AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93776

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93776.t1 MTR_8g106900 87.589 282 21 4 1 276 1 274 5.29e-152 425
MS.gene93776.t1 MTR_7g104720 56.364 165 66 3 111 275 246 404 1.80e-55 184
MS.gene93776.t1 MTR_1g090400 48.125 160 77 3 116 275 215 368 1.00e-45 157
MS.gene93776.t1 MTR_3g017490 46.452 155 79 2 122 276 111 261 1.11e-42 147
MS.gene93776.t1 MTR_7g106500 43.030 165 87 3 116 275 116 278 9.69e-42 145
MS.gene93776.t1 MTR_1g051180 43.210 162 85 2 120 275 203 363 2.72e-41 146
MS.gene93776.t1 MTR_7g010090 36.071 280 120 8 55 275 152 431 2.37e-38 139
MS.gene93776.t1 MTR_8g038440 46.753 154 75 4 125 275 105 254 4.29e-38 135
MS.gene93776.t1 MTR_7g090230 43.226 155 83 4 122 275 274 424 4.45e-38 139
MS.gene93776.t1 MTR_5g083110 46.452 155 77 3 121 275 90 238 5.24e-37 132
MS.gene93776.t1 MTR_8g099665 35.769 260 114 7 64 275 113 367 1.02e-35 131
MS.gene93776.t1 MTR_6g011640 33.673 294 117 10 60 275 103 396 1.02e-34 129
MS.gene93776.t1 MTR_3g465550 44.667 150 76 3 122 270 84 227 5.45e-34 123
MS.gene93776.t1 MTR_5g072400 46.104 154 77 3 122 275 106 253 1.51e-31 118
MS.gene93776.t1 MTR_3g071510 39.189 148 84 2 128 275 93 234 1.29e-29 112
MS.gene93776.t1 MTR_3g030520 39.490 157 81 3 133 275 142 298 5.18e-25 101
MS.gene93776.t1 MTR_5g048870 37.500 96 57 1 118 210 61 156 2.15e-12 63.9
MS.gene93776.t1 MTR_7g079015 42.045 88 45 2 166 247 1 88 2.74e-11 59.7
MS.gene93776.t1 MTR_1g095960 38.776 98 51 3 170 258 32 129 8.03e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93776.t1 AT4G10630 54.167 336 92 11 1 276 1 334 6.81e-103 303
MS.gene93776.t1 AT1G32760 52.681 317 103 12 2 276 3 314 9.28e-82 249
MS.gene93776.t1 AT5G03870 44.495 218 100 3 61 276 186 384 2.61e-48 165
MS.gene93776.t1 AT1G64500 49.390 164 79 2 116 275 202 365 7.00e-46 158
MS.gene93776.t1 AT5G39865 36.546 249 124 5 61 275 140 388 3.05e-45 157
MS.gene93776.t1 AT5G06470 47.273 165 79 3 116 276 79 239 5.75e-43 147
MS.gene93776.t1 AT5G13810 44.379 169 88 3 108 276 111 273 2.52e-41 144
MS.gene93776.t1 AT5G01420 44.318 176 88 4 105 276 232 401 5.11e-39 140
MS.gene93776.t1 AT5G58530 41.919 198 98 5 90 276 75 266 5.76e-38 135
MS.gene93776.t1 AT3G57070 44.231 156 81 3 122 275 264 415 1.63e-37 137
MS.gene93776.t1 AT2G41330 44.805 154 80 3 123 275 251 400 1.50e-36 134
MS.gene93776.t1 AT3G28850 42.135 178 77 4 124 275 249 426 1.33e-35 132
MS.gene93776.t1 AT3G11773 42.857 147 77 3 130 276 11 150 3.13e-31 114
MS.gene93776.t1 AT3G11773 43.103 116 59 3 161 276 92 200 3.34e-22 91.7

Find 91 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCAAGAGTTAATGATTTC+AGG 0.178045 4.2:-453923 None:intergenic
TGTAGTCATTGTAATGAAAA+TGG 0.191863 4.2:+454523 MS.gene93776:CDS
TCAAAGCAGGGTTTAGGTTT+AGG 0.271658 4.2:-454036 None:intergenic
AAAACCCGGTTCGGCTCTTT+CGG 0.272734 4.2:+454060 MS.gene93776:CDS
TTTGTTGGGAGAGGTTCTTC+AGG 0.290766 4.2:+454366 MS.gene93776:CDS
GTGGAGGAGTGGGTGGATCG+AGG 0.303371 4.2:-453859 None:intergenic
CAGGGTTTAGGTTTAGGTTT+AGG 0.312942 4.2:-454030 None:intergenic
TTTAGGTTTAGGTTTAGGTT+TGG 0.327160 4.2:-454025 None:intergenic
GATGGTGAGTAAACAGAAAC+AGG 0.351897 4.2:+454480 MS.gene93776:CDS
CCGATCCGAAAGAGCCGAAC+CGG 0.370965 4.2:-454065 None:intergenic
TTAGGTTTAGGTTTAGGTTT+GGG 0.393408 4.2:-454024 None:intergenic
ACCACTTTCTTCTCTCCGTT+CGG 0.396019 4.2:-454106 None:intergenic
CACCTGAAATCATTAACTCT+TGG 0.404093 4.2:+453921 MS.gene93776:CDS
AGGTGCCGAACTCGTCTTCA+TGG 0.405385 4.2:-453772 None:intergenic
CGAGAAGGTGGTGGAGGAGT+GGG 0.412430 4.2:-453869 None:intergenic
TCTTTAACTTCCACCACTTA+CGG 0.416969 4.2:+453824 MS.gene93776:CDS
GGTTGTGGAAGAGGGTTTGT+TGG 0.419535 4.2:+454351 MS.gene93776:CDS
TTGTTGGGAGAGGTTCTTCA+GGG 0.419600 4.2:+454367 MS.gene93776:CDS
GTAGTCATTGTAATGAAAAT+GGG 0.424267 4.2:+454524 MS.gene93776:CDS
GTTGTGGAAGAGGGTTTGTT+GGG 0.424770 4.2:+454352 MS.gene93776:CDS
GGAAGAGGGTTTGTTGGGAG+AGG 0.425439 4.2:+454357 MS.gene93776:CDS
GATGTTGAAGGTTGTGGAAG+AGG 0.428644 4.2:+454342 MS.gene93776:CDS
GGGAGAGAAGCGGAAGCTTT+CGG 0.431717 4.2:-453966 None:intergenic
TTCAGGTGGTTCGGAGCGGA+GGG 0.434145 4.2:-453906 None:intergenic
GTTAATGATTTCAGGTGGTT+CGG 0.436171 4.2:-453915 None:intergenic
ACCTGAAATCATTAACTCTT+GGG 0.437778 4.2:+453922 MS.gene93776:CDS
TGGAGGAGTGGGTGGATCGA+GGG 0.448293 4.2:-453858 None:intergenic
ATGTTGAAGGTTGTGGAAGA+GGG 0.449891 4.2:+454343 MS.gene93776:CDS
CATTAACTCTTGGGAACTCA+TGG 0.451980 4.2:+453931 MS.gene93776:CDS
TTTGTGAAGGGATACTATAT+TGG 0.456511 4.2:+454307 MS.gene93776:CDS
GGAGGTGTTTGTGAGGGTTG+CGG 0.462528 4.2:+454412 MS.gene93776:CDS
GTGAAACGAGAAGGTGGTGG+AGG 0.466542 4.2:-453875 None:intergenic
TTTCAGGTGGTTCGGAGCGG+AGG 0.476568 4.2:-453907 None:intergenic
CGTGACGTTTCGATGGATAG+TGG 0.483806 4.2:+454226 MS.gene93776:CDS
CCGGTTCGGCTCTTTCGGAT+CGG 0.506227 4.2:+454065 MS.gene93776:CDS
TTGAACCATGAAGACGAGTT+CGG 0.509241 4.2:+453767 MS.gene93776:CDS
TTGGAAGAAGGGGAGAGAAG+CGG 0.510913 4.2:-453976 None:intergenic
GTTGGTGGAGGTGTTTGTGA+GGG 0.511209 4.2:+454406 MS.gene93776:CDS
CTGCGAGCGTGACGTTTCGA+TGG 0.512099 4.2:+454219 MS.gene93776:CDS
GTTTAGGTTTAGGTTTGGGT+CGG 0.514062 4.2:-454020 None:intergenic
GTGGGTTTAAAGAGGAACTG+AGG 0.517564 4.2:+454245 MS.gene93776:CDS
GATGGATAGTGGGTTTAAAG+AGG 0.524140 4.2:+454237 MS.gene93776:CDS
AGGTGTTCGGAGGACATTTG+AGG 0.530160 4.2:+454150 MS.gene93776:CDS
TGAAGAGATGTTGAAGGTTG+TGG 0.532420 4.2:+454336 MS.gene93776:CDS
CTCTTTCGGATCGGTTTGAG+AGG 0.536689 4.2:+454074 MS.gene93776:CDS
AGAGTGAAACGAGAAGGTGG+TGG 0.542507 4.2:-453878 None:intergenic
GTGAAGGGATACTATATTGG+CGG 0.545463 4.2:+454310 MS.gene93776:CDS
ACGAGAAGGTGGTGGAGGAG+TGG 0.548348 4.2:-453870 None:intergenic
GAAGGTGGTGGAGGAGTGGG+TGG 0.550463 4.2:-453866 None:intergenic
GTGGATCGAGGGTGTTGAGA+AGG 0.552372 4.2:-453847 None:intergenic
TGTTGGTGGAGGTGTTTGTG+AGG 0.554629 4.2:+454405 MS.gene93776:CDS
CAACAACGTTGAGAGGTGTT+CGG 0.556960 4.2:+454137 MS.gene93776:CDS
TTGCCAAGAAAAGCTGTTGG+TGG 0.557146 4.2:+454391 MS.gene93776:CDS
AGCGGAAGCTTTCGGTGTCG+AGG 0.562057 4.2:-453958 None:intergenic
GGCACCTCAGCACTCGGTGT+CGG 0.562950 4.2:+453788 MS.gene93776:CDS
TAGCTAACTAACTACAAAGT+GGG 0.563635 4.2:-454558 None:intergenic
GGTGTTGAGAAGGCCGTAAG+TGG 0.564170 4.2:-453837 None:intergenic
TTGAGAGTGAAACGAGAAGG+TGG 0.565568 4.2:-453881 None:intergenic
CGGTGCTGAAGAGATGTTGA+AGG 0.565844 4.2:+454330 MS.gene93776:CDS
CCGCCGAGAGTGTTTGTGAA+GGG 0.568180 4.2:+454295 MS.gene93776:CDS
ATCTATACAACAACGTTGAG+AGG 0.568839 4.2:+454130 MS.gene93776:CDS
CCAAGAAAAGCTGTTGGTGG+AGG 0.569004 4.2:+454394 MS.gene93776:CDS
ATGTAACGGAAGCTGTAAGA+TGG 0.570898 4.2:+454462 MS.gene93776:CDS
GTGACGTTTCGATGGATAGT+GGG 0.575896 4.2:+454227 MS.gene93776:CDS
ACTTTCTTCTCTCCGTTCGG+CGG 0.582123 4.2:-454103 None:intergenic
GAGTTCGGCACCTCAGCACT+CGG 0.587519 4.2:+453782 MS.gene93776:CDS
TCCGCCGAGAGTGTTTGTGA+AGG 0.587875 4.2:+454294 MS.gene93776:CDS
GTTGAGAAGGCCGTAAGTGG+TGG 0.592261 4.2:-453834 None:intergenic
GAGAGGATATGTCCGCCGAA+CGG 0.594447 4.2:+454091 MS.gene93776:CDS
CGATCCGAAAGAGCCGAACC+GGG 0.602068 4.2:-454064 None:intergenic
CTTCCGTTACATCGAAAACA+AGG 0.602706 4.2:-454451 None:intergenic
GGTTTGAGAGTGAAACGAGA+AGG 0.605117 4.2:-453884 None:intergenic
TCAGGTGGTTCGGAGCGGAG+GGG 0.606340 4.2:-453905 None:intergenic
TATCCCTTCACAAACACTCT+CGG 0.606463 4.2:-454298 None:intergenic
CAAGAGTTAATGATTTCAGG+TGG 0.609204 4.2:-453920 None:intergenic
AACTCTTGGGAACTCATGGA+AGG 0.617874 4.2:+453935 MS.gene93776:CDS
CTAGCTAACTAACTACAAAG+TGG 0.619292 4.2:-454559 None:intergenic
TGGGTTTAAAGAGGAACTGA+GGG 0.619505 4.2:+454246 MS.gene93776:CDS
ATGGTGAGTAAACAGAAACA+GGG 0.621676 4.2:+454481 MS.gene93776:CDS
GGAGCTTCTGAAAGAGAAGA+CGG 0.622205 4.2:+454267 MS.gene93776:CDS
GGTTTGCCAAGAAAAGCTGT+TGG 0.627797 4.2:+454388 MS.gene93776:CDS
TGATTTCAGGTGGTTCGGAG+CGG 0.646701 4.2:-453910 None:intergenic
GCCGAACGGAGAGAAGAAAG+TGG 0.652309 4.2:+454105 MS.gene93776:CDS
AAGTTAAAGAGACGATGTGG+TGG 0.652944 4.2:-453811 None:intergenic
GTGGCCGACACCGAGTGCTG+AGG 0.659050 4.2:-453792 None:intergenic
GACATTTGAGGCTTGCAATG+CGG 0.676497 4.2:+454162 MS.gene93776:CDS
CCCTTCACAAACACTCTCGG+CGG 0.696914 4.2:-454295 None:intergenic
CAACGTTGAGAGGTGTTCGG+AGG 0.699173 4.2:+454140 MS.gene93776:CDS
TGGAAGTTAAAGAGACGATG+TGG 0.732203 4.2:-453814 None:intergenic
TGGTGAGTAAACAGAAACAG+GGG 0.736014 4.2:+454482 MS.gene93776:CDS
GCTTCTGAAAGAGAAGACGG+TGG 0.771527 4.2:+454270 MS.gene93776:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTAGTCATTGTAATGAAAAT+GGG + chr4.2:454524-454543 MS.gene93776:CDS 25.0%
! TGTAGTCATTGTAATGAAAA+TGG + chr4.2:454523-454542 MS.gene93776:CDS 25.0%
ACCTGAAATCATTAACTCTT+GGG + chr4.2:453922-453941 MS.gene93776:CDS 30.0%
TTTGTGAAGGGATACTATAT+TGG + chr4.2:454307-454326 MS.gene93776:CDS 30.0%
! TTAGGTTTAGGTTTAGGTTT+GGG - chr4.2:454027-454046 None:intergenic 30.0%
! TTTAGGTTTAGGTTTAGGTT+TGG - chr4.2:454028-454047 None:intergenic 30.0%
! TTTTCTTTGTTGGATGGTTT+TGG - chr4.2:453998-454017 None:intergenic 30.0%
ATCTATACAACAACGTTGAG+AGG + chr4.2:454130-454149 MS.gene93776:CDS 35.0%
ATGGTGAGTAAACAGAAACA+GGG + chr4.2:454481-454500 MS.gene93776:CDS 35.0%
CAAGAGTTAATGATTTCAGG+TGG - chr4.2:453923-453942 None:intergenic 35.0%
CACCTGAAATCATTAACTCT+TGG + chr4.2:453921-453940 MS.gene93776:CDS 35.0%
TCCCAAGAGTTAATGATTTC+AGG - chr4.2:453926-453945 None:intergenic 35.0%
! GTTAATGATTTCAGGTGGTT+CGG - chr4.2:453918-453937 None:intergenic 35.0%
! TCTTTAACTTCCACCACTTA+CGG + chr4.2:453824-453843 MS.gene93776:CDS 35.0%
!!! GTTTTTTCAAAGCAGGGTTT+AGG - chr4.2:454045-454064 None:intergenic 35.0%
!!! TTGCCTTGTTTTCGATGTAA+CGG + chr4.2:454448-454467 MS.gene93776:CDS 35.0%
AAGTTAAAGAGACGATGTGG+TGG - chr4.2:453814-453833 None:intergenic 40.0%
ATGTTGAAGGTTGTGGAAGA+GGG + chr4.2:454343-454362 MS.gene93776:CDS 40.0%
CATTAACTCTTGGGAACTCA+TGG + chr4.2:453931-453950 MS.gene93776:CDS 40.0%
CTTCCGTTACATCGAAAACA+AGG - chr4.2:454454-454473 None:intergenic 40.0%
GATGGTGAGTAAACAGAAAC+AGG + chr4.2:454480-454499 MS.gene93776:CDS 40.0%
GTGAAGGGATACTATATTGG+CGG + chr4.2:454310-454329 MS.gene93776:CDS 40.0%
TATCCCTTCACAAACACTCT+CGG - chr4.2:454301-454320 None:intergenic 40.0%
TGAAGAGATGTTGAAGGTTG+TGG + chr4.2:454336-454355 MS.gene93776:CDS 40.0%
TGGAAGTTAAAGAGACGATG+TGG - chr4.2:453817-453836 None:intergenic 40.0%
TGGGTTTAAAGAGGAACTGA+GGG + chr4.2:454246-454265 MS.gene93776:CDS 40.0%
TGGTGAGTAAACAGAAACAG+GGG + chr4.2:454482-454501 MS.gene93776:CDS 40.0%
TTGAACCATGAAGACGAGTT+CGG + chr4.2:453767-453786 MS.gene93776:CDS 40.0%
! AGAGCAGCTTTTGATGCATT+TGG + chr4.2:454187-454206 MS.gene93776:CDS 40.0%
! ATGTAACGGAAGCTGTAAGA+TGG + chr4.2:454462-454481 MS.gene93776:CDS 40.0%
! CAGGGTTTAGGTTTAGGTTT+AGG - chr4.2:454033-454052 None:intergenic 40.0%
! GTTTAGGTTTAGGTTTGGGT+CGG - chr4.2:454023-454042 None:intergenic 40.0%
! TCAAAGCAGGGTTTAGGTTT+AGG - chr4.2:454039-454058 None:intergenic 40.0%
!! AACCCTGCTTTGAAAAAACC+CGG + chr4.2:454046-454065 MS.gene93776:CDS 40.0%
!! GATGGATAGTGGGTTTAAAG+AGG + chr4.2:454237-454256 MS.gene93776:CDS 40.0%
!! TCGGTGTTTTCTTTGTTGGA+TGG - chr4.2:454004-454023 None:intergenic 40.0%
!! TGCTTTGAAAAAACCCGGTT+CGG + chr4.2:454051-454070 MS.gene93776:CDS 40.0%
!!! AACCGGGTTTTTTCAAAGCA+GGG - chr4.2:454051-454070 None:intergenic 40.0%
!!! GTTGGATGGTTTTGGAAGAA+GGG - chr4.2:453990-454009 None:intergenic 40.0%
!!! TGTTGGATGGTTTTGGAAGA+AGG - chr4.2:453991-454010 None:intergenic 40.0%
!!! TTGGATGGTTTTGGAAGAAG+GGG - chr4.2:453989-454008 None:intergenic 40.0%
AACTCTTGGGAACTCATGGA+AGG + chr4.2:453935-453954 MS.gene93776:CDS 45.0%
ACCACTTTCTTCTCTCCGTT+CGG - chr4.2:454109-454128 None:intergenic 45.0%
GATGTTGAAGGTTGTGGAAG+AGG + chr4.2:454342-454361 MS.gene93776:CDS 45.0%
GGTTTGAGAGTGAAACGAGA+AGG - chr4.2:453887-453906 None:intergenic 45.0%
GGTTTGCCAAGAAAAGCTGT+TGG + chr4.2:454388-454407 MS.gene93776:CDS 45.0%
GTGACGTTTCGATGGATAGT+GGG + chr4.2:454227-454246 MS.gene93776:CDS 45.0%
GTGGGTTTAAAGAGGAACTG+AGG + chr4.2:454245-454264 MS.gene93776:CDS 45.0%
TTGAGAGTGAAACGAGAAGG+TGG - chr4.2:453884-453903 None:intergenic 45.0%
TTGCCAAGAAAAGCTGTTGG+TGG + chr4.2:454391-454410 MS.gene93776:CDS 45.0%
TTGTTGGGAGAGGTTCTTCA+GGG + chr4.2:454367-454386 MS.gene93776:CDS 45.0%
TTTGTTGGGAGAGGTTCTTC+AGG + chr4.2:454366-454385 MS.gene93776:CDS 45.0%
! GACATTTGAGGCTTGCAATG+CGG + chr4.2:454162-454181 MS.gene93776:CDS 45.0%
! GGAGCTTCTGAAAGAGAAGA+CGG + chr4.2:454267-454286 MS.gene93776:CDS 45.0%
! GTTGTGGAAGAGGGTTTGTT+GGG + chr4.2:454352-454371 MS.gene93776:CDS 45.0%
!! CAACAACGTTGAGAGGTGTT+CGG + chr4.2:454137-454156 MS.gene93776:CDS 45.0%
!! TGGGTCGGTGTTTTCTTTGT+TGG - chr4.2:454008-454027 None:intergenic 45.0%
!!! GAACCGGGTTTTTTCAAAGC+AGG - chr4.2:454052-454071 None:intergenic 45.0%
ACTTTCTTCTCTCCGTTCGG+CGG - chr4.2:454106-454125 None:intergenic 50.0%
AGAGTGAAACGAGAAGGTGG+TGG - chr4.2:453881-453900 None:intergenic 50.0%
AGGTGTTCGGAGGACATTTG+AGG + chr4.2:454150-454169 MS.gene93776:CDS 50.0%
CGTGACGTTTCGATGGATAG+TGG + chr4.2:454226-454245 MS.gene93776:CDS 50.0%
GGTTGTGGAAGAGGGTTTGT+TGG + chr4.2:454351-454370 MS.gene93776:CDS 50.0%
TTGGAAGAAGGGGAGAGAAG+CGG - chr4.2:453979-453998 None:intergenic 50.0%
! CCAAGAAAAGCTGTTGGTGG+AGG + chr4.2:454394-454413 MS.gene93776:CDS 50.0%
! CCTCCACCAACAGCTTTTCT+TGG - chr4.2:454397-454416 None:intergenic 50.0%
! CGGTGCTGAAGAGATGTTGA+AGG + chr4.2:454330-454349 MS.gene93776:CDS 50.0%
! GCTTCTGAAAGAGAAGACGG+TGG + chr4.2:454270-454289 MS.gene93776:CDS 50.0%
! TGATTTCAGGTGGTTCGGAG+CGG - chr4.2:453913-453932 None:intergenic 50.0%
!! AAAACCCGGTTCGGCTCTTT+CGG + chr4.2:454060-454079 MS.gene93776:CDS 50.0%
!! CTCTTTCGGATCGGTTTGAG+AGG + chr4.2:454074-454093 MS.gene93776:CDS 50.0%
!! GTTGGTGGAGGTGTTTGTGA+GGG + chr4.2:454406-454425 MS.gene93776:CDS 50.0%
!! TGTTGGTGGAGGTGTTTGTG+AGG + chr4.2:454405-454424 MS.gene93776:CDS 50.0%
CCCTTCACAAACACTCTCGG+CGG - chr4.2:454298-454317 None:intergenic 55.0%
GAGAGGATATGTCCGCCGAA+CGG + chr4.2:454091-454110 MS.gene93776:CDS 55.0%
GCCGAACGGAGAGAAGAAAG+TGG + chr4.2:454105-454124 MS.gene93776:CDS 55.0%
GGGAGAGAAGCGGAAGCTTT+CGG - chr4.2:453969-453988 None:intergenic 55.0%
GTTGAGAAGGCCGTAAGTGG+TGG - chr4.2:453837-453856 None:intergenic 55.0%
! GGTGTTGAGAAGGCCGTAAG+TGG - chr4.2:453840-453859 None:intergenic 55.0%
! GTGAAACGAGAAGGTGGTGG+AGG - chr4.2:453878-453897 None:intergenic 55.0%
!! AGGTGCCGAACTCGTCTTCA+TGG - chr4.2:453775-453794 None:intergenic 55.0%
!! CAACGTTGAGAGGTGTTCGG+AGG + chr4.2:454140-454159 MS.gene93776:CDS 55.0%
!! CCGCCGAGAGTGTTTGTGAA+GGG + chr4.2:454295-454314 MS.gene93776:CDS 55.0%
!! GGAAGAGGGTTTGTTGGGAG+AGG + chr4.2:454357-454376 MS.gene93776:CDS 55.0%
!! GGAGGTGTTTGTGAGGGTTG+CGG + chr4.2:454412-454431 MS.gene93776:CDS 55.0%
!! GTGGATCGAGGGTGTTGAGA+AGG - chr4.2:453850-453869 None:intergenic 55.0%
!! TCCGCCGAGAGTGTTTGTGA+AGG + chr4.2:454294-454313 MS.gene93776:CDS 55.0%
CCGATCCGAAAGAGCCGAAC+CGG - chr4.2:454068-454087 None:intergenic 60.0%
CGATCCGAAAGAGCCGAACC+GGG - chr4.2:454067-454086 None:intergenic 60.0%
CTGCGAGCGTGACGTTTCGA+TGG + chr4.2:454219-454238 MS.gene93776:CDS 60.0%
GAGTTCGGCACCTCAGCACT+CGG + chr4.2:453782-453801 MS.gene93776:CDS 60.0%
TGGAGGAGTGGGTGGATCGA+GGG - chr4.2:453861-453880 None:intergenic 60.0%
! TTCAGGTGGTTCGGAGCGGA+GGG - chr4.2:453909-453928 None:intergenic 60.0%
! TTTCAGGTGGTTCGGAGCGG+AGG - chr4.2:453910-453929 None:intergenic 60.0%
!! ACGAGAAGGTGGTGGAGGAG+TGG - chr4.2:453873-453892 None:intergenic 60.0%
!! AGCGGAAGCTTTCGGTGTCG+AGG - chr4.2:453961-453980 None:intergenic 60.0%
!! CCGGTTCGGCTCTTTCGGAT+CGG + chr4.2:454065-454084 MS.gene93776:CDS 60.0%
!! CGAGAAGGTGGTGGAGGAGT+GGG - chr4.2:453872-453891 None:intergenic 60.0%
GTGGAGGAGTGGGTGGATCG+AGG - chr4.2:453862-453881 None:intergenic 65.0%
! TCAGGTGGTTCGGAGCGGAG+GGG - chr4.2:453908-453927 None:intergenic 65.0%
!! GAAGGTGGTGGAGGAGTGGG+TGG - chr4.2:453869-453888 None:intergenic 65.0%
!! GGCACCTCAGCACTCGGTGT+CGG + chr4.2:453788-453807 MS.gene93776:CDS 65.0%
! GTGGCCGACACCGAGTGCTG+AGG - chr4.2:453795-453814 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 453743 454573 453743 ID=MS.gene93776
chr4.2 mRNA 453743 454573 453743 ID=MS.gene93776.t1;Parent=MS.gene93776
chr4.2 exon 453743 454573 453743 ID=MS.gene93776.t1.exon1;Parent=MS.gene93776.t1
chr4.2 CDS 453743 454573 453743 ID=cds.MS.gene93776.t1;Parent=MS.gene93776.t1
Gene Sequence

>MS.gene93776

ATGGGTTGCGTGTCTTCACATCTGTTGAACCATGAAGACGAGTTCGGCACCTCAGCACTCGGTGTCGGCCACCACATCGTCTCTTTAACTTCCACCACTTACGGCCTTCTCAACACCCTCGATCCACCCACTCCTCCACCACCTTCTCGTTTCACTCTCAAACCCCTCCGCTCCGAACCACCTGAAATCATTAACTCTTGGGAACTCATGGAAGGCCTCGACACCGAAAGCTTCCGCTTCTCTCCCCTTCTTCCAAAACCATCCAACAAAGAAAACACCGACCCAAACCTAAACCTAAACCTAAACCCTGCTTTGAAAAAACCCGGTTCGGCTCTTTCGGATCGGTTTGAGAGGATATGTCCGCCGAACGGAGAGAAGAAAGTGGTGATCTATACAACAACGTTGAGAGGTGTTCGGAGGACATTTGAGGCTTGCAATGCGGTGAGAGCAGCTTTTGATGCATTTGGTGTACAAATCTGCGAGCGTGACGTTTCGATGGATAGTGGGTTTAAAGAGGAACTGAGGGAGCTTCTGAAAGAGAAGACGGTGGTTCCGCCGAGAGTGTTTGTGAAGGGATACTATATTGGCGGTGCTGAAGAGATGTTGAAGGTTGTGGAAGAGGGTTTGTTGGGAGAGGTTCTTCAGGGTTTGCCAAGAAAAGCTGTTGGTGGAGGTGTTTGTGAGGGTTGCGGTGATATGAGATTCTTGCCTTGTTTTCGATGTAACGGAAGCTGTAAGATGGTGAGTAAACAGAAACAGGGGAACACTGTTGTTGTTAAGTGTAGTCATTGTAATGAAAATGGGTTAGTTCTGTGCCCACTTTGTAGTTAG
Protein sequence

>MS.gene93776.t1

MGCVSSHLLNHEDEFGTSALGVGHHIVSLTSTTYGLLNTLDPPTPPPPSRFTLKPLRSEPPEIINSWELMEGLDTESFRFSPLLPKPSNKENTDPNLNLNLNPALKKPGSALSDRFERICPPNGEKKVVIYTTTLRGVRRTFEACNAVRAAFDAFGVQICERDVSMDSGFKEELRELLKEKTVVPPRVFVKGYYIGGAEEMLKVVEEGLLGEVLQGLPRKAVGGGVCEGCGDMRFLPCFRCNGSCKMVSKQKQGNTVVVKCSHCNENGLVLCPLCS