AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93785

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93785.t1 MTR_8g106740 95.785 261 7 1 1 257 1 261 0.0 509
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93785.t1 AT3G18165 63.265 245 87 2 8 249 2 246 1.48e-113 327

Find 58 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAATTATTACCTGAAAAT+TGG 0.178579 4.1:-693360 None:intergenic
AATATACAGAATGGAAAATT+TGG 0.215246 4.1:+693711 MS.gene93785:intron
TTGGAGTTGTTGGTTAAATA+TGG 0.222898 4.1:+693730 MS.gene93785:CDS
AACCACCACCTGTTAATAAA+TGG 0.267827 4.1:+693530 MS.gene93785:CDS
TCATCGTTCCATTTATTAAC+AGG 0.301941 4.1:-693538 None:intergenic
TTGGATGCTATCACTGAAAA+TGG 0.329024 4.1:+696434 MS.gene93785:CDS
AATGGAAAATTTGGAGTTGT+TGG 0.333732 4.1:+693720 MS.gene93785:CDS
CCTGATGATATTTCCTTTCA+CGG 0.355650 4.1:-696090 None:intergenic
AAATGGCCAACTGGCAAATT+CGG 0.358938 4.1:+696451 MS.gene93785:CDS
TTGTGCTGTTTCCATGCATC+AGG 0.378418 4.1:-693754 None:intergenic
GGCCTCCTGTTTCTCTTGAT+AGG 0.382975 4.1:+693491 MS.gene93785:CDS
TGGTGGTTCTAATTCATATC+TGG 0.391099 4.1:-693515 None:intergenic
CTTCAGGATTATCCAATGAT+TGG 0.394401 4.1:+693439 MS.gene93785:intron
CTGTAATCGTCGTCGATGTA+AGG 0.408083 4.1:-693230 None:intergenic
AGGCAATGCATCGATGATTT+CGG 0.412030 4.1:-693210 None:intergenic
CTGGACCTATCAAGAGAAAC+AGG 0.424750 4.1:-693496 None:intergenic
CTTGAAGCTCCACCTTCTTA+CGG 0.442648 4.1:+693167 MS.gene93785:CDS
TCTTTCCAGAGGCTGGCACT+TGG 0.445834 4.1:+696415 MS.gene93785:intron
TCGACTTCGATCTTAACCCT+TGG 0.460976 4.1:-693257 None:intergenic
CGACTTCGATCTTAACCCTT+GGG 0.465911 4.1:-693256 None:intergenic
CAGAATGCAGAAATTAGCTC+AGG 0.472152 4.1:+696039 MS.gene93785:intron
TTCCATTTATTAACAGGTGG+TGG 0.485498 4.1:-693532 None:intergenic
TCATACTCTCTACCAATCAT+TGG 0.493778 4.1:-693451 None:intergenic
TTCTTCAATATCAAACCATC+AGG 0.495320 4.1:+693599 MS.gene93785:CDS
TATCACTGAAAATGGCCAAC+TGG 0.495702 4.1:+696442 MS.gene93785:CDS
CCGTGAAAGGAAATATCATC+AGG 0.513849 4.1:+696090 MS.gene93785:CDS
GAAGAGGTGGAAAATCTTTG+AGG 0.518940 4.1:-693331 None:intergenic
GGAAGAAGCAGCAGCAGTAA+CGG 0.525910 4.1:+693131 MS.gene93785:CDS
GAGTATGAGCGTGTAAGAGC+TGG 0.533871 4.1:+693466 MS.gene93785:CDS
AATGGAACGATGAAACTGCT+TGG 0.535567 4.1:+693548 MS.gene93785:CDS
TGCATCGATGATTTCGGCGT+CGG 0.537750 4.1:-693204 None:intergenic
CGATGATTTCGGCGTCGGAA+TGG 0.540962 4.1:-693199 None:intergenic
TTAAATATGGCCCTGATGCA+TGG 0.542409 4.1:+693743 MS.gene93785:CDS
TGAAAATTGGAAGAGGGAAG+AGG 0.551095 4.1:-693347 None:intergenic
ACAGAGCTCATTCCATTGCA+TGG 0.563696 4.1:-696238 None:intergenic
TATTACCTGAAAATTGGAAG+AGG 0.565356 4.1:-693354 None:intergenic
ATGATTCCGAATTTGCCAGT+TGG 0.571957 4.1:-696457 None:intergenic
TGAGAAGAGAAGCAGCAGAA+AGG 0.572177 4.1:+696319 MS.gene93785:CDS
ATTACCTGAAAATTGGAAGA+GGG 0.574966 4.1:-693353 None:intergenic
ACGAAAGGGTAAATCATGAT+GGG 0.575853 4.1:+693104 None:intergenic
GGGTAAATCATGATGGGAGA+AGG 0.576400 4.1:+693110 None:intergenic
AAGCTCCACCTTCTTACGGT+AGG 0.576815 4.1:+693171 MS.gene93785:CDS
GAAACAGCACAATCAACGAT+TGG 0.581574 4.1:+693765 MS.gene93785:CDS
GATTGGAAGTATACTTGTCA+AGG 0.585048 4.1:+693782 MS.gene93785:CDS
TGGCTAGACCTACCGTAAGA+AGG 0.586301 4.1:-693179 None:intergenic
TCGTTCCATTTATTAACAGG+TGG 0.588971 4.1:-693535 None:intergenic
AAATCTTTGAGGAAATCAGC+GGG 0.596837 4.1:-693320 None:intergenic
TTAATGCTTTGTCCATGCAA+TGG 0.598699 4.1:+696226 MS.gene93785:CDS
AAATTGGAAGAGGGAAGAGG+TGG 0.604837 4.1:-693344 None:intergenic
ACGACGATTACAGCGACCCA+AGG 0.617860 4.1:+693240 MS.gene93785:CDS
TGTGCTGTTTCCATGCATCA+GGG 0.618858 4.1:-693753 None:intergenic
CTAGACCTACCGTAAGAAGG+TGG 0.632917 4.1:-693176 None:intergenic
AAAATCTTTGAGGAAATCAG+CGG 0.660874 4.1:-693321 None:intergenic
GCATCTCTTCTTCAACGAGA+CGG 0.670836 4.1:-693280 None:intergenic
CAAAGCATTAAGCTCATATG+CGG 0.683946 4.1:-696214 None:intergenic
CGACGATTACAGCGACCCAA+GGG 0.693830 4.1:+693241 MS.gene93785:CDS
ATGAGCGTGTAAGAGCTGGT+AGG 0.696204 4.1:+693470 MS.gene93785:CDS
GACCTATCAAGAGAAACAGG+AGG 0.712656 4.1:-693493 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAACTTAAAAGAAAA+AGG - chr4.1:694422-694441 None:intergenic 10.0%
!!! ATTCTATTTTTTAATGAATA+AGG + chr4.1:695281-695300 MS.gene93785:intron 10.0%
!!! TTTAATTCTTGTTTTTTTTT+TGG + chr4.1:694884-694903 MS.gene93785:intron 10.0%
!! ATTAAAAACTTAGAAGAAAA+AGG - chr4.1:695203-695222 None:intergenic 15.0%
!! GAAAATGAAAATGAAAAATA+TGG - chr4.1:694270-694289 None:intergenic 15.0%
!! AATATACAGAATGGAAAATT+TGG + chr4.1:693711-693730 MS.gene93785:intron 20.0%
!! AGAACATATTTAATGAATCA+AGG - chr4.1:694210-694229 None:intergenic 20.0%
!! AGAATGTTTCATAGTAAATA+TGG + chr4.1:694029-694048 MS.gene93785:intron 20.0%
!! ATATTTAATGAATCAAGGTT+GGG - chr4.1:694205-694224 None:intergenic 20.0%
!! ATGAATGTTTCACATAAATA+TGG + chr4.1:695454-695473 MS.gene93785:intron 20.0%
!! ATTGATTACTTTCATGTTTA+GGG + chr4.1:694374-694393 MS.gene93785:intron 20.0%
!! CAAGAAAAACAAAATTTACA+AGG - chr4.1:696353-696372 None:intergenic 20.0%
!! GTATATTAACTTAAACTAGT+TGG - chr4.1:693698-693717 None:intergenic 20.0%
!! GTTAATTATTACCTGAAAAT+TGG - chr4.1:693363-693382 None:intergenic 20.0%
!! TTAAAATCGATGAAAGAAAA+AGG - chr4.1:695941-695960 None:intergenic 20.0%
!! TTTAAAAATAACAGTGACAA+GGG - chr4.1:696137-696156 None:intergenic 20.0%
!!! AATTCTTCATATTTTTCTCT+TGG + chr4.1:695356-695375 MS.gene93785:intron 20.0%
!!! CTTGTAAATTTTGTTTTTCT+TGG + chr4.1:696351-696370 MS.gene93785:intron 20.0%
!!! GTTTAAGTTAATATACAGAA+TGG + chr4.1:693702-693721 MS.gene93785:intron 20.0%
!!! TTTCATCGATTTTAATTTTG+AGG + chr4.1:695946-695965 MS.gene93785:intron 20.0%
!!! TTTTAAAAATAACAGTGACA+AGG - chr4.1:696138-696157 None:intergenic 20.0%
!!! TTTTTCCTTTTTTCAAAACA+AGG + chr4.1:695222-695241 MS.gene93785:intron 20.0%
! AAGAAAAAGGTGAATAAAAC+TGG - chr4.1:694409-694428 None:intergenic 25.0%
! AGAACACATTTAATGAATCA+AGG - chr4.1:694990-695009 None:intergenic 25.0%
! ATCTACTACCAATAAGTAAA+TGG - chr4.1:694356-694375 None:intergenic 25.0%
! ATTAAATATGTTCTTGACTC+TGG + chr4.1:694216-694235 MS.gene93785:intron 25.0%
! CATATTTAATGAATCAAGGT+TGG - chr4.1:694206-694225 None:intergenic 25.0%
! CTTGTTAATTAGGAACTTAA+GGG - chr4.1:695520-695539 None:intergenic 25.0%
! GAAAAGGAAACTGAAAAATA+TGG - chr4.1:695050-695069 None:intergenic 25.0%
! GATTGATTACTTTCATGTTT+AGG + chr4.1:694373-694392 MS.gene93785:intron 25.0%
! TAAAAAAATAGAATGACCCA+TGG - chr4.1:694491-694510 None:intergenic 25.0%
! TAATATACCTCTTGTTGAAT+TGG + chr4.1:693982-694001 MS.gene93785:intron 25.0%
!! AATTTTCCCTTTTCAAAACA+AGG + chr4.1:694439-694458 MS.gene93785:intron 25.0%
!! ACTTGTTAATTAGGAACTTA+AGG - chr4.1:695521-695540 None:intergenic 25.0%
!! GGAAGAAATTTAAAACAGAA+AGG - chr4.1:694743-694762 None:intergenic 25.0%
!! GGTTTGAATACTTGTTAATT+AGG - chr4.1:695530-695549 None:intergenic 25.0%
!! TTTATTCTTCCATATTGGTT+TGG + chr4.1:695554-695573 MS.gene93785:intron 25.0%
!!! ACTTTGTGTTTTGTTTGAAA+AGG - chr4.1:695066-695085 None:intergenic 25.0%
!!! TCTCAAAATTACTAGTCTTA+AGG + chr4.1:695893-695912 MS.gene93785:intron 25.0%
!!! TTCAACCTTGTTTTGAAAAA+AGG - chr4.1:695230-695249 None:intergenic 25.0%
!!! TTTTAAGACCTTATTAGCTA+CGG + chr4.1:694908-694927 MS.gene93785:intron 25.0%
AAATCAAAACAAAACCCTAG+CGG - chr4.1:693410-693429 None:intergenic 30.0%
AAATTAAAGTGAAGACCTGA+TGG - chr4.1:693617-693636 None:intergenic 30.0%
AATATGAGCCACAGTATAAT+TGG - chr4.1:693821-693840 None:intergenic 30.0%
ACAAAGTATGTTCCTTTAAC+TGG + chr4.1:695079-695098 MS.gene93785:intron 30.0%
ATTAAATGTGTTCTTGACTC+TGG + chr4.1:694996-695015 MS.gene93785:intron 30.0%
ATTACCTGAAAATTGGAAGA+GGG - chr4.1:693356-693375 None:intergenic 30.0%
ATTTCTTCCCAAAAAGCAAT+TGG + chr4.1:694754-694773 MS.gene93785:intron 30.0%
CAATATGGAAGAATAAAGTC+AGG - chr4.1:695551-695570 None:intergenic 30.0%
CTGACTTTATTCTTCCATAT+TGG + chr4.1:695549-695568 MS.gene93785:intron 30.0%
GGAAAAGAAGAAATCTCTTA+AGG + chr4.1:694159-694178 MS.gene93785:intron 30.0%
GGAAACTGAAAAATATGGAT+CGG - chr4.1:695045-695064 None:intergenic 30.0%
TAACTGGCAATGATGATAAA+AGG + chr4.1:694315-694334 MS.gene93785:intron 30.0%
TACTTCTATGTCTTCTATCA+TGG + chr4.1:695255-695274 MS.gene93785:intron 30.0%
TATTACCTGAAAATTGGAAG+AGG - chr4.1:693357-693376 None:intergenic 30.0%
TCACATAAATATGGTAGTTG+AGG + chr4.1:695463-695482 MS.gene93785:intron 30.0%
TCATCGTTCCATTTATTAAC+AGG - chr4.1:693541-693560 None:intergenic 30.0%
TCTACTAGCATAGAAGTAAA+TGG - chr4.1:695136-695155 None:intergenic 30.0%
TGATTCAGGAGAAAAATAGA+AGG - chr4.1:694533-694552 None:intergenic 30.0%
TTATCATCATTGCCAGTTAA+AGG - chr4.1:694314-694333 None:intergenic 30.0%
TTCTTCAATATCAAACCATC+AGG + chr4.1:693599-693618 MS.gene93785:CDS 30.0%
TTGAAAAAGTTAACCGTGAA+AGG + chr4.1:696077-696096 MS.gene93785:CDS 30.0%
TTGTTCATCCAATTATACTG+TGG + chr4.1:693810-693829 MS.gene93785:intron 30.0%
! AAAATCTTTGAGGAAATCAG+CGG - chr4.1:693324-693343 None:intergenic 30.0%
! AATGGAAAATTTGGAGTTGT+TGG + chr4.1:693720-693739 MS.gene93785:CDS 30.0%
! ACAAGAGGTATATTAATCTG+AGG - chr4.1:693977-693996 None:intergenic 30.0%
! CTCCTTTTTCTTCACATAAA+AGG + chr4.1:695836-695855 MS.gene93785:intron 30.0%
! TATCTCTTTTCGTATAGCAT+TGG + chr4.1:695994-696013 MS.gene93785:intron 30.0%
! TATTTTTCTCCTGAATCCAA+AGG + chr4.1:695319-695338 MS.gene93785:intron 30.0%
! TTCTTCTTTTCCAGCTAATA+AGG - chr4.1:694151-694170 None:intergenic 30.0%
! TTGGAGTTGTTGGTTAAATA+TGG + chr4.1:693730-693749 MS.gene93785:CDS 30.0%
!! AATTTGATTTGCACAAGTAC+AGG - chr4.1:693909-693928 None:intergenic 30.0%
!! ACTAGTAATTTTGAGAGCAT+TGG - chr4.1:695889-695908 None:intergenic 30.0%
!! TCTATTTTTCTCCTGAATCA+AGG + chr4.1:694533-694552 MS.gene93785:intron 30.0%
!!! ACTTCAACCTTGTTTTGAAA+AGG - chr4.1:694449-694468 None:intergenic 30.0%
!!! AGTTTTAGAGTGTTAGAAAG+AGG + chr4.1:695754-695773 MS.gene93785:intron 30.0%
!!! CTAGTAATTTTGAGAGCATT+GGG - chr4.1:695888-695907 None:intergenic 30.0%
!!! CTTCAACCTTGTTTTGAAAA+GGG - chr4.1:694448-694467 None:intergenic 30.0%
!!! GTTTTAGAGTGTTAGAAAGA+GGG + chr4.1:695755-695774 MS.gene93785:intron 30.0%
AAAAATAACAGTGACAAGGG+TGG - chr4.1:696134-696153 None:intergenic 35.0%
AAAATGAGATCAGTTCACTG+TGG + chr4.1:695394-695413 MS.gene93785:intron 35.0%
AACCACCACCTGTTAATAAA+TGG + chr4.1:693530-693549 MS.gene93785:CDS 35.0%
AATCAAAACAAAACCCTAGC+GGG - chr4.1:693409-693428 None:intergenic 35.0%
ACAAAGTCTGTTCCTTTAAC+TGG + chr4.1:694299-694318 MS.gene93785:intron 35.0%
ACATCTTTGTCTTCAACCAT+GGG + chr4.1:694472-694491 MS.gene93785:intron 35.0%
AGTTGCTTAGACTGCAATTA+GGG - chr4.1:695818-695837 None:intergenic 35.0%
CCTGATGATATTTCCTTTCA+CGG - chr4.1:696093-696112 None:intergenic 35.0%
CTAAAACTCACTCTCCTATT+GGG - chr4.1:695742-695761 None:intergenic 35.0%
CTTCAGGATTATCCAATGAT+TGG + chr4.1:693439-693458 MS.gene93785:intron 35.0%
GATATTGCATACAACAACCA+AGG - chr4.1:694072-694091 None:intergenic 35.0%
GATTCTTTAAGTCTCTTGCT+TGG + chr4.1:695625-695644 MS.gene93785:intron 35.0%
GGAATGTTTCACAGCAAATA+TGG + chr4.1:694792-694811 MS.gene93785:intron 35.0%
GGATTCAGGAGAAAAATAGA+AGG - chr4.1:695317-695336 None:intergenic 35.0%
TAAGTAAGCAAATGATGCAC+AGG - chr4.1:694646-694665 None:intergenic 35.0%
TACATCTTTGTCTTCAACCA+TGG + chr4.1:694471-694490 MS.gene93785:intron 35.0%
TCAAGAACCAATTCAACAAG+AGG - chr4.1:693992-694011 None:intergenic 35.0%
TCATACTCTCTACCAATCAT+TGG - chr4.1:693454-693473 None:intergenic 35.0%
TCGTTCCATTTATTAACAGG+TGG - chr4.1:693538-693557 None:intergenic 35.0%
TTAATGCTTTGTCCATGCAA+TGG + chr4.1:696226-696245 MS.gene93785:CDS 35.0%
TTCCATTTATTAACAGGTGG+TGG - chr4.1:693535-693554 None:intergenic 35.0%
TTCTTCTTCCGTAGCTAATA+AGG - chr4.1:694919-694938 None:intergenic 35.0%
TTGATTTACGATTCTGCTTC+AGG + chr4.1:693423-693442 MS.gene93785:intron 35.0%
TTGGATGCTATCACTGAAAA+TGG + chr4.1:696434-696453 MS.gene93785:CDS 35.0%
TTGTTGTATGCAATATCCTG+CGG + chr4.1:694075-694094 MS.gene93785:intron 35.0%
TTTCAACCATGATTGACCTT+TGG - chr4.1:695338-695357 None:intergenic 35.0%
! AAATCTTTGAGGAAATCAGC+GGG - chr4.1:693323-693342 None:intergenic 35.0%
! CAAAGCATTAAGCTCATATG+CGG - chr4.1:696217-696236 None:intergenic 35.0%
! CGCCTTTTATGTGAAGAAAA+AGG - chr4.1:695841-695860 None:intergenic 35.0%
! GATTGGAAGTATACTTGTCA+AGG + chr4.1:693782-693801 MS.gene93785:CDS 35.0%
! TCTAAAACTCACTCTCCTAT+TGG - chr4.1:695743-695762 None:intergenic 35.0%
! TTGCGTGCATTTTATGTACA+AGG - chr4.1:695687-695706 None:intergenic 35.0%
!! GACTTTAAGACCTTATTAGC+TGG + chr4.1:694138-694157 MS.gene93785:intron 35.0%
!! TGGCTGATTTCATCTAGTTT+TGG + chr4.1:696371-696390 MS.gene93785:intron 35.0%
!! TGGTGGTTCTAATTCATATC+TGG - chr4.1:693518-693537 None:intergenic 35.0%
!!! GCTTAAGCCAATTGCTTTTT+GGG - chr4.1:694764-694783 None:intergenic 35.0%
!!! GTTTTTTTCTCTCTTTCCAG+AGG + chr4.1:696404-696423 MS.gene93785:intron 35.0%
AAAATGAGATCAGTGCTCTG+CGG + chr4.1:694606-694625 MS.gene93785:intron 40.0%
AAATGGCCAACTGGCAAATT+CGG + chr4.1:696451-696470 MS.gene93785:CDS 40.0%
AATCCACACAGCAACAAATG+TGG - chr4.1:693863-693882 None:intergenic 40.0%
AATGGAACGATGAAACTGCT+TGG + chr4.1:693548-693567 MS.gene93785:CDS 40.0%
ACACATGAGTTTCACCCAAT+AGG + chr4.1:695725-695744 MS.gene93785:intron 40.0%
ACGGAAGAAGAAATCTCTTG+AGG + chr4.1:694927-694946 MS.gene93785:intron 40.0%
ATATGTCTCGAGCACATCAA+AGG - chr4.1:695496-695515 None:intergenic 40.0%
ATGATAAAAGGCCGGTCTTA+CGG + chr4.1:694327-694346 MS.gene93785:intron 40.0%
ATGATTCCGAATTTGCCAGT+TGG - chr4.1:696460-696479 None:intergenic 40.0%
CAGAATGCAGAAATTAGCTC+AGG + chr4.1:696039-696058 MS.gene93785:intron 40.0%
CAGTCATACAGAACAAATGC+AGG - chr4.1:694697-694716 None:intergenic 40.0%
CATGATTGACCTTTGGATTC+AGG - chr4.1:695331-695350 None:intergenic 40.0%
CCGTGAAAGGAAATATCATC+AGG + chr4.1:696090-696109 MS.gene93785:CDS 40.0%
CTCAAGCAACCAAACCAATA+TGG - chr4.1:695566-695585 None:intergenic 40.0%
CTGAATCCAAAGGTCAATCA+TGG + chr4.1:695329-695348 MS.gene93785:intron 40.0%
GAAACAGCACAATCAACGAT+TGG + chr4.1:693765-693784 MS.gene93785:CDS 40.0%
GAAGAGGTGGAAAATCTTTG+AGG - chr4.1:693334-693353 None:intergenic 40.0%
GAGTTGCTTAGACTGCAATT+AGG - chr4.1:695819-695838 None:intergenic 40.0%
GGAGAAAGAGTTAGTTAGTG+TGG - chr4.1:693385-693404 None:intergenic 40.0%
GTATTCCTGGCATTTCTCTA+GGG + chr4.1:695777-695796 MS.gene93785:intron 40.0%
GTTGCATACAACAACAACCA+AGG - chr4.1:694834-694853 None:intergenic 40.0%
TATCACTGAAAATGGCCAAC+TGG + chr4.1:696442-696461 MS.gene93785:CDS 40.0%
TATTCCTGGCATTTCTCTAG+GGG + chr4.1:695778-695797 MS.gene93785:intron 40.0%
TGAAAATTGGAAGAGGGAAG+AGG - chr4.1:693350-693369 None:intergenic 40.0%
TGGCAATGATGATAAAAGGC+CGG + chr4.1:694319-694338 MS.gene93785:intron 40.0%
TGGGTGAAACTCATGTGTTA+AGG - chr4.1:695723-695742 None:intergenic 40.0%
TGTGAAACATTCATATCCGC+AGG - chr4.1:695448-695467 None:intergenic 40.0%
TGTTGTATGCAATATCCTGC+TGG + chr4.1:694076-694095 MS.gene93785:intron 40.0%
TTAAATATGGCCCTGATGCA+TGG + chr4.1:693743-693762 MS.gene93785:CDS 40.0%
! AAAGCAATTGGCTTAAGCCT+CGG + chr4.1:694766-694785 MS.gene93785:intron 40.0%
! AGGCAATGCATCGATGATTT+CGG - chr4.1:693213-693232 None:intergenic 40.0%
! ATTCCACATTTGTTGCTGTG+TGG + chr4.1:693857-693876 MS.gene93785:intron 40.0%
! TACGGCTGCCATTTACTTAT+TGG + chr4.1:694345-694364 MS.gene93785:intron 40.0%
! TCTTCCCTCTTCCAATTTTC+AGG + chr4.1:693349-693368 MS.gene93785:CDS 40.0%
! TTGTTGTTGTATGCAACTGC+TGG + chr4.1:694837-694856 MS.gene93785:intron 40.0%
!! GGCTTAAGCCAATTGCTTTT+TGG - chr4.1:694765-694784 None:intergenic 40.0%
!! TGTTAGAAAGAGGGTATTCC+TGG + chr4.1:695764-695783 MS.gene93785:intron 40.0%
AAATTGGAAGAGGGAAGAGG+TGG - chr4.1:693347-693366 None:intergenic 45.0%
ACAGAGCTCATTCCATTGCA+TGG - chr4.1:696241-696260 None:intergenic 45.0%
ACCATGACTGACCTTGATTC+AGG - chr4.1:694547-694566 None:intergenic 45.0%
ATGAATCAAGGTTGGGACGA+TGG - chr4.1:694198-694217 None:intergenic 45.0%
CGACTTCGATCTTAACCCTT+GGG - chr4.1:693259-693278 None:intergenic 45.0%
CTGGACCTATCAAGAGAAAC+AGG - chr4.1:693499-693518 None:intergenic 45.0%
CTGTAATCGTCGTCGATGTA+AGG - chr4.1:693233-693252 None:intergenic 45.0%
CTGTGAAACATTCCTATCCG+AGG - chr4.1:694786-694805 None:intergenic 45.0%
CTTGAAGCTCCACCTTCTTA+CGG + chr4.1:693167-693186 MS.gene93785:CDS 45.0%
GACCTATCAAGAGAAACAGG+AGG - chr4.1:693496-693515 None:intergenic 45.0%
GCATCTCTTCTTCAACGAGA+CGG - chr4.1:693283-693302 None:intergenic 45.0%
GGCTATGCCTAATGATGCAA+AGG - chr4.1:695867-695886 None:intergenic 45.0%
GGTATTCCTGGCATTTCTCT+AGG + chr4.1:695776-695795 MS.gene93785:intron 45.0%
GTATGCAATATCCTGCTGGT+TGG + chr4.1:694080-694099 MS.gene93785:intron 45.0%
TACTTTGAGCATGACAGCCT+TGG + chr4.1:694052-694071 MS.gene93785:intron 45.0%
TCCTGAATCAAGGTCAGTCA+TGG + chr4.1:694543-694562 MS.gene93785:intron 45.0%
TCGACTTCGATCTTAACCCT+TGG - chr4.1:693260-693279 None:intergenic 45.0%
TGAGAAGAGAAGCAGCAGAA+AGG + chr4.1:696319-696338 MS.gene93785:CDS 45.0%
TGTTGTATGCAACTGCTGGT+TGG + chr4.1:694841-694860 MS.gene93785:intron 45.0%
TTATCATTCCACCAACCAGC+AGG - chr4.1:694094-694113 None:intergenic 45.0%
! AATTGGCTTAAGCCTCGGAT+AGG + chr4.1:694771-694790 MS.gene93785:intron 45.0%
! ATCGGTGCCTCTAGTAAACT+TGG - chr4.1:695027-695046 None:intergenic 45.0%
! TCGGTGCCTCTAGTAAACTT+GGG - chr4.1:695026-695045 None:intergenic 45.0%
! TTTTCTCTCTTTCCAGAGGC+TGG + chr4.1:696408-696427 MS.gene93785:intron 45.0%
!! TGTGCTGTTTCCATGCATCA+GGG - chr4.1:693756-693775 None:intergenic 45.0%
!! TTGGTTTGGTTGCTTGAGTG+AGG + chr4.1:695568-695587 MS.gene93785:intron 45.0%
!! TTGTGCTGTTTCCATGCATC+AGG - chr4.1:693757-693776 None:intergenic 45.0%
!!! GAGGAAATCAGCGGGTTTTT+TGG - chr4.1:693315-693334 None:intergenic 45.0%
AAGCTCCACCTTCTTACGGT+AGG + chr4.1:693171-693190 MS.gene93785:CDS 50.0%
AATCAAGGTTGGGACGATGG+AGG - chr4.1:694195-694214 None:intergenic 50.0%
ACCAGTGCCTCTAGTGAACT+TGG - chr4.1:694247-694266 None:intergenic 50.0%
ATGAGCGTGTAAGAGCTGGT+AGG + chr4.1:693470-693489 MS.gene93785:CDS 50.0%
CAAAACAAAACCCTAGCGGG+CGG - chr4.1:693406-693425 None:intergenic 50.0%
CAGTGCCTCTAGTGAACTTG+GGG - chr4.1:694245-694264 None:intergenic 50.0%
CCAGTGCCTCTAGTGAACTT+GGG - chr4.1:694246-694265 None:intergenic 50.0%
CGGTGCCTCTAGTAAACTTG+GGG - chr4.1:695025-695044 None:intergenic 50.0%
CTAGACCTACCGTAAGAAGG+TGG - chr4.1:693179-693198 None:intergenic 50.0%
GAGTATGAGCGTGTAAGAGC+TGG + chr4.1:693466-693485 MS.gene93785:CDS 50.0%
GGAAGAAGCAGCAGCAGTAA+CGG + chr4.1:693131-693150 MS.gene93785:CDS 50.0%
GGCGACTCCTTTGCATCATT+AGG + chr4.1:695857-695876 MS.gene93785:intron 50.0%
GTAAATGGCAGCCGTAAGAC+CGG - chr4.1:694341-694360 None:intergenic 50.0%
GTACTTGAGCTTGACAGCCT+TGG + chr4.1:694814-694833 MS.gene93785:intron 50.0%
TAACAGTGACAAGGGTGGAC+AGG - chr4.1:696129-696148 None:intergenic 50.0%
TGCCTCTAGTAAACTTGGGG+AGG - chr4.1:695022-695041 None:intergenic 50.0%
TGGCTAGACCTACCGTAAGA+AGG - chr4.1:693182-693201 None:intergenic 50.0%
TTGTCCCCTAGAGAAATGCC+AGG - chr4.1:695785-695804 None:intergenic 50.0%
! GGCCTCCTGTTTCTCTTGAT+AGG + chr4.1:693491-693510 MS.gene93785:CDS 50.0%
! TGCAATATCCTGCTGGTTGG+TGG + chr4.1:694083-694102 MS.gene93785:intron 50.0%
! TGCATCGATGATTTCGGCGT+CGG - chr4.1:693207-693226 None:intergenic 50.0%
! TGTATGCAACTGCTGGTTGG+TGG + chr4.1:694844-694863 MS.gene93785:intron 50.0%
AACTCTTTCTCCGCCCGCTA+GGG + chr4.1:693393-693412 MS.gene93785:intron 55.0%
ACGACGATTACAGCGACCCA+AGG + chr4.1:693240-693259 MS.gene93785:CDS 55.0%
CGACGATTACAGCGACCCAA+GGG + chr4.1:693241-693260 MS.gene93785:CDS 55.0%
GGCCTCCCCAAGTTTACTAG+AGG + chr4.1:695017-695036 MS.gene93785:intron 55.0%
TAACTCTTTCTCCGCCCGCT+AGG + chr4.1:693392-693411 MS.gene93785:intron 55.0%
TCTTTCCAGAGGCTGGCACT+TGG + chr4.1:696415-696434 MS.gene93785:intron 55.0%
TGCCTCTAGTGAACTTGGGG+AGG - chr4.1:694242-694261 None:intergenic 55.0%
! CCCAAGTTCACTAGAGGCAC+TGG + chr4.1:694243-694262 MS.gene93785:intron 55.0%
! CGATGATTTCGGCGTCGGAA+TGG - chr4.1:693202-693221 None:intergenic 55.0%
AGCATCCAAGTGCCAGCCTC+TGG - chr4.1:696423-696442 None:intergenic 60.0%
GGCCTCCCCAAGTTCACTAG+AGG + chr4.1:694237-694256 MS.gene93785:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 693119 696481 693119 ID=MS.gene93785
chr4.1 mRNA 693119 696481 693119 ID=MS.gene93785.t1;Parent=MS.gene93785
chr4.1 exon 693119 693370 693119 ID=MS.gene93785.t1.exon1;Parent=MS.gene93785.t1
chr4.1 CDS 693119 693370 693119 ID=cds.MS.gene93785.t1;Parent=MS.gene93785.t1
chr4.1 exon 693445 693620 693445 ID=MS.gene93785.t1.exon2;Parent=MS.gene93785.t1
chr4.1 CDS 693445 693620 693445 ID=cds.MS.gene93785.t1;Parent=MS.gene93785.t1
chr4.1 exon 693720 693803 693720 ID=MS.gene93785.t1.exon3;Parent=MS.gene93785.t1
chr4.1 CDS 693720 693803 693720 ID=cds.MS.gene93785.t1;Parent=MS.gene93785.t1
chr4.1 exon 696042 696111 696042 ID=MS.gene93785.t1.exon4;Parent=MS.gene93785.t1
chr4.1 CDS 696042 696111 696042 ID=cds.MS.gene93785.t1;Parent=MS.gene93785.t1
chr4.1 exon 696207 696340 696207 ID=MS.gene93785.t1.exon5;Parent=MS.gene93785.t1
chr4.1 CDS 696207 696340 696207 ID=cds.MS.gene93785.t1;Parent=MS.gene93785.t1
chr4.1 exon 696424 696481 696424 ID=MS.gene93785.t1.exon6;Parent=MS.gene93785.t1
chr4.1 CDS 696424 696481 696424 ID=cds.MS.gene93785.t1;Parent=MS.gene93785.t1
Gene Sequence

>MS.gene93785

ATGATGGGAGAAGGAAGAAGCAGCAGCAGTAACGGTGATGTTCTGATGCTTGAAGCTCCACCTTCTTACGGTAGGTCTAGCCATTCCGACGCCGAAATCATCGATGCATTGCCTTACATCGACGACGATTACAGCGACCCAAGGGTTAAGATCGAAGTCGACCGTCTCGTTGAAGAAGAGATGCGTCGCAGCTCCAAAAAACCCGCTGATTTCCTCAAAGATTTTCCACCTCTTCCCTCTTCCAATTTTCAGGTAATAATTAACCACACTAACTAACTCTTTCTCCGCCCGCTAGGGTTTTGTTTTGATTTACGATTCTGCTTCAGGATTATCCAATGATTGGTAGAGAGTATGAGCGTGTAAGAGCTGGTAGGCCTCCTGTTTCTCTTGATAGGTCCAGATATGAATTAGAACCACCACCTGTTAATAAATGGAACGATGAAACTGCTTGGAAACTTGCTATTCAACGAGCTCAACGACTTCTTCAATATCAAACCATCAGGTCTTCACTTTAATTTTTTTTTTTTTTTTAATAAATGTTTTAAGATTCATATTATCATCTACTTGTTTGTTTAACCAACTAGTTTAAGTTAATATACAGAATGGAAAATTTGGAGTTGTTGGTTAAATATGGCCCTGATGCATGGAAACAGCACAATCAACGATTGGAAGTATACTTGTCAAGGTTCGTTTGTTCATCCAATTATACTGTGGCTCATATTTAGAGTAAACTCTTGAATTCCACATTTGTTGCTGTGTGGATTCAGTATGTTTTGTTCACTATTTGCCTGTACTTGTGCAAATCAAATTTTGCTTATTTCATATATTAGCACATGACATGAACATTCTGACATACCTCAGATTAATATACCTCTTGTTGAATTGGTTCTTGAGAGTGATTCTGCAGATAAGAATGTTTCATAGTAAATATGGTACTTTGAGCATGACAGCCTTGGTTGTTGTATGCAATATCCTGCTGGTTGGTGGAATGATAATGTTCTATTTTAATTAATTTTTTTGACTTTAAGACCTTATTAGCTGGAAAAGAAGAAATCTCTTAAGGAAATCATTAACCTCCATCGTCCCAACCTTGATTCATTAAATATGTTCTTGACTCTGGCCTCCCCAAGTTCACTAGAGGCACTGGTCCATATTTTTCATTTTCATTTTCAAACAAAATACAAAGTCTGTTCCTTTAACTGGCAATGATGATAAAAGGCCGGTCTTACGGCTGCCATTTACTTATTGGTAGTAGATTGATTACTTTCATGTTTAGGGTTTTCTGAGCCAGTTTTATTCACCTTTTTCTTTTAAGTTTTTAATTTTCCCTTTTCAAAACAAGGTTGAAGTTCTACATCTTTGTCTTCAACCATGGGTCATTCTATTTTTTTATGAATAAAGTCAATGTTTTCCTTCTATTTTTCTCCTGAATCAAGGTCAGTCATGGTTGAAATTCTTCATATTTTTCTCGAAGAAATCTAGTGACAAAAATGAGATCAGTGCTCTGCGGTAAATTATTTTGTACCTGTGCATCATTTGCTTACTTATACGTTAGCTAAAGAAAATTCTGACATACCTGCATTTGTTCTGTATGACTGAATTGCACATAGAATACTTTGTTCCTTTCTGTTTTAAATTTCTTCCCAAAAAGCAATTGGCTTAAGCCTCGGATAGGAATGTTTCACAGCAAATATGGTACTTGAGCTTGACAGCCTTGGTTGTTGTTGTATGCAACTGCTGGTTGGTGGAATGATAATGTTCTATTTTTAATTCTTGTTTTTTTTTTGGATTTTAAGACCTTATTAGCTACGGAAGAAGAAATCTCTTGAGGAAATCATTGATTTCTACATTCTTCTTAATCGTTTCAACCTTGATTCATTAAATGTGTTCTTGACTCTGGCCTCCCCAAGTTTACTAGAGGCACCGATCCATATTTTTCAGTTTCCTTTTCAAACAAAACACAAAGTATGTTCCTTTAACTGGCAATGATGATAAAAGGCCGGTCTTACGGCTGCCATTTACTTCTATGCTAGTAGATTGATTACTTTCATGTTTAGGGTTTTCTGAGCCAGTTTTATTCACCTTTTTCTTCTAAGTTTTTAATTTTTCCTTTTTTCAAAACAAGGTTGAATTTTCTACTTCTATGTCTTCTATCATGGCTCATTCTATTTTTTAATGAATAAGGTCAATGATTTCCTTCTATTTTTCTCCTGAATCCAAAGGTCAATCATGGTTGAAATTCTTCATATTTTTCTCTTGGAGAAATCTAGTGACAAAAATGAGATCAGTTCACTGTGGTGAATTATTTTGTTGTTGTATGCAATATCCTGCGGATATGAATGTTTCACATAAATATGGTAGTTGAGGTTGACAGCCTTTGATGTGCTCGAGACATATTCCCTTAAGTTCCTAATTAACAAGTATTCAAACCTGACTTTATTCTTCCATATTGGTTTGGTTGCTTGAGTGAGGCTTACTTGAGTGTCTTCTAAAGAAACTATTAGCAGATTCTTTAAGTCTCTTGCTTGGAGCATTTCTTAGTTCAGTGCTTCACAATACTCGTTACCTTGTACATAAAATGCACGCAACATATTATTAATTCCTTAACACATGAGTTTCACCCAATAGGAGAGTGAGTTTTAGAGTGTTAGAAAGAGGGTATTCCTGGCATTTCTCTAGGGGACAAACATCTTTATCCCTAATTGCAGTCTAAGCAACTCCTTTTTCTTCACATAAAAGGCGACTCCTTTGCATCATTAGGCATAGCCCAATGCTCTCAAAATTACTAGTCTTAAGGACTGTATTTCATAGCATTATATCCTTTTTCTTTCATCGATTTTAATTTTGAGGAACTATTGTTCTGTTGTCACTTACATATCTCTTTTCGTATAGCATTGGTTACAGTTGTGTTATCTATTTTCAGAATGCAGAAATTAGCTCAGGAACAAAATGATAAGATTGAAAAAGTTAACCGTGAAAGGAAATATCATCAGGTGAATATGACTTTCCTGTCCACCCTTGTCACTGTTATTTTTAAAATTCTTGTTAAGTTTACTTAAAGTCACATTTTTTTAATAACTATTGTCAGCAAAACACCGCATATGAGCTTAATGCTTTGTCCATGCAATGGAATGAGCTCTGTCAGAAAAATATAGACATCAAAGTTGTGTGTGCTTCAGTTGAAACTCACTTAAATGAACTGAGAAGAGAAGCAGCAGAAAGGTAACTCAGCCTTGTAAATTTTGTTTTTCTTGGCTGATTTCATCTAGTTTTGGTGTGTAATTAGTTTTTTTCTCTCTTTCCAGAGGCTGGCACTTGGATGCTATCACTGAAAATGGCCAACTGGCAAATTCGGAATCATGA
Protein sequence

>MS.gene93785.t1

MMGEGRSSSSNGDVLMLEAPPSYGRSSHSDAEIIDALPYIDDDYSDPRVKIEVDRLVEEEMRRSSKKPADFLKDFPPLPSSNFQDYPMIGREYERVRAGRPPVSLDRSRYELEPPPVNKWNDETAWKLAIQRAQRLLQYQTIRMENLELLVKYGPDAWKQHNQRLEVYLSRMQKLAQEQNDKIEKVNRERKYHQQNTAYELNALSMQWNELCQKNIDIKVVCASVETHLNELRREAAERGWHLDAITENGQLANSES