Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95676.t1 | KEH44502.1 | 64 | 139 | 25 | 2 | 12 | 125 | 4 | 142 | 3.80E-36 | 161 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95676.t1 | A0A072VRV0 | 64.0 | 139 | 25 | 2 | 12 | 125 | 4 | 142 | 2.8e-36 | 161.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049293 | MS.gene95676 | -0.815617 | 8.43E-52 | -1.69E-46 |
| MS.gene053963 | MS.gene95676 | 0.810517 | 1.11E-50 | -1.69E-46 |
| MS.gene054222 | MS.gene95676 | 0.810972 | 8.83E-51 | -1.69E-46 |
| MS.gene05548 | MS.gene95676 | 0.807747 | 4.35E-50 | -1.69E-46 |
| MS.gene056068 | MS.gene95676 | 0.802086 | 6.63E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene95676 | -0.810331 | 1.22E-50 | -1.69E-46 |
| MS.gene060365 | MS.gene95676 | 0.8074 | 5.15E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95676.t1 | MTR_1g115520 | 64.029 | 139 | 25 | 2 | 12 | 125 | 4 | 142 | 4.08e-54 | 167 |
| MS.gene95676.t1 | MTR_7g051400 | 34.731 | 167 | 52 | 3 | 1 | 110 | 253 | 419 | 4.74e-23 | 93.6 |
| MS.gene95676.t1 | MTR_1g105785 | 33.533 | 167 | 54 | 3 | 1 | 110 | 275 | 441 | 5.98e-21 | 87.8 |
| MS.gene95676.t1 | MTR_7g110100 | 29.762 | 168 | 59 | 3 | 1 | 109 | 275 | 442 | 8.59e-18 | 78.6 |
| MS.gene95676.t1 | MTR_1g492690 | 28.387 | 155 | 63 | 1 | 1 | 107 | 78 | 232 | 2.29e-17 | 77.0 |
| MS.gene95676.t1 | MTR_4g032565 | 30.682 | 176 | 59 | 5 | 1 | 115 | 19 | 192 | 2.60e-17 | 76.3 |
| MS.gene95676.t1 | MTR_4g032585 | 29.885 | 174 | 63 | 3 | 1 | 115 | 19 | 192 | 2.66e-17 | 76.3 |
| MS.gene95676.t1 | MTR_4g060433 | 70.833 | 48 | 14 | 0 | 1 | 48 | 19 | 66 | 6.02e-16 | 71.2 |
| MS.gene95676.t1 | MTR_3g452170 | 34.862 | 109 | 57 | 2 | 1 | 109 | 275 | 369 | 9.68e-15 | 69.7 |
| MS.gene95676.t1 | MTR_8g104590 | 58.333 | 48 | 20 | 0 | 1 | 48 | 8 | 55 | 1.00e-13 | 65.1 |
| MS.gene95676.t1 | MTR_2g040680 | 32.710 | 107 | 45 | 2 | 1 | 104 | 93 | 175 | 7.78e-12 | 60.8 |
| MS.gene95676.t1 | MTR_3g046650 | 35.484 | 93 | 34 | 2 | 1 | 67 | 110 | 202 | 1.07e-11 | 60.5 |
| MS.gene95676.t1 | MTR_8g064300 | 40.000 | 65 | 39 | 0 | 45 | 109 | 163 | 227 | 3.15e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAGTGAATCTGATGTTCT+TGG | 0.373509 | 7.4:+33793367 | MS.gene95676:CDS |
| TATAAGCAAGCATAACCTTA+AGG | 0.380702 | 7.4:+33793256 | MS.gene95676:CDS |
| TTTGGTTTGCAGGCATGTCT+TGG | 0.387416 | 7.4:+33792649 | MS.gene95676:intron |
| TCTGTGTTGTTAGGAAGGAT+TGG | 0.428486 | 7.4:+33793509 | MS.gene95676:intron |
| GACAATTGAAGATTTGATTG+AGG | 0.465748 | 7.4:+33792546 | MS.gene95676:CDS |
| AGACAAACAGACGTTGTTCA+AGG | 0.500909 | 7.4:+33793145 | MS.gene95676:CDS |
| TAGGGAATGTGAATTATAAG+TGG | 0.530274 | 7.4:+33793180 | MS.gene95676:CDS |
| ACAGACGTTGTTCAAGGTGG+AGG | 0.574823 | 7.4:+33793151 | MS.gene95676:CDS |
| ACAATTTGCGAGATAGAGAC+TGG | 0.578498 | 7.4:+33792685 | MS.gene95676:CDS |
| TTCAAGGTGGAGGTTAGCAT+AGG | 0.581457 | 7.4:+33793161 | MS.gene95676:CDS |
| TCAAGGTGGAGGTTAGCATA+GGG | 0.582104 | 7.4:+33793162 | MS.gene95676:CDS |
| AGTCTCTATCTCGCAAATTG+TGG | 0.586282 | 7.4:-33792683 | None:intergenic |
| AGATAGAGACTGGTGTCGAA+TGG | 0.611331 | 7.4:+33792695 | MS.gene95676:CDS |
| AGTGAATCTGATGTTCTTGG+TGG | 0.615328 | 7.4:+33793370 | MS.gene95676:CDS |
| AGTCAAGAAAGCTACAACTG+AGG | 0.625317 | 7.4:+33793214 | MS.gene95676:CDS |
| GGATTGCAGGCACATAGTCA+AGG | 0.626684 | 7.4:+33793092 | MS.gene95676:intron |
| GGAATGTGAATTATAAGTGG+AGG | 0.629082 | 7.4:+33793183 | MS.gene95676:CDS |
| GATGACTCAATATTTAATGG+TGG | 0.652456 | 7.4:-33793542 | None:intergenic |
| CAAACAGACGTTGTTCAAGG+TGG | 0.667936 | 7.4:+33793148 | MS.gene95676:CDS |
| ATGCACGTCATTAGTGACTG+TGG | 0.672817 | 7.4:+33792738 | MS.gene95676:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAACTATGATACAAACAAT+TGG | - | chr7.4:33792618-33792637 | None:intergenic | 20.0% |
| !! | CCACTTATAATATAATGAAT+GGG | + | chr7.4:33793439-33793458 | MS.gene95676:intron | 20.0% |
| !! | CCATTCATTATATTATAAGT+GGG | - | chr7.4:33793441-33793460 | None:intergenic | 20.0% |
| !! | TAATATAATGAATGGGTATA+AGG | + | chr7.4:33793446-33793465 | MS.gene95676:intron | 20.0% |
| !!! | AATCTTTTTCAAAATGACAT+GGG | + | chr7.4:33793039-33793058 | MS.gene95676:intron | 20.0% |
| !!! | AATTAAACGAAATTTTTTGC+AGG | + | chr7.4:33793071-33793090 | MS.gene95676:intron | 20.0% |
| !!! | CGAATTGTTTCTAATTTTAT+CGG | + | chr7.4:33792954-33792973 | MS.gene95676:intron | 20.0% |
| !!! | TAATCTTTTTCAAAATGACA+TGG | + | chr7.4:33793038-33793057 | MS.gene95676:intron | 20.0% |
| ! | AACACATCAAAAAAGAAATG+CGG | - | chr7.4:33793319-33793338 | None:intergenic | 25.0% |
| ! | CCCACTTATAATATAATGAA+TGG | + | chr7.4:33793438-33793457 | MS.gene95676:intron | 25.0% |
| ! | CCCATTCATTATATTATAAG+TGG | - | chr7.4:33793442-33793461 | None:intergenic | 25.0% |
| ! | TGAAAAAGTTGTTGAAAATC+AGG | + | chr7.4:33793408-33793427 | MS.gene95676:CDS | 25.0% |
| !! | GATATTTGAAATTGCATTGT+AGG | + | chr7.4:33792871-33792890 | MS.gene95676:intron | 25.0% |
| !! | TAGTTATAACGCAATCATTT+TGG | + | chr7.4:33792631-33792650 | MS.gene95676:intron | 25.0% |
| AAAAACATGTCAAATTCAGC+TGG | - | chr7.4:33793125-33793144 | None:intergenic | 30.0% | |
| ACACATCAAAAAAGAAATGC+GGG | - | chr7.4:33793318-33793337 | None:intergenic | 30.0% | |
| ACTATGATACAAACAATTGG+AGG | - | chr7.4:33792615-33792634 | None:intergenic | 30.0% | |
| GACAATTGAAGATTTGATTG+AGG | + | chr7.4:33792546-33792565 | MS.gene95676:CDS | 30.0% | |
| TAGGGAATGTGAATTATAAG+TGG | + | chr7.4:33793180-33793199 | MS.gene95676:CDS | 30.0% | |
| TATAAGCAAGCATAACCTTA+AGG | + | chr7.4:33793256-33793275 | MS.gene95676:CDS | 30.0% | |
| AAGACGATACAAAAACACTC+AGG | - | chr7.4:33793490-33793509 | None:intergenic | 35.0% | |
| AGACGATACAAAAACACTCA+GGG | - | chr7.4:33793489-33793508 | None:intergenic | 35.0% | |
| GAGTGCAAACGAACAAAAAT+AGG | - | chr7.4:33793296-33793315 | None:intergenic | 35.0% | |
| GGAATGTGAATTATAAGTGG+AGG | + | chr7.4:33793183-33793202 | MS.gene95676:CDS | 35.0% | |
| GGTATAAGGATTATGCATCT+TGG | + | chr7.4:33793460-33793479 | MS.gene95676:intron | 35.0% | |
| TCAAGTGAATCTGATGTTCT+TGG | + | chr7.4:33793367-33793386 | MS.gene95676:CDS | 35.0% | |
| TCATTAGTGACTGTGGTTTA+TGG | + | chr7.4:33792745-33792764 | MS.gene95676:intron | 35.0% | |
| !!! | GAAATTTTTTGCAGGATTGC+AGG | + | chr7.4:33793079-33793098 | MS.gene95676:intron | 35.0% |
| ACAATTTGCGAGATAGAGAC+TGG | + | chr7.4:33792685-33792704 | MS.gene95676:CDS | 40.0% | |
| AGACAAACAGACGTTGTTCA+AGG | + | chr7.4:33793145-33793164 | MS.gene95676:CDS | 40.0% | |
| AGTCAAGAAAGCTACAACTG+AGG | + | chr7.4:33793214-33793233 | MS.gene95676:CDS | 40.0% | |
| AGTCTCTATCTCGCAAATTG+TGG | - | chr7.4:33792686-33792705 | None:intergenic | 40.0% | |
| AGTGAATCTGATGTTCTTGG+TGG | + | chr7.4:33793370-33793389 | MS.gene95676:CDS | 40.0% | |
| GACGATACAAAAACACTCAG+GGG | - | chr7.4:33793488-33793507 | None:intergenic | 40.0% | |
| TAGCACAACACTCTTGCATT+GGG | - | chr7.4:33792794-33792813 | None:intergenic | 40.0% | |
| TCGATCGAACAACACAAATG+TGG | - | chr7.4:33792937-33792956 | None:intergenic | 40.0% | |
| TTAGCACAACACTCTTGCAT+TGG | - | chr7.4:33792795-33792814 | None:intergenic | 40.0% | |
| ! | ACGTGTTTTCATATGCTGAC+AGG | + | chr7.4:33793342-33793361 | MS.gene95676:intron | 40.0% |
| ! | GTATCGTCTTCTGTGTTGTT+AGG | + | chr7.4:33793500-33793519 | MS.gene95676:intron | 40.0% |
| ! | TCTGTGTTGTTAGGAAGGAT+TGG | + | chr7.4:33793509-33793528 | MS.gene95676:intron | 40.0% |
| !! | AATGCAAGAGTGTTGTGCTA+AGG | + | chr7.4:33792794-33792813 | MS.gene95676:intron | 40.0% |
| !!! | ACGCAATCATTTTGGTTTGC+AGG | + | chr7.4:33792639-33792658 | MS.gene95676:intron | 40.0% |
| ATGCACGTCATTAGTGACTG+TGG | + | chr7.4:33792738-33792757 | MS.gene95676:CDS | 45.0% | |
| CAAACAGACGTTGTTCAAGG+TGG | + | chr7.4:33793148-33793167 | MS.gene95676:CDS | 45.0% | |
| GAACAAAAATAGGTGCGCGT+TGG | - | chr7.4:33793286-33793305 | None:intergenic | 45.0% | |
| GAAGATCTGCTCGAGGTATA+TGG | + | chr7.4:33792987-33793006 | MS.gene95676:intron | 45.0% | |
| GTTATCGTCATGAACGACAC+TGG | + | chr7.4:33792906-33792925 | MS.gene95676:intron | 45.0% | |
| TCAAGGTGGAGGTTAGCATA+GGG | + | chr7.4:33793162-33793181 | MS.gene95676:CDS | 45.0% | |
| TTCAAGGTGGAGGTTAGCAT+AGG | + | chr7.4:33793161-33793180 | MS.gene95676:CDS | 45.0% | |
| TTCTGTTGAAGATCTGCTCG+AGG | + | chr7.4:33792980-33792999 | MS.gene95676:intron | 45.0% | |
| ! | AGATAGAGACTGGTGTCGAA+TGG | + | chr7.4:33792695-33792714 | MS.gene95676:CDS | 45.0% |
| ! | CGTCTTCTGTGTTGTTAGGA+AGG | + | chr7.4:33793504-33793523 | MS.gene95676:intron | 45.0% |
| !! | TTTGGTTTGCAGGCATGTCT+TGG | + | chr7.4:33792649-33792668 | MS.gene95676:intron | 45.0% |
| ACAGACGTTGTTCAAGGTGG+AGG | + | chr7.4:33793151-33793170 | MS.gene95676:CDS | 50.0% | |
| GGATTGCAGGCACATAGTCA+AGG | + | chr7.4:33793092-33793111 | MS.gene95676:intron | 50.0% | |
| ! | GTGCGCGTTGGTAAACCTTA+AGG | - | chr7.4:33793274-33793293 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 33792544 | 33793551 | 33792544 | ID=MS.gene95676 |
| chr7.4 | mRNA | 33792544 | 33793551 | 33792544 | ID=MS.gene95676.t1;Parent=MS.gene95676 |
| chr7.4 | exon | 33792544 | 33792576 | 33792544 | ID=MS.gene95676.t1.exon1;Parent=MS.gene95676.t1 |
| chr7.4 | CDS | 33792544 | 33792576 | 33792544 | ID=cds.MS.gene95676.t1;Parent=MS.gene95676.t1 |
| chr7.4 | exon | 33792661 | 33792759 | 33792661 | ID=MS.gene95676.t1.exon2;Parent=MS.gene95676.t1 |
| chr7.4 | CDS | 33792661 | 33792759 | 33792661 | ID=cds.MS.gene95676.t1;Parent=MS.gene95676.t1 |
| chr7.4 | exon | 33793101 | 33793277 | 33793101 | ID=MS.gene95676.t1.exon3;Parent=MS.gene95676.t1 |
| chr7.4 | CDS | 33793101 | 33793277 | 33793101 | ID=cds.MS.gene95676.t1;Parent=MS.gene95676.t1 |
| chr7.4 | exon | 33793364 | 33793429 | 33793364 | ID=MS.gene95676.t1.exon4;Parent=MS.gene95676.t1 |
| chr7.4 | CDS | 33793364 | 33793429 | 33793364 | ID=cds.MS.gene95676.t1;Parent=MS.gene95676.t1 |
| chr7.4 | exon | 33793522 | 33793551 | 33793522 | ID=MS.gene95676.t1.exon5;Parent=MS.gene95676.t1 |
| chr7.4 | CDS | 33793522 | 33793551 | 33793522 | ID=cds.MS.gene95676.t1;Parent=MS.gene95676.t1 |
| Gene Sequence |
| Protein sequence |
