTAATGAATGTA+GGG | - | chr6.4:61698549-61698568 | MS.gene059569:intron | 15.0% |
| !!! | TTTCTATTTTTAATGAATGT+AGG | - | chr6.4:61698548-61698567 | MS.gene059569:intron | 15.0% |
| !! | TAGAGAATTTCAATCAAATA+CGG | + | chr6.4:61697642-61697661 | None:intergenic | 20.0% |
| !! | TCTGTAATAAATAAAGATGA+TGG | - | chr6.4:61698738-61698757 | MS.gene059569:CDS | 20.0% |
| !! | TTGTATTTGGATATAATCAT+TGG | - | chr6.4:61697850-61697869 | MS.gene059569:intron | 20.0% |
| !!! | AATTTTGTAAAACATGTATC+CGG | + | chr6.4:61697252-61697271 | None:intergenic | 20.0% |
| !!! | AGTCTTTTAGATTTATAACA+TGG | + | chr6.4:61698065-61698084 | None:intergenic | 20.0% |
| !!! | TGAATTTTAGAACTTTCAAT+AGG | + | chr6.4:61698352-61698371 | None:intergenic | 20.0% |
| !!! | TGTTTTACAAAATTGACTAT+TGG | - | chr6.4:61697258-61697277 | MS.gene059569:CDS | 20.0% |
| ! | AAATAGAATTTGACATGGAT+AGG | - | chr6.4:61697913-61697932 | MS.gene059569:intron | 25.0% |
| ! | AATAGAATTTGACATGGATA+GGG | - | chr6.4:61697914-61697933 | MS.gene059569:intron | 25.0% |
| ! | CAAATTAATGTGATGCATAT+AGG | - | chr6.4:61697516-61697535 | MS.gene059569:intron | 25.0% |
| ! | CTATATGCATCACATTAATT+TGG | + | chr6.4:61697518-61697537 | None:intergenic | 25.0% |
| ! | TAAGGATGAAATCAATTCAT+GGG | - | chr6.4:61697991-61698010 | MS.gene059569:intron | 25.0% |
| ! | TTAAGGATGAAATCAATTCA+TGG | - | chr6.4:61697990-61698009 | MS.gene059569:intron | 25.0% |
| ! | TTTATTACAGAGAATCTGTT+TGG | + | chr6.4:61698729-61698748 | None:intergenic | 25.0% |
| AACCATGTGTGTAAAAGAAA+TGG | - | chr6.4:61697120-61697139 | MS.gene059569:intron | 30.0% | |
| ACCATGTGTGTAAAAGAAAT+GGG | - | chr6.4:61697121-61697140 | MS.gene059569:intron | 30.0% | |
| AGAATAGTAATATCCACATG+GGG | + | chr6.4:61698109-61698128 | None:intergenic | 30.0% | |
| ATAAAATCGTTGTGCATCAT+AGG | - | chr6.4:61698875-61698894 | MS.gene059569:CDS | 30.0% | |
| ATAGAATTTGACATGGATAG+GGG | - | chr6.4:61697915-61697934 | MS.gene059569:intron | 30.0% | |
| ATATAATCTAGGTGATGTGA+TGG | - | chr6.4:61697469-61697488 | MS.gene059569:intron | 30.0% | |
| GAGAATAGTAATATCCACAT+GGG | + | chr6.4:61698110-61698129 | None:intergenic | 30.0% | |
| TAATAAATCATGCCTGCATT+AGG | + | chr6.4:61697431-61697450 | None:intergenic | 30.0% | |
| TCTACAATATCACAATACCA+AGG | - | chr6.4:61697658-61697677 | MS.gene059569:intron | 30.0% | |
| TGAGAATAGTAATATCCACA+TGG | + | chr6.4:61698111-61698130 | None:intergenic | 30.0% | |
| TTAGTTTAAGAGTCTGTCTT+TGG | - | chr6.4:61698450-61698469 | MS.gene059569:intron | 30.0% | |
| TTGTTAAATAGCAGCTATAG+TGG | - | chr6.4:61698134-61698153 | MS.gene059569:intron | 30.0% | |
| ! | AACAAAAGCATGCAATCATA+AGG | + | chr6.4:61697048-61697067 | None:intergenic | 30.0% |
| ! | AATAAATAAAGATGATGGCG+AGG | - | chr6.4:61698743-61698762 | MS.gene059569:CDS | 30.0% |
| ! | ATTGGTTTAGTTTCAAGGAT+TGG | - | chr6.4:61697096-61697115 | MS.gene059569:intron | 30.0% |
| ! | CTCTAATTGGTTTAGTTTCA+AGG | - | chr6.4:61697091-61697110 | MS.gene059569:intron | 30.0% |
| ! | GTGAAAAAAGAAGCTCTAAT+TGG | - | chr6.4:61697078-61697097 | MS.gene059569:intron | 30.0% |
| ! | TTGGTTTAGTTTCAAGGATT+GGG | - | chr6.4:61697097-61697116 | MS.gene059569:intron | 30.0% |
| !!! | AGAGCTTCTTTTTTCACATT+CGG | + | chr6.4:61697075-61697094 | None:intergenic | 30.0% |
| !!! | ATAGGCAGGGTTTTAAAAAA+TGG | - | chr6.4:61697534-61697553 | MS.gene059569:intron | 30.0% |
| !!! | CTGTTTTGTTTGTTTCCATT+AGG | - | chr6.4:61698847-61698866 | MS.gene059569:CDS | 30.0% |
| AATAGCAGCTATAGTGGTAT+TGG | - | chr6.4:61698140-61698159 | MS.gene059569:intron | 35.0% | |
| ACATGACATAACTCTTGGTT+TGG | - | chr6.4:61697784-61697803 | MS.gene059569:intron | 35.0% | |
| CATGACATAACTCTTGGTTT+GGG | - | chr6.4:61697785-61697804 | MS.gene059569:intron | 35.0% | |
| CATGGCATAAGTACTTGTTT+AGG | + | chr6.4:61698047-61698066 | None:intergenic | 35.0% | |
| CCATGTGTGTAAAAGAAATG+GGG | - | chr6.4:61697122-61697141 | MS.gene059569:intron | 35.0% | |
| GTACTTGTTTAGGTAGTGTT+TGG | + | chr6.4:61698037-61698056 | None:intergenic | 35.0% | |
| GTTTCACATGACATAACTCT+TGG | - | chr6.4:61697779-61697798 | MS.gene059569:intron | 35.0% | |
| TAATGTGATGCATATAGGCA+GGG | - | chr6.4:61697521-61697540 | MS.gene059569:intron | 35.0% | |
| TATATTAATGAGGTGCGTCA+AGG | - | chr6.4:61697343-61697362 | MS.gene059569:CDS | 35.0% | |
| TCATAGATCGTGGAAAATAG+CGG | + | chr6.4:61698275-61698294 | None:intergenic | 35.0% | |
| TCTGTTGTAATCATAGATCG+TGG | + | chr6.4:61698285-61698304 | None:intergenic | 35.0% | |
| TTAATGTGATGCATATAGGC+AGG | - | chr6.4:61697520-61697539 | MS.gene059569:intron | 35.0% | |
| TTGTTTGTTTCCATTAGGAG+AGG | - | chr6.4:61698852-61698871 | MS.gene059569:CDS | 35.0% | |
| ! | AAAAGCATGCAATCATAAGG+TGG | + | chr6.4:61697045-61697064 | None:intergenic | 35.0% |
| ! | ACGATTTTATCCTCTCCTAA+TGG | + | chr6.4:61698865-61698884 | None:intergenic | 35.0% |
| ! | AGAGTTTAAAACAGAGGTCA+GGG | + | chr6.4:61697708-61697727 | None:intergenic | 35.0% |
| ! | ATGACATAACTCTTGGTTTG+GGG | - | chr6.4:61697786-61697805 | MS.gene059569:intron | 35.0% |
| ! | ATGCACAGAGTTTAAAACAG+AGG | + | chr6.4:61697714-61697733 | None:intergenic | 35.0% |
| ! | CTCACACTCATTATTTTCAC+CGG | - | chr6.4:61697230-61697249 | MS.gene059569:intron | 35.0% |
| !! | ATGCAGCTCTTTGTTGATTT+CGG | + | chr6.4:61698607-61698626 | None:intergenic | 35.0% |
| !! | CTGTTTTAAACTCTGTGCAT+CGG | - | chr6.4:61697714-61697733 | MS.gene059569:intron | 35.0% |
| !!! | AGTTTTGGAACGATTCAGTT+GGG | - | chr6.4:61697162-61697181 | MS.gene059569:intron | 35.0% |
| AAAAGAGTTGCACGTTCTTC+TGG | - | chr6.4:61698480-61698499 | MS.gene059569:intron | 40.0% | |
| AAAGATTGGGCTTGAGTTCA+TGG | - | chr6.4:61698683-61698702 | MS.gene059569:intron | 40.0% | |
| AAATCATGCCTGCATTAGGT+TGG | + | chr6.4:61697427-61697446 | None:intergenic | 40.0% | |
| AACGACAACGTGATTGTGAT+TGG | - | chr6.4:61697605-61697624 | MS.gene059569:intron | 40.0% | |
| AAGATTGGGCTTGAGTTCAT+GGG | - | chr6.4:61698684-61698703 | MS.gene059569:intron | 40.0% | |
| AATGATGCAAGTGAGCATGA+TGG | - | chr6.4:61698575-61698594 | MS.gene059569:intron | 40.0% | |
| ACGACAACGTGATTGTGATT+GGG | - | chr6.4:61697606-61697625 | MS.gene059569:intron | 40.0% | |
| AGAGCTGCATTACCTAAACA+AGG | - | chr6.4:61698617-61698636 | MS.gene059569:intron | 40.0% | |
| TCATGCTCACTTGCATCATT+TGG | + | chr6.4:61698575-61698594 | None:intergenic | 40.0% | |
| ! | ATGGTTTGAAACTGTGCTAC+AGG | - | chr6.4:61697553-61697572 | MS.gene059569:intron | 40.0% |
| ! | CAGAGTTTAAAACAGAGGTC+AGG | + | chr6.4:61697709-61697728 | None:intergenic | 40.0% |
| ! | CCCCATTTCTTTTACACACA+TGG | + | chr6.4:61697125-61697144 | None:intergenic | 40.0% |
| ! | GTAGTTTGTCCTCCTTGTTT+AGG | + | chr6.4:61698632-61698651 | None:intergenic | 40.0% |
| ! | GTCACTGAGGTTAAGAGTTT+TGG | - | chr6.4:61697147-61697166 | MS.gene059569:intron | 40.0% |
| ! | TATGGTGTGAAAAAGCGTGA+TGG | - | chr6.4:61697186-61697205 | MS.gene059569:intron | 40.0% |
| ! | TCACAATCACGTTGTCGTTT+CGG | + | chr6.4:61697604-61697623 | None:intergenic | 40.0% |
| ! | TGGTTTGAAACTGTGCTACA+GGG | - | chr6.4:61697554-61697573 | MS.gene059569:intron | 40.0% |
| !! | TGACATAACTCTTGGTTTGG+GGG | - | chr6.4:61697787-61697806 | MS.gene059569:intron | 40.0% |
| !! | TGCAGCTCTTTGTTGATTTC+GGG | + | chr6.4:61698606-61698625 | None:intergenic | 40.0% |
| !!! | GAGTTTTGGAACGATTCAGT+TGG | - | chr6.4:61697161-61697180 | MS.gene059569:intron | 40.0% |
| AAGAAATGGGGTTGTCACTG+AGG | - | chr6.4:61697134-61697153 | MS.gene059569:intron | 45.0% | |
| CATGGATAGGGGACAAAAAG+TGG | - | chr6.4:61697926-61697945 | MS.gene059569:intron | 45.0% | |
| CGACAACGTGATTGTGATTG+GGG | - | chr6.4:61697607-61697626 | MS.gene059569:intron | 45.0% | |
| CGGTATGTTCAAACTCCACT+AGG | + | chr6.4:61698255-61698274 | None:intergenic | 45.0% | |
| CTTGAGTTCATGGGAGATGA+AGG | - | chr6.4:61698693-61698712 | MS.gene059569:intron | 45.0% | |
| GCTGCATTACCTAAACAAGG+AGG | - | chr6.4:61698620-61698639 | MS.gene059569:intron | 45.0% | |
| GGAACGATTCAGTTGGGTTA+TGG | - | chr6.4:61697168-61697187 | MS.gene059569:intron | 45.0% | |
| !! | ATAACTCTTGGTTTGGGGGT+AGG | - | chr6.4:61697791-61697810 | MS.gene059569:intron | 45.0% |
| !! | CGTTCTTCTGGAACTTCGAA+TGG | - | chr6.4:61698492-61698511 | MS.gene059569:intron | 45.0% |
| AAGCAGCGCGACACAAAGAT+TGG | - | chr6.4:61698669-61698688 | MS.gene059569:intron | 50.0% | |
| AGAGGTCAGGGTAACTACTG+TGG | + | chr6.4:61697696-61697715 | None:intergenic | 50.0% | |
| AGCAGCGCGACACAAAGATT+GGG | - | chr6.4:61698670-61698689 | MS.gene059569:intron | 50.0% | |
| AGGAGGACAAACTACTGCGT+TGG | - | chr6.4:61698637-61698656 | MS.gene059569:intron | 50.0% | |
| CCTCTTCTAAATGCCCCATG+TGG | - | chr6.4:61698093-61698112 | MS.gene059569:intron | 50.0% | |
| GCGTGATGGCAGATTCTATC+AGG | - | chr6.4:61697200-61697219 | MS.gene059569:intron | 50.0% | |
| GCTATAGCACTGTTGCCTAG+TGG | - | chr6.4:61698237-61698256 | MS.gene059569:intron | 50.0% | |
| GGCGAGGATAGTGATACAGA+CGG | - | chr6.4:61698759-61698778 | MS.gene059569:CDS | 50.0% | |
| ! | CCACATGGGGCATTTAGAAG+AGG | + | chr6.4:61698096-61698115 | None:intergenic | 50.0% |
| ! | GAGGTACTCCAACCTAATGC+AGG | - | chr6.4:61697416-61697435 | MS.gene059569:intron | 50.0% |
| ! | GTGCATCGGTAGATGCGAAA+TGG | - | chr6.4:61697728-61697747 | MS.gene059569:intron | 50.0% |
| !! | CAAACTACTGCGTTGGCTAC+TGG | - | chr6.4:61698644-61698663 | MS.gene059569:intron | 50.0% |
| !! | GGGGGTAGGAGTTGATTTGT+TGG | - | chr6.4:61697805-61697824 | MS.gene059569:intron | 50.0% |
| TGTGGTCGCTTCACGATCCT+TGG | + | chr6.4:61697678-61697697 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 61697039 | 61698918 | 61697039 | ID=MS.gene059569 |
| chr6.4 | mRNA | 61697039 | 61698918 | 61697039 | ID=MS.gene059569.t1;Parent=MS.gene059569 |
| chr6.4 | exon | 61698736 | 61698918 | 61698736 | ID=MS.gene059569.t1.exon1;Parent=MS.gene059569.t1 |
| chr6.4 | CDS | 61698736 | 61698918 | 61698736 | ID=cds.MS.gene059569.t1;Parent=MS.gene059569.t1 |
| chr6.4 | exon | 61697243 | 61697387 | 61697243 | ID=MS.gene059569.t1.exon2;Parent=MS.gene059569.t1 |
| chr6.4 | CDS | 61697243 | 61697387 | 61697243 | ID=cds.MS.gene059569.t1;Parent=MS.gene059569.t1 |
| chr6.4 | exon | 61697039 | 61697088 | 61697039 | ID=MS.gene059569.t1.exon3;Parent=MS.gene059569.t1 |
| chr6.4 | CDS | 61697039 | 61697088 | 61697039 | ID=cds.MS.gene059569.t1;Parent=MS.gene059569.t1 |
| Gene Sequence |
| Protein sequence |
