Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94257.t1 | XP_003623166.1 | 98.3 | 117 | 2 | 0 | 1 | 117 | 1 | 117 | 3.10E-58 | 234.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94257.t1 | G7L5L7 | 98.3 | 117 | 2 | 0 | 1 | 117 | 1 | 117 | 2.2e-58 | 234.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050710 | MS.gene94257 | 0.820972 | 5.17E-53 | -1.69E-46 |
| MS.gene051552 | MS.gene94257 | 0.824211 | 9.13E-54 | -1.69E-46 |
| MS.gene052606 | MS.gene94257 | 0.810438 | 1.15E-50 | -1.69E-46 |
| MS.gene053108 | MS.gene94257 | 0.812071 | 5.10E-51 | -1.69E-46 |
| MS.gene055157 | MS.gene94257 | 0.856937 | 2.30E-62 | -1.69E-46 |
| MS.gene055158 | MS.gene94257 | 0.885998 | 5.01E-72 | -1.69E-46 |
| MS.gene055159 | MS.gene94257 | 0.863721 | 2.03E-64 | -1.69E-46 |
| MS.gene056678 | MS.gene94257 | 0.812097 | 5.03E-51 | -1.69E-46 |
| MS.gene058158 | MS.gene94257 | 0.821409 | 4.10E-53 | -1.69E-46 |
| MS.gene058418 | MS.gene94257 | 0.825018 | 5.89E-54 | -1.69E-46 |
| MS.gene05926 | MS.gene94257 | 0.809364 | 1.96E-50 | -1.69E-46 |
| MS.gene05960 | MS.gene94257 | 0.813058 | 3.10E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94257.t1 | MTR_7g065210 | 98.291 | 117 | 2 | 0 | 1 | 117 | 1 | 117 | 1.44e-81 | 234 |
| MS.gene94257.t1 | MTR_7g065160 | 88.034 | 117 | 14 | 0 | 1 | 117 | 1 | 117 | 2.92e-74 | 216 |
| MS.gene94257.t1 | MTR_8g070040 | 52.632 | 95 | 43 | 2 | 24 | 117 | 27 | 120 | 1.63e-26 | 95.5 |
| MS.gene94257.t1 | MTR_8g070015 | 48.864 | 88 | 43 | 2 | 24 | 110 | 27 | 113 | 7.94e-21 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94257.t1 | AT3G53980 | 70.526 | 95 | 27 | 1 | 23 | 117 | 21 | 114 | 1.17e-45 | 144 |
| MS.gene94257.t1 | AT3G53980 | 70.526 | 95 | 27 | 1 | 23 | 117 | 21 | 114 | 1.17e-45 | 144 |
| MS.gene94257.t1 | AT5G05960 | 59.649 | 114 | 39 | 2 | 4 | 117 | 10 | 116 | 2.64e-43 | 137 |
| MS.gene94257.t1 | AT2G37870 | 46.789 | 109 | 54 | 3 | 11 | 117 | 9 | 115 | 1.01e-26 | 95.9 |
Find 21 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTAAAAGCTTCATTATC+TGG | 0.286546 | 7.3:-40708233 | None:intergenic |
| TAGCAAAGTTGCAGCGTTTA+GGG | 0.296288 | 7.3:-40708418 | None:intergenic |
| GTCCTTGTTGCAGGGATTCA+TGG | 0.338655 | 7.3:+40708173 | MS.gene94257:CDS |
| TTAGCAAAGTTGCAGCGTTT+AGG | 0.351023 | 7.3:-40708419 | None:intergenic |
| CGTCCGCACTTATAACCAAC+GGG | 0.452259 | 7.3:-40708446 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.462464 | 7.3:+40708368 | MS.gene94257:CDS |
| GGATAGTAACAGCAATTTGA+GGG | 0.472152 | 7.3:-40708397 | None:intergenic |
| ATGAAGATGCTGTGCCTAGT+TGG | 0.489536 | 7.3:+40708143 | MS.gene94257:CDS |
| GGGATAGTAACAGCAATTTG+AGG | 0.508534 | 7.3:-40708398 | None:intergenic |
| GTTGAAAGTGCTGGTGAATG+TGG | 0.534504 | 7.3:+40708197 | MS.gene94257:CDS |
| TCTTGTGCTGCACTTGCACA+AGG | 0.541464 | 7.3:-40708260 | None:intergenic |
| AACTTTGCTAATCGCCCCGT+TGG | 0.551232 | 7.3:+40708431 | MS.gene94257:CDS |
| CAAGGACTACAAAACCAACT+AGG | 0.588924 | 7.3:-40708157 | None:intergenic |
| AAAACTGCACAAAGACAACT+TGG | 0.590774 | 7.3:-40708341 | None:intergenic |
| TGTGCTCAGACTAAGAAACT+TGG | 0.591888 | 7.3:+40708311 | MS.gene94257:CDS |
| CTCCATGAATCCCTGCAACA+AGG | 0.594877 | 7.3:-40708175 | None:intergenic |
| ACGTCCGCACTTATAACCAA+CGG | 0.607634 | 7.3:-40708447 | None:intergenic |
| AGTGCTGGTGAATGTGGAAG+AGG | 0.608175 | 7.3:+40708203 | MS.gene94257:CDS |
| ATTCATGGAGTTGAAAGTGC+TGG | 0.616969 | 7.3:+40708188 | MS.gene94257:CDS |
| CGCCCCGTTGGTTATAAGTG+CGG | 0.662870 | 7.3:+40708443 | MS.gene94257:CDS |
| GTCCGCACTTATAACCAACG+GGG | 0.801503 | 7.3:-40708445 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAGATAGTTAAGAGTATTAT+AGG | + | chr7.3:40708661-40708680 | MS.gene94257:intron | 20.0% |
| ! | ACAACGAAAAAAATGTGAAA+CGG | + | chr7.3:40708709-40708728 | MS.gene94257:intron | 25.0% |
| ! | TATCTGCAATAGATAGTTAA+AGG | - | chr7.3:40708618-40708637 | None:intergenic | 25.0% |
| ! | TTTATACGAAAACAAACTGA+GGG | - | chr7.3:40708488-40708507 | None:intergenic | 25.0% |
| !! | CTATTTGTCAATTTTCTTGA+TGG | + | chr7.3:40708773-40708792 | MS.gene94257:intron | 25.0% |
| !! | TTTTATACGAAAACAAACTG+AGG | - | chr7.3:40708489-40708508 | None:intergenic | 25.0% |
| AGCTTAAAAGCTTCATTATC+TGG | - | chr7.3:40708236-40708255 | None:intergenic | 30.0% | |
| AAAACTGCACAAAGACAACT+TGG | - | chr7.3:40708344-40708363 | None:intergenic | 35.0% | |
| GGATAGTAACAGCAATTTGA+GGG | - | chr7.3:40708400-40708419 | None:intergenic | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | + | chr7.3:40708368-40708387 | MS.gene94257:CDS | 35.0% | |
| CAAGGACTACAAAACCAACT+AGG | - | chr7.3:40708160-40708179 | None:intergenic | 40.0% | |
| GGGATAGTAACAGCAATTTG+AGG | - | chr7.3:40708401-40708420 | None:intergenic | 40.0% | |
| TAGCAAAGTTGCAGCGTTTA+GGG | - | chr7.3:40708421-40708440 | None:intergenic | 40.0% | |
| TGTGCTCAGACTAAGAAACT+TGG | + | chr7.3:40708311-40708330 | MS.gene94257:CDS | 40.0% | |
| TTAGCAAAGTTGCAGCGTTT+AGG | - | chr7.3:40708422-40708441 | None:intergenic | 40.0% | |
| ! | ATTCATGGAGTTGAAAGTGC+TGG | + | chr7.3:40708188-40708207 | MS.gene94257:CDS | 40.0% |
| ! | GTTTTGTAGTCCTTGTTGCA+GGG | + | chr7.3:40708165-40708184 | MS.gene94257:CDS | 40.0% |
| ACGTCCGCACTTATAACCAA+CGG | - | chr7.3:40708450-40708469 | None:intergenic | 45.0% | |
| ATGAAGATGCTGTGCCTAGT+TGG | + | chr7.3:40708143-40708162 | MS.gene94257:CDS | 45.0% | |
| !! | GGTTTTGTAGTCCTTGTTGC+AGG | + | chr7.3:40708164-40708183 | MS.gene94257:CDS | 45.0% |
| !! | GTTGAAAGTGCTGGTGAATG+TGG | + | chr7.3:40708197-40708216 | MS.gene94257:CDS | 45.0% |
| AACTTTGCTAATCGCCCCGT+TGG | + | chr7.3:40708431-40708450 | MS.gene94257:CDS | 50.0% | |
| CGTCCGCACTTATAACCAAC+GGG | - | chr7.3:40708449-40708468 | None:intergenic | 50.0% | |
| CTCCATGAATCCCTGCAACA+AGG | - | chr7.3:40708178-40708197 | None:intergenic | 50.0% | |
| GTCCGCACTTATAACCAACG+GGG | - | chr7.3:40708448-40708467 | None:intergenic | 50.0% | |
| GTCCTTGTTGCAGGGATTCA+TGG | + | chr7.3:40708173-40708192 | MS.gene94257:CDS | 50.0% | |
| TCTTGTGCTGCACTTGCACA+AGG | - | chr7.3:40708263-40708282 | None:intergenic | 50.0% | |
| !! | AGTGCTGGTGAATGTGGAAG+AGG | + | chr7.3:40708203-40708222 | MS.gene94257:CDS | 50.0% |
| ! | CGCCCCGTTGGTTATAAGTG+CGG | + | chr7.3:40708443-40708462 | MS.gene94257:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 40708131 | 40708853 | 40708131 | ID=MS.gene94257 |
| chr7.3 | mRNA | 40708131 | 40708853 | 40708131 | ID=MS.gene94257.t1;Parent=MS.gene94257 |
| chr7.3 | exon | 40708131 | 40708467 | 40708131 | ID=MS.gene94257.t1.exon1;Parent=MS.gene94257.t1 |
| chr7.3 | CDS | 40708131 | 40708467 | 40708131 | ID=cds.MS.gene94257.t1;Parent=MS.gene94257.t1 |
| chr7.3 | exon | 40708837 | 40708853 | 40708837 | ID=MS.gene94257.t1.exon2;Parent=MS.gene94257.t1 |
| chr7.3 | CDS | 40708837 | 40708853 | 40708837 | ID=cds.MS.gene94257.t1;Parent=MS.gene94257.t1 |
| Gene Sequence |
| Protein sequence |
