Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91347.t1 | XP_013469954.1 | 96.3 | 136 | 5 | 0 | 1 | 136 | 79 | 214 | 5.20E-65 | 256.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91347.t1 | A0A072VQC5 | 96.3 | 136 | 5 | 0 | 1 | 136 | 79 | 214 | 3.8e-65 | 256.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049757 | MS.gene91347 | 0.816229 | 6.16E-52 | -1.69E-46 |
| MS.gene052100 | MS.gene91347 | 0.825237 | 5.23E-54 | -1.69E-46 |
| MS.gene053912 | MS.gene91347 | 0.841985 | 3.41E-58 | -1.69E-46 |
| MS.gene056387 | MS.gene91347 | 0.812588 | 3.93E-51 | -1.69E-46 |
| MS.gene06097 | MS.gene91347 | 0.800715 | 1.27E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91347.t1 | MTR_1g105970 | 96.324 | 136 | 5 | 0 | 1 | 136 | 79 | 214 | 4.07e-94 | 270 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91347.t1 | AT1G50020 | 59.124 | 137 | 50 | 2 | 1 | 136 | 78 | 209 | 6.17e-52 | 164 |
Find 38 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCTCCTCGGCTAAATTAA+AGG | 0.278659 | 1.3:+72504238 | MS.gene91347:CDS |
| GGAACATTCTTCTTGGCATT+TGG | 0.330859 | 1.3:+72503334 | MS.gene91347:CDS |
| GAATCAGAGGGAACGGTCTA+TGG | 0.337234 | 1.3:+72502658 | MS.gene91347:CDS |
| ACTTGGAGGAACATTCTTCT+TGG | 0.347709 | 1.3:+72503327 | MS.gene91347:CDS |
| CTTCTGTGAATGAAGAAGAT+AGG | 0.362753 | 1.3:+72502585 | MS.gene91347:CDS |
| GATTACAAGCAAACCAACTA+TGG | 0.380799 | 1.3:+72502548 | MS.gene91347:CDS |
| CTTCCGTCGTGGACAAAACT+TGG | 0.396329 | 1.3:-72504144 | None:intergenic |
| TCATCTTCTAAGAGTGCATA+AGG | 0.401040 | 1.3:-72504201 | None:intergenic |
| CTCTTCATCAACTCCATAGT+TGG | 0.402993 | 1.3:-72502561 | None:intergenic |
| TTCTGTGAATGAAGAAGATA+GGG | 0.460643 | 1.3:+72502586 | MS.gene91347:CDS |
| AAACTTCTATTGACACTTGG+AGG | 0.515017 | 1.3:+72503313 | MS.gene91347:CDS |
| TAATCCTCAAGCAATGATGT+AGG | 0.515998 | 1.3:-72502530 | None:intergenic |
| AATCCTCAAGCAATGATGTA+GGG | 0.519413 | 1.3:-72502529 | None:intergenic |
| ACTATCACCTCCACAATATG+TGG | 0.522313 | 1.3:+72504176 | MS.gene91347:CDS |
| CTCTAGACCCCTCTCAACAT+TGG | 0.523903 | 1.3:-72502636 | None:intergenic |
| CATCTTCTAAGAGTGCATAA+GGG | 0.537509 | 1.3:-72504200 | None:intergenic |
| GCATAAGGGTCCACATATTG+TGG | 0.543635 | 1.3:-72504186 | None:intergenic |
| AGAGGGGTCTAGAGAATCAG+AGG | 0.544137 | 1.3:+72502645 | MS.gene91347:CDS |
| GTCTAGAGAATCAGAGGGAA+CGG | 0.571889 | 1.3:+72502651 | MS.gene91347:CDS |
| GACCCCTACATCATTGCTTG+AGG | 0.575241 | 1.3:+72502526 | MS.gene91347:CDS |
| ATGCACTCTTAGAAGATGAA+AGG | 0.586584 | 1.3:+72504205 | MS.gene91347:CDS |
| GCTAAACTTCTATTGACACT+TGG | 0.589619 | 1.3:+72503310 | MS.gene91347:CDS |
| AGTGTCAACCAATGTTGAGA+GGG | 0.601124 | 1.3:+72502628 | MS.gene91347:CDS |
| GCCACGGTTATGAGGATCAA+CGG | 0.602581 | 1.3:-72503358 | None:intergenic |
| CAACTATGGAGTTGATGAAG+AGG | 0.608576 | 1.3:+72502562 | MS.gene91347:CDS |
| CAGTGTCAACCAATGTTGAG+AGG | 0.610884 | 1.3:+72502627 | MS.gene91347:CDS |
| GGATATCTCAGATAGCTCCT+CGG | 0.629394 | 1.3:+72504226 | MS.gene91347:CDS |
| GTGACATCTTGCTTCCGTCG+TGG | 0.633182 | 1.3:-72504155 | None:intergenic |
| GCTGAACAGTGAAGGTCTTG+AGG | 0.634680 | 1.3:+72502682 | MS.gene91347:CDS |
| ATCCTCAAGCAATGATGTAG+GGG | 0.635911 | 1.3:-72502528 | None:intergenic |
| TCTATGGCGCTGAACAGTGA+AGG | 0.641145 | 1.3:+72502674 | MS.gene91347:CDS |
| GTCAATAGAAGTTTAGCTCG+AGG | 0.648431 | 1.3:-72503304 | None:intergenic |
| GAGGGGTCTAGAGAATCAGA+GGG | 0.670863 | 1.3:+72502646 | MS.gene91347:CDS |
| TAAGGGTCCACATATTGTGG+AGG | 0.678073 | 1.3:-72504183 | None:intergenic |
| AGAGCTTACGAAGTAAAGAG+CGG | 0.697103 | 1.3:-72503389 | None:intergenic |
| GTGTCAACCAATGTTGAGAG+GGG | 0.698061 | 1.3:+72502629 | MS.gene91347:CDS |
| ACCGTTGATCCTCATAACCG+TGG | 0.700978 | 1.3:+72503357 | MS.gene91347:CDS |
| TCTGTGAATGAAGAAGATAG+GGG | 0.715285 | 1.3:+72502587 | MS.gene91347:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTTACTACAATACAAATA+AGG | - | chr1.3:72503167-72503186 | None:intergenic | 15.0% |
| !!! | AGAATATACATTTTTTCTAA+AGG | - | chr1.3:72503638-72503657 | None:intergenic | 15.0% |
| !!! | TTTTACATACTTTCTAAATA+GGG | + | chr1.3:72503137-72503156 | MS.gene91347:intron | 15.0% |
| !! | ATAAATATGTAAGTGATTTC+TGG | - | chr1.3:72503266-72503285 | None:intergenic | 20.0% |
| !! | ATAGTACACATTTAATGATA+TGG | + | chr1.3:72503479-72503498 | MS.gene91347:intron | 20.0% |
| !! | TAATGTTAAACATAAACCAT+AGG | - | chr1.3:72503871-72503890 | None:intergenic | 20.0% |
| !! | TTACATATTTATCCATTTCA+GGG | + | chr1.3:72503274-72503293 | MS.gene91347:intron | 20.0% |
| !!! | GTTTTACATACTTTCTAAAT+AGG | + | chr1.3:72503136-72503155 | MS.gene91347:intron | 20.0% |
| !!! | TATATAAAAAATCGCTGATT+TGG | + | chr1.3:72502736-72502755 | MS.gene91347:intron | 20.0% |
| ! | CTTACATATTTATCCATTTC+AGG | + | chr1.3:72503273-72503292 | MS.gene91347:intron | 25.0% |
| ! | TATTGAAGGAAAGTTAACAA+TGG | - | chr1.3:72502713-72502732 | None:intergenic | 25.0% |
| ! | TGAAGTGAATAACAATTTGA+TGG | + | chr1.3:72503541-72503560 | MS.gene91347:intron | 25.0% |
| !!! | ATATTCTAGCTTATAACAGT+TGG | + | chr1.3:72503651-72503670 | MS.gene91347:intron | 25.0% |
| AACATAAACCATAGGATAGA+TGG | - | chr1.3:72503863-72503882 | None:intergenic | 30.0% | |
| ACATAAACCATAGGATAGAT+GGG | - | chr1.3:72503862-72503881 | None:intergenic | 30.0% | |
| ACTAAGACTTCAGAACATTT+AGG | + | chr1.3:72503595-72503614 | MS.gene91347:intron | 30.0% | |
| ATAAACAGCCAAATCAAGTT+TGG | - | chr1.3:72502876-72502895 | None:intergenic | 30.0% | |
| CAACGTTCTATATTACATGA+AGG | - | chr1.3:72504089-72504108 | None:intergenic | 30.0% | |
| CATGTAATATAGAACGTTGA+TGG | + | chr1.3:72504090-72504109 | MS.gene91347:intron | 30.0% | |
| TAACATCTTGATCTGATGTT+TGG | + | chr1.3:72502912-72502931 | MS.gene91347:intron | 30.0% | |
| TAGATTACTTAACACGCAAA+CGG | - | chr1.3:72503458-72503477 | None:intergenic | 30.0% | |
| TTCTGTGAATGAAGAAGATA+GGG | + | chr1.3:72502586-72502605 | MS.gene91347:CDS | 30.0% | |
| TTTGTGCTTGTATGAAATAG+TGG | + | chr1.3:72504051-72504070 | MS.gene91347:intron | 30.0% | |
| ! | AATAGTCTTCAGTTTTCAAC+AGG | - | chr1.3:72503934-72503953 | None:intergenic | 30.0% |
| ! | ATAGTCTTCAGTTTTCAACA+GGG | - | chr1.3:72503933-72503952 | None:intergenic | 30.0% |
| ! | ATCTGATGTTTGGAACTTAA+AGG | + | chr1.3:72502922-72502941 | MS.gene91347:intron | 30.0% |
| ! | GTTTTTGTGCATTCAATTCT+TGG | + | chr1.3:72503784-72503803 | MS.gene91347:intron | 30.0% |
| ! | TGTAAGCTGTATTTCTATGA+TGG | - | chr1.3:72503225-72503244 | None:intergenic | 30.0% |
| ! | TTGATTTTGTGTCTGTTGTT+TGG | + | chr1.3:72503725-72503744 | MS.gene91347:intron | 30.0% |
| !! | TCTTAGTTTTCCAATTGAGT+TGG | - | chr1.3:72503582-72503601 | None:intergenic | 30.0% |
| !!! | ATTTTTTCTAAAGGAAGCTG+AGG | - | chr1.3:72503629-72503648 | None:intergenic | 30.0% |
| !!! | CTGTTTTTGTTGCAGTATTT+CGG | + | chr1.3:72504120-72504139 | MS.gene91347:intron | 30.0% |
| AAAAAAATGCCACGGTTATG+AGG | - | chr1.3:72503369-72503388 | None:intergenic | 35.0% | |
| AAACTTCTATTGACACTTGG+AGG | + | chr1.3:72503313-72503332 | MS.gene91347:CDS | 35.0% | |
| AATCCTCAAGCAATGATGTA+GGG | - | chr1.3:72502532-72502551 | None:intergenic | 35.0% | |
| ACAGAACACATTACATTAGC+TGG | - | chr1.3:72503423-72503442 | None:intergenic | 35.0% | |
| ATGAGAATAGAGTTCTTCAG+CGG | - | chr1.3:72503028-72503047 | None:intergenic | 35.0% | |
| ATGCACTCTTAGAAGATGAA+AGG | + | chr1.3:72504205-72504224 | MS.gene91347:CDS | 35.0% | |
| CTCAATGTGAAGAATGAATG+AGG | - | chr1.3:72504027-72504046 | None:intergenic | 35.0% | |
| CTTCTGTGAATGAAGAAGAT+AGG | + | chr1.3:72502585-72502604 | MS.gene91347:CDS | 35.0% | |
| GATTACAAGCAAACCAACTA+TGG | + | chr1.3:72502548-72502567 | MS.gene91347:CDS | 35.0% | |
| GCTAAACTTCTATTGACACT+TGG | + | chr1.3:72503310-72503329 | MS.gene91347:CDS | 35.0% | |
| TAATCCTCAAGCAATGATGT+AGG | - | chr1.3:72502533-72502552 | None:intergenic | 35.0% | |
| TATTTATCCATTTCAGGGCT+TGG | + | chr1.3:72503279-72503298 | MS.gene91347:intron | 35.0% | |
| TCAAACCACCAAACTTGATT+TGG | + | chr1.3:72502865-72502884 | MS.gene91347:intron | 35.0% | |
| TCTGTGAATGAAGAAGATAG+GGG | + | chr1.3:72502587-72502606 | MS.gene91347:CDS | 35.0% | |
| TGAAGAATGAATGAGGATTG+AGG | - | chr1.3:72504020-72504039 | None:intergenic | 35.0% | |
| ! | ATTTTCTGTTTGCGTCATGT+TGG | + | chr1.3:72503969-72503988 | MS.gene91347:intron | 35.0% |
| ! | CATCTTCTAAGAGTGCATAA+GGG | - | chr1.3:72504203-72504222 | None:intergenic | 35.0% |
| ! | TCATCTTCTAAGAGTGCATA+AGG | - | chr1.3:72504204-72504223 | None:intergenic | 35.0% |
| ! | TTGGAACTTAAAGGTTCTGA+TGG | + | chr1.3:72502931-72502950 | MS.gene91347:intron | 35.0% |
| !! | CTTGTATGAAATAGTGGACA+TGG | + | chr1.3:72504057-72504076 | MS.gene91347:intron | 35.0% |
| !! | GTTGACACTGTTTTTCTGTT+CGG | - | chr1.3:72502617-72502636 | None:intergenic | 35.0% |
| !! | TTGACACTGTTTTTCTGTTC+GGG | - | chr1.3:72502616-72502635 | None:intergenic | 35.0% |
| !!! | GTTTTCCAATTGAGTTGGTT+AGG | - | chr1.3:72503577-72503596 | None:intergenic | 35.0% |
| AACAGCCAAATCAAGTTTGG+TGG | - | chr1.3:72502873-72502892 | None:intergenic | 40.0% | |
| AAGAGCGGAAAAAAATGCCA+CGG | - | chr1.3:72503377-72503396 | None:intergenic | 40.0% | |
| ACTATCACCTCCACAATATG+TGG | + | chr1.3:72504176-72504195 | MS.gene91347:CDS | 40.0% | |
| ACTTGGAGGAACATTCTTCT+TGG | + | chr1.3:72503327-72503346 | MS.gene91347:CDS | 40.0% | |
| AGAGCTTACGAAGTAAAGAG+CGG | - | chr1.3:72503392-72503411 | None:intergenic | 40.0% | |
| AGTGTCAACCAATGTTGAGA+GGG | + | chr1.3:72502628-72502647 | MS.gene91347:CDS | 40.0% | |
| ATCCTCAAGCAATGATGTAG+GGG | - | chr1.3:72502531-72502550 | None:intergenic | 40.0% | |
| CAGAACCTAACCAACTCAAT+TGG | + | chr1.3:72503569-72503588 | MS.gene91347:intron | 40.0% | |
| CTCTTCATCAACTCCATAGT+TGG | - | chr1.3:72502564-72502583 | None:intergenic | 40.0% | |
| GCATTGTTTGTTCTGCTCTA+AGG | + | chr1.3:72502789-72502808 | MS.gene91347:intron | 40.0% | |
| GGAACATTCTTCTTGGCATT+TGG | + | chr1.3:72503334-72503353 | MS.gene91347:CDS | 40.0% | |
| TACAGCTTACATGCTTCCTA+TGG | + | chr1.3:72503234-72503253 | MS.gene91347:intron | 40.0% | |
| TACTTAACACGCAAACGGTA+CGG | - | chr1.3:72503453-72503472 | None:intergenic | 40.0% | |
| TTGTCTTCCCATCTATCCTA+TGG | + | chr1.3:72503852-72503871 | MS.gene91347:intron | 40.0% | |
| ! | GTCAATAGAAGTTTAGCTCG+AGG | - | chr1.3:72503307-72503326 | None:intergenic | 40.0% |
| !! | CAACTATGGAGTTGATGAAG+AGG | + | chr1.3:72502562-72502581 | MS.gene91347:CDS | 40.0% |
| !! | TGATTTCTGGTGACTACCAT+AGG | - | chr1.3:72503253-72503272 | None:intergenic | 40.0% |
| CAGTGTCAACCAATGTTGAG+AGG | + | chr1.3:72502627-72502646 | MS.gene91347:CDS | 45.0% | |
| GCATAAGGGTCCACATATTG+TGG | - | chr1.3:72504189-72504208 | None:intergenic | 45.0% | |
| GGATATCTCAGATAGCTCCT+CGG | + | chr1.3:72504226-72504245 | MS.gene91347:CDS | 45.0% | |
| GTCTAGAGAATCAGAGGGAA+CGG | + | chr1.3:72502651-72502670 | MS.gene91347:CDS | 45.0% | |
| GTGTCAACCAATGTTGAGAG+GGG | + | chr1.3:72502629-72502648 | MS.gene91347:CDS | 45.0% | |
| TAAGGGTCCACATATTGTGG+AGG | - | chr1.3:72504186-72504205 | None:intergenic | 45.0% | |
| !!! | TTTTTTATATATATTATTGA+AGG | - | chr1.3:72502727-72502746 | None:intergenic | 5.0% |
| ACCGTTGATCCTCATAACCG+TGG | + | chr1.3:72503357-72503376 | MS.gene91347:CDS | 50.0% | |
| AGAGGGGTCTAGAGAATCAG+AGG | + | chr1.3:72502645-72502664 | MS.gene91347:CDS | 50.0% | |
| CTCTAGACCCCTCTCAACAT+TGG | - | chr1.3:72502639-72502658 | None:intergenic | 50.0% | |
| CTTCCGTCGTGGACAAAACT+TGG | - | chr1.3:72504147-72504166 | None:intergenic | 50.0% | |
| GAATCAGAGGGAACGGTCTA+TGG | + | chr1.3:72502658-72502677 | MS.gene91347:CDS | 50.0% | |
| GACCCCTACATCATTGCTTG+AGG | + | chr1.3:72502526-72502545 | MS.gene91347:CDS | 50.0% | |
| GAGGGGTCTAGAGAATCAGA+GGG | + | chr1.3:72502646-72502665 | MS.gene91347:CDS | 50.0% | |
| GCCACGGTTATGAGGATCAA+CGG | - | chr1.3:72503361-72503380 | None:intergenic | 50.0% | |
| GCTGAACAGTGAAGGTCTTG+AGG | + | chr1.3:72502682-72502701 | MS.gene91347:CDS | 50.0% | |
| ! | TCTATGGCGCTGAACAGTGA+AGG | + | chr1.3:72502674-72502693 | MS.gene91347:CDS | 50.0% |
| !! | GGACCAAGTTTTGTCCACGA+CGG | + | chr1.3:72504141-72504160 | MS.gene91347:CDS | 50.0% |
| GTGACATCTTGCTTCCGTCG+TGG | - | chr1.3:72504158-72504177 | None:intergenic | 55.0% | |
| ! | CGAGGTACCAAGCCCTGAAA+TGG | - | chr1.3:72503289-72503308 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 72502524 | 72504257 | 72502524 | ID=MS.gene91347 |
| chr1.3 | mRNA | 72502524 | 72504257 | 72502524 | ID=MS.gene91347.t1;Parent=MS.gene91347 |
| chr1.3 | exon | 72502524 | 72502703 | 72502524 | ID=MS.gene91347.t1.exon1;Parent=MS.gene91347.t1 |
| chr1.3 | CDS | 72502524 | 72502703 | 72502524 | ID=cds.MS.gene91347.t1;Parent=MS.gene91347.t1 |
| chr1.3 | exon | 72503295 | 72503402 | 72503295 | ID=MS.gene91347.t1.exon2;Parent=MS.gene91347.t1 |
| chr1.3 | CDS | 72503295 | 72503402 | 72503295 | ID=cds.MS.gene91347.t1;Parent=MS.gene91347.t1 |
| chr1.3 | exon | 72504135 | 72504257 | 72504135 | ID=MS.gene91347.t1.exon3;Parent=MS.gene91347.t1 |
| chr1.3 | CDS | 72504135 | 72504257 | 72504135 | ID=cds.MS.gene91347.t1;Parent=MS.gene91347.t1 |
| Gene Sequence |
| Protein sequence |
