Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93622.t1 | GAU17735.1 | 88.1 | 159 | 19 | 0 | 1 | 159 | 181 | 339 | 2.70E-76 | 294.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93622.t1 | A2XRZ6 | 71.4 | 161 | 43 | 2 | 1 | 158 | 193 | 353 | 4.5e-64 | 245.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93622.t1 | A0A2Z6LKJ7 | 88.1 | 159 | 19 | 0 | 1 | 159 | 181 | 339 | 2.0e-76 | 294.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049669 | MS.gene93622 | 0.813949 | 1.98E-51 | -1.69E-46 |
| MS.gene050953 | MS.gene93622 | 0.801436 | 9.01E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene93622 | 0.82528 | 5.11E-54 | -1.69E-46 |
| MS.gene052389 | MS.gene93622 | 0.807866 | 4.10E-50 | -1.69E-46 |
| MS.gene052426 | MS.gene93622 | 0.808557 | 2.92E-50 | -1.69E-46 |
| MS.gene052586 | MS.gene93622 | 0.806769 | 7.00E-50 | -1.69E-46 |
| MS.gene054083 | MS.gene93622 | 0.815775 | 7.78E-52 | -1.69E-46 |
| MS.gene054093 | MS.gene93622 | 0.830765 | 2.44E-55 | -1.69E-46 |
| MS.gene054479 | MS.gene93622 | 0.813429 | 2.57E-51 | -1.69E-46 |
| MS.gene054826 | MS.gene93622 | 0.801756 | 7.75E-49 | -1.69E-46 |
| MS.gene055225 | MS.gene93622 | 0.840678 | 7.52E-58 | -1.69E-46 |
| MS.gene055701 | MS.gene93622 | 0.844135 | 9.12E-59 | -1.69E-46 |
| MS.gene057156 | MS.gene93622 | -0.810644 | 1.04E-50 | -1.69E-46 |
| MS.gene057194 | MS.gene93622 | 0.805783 | 1.13E-49 | -1.69E-46 |
| MS.gene060879 | MS.gene93622 | 0.810875 | 9.27E-51 | -1.69E-46 |
| MS.gene060998 | MS.gene93622 | 0.811566 | 6.57E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93622.t1 | MTR_7g021680 | 88.050 | 159 | 19 | 0 | 1 | 159 | 181 | 339 | 1.05e-100 | 293 |
| MS.gene93622.t1 | MTR_7g021820 | 88.050 | 159 | 19 | 0 | 1 | 159 | 181 | 339 | 1.05e-100 | 293 |
| MS.gene93622.t1 | MTR_7g021850 | 78.616 | 159 | 33 | 1 | 1 | 159 | 182 | 339 | 2.16e-90 | 267 |
| MS.gene93622.t1 | MTR_7g021825 | 95.082 | 122 | 6 | 0 | 1 | 122 | 39 | 160 | 6.35e-85 | 246 |
| MS.gene93622.t1 | MTR_7g021670 | 90.909 | 110 | 10 | 0 | 50 | 159 | 144 | 253 | 9.97e-69 | 209 |
| MS.gene93622.t1 | MTR_7g114990 | 48.718 | 156 | 73 | 4 | 1 | 152 | 192 | 344 | 2.88e-51 | 167 |
| MS.gene93622.t1 | MTR_7g114980 | 50.641 | 156 | 72 | 3 | 1 | 152 | 194 | 348 | 2.94e-51 | 167 |
| MS.gene93622.t1 | MTR_1g102750 | 50.641 | 156 | 72 | 3 | 1 | 152 | 185 | 339 | 1.08e-50 | 165 |
| MS.gene93622.t1 | MTR_7g115010 | 48.734 | 158 | 74 | 4 | 1 | 152 | 173 | 329 | 9.69e-50 | 162 |
| MS.gene93622.t1 | MTR_7g114970 | 44.937 | 158 | 77 | 4 | 1 | 151 | 44 | 198 | 1.85e-44 | 145 |
| MS.gene93622.t1 | MTR_7g114970 | 44.937 | 158 | 77 | 4 | 1 | 151 | 92 | 246 | 1.34e-43 | 144 |
| MS.gene93622.t1 | MTR_7g114970 | 44.937 | 158 | 77 | 4 | 1 | 151 | 186 | 340 | 6.15e-43 | 145 |
| MS.gene93622.t1 | MTR_1g102750 | 57.843 | 102 | 42 | 1 | 1 | 102 | 185 | 285 | 3.40e-39 | 135 |
| MS.gene93622.t1 | MTR_7g114980 | 55.000 | 100 | 44 | 1 | 1 | 100 | 194 | 292 | 5.97e-35 | 124 |
| MS.gene93622.t1 | MTR_7g114970 | 51.485 | 101 | 47 | 2 | 1 | 100 | 186 | 285 | 1.09e-31 | 115 |
| MS.gene93622.t1 | MTR_1g102740 | 48.571 | 105 | 50 | 1 | 52 | 152 | 3 | 107 | 2.25e-31 | 109 |
| MS.gene93622.t1 | MTR_7g114970 | 51.000 | 100 | 47 | 2 | 1 | 99 | 186 | 284 | 1.19e-30 | 112 |
| MS.gene93622.t1 | MTR_7g067855 | 35.172 | 145 | 55 | 4 | 47 | 152 | 10 | 154 | 7.07e-24 | 91.7 |
| MS.gene93622.t1 | MTR_3g449790 | 37.500 | 128 | 75 | 3 | 7 | 134 | 228 | 350 | 2.85e-16 | 75.1 |
| MS.gene93622.t1 | MTR_3g449790 | 37.500 | 128 | 75 | 3 | 7 | 134 | 234 | 356 | 3.02e-16 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93622.t1 | AT1G60730 | 72.671 | 161 | 42 | 1 | 1 | 159 | 185 | 345 | 1.06e-82 | 247 |
| MS.gene93622.t1 | AT1G60730 | 72.671 | 161 | 42 | 1 | 1 | 159 | 205 | 365 | 1.76e-82 | 248 |
| MS.gene93622.t1 | AT1G60680 | 70.000 | 160 | 46 | 1 | 1 | 158 | 186 | 345 | 1.51e-80 | 242 |
| MS.gene93622.t1 | AT1G60710 | 68.944 | 161 | 48 | 1 | 1 | 159 | 185 | 345 | 3.42e-80 | 241 |
| MS.gene93622.t1 | AT1G60690 | 67.702 | 161 | 50 | 1 | 1 | 159 | 185 | 345 | 1.50e-79 | 239 |
| MS.gene93622.t1 | AT1G10810 | 65.190 | 158 | 53 | 1 | 1 | 156 | 133 | 290 | 4.52e-74 | 224 |
| MS.gene93622.t1 | AT1G10810 | 65.190 | 158 | 53 | 1 | 1 | 156 | 185 | 342 | 5.69e-74 | 225 |
| MS.gene93622.t1 | AT1G60750 | 64.375 | 160 | 38 | 2 | 1 | 158 | 187 | 329 | 2.90e-68 | 210 |
| MS.gene93622.t1 | AT1G60680 | 71.429 | 49 | 14 | 0 | 1 | 49 | 186 | 234 | 1.73e-20 | 85.1 |
| MS.gene93622.t1 | AT1G10810 | 64.815 | 54 | 19 | 0 | 1 | 54 | 185 | 238 | 4.44e-20 | 84.0 |
| MS.gene93622.t1 | AT1G06690 | 33.600 | 125 | 78 | 3 | 10 | 134 | 248 | 367 | 1.22e-12 | 64.7 |
Find 31 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGATTCAAGTTCTCAATTT+TGG | 0.160230 | 7.3:-77735907 | None:intergenic |
| TCATAATGATTTATTTCTTA+TGG | 0.227099 | 7.3:-77736082 | None:intergenic |
| TCAAGTTCTCCCATTTCTTC+TGG | 0.258309 | 7.3:-77735960 | None:intergenic |
| ATACTCCACCACTCTCTTCT+TGG | 0.280266 | 7.3:+77736048 | MS.gene93622:CDS |
| TTTCTTAGGGAACTTGGAAT+TGG | 0.323334 | 7.3:+77735353 | MS.gene93622:intron |
| GAAACTAACACCAGAAGAAA+TGG | 0.357383 | 7.3:+77735950 | MS.gene93622:CDS |
| AAGACGTAATTCCAACTTGC+AGG | 0.402309 | 7.3:+77735072 | MS.gene93622:CDS |
| TGCAGTACATCCCTTCTTTG+TGG | 0.420403 | 7.3:-77735820 | None:intergenic |
| ATATAGTCTGATTCTGCTGC+AGG | 0.450000 | 7.3:-77735776 | None:intergenic |
| GTTGCTTGACAACTTGCAAC+AGG | 0.458094 | 7.3:+77735427 | MS.gene93622:CDS |
| CAATGAACTGGCCACAAAGA+AGG | 0.472154 | 7.3:+77735809 | MS.gene93622:CDS |
| CATCTCAGCTTGCATTGGCA+TGG | 0.479749 | 7.3:+77735841 | MS.gene93622:CDS |
| TCGGGCACACGTCGTTTCCT+TGG | 0.481328 | 7.3:-77735872 | None:intergenic |
| ATTTGACAAAGTCAATGAAC+TGG | 0.487530 | 7.3:+77735797 | MS.gene93622:CDS |
| TAAATATCACACCTGCAAGT+TGG | 0.505103 | 7.3:-77735083 | None:intergenic |
| AAACTAACACCAGAAGAAAT+GGG | 0.505399 | 7.3:+77735951 | MS.gene93622:CDS |
| AACGACGTGTGCCCGATACC+TGG | 0.512377 | 7.3:+77735879 | MS.gene93622:CDS |
| TACTGCATCTCAGCTTGCAT+TGG | 0.515172 | 7.3:+77735836 | MS.gene93622:CDS |
| AATGAACTGGCCACAAAGAA+GGG | 0.518216 | 7.3:+77735810 | MS.gene93622:CDS |
| GAAAAGAATCCTCGCTCAAG+AGG | 0.539199 | 7.3:-77735392 | None:intergenic |
| GAGAACTTGAATCAAAACAT+TGG | 0.571832 | 7.3:+77735915 | MS.gene93622:CDS |
| TAAGAGTGGTAGATATGTAG+AGG | 0.597868 | 7.3:+77736001 | MS.gene93622:CDS |
| TTAGCAAATGCTGTTAAGAG+TGG | 0.598227 | 7.3:+77735987 | MS.gene93622:CDS |
| ACTTGCAACAGGATGACTAC+AGG | 0.598957 | 7.3:+77735438 | MS.gene93622:CDS |
| ATGTAGAGGATACAAGTACA+TGG | 0.611228 | 7.3:+77736015 | MS.gene93622:CDS |
| GCATATAGTCCTCTTGAGCG+AGG | 0.639719 | 7.3:+77735383 | MS.gene93622:CDS |
| GCTTTCCAAGAAGAGAGTGG+TGG | 0.651055 | 7.3:-77736053 | None:intergenic |
| AATGTGGTCAAGAGATGTCG+AGG | 0.654297 | 7.3:+77735049 | None:intergenic |
| GCAACAGGATGACTACAGGA+AGG | 0.666822 | 7.3:+77735442 | MS.gene93622:CDS |
| TTGGCATGGCTTCATCACCA+AGG | 0.680145 | 7.3:+77735855 | MS.gene93622:CDS |
| GCAGCTTTCCAAGAAGAGAG+TGG | 0.687488 | 7.3:-77736056 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTATAATTTTCTTTCTTA+GGG | + | chr7.3:77735340-77735359 | MS.gene93622:intron | 15.0% |
| !! | TTTCTTCCATAAAACAATTT+GGG | + | chr7.3:77735621-77735640 | MS.gene93622:intron | 20.0% |
| !!! | ATTTTACTAACATGCTTAAA+AGG | - | chr7.3:77735721-77735740 | None:intergenic | 20.0% |
| !!! | GAGTTATAATTTTCTTTCTT+AGG | + | chr7.3:77735339-77735358 | MS.gene93622:intron | 20.0% |
| !!! | TTTTCTTCCATAAAACAATT+TGG | + | chr7.3:77735620-77735639 | MS.gene93622:intron | 20.0% |
| ! | ATAACACCAACAATTCAAAT+TGG | - | chr7.3:77735296-77735315 | None:intergenic | 25.0% |
| ! | ATGTTAGTAAAATATGTTGC+AGG | + | chr7.3:77735728-77735747 | MS.gene93622:intron | 25.0% |
| ! | TCATTGATGTATGACATTAA+AGG | - | chr7.3:77735571-77735590 | None:intergenic | 25.0% |
| ! | TTGTATCAGATAGAGAAAAA+TGG | - | chr7.3:77735137-77735156 | None:intergenic | 25.0% |
| !!! | TTGATTCAAGTTCTCAATTT+TGG | - | chr7.3:77735910-77735929 | None:intergenic | 25.0% |
| AAACTAACACCAGAAGAAAT+GGG | + | chr7.3:77735951-77735970 | MS.gene93622:CDS | 30.0% | |
| ATGCTTCCAATTTGAATTGT+TGG | + | chr7.3:77735287-77735306 | MS.gene93622:intron | 30.0% | |
| ATTTGACAAAGTCAATGAAC+TGG | + | chr7.3:77735797-77735816 | MS.gene93622:CDS | 30.0% | |
| CATGCTTAAAAGGAAGAATA+TGG | - | chr7.3:77735711-77735730 | None:intergenic | 30.0% | |
| CCATAAAACAATTTGGGAAT+AGG | + | chr7.3:77735627-77735646 | MS.gene93622:intron | 30.0% | |
| GAGAACTTGAATCAAAACAT+TGG | + | chr7.3:77735915-77735934 | MS.gene93622:CDS | 30.0% | |
| ! | ATTCAAATTGGAAGCATGTT+TGG | - | chr7.3:77735284-77735303 | None:intergenic | 30.0% |
| ! | ATTTTCTTTCTTAGGGAACT+TGG | + | chr7.3:77735347-77735366 | MS.gene93622:intron | 30.0% |
| !! | ATTCAAGTTCTCAATTTTGG+TGG | - | chr7.3:77735907-77735926 | None:intergenic | 30.0% |
| !! | CACAACCATGGATTATTTTA+GGG | + | chr7.3:77735544-77735563 | MS.gene93622:intron | 30.0% |
| !! | CCTATTCCCAAATTGTTTTA+TGG | - | chr7.3:77735630-77735649 | None:intergenic | 30.0% |
| !! | TCACAACCATGGATTATTTT+AGG | + | chr7.3:77735543-77735562 | MS.gene93622:intron | 30.0% |
| AAGGACCCTAAAATAATCCA+TGG | - | chr7.3:77735552-77735571 | None:intergenic | 35.0% | |
| ATGTAGAGGATACAAGTACA+TGG | + | chr7.3:77736015-77736034 | MS.gene93622:CDS | 35.0% | |
| GAAACTAACACCAGAAGAAA+TGG | + | chr7.3:77735950-77735969 | MS.gene93622:CDS | 35.0% | |
| TAAATATCACACCTGCAAGT+TGG | - | chr7.3:77735086-77735105 | None:intergenic | 35.0% | |
| TAAGAGTGGTAGATATGTAG+AGG | + | chr7.3:77736001-77736020 | MS.gene93622:CDS | 35.0% | |
| TTAGCAAATGCTGTTAAGAG+TGG | + | chr7.3:77735987-77736006 | MS.gene93622:CDS | 35.0% | |
| TTTCTTAGGGAACTTGGAAT+TGG | + | chr7.3:77735353-77735372 | MS.gene93622:intron | 35.0% | |
| AAGACGTAATTCCAACTTGC+AGG | + | chr7.3:77735072-77735091 | MS.gene93622:CDS | 40.0% | |
| AATGAACTGGCCACAAAGAA+GGG | + | chr7.3:77735810-77735829 | MS.gene93622:CDS | 40.0% | |
| ATATAGTCTGATTCTGCTGC+AGG | - | chr7.3:77735779-77735798 | None:intergenic | 40.0% | |
| GGGAATAGGTGTAACAGTAA+TGG | + | chr7.3:77735641-77735660 | MS.gene93622:intron | 40.0% | |
| TCAAGTTCTCCCATTTCTTC+TGG | - | chr7.3:77735963-77735982 | None:intergenic | 40.0% | |
| TGCTGCTAGTGAACACAATT+CGG | + | chr7.3:77735678-77735697 | MS.gene93622:intron | 40.0% | |
| ! | TGAGCGAGGATTCTTTTCAT+CGG | + | chr7.3:77735397-77735416 | MS.gene93622:CDS | 40.0% |
| !! | ATTTTGGTGGTTCCAGGTAT+CGG | - | chr7.3:77735894-77735913 | None:intergenic | 40.0% |
| !! | TGTTCGTGACGTTTTAGTGT+CGG | + | chr7.3:77735255-77735274 | MS.gene93622:intron | 40.0% |
| !! | TTCTCAATTTTGGTGGTTCC+AGG | - | chr7.3:77735900-77735919 | None:intergenic | 40.0% |
| !!! | AGGTTTTCAGGTTGAAATCG+AGG | - | chr7.3:77735759-77735778 | None:intergenic | 40.0% |
| ACTTGCAACAGGATGACTAC+AGG | + | chr7.3:77735438-77735457 | MS.gene93622:CDS | 45.0% | |
| ATACTCCACCACTCTCTTCT+TGG | + | chr7.3:77736048-77736067 | MS.gene93622:CDS | 45.0% | |
| CAATGAACTGGCCACAAAGA+AGG | + | chr7.3:77735809-77735828 | MS.gene93622:CDS | 45.0% | |
| CATGGTTGTGAATCACTGCT+AGG | - | chr7.3:77735534-77735553 | None:intergenic | 45.0% | |
| CTAGCAGTGATTCACAACCA+TGG | + | chr7.3:77735532-77735551 | MS.gene93622:intron | 45.0% | |
| GAAAAGAATCCTCGCTCAAG+AGG | - | chr7.3:77735395-77735414 | None:intergenic | 45.0% | |
| GATGACTACAGGAAGGTTTG+TGG | + | chr7.3:77735449-77735468 | MS.gene93622:intron | 45.0% | |
| GCTGCTAGTGAACACAATTC+GGG | + | chr7.3:77735679-77735698 | MS.gene93622:intron | 45.0% | |
| GTTGCTTGACAACTTGCAAC+AGG | + | chr7.3:77735427-77735446 | MS.gene93622:CDS | 45.0% | |
| TACTGCATCTCAGCTTGCAT+TGG | + | chr7.3:77735836-77735855 | MS.gene93622:CDS | 45.0% | |
| TGCAGTACATCCCTTCTTTG+TGG | - | chr7.3:77735823-77735842 | None:intergenic | 45.0% | |
| ! | GAGCGAGGATTCTTTTCATC+GGG | + | chr7.3:77735398-77735417 | MS.gene93622:CDS | 45.0% |
| ! | TGATTCTGCTGCAGGTTTTC+AGG | - | chr7.3:77735771-77735790 | None:intergenic | 45.0% |
| !! | TTTTGGTGGTTCCAGGTATC+GGG | - | chr7.3:77735893-77735912 | None:intergenic | 45.0% |
| GCAACAGGATGACTACAGGA+AGG | + | chr7.3:77735442-77735461 | MS.gene93622:CDS | 50.0% | |
| GCAGCTTTCCAAGAAGAGAG+TGG | - | chr7.3:77736059-77736078 | None:intergenic | 50.0% | |
| GCATATAGTCCTCTTGAGCG+AGG | + | chr7.3:77735383-77735402 | MS.gene93622:CDS | 50.0% | |
| GCTTTCCAAGAAGAGAGTGG+TGG | - | chr7.3:77736056-77736075 | None:intergenic | 50.0% | |
| TTGGCATGGCTTCATCACCA+AGG | + | chr7.3:77735855-77735874 | MS.gene93622:CDS | 50.0% | |
| ! | CATCTCAGCTTGCATTGGCA+TGG | + | chr7.3:77735841-77735860 | MS.gene93622:CDS | 50.0% |
| AACGACGTGTGCCCGATACC+TGG | + | chr7.3:77735879-77735898 | MS.gene93622:CDS | 60.0% | |
| TCGGGCACACGTCGTTTCCT+TGG | - | chr7.3:77735875-77735894 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 77735050 | 77736094 | 77735050 | ID=MS.gene93622 |
| chr7.3 | mRNA | 77735050 | 77736094 | 77735050 | ID=MS.gene93622.t1;Parent=MS.gene93622 |
| chr7.3 | exon | 77735050 | 77735093 | 77735050 | ID=MS.gene93622.t1.exon1;Parent=MS.gene93622.t1 |
| chr7.3 | CDS | 77735050 | 77735093 | 77735050 | ID=cds.MS.gene93622.t1;Parent=MS.gene93622.t1 |
| chr7.3 | exon | 77735361 | 77735463 | 77735361 | ID=MS.gene93622.t1.exon2;Parent=MS.gene93622.t1 |
| chr7.3 | CDS | 77735361 | 77735463 | 77735361 | ID=cds.MS.gene93622.t1;Parent=MS.gene93622.t1 |
| chr7.3 | exon | 77735750 | 77736094 | 77735750 | ID=MS.gene93622.t1.exon3;Parent=MS.gene93622.t1 |
| chr7.3 | CDS | 77735750 | 77736094 | 77735750 | ID=cds.MS.gene93622.t1;Parent=MS.gene93622.t1 |
| Gene Sequence |
| Protein sequence |
