Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92554.t1 | XP_013454934.1 | 85.9 | 320 | 28 | 6 | 1 | 318 | 1 | 305 | 1.70E-130 | 475.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92554.t1 | A0A072UH33 | 85.9 | 320 | 28 | 6 | 1 | 318 | 1 | 305 | 1.2e-130 | 475.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05045 | MS.gene92554 | 0.809518 | 1.82E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene92554 | 0.811971 | 5.36E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene92554 | 0.800088 | 1.70E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene92554 | 0.8507 | 1.43E-60 | -1.69E-46 |
| MS.gene05364 | MS.gene92554 | 0.825348 | 4.92E-54 | -1.69E-46 |
| MS.gene054775 | MS.gene92554 | 0.806212 | 9.18E-50 | -1.69E-46 |
| MS.gene056215 | MS.gene92554 | 0.813053 | 3.11E-51 | -1.69E-46 |
| MS.gene057693 | MS.gene92554 | 0.806871 | 6.66E-50 | -1.69E-46 |
| MS.gene058783 | MS.gene92554 | 0.801536 | 8.60E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene92554 | 0.801505 | 8.73E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92554.t1 | MTR_4g019840 | 86.562 | 320 | 26 | 6 | 1 | 318 | 1 | 305 | 0.0 | 544 |
| MS.gene92554.t1 | MTR_0027s0090 | 29.577 | 355 | 190 | 12 | 9 | 318 | 51 | 390 | 3.49e-22 | 96.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTTTCGTTTCTCAAATT+AGG | 0.099863 | 4.2:+82402313 | None:intergenic |
| TTAGGTGTTGGAAGTGATTA+TGG | 0.259586 | 4.2:-82401913 | MS.gene92554:intron |
| TTCTTTCGTTTCTCAAATTA+GGG | 0.263492 | 4.2:+82402314 | None:intergenic |
| AATAACAAAGCCGGATTTCC+TGG | 0.285031 | 4.2:-82402340 | MS.gene92554:CDS |
| AAAGCATGAACAACTCTTTC+AGG | 0.307734 | 4.2:+82402110 | None:intergenic |
| TTCTTCCTCCAGGGATATTT+CGG | 0.316300 | 4.2:+82402446 | None:intergenic |
| TTTCTCAAATTAGGGTTTCC+AGG | 0.367756 | 4.2:+82402322 | None:intergenic |
| TCAGTGTTCCTTCTTCCTCC+AGG | 0.391507 | 4.2:+82402436 | None:intergenic |
| ATTAGGGTTTCCAGGAAATC+CGG | 0.430658 | 4.2:+82402330 | None:intergenic |
| CAGTGTTCCTTCTTCCTCCA+GGG | 0.484157 | 4.2:+82402437 | None:intergenic |
| CAGAGGTGAACCGTGAGATC+CGG | 0.494994 | 4.2:-82401852 | MS.gene92554:CDS |
| GTGATTGAAACGTCGTCGTA+AGG | 0.507589 | 4.2:+82403053 | None:intergenic |
| TGTCTTCATCTGCATCTGCA+TGG | 0.512850 | 4.2:-82402387 | MS.gene92554:CDS |
| AAGAGTGGTTGAACTTGAAA+CGG | 0.521960 | 4.2:+82403079 | None:intergenic |
| GGAAATTACCGAAATATCCC+TGG | 0.523195 | 4.2:-82402454 | MS.gene92554:CDS |
| TTGAATCGTAAATTTGAAGA+GGG | 0.533843 | 4.2:-82402089 | MS.gene92554:CDS |
| GCTATTTCACAGAGAAGAAG+TGG | 0.536018 | 4.2:-82402475 | MS.gene92554:CDS |
| AGATCCGGCTTCCTCAAGAA+TGG | 0.537735 | 4.2:-82401837 | MS.gene92554:CDS |
| CTGCAAGTTCTGCTTCAAAA+CGG | 0.546551 | 4.2:+82402860 | None:intergenic |
| GATCTGCTACATGCAGCTGC+AGG | 0.549527 | 4.2:-82402825 | MS.gene92554:CDS |
| CTTGAGGAAGCCGGATCTCA+CGG | 0.554510 | 4.2:+82401842 | None:intergenic |
| AGAAACGAAAGAAATGGTAC+TGG | 0.560342 | 4.2:-82402304 | MS.gene92554:CDS |
| GCATGGAGTTCTGTGAAGAA+TGG | 0.561312 | 4.2:-82402370 | MS.gene92554:CDS |
| AAGTCCATTCTTGAGGAAGC+CGG | 0.565560 | 4.2:+82401833 | None:intergenic |
| GAAATATCCCTGGAGGAAGA+AGG | 0.566843 | 4.2:-82402444 | MS.gene92554:CDS |
| GAAGAGGGCATGATGATGAG+AGG | 0.567680 | 4.2:-82402074 | MS.gene92554:CDS |
| GATTCTGAGTGTGGAATCCA+TGG | 0.568409 | 4.2:+82402727 | None:intergenic |
| GGAAGCAACAATAACAAAGC+CGG | 0.570578 | 4.2:-82402349 | MS.gene92554:CDS |
| AATTTGAGAAACGAAAGAAA+TGG | 0.570863 | 4.2:-82402310 | MS.gene92554:CDS |
| AGCAGAACTTGCAGAGAGCG+AGG | 0.573031 | 4.2:-82402851 | MS.gene92554:CDS |
| TTTGAATCGTAAATTTGAAG+AGG | 0.586109 | 4.2:-82402090 | MS.gene92554:CDS |
| AGGAAGAAGGAACACTGAAA+CGG | 0.589211 | 4.2:-82402431 | MS.gene92554:CDS |
| TTGAGATGTTGAAGAAGCAG+TGG | 0.595071 | 4.2:-82402525 | MS.gene92554:CDS |
| CATCAATAAGTCCATTCTTG+AGG | 0.595722 | 4.2:+82401826 | None:intergenic |
| CTCATCTCTCCACAACAACA+AGG | 0.596113 | 4.2:-82403006 | MS.gene92554:intron |
| AGTCATTTGACGAGTTAACT+CGG | 0.596535 | 4.2:+82403031 | None:intergenic |
| GATGATGATGATTCTGAGTG+TGG | 0.607664 | 4.2:+82402718 | None:intergenic |
| AGCTACGCGAAGAGTTCAAG+CGG | 0.634893 | 4.2:-82402891 | MS.gene92554:CDS |
| CGTGAAGCAGCAGCTGACAG+AGG | 0.646842 | 4.2:-82401869 | MS.gene92554:CDS |
| GCAGTGGTTGAAGCAACGAG+AGG | 0.691118 | 4.2:-82402509 | MS.gene92554:CDS |
| AATTACCGAAATATCCCTGG+AGG | 0.707791 | 4.2:-82402451 | MS.gene92554:CDS |
| TCAGTCGCATTAACAACACG+AGG | 0.752315 | 4.2:+82402271 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATACAAATTAATTAATTAC+AGG | + | chr4.2:82402991-82403010 | None:intergenic | 10.0% |
| !!! | AATTTTTTGTTTATGTTTTT+TGG | - | chr4.2:82402822-82402841 | MS.gene92554:CDS | 10.0% |
| !!! | ATTTTTTGTTTATGTTTTTT+GGG | - | chr4.2:82402823-82402842 | MS.gene92554:CDS | 10.0% |
| !!! | AAAGTTTTTTTGTATTGATT+TGG | - | chr4.2:82403010-82403029 | MS.gene92554:CDS | 15.0% |
| !!! | AAGTTTTTTTGTATTGATTT+GGG | - | chr4.2:82403011-82403030 | MS.gene92554:CDS | 15.0% |
| !!! | TTTTTTGTTTATGTTTTTTG+GGG | - | chr4.2:82402824-82402843 | MS.gene92554:CDS | 15.0% |
| !!! | AGTTTTTTTGTATTGATTTG+GGG | - | chr4.2:82403012-82403031 | MS.gene92554:CDS | 20.0% |
| !!! | TTTTTGTTTATGTTTTTTGG+GGG | - | chr4.2:82402825-82402844 | MS.gene92554:CDS | 20.0% |
| ! | AATTTGAGAAACGAAAGAAA+TGG | - | chr4.2:82402673-82402692 | MS.gene92554:intron | 25.0% |
| ! | ACTGAATCAAGAAAAAATTC+AGG | - | chr4.2:82402730-82402749 | MS.gene92554:CDS | 25.0% |
| ! | TTCTTTCGTTTCTCAAATTA+GGG | + | chr4.2:82402672-82402691 | None:intergenic | 25.0% |
| ! | TTGAATCGTAAATTTGAAGA+GGG | - | chr4.2:82402894-82402913 | MS.gene92554:CDS | 25.0% |
| ! | TTTCTTTCGTTTCTCAAATT+AGG | + | chr4.2:82402673-82402692 | None:intergenic | 25.0% |
| ! | TTTGAATCGTAAATTTGAAG+AGG | - | chr4.2:82402893-82402912 | MS.gene92554:CDS | 25.0% |
| !!! | GGAACTTCAAATTTTGAAAA+TGG | - | chr4.2:82402960-82402979 | MS.gene92554:intron | 25.0% |
| !!! | GTTTTTTTGTATTGATTTGG+GGG | - | chr4.2:82403013-82403032 | MS.gene92554:CDS | 25.0% |
| !!! | TCTATTTTTGTTTCTTCACA+GGG | - | chr4.2:82402053-82402072 | MS.gene92554:CDS | 25.0% |
| !!! | TGATGTGTTTGTTGTATTTT+AGG | - | chr4.2:82403052-82403071 | MS.gene92554:CDS | 25.0% |
| TGTAGGTAGTAAAAATGTGA+TGG | - | chr4.2:82402848-82402867 | MS.gene92554:CDS | 30.0% | |
| ! | GAAGAAGAAGAAGCTTTTTA+CGG | - | chr4.2:82402215-82402234 | MS.gene92554:intron | 30.0% |
| ! | GATGAAAGATTTTTCGTCTT+CGG | + | chr4.2:82401862-82401881 | None:intergenic | 30.0% |
| !!! | CTCTATTTTTGTTTCTTCAC+AGG | - | chr4.2:82402052-82402071 | MS.gene92554:CDS | 30.0% |
| !!! | GTTTGTTGTATTTTAGGTGT+TGG | - | chr4.2:82403058-82403077 | MS.gene92554:CDS | 30.0% |
| !!! | TTTTAGTTACCTTGTTGTTG+TGG | + | chr4.2:82401989-82402008 | None:intergenic | 30.0% |
| AAAGCATGAACAACTCTTTC+AGG | + | chr4.2:82402876-82402895 | None:intergenic | 35.0% | |
| AAGAGTGGTTGAACTTGAAA+CGG | + | chr4.2:82401907-82401926 | None:intergenic | 35.0% | |
| AGAAACGAAAGAAATGGTAC+TGG | - | chr4.2:82402679-82402698 | MS.gene92554:CDS | 35.0% | |
| AGTCATTTGACGAGTTAACT+CGG | + | chr4.2:82401955-82401974 | None:intergenic | 35.0% | |
| TTTCTCAAATTAGGGTTTCC+AGG | + | chr4.2:82402664-82402683 | None:intergenic | 35.0% | |
| ! | ATGGATTTTCTCAGCAAAAG+CGG | - | chr4.2:82403089-82403108 | MS.gene92554:CDS | 35.0% |
| !! | ACGGTTTTTCAGAGTAATGT+TGG | - | chr4.2:82402571-82402590 | MS.gene92554:intron | 35.0% |
| !! | ATTTTTGTTTCTTCACAGGG+TGG | - | chr4.2:82402056-82402075 | MS.gene92554:CDS | 35.0% |
| !! | CAGCAGAAGAATCGTTTTTA+TGG | - | chr4.2:82402939-82402958 | MS.gene92554:intron | 35.0% |
| !! | TTAGGTGTTGGAAGTGATTA+TGG | - | chr4.2:82403070-82403089 | MS.gene92554:CDS | 35.0% |
| !!! | TTTATGTTTTTTGGGGGTGT+AGG | - | chr4.2:82402831-82402850 | MS.gene92554:CDS | 35.0% |
| AATAACAAAGCCGGATTTCC+TGG | - | chr4.2:82402643-82402662 | MS.gene92554:intron | 40.0% | |
| AATTACCGAAATATCCCTGG+AGG | - | chr4.2:82402532-82402551 | MS.gene92554:intron | 40.0% | |
| AGGAAGAAGGAACACTGAAA+CGG | - | chr4.2:82402552-82402571 | MS.gene92554:intron | 40.0% | |
| ATTAGGGTTTCCAGGAAATC+CGG | + | chr4.2:82402656-82402675 | None:intergenic | 40.0% | |
| CTGCAAGTTCTGCTTCAAAA+CGG | + | chr4.2:82402126-82402145 | None:intergenic | 40.0% | |
| GGAAATTACCGAAATATCCC+TGG | - | chr4.2:82402529-82402548 | MS.gene92554:CDS | 40.0% | |
| GGAAGCAACAATAACAAAGC+CGG | - | chr4.2:82402634-82402653 | MS.gene92554:intron | 40.0% | |
| TTCTTCCTCCAGGGATATTT+CGG | + | chr4.2:82402540-82402559 | None:intergenic | 40.0% | |
| TTGAGATGTTGAAGAAGCAG+TGG | - | chr4.2:82402458-82402477 | MS.gene92554:CDS | 40.0% | |
| ! | GATGATGATGATTCTGAGTG+TGG | + | chr4.2:82402268-82402287 | None:intergenic | 40.0% |
| ! | GCTATTTCACAGAGAAGAAG+TGG | - | chr4.2:82402508-82402527 | MS.gene92554:CDS | 40.0% |
| !! | GAATCCATGGTGCTTTTTAG+GGG | + | chr4.2:82402246-82402265 | None:intergenic | 40.0% |
| !! | GGAATCCATGGTGCTTTTTA+GGG | + | chr4.2:82402247-82402266 | None:intergenic | 40.0% |
| !! | TGGAATCCATGGTGCTTTTT+AGG | + | chr4.2:82402248-82402267 | None:intergenic | 40.0% |
| !! | TTTACCCCTAAAAAGCACCA+TGG | - | chr4.2:82402239-82402258 | MS.gene92554:intron | 40.0% |
| AAGTCCATTCTTGAGGAAGC+CGG | + | chr4.2:82403153-82403172 | None:intergenic | 45.0% | |
| CTCATCTCTCCACAACAACA+AGG | - | chr4.2:82401977-82401996 | MS.gene92554:intron | 45.0% | |
| GAAATATCCCTGGAGGAAGA+AGG | - | chr4.2:82402539-82402558 | MS.gene92554:intron | 45.0% | |
| GATTCTGAGTGTGGAATCCA+TGG | + | chr4.2:82402259-82402278 | None:intergenic | 45.0% | |
| GCATGGAGTTCTGTGAAGAA+TGG | - | chr4.2:82402613-82402632 | MS.gene92554:intron | 45.0% | |
| GTCGTCGTAAGGAAAAAGAG+TGG | + | chr4.2:82401922-82401941 | None:intergenic | 45.0% | |
| GTGATTGAAACGTCGTCGTA+AGG | + | chr4.2:82401933-82401952 | None:intergenic | 45.0% | |
| TCAGTCGCATTAACAACACG+AGG | + | chr4.2:82402715-82402734 | None:intergenic | 45.0% | |
| TGTCTTCATCTGCATCTGCA+TGG | - | chr4.2:82402596-82402615 | MS.gene92554:intron | 45.0% | |
| !!! | TCACAGGGTGGTTTTGAGAA+TGG | - | chr4.2:82402068-82402087 | MS.gene92554:CDS | 45.0% |
| AGATCCGGCTTCCTCAAGAA+TGG | - | chr4.2:82403146-82403165 | MS.gene92554:CDS | 50.0% | |
| AGCTACGCGAAGAGTTCAAG+CGG | - | chr4.2:82402092-82402111 | MS.gene92554:CDS | 50.0% | |
| CAGTGTTCCTTCTTCCTCCA+GGG | + | chr4.2:82402549-82402568 | None:intergenic | 50.0% | |
| TCAGTGTTCCTTCTTCCTCC+AGG | + | chr4.2:82402550-82402569 | None:intergenic | 50.0% | |
| ! | GAAGAGGGCATGATGATGAG+AGG | - | chr4.2:82402909-82402928 | MS.gene92554:CDS | 50.0% |
| AGCAGAACTTGCAGAGAGCG+AGG | - | chr4.2:82402132-82402151 | MS.gene92554:CDS | 55.0% | |
| CAGAGGTGAACCGTGAGATC+CGG | - | chr4.2:82403131-82403150 | MS.gene92554:CDS | 55.0% | |
| CTTGAGGAAGCCGGATCTCA+CGG | + | chr4.2:82403144-82403163 | None:intergenic | 55.0% | |
| GATCTGCTACATGCAGCTGC+AGG | - | chr4.2:82402158-82402177 | MS.gene92554:intron | 55.0% | |
| GCAGTGGTTGAAGCAACGAG+AGG | - | chr4.2:82402474-82402493 | MS.gene92554:CDS | 55.0% | |
| CGTGAAGCAGCAGCTGACAG+AGG | - | chr4.2:82403114-82403133 | MS.gene92554:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 82401828 | 82403177 | 82401828 | ID=MS.gene92554 |
| chr4.2 | mRNA | 82401828 | 82403177 | 82401828 | ID=MS.gene92554.t1;Parent=MS.gene92554 |
| chr4.2 | exon | 82403007 | 82403177 | 82403007 | ID=MS.gene92554.t1.exon1;Parent=MS.gene92554.t1 |
| chr4.2 | CDS | 82403007 | 82403177 | 82403007 | ID=cds.MS.gene92554.t1;Parent=MS.gene92554.t1 |
| chr4.2 | exon | 82402674 | 82402931 | 82402674 | ID=MS.gene92554.t1.exon2;Parent=MS.gene92554.t1 |
| chr4.2 | CDS | 82402674 | 82402931 | 82402674 | ID=cds.MS.gene92554.t1;Parent=MS.gene92554.t1 |
| chr4.2 | exon | 82402254 | 82402548 | 82402254 | ID=MS.gene92554.t1.exon3;Parent=MS.gene92554.t1 |
| chr4.2 | CDS | 82402254 | 82402548 | 82402254 | ID=cds.MS.gene92554.t1;Parent=MS.gene92554.t1 |
| chr4.2 | exon | 82402024 | 82402152 | 82402024 | ID=MS.gene92554.t1.exon4;Parent=MS.gene92554.t1 |
| chr4.2 | CDS | 82402024 | 82402152 | 82402024 | ID=cds.MS.gene92554.t1;Parent=MS.gene92554.t1 |
| chr4.2 | exon | 82401828 | 82401931 | 82401828 | ID=MS.gene92554.t1.exon5;Parent=MS.gene92554.t1 |
| chr4.2 | CDS | 82401828 | 82401931 | 82401828 | ID=cds.MS.gene92554.t1;Parent=MS.gene92554.t1 |
| Gene Sequence |
| Protein sequence |
