Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92791.t1 | XP_003623667.1 | 92.4 | 211 | 16 | 0 | 1 | 211 | 1 | 211 | 5.60E-90 | 340.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92791.t1 | G7KZ69 | 92.4 | 211 | 16 | 0 | 1 | 211 | 1 | 211 | 4.0e-90 | 340.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene92791 | 0.800538 | 1.38E-48 | -1.69E-46 |
| MS.gene050128 | MS.gene92791 | 0.803145 | 4.01E-49 | -1.69E-46 |
| MS.gene050762 | MS.gene92791 | 0.802528 | 5.38E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene92791 | 0.802697 | 4.96E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene92791 | 0.819374 | 1.20E-52 | -1.69E-46 |
| MS.gene052340 | MS.gene92791 | 0.831986 | 1.22E-55 | -1.69E-46 |
| MS.gene053073 | MS.gene92791 | 0.835823 | 1.34E-56 | -1.69E-46 |
| MS.gene05316 | MS.gene92791 | 0.818377 | 2.02E-52 | -1.69E-46 |
| MS.gene053577 | MS.gene92791 | 0.805115 | 1.56E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene92791 | 0.806198 | 9.24E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene92791 | 0.836683 | 8.10E-57 | -1.69E-46 |
| MS.gene056659 | MS.gene92791 | 0.813945 | 1.98E-51 | -1.69E-46 |
| MS.gene056719 | MS.gene92791 | 0.809924 | 1.49E-50 | -1.69E-46 |
| MS.gene058949 | MS.gene92791 | 0.810295 | 1.24E-50 | -1.69E-46 |
| MS.gene059285 | MS.gene92791 | 0.800984 | 1.12E-48 | -1.69E-46 |
| MS.gene059894 | MS.gene92791 | 0.811369 | 7.25E-51 | -1.69E-46 |
| MS.gene060495 | MS.gene92791 | 0.815015 | 1.15E-51 | -1.69E-46 |
| MS.gene060496 | MS.gene92791 | 0.810502 | 1.12E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92791.t1 | MTR_7g074250 | 92.417 | 211 | 16 | 0 | 1 | 211 | 1 | 211 | 9.84e-128 | 358 |
| MS.gene92791.t1 | MTR_8g005060 | 35.602 | 191 | 118 | 2 | 21 | 211 | 7 | 192 | 3.42e-32 | 115 |
| MS.gene92791.t1 | MTR_8g028475 | 36.275 | 204 | 121 | 5 | 11 | 211 | 22 | 219 | 8.36e-25 | 97.4 |
| MS.gene92791.t1 | MTR_2g088610 | 26.486 | 185 | 125 | 4 | 29 | 211 | 9 | 184 | 2.47e-14 | 68.9 |
| MS.gene92791.t1 | MTR_5g005950 | 29.012 | 162 | 109 | 4 | 50 | 211 | 35 | 190 | 1.21e-12 | 64.3 |
| MS.gene92791.t1 | MTR_5g009130 | 25.698 | 179 | 125 | 3 | 19 | 197 | 9 | 179 | 1.42e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92791.t1 | AT3G05975 | 33.690 | 187 | 123 | 1 | 25 | 211 | 4 | 189 | 1.32e-35 | 124 |
| MS.gene92791.t1 | AT3G54200 | 31.579 | 228 | 136 | 2 | 3 | 211 | 9 | 235 | 1.09e-19 | 84.3 |
| MS.gene92791.t1 | AT2G46150 | 24.020 | 204 | 151 | 4 | 10 | 211 | 20 | 221 | 1.44e-14 | 70.1 |
| MS.gene92791.t1 | AT4G23930 | 24.309 | 181 | 124 | 2 | 32 | 205 | 13 | 187 | 2.27e-11 | 60.8 |
Find 22 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCCTTATTCTTGCAGATT+TGG | 0.252406 | 7.4:-30772888 | None:intergenic |
| TCATCAAGGCTTGTTCTTCT+TGG | 0.289565 | 7.4:-30772309 | None:intergenic |
| AATGGGTGGTCAACTCTCTT+TGG | 0.325009 | 7.4:+30772750 | MS.gene92791:CDS |
| AAGCCAAATCTGCAAGAATA+AGG | 0.332506 | 7.4:+30772885 | MS.gene92791:CDS |
| CCAAGAAAAGTAACTCTTCC+AGG | 0.349074 | 7.4:-30772790 | None:intergenic |
| GAAGCAGTTACTAGGATTCC+TGG | 0.355010 | 7.4:+30772772 | MS.gene92791:CDS |
| GCTGAAACAACCGTTGCTTT+TGG | 0.387060 | 7.4:-30772451 | None:intergenic |
| TCTCTTTGGAAGCAGTTACT+AGG | 0.401657 | 7.4:+30772764 | MS.gene92791:CDS |
| GTTGTTTCAGCTACTTTAGA+AGG | 0.419593 | 7.4:+30772463 | MS.gene92791:CDS |
| ATGCTAATTTATCAGCTTGA+AGG | 0.435205 | 7.4:-30772699 | None:intergenic |
| ATGTATAAAGGTGTTGAAGT+TGG | 0.503450 | 7.4:+30772610 | MS.gene92791:CDS |
| AAATTAGCATCCAATGTGAC+AGG | 0.508296 | 7.4:+30772712 | MS.gene92791:CDS |
| CTGCATGTGTGAAGCTTACA+CGG | 0.524124 | 7.4:-30772570 | None:intergenic |
| TGCTAATTTATCAGCTTGAA+GGG | 0.525961 | 7.4:-30772698 | None:intergenic |
| TTGAGTTGTATGTCAATAGC+AGG | 0.526566 | 7.4:-30772508 | None:intergenic |
| GGAGACACAGATATATATCC+TGG | 0.534807 | 7.4:+30772631 | MS.gene92791:CDS |
| GCTTGAAGGGTAAGTCTACA+AGG | 0.570776 | 7.4:-30772685 | None:intergenic |
| CCTGGTTTGATTGCTGCAAG+AGG | 0.591703 | 7.4:+30772649 | MS.gene92791:CDS |
| GTAAGCTTCACACATGCAGA+TGG | 0.594756 | 7.4:+30772574 | MS.gene92791:CDS |
| CCTCTTGCAGCAATCAAACC+AGG | 0.602161 | 7.4:-30772649 | None:intergenic |
| GCCAAAACAACCAAAAGCAA+CGG | 0.624220 | 7.4:+30772441 | MS.gene92791:CDS |
| ATAGCAGGAAATGTTAGACG+AGG | 0.747015 | 7.4:-30772493 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTAATTGTCAATTTGTTTT+TGG | + | chr7.4:30772841-30772860 | MS.gene92791:CDS | 20.0% |
| !!! | ACTAGTCTTTTGATGTATAA+AGG | + | chr7.4:30772598-30772617 | MS.gene92791:CDS | 25.0% |
| !!! | AGGTTTTTTAGGTGATTTAA+TGG | + | chr7.4:30772732-30772751 | MS.gene92791:CDS | 25.0% |
| !!! | GGTTTTTTAGGTGATTTAAT+GGG | + | chr7.4:30772733-30772752 | MS.gene92791:CDS | 25.0% |
| ATGCTAATTTATCAGCTTGA+AGG | - | chr7.4:30772702-30772721 | None:intergenic | 30.0% | |
| TGCTAATTTATCAGCTTGAA+GGG | - | chr7.4:30772701-30772720 | None:intergenic | 30.0% | |
| TTGATGAATCCACCAAAAAA+GGG | + | chr7.4:30772325-30772344 | MS.gene92791:CDS | 30.0% | |
| !! | ATGTATAAAGGTGTTGAAGT+TGG | + | chr7.4:30772610-30772629 | MS.gene92791:CDS | 30.0% |
| !!! | TGTTTTTGGTTTTCCTGATA+TGG | + | chr7.4:30772855-30772874 | MS.gene92791:CDS | 30.0% |
| !!! | TTTTTAGGTGATTTAATGGG+TGG | + | chr7.4:30772736-30772755 | MS.gene92791:CDS | 30.0% |
| AAATTAGCATCCAATGTGAC+AGG | + | chr7.4:30772712-30772731 | MS.gene92791:CDS | 35.0% | |
| AAGCCAAATCTGCAAGAATA+AGG | + | chr7.4:30772885-30772904 | MS.gene92791:CDS | 35.0% | |
| ACCTAAAAAACCTGTCACAT+TGG | - | chr7.4:30772725-30772744 | None:intergenic | 35.0% | |
| CTTGATGAATCCACCAAAAA+AGG | + | chr7.4:30772324-30772343 | MS.gene92791:CDS | 35.0% | |
| ! | GTTGTTTCAGCTACTTTAGA+AGG | + | chr7.4:30772463-30772482 | MS.gene92791:CDS | 35.0% |
| ! | TTGAGTTGTATGTCAATAGC+AGG | - | chr7.4:30772511-30772530 | None:intergenic | 35.0% |
| !! | TAGCCTTATTCTTGCAGATT+TGG | - | chr7.4:30772891-30772910 | None:intergenic | 35.0% |
| !!! | CATCTGTTTCTTCCCTTTTT+TGG | - | chr7.4:30772340-30772359 | None:intergenic | 35.0% |
| !!! | CTTTTTTGGTGGATTCATCA+AGG | - | chr7.4:30772326-30772345 | None:intergenic | 35.0% |
| !!! | TCCAATGTGACAGGTTTTTT+AGG | + | chr7.4:30772721-30772740 | MS.gene92791:CDS | 35.0% |
| !!! | TGGTTGTTTTGGCTTGAAAA+CGG | - | chr7.4:30772434-30772453 | None:intergenic | 35.0% |
| !!! | TTTTGGCTTGAAAACGGTTA+AGG | - | chr7.4:30772428-30772447 | None:intergenic | 35.0% |
| ATAGCAGGAAATGTTAGACG+AGG | - | chr7.4:30772496-30772515 | None:intergenic | 40.0% | |
| CCAAGAAAAGTAACTCTTCC+AGG | - | chr7.4:30772793-30772812 | None:intergenic | 40.0% | |
| GCCAAAACAACCAAAAGCAA+CGG | + | chr7.4:30772441-30772460 | MS.gene92791:CDS | 40.0% | |
| GGAGACACAGATATATATCC+TGG | + | chr7.4:30772631-30772650 | MS.gene92791:CDS | 40.0% | |
| ! | TCATCAAGGCTTGTTCTTCT+TGG | - | chr7.4:30772312-30772331 | None:intergenic | 40.0% |
| ! | TCTCTTTGGAAGCAGTTACT+AGG | + | chr7.4:30772764-30772783 | MS.gene92791:CDS | 40.0% |
| ! | TGGCTTTTGATCTCCATATC+AGG | - | chr7.4:30772871-30772890 | None:intergenic | 40.0% |
| !!! | ACCGTTGCTTTTGGTTGTTT+TGG | - | chr7.4:30772445-30772464 | None:intergenic | 40.0% |
| !!! | CCTGGAAGAGTTACTTTTCT+TGG | + | chr7.4:30772790-30772809 | MS.gene92791:CDS | 40.0% |
| !!! | CTGTTTCTTCCCTTTTTTGG+TGG | - | chr7.4:30772337-30772356 | None:intergenic | 40.0% |
| AATGGGTGGTCAACTCTCTT+TGG | + | chr7.4:30772750-30772769 | MS.gene92791:CDS | 45.0% | |
| CTGCATGTGTGAAGCTTACA+CGG | - | chr7.4:30772573-30772592 | None:intergenic | 45.0% | |
| GAAGCAGTTACTAGGATTCC+TGG | + | chr7.4:30772772-30772791 | MS.gene92791:CDS | 45.0% | |
| GCTTGAAGGGTAAGTCTACA+AGG | - | chr7.4:30772688-30772707 | None:intergenic | 45.0% | |
| GTAAGCTTCACACATGCAGA+TGG | + | chr7.4:30772574-30772593 | MS.gene92791:CDS | 45.0% | |
| !! | GCTGAAACAACCGTTGCTTT+TGG | - | chr7.4:30772454-30772473 | None:intergenic | 45.0% |
| CCTCTTGCAGCAATCAAACC+AGG | - | chr7.4:30772652-30772671 | None:intergenic | 50.0% | |
| ! | CCTGGTTTGATTGCTGCAAG+AGG | + | chr7.4:30772649-30772668 | MS.gene92791:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 30772283 | 30772918 | 30772283 | ID=MS.gene92791 |
| chr7.4 | mRNA | 30772283 | 30772918 | 30772283 | ID=MS.gene92791.t1;Parent=MS.gene92791 |
| chr7.4 | exon | 30772283 | 30772918 | 30772283 | ID=MS.gene92791.t1.exon1;Parent=MS.gene92791.t1 |
| chr7.4 | CDS | 30772283 | 30772918 | 30772283 | ID=cds.MS.gene92791.t1;Parent=MS.gene92791.t1 |
| Gene Sequence |
| Protein sequence |
