AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95877


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95877.t1 MTR_8g106580 78.716 296 9 2 1 242 1 296 3.48e-158 440
MS.gene95877.t1 MTR_6g081930 76.687 163 38 0 80 242 132 294 1.67e-86 259
MS.gene95877.t1 MTR_4g075730 39.623 318 109 10 1 241 6 317 2.74e-61 195
MS.gene95877.t1 MTR_0154s0010 57.037 135 58 0 108 242 1 135 9.66e-55 172
MS.gene95877.t1 MTR_2g101870 37.621 311 122 10 1 242 2 309 9.10e-52 170
MS.gene95877.t1 MTR_6g087880 41.463 164 92 3 80 242 146 306 1.46e-37 134
MS.gene95877.t1 MTR_8g086610 31.321 265 132 8 20 241 21 278 3.21e-32 119
MS.gene95877.t1 MTR_3g037650 36.310 168 100 3 77 242 111 273 5.30e-29 110
MS.gene95877.t1 MTR_4g075730 38.288 222 56 9 1 147 6 221 3.56e-28 107
MS.gene95877.t1 MTR_4g094800 49.123 114 37 6 1 93 12 125 4.29e-17 76.6
MS.gene95877.t1 MTR_3g033530 31.973 147 83 5 88 224 190 329 7.03e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95877.t1 AT5G42860 49.051 316 85 6 1 240 1 316 7.55e-81 245
MS.gene95877.t1 AT1G45688 44.083 338 91 7 1 240 1 338 9.57e-73 225
MS.gene95877.t1 AT4G35170 42.857 161 91 1 81 240 137 297 4.82e-45 153
MS.gene95877.t1 AT1G45688 42.857 238 55 7 1 157 1 238 1.81e-42 145
MS.gene95877.t1 AT2G41990 36.513 304 119 10 1 239 1 295 6.29e-41 142
MS.gene95877.t1 AT3G24600 36.875 160 98 2 81 239 144 301 1.71e-28 110
MS.gene95877.t1 AT3G24605 41.935 124 69 2 98 220 156 277 2.38e-24 99.0

Find 54 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTGTTGTTGTTGAGTTTC+CGG 0.246762 4.3:-794820 None:intergenic
AGCTTATGTGCTTGGGAAAT+TGG 0.250083 4.3:+795796 MS.gene95877:CDS
ATCAATTTCCTTCCATGGTT+TGG 0.270924 4.3:-794855 None:intergenic
TCCTATTCAGAAATCGTAAT+TGG 0.272853 4.3:+795290 MS.gene95877:CDS
GCCAATTACGATTTCTGAAT+AGG 0.334846 4.3:-795291 None:intergenic
CCATTCCACTTCTCAATCAT+AGG 0.363070 4.3:-795909 None:intergenic
CAAACCAAAATACTACAAAA+AGG 0.374910 4.3:+795820 MS.gene95877:CDS
GTTGTTGTTGTTGAGTTTCC+GGG 0.376955 4.3:-794819 None:intergenic
TTTAATGGCACTGGTAAAGT+TGG 0.388529 4.3:-795749 None:intergenic
GAACCCATTGGACTGATAAC+AGG 0.421746 4.3:-794755 None:intergenic
AGATCTAGAGCTTATGTGCT+TGG 0.422698 4.3:+795788 MS.gene95877:CDS
GATCTAGAGCTTATGTGCTT+GGG 0.431536 4.3:+795789 MS.gene95877:CDS
TCTTCCTCTAGCCGTTTCTC+TGG 0.434741 4.3:+794794 MS.gene95877:CDS
CTAGCCGTTTCTCTGGTTCC+CGG 0.451437 4.3:+794801 MS.gene95877:CDS
ATTACATCAATTTCCTTCCA+TGG 0.454352 4.3:-794860 None:intergenic
AGTAGAATGAAAGGAGGTAG+TGG 0.462601 4.3:-794732 None:intergenic
GCTAGTCTTAGCAGCACAAC+TGG 0.471971 4.3:+795722 MS.gene95877:CDS
TACTCCTGTTATCAGTCCAA+TGG 0.474596 4.3:+794751 MS.gene95877:CDS
ACTCCTGTTATCAGTCCAAT+GGG 0.485989 4.3:+794752 MS.gene95877:CDS
ATTAGTGAGTGTGGCAGTGA+TGG 0.497703 4.3:+795670 MS.gene95877:CDS
TAGCACCACTTCCATACAAT+GGG 0.499784 4.3:-795703 None:intergenic
TCCATGGTTTGGTGTTACTA+CGG 0.502227 4.3:-794844 None:intergenic
GGAAGCAAGATCCCATTGTA+TGG 0.504734 4.3:+795692 MS.gene95877:CDS
TCTGAATAGGATAGATCTAA+AGG 0.513188 4.3:-795278 None:intergenic
CAAGCTGGTTCAGATGCAAC+GGG 0.522817 4.3:+795164 MS.gene95877:CDS
TGGTTCAGATGCAACGGGTG+TGG 0.528116 4.3:+795169 MS.gene95877:CDS
AAGATCCCATTGTATGGAAG+TGG 0.539938 4.3:+795698 MS.gene95877:CDS
TCAGAAATCGTAATTGGCAC+TGG 0.540742 4.3:+795296 MS.gene95877:CDS
TTAGTGAGTGTGGCAGTGAT+GGG 0.543651 4.3:+795671 MS.gene95877:CDS
CATAGGTGCAAGACTTCTTG+AGG 0.543718 4.3:-795892 None:intergenic
ATAGGTGCAAGACTTCTTGA+GGG 0.555809 4.3:-795891 None:intergenic
CTAGAGGAAGAGTGAGAATG+AGG 0.556981 4.3:-794782 None:intergenic
GATAACAGGAGTAGAATGAA+AGG 0.557627 4.3:-794741 None:intergenic
TCAAGCTGGTTCAGATGCAA+CGG 0.562028 4.3:+795163 MS.gene95877:CDS
GGACTTTGAACATAGTATGC+TGG 0.578237 4.3:-794689 None:intergenic
GAGCATGTTAGAGTTCAAGC+TGG 0.583891 4.3:+795149 MS.gene95877:CDS
TAGTAACACCAAACCATGGA+AGG 0.584156 4.3:+794847 MS.gene95877:CDS
AACAGGAGTAGAATGAAAGG+AGG 0.584271 4.3:-794738 None:intergenic
GAGGAAGAGTGAGAATGAGG+AGG 0.592438 4.3:-794779 None:intergenic
GTTCAAAGTCCATCCCATGA+TGG 0.593197 4.3:+794701 MS.gene95877:CDS
GAAATTCACTTATCGTAACA+AGG 0.596007 4.3:+795223 MS.gene95877:CDS
GAATGAGGAGGAGAACCCAT+TGG 0.598887 4.3:-794767 None:intergenic
GTTTCCGGGAACCAGAGAAA+CGG 0.599995 4.3:-794805 None:intergenic
CTAGCACCACTTCCATACAA+TGG 0.615096 4.3:-795704 None:intergenic
GAGTCAAAGATTAGTGAGTG+TGG 0.625033 4.3:+795661 MS.gene95877:CDS
ACATAGTATGCTGGTCGACG+AGG 0.635101 4.3:-794680 None:intergenic
ATTCACTGTAATCATATCAG+TGG 0.649253 4.3:-795192 None:intergenic
ATAGGATAGATCTAAAGGTG+TGG 0.651020 4.3:-795273 None:intergenic
GGAACCAGAGAAACGGCTAG+AGG 0.666320 4.3:-794798 None:intergenic
AAATTCACTTATCGTAACAA+GGG 0.671507 4.3:+795224 MS.gene95877:CDS
ATGGTTTGGTGTTACTACGG+TGG 0.675852 4.3:-794841 None:intergenic
ACCGTAGTAACACCAAACCA+TGG 0.681305 4.3:+794843 MS.gene95877:CDS
TCGACGAGGAGATCTTGTTG+TGG 0.700688 4.3:-794666 None:intergenic
GCATACAAAGACAGACTCAG+AGG 0.705617 4.3:+794622 MS.gene95877:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATTGTTATGTCAAAT+GGG + chr4.3:795078-795097 MS.gene95877:intron 15.0%
!!! CTTTTAATTGTTATGTCAAA+TGG + chr4.3:795077-795096 MS.gene95877:intron 20.0%
! AAATTCACTTATCGTAACAA+GGG + chr4.3:795224-795243 MS.gene95877:CDS 25.0%
! ATATGATTATCGTGTAAACT+CGG - chr4.3:795436-795455 None:intergenic 25.0%
! ATTGATGTAATTGAAGAAGA+AGG + chr4.3:794872-794891 MS.gene95877:intron 25.0%
! CAAACCAAAATACTACAAAA+AGG + chr4.3:795820-795839 MS.gene95877:CDS 25.0%
!! ATCTCAAAGCTCAATTTTAA+TGG - chr4.3:795767-795786 None:intergenic 25.0%
!! TGAACCTTTTTGTAGTATTT+TGG - chr4.3:795827-795846 None:intergenic 25.0%
!! TGTGTATCAATCTTTTTCTT+TGG + chr4.3:795598-795617 MS.gene95877:intron 25.0%
!!! AACAGAAACATTGATTTTCT+TGG - chr4.3:795870-795889 None:intergenic 25.0%
!!! ACAGAAACATTGATTTTCTT+GGG - chr4.3:795869-795888 None:intergenic 25.0%
!!! TGAAGAAGTTTTATCAATCA+AGG + chr4.3:795636-795655 MS.gene95877:CDS 25.0%
AGTATACCTTAATCATGACA+TGG - chr4.3:795030-795049 None:intergenic 30.0%
ATTACATCAATTTCCTTCCA+TGG - chr4.3:794863-794882 None:intergenic 30.0%
ATTCACTGTAATCATATCAG+TGG - chr4.3:795195-795214 None:intergenic 30.0%
GAAATTCACTTATCGTAACA+AGG + chr4.3:795223-795242 MS.gene95877:CDS 30.0%
GAATTCAGATTGATTGCAAT+CGG + chr4.3:795404-795423 MS.gene95877:intron 30.0%
GTATACCTTAATCATGACAT+GGG - chr4.3:795029-795048 None:intergenic 30.0%
TATACCTTAATCATGACATG+GGG - chr4.3:795028-795047 None:intergenic 30.0%
TATGATTATCGTGTAAACTC+GGG - chr4.3:795435-795454 None:intergenic 30.0%
TATTTCCTTGCTTTCATTCT+TGG + chr4.3:794941-794960 MS.gene95877:intron 30.0%
TCCTATTCAGAAATCGTAAT+TGG + chr4.3:795290-795309 MS.gene95877:CDS 30.0%
TCTGAATAGGATAGATCTAA+AGG - chr4.3:795281-795300 None:intergenic 30.0%
! TCTTTTCTCTCTTATCCTTT+GGG + chr4.3:794982-795001 MS.gene95877:intron 30.0%
!! AGAAGTTTTATCAATCAAGG+AGG + chr4.3:795639-795658 MS.gene95877:CDS 30.0%
AAAAGCCAAGAATGAAAGCA+AGG - chr4.3:794949-794968 None:intergenic 35.0%
ACAACAATCGATACACTGAT+TGG + chr4.3:795510-795529 MS.gene95877:intron 35.0%
ATAGGATAGATCTAAAGGTG+TGG - chr4.3:795276-795295 None:intergenic 35.0%
ATCAATTTCCTTCCATGGTT+TGG - chr4.3:794858-794877 None:intergenic 35.0%
GAGAGAAAAGAGTGAACAAA+AGG - chr4.3:794973-794992 None:intergenic 35.0%
GATAACAGGAGTAGAATGAA+AGG - chr4.3:794744-794763 None:intergenic 35.0%
GCCAATTACGATTTCTGAAT+AGG - chr4.3:795294-795313 None:intergenic 35.0%
! CTCTTTTCTCTCTTATCCTT+TGG + chr4.3:794981-795000 MS.gene95877:intron 35.0%
! CTTTTCTCTCTTATCCTTTG+GGG + chr4.3:794983-795002 MS.gene95877:intron 35.0%
!! AAGCTCAATTTTAATGGCAC+TGG - chr4.3:795761-795780 None:intergenic 35.0%
!! TTTAATGGCACTGGTAAAGT+TGG - chr4.3:795752-795771 None:intergenic 35.0%
!!! AGTGGTTTTTTCACCATCAT+GGG - chr4.3:794717-794736 None:intergenic 35.0%
!!! TAGTGGTTTTTTCACCATCA+TGG - chr4.3:794718-794737 None:intergenic 35.0%
AACAGGAGTAGAATGAAAGG+AGG - chr4.3:794741-794760 None:intergenic 40.0%
AAGATCCCATTGTATGGAAG+TGG + chr4.3:795698-795717 MS.gene95877:CDS 40.0%
AATCATGACATGGGGTTTCA+TGG - chr4.3:795020-795039 None:intergenic 40.0%
ACTCCTGTTATCAGTCCAAT+GGG + chr4.3:794752-794771 MS.gene95877:CDS 40.0%
AGCTTATGTGCTTGGGAAAT+TGG + chr4.3:795796-795815 MS.gene95877:CDS 40.0%
AGTAGAATGAAAGGAGGTAG+TGG - chr4.3:794735-794754 None:intergenic 40.0%
ATAGGTGCAAGACTTCTTGA+GGG - chr4.3:795894-795913 None:intergenic 40.0%
ATCATGACATGGGGTTTCAT+GGG - chr4.3:795019-795038 None:intergenic 40.0%
ATCATTCTCTTTCTCGTCGT+TGG + chr4.3:794918-794937 MS.gene95877:intron 40.0%
GAAACCCCATGTCATGATTA+AGG + chr4.3:795021-795040 MS.gene95877:intron 40.0%
GAGTCAAAGATTAGTGAGTG+TGG + chr4.3:795661-795680 MS.gene95877:CDS 40.0%
GGACTTTGAACATAGTATGC+TGG - chr4.3:794692-794711 None:intergenic 40.0%
GTCACTAGATCGAGATTGAA+TGG + chr4.3:795461-795480 MS.gene95877:intron 40.0%
GTGTCAATGTTGTGTATGTC+TGG + chr4.3:795366-795385 MS.gene95877:intron 40.0%
TACTCCTGTTATCAGTCCAA+TGG + chr4.3:794751-794770 MS.gene95877:CDS 40.0%
TAGCACCACTTCCATACAAT+GGG - chr4.3:795706-795725 None:intergenic 40.0%
TAGTAACACCAAACCATGGA+AGG + chr4.3:794847-794866 MS.gene95877:CDS 40.0%
TCAGAAATCGTAATTGGCAC+TGG + chr4.3:795296-795315 MS.gene95877:CDS 40.0%
! AGATCTAGAGCTTATGTGCT+TGG + chr4.3:795788-795807 MS.gene95877:CDS 40.0%
! GATCTAGAGCTTATGTGCTT+GGG + chr4.3:795789-795808 MS.gene95877:CDS 40.0%
! GGTTGTTGTTGTTGAGTTTC+CGG - chr4.3:794823-794842 None:intergenic 40.0%
! GTTGTTGTTGTTGAGTTTCC+GGG - chr4.3:794822-794841 None:intergenic 40.0%
!! TCCATGGTTTGGTGTTACTA+CGG - chr4.3:794847-794866 None:intergenic 40.0%
!!! CGTAACAAGGGCACATTTTT+TGG + chr4.3:795236-795255 MS.gene95877:CDS 40.0%
!!! GTTTTTTCACCATCATGGGA+TGG - chr4.3:794713-794732 None:intergenic 40.0%
ACCGTAGTAACACCAAACCA+TGG + chr4.3:794843-794862 MS.gene95877:CDS 45.0%
ATTAGTGAGTGTGGCAGTGA+TGG + chr4.3:795670-795689 MS.gene95877:CDS 45.0%
CATAGGTGCAAGACTTCTTG+AGG - chr4.3:795895-795914 None:intergenic 45.0%
CTAGAGGAAGAGTGAGAATG+AGG - chr4.3:794785-794804 None:intergenic 45.0%
CTAGCACCACTTCCATACAA+TGG - chr4.3:795707-795726 None:intergenic 45.0%
GAACCCATTGGACTGATAAC+AGG - chr4.3:794758-794777 None:intergenic 45.0%
GAGCATGTTAGAGTTCAAGC+TGG + chr4.3:795149-795168 MS.gene95877:CDS 45.0%
GCATACAAAGACAGACTCAG+AGG + chr4.3:794622-794641 MS.gene95877:CDS 45.0%
GGAAGCAAGATCCCATTGTA+TGG + chr4.3:795692-795711 MS.gene95877:CDS 45.0%
GTTCAAAGTCCATCCCATGA+TGG + chr4.3:794701-794720 MS.gene95877:CDS 45.0%
TTAGTGAGTGTGGCAGTGAT+GGG + chr4.3:795671-795690 MS.gene95877:CDS 45.0%
! TCAAGCTGGTTCAGATGCAA+CGG + chr4.3:795163-795182 MS.gene95877:CDS 45.0%
!! ATGGTTTGGTGTTACTACGG+TGG - chr4.3:794844-794863 None:intergenic 45.0%
ACATAGTATGCTGGTCGACG+AGG - chr4.3:794683-794702 None:intergenic 50.0%
GAATGAGGAGGAGAACCCAT+TGG - chr4.3:794770-794789 None:intergenic 50.0%
GAGGAAGAGTGAGAATGAGG+AGG - chr4.3:794782-794801 None:intergenic 50.0%
GTTTCCGGGAACCAGAGAAA+CGG - chr4.3:794808-794827 None:intergenic 50.0%
TCGACGAGGAGATCTTGTTG+TGG - chr4.3:794669-794688 None:intergenic 50.0%
TCTTCCTCTAGCCGTTTCTC+TGG + chr4.3:794794-794813 MS.gene95877:CDS 50.0%
! CAAGCTGGTTCAGATGCAAC+GGG + chr4.3:795164-795183 MS.gene95877:CDS 50.0%
!! GCTAGTCTTAGCAGCACAAC+TGG + chr4.3:795722-795741 MS.gene95877:CDS 50.0%
GGAACCAGAGAAACGGCTAG+AGG - chr4.3:794801-794820 None:intergenic 55.0%
TGGTTCAGATGCAACGGGTG+TGG + chr4.3:795169-795188 MS.gene95877:CDS 55.0%
! CTAGCCGTTTCTCTGGTTCC+CGG + chr4.3:794801-794820 MS.gene95877:CDS 55.0%
TGGGTCTGCTAGCACCCCAA+AGG - chr4.3:795000-795019 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 794620 795919 794620 ID=MS.gene95877
chr4.3 mRNA 794620 795919 794620 ID=MS.gene95877.t1;Parent=MS.gene95877
chr4.3 exon 794620 794877 794620 ID=MS.gene95877.t1.exon1;Parent=MS.gene95877.t1
chr4.3 CDS 794620 794877 794620 ID=cds.MS.gene95877.t1;Parent=MS.gene95877.t1
chr4.3 exon 795137 795322 795137 ID=MS.gene95877.t1.exon2;Parent=MS.gene95877.t1
chr4.3 CDS 795137 795322 795137 ID=cds.MS.gene95877.t1;Parent=MS.gene95877.t1
chr4.3 exon 795635 795919 795635 ID=MS.gene95877.t1.exon3;Parent=MS.gene95877.t1
chr4.3 CDS 795635 795919 795635 ID=cds.MS.gene95877.t1;Parent=MS.gene95877.t1
Gene Sequence

>MS.gene95877

ATGCATACAAAGACAGACTCAGAGGTTACTAGCTTAGATGCATCATCCACAACAAGATCTCCTCGTCGACCAGCATACTATGTTCAAAGTCCATCCCATGATGGTGAAAAAACCACTACCTCCTTTCATTCTACTCCTGTTATCAGTCCAATGGGTTCTCCTCCTCATTCTCACTCTTCCTCTAGCCGTTTCTCTGGTTCCCGGAAACTCAACAACAACAACCACCGTAGTAACACCAAACCATGGAAGGAAATTGATGTAATTGAAGAAGAAGGTCTTCTTAACAATCAAGATCATCATCATTCTCTTTCTCGTCGTTGGTATTTCCTTGCTTTCATTCTTGGCTTTTTCCTTTTGTTCACTCTTTTCTCTCTTATCCTTTGGGGTGCTAGCAGACCCATGAAACCCCATGTCATGATTAAGGTATACTTTTTATTTTTATTCTGTTTTCTAAATTCTTTTAATTGTTATGTCAAATGGGTTTTAATCAATTTTTGTTTGATTTTGTGTTTTGTAGAGTATAAAATTTGAGCATGTTAGAGTTCAAGCTGGTTCAGATGCAACGGGTGTGGCCACTGATATGATTACAGTGAATGCAACTGTGAAATTCACTTATCGTAACAAGGGCACATTTTTTGGTGTTCATGTCTCATCCACACCTTTAGATCTATCCTATTCAGAAATCGTAATTGGCACTGGCAATGTAAGTCATAAGTTACTTGCTTTTTTTTTTTTTTTTTTTTTATGTGTCAATGTTGTGTATGTCTGGTGTTCTAATAGATACGAATTCAGATTGATTGCAATCGGCAATTCCCGAGTTTACACGATAATCATATTAGTTGTCACTAGATCGAGATTGAATGGTTTAGATTGTGTTCGATCTCTATCTGACAACAATCGATACACTGATTGGAGTCAATCTGAATTCTGATGCACACATTAATTTTTAGTTTGATTTTAATATGAATTACTGAAAATTGTGTATCAATCTTTTTCTTTGGATTTGTGATTGCAGATGAAGAAGTTTTATCAATCAAGGAGGAGTCAAAGATTAGTGAGTGTGGCAGTGATGGGAAGCAAGATCCCATTGTATGGAAGTGGTGCTAGTCTTAGCAGCACAACTGGTATGCCAACTTTACCAGTGCCATTAAAATTGAGCTTTGAGATTAGATCTAGAGCTTATGTGCTTGGGAAATTGGTCAAACCAAAATACTACAAAAAGGTTCATTGTTCTGTCGTATTTGATCCCAAGAAAATCAATGTTTCTGTTTCCCTCAAGAAGTCTTGCACCTATGATTGA

Protein sequence

>MS.gene95877.t1

MHTKTDSEVTSLDASSTTRSPRRPAYYVQSPSHDGEKTTTSFHSTPVISPMGSPPHSHSSSSRFSGSRKLNNNNHRSNTKPWKEIDSIKFEHVRVQAGSDATGVATDMITVNATVKFTYRNKGTFFGVHVSSTPLDLSYSEIVIGTGNMKKFYQSRRSQRLVSVAVMGSKIPLYGSGASLSSTTGMPTLPVPLKLSFEIRSRAYVLGKLVKPKYYKKVHCSVVFDPKKINVSVSLKKSCTYD