AlfalfaGEDB | MS.gene95738

M. sativa cultivar XinJiangDaYe / MS.gene95738

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95738.t1	AES87914.2	75.5	139	22	2	1	139	1	127	4.00E-44	187.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95738.t1	G7JSH2	75.5	139	22	2	1	139	1	127	2.9e-44	187.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050020	MS.gene95738	0.829536	4.87E-55	-1.69E-46
MS.gene050584	MS.gene95738	0.807483	4.94E-50	-1.69E-46
MS.gene050585	MS.gene95738	0.807436	5.06E-50	-1.69E-46
MS.gene050587	MS.gene95738	0.820896	5.38E-53	-1.69E-46
MS.gene05157	MS.gene95738	0.800958	1.13E-48	-1.69E-46
MS.gene051789	MS.gene95738	0.805097	1.57E-49	-1.69E-46
MS.gene052142	MS.gene95738	0.820624	6.22E-53	-1.69E-46
MS.gene053074	MS.gene95738	0.817385	3.39E-52	-1.69E-46
MS.gene053383	MS.gene95738	0.808935	2.43E-50	-1.69E-46
MS.gene05456	MS.gene95738	0.838904	2.18E-57	-1.69E-46
MS.gene055359	MS.gene95738	0.832903	7.25E-56	-1.69E-46
MS.gene059006	MS.gene95738	0.819728	9.97E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95738.t1	MTR_4g037480	83.453	139	11	2	1	139	1	127	6.46e-65	194
MS.gene95738.t1	MTR_4g037560	82.270	141	20	3	1	139	1	138	1.37e-59	181
MS.gene95738.t1	MTR_2g090185	52.518	139	53	5	1	139	1	126	6.70e-28	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95738.t1	AT2G20870	40.000	110	59	5	35	139	33	140	1.71e-12	60.8

Find 48 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCCTAATAATCCATTTAT+TGG	0.205431	4.1:-54781666	MS.gene95738:CDS
TACCCTGGCATTGGACATTT+AGG	0.231298	4.1:-54781711	MS.gene95738:CDS
CCTCCAGTGCCACCAATAAA+TGG	0.254443	4.1:+54781654	None:intergenic
GGAGGATCAGGTTCGGGTTT+AGG	0.265984	4.1:-54781636	MS.gene95738:CDS
CCACCACCACTGCCGGGATT+TGG	0.272217	4.1:+54781522	None:intergenic
CCACCAATAAATGGATTATT+AGG	0.286771	4.1:+54781663	None:intergenic
TAGGATTTCCACCGAAATTC+AGG	0.294433	4.1:-54781692	MS.gene95738:CDS
GGCACTGGAGGATCAGGTTC+GGG	0.362114	4.1:-54781642	MS.gene95738:CDS
TGTTCCAAACCCTGGCTTTG+AGG	0.364651	4.1:-54781547	MS.gene95738:CDS
GGAACCTCAAAGCCAGGGTT+TGG	0.367827	4.1:+54781543	None:intergenic
CCCGGCAGTGGTGGTGGCGG+TGG	0.380504	4.1:-54781516	MS.gene95738:CDS
TGGCACTGGAGGATCAGGTT+CGG	0.387534	4.1:-54781643	MS.gene95738:CDS
ATTGGTGGCACTGGAGGATC+AGG	0.397280	4.1:-54781648	MS.gene95738:CDS
GGCAGTGGTGGTGGCGGTGG+TGG	0.404146	4.1:-54781513	MS.gene95738:CDS
CTCTTTCTTGTCGTCGCTGT+CGG	0.412216	4.1:+54781773	None:intergenic
GGCTTTGAGGTTCCAAATCC+CGG	0.426165	4.1:-54781534	MS.gene95738:CDS
TCAGGTTCGGGTTTAGGATC+AGG	0.431514	4.1:-54781630	MS.gene95738:CDS
AATCCATTTATTGGTGGCAC+TGG	0.434316	4.1:-54781657	MS.gene95738:CDS
ACCACCGCCACCACCACTGC+CGG	0.441986	4.1:+54781515	None:intergenic
TCAGGTCATAGTTATGTTCC+CGG	0.442709	4.1:-54781585	MS.gene95738:CDS
TTAGGAGTAAGCCTGAATTT+CGG	0.443514	4.1:+54781681	None:intergenic
CCAAATCCCGGCAGTGGTGG+TGG	0.448808	4.1:-54781522	MS.gene95738:CDS
TCAGGAACAGGAGCAGGATC+AGG	0.471562	4.1:-54781612	MS.gene95738:CDS
GGAGCAGGATCAGGATCATC+AGG	0.472228	4.1:-54781603	MS.gene95738:CDS
GGATTTGGAACCTCAAAGCC+AGG	0.493115	4.1:+54781537	None:intergenic
CCTAATAATCCATTTATTGG+TGG	0.494125	4.1:-54781663	MS.gene95738:CDS
AACAAATGTGTCATCGCCAC+CGG	0.495697	4.1:+54781566	None:intergenic
ACAACACAGCAAGCAGTTGC+TGG	0.501170	4.1:-54781822	MS.gene95738:CDS
CCATTTATTGGTGGCACTGG+AGG	0.501560	4.1:-54781654	MS.gene95738:CDS
AATCCTAAATGTCCAATGCC+AGG	0.501871	4.1:+54781708	None:intergenic
TCGGGTTTAGGATCAGGAAC+AGG	0.510472	4.1:-54781624	MS.gene95738:CDS
GAGGTTCCAAATCCCGGCAG+TGG	0.510808	4.1:-54781528	MS.gene95738:CDS
CCACCGCCACCACCACTGCC+GGG	0.525610	4.1:+54781516	None:intergenic
AATCCCGGCAGTGGTGGTGG+CGG	0.528937	4.1:-54781519	MS.gene95738:CDS
AAACTGCATTACCCTGGCAT+TGG	0.536138	4.1:-54781720	MS.gene95738:CDS
TTAGGATCAGGAACAGGAGC+AGG	0.546096	4.1:-54781618	MS.gene95738:CDS
TGGTGGTAGAGTTGCATCAA+CGG	0.551049	4.1:-54781496	MS.gene95738:CDS
GACACATTTGTTCCAAACCC+TGG	0.566936	4.1:-54781555	MS.gene95738:CDS
CTGCTTGCTGTGTTGTGACA+AGG	0.573331	4.1:+54781829	None:intergenic
GTTCCAAATCCCGGCAGTGG+TGG	0.585291	4.1:-54781525	MS.gene95738:CDS
GGAGTAAGCCTGAATTTCGG+TGG	0.588643	4.1:+54781684	None:intergenic
ACAAATGTGTCATCGCCACC+GGG	0.594099	4.1:+54781567	None:intergenic
ATCCTAAATGTCCAATGCCA+GGG	0.617878	4.1:+54781709	None:intergenic
TCTTAGCATTAGCAATGCTG+CGG	0.631738	4.1:+54781799	None:intergenic
GATTTGGAACCTCAAAGCCA+GGG	0.635813	4.1:+54781538	None:intergenic
CATGGTAAACTGCATTACCC+TGG	0.658502	4.1:-54781726	MS.gene95738:CDS
CTTGCTGTGTTGTGACAAGG+AGG	0.720764	4.1:+54781832	None:intergenic
GGTCATAGTTATGTTCCCGG+TGG	0.737137	4.1:-54781582	MS.gene95738:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACTCCTAATAATCCATTTAT+TGG	-	chr4.1:54781701-54781720	MS.gene95738:CDS	25.0%
!!	TTTTTATTCAAGCATGATCA+TGG	-	chr4.1:54781623-54781642	MS.gene95738:CDS	25.0%
	CCTAATAATCCATTTATTGG+TGG	-	chr4.1:54781704-54781723	MS.gene95738:CDS	30.0%
	TCATGCTTGAATAAAAACTG+CGG	+	chr4.1:54781620-54781639	None:intergenic	30.0%
!!	CCACCAATAAATGGATTATT+AGG	+	chr4.1:54781707-54781726	None:intergenic	30.0%
	TTAGGAGTAAGCCTGAATTT+CGG	+	chr4.1:54781689-54781708	None:intergenic	35.0%
	AATCCTAAATGTCCAATGCC+AGG	+	chr4.1:54781662-54781681	None:intergenic	40.0%
	ATCCTAAATGTCCAATGCCA+GGG	+	chr4.1:54781661-54781680	None:intergenic	40.0%
	TAGGATTTCCACCGAAATTC+AGG	-	chr4.1:54781675-54781694	MS.gene95738:CDS	40.0%
	TCAGGTCATAGTTATGTTCC+CGG	-	chr4.1:54781782-54781801	MS.gene95738:CDS	40.0%
	TCTTAGCATTAGCAATGCTG+CGG	+	chr4.1:54781571-54781590	None:intergenic	40.0%
!	AATCCATTTATTGGTGGCAC+TGG	-	chr4.1:54781710-54781729	MS.gene95738:CDS	40.0%
	AAACTGCATTACCCTGGCAT+TGG	-	chr4.1:54781647-54781666	MS.gene95738:CDS	45.0%
	AACAAATGTGTCATCGCCAC+CGG	+	chr4.1:54781804-54781823	None:intergenic	45.0%
	CATGGTAAACTGCATTACCC+TGG	-	chr4.1:54781641-54781660	MS.gene95738:CDS	45.0%
	GACACATTTGTTCCAAACCC+TGG	-	chr4.1:54781812-54781831	MS.gene95738:CDS	45.0%
	TGGTGGTAGAGTTGCATCAA+CGG	-	chr4.1:54781871-54781890	MS.gene95738:CDS	45.0%
!	GATTTGGAACCTCAAAGCCA+GGG	+	chr4.1:54781832-54781851	None:intergenic	45.0%
!	TACCCTGGCATTGGACATTT+AGG	-	chr4.1:54781656-54781675	MS.gene95738:CDS	45.0%
	ACAAATGTGTCATCGCCACC+GGG	+	chr4.1:54781803-54781822	None:intergenic	50.0%
	ACAACACAGCAAGCAGTTGC+TGG	-	chr4.1:54781545-54781564	MS.gene95738:CDS	50.0%
	CCTCCAGTGCCACCAATAAA+TGG	+	chr4.1:54781716-54781735	None:intergenic	50.0%
	CTCTTTCTTGTCGTCGCTGT+CGG	+	chr4.1:54781597-54781616	None:intergenic	50.0%
	GGAGTAAGCCTGAATTTCGG+TGG	+	chr4.1:54781686-54781705	None:intergenic	50.0%
	GGTCATAGTTATGTTCCCGG+TGG	-	chr4.1:54781785-54781804	MS.gene95738:CDS	50.0%
	TTAGGATCAGGAACAGGAGC+AGG	-	chr4.1:54781749-54781768	MS.gene95738:CDS	50.0%
!	CCATTTATTGGTGGCACTGG+AGG	-	chr4.1:54781713-54781732	MS.gene95738:CDS	50.0%
!	CTTGCTGTGTTGTGACAAGG+AGG	+	chr4.1:54781538-54781557	None:intergenic	50.0%
!	GGATTTGGAACCTCAAAGCC+AGG	+	chr4.1:54781833-54781852	None:intergenic	50.0%
!	TCAGGTTCGGGTTTAGGATC+AGG	-	chr4.1:54781737-54781756	MS.gene95738:CDS	50.0%
!	TCGGGTTTAGGATCAGGAAC+AGG	-	chr4.1:54781743-54781762	MS.gene95738:CDS	50.0%
!!	CTGCTTGCTGTGTTGTGACA+AGG	+	chr4.1:54781541-54781560	None:intergenic	50.0%
!!	GGCTTTGAGGTTCCAAATCC+CGG	-	chr4.1:54781833-54781852	MS.gene95738:CDS	50.0%
!!	TGTTCCAAACCCTGGCTTTG+AGG	-	chr4.1:54781820-54781839	MS.gene95738:CDS	50.0%
	GGAACCTCAAAGCCAGGGTT+TGG	+	chr4.1:54781827-54781846	None:intergenic	55.0%
	GGAGCAGGATCAGGATCATC+AGG	-	chr4.1:54781764-54781783	MS.gene95738:CDS	55.0%
	TCAGGAACAGGAGCAGGATC+AGG	-	chr4.1:54781755-54781774	MS.gene95738:CDS	55.0%
!	ATTGGTGGCACTGGAGGATC+AGG	-	chr4.1:54781719-54781738	MS.gene95738:CDS	55.0%
!	GGAGGATCAGGTTCGGGTTT+AGG	-	chr4.1:54781731-54781750	MS.gene95738:CDS	55.0%
!	TGGCACTGGAGGATCAGGTT+CGG	-	chr4.1:54781724-54781743	MS.gene95738:CDS	55.0%
	GAGGTTCCAAATCCCGGCAG+TGG	-	chr4.1:54781839-54781858	MS.gene95738:CDS	60.0%
	GTTCCAAATCCCGGCAGTGG+TGG	-	chr4.1:54781842-54781861	MS.gene95738:CDS	60.0%
!	GGCACTGGAGGATCAGGTTC+GGG	-	chr4.1:54781725-54781744	MS.gene95738:CDS	60.0%
	CCAAATCCCGGCAGTGGTGG+TGG	-	chr4.1:54781845-54781864	MS.gene95738:CDS	65.0%
	CCACCACCACTGCCGGGATT+TGG	+	chr4.1:54781848-54781867	None:intergenic	65.0%
!!	AATCCCGGCAGTGGTGGTGG+CGG	-	chr4.1:54781848-54781867	MS.gene95738:CDS	65.0%
	ACCACCGCCACCACCACTGC+CGG	+	chr4.1:54781855-54781874	None:intergenic	70.0%
	CCACCGCCACCACCACTGCC+GGG	+	chr4.1:54781854-54781873	None:intergenic	75.0%
!!	GGCAGTGGTGGTGGCGGTGG+TGG	-	chr4.1:54781854-54781873	MS.gene95738:CDS	75.0%
!!!	CCCGGCAGTGGTGGTGGCGG+TGG	-	chr4.1:54781851-54781870	MS.gene95738:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	54781485	54781904	54781485	ID=MS.gene95738
chr4.1	mRNA	54781485	54781904	54781485	ID=MS.gene95738.t1;Parent=MS.gene95738
chr4.1	exon	54781485	54781904	54781485	ID=MS.gene95738.t1.exon1;Parent=MS.gene95738.t1
chr4.1	CDS	54781485	54781904	54781485	ID=cds.MS.gene95738.t1;Parent=MS.gene95738.t1

Gene Sequence

>MS.gene95738

ATGGTTCAAAGAGCTTACTCTTTCTTTGCACTTTTTCTCATTTTCAATATCCTCCTTGTCACAACACAGCAAGCAGTTGCTGGCCGCAGCATTGCTAATGCTAAGAACTCCGACAGCGACGACAAGAAAGAGCCGCAGTTTTTATTCAAGCATGATCATGGTAAACTGCATTACCCTGGCATTGGACATTTAGGATTTCCACCGAAATTCAGGCTTACTCCTAATAATCCATTTATTGGTGGCACTGGAGGATCAGGTTCGGGTTTAGGATCAGGAACAGGAGCAGGATCAGGATCATCAGGTCATAGTTATGTTCCCGGTGGCGATGACACATTTGTTCCAAACCCTGGCTTTGAGGTTCCAAATCCCGGCAGTGGTGGTGGCGGTGGTGGTAGAGTTGCATCAACGGTTCATCCATGA

Protein sequence

>MS.gene95738.t1

MVQRAYSFFALFLIFNILLVTTQQAVAGRSIANAKNSDSDDKKEPQFLFKHDHGKLHYPGIGHLGFPPKFRLTPNNPFIGGTGGSGSGLGSGTGAGSGSSGHSYVPGGDDTFVPNPGFEVPNPGSGGGGGGRVASTVHP