Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90959.t1 | KEH30541.1 | 76.9 | 260 | 43 | 4 | 1 | 258 | 6 | 250 | 6.20E-91 | 344 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90959.t1 | A0A072ULG4 | 76.9 | 260 | 43 | 4 | 1 | 258 | 1 | 245 | 4.4e-91 | 344.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene90959 | 0.818933 | 1.51E-52 | -1.69E-46 |
| MS.gene049452 | MS.gene90959 | 0.806085 | 9.76E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene90959 | 0.80048 | 1.41E-48 | -1.69E-46 |
| MS.gene051198 | MS.gene90959 | 0.82331 | 1.48E-53 | -1.69E-46 |
| MS.gene053079 | MS.gene90959 | 0.800788 | 1.22E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene90959 | 0.819521 | 1.11E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene90959 | 0.816125 | 6.50E-52 | -1.69E-46 |
| MS.gene056366 | MS.gene90959 | 0.804241 | 2.37E-49 | -1.69E-46 |
| MS.gene056776 | MS.gene90959 | 0.835829 | 1.34E-56 | -1.69E-46 |
| MS.gene057208 | MS.gene90959 | 0.841799 | 3.82E-58 | -1.69E-46 |
| MS.gene058009 | MS.gene90959 | 0.804061 | 2.59E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene90959 | 0.80432 | 2.29E-49 | -1.69E-46 |
| MS.gene058783 | MS.gene90959 | 0.806965 | 6.37E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene90959 | 0.809008 | 2.34E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene90959 | 0.803061 | 4.17E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90959.t1 | MTR_4g074140 | 83.077 | 260 | 27 | 4 | 1 | 258 | 6 | 250 | 4.70e-140 | 394 |
| MS.gene90959.t1 | MTR_4g074140 | 83.077 | 260 | 27 | 4 | 1 | 258 | 1 | 245 | 5.46e-140 | 393 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 58 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGCCGGAAATTGCCAATT+TGG | 0.274884 | 4.2:-34574688 | None:intergenic |
| TGAGCCAAATTGGCAATTTC+CGG | 0.286882 | 4.2:+34574684 | MS.gene90959:CDS |
| TCAATGACGCTGACGCTAAC+TGG | 0.319388 | 4.2:-34573956 | None:intergenic |
| GTCATTGATGGCATCGCTTA+TGG | 0.330311 | 4.2:+34573971 | MS.gene90959:CDS |
| AGATATGTTGGACGTCTTCT+TGG | 0.352253 | 4.2:+34574852 | MS.gene90959:CDS |
| GCATCATCGACCTTCGGAAT+TGG | 0.354610 | 4.2:-34574010 | None:intergenic |
| CATTGCCGGAGTCACTTTCA+TGG | 0.363002 | 4.2:-34574036 | None:intergenic |
| TAATGAAAATAAGAAGAAAA+AGG | 0.372353 | 4.2:+34574228 | MS.gene90959:CDS |
| GTAGACAGTGTGAGCCAAAT+TGG | 0.380599 | 4.2:+34574674 | MS.gene90959:CDS |
| AAAGAAACGCGGAAATTTAG+AGG | 0.388630 | 4.2:+34574288 | MS.gene90959:CDS |
| TTTGATGGCAATTGCAGAAC+AGG | 0.393940 | 4.2:+34574390 | MS.gene90959:CDS |
| TTTAGACCAACGGAATGAAA+TGG | 0.394245 | 4.2:+34574192 | MS.gene90959:CDS |
| CCTATTTGAGATGTGCTTGT+AGG | 0.401685 | 4.2:-34574817 | None:intergenic |
| TCTATAGAAAATGAAGCTAT+TGG | 0.404032 | 4.2:+34574076 | MS.gene90959:CDS |
| GGAATTACCTCCTCAGTTGC+TGG | 0.415477 | 4.2:-34574796 | None:intergenic |
| TAGTGCAAAAGGTGAGGTTA+AGG | 0.416681 | 4.2:+34574159 | MS.gene90959:CDS |
| GATATGTTGGACGTCTTCTT+GGG | 0.420094 | 4.2:+34574853 | MS.gene90959:CDS |
| AGTGTCTAGCGACGATGATA+TGG | 0.425121 | 4.2:+34574351 | MS.gene90959:CDS |
| CCTACAAGCACATCTCAAAT+AGG | 0.432958 | 4.2:+34574817 | MS.gene90959:CDS |
| ATTAGCTTCAACAACATTGC+CGG | 0.440034 | 4.2:-34574050 | None:intergenic |
| TCTCAAAGGTTTCTCAGTAA+TGG | 0.440318 | 4.2:-34574877 | None:intergenic |
| GTTAGCGTCAGCGTCATTGA+TGG | 0.440543 | 4.2:+34573959 | MS.gene90959:CDS |
| GGAATGAATAACTGTGTTGA+GGG | 0.446298 | 4.2:+34573899 | None:intergenic |
| CATCACCCATTTCATTCCGT+TGG | 0.460429 | 4.2:-34574198 | None:intergenic |
| GTTGACGGTTGATGATTTGA+TGG | 0.465634 | 4.2:+34574375 | MS.gene90959:CDS |
| GTTCCTGCTTCAGCTGTGGC+CGG | 0.472137 | 4.2:-34574703 | None:intergenic |
| AGTGCAAAAGGTGAGGTTAA+GGG | 0.475605 | 4.2:+34574160 | MS.gene90959:CDS |
| TGGCTTGCATCATCGACCTT+CGG | 0.485290 | 4.2:-34574016 | None:intergenic |
| AAGTCGAATTCTAGTGCAAA+AGG | 0.494931 | 4.2:+34574148 | MS.gene90959:CDS |
| AGTTACCATCATGGATGAAA+AGG | 0.495277 | 4.2:+34573933 | MS.gene90959:CDS |
| TTAGACCAACGGAATGAAAT+GGG | 0.495671 | 4.2:+34574193 | MS.gene90959:CDS |
| AGGCGATCAAATCGAAGGAT+GGG | 0.513205 | 4.2:+34574248 | MS.gene90959:CDS |
| CGACGATGATATGGAGTTGA+CGG | 0.527405 | 4.2:+34574360 | MS.gene90959:CDS |
| CGTTGTTCCTGCTTCAGCTG+TGG | 0.533603 | 4.2:-34574707 | None:intergenic |
| CGTTTCTTTATAGAACGTCT+AGG | 0.537766 | 4.2:-34574274 | None:intergenic |
| TTTCCGGCCACAGCTGAAGC+AGG | 0.538346 | 4.2:+34574700 | MS.gene90959:CDS |
| TCATCCTGCTCAAGATATGT+TGG | 0.547783 | 4.2:+34574840 | MS.gene90959:CDS |
| TACCTCCTCAGTTGCTGGAG+AGG | 0.547924 | 4.2:-34574791 | None:intergenic |
| GCAGTATGTCAAAGATTATG+AGG | 0.550398 | 4.2:+34574627 | MS.gene90959:intron |
| GCAAGAAGGAAACGATAAAG+AGG | 0.562260 | 4.2:+34574125 | MS.gene90959:CDS |
| GGAATTGGCATCATGCATGA+TGG | 0.562904 | 4.2:-34573995 | None:intergenic |
| ACGTCCAACATATCTTGAGC+AGG | 0.563117 | 4.2:-34574844 | None:intergenic |
| AGGAATGAATAACTGTGTTG+AGG | 0.565426 | 4.2:+34573898 | None:intergenic |
| AAGGCGATCAAATCGAAGGA+TGG | 0.570967 | 4.2:+34574247 | MS.gene90959:CDS |
| GCTTCCCTTACCGAATTAGA+CGG | 0.583689 | 4.2:-34574757 | None:intergenic |
| GGCGATCAAATCGAAGGATG+GGG | 0.592288 | 4.2:+34574249 | MS.gene90959:CDS |
| GAAGAAAATTGATTGCAAGA+AGG | 0.594859 | 4.2:+34574111 | MS.gene90959:CDS |
| GAAAACGCCAGTTACCATCA+TGG | 0.601249 | 4.2:+34573924 | MS.gene90959:CDS |
| GCAAGCCATGAAAGTGACTC+CGG | 0.612685 | 4.2:+34574031 | MS.gene90959:CDS |
| ATGCATGATGCCAATTCCGA+AGG | 0.620795 | 4.2:+34574000 | MS.gene90959:CDS |
| AATTTAGAGGATCTTAGCCA+TGG | 0.622615 | 4.2:+34574301 | MS.gene90959:CDS |
| TAATTCCTCTCCAGCAACTG+AGG | 0.627429 | 4.2:+34574786 | MS.gene90959:CDS |
| AAAACCGTCTAATTCGGTAA+GGG | 0.640099 | 4.2:+34574753 | MS.gene90959:CDS |
| TTCCTCTCCAGCAACTGAGG+AGG | 0.651343 | 4.2:+34574789 | MS.gene90959:CDS |
| GAATTCTAGTGCAAAAGGTG+AGG | 0.665140 | 4.2:+34574153 | MS.gene90959:CDS |
| ATACATCATCACTACCACCA+TGG | 0.727291 | 4.2:-34574318 | None:intergenic |
| TTAGAGGATCTTAGCCATGG+TGG | 0.730179 | 4.2:+34574304 | MS.gene90959:CDS |
| AGACGTTCTATAAAGAAACG+CGG | 0.745605 | 4.2:+34574277 | MS.gene90959:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGAAAATAAGAAGAAAA+AGG | + | chr4.2:34574228-34574247 | MS.gene90959:CDS | 15.0% |
| ! | GAAAAACAAATTTAGACCAA+CGG | + | chr4.2:34574182-34574201 | MS.gene90959:CDS | 25.0% |
| ! | TCTATAGAAAATGAAGCTAT+TGG | + | chr4.2:34574076-34574095 | MS.gene90959:CDS | 25.0% |
| AAAATTGTTCAAGCGGTTAT+AGG | - | chr4.2:34574596-34574615 | None:intergenic | 30.0% | |
| AAATTGTTCAAGCGGTTATA+GGG | - | chr4.2:34574595-34574614 | None:intergenic | 30.0% | |
| ATACGACAAAATTGTTCAAG+CGG | - | chr4.2:34574603-34574622 | None:intergenic | 30.0% | |
| GAACAAAAAACCGTCTAATT+CGG | + | chr4.2:34574747-34574766 | MS.gene90959:CDS | 30.0% | |
| GAAGAAAATTGATTGCAAGA+AGG | + | chr4.2:34574111-34574130 | MS.gene90959:CDS | 30.0% | |
| ! | GCAATCAATTTTCTTCACAT+TGG | - | chr4.2:34574107-34574126 | None:intergenic | 30.0% |
| AAAAACCGTCTAATTCGGTA+AGG | + | chr4.2:34574752-34574771 | MS.gene90959:CDS | 35.0% | |
| AAAACCGTCTAATTCGGTAA+GGG | + | chr4.2:34574753-34574772 | MS.gene90959:CDS | 35.0% | |
| AAACAATGTAAAAGCTACCG+TGG | - | chr4.2:34574453-34574472 | None:intergenic | 35.0% | |
| AAAGAAACGCGGAAATTTAG+AGG | + | chr4.2:34574288-34574307 | MS.gene90959:CDS | 35.0% | |
| AAGTCGAATTCTAGTGCAAA+AGG | + | chr4.2:34574148-34574167 | MS.gene90959:CDS | 35.0% | |
| AATATTGCTTCCCAAGACAA+TGG | + | chr4.2:34574543-34574562 | MS.gene90959:intron | 35.0% | |
| AATTTAGAGGATCTTAGCCA+TGG | + | chr4.2:34574301-34574320 | MS.gene90959:CDS | 35.0% | |
| AGACGTTCTATAAAGAAACG+CGG | + | chr4.2:34574277-34574296 | MS.gene90959:CDS | 35.0% | |
| AGTTACCATCATGGATGAAA+AGG | + | chr4.2:34573933-34573952 | MS.gene90959:CDS | 35.0% | |
| ATCACATCAACCATTGTCTT+GGG | - | chr4.2:34574556-34574575 | None:intergenic | 35.0% | |
| CGTTTCTTTATAGAACGTCT+AGG | - | chr4.2:34574277-34574296 | None:intergenic | 35.0% | |
| GCAGTATGTCAAAGATTATG+AGG | + | chr4.2:34574627-34574646 | MS.gene90959:intron | 35.0% | |
| TTAGACCAACGGAATGAAAT+GGG | + | chr4.2:34574193-34574212 | MS.gene90959:CDS | 35.0% | |
| TTTAGACCAACGGAATGAAA+TGG | + | chr4.2:34574192-34574211 | MS.gene90959:CDS | 35.0% | |
| ! | ATTAGCTTCAACAACATTGC+CGG | - | chr4.2:34574053-34574072 | None:intergenic | 35.0% |
| ! | TTTTCATCCATGATGGTAAC+TGG | - | chr4.2:34573934-34573953 | None:intergenic | 35.0% |
| AGATATGTTGGACGTCTTCT+TGG | + | chr4.2:34574852-34574871 | MS.gene90959:CDS | 40.0% | |
| AGTGCAAAAGGTGAGGTTAA+GGG | + | chr4.2:34574160-34574179 | MS.gene90959:CDS | 40.0% | |
| ATACATCATCACTACCACCA+TGG | - | chr4.2:34574321-34574340 | None:intergenic | 40.0% | |
| CATCACATCAACCATTGTCT+TGG | - | chr4.2:34574557-34574576 | None:intergenic | 40.0% | |
| CCTACAAGCACATCTCAAAT+AGG | + | chr4.2:34574817-34574836 | MS.gene90959:CDS | 40.0% | |
| GAAAAAGGCGATCAAATCGA+AGG | + | chr4.2:34574243-34574262 | MS.gene90959:CDS | 40.0% | |
| GAATTCTAGTGCAAAAGGTG+AGG | + | chr4.2:34574153-34574172 | MS.gene90959:CDS | 40.0% | |
| GATATGTTGGACGTCTTCTT+GGG | + | chr4.2:34574853-34574872 | MS.gene90959:CDS | 40.0% | |
| GCAAGAAGGAAACGATAAAG+AGG | + | chr4.2:34574125-34574144 | MS.gene90959:CDS | 40.0% | |
| GCTGATAATTCAAGCATCCA+CGG | + | chr4.2:34574433-34574452 | MS.gene90959:intron | 40.0% | |
| TAGTGCAAAAGGTGAGGTTA+AGG | + | chr4.2:34574159-34574178 | MS.gene90959:CDS | 40.0% | |
| TCATCCTGCTCAAGATATGT+TGG | + | chr4.2:34574840-34574859 | MS.gene90959:CDS | 40.0% | |
| TGAGCCAAATTGGCAATTTC+CGG | + | chr4.2:34574684-34574703 | MS.gene90959:CDS | 40.0% | |
| ! | CAAGACAATGGTTGATGTGA+TGG | + | chr4.2:34574555-34574574 | MS.gene90959:intron | 40.0% |
| ! | CCTATTTGAGATGTGCTTGT+AGG | - | chr4.2:34574820-34574839 | None:intergenic | 40.0% |
| ! | TTTGATGGCAATTGCAGAAC+AGG | + | chr4.2:34574390-34574409 | MS.gene90959:CDS | 40.0% |
| !! | GTTGACGGTTGATGATTTGA+TGG | + | chr4.2:34574375-34574394 | MS.gene90959:CDS | 40.0% |
| !!! | CGGTTTTTTGTTCTCACGAA+AGG | - | chr4.2:34574740-34574759 | None:intergenic | 40.0% |
| AAGGCGATCAAATCGAAGGA+TGG | + | chr4.2:34574247-34574266 | MS.gene90959:CDS | 45.0% | |
| ACGTCCAACATATCTTGAGC+AGG | - | chr4.2:34574847-34574866 | None:intergenic | 45.0% | |
| AGGCGATCAAATCGAAGGAT+GGG | + | chr4.2:34574248-34574267 | MS.gene90959:CDS | 45.0% | |
| AGTGTCTAGCGACGATGATA+TGG | + | chr4.2:34574351-34574370 | MS.gene90959:CDS | 45.0% | |
| ATGCATGATGCCAATTCCGA+AGG | + | chr4.2:34574000-34574019 | MS.gene90959:CDS | 45.0% | |
| CATCACCCATTTCATTCCGT+TGG | - | chr4.2:34574201-34574220 | None:intergenic | 45.0% | |
| CGACGATGATATGGAGTTGA+CGG | + | chr4.2:34574360-34574379 | MS.gene90959:CDS | 45.0% | |
| GAAAACGCCAGTTACCATCA+TGG | + | chr4.2:34573924-34573943 | MS.gene90959:CDS | 45.0% | |
| GCTTCCCTTACCGAATTAGA+CGG | - | chr4.2:34574760-34574779 | None:intergenic | 45.0% | |
| GGAATTGGCATCATGCATGA+TGG | - | chr4.2:34573998-34574017 | None:intergenic | 45.0% | |
| GTAGACAGTGTGAGCCAAAT+TGG | + | chr4.2:34574674-34574693 | MS.gene90959:CDS | 45.0% | |
| TAATTCCTCTCCAGCAACTG+AGG | + | chr4.2:34574786-34574805 | MS.gene90959:CDS | 45.0% | |
| TTAGAGGATCTTAGCCATGG+TGG | + | chr4.2:34574304-34574323 | MS.gene90959:CDS | 45.0% | |
| ! | GTCATTGATGGCATCGCTTA+TGG | + | chr4.2:34573971-34573990 | MS.gene90959:CDS | 45.0% |
| !!! | ACTGGCCTTTTCATCCATGA+TGG | - | chr4.2:34573941-34573960 | None:intergenic | 45.0% |
| GCAAGCCATGAAAGTGACTC+CGG | + | chr4.2:34574031-34574050 | MS.gene90959:CDS | 50.0% | |
| GCATCATCGACCTTCGGAAT+TGG | - | chr4.2:34574013-34574032 | None:intergenic | 50.0% | |
| GGAATTACCTCCTCAGTTGC+TGG | - | chr4.2:34574799-34574818 | None:intergenic | 50.0% | |
| GGCGATCAAATCGAAGGATG+GGG | + | chr4.2:34574249-34574268 | MS.gene90959:CDS | 50.0% | |
| GTGGCCGGAAATTGCCAATT+TGG | - | chr4.2:34574691-34574710 | None:intergenic | 50.0% | |
| GTTAGCGTCAGCGTCATTGA+TGG | + | chr4.2:34573959-34573978 | MS.gene90959:CDS | 50.0% | |
| TCAATGACGCTGACGCTAAC+TGG | - | chr4.2:34573959-34573978 | None:intergenic | 50.0% | |
| ! | CATTGCCGGAGTCACTTTCA+TGG | - | chr4.2:34574039-34574058 | None:intergenic | 50.0% |
| ! | TGGCTTGCATCATCGACCTT+CGG | - | chr4.2:34574019-34574038 | None:intergenic | 50.0% |
| CGTTGTTCCTGCTTCAGCTG+TGG | - | chr4.2:34574710-34574729 | None:intergenic | 55.0% | |
| TACCTCCTCAGTTGCTGGAG+AGG | - | chr4.2:34574794-34574813 | None:intergenic | 55.0% | |
| TTCCTCTCCAGCAACTGAGG+AGG | + | chr4.2:34574789-34574808 | MS.gene90959:CDS | 55.0% | |
| GTTCCTGCTTCAGCTGTGGC+CGG | - | chr4.2:34574706-34574725 | None:intergenic | 60.0% | |
| TTTCCGGCCACAGCTGAAGC+AGG | + | chr4.2:34574700-34574719 | MS.gene90959:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 34573902 | 34574897 | 34573902 | ID=MS.gene90959 |
| chr4.2 | mRNA | 34573902 | 34574897 | 34573902 | ID=MS.gene90959.t1;Parent=MS.gene90959 |
| chr4.2 | exon | 34573902 | 34574411 | 34573902 | ID=MS.gene90959.t1.exon1;Parent=MS.gene90959.t1 |
| chr4.2 | CDS | 34573902 | 34574411 | 34573902 | ID=cds.MS.gene90959.t1;Parent=MS.gene90959.t1 |
| chr4.2 | exon | 34574631 | 34574897 | 34574631 | ID=MS.gene90959.t1.exon2;Parent=MS.gene90959.t1 |
| chr4.2 | CDS | 34574631 | 34574897 | 34574631 | ID=cds.MS.gene90959.t1;Parent=MS.gene90959.t1 |
| Gene Sequence |
| Protein sequence |
