AlfalfaGEDB | MS.gene95761

M. sativa cultivar XinJiangDaYe / MS.gene95761

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005575 GO:0008194 GO:0008375 GO:0016020 GO:0016740 GO:0016757 GO:0016758 GO:0110165
PFAM: PF02485.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95761.t1	XP_013442870.1	97.9	144	3	0	1	144	290	433	1.80E-79	305.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95761.t1	Q9FLD7	61.4	145	54	2	1	143	302	446	1.6e-49	196.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95761.t1	G7ZX28	97.9	144	3	0	1	144	290	433	1.3e-79	305.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene95761	0.814115	1.82E-51	-1.69E-46
MS.gene049499	MS.gene95761	0.80768	4.49E-50	-1.69E-46
MS.gene049983	MS.gene95761	0.801356	9.36E-49	-1.69E-46
MS.gene052037	MS.gene95761	0.801214	1.00E-48	-1.69E-46
MS.gene052463	MS.gene95761	0.833017	6.79E-56	-1.69E-46
MS.gene052773	MS.gene95761	0.803145	4.01E-49	-1.69E-46
MS.gene054651	MS.gene95761	0.81192	5.50E-51	-1.69E-46
MS.gene057102	MS.gene95761	0.802853	4.61E-49	-1.69E-46
MS.gene058897	MS.gene95761	0.814648	1.38E-51	-1.69E-46
MS.gene060932	MS.gene95761	0.811802	5.83E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95761.t1	MTR_0072s0020	97.917	144	3	0	1	144	290	433	1.49e-104	305
MS.gene95761.t1	MTR_6g011080	59.589	146	57	2	1	144	288	433	1.44e-59	190
MS.gene95761.t1	MTR_4g022240	54.483	145	64	2	1	143	274	418	1.00e-52	172
MS.gene95761.t1	MTR_7g086720	55.102	147	60	5	1	143	254	398	6.44e-48	159
MS.gene95761.t1	MTR_6g027550	52.703	148	65	4	1	143	169	316	2.24e-47	155
MS.gene95761.t1	MTR_0662s0030	54.783	115	51	1	1	114	177	291	9.52e-42	140
MS.gene95761.t1	MTR_1g108280	45.890	146	76	2	1	143	251	396	2.33e-41	142
MS.gene95761.t1	MTR_2g085300	50.746	134	60	2	1	133	284	412	3.21e-41	142
MS.gene95761.t1	MTR_4g114140	54.783	115	51	1	1	114	276	390	7.52e-41	140
MS.gene95761.t1	MTR_1g046740	41.781	146	80	4	1	144	297	439	1.57e-30	114
MS.gene95761.t1	MTR_4g008010	36.301	146	89	4	1	143	262	406	2.20e-22	91.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95761.t1	AT4G03340	79.021	143	30	0	1	143	282	424	9.55e-85	254
MS.gene95761.t1	AT4G03340	79.021	143	30	0	1	143	305	447	1.16e-84	255
MS.gene95761.t1	AT1G03520	72.727	143	39	0	1	143	215	357	7.79e-79	237
MS.gene95761.t1	AT1G03520	72.727	143	39	0	1	143	304	446	6.19e-78	238
MS.gene95761.t1	AT5G39990	61.379	145	54	2	1	143	302	446	1.25e-61	196
MS.gene95761.t1	AT5G15050	57.931	145	59	2	1	143	289	433	1.15e-59	190
MS.gene95761.t1	AT4G27480	51.034	145	64	3	1	143	281	420	3.80e-48	160
MS.gene95761.t1	AT4G27480	51.034	145	64	3	1	143	281	420	3.80e-48	160
MS.gene95761.t1	AT4G27480	51.034	145	64	3	1	143	281	420	3.80e-48	160
MS.gene95761.t1	AT1G53100	52.381	147	62	3	1	144	282	423	1.33e-47	159
MS.gene95761.t1	AT1G53100	55.224	134	53	2	1	132	282	410	2.92e-46	155
MS.gene95761.t1	AT1G71070	48.966	145	72	2	1	143	250	394	2.86e-43	147
MS.gene95761.t1	AT3G15350	52.518	139	58	2	1	136	282	415	6.80e-43	146
MS.gene95761.t1	AT3G15350	52.518	139	58	2	1	136	282	415	6.80e-43	146
MS.gene95761.t1	AT3G03690	60.440	91	36	0	1	91	254	344	1.84e-37	131
MS.gene95761.t1	AT2G37585	45.833	144	59	5	1	143	258	383	2.34e-33	120
MS.gene95761.t1	AT3G24040	42.466	146	82	2	1	144	270	415	8.86e-32	117

Find 28 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGATTGACAAAGAACTTT+TGG	0.219774	4.1:-53814883	MS.gene95761:CDS
CGCCAGGGGCATGGTGTATT+GGG	0.263740	4.1:-53814842	MS.gene95761:CDS
TCGCCAGGGGCATGGTGTAT+TGG	0.295627	4.1:-53814843	MS.gene95761:CDS
TATGAAGAACTTCGACAAAA+TGG	0.301478	4.1:-53814961	MS.gene95761:CDS
CAAGCTACATGAGCTGCTTC+AGG	0.370203	4.1:-53814745	MS.gene95761:CDS
TCTAGGACTGGATCATCCTT+TGG	0.394479	4.1:+53814906	None:intergenic
ACGATCTTCATTACATTGCT+TGG	0.406358	4.1:-53815019	MS.gene95761:CDS
TTGTCAATCTTGTCTAGGAC+TGG	0.411892	4.1:+53814894	None:intergenic
CGATCTTCATTACATTGCTT+GGG	0.425542	4.1:-53815018	MS.gene95761:CDS
AGAGACATTGGATGTTGCTT+TGG	0.445772	4.1:+53814987	None:intergenic
GACATTGGATGTTGCTTTGG+TGG	0.449058	4.1:+53814990	None:intergenic
GTTCTTTGTCAATCTTGTCT+AGG	0.464391	4.1:+53814889	None:intergenic
AGCTTGTCAGCACCAGGTCC+TGG	0.464926	4.1:+53814762	None:intergenic
GCGGCAATGATACAGTGTTT+AGG	0.469086	4.1:-53814785	MS.gene95761:CDS
GGCAAAGGGAGCATTGCTCT+TGG	0.470894	4.1:+53814938	None:intergenic
TTCTTCATAGTTAGAGACAT+TGG	0.471675	4.1:+53814975	None:intergenic
TGGTGTATTGGGTCATCAGA+TGG	0.511556	4.1:-53814831	MS.gene95761:CDS
TCATTGCCGCGCAAAGAGCA+TGG	0.515746	4.1:+53814798	None:intergenic
TCATGTAGCTTGTCAGCACC+AGG	0.546463	4.1:+53814756	None:intergenic
GACCCAATACACCATGCCCC+TGG	0.553237	4.1:+53814840	None:intergenic
CTTTGCCCGAAAATTTCCAA+AGG	0.565068	4.1:-53814922	MS.gene95761:CDS
AATGATACAGTGTTTAGGCC+AGG	0.579640	4.1:-53814780	MS.gene95761:CDS
ACAGTGTGAAAATATCCCTC+TGG	0.594600	4.1:+53815083	None:intergenic
CATTGCCGCGCAAAGAGCAT+GGG	0.598442	4.1:+53814799	None:intergenic
GCAGACCCATGCTCTTTGCG+CGG	0.603282	4.1:-53814804	MS.gene95761:CDS
ACAGTGTTTAGGCCAGGACC+TGG	0.608879	4.1:-53814774	MS.gene95761:CDS
AAGCAATGTAATGAAGATCG+TGG	0.688506	4.1:+53815021	None:intergenic
TGTATTGGGTCATCAGATGG+TGG	0.706381	4.1:-53814828	MS.gene95761:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTTGCAACACTCAAAAATTT+CGG	-	chr4.1:53814762-53814781	MS.gene95761:CDS	25.0%
!!	AAGATTGACAAAGAACTTTT+GGG	-	chr4.1:53814938-53814957	MS.gene95761:CDS	25.0%
	TATGAAGAACTTCGACAAAA+TGG	-	chr4.1:53814859-53814878	MS.gene95761:CDS	30.0%
	TTCTTCATAGTTAGAGACAT+TGG	+	chr4.1:53814848-53814867	None:intergenic	30.0%
!	AGATTGACAAAGAACTTTTG+GGG	-	chr4.1:53814939-53814958	MS.gene95761:CDS	30.0%
!	CAAGATTGACAAAGAACTTT+TGG	-	chr4.1:53814937-53814956	MS.gene95761:CDS	30.0%
!!	ATCATCCTTTGGAAATTTTC+GGG	+	chr4.1:53814906-53814925	None:intergenic	30.0%
!!	GATCATCCTTTGGAAATTTT+CGG	+	chr4.1:53814907-53814926	None:intergenic	30.0%
	AAGCAATGTAATGAAGATCG+TGG	+	chr4.1:53814802-53814821	None:intergenic	35.0%
	ACGATCTTCATTACATTGCT+TGG	-	chr4.1:53814801-53814820	MS.gene95761:CDS	35.0%
	CACTCAAAAATTTCGGCATA+CGG	-	chr4.1:53814769-53814788	MS.gene95761:CDS	35.0%
	CGATCTTCATTACATTGCTT+GGG	-	chr4.1:53814802-53814821	MS.gene95761:CDS	35.0%
	GTTCTTTGTCAATCTTGTCT+AGG	+	chr4.1:53814934-53814953	None:intergenic	35.0%
!!	TTTGGAAATTTTCGGGCAAA+GGG	+	chr4.1:53814899-53814918	None:intergenic	35.0%
	AATGATACAGTGTTTAGGCC+AGG	-	chr4.1:53815040-53815059	MS.gene95761:CDS	40.0%
	ACAGTGTGAAAATATCCCTC+TGG	+	chr4.1:53814740-53814759	None:intergenic	40.0%
	CTTTGCCCGAAAATTTCCAA+AGG	-	chr4.1:53814898-53814917	MS.gene95761:CDS	40.0%
!	AAATTTTGTCTCTTCGCCAG+AGG	-	chr4.1:53814721-53814740	MS.gene95761:CDS	40.0%
!	AATTTTGTCTCTTCGCCAGA+GGG	-	chr4.1:53814722-53814741	MS.gene95761:CDS	40.0%
!	AGAGACATTGGATGTTGCTT+TGG	+	chr4.1:53814836-53814855	None:intergenic	40.0%
!	TTGTCAATCTTGTCTAGGAC+TGG	+	chr4.1:53814929-53814948	None:intergenic	40.0%
!!	CTTTGGAAATTTTCGGGCAA+AGG	+	chr4.1:53814900-53814919	None:intergenic	40.0%
	GCGGCAATGATACAGTGTTT+AGG	-	chr4.1:53815035-53815054	MS.gene95761:CDS	45.0%
	TGGTGTATTGGGTCATCAGA+TGG	-	chr4.1:53814989-53815008	MS.gene95761:CDS	45.0%
	TGTATTGGGTCATCAGATGG+TGG	-	chr4.1:53814992-53815011	MS.gene95761:CDS	45.0%
!	TCTAGGACTGGATCATCCTT+TGG	+	chr4.1:53814917-53814936	None:intergenic	45.0%
!!	GACATTGGATGTTGCTTTGG+TGG	+	chr4.1:53814833-53814852	None:intergenic	45.0%
	CAAGCTACATGAGCTGCTTC+AGG	-	chr4.1:53815075-53815094	MS.gene95761:CDS	50.0%
!	CACACATAGGTTTTCGCCAG+GGG	-	chr4.1:53814964-53814983	MS.gene95761:CDS	50.0%
!	GCACACATAGGTTTTCGCCA+GGG	-	chr4.1:53814963-53814982	MS.gene95761:CDS	50.0%
!	TCATGTAGCTTGTCAGCACC+AGG	+	chr4.1:53815067-53815086	None:intergenic	50.0%
!!!	AACTTTTGGGGCGCACACAT+AGG	-	chr4.1:53814951-53814970	MS.gene95761:CDS	50.0%
	ACAGTGTTTAGGCCAGGACC+TGG	-	chr4.1:53815046-53815065	MS.gene95761:CDS	55.0%
	TCATTGCCGCGCAAAGAGCA+TGG	+	chr4.1:53815025-53815044	None:intergenic	55.0%
!	ATAGGTTTTCGCCAGGGGCA+TGG	-	chr4.1:53814969-53814988	MS.gene95761:CDS	55.0%
!	CATTGCCGCGCAAAGAGCAT+GGG	+	chr4.1:53815024-53815043	None:intergenic	55.0%
!	CGCACACATAGGTTTTCGCC+AGG	-	chr4.1:53814962-53814981	MS.gene95761:CDS	55.0%
!!	GGCAAAGGGAGCATTGCTCT+TGG	+	chr4.1:53814885-53814904	None:intergenic	55.0%
	AGCTTGTCAGCACCAGGTCC+TGG	+	chr4.1:53815061-53815080	None:intergenic	60.0%
	GACCCAATACACCATGCCCC+TGG	+	chr4.1:53814983-53815002	None:intergenic	60.0%
	GCAGACCCATGCTCTTTGCG+CGG	-	chr4.1:53815016-53815035	MS.gene95761:CDS	60.0%
!	CGCCAGGGGCATGGTGTATT+GGG	-	chr4.1:53814978-53814997	MS.gene95761:CDS	60.0%
!	TCGCCAGGGGCATGGTGTAT+TGG	-	chr4.1:53814977-53814996	MS.gene95761:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	53814704	53815138	53814704	ID=MS.gene95761
chr4.1	mRNA	53814704	53815138	53814704	ID=MS.gene95761.t1;Parent=MS.gene95761
chr4.1	exon	53814704	53815138	53814704	ID=MS.gene95761.t1.exon1;Parent=MS.gene95761.t1
chr4.1	CDS	53814704	53815138	53814704	ID=cds.MS.gene95761.t1;Parent=MS.gene95761.t1

Gene Sequence

>MS.gene95761

ATGCTTATGTATTATACAAATTTTGTCTCTTCGCCAGAGGGATATTTTCACACTGTCATTTGCAACACTCAAAAATTTCGGCATACGGCAATAAGCCACGATCTTCATTACATTGCTTGGGACAGTCCACCAAAGCAACATCCAATGTCTCTAACTATGAAGAACTTCGACAAAATGGCCAAGAGCAATGCTCCCTTTGCCCGAAAATTTCCAAAGGATGATCCAGTCCTAGACAAGATTGACAAAGAACTTTTGGGGCGCACACATAGGTTTTCGCCAGGGGCATGGTGTATTGGGTCATCAGATGGTGGAGCAGACCCATGCTCTTTGCGCGGCAATGATACAGTGTTTAGGCCAGGACCTGGTGCTGACAAGCTACATGAGCTGCTTCAGGCGCTGTTATCTGATGAATTTCGAAGCAAACAGTGTTTGTGA

Protein sequence

>MS.gene95761.t1

MLMYYTNFVSSPEGYFHTVICNTQKFRHTAISHDLHYIAWDSPPKQHPMSLTMKNFDKMAKSNAPFARKFPKDDPVLDKIDKELLGRTHRFSPGAWCIGSSDGGADPCSLRGNDTVFRPGPGADKLHELLQALLSDEFRSKQCL