AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050374

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050374.t1 MTR_7g115550 95.310 725 28 1 4 728 8 726 0.0 1338
MS.gene050374.t1 MTR_7g115550 95.330 728 28 1 4 731 8 729 0.0 1337
MS.gene050374.t1 MTR_5g010470 88.033 727 36 4 4 730 8 683 0.0 1191
MS.gene050374.t1 MTR_7g061330 36.364 110 64 2 4 112 62 166 8.03e-13 72.0
MS.gene050374.t1 MTR_7g461410 36.364 110 64 2 4 112 62 166 8.03e-13 72.0
MS.gene050374.t1 MTR_7g061330 36.364 110 64 2 4 112 26 130 9.77e-13 71.6
MS.gene050374.t1 MTR_3g110028 38.462 78 48 0 4 81 86 163 2.06e-11 68.2
MS.gene050374.t1 MTR_6g027340 40.789 76 45 0 6 81 19 94 2.55e-11 63.9
MS.gene050374.t1 MTR_8g468380 32.353 102 64 1 3 104 22 118 3.74e-11 66.2
MS.gene050374.t1 MTR_6g027360 40.741 81 48 0 1 81 76 156 3.87e-11 65.5
MS.gene050374.t1 MTR_7g451170 38.750 80 49 0 4 83 192 271 5.08e-11 65.9
MS.gene050374.t1 MTR_1g026870 32.727 110 68 2 4 112 34 138 9.21e-11 65.9
MS.gene050374.t1 MTR_5g038910 32.456 114 68 2 3 116 26 130 9.85e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050374.t1 AT1G09770 76.066 727 163 4 4 727 8 726 0.0 1069
MS.gene050374.t1 AT3G18100 35.294 102 59 2 4 104 547 642 1.79e-13 74.7
MS.gene050374.t1 AT3G18100 34.653 101 61 2 6 106 498 593 2.88e-11 67.4
MS.gene050374.t1 AT3G18100 35.294 102 59 2 4 104 499 594 3.17e-13 73.9
MS.gene050374.t1 AT3G18100 34.653 101 61 2 6 106 450 545 5.75e-11 66.6
MS.gene050374.t1 AT3G18100 35.294 102 59 2 4 104 386 481 4.03e-13 73.6
MS.gene050374.t1 AT3G18100 34.653 101 61 2 6 106 337 432 7.78e-11 65.9
MS.gene050374.t1 AT5G02320 34.862 109 67 1 4 112 76 180 4.46e-13 72.8
MS.gene050374.t1 AT5G02320 34.862 109 67 1 4 112 76 180 4.46e-13 72.8
MS.gene050374.t1 AT5G02320 34.862 109 67 1 4 112 16 120 5.11e-13 72.4
MS.gene050374.t1 AT3G18100 35.294 102 59 2 4 104 334 429 5.20e-13 72.8
MS.gene050374.t1 AT5G11510 35.455 110 65 2 4 112 30 134 4.43e-12 70.5
MS.gene050374.t1 AT5G11510 35.455 110 65 2 4 112 30 134 4.43e-12 70.5
MS.gene050374.t1 AT5G11510 35.455 110 65 2 4 112 30 134 4.43e-12 70.5
MS.gene050374.t1 AT5G11510 35.455 110 65 2 4 112 30 134 9.57e-12 68.9
MS.gene050374.t1 AT5G11510 35.455 110 65 2 4 112 30 134 9.81e-12 68.9
MS.gene050374.t1 AT4G18770 38.889 90 50 1 4 93 218 302 1.43e-11 67.8
MS.gene050374.t1 AT3G09370 34.545 110 66 2 4 112 84 188 1.78e-11 67.8
MS.gene050374.t1 AT3G09370 34.545 110 66 2 4 112 79 183 1.83e-11 67.8
MS.gene050374.t1 AT4G33450 38.462 78 48 0 4 81 20 97 2.15e-11 65.5
MS.gene050374.t1 AT3G09370 34.545 110 66 2 4 112 92 196 2.63e-11 67.4
MS.gene050374.t1 AT5G17800 40.000 80 48 0 2 81 92 171 9.00e-11 64.3
MS.gene050374.t1 AT5G40360 37.179 78 49 0 4 81 159 236 9.20e-11 64.7

Find 213 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTCTTTCTAACTGTTTC+TGG 0.008572 5.2:+3628537 None:intergenic
TTCTGGGTCATTCAGCTTCT+TGG 0.153986 5.2:+3628554 None:intergenic
TGCCGGCGCCATACCACTTC+TGG 0.177175 5.2:+3628122 None:intergenic
ATTTCATAACAGCAGCTTTC+AGG 0.225007 5.2:+3629360 None:intergenic
CAGCATCTCTGGAGCTTATT+AGG 0.228626 5.2:-3627753 MS.gene050374:CDS
GGAGTAAGGCCTGCACTTCC+AGG 0.240853 5.2:+3628144 None:intergenic
TTGGGTGTCTGTGGATAATT+AGG 0.241982 5.2:+3628384 None:intergenic
CATGATGGAAGCTGAAAATT+TGG 0.248037 5.2:-3628301 MS.gene050374:CDS
GTTCTCTGAGGAGTTCTTAA+AGG 0.283026 5.2:+3628348 None:intergenic
AAAGCTGCTGTTATGAAATA+TGG 0.287568 5.2:-3629356 MS.gene050374:CDS
GAATTGGTTGCATCACTATC+TGG 0.302904 5.2:+3627923 None:intergenic
AAAGGAGCTTCTAGCCTTAC+TGG 0.309592 5.2:-3627650 MS.gene050374:CDS
GGTCGCGCAGGCTTCGATTC+AGG 0.310060 5.2:+3629002 None:intergenic
AGTCCACTCAGTCTTCTTAA+TGG 0.314486 5.2:+3629244 None:intergenic
CCCTGTCATAGAAGATTATC+AGG 0.315605 5.2:-3627539 MS.gene050374:CDS
CCATTGCCTCACGAAGATAC+TGG 0.317289 5.2:+3626820 None:intergenic
TGGGGTCACTCCTAAGAAAA+AGG 0.319124 5.2:-3628208 MS.gene050374:CDS
TCTTCAGCATCTTCTTGAAT+TGG 0.331097 5.2:+3627907 None:intergenic
GTTTGAGAAGAGGCCTCCTC+TGG 0.334561 5.2:-3628751 MS.gene050374:CDS
ATCTCTTTCTAACTGTTTCT+GGG 0.335857 5.2:+3628538 None:intergenic
ATCTCGTCGGCTTGCTCAAT+AGG 0.342238 5.2:+3627679 None:intergenic
TCAAGTTCTTCTATAGTAGT+AGG 0.344179 5.2:+3628678 None:intergenic
CTCCGAAGGGAACTCCTCTC+AGG 0.358383 5.2:-3628074 MS.gene050374:CDS
TAGGCCTCCTCCAGCATCTC+TGG 0.367513 5.2:-3627764 MS.gene050374:CDS
GCTTGAGGAGATAGCAAATA+AGG 0.373696 5.2:-3627605 MS.gene050374:CDS
CCAAAATGAGCATAGGTGTT+TGG 0.385146 5.2:-3629403 None:intergenic
TCTCGTCGGCTTGCTCAATA+GGG 0.387795 5.2:+3627680 None:intergenic
AGGCCCATTTGCTGCTCGTT+TGG 0.388358 5.2:+3627567 None:intergenic
TCAGATTTCCGATCAAGAAT+TGG 0.389053 5.2:-3628493 MS.gene050374:CDS
GCAGGCTTCGATTCAGGATT+CGG 0.393602 5.2:+3629008 None:intergenic
CCTTGACGCCGCTTGTGTTA+AGG 0.393639 5.2:-3629087 MS.gene050374:CDS
AGGCTGCTGGGATTGATATA+AGG 0.409355 5.2:-3628821 MS.gene050374:CDS
TCTGCAACCCCTGGAAGTGC+AGG 0.411721 5.2:-3628153 MS.gene050374:CDS
AGAGATGCTGGAGGAGGCCT+AGG 0.415805 5.2:+3627766 None:intergenic
TATGGTCTATCAAGTGTTGC+TGG 0.416170 5.2:-3626711 MS.gene050374:CDS
GCCGGCGCCATACCACTTCT+GGG 0.416494 5.2:+3628123 None:intergenic
GGATCCAAACGAGCAGCAAA+TGG 0.417377 5.2:-3627571 MS.gene050374:CDS
AAAGGAGTCATGGCTTGGTT+GGG 0.420072 5.2:+3628366 None:intergenic
GCTACATATCAATGAAGATA+TGG 0.420115 5.2:-3628046 MS.gene050374:CDS
ACCCACAATGGTAGCACTAA+TGG 0.421586 5.2:+3629148 None:intergenic
GAGGGAGTCGGCAGTGGGAT+TGG 0.422499 5.2:+3628174 None:intergenic
TAAGGCAGCGGAGGAGGAAA+AGG 0.424744 5.2:-3628803 MS.gene050374:CDS
TTGGATGAAATTGCCAAGTT+GGG 0.429250 5.2:-3628474 MS.gene050374:CDS
AGCTTTCAGGATTTCATCCT+CGG 0.436489 5.2:+3629373 None:intergenic
CAGTATCTTCGTGAGGCAAT+GGG 0.437430 5.2:-3626819 MS.gene050374:CDS
GTTGCAGAGGGAGTCGGCAG+TGG 0.438871 5.2:+3628168 None:intergenic
ATTGGATGAAATTGCCAAGT+TGG 0.444138 5.2:-3628475 MS.gene050374:CDS
AAGAACTTGAGGGGAAAAGA+AGG 0.444403 5.2:-3628665 MS.gene050374:CDS
GCAAATAAGGAGAGAGAGAA+AGG 0.446065 5.2:-3627592 MS.gene050374:CDS
ACTAATGGTTCTCCAACTAA+TGG 0.446782 5.2:+3629163 None:intergenic
CTAATAAGCTCCAGAGATGC+TGG 0.447893 5.2:+3627754 None:intergenic
CCATACCACTTCTGGGAGTA+AGG 0.448683 5.2:+3628130 None:intergenic
ATCCTCTTCAATCTTCTCCT+CGG 0.449369 5.2:+3627882 None:intergenic
AAGAGAGAATTGAAGGCTGC+TGG 0.451049 5.2:-3628834 MS.gene050374:CDS
TCCTGATAATCTTCTATGAC+AGG 0.451422 5.2:+3627538 None:intergenic
TTGATAGACCATATGTGTTA+CGG 0.451766 5.2:+3626721 None:intergenic
CCGACTCCCTCTGCAACCCC+TGG 0.454253 5.2:-3628162 MS.gene050374:CDS
TCATACTCATCCATATCAAC+AGG 0.454291 5.2:+3628975 None:intergenic
TGATAGACCATATGTGTTAC+GGG 0.454903 5.2:+3626722 None:intergenic
TCCCCTGGACGCAATTTCCT+AGG 0.458724 5.2:+3629038 None:intergenic
TTTGATACCCGTAACACATA+TGG 0.465342 5.2:-3626729 MS.gene050374:CDS
CAGAAATTCCGTTTGAGAAG+AGG 0.469889 5.2:-3628761 MS.gene050374:CDS
TGCAGAGAGGCAAGATGCCC+CGG 0.470266 5.2:-3628592 MS.gene050374:CDS
TAAAGGAGTCATGGCTTGGT+TGG 0.470630 5.2:+3628365 None:intergenic
GAGTCTCAGACACCACTGTT+AGG 0.471291 5.2:-3628267 MS.gene050374:CDS
CTGAAAATTTGGCCAGGCTG+AGG 0.471566 5.2:-3628290 MS.gene050374:CDS
TTCTTAAAGGAGTCATGGCT+TGG 0.472192 5.2:+3628361 None:intergenic
TGGCATGCAGAATAGCAGCC+GGG 0.473211 5.2:+3628574 None:intergenic
TTGATATAAGGCAGCGGAGG+AGG 0.474103 5.2:-3628809 MS.gene050374:CDS
AGCCAACCGAGCACATGCTT+CGG 0.476372 5.2:+3628938 None:intergenic
TGATGTTGCTGATGAGGATA+GGG 0.479449 5.2:-3628721 MS.gene050374:CDS
AATTTCATCCAATTCTTGAT+CGG 0.479560 5.2:+3628485 None:intergenic
GCGACCCGATCCTGTTGATA+TGG 0.481942 5.2:-3628985 MS.gene050374:CDS
AATCTGAAGGATGCAACTCC+GGG 0.484835 5.2:+3628235 None:intergenic
CCAGTATCTTCGTGAGGCAA+TGG 0.484875 5.2:-3626820 MS.gene050374:CDS
TGTCTGAGACTCCCTCAGCC+TGG 0.484905 5.2:+3628278 None:intergenic
AACGATCAAAAGTCATTCAA+AGG 0.485643 5.2:-3627795 MS.gene050374:CDS
TCCTCTTCAATCTTCTCCTC+GGG 0.488427 5.2:+3627883 None:intergenic
ACCATTAGTGCTACCATTGT+GGG 0.490042 5.2:-3629149 MS.gene050374:CDS
GAGTAAGGCCTGCACTTCCA+GGG 0.490905 5.2:+3628145 None:intergenic
AGAAGAAGAGGCACGGCAGA+AGG 0.493242 5.2:-3627830 MS.gene050374:CDS
CTCCTTTCTTATCATCTCGT+CGG 0.493278 5.2:+3627666 None:intergenic
TCATTCAAAGGGAACTTCCT+AGG 0.493709 5.2:-3627783 MS.gene050374:CDS
TGCACTTCCAGGGGTTGCAG+AGG 0.493979 5.2:+3628155 None:intergenic
AAATCTGAAGGATGCAACTC+CGG 0.494050 5.2:+3628234 None:intergenic
ACGAGTAGCACTGCTGCCTT+CGG 0.495690 5.2:+3628416 None:intergenic
TTCCGAAGCATGTGCTCGGT+TGG 0.496711 5.2:-3628940 MS.gene050374:CDS
TCTTCTATGACAGGGATAGC+AGG 0.496725 5.2:+3627547 None:intergenic
TTGCAGAGGGAGTCGGCAGT+GGG 0.497407 5.2:+3628169 None:intergenic
CTCAGTGGAGAACCATTAGT+TGG 0.497414 5.2:-3629175 MS.gene050374:CDS
GTCTCCGTCAGCTCTTATCA+AGG 0.499862 5.2:+3627726 None:intergenic
GAAGTGGTATGGCGCCGGCA+AGG 0.499877 5.2:-3628119 MS.gene050374:CDS
TGCTTTCCGAAGCATGTGCT+CGG 0.502006 5.2:-3628944 MS.gene050374:CDS
GTGAGAAGTAAGCTGGATGA+TGG 0.502979 5.2:-3626645 MS.gene050374:CDS
CTCATCCATATCAACAGGAT+CGG 0.503902 5.2:+3628980 None:intergenic
AAGTAAGCTGGATGATGGGA+AGG 0.506469 5.2:-3626640 MS.gene050374:CDS
ATAGCATCTCCTTTACTAGC+TGG 0.506651 5.2:+3628324 None:intergenic
AGAGGATATGTCTGATAGAA+TGG 0.507303 5.2:-3627866 MS.gene050374:CDS
AGCTCCAGAGATGCTGGAGG+AGG 0.508047 5.2:+3627760 None:intergenic
TGCAGATTTGCAAACGAGTA+GGG 0.508803 5.2:+3629307 None:intergenic
TCCCTGAGAGGAGTTCCCTT+CGG 0.510495 5.2:+3628072 None:intergenic
GATGATTCGACTTGAGAAGA+AGG 0.511403 5.2:-3626613 MS.gene050374:CDS
TGATAGAATGGCTAGAGAAA+AGG 0.511992 5.2:-3627854 MS.gene050374:CDS
TTGGCATGCAGAATAGCAGC+CGG 0.514383 5.2:+3628573 None:intergenic
CAGAGAACACCAGCTAGTAA+AGG 0.515217 5.2:-3628333 MS.gene050374:CDS
AAGTGGTATGGCGCCGGCAA+GGG 0.516862 5.2:-3628118 MS.gene050374:CDS
CATTGCCTCACGAAGATACT+GGG 0.519621 5.2:+3626821 None:intergenic
TGGGTGTCTGTGGATAATTA+GGG 0.519886 5.2:+3628385 None:intergenic
TTGATGTTGCTGATGAGGAT+AGG 0.524642 5.2:-3628722 MS.gene050374:CDS
TCGGCTTGCTCAATAGGGGT+TGG 0.524733 5.2:+3627685 None:intergenic
TGAAAATTTGGCCAGGCTGA+GGG 0.525084 5.2:-3628289 MS.gene050374:CDS
GACCCTAGGAAATTGCGTCC+AGG 0.525314 5.2:-3629041 MS.gene050374:CDS
GGTTATGCCATGGACCTTGT+GGG 0.528280 5.2:-3628453 MS.gene050374:CDS
CAAGTTCTTCTATAGTAGTA+GGG 0.528361 5.2:+3628679 None:intergenic
AGAGGAGGCCTCTTCTCAAA+CGG 0.529206 5.2:+3628753 None:intergenic
TGAGAATGTGAGAAGTAAGC+TGG 0.529343 5.2:-3626652 MS.gene050374:CDS
GCTTGCTCAATAGGGGTTGG+TGG 0.531592 5.2:+3627688 None:intergenic
CACTCAGTCTTCTTAATGGA+AGG 0.532258 5.2:+3629248 None:intergenic
GCACTTCCAGGGGTTGCAGA+GGG 0.532965 5.2:+3628156 None:intergenic
TCATCCATATCAACAGGATC+GGG 0.534614 5.2:+3628981 None:intergenic
AGCATTGTCATGCTCCAGTA+AGG 0.535038 5.2:+3627636 None:intergenic
ACCCTAGGAAATTGCGTCCA+GGG 0.536757 5.2:-3629040 MS.gene050374:CDS
TGCCAAGTTGGGTTATGCCA+TGG 0.539994 5.2:-3628463 MS.gene050374:CDS
GATCCAAACGAGCAGCAAAT+GGG 0.542547 5.2:-3627570 MS.gene050374:CDS
TTGCAGATTTGCAAACGAGT+AGG 0.543492 5.2:+3629306 None:intergenic
AGAGAGAATTGAAGGCTGCT+GGG 0.549517 5.2:-3628833 MS.gene050374:CDS
TGATCGGAAATCTGAGGTGG+TGG 0.551298 5.2:+3628501 None:intergenic
CCTTACTCCCAGAAGTGGTA+TGG 0.551739 5.2:-3628130 MS.gene050374:CDS
AGCCGACGAGATGATAAGAA+AGG 0.552168 5.2:-3627668 MS.gene050374:CDS
GAAAAGAAGGATTGATGTTG+AGG 0.552508 5.2:-3628652 MS.gene050374:CDS
GGTATGGCGCCGGCAAGGGA+TGG 0.553823 5.2:-3628114 MS.gene050374:CDS
AGGAGTTCTTAAAGGAGTCA+TGG 0.554344 5.2:+3628356 None:intergenic
GGGTTATGCCATGGACCTTG+TGG 0.554365 5.2:-3628454 MS.gene050374:CDS
CCAGGGGTTGCAGAGGGAGT+CGG 0.554374 5.2:+3628162 None:intergenic
TCCCAGAAGTGGTATGGCGC+CGG 0.554437 5.2:-3628124 MS.gene050374:CDS
AACCATTAGTGCTACCATTG+TGG 0.555517 5.2:-3629150 MS.gene050374:CDS
ATCGCTGCCCACAAGGTCCA+TGG 0.556575 5.2:+3628446 None:intergenic
TGAGAAAGCAAGACGTTGCA+AGG 0.556741 5.2:-3628623 MS.gene050374:CDS
GGATTCGGATCAATCTCCCC+TGG 0.561665 5.2:+3629023 None:intergenic
CCCCTGGACGCAATTTCCTA+GGG 0.562986 5.2:+3629039 None:intergenic
TCAACAGGATCGGGTCGCGC+AGG 0.566355 5.2:+3628990 None:intergenic
ACGATCAAAAGTCATTCAAA+GGG 0.567785 5.2:-3627794 MS.gene050374:CDS
CAAAACAGTGTAAAGCTCGT+TGG 0.569337 5.2:-3629286 MS.gene050374:CDS
ACTACTATAGAAGAACTTGA+GGG 0.571318 5.2:-3628675 MS.gene050374:CDS
GTCCATGGCATAACCCAACT+TGG 0.572980 5.2:+3628461 None:intergenic
TAAAGGAGATGCTATCATGA+TGG 0.573963 5.2:-3628316 MS.gene050374:CDS
TTACTAGCTGGTGTTCTCTG+AGG 0.578802 5.2:+3628336 None:intergenic
TAGAGAAAAGGCAGAAGAAG+AGG 0.579560 5.2:-3627842 MS.gene050374:CDS
CCTTAACACAAGCGGCGTCA+AGG 0.579579 5.2:+3629087 None:intergenic
ATGAACCTGGTGATGACCCT+AGG 0.580871 5.2:-3629055 MS.gene050374:CDS
TCCGAAGGGAACTCCTCTCA+GGG 0.580985 5.2:-3628073 MS.gene050374:CDS
TTATTTGCTATCTCCTCAAG+CGG 0.583374 5.2:+3627607 None:intergenic
ATGGCTTGGTTGGGTGTCTG+TGG 0.585691 5.2:+3628375 None:intergenic
AAGGTTACATTACTCACGCA+AGG 0.586053 5.2:-3626594 MS.gene050374:CDS
AGTAAGGCCTGCACTTCCAG+GGG 0.586407 5.2:+3628146 None:intergenic
AACTCCTTGATAAGAGCTGA+CGG 0.589304 5.2:-3627730 MS.gene050374:CDS
CCTGATAATCTTCTATGACA+GGG 0.589597 5.2:+3627539 None:intergenic
GTAAAGCTCGTTGGTATGAG+TGG 0.590546 5.2:-3629277 MS.gene050374:CDS
GACTGAGTGGACTCGAGAAG+AGG 0.592125 5.2:-3629234 MS.gene050374:CDS
TGCTTCAACAAAATCCTCGA+GGG 0.592155 5.2:+3626782 None:intergenic
CTCCTTCGCAGTGTCTTGAA+CGG 0.594673 5.2:-3629121 MS.gene050374:CDS
TGCTGATGAGGATAGGGAGG+TGG 0.595277 5.2:-3628715 MS.gene050374:CDS
AGTGTTGCCAAAATGAGCAT+AGG 0.596521 5.2:-3629410 None:intergenic
TGAGAAGTAAGCTGGATGAT+GGG 0.598085 5.2:-3626644 MS.gene050374:CDS
TACTACTATAGAAGAACTTG+AGG 0.598439 5.2:-3628676 MS.gene050374:CDS
AAAAGGCAGAAGAAGAGGCA+CGG 0.599691 5.2:-3627837 MS.gene050374:CDS
TTTGAGAAGAGGCCTCCTCT+GGG 0.600719 5.2:-3628750 MS.gene050374:CDS
CTAATGGTTCTCCACTGAGT+AGG 0.603402 5.2:+3629179 None:intergenic
TGGAAGCTGAAAATTTGGCC+AGG 0.607474 5.2:-3628296 MS.gene050374:CDS
GCTCGGTTGGCTAACACGAA+AGG 0.608258 5.2:-3628927 MS.gene050374:CDS
TCTCAGACACCACTGTTAGG+AGG 0.609284 5.2:-3628264 MS.gene050374:CDS
AGCGGAGGAGGAAAAGGAGA+GGG 0.609580 5.2:-3628797 MS.gene050374:CDS
TGAGTTCATCGCTGCCCACA+AGG 0.613064 5.2:+3628439 None:intergenic
AGAAACAGCTTGAGGAAGCG+AGG 0.613924 5.2:-3628878 MS.gene050374:CDS
CAATGCTAAGTATCCGCTTG+AGG 0.613956 5.2:-3627620 MS.gene050374:CDS
AATTTCCTAGGGTCATCACC+AGG 0.619740 5.2:+3629050 None:intergenic
CAGCGGAGGAGGAAAAGGAG+AGG 0.619802 5.2:-3628798 MS.gene050374:CDS
CTGGGATTGATATAAGGCAG+CGG 0.621106 5.2:-3628815 MS.gene050374:CDS
ACTGTTAGGAGGAGAGAACC+CGG 0.623267 5.2:-3628253 MS.gene050374:CDS
GCAGGCCTTACTCCCAGAAG+TGG 0.624004 5.2:-3628135 MS.gene050374:CDS
CGAAGGAGTACGACCCACAA+TGG 0.624367 5.2:+3629136 None:intergenic
CCCTAGGAAATTGCGTCCAG+GGG 0.631019 5.2:-3629039 MS.gene050374:CDS
CTCGTCGGCTTGCTCAATAG+GGG 0.633774 5.2:+3627681 None:intergenic
AACAGCTTGAGGAAGCGAGG+AGG 0.635219 5.2:-3628875 MS.gene050374:CDS
GGCAGCGATGAACTCACCGA+AGG 0.635534 5.2:-3628432 MS.gene050374:CDS
GGCATGCAGAATAGCAGCCG+GGG 0.639810 5.2:+3628575 None:intergenic
AAGGACGATAACTATGAACC+TGG 0.641325 5.2:-3629068 MS.gene050374:CDS
TGTTGCTGATGAGGATAGGG+AGG 0.645550 5.2:-3628718 MS.gene050374:CDS
AGAAGATGCTGAAGAGCCCG+AGG 0.646686 5.2:-3627899 MS.gene050374:CDS
CTACTATAGAAGAACTTGAG+GGG 0.654103 5.2:-3628674 MS.gene050374:CDS
GCCCGAGGAGAAGATTGAAG+AGG 0.654306 5.2:-3627884 MS.gene050374:CDS
ATAGTGCTAGACTCGAGCTA+CGG 0.656913 5.2:-3628014 MS.gene050374:CDS
GTTATCGTCCTTAACACAAG+CGG 0.659717 5.2:+3629079 None:intergenic
AATTCTTGATCGGAAATCTG+AGG 0.665249 5.2:+3628495 None:intergenic
AGCTAGGGAGAAACAGCTTG+AGG 0.665565 5.2:-3628886 MS.gene050374:CDS
TACCGTTCAAGACACTGCGA+AGG 0.666095 5.2:+3629119 None:intergenic
AGGTTACATTACTCACGCAA+GGG 0.667391 5.2:-3626593 MS.gene050374:CDS
CTAAGCTTACGCCTACTCAG+TGG 0.671805 5.2:-3629190 MS.gene050374:CDS
ACATCAAAATAACCCAGAGG+AGG 0.672004 5.2:+3628738 None:intergenic
TCTTGATCGGAAATCTGAGG+TGG 0.678038 5.2:+3628498 None:intergenic
GGGTTCTCTCCTCCTAACAG+TGG 0.680208 5.2:+3628255 None:intergenic
CTTCCATTAAGAAGACTGAG+TGG 0.680364 5.2:-3629247 MS.gene050374:CDS
TGAAGCCCAGTATCTTCGTG+AGG 0.688109 5.2:-3626826 MS.gene050374:CDS
GTGCTTCAACAAAATCCTCG+AGG 0.688213 5.2:+3626781 None:intergenic
ATATGTAGCTCATCCCTGAG+AGG 0.688581 5.2:+3628060 None:intergenic
AGTATCTTCGTGAGGCAATG+GGG 0.691770 5.2:-3626818 MS.gene050374:CDS
GGATTGATATAAGGCAGCGG+AGG 0.699913 5.2:-3628812 MS.gene050374:CDS
ATAAGCTCCAGAGATGCTGG+AGG 0.701115 5.2:+3627757 None:intergenic
TGATATGGATGAGTATGAGA+AGG 0.714958 5.2:-3628970 MS.gene050374:CDS
TGTCATAGAAGATTATCAGG+AGG 0.727087 5.2:-3627536 MS.gene050374:CDS
GCATGAAAATGATTCCCTCG+AGG 0.731393 5.2:-3626796 MS.gene050374:CDS
CAAGGAATAAAATTGCAGAG+AGG 0.745082 5.2:-3628605 MS.gene050374:CDS
GCAACATCAAAATAACCCAG+AGG 0.761769 5.2:+3628735 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGACAAAAATAAAATTAA+AGG + chr5.2:3628501-3628520 None:intergenic 10.0%
!! TTATAATCATTTGATTAATT+AGG - chr5.2:3628845-3628864 MS.gene050374:CDS 10.0%
!! ATTATAACTGAAAAAAAACA+AGG + chr5.2:3628832-3628851 None:intergenic 15.0%
!! AAGTTTGTATTACTATCATT+CGG + chr5.2:3628983-3629002 None:intergenic 20.0%
!! ATAATAATTAACAAGCAGTT+TGG + chr5.2:3629006-3629025 None:intergenic 20.0%
!! TTGTCTGATTATTAATTACT+TGG - chr5.2:3628653-3628672 MS.gene050374:CDS 20.0%
!!! ACAAAGTACTAATAATTTCA+AGG + chr5.2:3628637-3628656 None:intergenic 20.0%
! AATTTCATCCAATTCTTGAT+CGG + chr5.2:3627488-3627507 None:intergenic 25.0%
! GAAATATGGTAAAAATCAGT+GGG - chr5.2:3626628-3626647 MS.gene050374:CDS 25.0%
! TAGGGAATAAATGTTGTAAA+AGG + chr5.2:3628946-3628965 None:intergenic 25.0%
! TGAAATATGGTAAAAATCAG+TGG - chr5.2:3626627-3626646 MS.gene050374:CDS 25.0%
!! ATGTGAATTTCCTTTTTCTT+AGG + chr5.2:3627775-3627794 None:intergenic 25.0%
!! ATTTTTACATAAGATTCCAG+TGG + chr5.2:3628722-3628741 None:intergenic 25.0%
!! TTTTATCTGTTGTTCTCAAT+AGG - chr5.2:3629102-3629121 MS.gene050374:CDS 25.0%
AAAAAGCAAAAAGGAAAGCT+AGG - chr5.2:3627068-3627087 MS.gene050374:intron 30.0%
AAAAGCAAAAAGGAAAGCTA+GGG - chr5.2:3627069-3627088 MS.gene050374:intron 30.0%
AAAGCTGCTGTTATGAAATA+TGG - chr5.2:3626614-3626633 MS.gene050374:CDS 30.0%
AACGATCAAAAGTCATTCAA+AGG - chr5.2:3628175-3628194 MS.gene050374:CDS 30.0%
AATATGCAACTAGCTCATAA+AGG + chr5.2:3628789-3628808 None:intergenic 30.0%
ACAGAAAAAGAGAGAATTGA+AGG - chr5.2:3627129-3627148 MS.gene050374:intron 30.0%
ACGATCAAAAGTCATTCAAA+GGG - chr5.2:3628176-3628195 MS.gene050374:CDS 30.0%
ACTACTATAGAAGAACTTGA+GGG - chr5.2:3627295-3627314 MS.gene050374:intron 30.0%
CAAGTTCTTCTATAGTAGTA+GGG + chr5.2:3627294-3627313 None:intergenic 30.0%
CATCTCCATAGTCAAATTTA+AGG + chr5.2:3629035-3629054 None:intergenic 30.0%
CGAAAGGTAAAAAAGCAAAA+AGG - chr5.2:3627059-3627078 MS.gene050374:intron 30.0%
GCTACATATCAATGAAGATA+TGG - chr5.2:3627924-3627943 MS.gene050374:CDS 30.0%
TACTACTATAGAAGAACTTG+AGG - chr5.2:3627294-3627313 MS.gene050374:intron 30.0%
TATGAGCTAGTTGCATATTA+TGG - chr5.2:3628790-3628809 MS.gene050374:CDS 30.0%
TCAAGTTCTTCTATAGTAGT+AGG + chr5.2:3627295-3627314 None:intergenic 30.0%
TGACTCCTTAAATTTGACTA+TGG - chr5.2:3629027-3629046 MS.gene050374:CDS 30.0%
TTGATAGACCATATGTGTTA+CGG + chr5.2:3629252-3629271 None:intergenic 30.0%
! ATCTCTTTCTAACTGTTTCT+GGG + chr5.2:3627435-3627454 None:intergenic 30.0%
!! ACTATCTGGTACTCATTTTT+AGG + chr5.2:3628036-3628055 None:intergenic 30.0%
!!! TTATTTTGATGTTGCTGATG+AGG - chr5.2:3627243-3627262 MS.gene050374:intron 30.0%
AAGATTCCAGTGGTTTCAAA+GGG + chr5.2:3628712-3628731 None:intergenic 35.0%
AATTCTTGATCGGAAATCTG+AGG + chr5.2:3627478-3627497 None:intergenic 35.0%
ACTAATGGTTCTCCAACTAA+TGG + chr5.2:3626810-3626829 None:intergenic 35.0%
AGAGGATATGTCTGATAGAA+TGG - chr5.2:3628104-3628123 MS.gene050374:CDS 35.0%
ATTGGATGAAATTGCCAAGT+TGG - chr5.2:3627495-3627514 MS.gene050374:intron 35.0%
ATTTCATAACAGCAGCTTTC+AGG + chr5.2:3626613-3626632 None:intergenic 35.0%
CAAGGAATAAAATTGCAGAG+AGG - chr5.2:3627365-3627384 MS.gene050374:intron 35.0%
CCTGATAATCTTCTATGACA+GGG + chr5.2:3628434-3628453 None:intergenic 35.0%
CTACTATAGAAGAACTTGAG+GGG - chr5.2:3627296-3627315 MS.gene050374:intron 35.0%
GAAAAGAAGGATTGATGTTG+AGG - chr5.2:3627318-3627337 MS.gene050374:intron 35.0%
GATCTCTTTCTAACTGTTTC+TGG + chr5.2:3627436-3627455 None:intergenic 35.0%
TAAAGGAGATGCTATCATGA+TGG - chr5.2:3627654-3627673 MS.gene050374:CDS 35.0%
TAAGATTCCAGTGGTTTCAA+AGG + chr5.2:3628713-3628732 None:intergenic 35.0%
TCAGATTTCCGATCAAGAAT+TGG - chr5.2:3627477-3627496 MS.gene050374:intron 35.0%
TCATACTCATCCATATCAAC+AGG + chr5.2:3626998-3627017 None:intergenic 35.0%
TCCTGATAATCTTCTATGAC+AGG + chr5.2:3628435-3628454 None:intergenic 35.0%
TCGGCGAATTAACGTAAATA+GGG + chr5.2:3628964-3628983 None:intergenic 35.0%
TCTTCAGCATCTTCTTGAAT+TGG + chr5.2:3628066-3628085 None:intergenic 35.0%
TGATAGAATGGCTAGAGAAA+AGG - chr5.2:3628116-3628135 MS.gene050374:CDS 35.0%
TGATAGACCATATGTGTTAC+GGG + chr5.2:3629251-3629270 None:intergenic 35.0%
TGATATGGATGAGTATGAGA+AGG - chr5.2:3627000-3627019 MS.gene050374:intron 35.0%
TGTCATAGAAGATTATCAGG+AGG - chr5.2:3628434-3628453 MS.gene050374:CDS 35.0%
TTCGGCGAATTAACGTAAAT+AGG + chr5.2:3628965-3628984 None:intergenic 35.0%
TTGGATGAAATTGCCAAGTT+GGG - chr5.2:3627496-3627515 MS.gene050374:intron 35.0%
TTTGATACCCGTAACACATA+TGG - chr5.2:3629241-3629260 MS.gene050374:CDS 35.0%
! CATGATGGAAGCTGAAAATT+TGG - chr5.2:3627669-3627688 MS.gene050374:CDS 35.0%
! TGCATCCTTCAGATTTTTCT+GGG - chr5.2:3627743-3627762 MS.gene050374:CDS 35.0%
! TTGCATCCTTCAGATTTTTC+TGG - chr5.2:3627742-3627761 MS.gene050374:CDS 35.0%
!! TTATTTGCTATCTCCTCAAG+CGG + chr5.2:3628366-3628385 None:intergenic 35.0%
AAAGAAAAACCATCCCTTGC+CGG + chr5.2:3627868-3627887 None:intergenic 40.0%
AAATCTGAAGGATGCAACTC+CGG + chr5.2:3627739-3627758 None:intergenic 40.0%
AACCATTAGTGCTACCATTG+TGG - chr5.2:3626820-3626839 MS.gene050374:CDS 40.0%
AACTCCTTGATAAGAGCTGA+CGG - chr5.2:3628240-3628259 MS.gene050374:CDS 40.0%
AAGAACTTGAGGGGAAAAGA+AGG - chr5.2:3627305-3627324 MS.gene050374:intron 40.0%
AAGGACGATAACTATGAACC+TGG - chr5.2:3626902-3626921 MS.gene050374:intron 40.0%
AAGGTTACATTACTCACGCA+AGG - chr5.2:3629376-3629395 MS.gene050374:CDS 40.0%
ACATCAAAATAACCCAGAGG+AGG + chr5.2:3627235-3627254 None:intergenic 40.0%
ACCATTAGTGCTACCATTGT+GGG - chr5.2:3626821-3626840 MS.gene050374:CDS 40.0%
AGATTCCAGTGGTTTCAAAG+GGG + chr5.2:3628711-3628730 None:intergenic 40.0%
AGCTTTCAGGATTTCATCCT+CGG + chr5.2:3626600-3626619 None:intergenic 40.0%
AGGAGTTCTTAAAGGAGTCA+TGG + chr5.2:3627617-3627636 None:intergenic 40.0%
AGGTTACATTACTCACGCAA+GGG - chr5.2:3629377-3629396 MS.gene050374:CDS 40.0%
AGTCCACTCAGTCTTCTTAA+TGG + chr5.2:3626729-3626748 None:intergenic 40.0%
ATAGCATCTCCTTTACTAGC+TGG + chr5.2:3627649-3627668 None:intergenic 40.0%
ATCCTCTTCAATCTTCTCCT+CGG + chr5.2:3628091-3628110 None:intergenic 40.0%
ATGAAAAAAAGTCTGCGCTC+TGG - chr5.2:3627991-3628010 MS.gene050374:CDS 40.0%
CAAAACAGTGTAAAGCTCGT+TGG - chr5.2:3626684-3626703 MS.gene050374:CDS 40.0%
CACTCAGTCTTCTTAATGGA+AGG + chr5.2:3626725-3626744 None:intergenic 40.0%
CAGAAATTCCGTTTGAGAAG+AGG - chr5.2:3627209-3627228 MS.gene050374:intron 40.0%
CCCTGTCATAGAAGATTATC+AGG - chr5.2:3628431-3628450 MS.gene050374:CDS 40.0%
CTCATCCATATCAACAGGAT+CGG + chr5.2:3626993-3627012 None:intergenic 40.0%
CTCCTTTCTTATCATCTCGT+CGG + chr5.2:3628307-3628326 None:intergenic 40.0%
CTTCCATTAAGAAGACTGAG+TGG - chr5.2:3626723-3626742 MS.gene050374:CDS 40.0%
GAATTGGTTGCATCACTATC+TGG + chr5.2:3628050-3628069 None:intergenic 40.0%
GAGCTAGTTGCATATTATGG+TGG - chr5.2:3628793-3628812 MS.gene050374:CDS 40.0%
GCAAATAAGGAGAGAGAGAA+AGG - chr5.2:3628378-3628397 MS.gene050374:CDS 40.0%
GCAACATCAAAATAACCCAG+AGG + chr5.2:3627238-3627257 None:intergenic 40.0%
GCTTGAGGAGATAGCAAATA+AGG - chr5.2:3628365-3628384 MS.gene050374:CDS 40.0%
GTTATCGTCCTTAACACAAG+CGG + chr5.2:3626894-3626913 None:intergenic 40.0%
TAGAGAAAAGGCAGAAGAAG+AGG - chr5.2:3628128-3628147 MS.gene050374:CDS 40.0%
TCATCCATATCAACAGGATC+GGG + chr5.2:3626992-3627011 None:intergenic 40.0%
TCATTCAAAGGGAACTTCCT+AGG - chr5.2:3628187-3628206 MS.gene050374:CDS 40.0%
TGAGAAGTAAGCTGGATGAT+GGG - chr5.2:3629326-3629345 MS.gene050374:CDS 40.0%
TGAGAATGTGAGAAGTAAGC+TGG - chr5.2:3629318-3629337 MS.gene050374:CDS 40.0%
TGATGTTGCTGATGAGGATA+GGG - chr5.2:3627249-3627268 MS.gene050374:intron 40.0%
TGCAGATTTGCAAACGAGTA+GGG + chr5.2:3626666-3626685 None:intergenic 40.0%
TGCTTCAACAAAATCCTCGA+GGG + chr5.2:3629191-3629210 None:intergenic 40.0%
TGGGTGTCTGTGGATAATTA+GGG + chr5.2:3627588-3627607 None:intergenic 40.0%
TGGTTTCAAAGGGGTGAAAA+GGG + chr5.2:3628702-3628721 None:intergenic 40.0%
TTCTTAAAGGAGTCATGGCT+TGG + chr5.2:3627612-3627631 None:intergenic 40.0%
TTGATGTTGCTGATGAGGAT+AGG - chr5.2:3627248-3627267 MS.gene050374:intron 40.0%
TTGCAGATTTGCAAACGAGT+AGG + chr5.2:3626667-3626686 None:intergenic 40.0%
TTGGGTGTCTGTGGATAATT+AGG + chr5.2:3627589-3627608 None:intergenic 40.0%
! GATGATTCGACTTGAGAAGA+AGG - chr5.2:3629357-3629376 MS.gene050374:CDS 40.0%
! GCAAGGGATGGTTTTTCTTT+TGG - chr5.2:3627868-3627887 MS.gene050374:CDS 40.0%
! GCATCCTTCAGATTTTTCTG+GGG - chr5.2:3627744-3627763 MS.gene050374:CDS 40.0%
! GTTCTCTGAGGAGTTCTTAA+AGG + chr5.2:3627625-3627644 None:intergenic 40.0%
! TATGGTCTATCAAGTGTTGC+TGG - chr5.2:3629259-3629278 MS.gene050374:CDS 40.0%
! TGTTCACCTTTTCACCCTTT+GGG - chr5.2:3628555-3628574 MS.gene050374:CDS 40.0%
! TTGTTCACCTTTTCACCCTT+TGG - chr5.2:3628554-3628573 MS.gene050374:CDS 40.0%
!! ACTCATTTTTAGGCTGTGGA+AGG + chr5.2:3628026-3628045 None:intergenic 40.0%
!! TGGTACTCATTTTTAGGCTG+TGG + chr5.2:3628030-3628049 None:intergenic 40.0%
AAAAGGCAGAAGAAGAGGCA+CGG - chr5.2:3628133-3628152 MS.gene050374:CDS 45.0%
AAGAGAGAATTGAAGGCTGC+TGG - chr5.2:3627136-3627155 MS.gene050374:intron 45.0%
AATCACGTTGTGTACCCCAA+AGG + chr5.2:3628573-3628592 None:intergenic 45.0%
AATCTGAAGGATGCAACTCC+GGG + chr5.2:3627738-3627757 None:intergenic 45.0%
AATTTCCTAGGGTCATCACC+AGG + chr5.2:3626923-3626942 None:intergenic 45.0%
ACCCACAATGGTAGCACTAA+TGG + chr5.2:3626825-3626844 None:intergenic 45.0%
AGAGAGAATTGAAGGCTGCT+GGG - chr5.2:3627137-3627156 MS.gene050374:intron 45.0%
AGCATTGTCATGCTCCAGTA+AGG + chr5.2:3628337-3628356 None:intergenic 45.0%
AGCCGACGAGATGATAAGAA+AGG - chr5.2:3628302-3628321 MS.gene050374:CDS 45.0%
AGGCTGCTGGGATTGATATA+AGG - chr5.2:3627149-3627168 MS.gene050374:intron 45.0%
AGGTGTTTGGAAAAACACCG+AGG - chr5.2:3626580-3626599 MS.gene050374:CDS 45.0%
AGTATCTTCGTGAGGCAATG+GGG - chr5.2:3629152-3629171 MS.gene050374:CDS 45.0%
ATAGTGCTAGACTCGAGCTA+CGG - chr5.2:3627956-3627975 MS.gene050374:CDS 45.0%
ATATGTAGCTCATCCCTGAG+AGG + chr5.2:3627913-3627932 None:intergenic 45.0%
ATCACGTTGTGTACCCCAAA+GGG + chr5.2:3628572-3628591 None:intergenic 45.0%
CAATGCTAAGTATCCGCTTG+AGG - chr5.2:3628350-3628369 MS.gene050374:CDS 45.0%
CAGAGAACACCAGCTAGTAA+AGG - chr5.2:3627637-3627656 MS.gene050374:CDS 45.0%
CAGTATCTTCGTGAGGCAAT+GGG - chr5.2:3629151-3629170 MS.gene050374:CDS 45.0%
CATTGCCTCACGAAGATACT+GGG + chr5.2:3629152-3629171 None:intergenic 45.0%
CTAATAAGCTCCAGAGATGC+TGG + chr5.2:3628219-3628238 None:intergenic 45.0%
CTAATGGTTCTCCACTGAGT+AGG + chr5.2:3626794-3626813 None:intergenic 45.0%
CTCAGTGGAGAACCATTAGT+TGG - chr5.2:3626795-3626814 MS.gene050374:CDS 45.0%
CTGGGATTGATATAAGGCAG+CGG - chr5.2:3627155-3627174 MS.gene050374:intron 45.0%
GATCCAAACGAGCAGCAAAT+GGG - chr5.2:3628400-3628419 MS.gene050374:CDS 45.0%
GCATGAAAATGATTCCCTCG+AGG - chr5.2:3629174-3629193 MS.gene050374:CDS 45.0%
GGTTTCAAAGGGGTGAAAAG+GGG + chr5.2:3628701-3628720 None:intergenic 45.0%
GTAAAGCTCGTTGGTATGAG+TGG - chr5.2:3626693-3626712 MS.gene050374:CDS 45.0%
GTGACCCCAGAAAAATCTGA+AGG + chr5.2:3627751-3627770 None:intergenic 45.0%
GTGAGAAGTAAGCTGGATGA+TGG - chr5.2:3629325-3629344 MS.gene050374:CDS 45.0%
GTGCTTCAACAAAATCCTCG+AGG + chr5.2:3629192-3629211 None:intergenic 45.0%
GTGGTTTCAAAGGGGTGAAA+AGG + chr5.2:3628703-3628722 None:intergenic 45.0%
TCCTCTTCAATCTTCTCCTC+GGG + chr5.2:3628090-3628109 None:intergenic 45.0%
TCTTCTATGACAGGGATAGC+AGG + chr5.2:3628426-3628445 None:intergenic 45.0%
TCTTGATCGGAAATCTGAGG+TGG + chr5.2:3627475-3627494 None:intergenic 45.0%
TGAAAATTTGGCCAGGCTGA+GGG - chr5.2:3627681-3627700 MS.gene050374:CDS 45.0%
TGAGAAAGCAAGACGTTGCA+AGG - chr5.2:3627347-3627366 MS.gene050374:intron 45.0%
TGGAAGCTGAAAATTTGGCC+AGG - chr5.2:3627674-3627693 MS.gene050374:CDS 45.0%
TGGGGTCACTCCTAAGAAAA+AGG - chr5.2:3627762-3627781 MS.gene050374:CDS 45.0%
TTACTAGCTGGTGTTCTCTG+AGG + chr5.2:3627637-3627656 None:intergenic 45.0%
TTCTGGGTCATTCAGCTTCT+TGG + chr5.2:3627419-3627438 None:intergenic 45.0%
! GTTCACCTTTTCACCCTTTG+GGG - chr5.2:3628556-3628575 MS.gene050374:CDS 45.0%
! TCTTTTGGCATGACTCCGAA+GGG - chr5.2:3627883-3627902 MS.gene050374:CDS 45.0%
! TTCTTTTGGCATGACTCCGA+AGG - chr5.2:3627882-3627901 MS.gene050374:CDS 45.0%
!! AAAGGAGCTTCTAGCCTTAC+TGG - chr5.2:3628320-3628339 MS.gene050374:CDS 45.0%
!! AAAGGAGTCATGGCTTGGTT+GGG + chr5.2:3627607-3627626 None:intergenic 45.0%
!! AAGTAAGCTGGATGATGGGA+AGG - chr5.2:3629330-3629349 MS.gene050374:CDS 45.0%
!! CAGCATCTCTGGAGCTTATT+AGG - chr5.2:3628217-3628236 MS.gene050374:CDS 45.0%
!! TAAAGGAGTCATGGCTTGGT+TGG + chr5.2:3627608-3627627 None:intergenic 45.0%
!! TTTCACCCCTTTGAAACCAC+TGG - chr5.2:3628703-3628722 MS.gene050374:CDS 45.0%
!! AAAATTAAATAGAAAAAATT+TGG - chr5.2:3628737-3628756 MS.gene050374:CDS 5.0%
ACCCTAGGAAATTGCGTCCA+GGG - chr5.2:3626930-3626949 MS.gene050374:intron 50.0%
ACTGTTAGGAGGAGAGAACC+CGG - chr5.2:3627717-3627736 MS.gene050374:CDS 50.0%
AGAAACAGCTTGAGGAAGCG+AGG - chr5.2:3627092-3627111 MS.gene050374:intron 50.0%
AGAGGAGGCCTCTTCTCAAA+CGG + chr5.2:3627220-3627239 None:intergenic 50.0%
AGCTAGGGAGAAACAGCTTG+AGG - chr5.2:3627084-3627103 MS.gene050374:intron 50.0%
ATAAGCTCCAGAGATGCTGG+AGG + chr5.2:3628216-3628235 None:intergenic 50.0%
CCAGTATCTTCGTGAGGCAA+TGG - chr5.2:3629150-3629169 MS.gene050374:CDS 50.0%
CCATTGCCTCACGAAGATAC+TGG + chr5.2:3629153-3629172 None:intergenic 50.0%
CCTTACTCCCAGAAGTGGTA+TGG - chr5.2:3627840-3627859 MS.gene050374:CDS 50.0%
CTAAGCTTACGCCTACTCAG+TGG - chr5.2:3626780-3626799 MS.gene050374:CDS 50.0%
CTCCTTCGCAGTGTCTTGAA+CGG - chr5.2:3626849-3626868 MS.gene050374:CDS 50.0%
CTGAAAATTTGGCCAGGCTG+AGG - chr5.2:3627680-3627699 MS.gene050374:CDS 50.0%
GAGTCTCAGACACCACTGTT+AGG - chr5.2:3627703-3627722 MS.gene050374:CDS 50.0%
GCAGGCTTCGATTCAGGATT+CGG + chr5.2:3626965-3626984 None:intergenic 50.0%
GGATCCAAACGAGCAGCAAA+TGG - chr5.2:3628399-3628418 MS.gene050374:CDS 50.0%
GGATTGATATAAGGCAGCGG+AGG - chr5.2:3627158-3627177 MS.gene050374:intron 50.0%
GGTTATGCCATGGACCTTGT+GGG - chr5.2:3627517-3627536 MS.gene050374:intron 50.0%
GTCCATGGCATAACCCAACT+TGG + chr5.2:3627512-3627531 None:intergenic 50.0%
GTCTCCGTCAGCTCTTATCA+AGG + chr5.2:3628247-3628266 None:intergenic 50.0%
GTGTACCCCAAAGGGTGAAA+AGG + chr5.2:3628564-3628583 None:intergenic 50.0%
TACCGTTCAAGACACTGCGA+AGG + chr5.2:3626854-3626873 None:intergenic 50.0%
TCTCAGACACCACTGTTAGG+AGG - chr5.2:3627706-3627725 MS.gene050374:CDS 50.0%
TGAAGCCCAGTATCTTCGTG+AGG - chr5.2:3629144-3629163 MS.gene050374:CDS 50.0%
TGCTTTCCGAAGCATGTGCT+CGG - chr5.2:3627026-3627045 MS.gene050374:intron 50.0%
TGTTGCTGATGAGGATAGGG+AGG - chr5.2:3627252-3627271 MS.gene050374:intron 50.0%
TTGATATAAGGCAGCGGAGG+AGG - chr5.2:3627161-3627180 MS.gene050374:intron 50.0%
TTGGCATGCAGAATAGCAGC+CGG + chr5.2:3627400-3627419 None:intergenic 50.0%
TTTGAGAAGAGGCCTCCTCT+GGG - chr5.2:3627220-3627239 MS.gene050374:intron 50.0%
! ATGAACCTGGTGATGACCCT+AGG - chr5.2:3626915-3626934 MS.gene050374:intron 50.0%
! CCATACCACTTCTGGGAGTA+AGG + chr5.2:3627843-3627862 None:intergenic 50.0%
! TGATCGGAAATCTGAGGTGG+TGG + chr5.2:3627472-3627491 None:intergenic 50.0%
! TGCCAAGTTGGGTTATGCCA+TGG - chr5.2:3627507-3627526 MS.gene050374:intron 50.0%
!! ATCTCGTCGGCTTGCTCAAT+AGG + chr5.2:3628294-3628313 None:intergenic 50.0%
!! TCTCGTCGGCTTGCTCAATA+GGG + chr5.2:3628293-3628312 None:intergenic 50.0%
AACAGCTTGAGGAAGCGAGG+AGG - chr5.2:3627095-3627114 MS.gene050374:intron 55.0%
ACGAGTAGCACTGCTGCCTT+CGG + chr5.2:3627557-3627576 None:intergenic 55.0%
AGAAGATGCTGAAGAGCCCG+AGG - chr5.2:3628071-3628090 MS.gene050374:CDS 55.0%
AGCCAACCGAGCACATGCTT+CGG + chr5.2:3627035-3627054 None:intergenic 55.0%
AGCGGAGGAGGAAAAGGAGA+GGG - chr5.2:3627173-3627192 MS.gene050374:intron 55.0%
AGTAAGGCCTGCACTTCCAG+GGG + chr5.2:3627827-3627846 None:intergenic 55.0%
CCCCTGGACGCAATTTCCTA+GGG + chr5.2:3626934-3626953 None:intergenic 55.0%
CCCTAGGAAATTGCGTCCAG+GGG - chr5.2:3626931-3626950 MS.gene050374:intron 55.0%
CCTTAACACAAGCGGCGTCA+AGG + chr5.2:3626886-3626905 None:intergenic 55.0%
CCTTGACGCCGCTTGTGTTA+AGG - chr5.2:3626883-3626902 MS.gene050374:intron 55.0%
GACCCTAGGAAATTGCGTCC+AGG - chr5.2:3626929-3626948 MS.gene050374:intron 55.0%
GAGTAAGGCCTGCACTTCCA+GGG + chr5.2:3627828-3627847 None:intergenic 55.0%
GCCCGAGGAGAAGATTGAAG+AGG - chr5.2:3628086-3628105 MS.gene050374:CDS 55.0%
GCGACCCGATCCTGTTGATA+TGG - chr5.2:3626985-3627004 MS.gene050374:intron 55.0%
GCTTGCTCAATAGGGGTTGG+TGG + chr5.2:3628285-3628304 None:intergenic 55.0%
GGATTCGGATCAATCTCCCC+TGG + chr5.2:3626950-3626969 None:intergenic 55.0%
GGGTTATGCCATGGACCTTG+TGG - chr5.2:3627516-3627535 MS.gene050374:intron 55.0%
GGGTTCTCTCCTCCTAACAG+TGG + chr5.2:3627718-3627737 None:intergenic 55.0%
GTTTGAGAAGAGGCCTCCTC+TGG - chr5.2:3627219-3627238 MS.gene050374:intron 55.0%
TAAGGCAGCGGAGGAGGAAA+AGG - chr5.2:3627167-3627186 MS.gene050374:intron 55.0%
TCCCCTGGACGCAATTTCCT+AGG + chr5.2:3626935-3626954 None:intergenic 55.0%
TCCCTGAGAGGAGTTCCCTT+CGG + chr5.2:3627901-3627920 None:intergenic 55.0%
TCCGAAGGGAACTCCTCTCA+GGG - chr5.2:3627897-3627916 MS.gene050374:CDS 55.0%
TGAGTTCATCGCTGCCCACA+AGG + chr5.2:3627534-3627553 None:intergenic 55.0%
TGCTGATGAGGATAGGGAGG+TGG - chr5.2:3627255-3627274 MS.gene050374:intron 55.0%
TGGCATGCAGAATAGCAGCC+GGG + chr5.2:3627399-3627418 None:intergenic 55.0%
TTCCGAAGCATGTGCTCGGT+TGG - chr5.2:3627030-3627049 MS.gene050374:intron 55.0%
! AGAAGAAGAGGCACGGCAGA+AGG - chr5.2:3628140-3628159 MS.gene050374:CDS 55.0%
! AGGCCCATTTGCTGCTCGTT+TGG + chr5.2:3628406-3628425 None:intergenic 55.0%
! CGAAGGAGTACGACCCACAA+TGG + chr5.2:3626837-3626856 None:intergenic 55.0%
! GACTGAGTGGACTCGAGAAG+AGG - chr5.2:3626736-3626755 MS.gene050374:CDS 55.0%
! GCTCGGTTGGCTAACACGAA+AGG - chr5.2:3627043-3627062 MS.gene050374:intron 55.0%
! TCGGCTTGCTCAATAGGGGT+TGG + chr5.2:3628288-3628307 None:intergenic 55.0%
!! ATGGCTTGGTTGGGTGTCTG+TGG + chr5.2:3627598-3627617 None:intergenic 55.0%
!! CTCGTCGGCTTGCTCAATAG+GGG + chr5.2:3628292-3628311 None:intergenic 55.0%
AGAGATGCTGGAGGAGGCCT+AGG + chr5.2:3628207-3628226 None:intergenic 60.0%
AGCTCCAGAGATGCTGGAGG+AGG + chr5.2:3628213-3628232 None:intergenic 60.0%
ATCGCTGCCCACAAGGTCCA+TGG + chr5.2:3627527-3627546 None:intergenic 60.0%
CAGCGGAGGAGGAAAAGGAG+AGG - chr5.2:3627172-3627191 MS.gene050374:intron 60.0%
CTCCGAAGGGAACTCCTCTC+AGG - chr5.2:3627896-3627915 MS.gene050374:CDS 60.0%
GCACTTCCAGGGGTTGCAGA+GGG + chr5.2:3627817-3627836 None:intergenic 60.0%
GCAGGCCTTACTCCCAGAAG+TGG - chr5.2:3627835-3627854 MS.gene050374:CDS 60.0%
GGAGTAAGGCCTGCACTTCC+AGG + chr5.2:3627829-3627848 None:intergenic 60.0%
GGCAGCGATGAACTCACCGA+AGG - chr5.2:3627538-3627557 MS.gene050374:CDS 60.0%
GGCATGCAGAATAGCAGCCG+GGG + chr5.2:3627398-3627417 None:intergenic 60.0%
TAGGCCTCCTCCAGCATCTC+TGG - chr5.2:3628206-3628225 MS.gene050374:CDS 60.0%
TCCCAGAAGTGGTATGGCGC+CGG - chr5.2:3627846-3627865 MS.gene050374:CDS 60.0%
TGCACTTCCAGGGGTTGCAG+AGG + chr5.2:3627818-3627837 None:intergenic 60.0%
TGCAGAGAGGCAAGATGCCC+CGG - chr5.2:3627378-3627397 MS.gene050374:intron 60.0%
TTGCAGAGGGAGTCGGCAGT+GGG + chr5.2:3627804-3627823 None:intergenic 60.0%
! TCTGCAACCCCTGGAAGTGC+AGG - chr5.2:3627817-3627836 MS.gene050374:CDS 60.0%
! TGTCTGAGACTCCCTCAGCC+TGG + chr5.2:3627695-3627714 None:intergenic 60.0%
!! AAGTGGTATGGCGCCGGCAA+GGG - chr5.2:3627852-3627871 MS.gene050374:CDS 60.0%
CCAGGGGTTGCAGAGGGAGT+CGG + chr5.2:3627811-3627830 None:intergenic 65.0%
GAGGGAGTCGGCAGTGGGAT+TGG + chr5.2:3627799-3627818 None:intergenic 65.0%
GCCGGCGCCATACCACTTCT+GGG + chr5.2:3627850-3627869 None:intergenic 65.0%
GGTCGCGCAGGCTTCGATTC+AGG + chr5.2:3626971-3626990 None:intergenic 65.0%
GTTGCAGAGGGAGTCGGCAG+TGG + chr5.2:3627805-3627824 None:intergenic 65.0%
TCAACAGGATCGGGTCGCGC+AGG + chr5.2:3626983-3627002 None:intergenic 65.0%
TGCCGGCGCCATACCACTTC+TGG + chr5.2:3627851-3627870 None:intergenic 65.0%
!! GAAGTGGTATGGCGCCGGCA+AGG - chr5.2:3627851-3627870 MS.gene050374:CDS 65.0%
CCGACTCCCTCTGCAACCCC+TGG - chr5.2:3627808-3627827 MS.gene050374:CDS 70.0%
!! GGTATGGCGCCGGCAAGGGA+TGG - chr5.2:3627856-3627875 MS.gene050374:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 3626572 3629420 3626572 ID=MS.gene050374
chr5.2 mRNA 3626572 3629420 3626572 ID=MS.gene050374.t1;Parent=MS.gene050374
chr5.2 exon 3627522 3629420 3627522 ID=MS.gene050374.t1.exon1;Parent=MS.gene050374.t1
chr5.2 CDS 3627522 3629420 3627522 ID=cds.MS.gene050374.t1;Parent=MS.gene050374.t1
chr5.2 exon 3626572 3626868 3626572 ID=MS.gene050374.t1.exon2;Parent=MS.gene050374.t1
chr5.2 CDS 3626572 3626868 3626572 ID=cds.MS.gene050374.t1;Parent=MS.gene050374.t1
Gene Sequence

>MS.gene050374

ATGAGCATAGGTGTTTGGAAAAACACCGAGGATGAAATCCTGAAAGCTGCTGTTATGAAATATGGTAAAAATCAGTGGGCTCATATCTCGTCCCTACTCGTTTGCAAATCTGCAAAACAGTGTAAAGCTCGTTGGTATGAGTGGTTAGATCCTTCCATTAAGAAGACTGAGTGGACTCGAGAAGAGGATGAGAAGCTACTTCATCTTGCTAAGCTTACGCCTACTCAGTGGAGAACCATTAGTTGGAGAACCATTAGTGCTACCATTGTGGGTCGTACTCCTTCGCAGTGTCTTGAACGGTATGAGAAACTCCTTGACGCCGCTTGTGTTAAGGACGATAACTATGAACCTGGTGATGACCCTAGGAAATTGCGTCCAGGGGAGATTGATCCGAATCCTGAATCGAAGCCTGCGCGACCCGATCCTGTTGATATGGATGAGTATGAGAAGGAGATGCTTTCCGAAGCATGTGCTCGGTTGGCTAACACGAAAGGTAAAAAAGCAAAAAGGAAAGCTAGGGAGAAACAGCTTGAGGAAGCGAGGAGGCTTGCTTCTTTACAGAAAAAGAGAGAATTGAAGGCTGCTGGGATTGATATAAGGCAGCGGAGGAGGAAAAGGAGAGGGATTGATTATAATGCAGAAATTCCGTTTGAGAAGAGGCCTCCTCTGGGTTATTTTGATGTTGCTGATGAGGATAGGGAGGTGGAGCAGCATGCATTCCCTACTACTATAGAAGAACTTGAGGGGAAAAGAAGGATTGATGTTGAGGCTCAGTTGAGAAAGCAAGACGTTGCAAGGAATAAAATTGCAGAGAGGCAAGATGCCCCGGCTGCTATTCTGCATGCCAAGAAGCTGAATGACCCAGAAACAGTTAGAAAGAGATCAAAATTGATGCTTCCACCACCTCAGATTTCCGATCAAGAATTGGATGAAATTGCCAAGTTGGGTTATGCCATGGACCTTGTGGGCAGCGATGAACTCACCGAAGGCAGCAGTGCTACTCGTGCTCTTCTCCCTAATTATCCACAGACACCCAACCAAGCCATGACTCCTTTAAGAACTCCTCAGAGAACACCAGCTAGTAAAGGAGATGCTATCATGATGGAAGCTGAAAATTTGGCCAGGCTGAGGGAGTCTCAGACACCACTGTTAGGAGGAGAGAACCCGGAGTTGCATCCTTCAGATTTTTCTGGGGTCACTCCTAAGAAAAAGGAAATTCACATTCCAATCCCACTGCCGACTCCCTCTGCAACCCCTGGAAGTGCAGGCCTTACTCCCAGAAGTGGTATGGCGCCGGCAAGGGATGGTTTTTCTTTTGGCATGACTCCGAAGGGAACTCCTCTCAGGGATGAGCTACATATCAATGAAGATATGGAGATGCGTGATAGTGCTAGACTCGAGCTACGGAGACAAGCTGATATGAAAAAAAGTCTGCGCTCTGGTTTAAGCAGCCTTCCACAGCCTAAAAATGAGTACCAGATAGTGATGCAACCAATTCAAGAAGATGCTGAAGAGCCCGAGGAGAAGATTGAAGAGGATATGTCTGATAGAATGGCTAGAGAAAAGGCAGAAGAAGAGGCACGGCAGAAGGCATTACTTAGAAAACGATCAAAAGTCATTCAAAGGGAACTTCCTAGGCCTCCTCCAGCATCTCTGGAGCTTATTAGGAACTCCTTGATAAGAGCTGACGGAGACAAAAGTTCATTTGTTCCACCAACCCCTATTGAGCAAGCCGACGAGATGATAAGAAAGGAGCTTCTAGCCTTACTGGAGCATGACAATGCTAAGTATCCGCTTGAGGAGATAGCAAATAAGGAGAGAGAGAAAGGATCCAAACGAGCAGCAAATGGGCCTGCTATCCCTGTCATAGAAGATTATCAGGAGGACGAGATGAAATCTGTATGTTTTTACTAATTTATCTGCTCACCTTTAATTTTATTTTTGTCTTTTAATTCGCTTTTCTTCATTTGCTACTAAGTAGCTTGTTCACCTTTTCACCCTTTGGGGTACACAACGTGATTTTTCATTTAGATGATACACATGCTTTTTCTGATGATCTTGACCTTGAAATTATTAGTACTTTGTCTGATTATTAATTACTTGGCATCATCATTTTTATGCTGAAACCCCTTTTCACCCCTTTGAAACCACTGGAATCTTATGTAAAAATTAAATAGAAAAAATTTGGACATCTTTTTAATTTATGTATCATCTCCTTTATGAGCTAGTTGCATATTATGGTGGTGTATTGTATCGTCCTTGTTTTTTTTCAGTTATAATCATTTGATTAATTAGGTTGAAGTTAGTTTTAGTTTCTCGCAATTGTTGTTCTCTATTTCTTTCGTTTTTGTAACAAGTGATTCTTCAATTGCCTTTTACAACATTTATTCCCTATTTACGTTAATTCGCCGAATGATAGTAATACAAACTTCCAAACTGCTTGTTAATTATTATTTGACTCCTTAAATTTGACTATGGAGATGAGCTTGTACGTTGATGAATGATAATATCTGTGTTGACTGTTATTTATTTTTATCTGTTGTTCTCAATAGGCTGATCAGTTGATAAAAGATGAAGCCCAGTATCTTCGTGAGGCAATGGGGCATGAAAATGATTCCCTCGAGGATTTTGTTGAAGCACATACAACATGCATTAATGATTTAATGTACTTTGATACCCGTAACACATATGGTCTATCAAGTGTTGCTGGAAACGCAGAGAAGCTAGCAGCTTTGCAGAATGAATTTGAGAATGTGAGAAGTAAGCTGGATGATGGGAAGGAGAAGATGATTCGACTTGAGAAGAAGGTTACATTACTCACGCAAGGGTACGAGTTATGCTACTGCTAA
Protein sequence

>MS.gene050374.t1

MSIGVWKNTEDEILKAAVMKYGKNQWAHISSLLVCKSAKQCKARWYEWLDPSIKKTEWTREEDEKLLHLAKLTPTQWRTISWRTISATIVGRTPSQCLERYEKLLDAACVKDDNYEPGDDPRKLRPGEIDPNPESKPARPDPVDMDEYEKEMLSEACARLANTKGKKAKRKAREKQLEEARRLASLQKKRELKAAGIDIRQRRRKRRGIDYNAEIPFEKRPPLGYFDVADEDREVEQHAFPTTIEELEGKRRIDVEAQLRKQDVARNKIAERQDAPAAILHAKKLNDPETVRKRSKLMLPPPQISDQELDEIAKLGYAMDLVGSDELTEGSSATRALLPNYPQTPNQAMTPLRTPQRTPASKGDAIMMEAENLARLRESQTPLLGGENPELHPSDFSGVTPKKKEIHIPIPLPTPSATPGSAGLTPRSGMAPARDGFSFGMTPKGTPLRDELHINEDMEMRDSARLELRRQADMKKSLRSGLSSLPQPKNEYQIVMQPIQEDAEEPEEKIEEDMSDRMAREKAEEEARQKALLRKRSKVIQRELPRPPPASLELIRNSLIRADGDKSSFVPPTPIEQADEMIRKELLALLEHDNAKYPLEEIANKEREKGSKRAANGPAIPVIEDYQEDEMKSADQLIKDEAQYLREAMGHENDSLEDFVEAHTTCINDLMYFDTRNTYGLSSVAGNAEKLAALQNEFENVRSKLDDGKEKMIRLEKKVTLLTQGYELCYC