AlfalfaGEDB | MS.gene90209

M. sativa cultivar XinJiangDaYe / MS.gene90209

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90209.t1	XP_003608424.1	97.1	35	1	0	1	35	1	35	3.00E-06	61.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90209.t1	G7JV73	97.1	35	1	0	1	35	1	35	2.2e-06	61.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050082	MS.gene90209	0.887316	1.59E-72	-1.69E-46
MS.gene051422	MS.gene90209	0.807154	5.81E-50	-1.69E-46
MS.gene053568	MS.gene90209	0.989021	2.29E-176	-1.69E-46
MS.gene053569	MS.gene90209	0.937304	4.31E-98	-1.69E-46
MS.gene057717	MS.gene90209	0.826227	3.05E-54	-1.69E-46
MS.gene058067	MS.gene90209	0.803943	2.74E-49	-1.69E-46
MS.gene058068	MS.gene90209	0.850852	1.30E-60	-1.69E-46
MS.gene061044	MS.gene90209	0.813492	2.49E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90209.t1	MTR_4g093940	93.750	128	3	1	1	128	1	123	1.89e-67	199
MS.gene90209.t1	MTR_4g093950	93.750	112	7	0	1	112	1	112	1.12e-49	155
MS.gene90209.t1	MTR_4g093910	92.857	112	8	0	1	112	1	112	1.10e-48	152
MS.gene90209.t1	MTR_4g093920	91.743	109	8	1	1	109	1	108	2.19e-47	149
MS.gene90209.t1	MTR_5g064530	65.132	152	27	4	1	128	1	150	8.34e-42	135
MS.gene90209.t1	MTR_5g064510	66.667	123	21	4	1	105	1	121	4.06e-36	121
MS.gene90209.t1	MTR_4g094678	69.620	79	6	3	17	92	1	64	1.75e-22	84.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 44 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATTTCCTTGAGGCATATT+TGG	0.193132	4.2:+19776022	None:intergenic
GTGGGAATGGTAGGTAAATT+TGG	0.271860	4.2:+19776085	None:intergenic
GCAATAGATGGCGAGCTTCT+AGG	0.294792	4.2:+19776122	None:intergenic
GGTGGTAATGTAGTTGGTTT+TGG	0.298989	4.2:+19776058	None:intergenic
TAAAGCTGCAATGAAGGATT+TGG	0.355352	4.2:+19776174	None:intergenic
AGATGGAATTGTTGGAATAT+TGG	0.374933	4.2:+19775934	None:intergenic
GTGTTGTTGTTTGCAATAGA+TGG	0.376065	4.2:+19776110	None:intergenic
GGGAAAGTGGTTGGGATTGA+AGG	0.382492	4.2:+19775866	None:intergenic
GGCTAGAAGTTGAAGGTGGT+GGG	0.405223	4.2:+19775825	None:intergenic
TGAGGCATATTTGGAATTGA+AGG	0.405314	4.2:+19776031	None:intergenic
ATAGTGAGTTTAGGCATAGA+AGG	0.436994	4.2:+19775980	None:intergenic
GGGAAGGAGGGGAAAGTGGT+TGG	0.439412	4.2:+19775857	None:intergenic
GGAAGGAGGGGAAAGTGGTT+GGG	0.444157	4.2:+19775858	None:intergenic
GGCAAAGGTGGTAATGTAGT+TGG	0.444412	4.2:+19776052	None:intergenic
GGTTGGGATTGAAGGAAGGT+TGG	0.454159	4.2:+19775874	None:intergenic
AGTGGTGGCATAGTGAGTTT+AGG	0.457061	4.2:+19775971	None:intergenic
AAATGGGAAGGAGGGGAAAG+TGG	0.467197	4.2:+19775853	None:intergenic
GTTGGGATTGAAGGAAGGTT+GGG	0.469467	4.2:+19775875	None:intergenic
TTAGGCATAGAAGGGATAGT+AGG	0.471087	4.2:+19775989	None:intergenic
GGGCTAGAAGTTGAAGGTGG+TGG	0.472490	4.2:+19775824	None:intergenic
AAGAAGTAAAGCTGCAATGA+AGG	0.485067	4.2:+19776168	None:intergenic
GTTTAAGGGCTAGAAGTTGA+AGG	0.490262	4.2:+19775818	None:intergenic
GGAATATTGGTAGGAAGACT+TGG	0.503031	4.2:+19775947	None:intergenic
AAGTGGTTGGGATTGAAGGA+AGG	0.508086	4.2:+19775870	None:intergenic
TGCAATGAAGGATTTGGTTG+AGG	0.510590	4.2:+19776180	None:intergenic
TTTGGTAAAGTGGGAATGGT+AGG	0.519398	4.2:+19776076	None:intergenic
AGTGAAGTGGCTGCTGGCAA+TGG	0.520284	4.2:+19775899	None:intergenic
GGAATTGTTGGAATATTGGT+AGG	0.523994	4.2:+19775938	None:intergenic
ATGTTGAGAGATGGAATTGT+TGG	0.529497	4.2:+19775926	None:intergenic
TTGGTAGGAAGACTTGGAAG+TGG	0.530626	4.2:+19775953	None:intergenic
GAAGGGATAGTAGGCAATGG+AGG	0.560608	4.2:+19775998	None:intergenic
GAAGTGGCTGCTGGCAATGG+TGG	0.580562	4.2:+19775902	None:intergenic
TTTGGAATTGAAGGCAAAGG+TGG	0.580888	4.2:+19776040	None:intergenic
TTGGGAAGTGAAGTGGCTGC+TGG	0.584437	4.2:+19775893	None:intergenic
TCAATTCCAAATATGCCTCA+AGG	0.589431	4.2:-19776028	MS.gene90209:CDS
GCTAGAAGTTGAAGGTGGTG+GGG	0.609650	4.2:+19775826	None:intergenic
GTAGGAAGACTTGGAAGTGG+TGG	0.611131	4.2:+19775956	None:intergenic
TAAGGGCTAGAAGTTGAAGG+TGG	0.613687	4.2:+19775821	None:intergenic
ATATTTGGAATTGAAGGCAA+AGG	0.615183	4.2:+19776037	None:intergenic
TAGTGAGTTTAGGCATAGAA+GGG	0.616158	4.2:+19775981	None:intergenic
ATAGAAGGGATAGTAGGCAA+TGG	0.624968	4.2:+19775995	None:intergenic
AGGAAGGTTGGGAAGTGAAG+TGG	0.630070	4.2:+19775886	None:intergenic
AATGGTGGAATGTTGAGAGA+TGG	0.653205	4.2:+19775917	None:intergenic
AATGGAGGAAGATTTCCTTG+AGG	0.717776	4.2:+19776013	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGATGGAATTGTTGGAATAT+TGG	+	chr4.2:19776073-19776092	None:intergenic	30.0%
!	ATATTTGGAATTGAAGGCAA+AGG	+	chr4.2:19775970-19775989	None:intergenic	30.0%
	AAGAAGTAAAGCTGCAATGA+AGG	+	chr4.2:19775839-19775858	None:intergenic	35.0%
	AGATTTCCTTGAGGCATATT+TGG	+	chr4.2:19775985-19776004	None:intergenic	35.0%
	ATAGTGAGTTTAGGCATAGA+AGG	+	chr4.2:19776027-19776046	None:intergenic	35.0%
	ATGTTGAGAGATGGAATTGT+TGG	+	chr4.2:19776081-19776100	None:intergenic	35.0%
	GGAATTGTTGGAATATTGGT+AGG	+	chr4.2:19776069-19776088	None:intergenic	35.0%
	TAAAGCTGCAATGAAGGATT+TGG	+	chr4.2:19775833-19775852	None:intergenic	35.0%
	TAGTGAGTTTAGGCATAGAA+GGG	+	chr4.2:19776026-19776045	None:intergenic	35.0%
	TCAATTCCAAATATGCCTCA+AGG	-	chr4.2:19775976-19775995	MS.gene90209:CDS	35.0%
!	TGAGGCATATTTGGAATTGA+AGG	+	chr4.2:19775976-19775995	None:intergenic	35.0%
!!	GTGTTGTTGTTTGCAATAGA+TGG	+	chr4.2:19775897-19775916	None:intergenic	35.0%
!!!	GTAGTTGGTTTTGGTAAAGT+GGG	+	chr4.2:19775940-19775959	None:intergenic	35.0%
!!!	TGTAGTTGGTTTTGGTAAAG+TGG	+	chr4.2:19775941-19775960	None:intergenic	35.0%
	AATGGAGGAAGATTTCCTTG+AGG	+	chr4.2:19775994-19776013	None:intergenic	40.0%
	ATAGAAGGGATAGTAGGCAA+TGG	+	chr4.2:19776012-19776031	None:intergenic	40.0%
	GGAATATTGGTAGGAAGACT+TGG	+	chr4.2:19776060-19776079	None:intergenic	40.0%
	GTGGGAATGGTAGGTAAATT+TGG	+	chr4.2:19775922-19775941	None:intergenic	40.0%
	TTAGGCATAGAAGGGATAGT+AGG	+	chr4.2:19776018-19776037	None:intergenic	40.0%
	TTTGGTAAAGTGGGAATGGT+AGG	+	chr4.2:19775931-19775950	None:intergenic	40.0%
!	AATGGTGGAATGTTGAGAGA+TGG	+	chr4.2:19776090-19776109	None:intergenic	40.0%
!	TGCAATGAAGGATTTGGTTG+AGG	+	chr4.2:19775827-19775846	None:intergenic	40.0%
!	TTTGGAATTGAAGGCAAAGG+TGG	+	chr4.2:19775967-19775986	None:intergenic	40.0%
!!	AAGGTGGTGGGGAAAAAAAT+GGG	+	chr4.2:19776170-19776189	None:intergenic	40.0%
!!	GGTGGTAATGTAGTTGGTTT+TGG	+	chr4.2:19775949-19775968	None:intergenic	40.0%
!!!	TGGTTTTGGTAAAGTGGGAA+TGG	+	chr4.2:19775935-19775954	None:intergenic	40.0%
	AAGTGGTTGGGATTGAAGGA+AGG	+	chr4.2:19776137-19776156	None:intergenic	45.0%
	AGTGGTGGCATAGTGAGTTT+AGG	+	chr4.2:19776036-19776055	None:intergenic	45.0%
	GGGAAAAAAATGGGAAGGAG+GGG	+	chr4.2:19776161-19776180	None:intergenic	45.0%
	GGGGAAAAAAATGGGAAGGA+GGG	+	chr4.2:19776162-19776181	None:intergenic	45.0%
	GTTGGGATTGAAGGAAGGTT+GGG	+	chr4.2:19776132-19776151	None:intergenic	45.0%
	TAAGGGCTAGAAGTTGAAGG+TGG	+	chr4.2:19776186-19776205	None:intergenic	45.0%
	TGGGGAAAAAAATGGGAAGG+AGG	+	chr4.2:19776163-19776182	None:intergenic	45.0%
	TGGTGGGGAAAAAAATGGGA+AGG	+	chr4.2:19776166-19776185	None:intergenic	45.0%
	TTGGTAGGAAGACTTGGAAG+TGG	+	chr4.2:19776054-19776073	None:intergenic	45.0%
!	GGCAAAGGTGGTAATGTAGT+TGG	+	chr4.2:19775955-19775974	None:intergenic	45.0%
!!	GAAGGTGGTGGGGAAAAAAA+TGG	+	chr4.2:19776171-19776190	None:intergenic	45.0%
	AAATGGGAAGGAGGGGAAAG+TGG	+	chr4.2:19776154-19776173	None:intergenic	50.0%
	AGGAAGGTTGGGAAGTGAAG+TGG	+	chr4.2:19776121-19776140	None:intergenic	50.0%
	GAAGGGATAGTAGGCAATGG+AGG	+	chr4.2:19776009-19776028	None:intergenic	50.0%
	GCAATAGATGGCGAGCTTCT+AGG	+	chr4.2:19775885-19775904	None:intergenic	50.0%
	GCTAGAAGTTGAAGGTGGTG+GGG	+	chr4.2:19776181-19776200	None:intergenic	50.0%
	GGCTAGAAGTTGAAGGTGGT+GGG	+	chr4.2:19776182-19776201	None:intergenic	50.0%
	GGGAAAGTGGTTGGGATTGA+AGG	+	chr4.2:19776141-19776160	None:intergenic	50.0%
	GGTTGGGATTGAAGGAAGGT+TGG	+	chr4.2:19776133-19776152	None:intergenic	50.0%
	GTAGGAAGACTTGGAAGTGG+TGG	+	chr4.2:19776051-19776070	None:intergenic	50.0%
	AGTGAAGTGGCTGCTGGCAA+TGG	+	chr4.2:19776108-19776127	None:intergenic	55.0%
	GGAAGGAGGGGAAAGTGGTT+GGG	+	chr4.2:19776149-19776168	None:intergenic	55.0%
	GGGCTAGAAGTTGAAGGTGG+TGG	+	chr4.2:19776183-19776202	None:intergenic	55.0%
	TTGGGAAGTGAAGTGGCTGC+TGG	+	chr4.2:19776114-19776133	None:intergenic	55.0%
	GAAGTGGCTGCTGGCAATGG+TGG	+	chr4.2:19776105-19776124	None:intergenic	60.0%
	GGGAAGGAGGGGAAAGTGGT+TGG	+	chr4.2:19776150-19776169	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	19775820	19776206	19775820	ID=MS.gene90209
chr4.2	mRNA	19775820	19776206	19775820	ID=MS.gene90209.t1;Parent=MS.gene90209
chr4.2	exon	19775820	19776206	19775820	ID=MS.gene90209.t1.exon1;Parent=MS.gene90209.t1
chr4.2	CDS	19775820	19776206	19775820	ID=cds.MS.gene90209.t1;Parent=MS.gene90209.t1

Gene Sequence

>MS.gene90209

ATGGCCTCAACCAAATCCTTCATTGCAGCTTTACTTCTTGTTGTCACAATGTCAAGCATGAGCCTAGAAGCTCGCCATCTATTGCAAACAACAACACAACCAAATTTACCTACCATTCCCACTTTACCAAAACCAACTACATTACCACCTTTGCCTTCAATTCCAAATATGCCTCAAGGAAATCTTCCTCCATTGCCTACTATCCCTTCTATGCCTAAACTCACTATGCCACCACTTCCAAGTCTTCCTACCAATATTCCAACAATTCCATCTCTCAACATTCCACCATTGCCAGCAGCCACTTCACTTCCCAACCTTCCTTCAATCCCAACCACTTTCCCCTCCTTCCCATTTTTTTCCCCACCACCTTCAACTTCTAGCCCTTAA

Protein sequence

>MS.gene90209.t1

MASTKSFIAALLLVVTMSSMSLEARHLLQTTTQPNLPTIPTLPKPTTLPPLPSIPNMPQGNLPPLPTIPSMPKLTMPPLPSLPTNIPTIPSLNIPPLPAATSLPNLPSIPTTFPSFPFFSPPPSTSSP