Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89501.t1 | XP_003615024.1 | 99.5 | 386 | 2 | 0 | 2 | 387 | 1 | 386 | 9.20E-224 | 785.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89501.t1 | Q803I2 | 52.7 | 393 | 162 | 4 | 6 | 387 | 4 | 383 | 1.8e-119 | 430.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89501.t1 | G7KA80 | 99.5 | 386 | 2 | 0 | 2 | 387 | 1 | 386 | 6.6e-224 | 785.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene89501 | 0.804878 | 1.75E-49 | -1.69E-46 |
| MS.gene060929 | MS.gene89501 | 0.82355 | 1.30E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044295 | MS.gene89501 | PPI |
| MS.gene89501 | MS.gene70505 | PPI |
| MS.gene89501 | MS.gene25743 | PPI |
| MS.gene69767 | MS.gene89501 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89501.t1 | MTR_5g062720 | 99.482 | 386 | 2 | 0 | 2 | 387 | 1 | 386 | 0.0 | 802 |
| MS.gene89501.t1 | MTR_5g062720 | 99.340 | 303 | 2 | 0 | 2 | 304 | 1 | 303 | 0.0 | 634 |
| MS.gene89501.t1 | MTR_4g094255 | 68.312 | 385 | 120 | 1 | 2 | 386 | 1 | 383 | 0.0 | 593 |
| MS.gene89501.t1 | MTR_4g094255 | 66.554 | 296 | 97 | 1 | 91 | 386 | 1 | 294 | 7.37e-159 | 448 |
| MS.gene89501.t1 | MTR_3g081480 | 36.148 | 379 | 199 | 5 | 9 | 379 | 7 | 350 | 2.23e-76 | 240 |
| MS.gene89501.t1 | MTR_4g080800 | 32.683 | 205 | 97 | 9 | 203 | 385 | 290 | 475 | 1.90e-19 | 90.1 |
| MS.gene89501.t1 | MTR_4g080800 | 38.318 | 107 | 65 | 1 | 7 | 112 | 5 | 111 | 1.10e-15 | 79.0 |
| MS.gene89501.t1 | MTR_2g083260 | 28.470 | 281 | 159 | 10 | 125 | 385 | 220 | 478 | 1.39e-18 | 87.4 |
| MS.gene89501.t1 | MTR_2g083260 | 37.273 | 110 | 68 | 1 | 4 | 112 | 2 | 111 | 3.07e-15 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89501.t1 | AT1G36050 | 81.865 | 386 | 70 | 0 | 2 | 387 | 1 | 386 | 0.0 | 697 |
| MS.gene89501.t1 | AT1G36050 | 81.675 | 382 | 70 | 0 | 2 | 383 | 1 | 382 | 0.0 | 689 |
| MS.gene89501.t1 | AT1G22200 | 77.979 | 386 | 85 | 0 | 2 | 387 | 1 | 386 | 0.0 | 656 |
| MS.gene89501.t1 | AT1G22200 | 77.015 | 335 | 77 | 0 | 2 | 336 | 1 | 335 | 0.0 | 565 |
| MS.gene89501.t1 | AT3G22290 | 36.031 | 383 | 202 | 10 | 5 | 380 | 3 | 349 | 4.28e-75 | 237 |
| MS.gene89501.t1 | AT3G22290 | 36.031 | 383 | 202 | 10 | 5 | 380 | 3 | 349 | 4.28e-75 | 237 |
| MS.gene89501.t1 | AT4G27080 | 31.841 | 201 | 104 | 7 | 203 | 385 | 293 | 478 | 2.00e-21 | 96.3 |
| MS.gene89501.t1 | AT4G27080 | 37.963 | 108 | 66 | 1 | 7 | 113 | 5 | 112 | 5.71e-16 | 79.7 |
| MS.gene89501.t1 | AT4G27080 | 31.841 | 201 | 104 | 7 | 203 | 385 | 345 | 530 | 3.42e-21 | 95.5 |
| MS.gene89501.t1 | AT4G27080 | 38.614 | 101 | 61 | 1 | 14 | 113 | 64 | 164 | 1.06e-13 | 72.8 |
| MS.gene89501.t1 | AT3G20560 | 30.693 | 202 | 108 | 7 | 203 | 385 | 293 | 481 | 5.23e-18 | 85.9 |
| MS.gene89501.t1 | AT3G20560 | 40.187 | 107 | 63 | 1 | 8 | 113 | 6 | 112 | 1.96e-17 | 84.3 |
| MS.gene89501.t1 | AT1G50950 | 26.690 | 281 | 167 | 9 | 125 | 385 | 226 | 487 | 6.10e-17 | 82.8 |
| MS.gene89501.t1 | AT1G50950 | 39.815 | 108 | 64 | 1 | 7 | 113 | 5 | 112 | 1.41e-16 | 81.6 |
| MS.gene89501.t1 | AT1G50950 | 26.690 | 281 | 167 | 9 | 125 | 385 | 221 | 482 | 7.02e-17 | 82.4 |
| MS.gene89501.t1 | AT1G50950 | 39.815 | 108 | 64 | 1 | 7 | 113 | 5 | 112 | 1.42e-16 | 81.6 |
Find 81 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTATAGTCGCACTCTTTC+TGG | 0.133582 | 5.4:-49011020 | MS.gene89501:CDS |
| CTACGACCTTTCTCCAATTA+AGG | 0.195998 | 5.4:-49000563 | MS.gene89501:intron |
| ATTACGAAGCTTGTTCATTA+TGG | 0.253518 | 5.4:+49011073 | None:intergenic |
| TCTTCTTCTTGATAGCCTTT+TGG | 0.307819 | 5.4:+48999551 | None:intergenic |
| GGCCGATTGCAGTCCCTCCC+TGG | 0.324323 | 5.4:-49000598 | MS.gene89501:CDS |
| CATTTGTATACCTCCAACTA+TGG | 0.339863 | 5.4:+48999737 | None:intergenic |
| TCTCTGTGACAGAACACTTT+AGG | 0.345410 | 5.4:-49000633 | MS.gene89501:CDS |
| TACCGCCTCAGCACCATAAC+AGG | 0.379587 | 5.4:+49008958 | None:intergenic |
| CTAGATTCATTTATCTATCA+TGG | 0.385708 | 5.4:-48999574 | MS.gene89501:CDS |
| GCCGATTGCAGTCCCTCCCT+GGG | 0.388413 | 5.4:-49000597 | MS.gene89501:CDS |
| GGGAAAAGCTTTCAGCAATC+TGG | 0.414672 | 5.4:-49005959 | MS.gene89501:CDS |
| CCCCAGGGAGGGACTGCAAT+CGG | 0.422047 | 5.4:+49000596 | None:intergenic |
| CAAACCTGGTCAATTGAATC+CGG | 0.433588 | 5.4:+49006926 | None:intergenic |
| GATCAATAACCTGGTTTGAC+TGG | 0.437886 | 5.4:+49000731 | None:intergenic |
| ACTTATTGCGGATCCTGTTA+TGG | 0.450203 | 5.4:-49008971 | MS.gene89501:CDS |
| TTGGAAGTAAACAAGGTGGC+AGG | 0.459413 | 5.4:-49006001 | MS.gene89501:CDS |
| CGTACATGTGCATGACCTGC+TGG | 0.468545 | 5.4:-49005936 | MS.gene89501:CDS |
| CTGCCTCCATGTCTTTGCAA+GGG | 0.472630 | 5.4:+49009010 | None:intergenic |
| GAAAAGCAAGAGCATGAGAA+TGG | 0.476070 | 5.4:+49010974 | None:intergenic |
| CACATCAACAGAATAGCCTT+TGG | 0.482453 | 5.4:-49003539 | MS.gene89501:CDS |
| CCTGCCTCCATGTCTTTGCA+AGG | 0.483999 | 5.4:+49009009 | None:intergenic |
| CATTAGCGGAGAACAACATC+TGG | 0.491394 | 5.4:-49009624 | MS.gene89501:CDS |
| GAAAGAGAAATTACGAAGCT+CGG | 0.495749 | 5.4:+49010947 | None:intergenic |
| GAAAGAATCAAGGAAGAAGA+AGG | 0.501222 | 5.4:-49006049 | MS.gene89501:CDS |
| AAGCATATCGAAAGAAAGGT+TGG | 0.504184 | 5.4:-49006961 | MS.gene89501:CDS |
| CGTGAAGCATATCGAAAGAA+AGG | 0.504663 | 5.4:-49006965 | MS.gene89501:CDS |
| GAAACAAGGCAAGATGGAAT+CGG | 0.513498 | 5.4:-49009434 | MS.gene89501:CDS |
| ATTTATCTATCATGGCCAAA+AGG | 0.514131 | 5.4:-48999566 | MS.gene89501:CDS |
| GTGATAGAAACAAGGCAAGA+TGG | 0.530527 | 5.4:-49009440 | MS.gene89501:CDS |
| ACCAATGTGTGTGCCATAGT+TGG | 0.531594 | 5.4:-48999750 | MS.gene89501:CDS |
| TCTTGATTATCAGTGTGCAT+TGG | 0.532026 | 5.4:-49003395 | MS.gene89501:intron |
| AAAACACCTTAATTGGAGAA+AGG | 0.535317 | 5.4:+49000557 | None:intergenic |
| AAAGGCTATCAAGAAGAAGA+TGG | 0.538983 | 5.4:-48999548 | MS.gene89501:CDS |
| TTGTTCTCCGCTAATGTCCA+TGG | 0.540332 | 5.4:+49009631 | None:intergenic |
| CTCGATGCCATGGACATTAG+CGG | 0.540952 | 5.4:-49009638 | MS.gene89501:CDS |
| GTCAAGAGGGTTTACAACAC+CGG | 0.543744 | 5.4:+49003505 | None:intergenic |
| CTCACGTCAGTGTACATAGT+TGG | 0.544532 | 5.4:+49000766 | None:intergenic |
| ATCACTTAGTGCAAAAGAGA+AGG | 0.545278 | 5.4:-49006079 | MS.gene89501:intron |
| TCCAACTATGGCACACACAT+TGG | 0.549757 | 5.4:+48999749 | None:intergenic |
| AGGGTTCTTAGAAAGAATCA+AGG | 0.554292 | 5.4:-49006059 | MS.gene89501:CDS |
| AAAATACTGATACATCCCAC+TGG | 0.555074 | 5.4:+49003359 | None:intergenic |
| AGCATATCGAAAGAAAGGTT+GGG | 0.557532 | 5.4:-49006960 | MS.gene89501:CDS |
| CCTTGCAAAGACATGGAGGC+AGG | 0.557759 | 5.4:-49009009 | MS.gene89501:CDS |
| AGGCTATTCTGTTGATGTGG+TGG | 0.558845 | 5.4:+49003543 | None:intergenic |
| AGTATAGAACACGCTAGTGC+AGG | 0.559960 | 5.4:+49009665 | None:intergenic |
| TATACTTAGTCTCGATGCCA+TGG | 0.561660 | 5.4:-49009648 | MS.gene89501:CDS |
| GGTGAAGGATGCAATGTGTA+TGG | 0.569301 | 5.4:-49006028 | MS.gene89501:CDS |
| AAGAAAAGAATAGACTCTCA+TGG | 0.572185 | 5.4:-49009467 | MS.gene89501:CDS |
| GTGTTGTAAACCCTCTTGAC+CGG | 0.574958 | 5.4:-49003502 | MS.gene89501:intron |
| AGGTTGGGCACTCTCAAGCC+CGG | 0.575134 | 5.4:-49006945 | MS.gene89501:CDS |
| AAACCTGGTCAATTGAATCC+GGG | 0.575289 | 5.4:+49006927 | None:intergenic |
| AAGCCCGGATTCAATTGACC+AGG | 0.575812 | 5.4:-49006930 | MS.gene89501:intron |
| ATCAAGGAAGAAGAAGGTGA+AGG | 0.577027 | 5.4:-49006043 | MS.gene89501:CDS |
| CAAAGGCTATTCTGTTGATG+TGG | 0.582521 | 5.4:+49003540 | None:intergenic |
| TAAAGAGCTCACCGGTCAAG+AGG | 0.585229 | 5.4:+49003491 | None:intergenic |
| TCAAGAGGGTTTACAACACC+GGG | 0.589163 | 5.4:+49003506 | None:intergenic |
| TGCTTCACGAACTTCCTCAC+AGG | 0.590612 | 5.4:+49006979 | None:intergenic |
| TCACTTAGTGCAAAAGAGAA+GGG | 0.593153 | 5.4:-49006078 | MS.gene89501:intron |
| TGGAAGTAAACAAGGTGGCA+GGG | 0.593479 | 5.4:-49006000 | MS.gene89501:CDS |
| ATCAAGAAGAAGATGGAACT+CGG | 0.594892 | 5.4:-48999541 | MS.gene89501:CDS |
| TTTGCGCTTGCAGTCAGATG+AGG | 0.596325 | 5.4:-49007014 | MS.gene89501:intron |
| CACTTTAGGACGGCAGATGT+AGG | 0.596476 | 5.4:-49000619 | MS.gene89501:CDS |
| CTTGTTGTAGATACTTCTAG+AGG | 0.598128 | 5.4:-49010819 | MS.gene89501:CDS |
| GAGCACAATGAGACTTATTG+CGG | 0.606344 | 5.4:-49008983 | MS.gene89501:CDS |
| CTATGTACACTGACGTGAGT+GGG | 0.606656 | 5.4:-49000762 | MS.gene89501:CDS |
| AAAGAGCTCACCGGTCAAGA+GGG | 0.621153 | 5.4:+49003492 | None:intergenic |
| ATCCTGTTATGGTGCTGAGG+CGG | 0.626803 | 5.4:-49008960 | MS.gene89501:intron |
| TATAGTCGCACTCTTTCTGG+CGG | 0.635746 | 5.4:-49011017 | MS.gene89501:CDS |
| GAACACTATCCAGTCAAACC+AGG | 0.636074 | 5.4:-49000740 | MS.gene89501:intron |
| ACCGGGAAAATAGTCTCCAA+AGG | 0.641478 | 5.4:+49003523 | None:intergenic |
| CGGATCCTGTTATGGTGCTG+AGG | 0.643851 | 5.4:-49008963 | MS.gene89501:CDS |
| AAATACTGATACATCCCACT+GGG | 0.661329 | 5.4:+49003360 | None:intergenic |
| TGTGACAGAACACTTTAGGA+CGG | 0.663702 | 5.4:-49000629 | MS.gene89501:CDS |
| ACTATGTACACTGACGTGAG+TGG | 0.665706 | 5.4:-49000763 | MS.gene89501:CDS |
| CCGATTGCAGTCCCTCCCTG+GGG | 0.667693 | 5.4:-49000596 | MS.gene89501:CDS |
| AATGTGTGTGCCATAGTTGG+AGG | 0.674458 | 5.4:-48999747 | MS.gene89501:intron |
| ATGGCAATGTGATAGAAACA+AGG | 0.687509 | 5.4:-49009448 | MS.gene89501:CDS |
| AAACCCTTGCAAAGACATGG+AGG | 0.692409 | 5.4:-49009013 | MS.gene89501:CDS |
| GGACACAAGAAACACCCAGT+GGG | 0.693082 | 5.4:-49003374 | MS.gene89501:CDS |
| TGGACACAAGAAACACCCAG+TGG | 0.786125 | 5.4:-49003375 | MS.gene89501:CDS |
| GGAATGTTGTAATTCCTGTG+AGG | 0.801380 | 5.4:-49006993 | MS.gene89501:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 48999528 | 49011105 | 48999528 | ID=MS.gene89501 |
| chr5.4 | mRNA | 48999528 | 49011105 | 48999528 | ID=MS.gene89501.t1;Parent=MS.gene89501 |
| chr5.4 | exon | 49010961 | 49011105 | 49010961 | ID=MS.gene89501.t1.exon1;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 49010961 | 49011105 | 49010961 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 49010800 | 49010873 | 49010800 | ID=MS.gene89501.t1.exon2;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 49010800 | 49010873 | 49010800 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 49009619 | 49009702 | 49009619 | ID=MS.gene89501.t1.exon3;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 49009619 | 49009702 | 49009619 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 49009424 | 49009504 | 49009424 | ID=MS.gene89501.t1.exon4;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 49009424 | 49009504 | 49009424 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 49008961 | 49009041 | 49008961 | ID=MS.gene89501.t1.exon5;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 49008961 | 49009041 | 49008961 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 49006931 | 49007023 | 49006931 | ID=MS.gene89501.t1.exon6;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 49006931 | 49007023 | 49006931 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 49005913 | 49006092 | 49005913 | ID=MS.gene89501.t1.exon7;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 49005913 | 49006092 | 49005913 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 49003503 | 49003570 | 49003503 | ID=MS.gene89501.t1.exon8;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 49003503 | 49003570 | 49003503 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 49003353 | 49003404 | 49003353 | ID=MS.gene89501.t1.exon9;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 49003353 | 49003404 | 49003353 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 49000741 | 49000794 | 49000741 | ID=MS.gene89501.t1.exon10;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 49000741 | 49000794 | 49000741 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 49000564 | 49000656 | 49000564 | ID=MS.gene89501.t1.exon11;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 49000564 | 49000656 | 49000564 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 48999748 | 48999814 | 48999748 | ID=MS.gene89501.t1.exon12;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 48999748 | 48999814 | 48999748 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| chr5.4 | exon | 48999528 | 48999619 | 48999528 | ID=MS.gene89501.t1.exon13;Parent=MS.gene89501.t1 |
| chr5.4 | CDS | 48999528 | 48999619 | 48999528 | ID=cds.MS.gene89501.t1;Parent=MS.gene89501.t1 |
| Gene Sequence |
| Protein sequence |
