AlfalfaGEDB | MS.gene96021

M. sativa cultivar XinJiangDaYe / MS.gene96021

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF00197.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96021.t1	XP_013464493.1	83.4	205	28	1	1	205	1	199	3.70E-91	344.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96021.t1	P29421	30.3	165	111	1	11	171	6	170	1.6e-13	77.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96021.t1	G8A259	83.4	205	28	1	1	205	1	199	2.7e-91	344.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051789	MS.gene96021	0.816546	5.23E-52	-1.69E-46
MS.gene052142	MS.gene96021	0.818245	2.17E-52	-1.69E-46
MS.gene052187	MS.gene96021	0.805949	1.04E-49	-1.69E-46
MS.gene053074	MS.gene96021	0.863697	2.07E-64	-1.69E-46
MS.gene053383	MS.gene96021	0.808382	3.18E-50	-1.69E-46
MS.gene054596	MS.gene96021	0.822475	2.32E-53	-1.69E-46
MS.gene055359	MS.gene96021	0.839403	1.62E-57	-1.69E-46
MS.gene058168	MS.gene96021	0.833304	5.76E-56	-1.69E-46
MS.gene059006	MS.gene96021	0.842556	2.41E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96021.t1	MTR_2g072980	83.415	205	28	1	1	205	1	199	2.08e-120	339
MS.gene96021.t1	MTR_2g073010	65.714	210	55	4	1	205	1	198	3.48e-89	260
MS.gene96021.t1	MTR_8g059790	47.291	203	83	6	1	188	1	194	2.79e-52	167
MS.gene96021.t1	MTR_0211s0080	45.960	198	87	6	1	188	1	188	1.23e-48	157
MS.gene96021.t1	MTR_0100s0150	44.776	201	88	6	1	188	1	191	3.94e-48	156
MS.gene96021.t1	MTR_8g461050	43.564	202	90	6	1	188	1	192	3.33e-46	151
MS.gene96021.t1	MTR_0302s0030	43.069	202	91	6	1	188	1	192	3.40e-46	151
MS.gene96021.t1	MTR_3g014820	42.365	203	92	6	1	188	1	193	1.63e-45	150
MS.gene96021.t1	MTR_3g014870	42.365	203	92	6	1	188	1	193	4.45e-44	146
MS.gene96021.t1	MTR_0084s0030	43.069	202	91	6	1	188	1	192	1.13e-43	145
MS.gene96021.t1	MTR_7g108815	43.069	202	89	8	1	188	1	190	6.32e-42	140
MS.gene96021.t1	MTR_8g060550	41.176	204	88	8	15	204	14	199	9.58e-42	140
MS.gene96021.t1	MTR_8g461690	41.176	204	88	8	15	204	14	199	9.58e-42	140
MS.gene96021.t1	MTR_6g078070	32.727	165	103	5	14	173	10	171	6.91e-18	78.6
MS.gene96021.t1	MTR_6g078100	30.732	205	120	8	14	205	10	205	5.23e-16	73.6
MS.gene96021.t1	MTR_6g078290	31.100	209	108	9	8	189	4	203	6.01e-13	65.5
MS.gene96021.t1	MTR_6g066210	30.055	183	102	8	8	174	4	176	2.10e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96021.t1	AT1G17860	29.187	209	120	8	11	205	1	195	5.65e-14	67.8

Find 52 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGGTAATTTGCATGAAAA+TGG	0.244897	2.4:+23247872	MS.gene96021:CDS
ACAATCTACGGAATGGAAAT+TGG	0.250660	2.4:+23247697	MS.gene96021:CDS
AGTGACACGGCCTCCGATAT+CGG	0.290496	2.4:-23247513	None:intergenic
GTTTCTTGACCCACATATAA+AGG	0.297981	2.4:-23247560	None:intergenic
CGGAATGGAAATTGGGTGAT+AGG	0.325123	2.4:+23247705	MS.gene96021:CDS
ATACAGTTGTTAAGGTGAAT+AGG	0.332362	2.4:+23247636	MS.gene96021:CDS
CAAGCCTTAGCCTTATGTTA+TGG	0.345426	2.4:+23247381	MS.gene96021:CDS
GGTCAAGAAACTACAGATAT+TGG	0.348677	2.4:+23247572	MS.gene96021:CDS
TACAGTTGTTAAGGTGAATA+GGG	0.357658	2.4:+23247637	MS.gene96021:CDS
AAGACTCCACTTGGAATTGA+AGG	0.410273	2.4:+23247791	MS.gene96021:CDS
AAGGTTAATGTTCTCAAGGT+TGG	0.412184	2.4:+23247935	MS.gene96021:CDS
AGGTTAATGTTCTCAAGGTT+GGG	0.412594	2.4:+23247936	MS.gene96021:CDS
AACAGAAGATACAGTTGTTA+AGG	0.418077	2.4:+23247628	MS.gene96021:CDS
CGGTTCATGCCCTTTATATG+TGG	0.431349	2.4:+23247550	MS.gene96021:CDS
CTGATCTTATGTAATACTTC+CGG	0.434280	2.4:-23247484	None:intergenic
TTTCTTGACCCACATATAAA+GGG	0.438692	2.4:-23247559	None:intergenic
AATTGGGTGATAGGGACAAT+AGG	0.441749	2.4:+23247714	MS.gene96021:CDS
GTGACACGGCCTCCGATATC+GGG	0.449326	2.4:-23247512	None:intergenic
CAATCTACGGAATGGAAATT+GGG	0.450660	2.4:+23247698	MS.gene96021:CDS
TTCAAGTTGTGGACAATCTA+CGG	0.461337	2.4:+23247685	MS.gene96021:CDS
AGATCAGATGTACCCGATAT+CGG	0.472186	2.4:+23247500	MS.gene96021:CDS
TTTGATCAATCTTGTTGTCA+TGG	0.481185	2.4:-23247354	None:intergenic
AGGTTGTGCTAATGTTGGTA+TGG	0.501409	2.4:-23247414	None:intergenic
TCATGAAAAGCCATAACATA+AGG	0.507153	2.4:-23247391	None:intergenic
ACGGTTAATTGCGGTACGGT+TGG	0.512688	2.4:+23247854	MS.gene96021:CDS
GGAAGGAGGCTTATTACAGC+GGG	0.513610	2.4:+23247740	MS.gene96021:CDS
GTCACTTTGATAAATAGAAA+CGG	0.522150	2.4:+23247530	MS.gene96021:CDS
GGTGATAGGGACAATAGGAA+TGG	0.534005	2.4:+23247719	MS.gene96021:CDS
GGAATGGAAATTGGGTGATA+GGG	0.534497	2.4:+23247706	MS.gene96021:CDS
GTCGTCCTCTTGAGAGTGGC+CGG	0.536921	2.4:+23247465	MS.gene96021:CDS
CTTATTACAGCGGGGAGTGA+TGG	0.543645	2.4:+23247749	MS.gene96021:CDS
TGGTATTAATAATGTTCTCA+AGG	0.548464	2.4:+23247916	MS.gene96021:CDS
TCTCAAGGTTAATGTTCTCA+AGG	0.553729	2.4:+23247931	MS.gene96021:CDS
ACTGTATCTTCTGTTGCCAT+AGG	0.571927	2.4:-23247620	None:intergenic
ATAGGGACAATAGGAATGGA+AGG	0.576527	2.4:+23247723	MS.gene96021:CDS
GGTTCATGCCCTTTATATGT+GGG	0.576927	2.4:+23247551	MS.gene96021:CDS
ACCGCAATTAACCGTTTCCG+AGG	0.595491	2.4:-23247846	None:intergenic
GTTGTGGACAATCTACGGAA+TGG	0.597660	2.4:+23247690	MS.gene96021:CDS
AAAGCCATAACATAAGGCTA+AGG	0.601835	2.4:-23247385	None:intergenic
TGGAAGGAGGCTTATTACAG+CGG	0.611644	2.4:+23247739	MS.gene96021:CDS
TACTTCCGGCCACTCTCAAG+AGG	0.616975	2.4:-23247470	None:intergenic
TTCTATTTATCAAAGTGACA+CGG	0.619288	2.4:-23247526	None:intergenic
ACACGTCGTCCTCTTGAGAG+TGG	0.635863	2.4:+23247461	MS.gene96021:CDS
CCGCAATTAACCGTTTCCGA+GGG	0.637522	2.4:-23247845	None:intergenic
AAAACACCTTCAATTCCAAG+TGG	0.642111	2.4:-23247797	None:intergenic
CCCTCGGAAACGGTTAATTG+CGG	0.663767	2.4:+23247845	MS.gene96021:CDS
GAAACTACAGATATTGGACA+AGG	0.666365	2.4:+23247578	MS.gene96021:CDS
GGAAACGGTTAATTGCGGTA+CGG	0.670786	2.4:+23247850	MS.gene96021:CDS
TCAGATGTACCCGATATCGG+AGG	0.696816	2.4:+23247503	MS.gene96021:CDS
GGGACAATAGGAATGGAAGG+AGG	0.712776	2.4:+23247726	MS.gene96021:CDS
AAACTACAGATATTGGACAA+GGG	0.717751	2.4:+23247579	MS.gene96021:CDS
GAAGGAGGCTTATTACAGCG+GGG	0.754937	2.4:+23247741	MS.gene96021:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTAGGTAATTATAATATT+AGG	+	chr2.4:23247816-23247835	MS.gene96021:CDS	10.0%
!!!	TGAAAATGGTAAGATTTTAT+TGG	+	chr2.4:23247886-23247905	MS.gene96021:CDS	20.0%
!	TGGTATTAATAATGTTCTCA+AGG	+	chr2.4:23247916-23247935	MS.gene96021:CDS	25.0%
!	TTCTATTTATCAAAGTGACA+CGG	-	chr2.4:23247529-23247548	None:intergenic	25.0%
!!	GTCACTTTGATAAATAGAAA+CGG	+	chr2.4:23247530-23247549	MS.gene96021:CDS	25.0%
!!!	ATCAGTTATTTTTACTCCTA+TGG	+	chr2.4:23247604-23247623	MS.gene96021:CDS	25.0%
	AAACTACAGATATTGGACAA+GGG	+	chr2.4:23247579-23247598	MS.gene96021:CDS	30.0%
	AACAGAAGATACAGTTGTTA+AGG	+	chr2.4:23247628-23247647	MS.gene96021:CDS	30.0%
	ATACAGTTGTTAAGGTGAAT+AGG	+	chr2.4:23247636-23247655	MS.gene96021:CDS	30.0%
	CTGATCTTATGTAATACTTC+CGG	-	chr2.4:23247487-23247506	None:intergenic	30.0%
	TACAGTTGTTAAGGTGAATA+GGG	+	chr2.4:23247637-23247656	MS.gene96021:CDS	30.0%
	TCATGAAAAGCCATAACATA+AGG	-	chr2.4:23247394-23247413	None:intergenic	30.0%
	TTTCTTGACCCACATATAAA+GGG	-	chr2.4:23247562-23247581	None:intergenic	30.0%
!	TAAGGTGAATAGGGATTTTA+AGG	+	chr2.4:23247646-23247665	MS.gene96021:CDS	30.0%
!	TCAAGAACTTCTTCATTTTC+AGG	-	chr2.4:23247437-23247456	None:intergenic	30.0%
!!	GTTGGTAATTTGCATGAAAA+TGG	+	chr2.4:23247872-23247891	MS.gene96021:CDS	30.0%
!!	TAATATTAGGTTTTGTCCCT+CGG	+	chr2.4:23247829-23247848	MS.gene96021:CDS	30.0%
!!!	AAGATTTTATTGGCTTTGGA+TGG	+	chr2.4:23247896-23247915	MS.gene96021:CDS	30.0%
!!!	TGGTAAGATTTTATTGGCTT+TGG	+	chr2.4:23247892-23247911	MS.gene96021:CDS	30.0%
	AAAACACCTTCAATTCCAAG+TGG	-	chr2.4:23247800-23247819	None:intergenic	35.0%
	AAAGCCATAACATAAGGCTA+AGG	-	chr2.4:23247388-23247407	None:intergenic	35.0%
	AAGGTTAATGTTCTCAAGGT+TGG	+	chr2.4:23247935-23247954	MS.gene96021:CDS	35.0%
	ACAATCTACGGAATGGAAAT+TGG	+	chr2.4:23247697-23247716	MS.gene96021:CDS	35.0%
	AGGTTAATGTTCTCAAGGTT+GGG	+	chr2.4:23247936-23247955	MS.gene96021:CDS	35.0%
	CAATCTACGGAATGGAAATT+GGG	+	chr2.4:23247698-23247717	MS.gene96021:CDS	35.0%
	GAAACTACAGATATTGGACA+AGG	+	chr2.4:23247578-23247597	MS.gene96021:CDS	35.0%
	GGTCAAGAAACTACAGATAT+TGG	+	chr2.4:23247572-23247591	MS.gene96021:CDS	35.0%
	GTTTCTTGACCCACATATAA+AGG	-	chr2.4:23247563-23247582	None:intergenic	35.0%
	TCTCAAGGTTAATGTTCTCA+AGG	+	chr2.4:23247931-23247950	MS.gene96021:CDS	35.0%
!!	AGAATTTTGAAGACTCCACT+TGG	+	chr2.4:23247782-23247801	MS.gene96021:CDS	35.0%
!!	TTCAAGTTGTGGACAATCTA+CGG	+	chr2.4:23247685-23247704	MS.gene96021:CDS	35.0%
!!!	CTTGGAATTGAAGGTGTTTT+AGG	+	chr2.4:23247800-23247819	MS.gene96021:CDS	35.0%
!!!	TTTTCAGCTTCTTCAAGTTG+TGG	+	chr2.4:23247674-23247693	MS.gene96021:CDS	35.0%
!!!	TTTTCAGGTTGTGCTAATGT+TGG	-	chr2.4:23247422-23247441	None:intergenic	35.0%
	AAGACTCCACTTGGAATTGA+AGG	+	chr2.4:23247791-23247810	MS.gene96021:CDS	40.0%
	AATTGGGTGATAGGGACAAT+AGG	+	chr2.4:23247714-23247733	MS.gene96021:CDS	40.0%
	ACTGTATCTTCTGTTGCCAT+AGG	-	chr2.4:23247623-23247642	None:intergenic	40.0%
	AGATCAGATGTACCCGATAT+CGG	+	chr2.4:23247500-23247519	MS.gene96021:CDS	40.0%
	AGGTTGTGCTAATGTTGGTA+TGG	-	chr2.4:23247417-23247436	None:intergenic	40.0%
	ATAGGGACAATAGGAATGGA+AGG	+	chr2.4:23247723-23247742	MS.gene96021:CDS	40.0%
	GGAATGGAAATTGGGTGATA+GGG	+	chr2.4:23247706-23247725	MS.gene96021:CDS	40.0%
	GGTTCATGCCCTTTATATGT+GGG	+	chr2.4:23247551-23247570	MS.gene96021:CDS	40.0%
!!	CAAGCCTTAGCCTTATGTTA+TGG	+	chr2.4:23247381-23247400	MS.gene96021:CDS	40.0%
	CGGAATGGAAATTGGGTGAT+AGG	+	chr2.4:23247705-23247724	MS.gene96021:CDS	45.0%
	CGGTTCATGCCCTTTATATG+TGG	+	chr2.4:23247550-23247569	MS.gene96021:CDS	45.0%
	GGAAACGGTTAATTGCGGTA+CGG	+	chr2.4:23247850-23247869	MS.gene96021:CDS	45.0%
	GGTGATAGGGACAATAGGAA+TGG	+	chr2.4:23247719-23247738	MS.gene96021:CDS	45.0%
	GTTGTGGACAATCTACGGAA+TGG	+	chr2.4:23247690-23247709	MS.gene96021:CDS	45.0%
	TGGAAGGAGGCTTATTACAG+CGG	+	chr2.4:23247739-23247758	MS.gene96021:CDS	45.0%
!!	TAGGTTTTGTCCCTCGGAAA+CGG	+	chr2.4:23247835-23247854	MS.gene96021:CDS	45.0%
	ACCGCAATTAACCGTTTCCG+AGG	-	chr2.4:23247849-23247868	None:intergenic	50.0%
	ACGGTTAATTGCGGTACGGT+TGG	+	chr2.4:23247854-23247873	MS.gene96021:CDS	50.0%
	CCCTCGGAAACGGTTAATTG+CGG	+	chr2.4:23247845-23247864	MS.gene96021:CDS	50.0%
	CCGCAATTAACCGTTTCCGA+GGG	-	chr2.4:23247848-23247867	None:intergenic	50.0%
	CTTATTACAGCGGGGAGTGA+TGG	+	chr2.4:23247749-23247768	MS.gene96021:CDS	50.0%
	GAAGGAGGCTTATTACAGCG+GGG	+	chr2.4:23247741-23247760	MS.gene96021:CDS	50.0%
	GGAAGGAGGCTTATTACAGC+GGG	+	chr2.4:23247740-23247759	MS.gene96021:CDS	50.0%
	GGGACAATAGGAATGGAAGG+AGG	+	chr2.4:23247726-23247745	MS.gene96021:CDS	50.0%
	TCAGATGTACCCGATATCGG+AGG	+	chr2.4:23247503-23247522	MS.gene96021:CDS	50.0%
	ACACGTCGTCCTCTTGAGAG+TGG	+	chr2.4:23247461-23247480	MS.gene96021:CDS	55.0%
	AGTGACACGGCCTCCGATAT+CGG	-	chr2.4:23247516-23247535	None:intergenic	55.0%
	TACTTCCGGCCACTCTCAAG+AGG	-	chr2.4:23247473-23247492	None:intergenic	55.0%
	GTCGTCCTCTTGAGAGTGGC+CGG	+	chr2.4:23247465-23247484	MS.gene96021:CDS	60.0%
	GTGACACGGCCTCCGATATC+GGG	-	chr2.4:23247515-23247534	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	23247356	23247973	23247356	ID=MS.gene96021
chr2.4	mRNA	23247356	23247973	23247356	ID=MS.gene96021.t1;Parent=MS.gene96021
chr2.4	exon	23247356	23247973	23247356	ID=MS.gene96021.t1.exon1;Parent=MS.gene96021.t1
chr2.4	CDS	23247356	23247973	23247356	ID=cds.MS.gene96021.t1;Parent=MS.gene96021.t1

Gene Sequence

>MS.gene96021

ATGACAACAAGATTGATCAAAATCACAAGCCTTAGCCTTATGTTATGGCTTTTCATGACCATACCAACATTAGCACAACCTGAAAATGAAGAAGTTCTTGACTTAACACGTCGTCCTCTTGAGAGTGGCCGGAAGTATTACATAAGATCAGATGTACCCGATATCGGAGGCCGTGTCACTTTGATAAATAGAAACGGTTCATGCCCTTTATATGTGGGTCAAGAAACTACAGATATTGGACAAGGGTTATCAGTTATTTTTACTCCTATGGCAACAGAAGATACAGTTGTTAAGGTGAATAGGGATTTTAAGGTGAAATTTTCAGCTTCTTCAAGTTGTGGACAATCTACGGAATGGAAATTGGGTGATAGGGACAATAGGAATGGAAGGAGGCTTATTACAGCGGGGAGTGATGGTAGTTATTTTAGAATTTTGAAGACTCCACTTGGAATTGAAGGTGTTTTAGGTAATTATAATATTAGGTTTTGTCCCTCGGAAACGGTTAATTGCGGTACGGTTGGTAATTTGCATGAAAATGGTAAGATTTTATTGGCTTTGGATGGTATTAATAATGTTCTCAAGGTTAATGTTCTCAAGGTTGGGTTCGAGAGAGCGTAA

Protein sequence

>MS.gene96021.t1

MTTRLIKITSLSLMLWLFMTIPTLAQPENEEVLDLTRRPLESGRKYYIRSDVPDIGGRVTLINRNGSCPLYVGQETTDIGQGLSVIFTPMATEDTVVKVNRDFKVKFSASSSCGQSTEWKLGDRDNRNGRRLITAGSDGSYFRILKTPLGIEGVLGNYNIRFCPSETVNCGTVGNLHENGKILLALDGINNVLKVNVLKVGFERA