AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96275

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96275.t1 MTR_2g018750 97.794 408 9 0 2 409 43 450 0.0 828
MS.gene96275.t1 MTR_1g064360 69.024 410 98 4 2 406 43 428 0.0 570
MS.gene96275.t1 MTR_2g048390 56.039 414 171 7 2 407 43 453 2.52e-159 457
MS.gene96275.t1 MTR_1g094850 52.580 407 185 5 2 406 43 443 4.59e-143 421
MS.gene96275.t1 MTR_8g078900 40.278 432 228 10 2 407 37 464 7.28e-102 311
MS.gene96275.t1 MTR_8g078900 42.820 383 207 8 33 407 46 424 3.90e-101 307
MS.gene96275.t1 MTR_8g078900 40.509 432 227 11 2 407 7 434 5.83e-99 308
MS.gene96275.t1 MTR_8g078900 40.046 432 229 10 2 407 37 464 1.20e-98 308
MS.gene96275.t1 MTR_2g084595 37.947 419 235 5 2 407 40 446 1.28e-98 302
MS.gene96275.t1 MTR_8g078900 42.559 383 208 8 33 407 46 424 7.22e-98 305
MS.gene96275.t1 MTR_2g084595 37.209 430 241 6 2 418 40 453 6.53e-96 300
MS.gene96275.t1 MTR_2g009340 39.683 441 226 8 2 406 61 497 1.67e-95 301
MS.gene96275.t1 MTR_4g093850 39.498 438 226 8 2 407 61 491 4.47e-94 293
MS.gene96275.t1 MTR_4g093850 39.269 438 227 8 2 407 61 491 5.87e-92 291
MS.gene96275.t1 MTR_7g073980 36.429 420 229 10 2 410 50 442 2.29e-80 260
MS.gene96275.t1 MTR_4g022330 36.145 415 230 9 2 406 51 440 8.70e-80 258
MS.gene96275.t1 MTR_5g032780 37.385 436 234 9 2 407 43 469 1.05e-78 256
MS.gene96275.t1 MTR_1g014070 37.805 410 234 10 2 406 44 437 2.65e-78 254
MS.gene96275.t1 MTR_3g062540 35.885 418 208 7 2 407 41 410 5.05e-78 252
MS.gene96275.t1 MTR_3g062540 35.904 415 206 7 5 407 2 368 1.33e-77 250
MS.gene96275.t1 MTR_5g016060 35.109 413 233 7 2 406 57 442 1.56e-77 253
MS.gene96275.t1 MTR_5g075640 33.890 419 230 6 2 410 42 423 1.49e-72 238
MS.gene96275.t1 MTR_8g090220 34.247 438 217 11 2 410 69 464 1.67e-72 236
MS.gene96275.t1 MTR_5g075640 33.894 416 228 6 5 410 2 380 3.10e-72 236
MS.gene96275.t1 MTR_1g106430 35.122 410 244 8 2 406 45 437 9.41e-72 237
MS.gene96275.t1 MTR_8g090220 34.247 438 217 11 2 410 69 464 2.05e-71 236
MS.gene96275.t1 MTR_4g095430 34.063 411 239 9 2 406 30 414 2.28e-70 233
MS.gene96275.t1 MTR_5g015790 36.675 409 235 11 2 406 44 432 2.68e-69 231
MS.gene96275.t1 MTR_4g134210 33.333 408 245 6 2 407 43 425 2.56e-65 219
MS.gene96275.t1 MTR_1g088885 33.732 418 239 10 2 407 53 444 7.88e-64 216
MS.gene96275.t1 MTR_7g108290 35.154 421 239 11 2 410 53 451 8.98e-64 217
MS.gene96275.t1 MTR_8g069330 34.404 436 210 13 2 406 43 433 1.54e-61 209
MS.gene96275.t1 MTR_1g082690 30.435 414 221 8 2 406 75 430 1.65e-60 206
MS.gene96275.t1 MTR_2g027410 32.847 411 233 8 4 409 45 417 8.86e-60 204
MS.gene96275.t1 MTR_5g016060 33.239 352 202 6 63 406 2 328 6.68e-56 192
MS.gene96275.t1 MTR_2g027410 32.283 381 217 7 34 409 1 345 3.24e-53 185
MS.gene96275.t1 MTR_4g045767 28.571 413 234 14 9 408 29 393 1.28e-39 149
MS.gene96275.t1 MTR_4g074190 26.733 404 248 11 29 414 49 422 1.77e-38 144
MS.gene96275.t1 MTR_4g011530 32.661 248 146 3 165 410 38 266 4.86e-37 140
MS.gene96275.t1 MTR_2g102730 35.000 240 137 2 173 410 24 246 8.10e-37 139
MS.gene96275.t1 MTR_4g074190 26.238 404 250 11 29 414 3 376 9.55e-37 140
MS.gene96275.t1 MTR_1g035720 25.943 424 243 13 10 406 29 408 1.18e-36 140
MS.gene96275.t1 MTR_4g074190 26.238 404 250 11 29 414 49 422 1.84e-36 140
MS.gene96275.t1 MTR_1g105870 32.292 192 111 4 2 192 48 221 5.33e-27 108
MS.gene96275.t1 MTR_2g010890 27.100 369 212 11 53 409 1 324 2.22e-22 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96275.t1 AT5G03250 63.504 411 142 5 2 406 43 451 0.0 537
MS.gene96275.t1 AT5G03250 61.584 341 123 5 72 406 1 339 4.43e-147 427
MS.gene96275.t1 AT1G30440 50.481 416 194 7 2 406 43 457 5.10e-144 426
MS.gene96275.t1 AT5G13600 53.828 418 166 11 2 408 43 444 7.10e-138 407
MS.gene96275.t1 AT3G44820 39.770 435 216 9 2 406 40 458 1.02e-98 308
MS.gene96275.t1 AT5G66560 42.564 390 199 10 29 407 105 480 2.16e-97 300
MS.gene96275.t1 AT5G66560 42.308 390 200 10 29 407 66 441 4.08e-95 298
MS.gene96275.t1 AT5G66560 42.308 390 200 10 29 407 76 451 1.19e-94 297
MS.gene96275.t1 AT5G66560 42.308 390 200 10 29 407 105 480 1.48e-94 298
MS.gene96275.t1 AT5G48800 38.835 412 211 8 2 407 58 434 8.99e-83 266
MS.gene96275.t1 AT3G50840 38.942 416 209 11 2 409 33 411 3.39e-82 263
MS.gene96275.t1 AT3G50840 38.835 412 207 11 6 409 1 375 1.70e-79 255
MS.gene96275.t1 AT3G50840 38.835 412 207 11 6 409 1 375 1.70e-79 255
MS.gene96275.t1 AT3G08570 37.198 414 226 9 2 406 51 439 4.06e-77 251
MS.gene96275.t1 AT5G67385 35.049 408 236 8 2 406 42 423 7.81e-77 250
MS.gene96275.t1 AT5G67385 34.653 404 235 8 6 406 1 378 2.86e-74 242
MS.gene96275.t1 AT5G67385 34.653 404 235 8 6 406 1 378 2.86e-74 242
MS.gene96275.t1 AT5G67385 34.653 404 235 8 6 406 1 378 2.86e-74 242
MS.gene96275.t1 AT5G67385 34.653 404 235 8 6 406 1 378 2.86e-74 242
MS.gene96275.t1 AT2G30520 34.634 410 225 9 2 406 47 418 1.92e-72 238
MS.gene96275.t1 AT3G08660 34.689 418 219 9 2 412 51 421 2.58e-72 233
MS.gene96275.t1 AT2G30520 34.644 407 223 9 5 406 2 370 5.32e-72 236
MS.gene96275.t1 AT2G30520 34.644 407 223 9 5 406 2 370 5.32e-72 236
MS.gene96275.t1 AT1G67900 35.731 431 238 8 2 407 43 459 8.41e-71 235
MS.gene96275.t1 AT1G67900 35.731 431 238 8 2 407 43 459 8.41e-71 235
MS.gene96275.t1 AT1G67900 35.731 431 238 8 2 407 43 459 8.41e-71 235
MS.gene96275.t1 AT1G67900 35.731 431 238 8 2 407 43 459 8.41e-71 235
MS.gene96275.t1 AT1G67900 35.731 431 238 8 2 407 43 459 8.41e-71 235
MS.gene96275.t1 AT1G67900 35.731 431 238 8 2 407 43 459 8.41e-71 235
MS.gene96275.t1 AT3G08660 34.709 412 215 9 2 406 51 415 8.34e-70 231
MS.gene96275.t1 AT1G03010 35.363 427 229 12 2 407 26 426 1.94e-68 228
MS.gene96275.t1 AT1G03010 35.363 427 229 12 2 407 52 452 2.13e-68 228
MS.gene96275.t1 AT1G03010 36.215 428 224 13 2 407 53 453 3.93e-68 228
MS.gene96275.t1 AT5G67440 34.307 411 235 11 2 406 7 388 7.63e-68 225
MS.gene96275.t1 AT2G47860 34.198 424 230 11 1 407 76 467 2.89e-67 221
MS.gene96275.t1 AT4G37590 34.606 419 212 11 2 406 44 414 3.51e-67 224
MS.gene96275.t1 AT5G67440 34.063 411 236 11 2 406 44 425 5.86e-67 223
MS.gene96275.t1 AT5G67440 34.063 411 236 11 2 406 44 425 5.86e-67 223
MS.gene96275.t1 AT2G47860 34.043 423 230 11 2 407 54 444 1.76e-66 219
MS.gene96275.t1 AT2G14820 35.194 412 242 11 2 406 44 437 2.63e-66 223
MS.gene96275.t1 AT2G14820 35.194 412 242 11 2 406 44 437 2.63e-66 223
MS.gene96275.t1 AT3G08660 34.961 389 202 8 25 406 11 355 1.55e-65 218
MS.gene96275.t1 AT2G47860 34.198 424 230 11 1 407 76 467 1.65e-65 221
MS.gene96275.t1 AT5G10250 32.339 436 221 9 2 410 67 455 5.22e-65 219
MS.gene96275.t1 AT2G47860 34.043 423 230 11 2 407 54 444 8.58e-65 219
MS.gene96275.t1 AT3G49970 32.187 407 215 9 2 406 11 358 1.06e-63 213
MS.gene96275.t1 AT3G49970 32.187 407 215 9 2 406 11 358 1.06e-63 213
MS.gene96275.t1 AT3G26490 34.783 437 238 14 2 410 43 460 4.74e-63 211
MS.gene96275.t1 AT4G31820 32.847 411 233 11 2 406 44 417 7.93e-63 212
MS.gene96275.t1 AT3G26490 35.023 434 235 14 2 407 43 457 7.27e-62 210
MS.gene96275.t1 AT3G26490 35.023 434 235 14 2 407 43 457 7.27e-62 210
MS.gene96275.t1 AT3G26490 35.023 434 235 14 2 407 43 457 7.27e-62 210
MS.gene96275.t1 AT5G10250 32.160 426 220 9 2 410 67 440 1.16e-60 207
MS.gene96275.t1 AT2G23050 32.367 414 219 10 2 409 44 402 7.77e-60 202
MS.gene96275.t1 AT4G31820 32.170 401 229 11 12 406 1 364 1.70e-58 199
MS.gene96275.t1 AT5G47800 31.540 409 246 9 5 407 46 426 1.73e-57 198
MS.gene96275.t1 AT5G47800 31.540 409 246 9 5 407 46 426 1.73e-57 198
MS.gene96275.t1 AT5G47800 31.540 409 246 9 5 407 46 426 1.73e-57 198
MS.gene96275.t1 AT5G47800 31.540 409 246 9 5 407 159 539 1.01e-56 198
MS.gene96275.t1 AT5G64330 41.200 250 126 4 2 237 10 252 1.59e-53 188
MS.gene96275.t1 AT5G64330 42.473 186 93 1 235 406 310 495 3.44e-34 134
MS.gene96275.t1 AT5G64330 41.600 250 125 4 2 237 69 311 1.73e-52 186
MS.gene96275.t1 AT5G64330 42.473 186 93 1 235 406 369 554 4.72e-34 134
MS.gene96275.t1 AT5G64330 41.600 250 125 4 2 237 69 311 5.92e-52 186
MS.gene96275.t1 AT5G64330 42.246 187 94 1 235 407 369 555 4.66e-34 135
MS.gene96275.t1 AT3G19850 28.469 418 248 10 9 406 28 414 2.41e-42 157
MS.gene96275.t1 AT3G22104 28.537 410 233 10 9 409 29 387 5.08e-41 152
MS.gene96275.t1 AT2G47860 31.445 353 198 10 72 407 1 326 2.95e-40 150
MS.gene96275.t1 AT2G47860 31.445 353 198 10 72 407 1 326 2.95e-40 150
MS.gene96275.t1 AT1G50280 27.251 422 263 9 9 413 28 422 7.33e-36 138
MS.gene96275.t1 AT1G50280 27.569 399 248 8 29 413 18 389 9.26e-36 137
MS.gene96275.t1 AT2G23050 29.619 341 182 9 72 409 1 286 2.15e-34 131
MS.gene96275.t1 AT5G17580 28.278 389 225 9 29 406 46 391 1.20e-33 132
MS.gene96275.t1 AT1G52770 32.510 243 139 3 173 407 28 253 1.52e-33 131
MS.gene96275.t1 AT5G17580 28.278 389 225 9 29 406 61 406 1.75e-33 132
MS.gene96275.t1 AT3G15570 33.725 255 137 5 168 407 21 258 4.07e-33 129
MS.gene96275.t1 AT3G03510 28.495 372 191 9 41 406 8 310 1.73e-32 128
MS.gene96275.t1 AT3G22104 27.019 359 204 8 59 409 4 312 5.06e-28 115
MS.gene96275.t1 AT3G22104 27.019 359 204 8 59 409 4 312 5.06e-28 115
MS.gene96275.t1 AT3G22104 27.019 359 204 8 59 409 4 312 5.06e-28 115
MS.gene96275.t1 AT5G48130 23.585 424 246 12 5 410 59 422 3.51e-25 108

Find 84 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATCTTCTCATTCCGAATA+TGG 0.185813 2.2:-65739869 MS.gene96275:CDS
TCTAAACCTGTCTAAATTTC+AGG 0.215961 2.2:-65739638 MS.gene96275:CDS
TCTCCATGTATAACTGAAAT+TGG 0.237203 2.2:-65739754 MS.gene96275:CDS
AGATTGTATTCAGAGGATTT+TGG 0.237388 2.2:-65739818 MS.gene96275:CDS
GCACGTTTCAAGCGCTTTGA+TGG 0.261050 2.2:+65740461 None:intergenic
TGTTATGAGCTCAAATGTTT+TGG 0.294973 2.2:+65740644 None:intergenic
TATGGTGCAGATAATCTTAT+TGG 0.306224 2.2:-65740531 MS.gene96275:CDS
TTCTAGATGCATTGATTCCT+TGG 0.328608 2.2:-65740400 MS.gene96275:CDS
CACAGCCTGTTGGTGATAAT+TGG 0.336858 2.2:-65740263 MS.gene96275:CDS
CGGATCCAGATTTGTTCAAT+TGG 0.351033 2.2:-65740362 MS.gene96275:CDS
CAATGCATCTAGAAACAATA+TGG 0.362633 2.2:+65740409 None:intergenic
GAAATTGGAACATTAATTGC+TGG 0.374348 2.2:-65739739 MS.gene96275:CDS
GATGTGACACCTCTCTTATT+AGG 0.375205 2.2:+65740006 None:intergenic
TTGCAACTTCATGATGTTCC+TGG 0.376919 2.2:-65740672 MS.gene96275:CDS
CTTGGCTATGAAGGCTTGTT+CGG 0.381268 2.2:-65740382 MS.gene96275:CDS
TCAAGCGCTTTGATGGAATC+TGG 0.381679 2.2:+65740468 None:intergenic
ACTTGCCAATTGAACAAATC+TGG 0.382711 2.2:+65740357 None:intergenic
CATTGATTCCTTGGCTATGA+AGG 0.384124 2.2:-65740391 MS.gene96275:CDS
TCTAAATTCTCTTTGCATGA+TGG 0.393396 2.2:+65739931 None:intergenic
GCTGGTAGATAGACATAAAA+TGG 0.403847 2.2:+65739794 None:intergenic
CAAAGAGAATTTAGAGAAAA+GGG 0.406093 2.2:-65739923 MS.gene96275:CDS
TGATCAGCTTCAGAAGTTGT+AGG 0.408703 2.2:+65740063 None:intergenic
GCAAAGAGAATTTAGAGAAA+AGG 0.413793 2.2:-65739924 MS.gene96275:CDS
CGCAATAGATGTATACCTTA+AGG 0.419410 2.2:-65739560 MS.gene96275:CDS
TCAAGGGGCCTGGCATAATC+AGG 0.421029 2.2:+65739598 None:intergenic
CATCTCTTATATATTACCTT+AGG 0.423118 2.2:-65740146 MS.gene96275:CDS
CTCCAAATGACTGAAAACTA+TGG 0.440150 2.2:-65740549 MS.gene96275:CDS
TTGCAATTCCTGATTATGCC+AGG 0.447101 2.2:-65739606 MS.gene96275:CDS
TTGTTCAATTGGCAAGTGAC+TGG 0.447348 2.2:-65740351 MS.gene96275:CDS
TGAATCGAAAGGAATGAAAT+CGG 0.448048 2.2:-65740181 MS.gene96275:CDS
CAACCGAAGGAGATGCTTAG+AGG 0.452313 2.2:+65739984 None:intergenic
AACTTCTGAAGCTGATCAAA+GGG 0.458152 2.2:-65740058 MS.gene96275:CDS
AACCACCAATTATCACCAAC+AGG 0.459595 2.2:+65740258 None:intergenic
GATGACAATGGTCGCTAACT+TGG 0.461208 2.2:-65739698 MS.gene96275:CDS
CACGTAATATCATCGCGGTT+CGG 0.462601 2.2:+65739959 None:intergenic
AAATTGGAACATTAATTGCT+GGG 0.462617 2.2:-65739738 MS.gene96275:CDS
ACTTGATTGTCTATTCATCA+AGG 0.463780 2.2:+65740113 None:intergenic
CTCTTATATATTACCTTAGG+AGG 0.464964 2.2:-65740143 MS.gene96275:CDS
TACAAGATGATCTAACCTTA+AGG 0.465621 2.2:+65739545 None:intergenic
TCGACCGAGTATCCCATATT+CGG 0.470589 2.2:+65739856 None:intergenic
GATCAGCTTCAGAAGTTGTA+GGG 0.473303 2.2:+65740064 None:intergenic
TTTCCATTACTTTCTAGAAG+TGG 0.477836 2.2:-65740750 MS.gene96275:CDS
GATCTTCTCATTCCGAATAT+GGG 0.495062 2.2:-65739868 MS.gene96275:CDS
ATGGTCGCTAACTTGGTAGA+TGG 0.495307 2.2:-65739691 MS.gene96275:CDS
CAAGTGCCTGAAATTTAGAC+AGG 0.499556 2.2:+65739632 None:intergenic
GAGGAACATGAGTTGTTGAA+TGG 0.502381 2.2:-65740306 MS.gene96275:CDS
GTTCCAATTTCAGTTATACA+TGG 0.505634 2.2:+65739751 None:intergenic
AGCCTGTTGGTGATAATTGG+TGG 0.510548 2.2:-65740260 MS.gene96275:CDS
TATACCGTCGTCAAGGGGCC+TGG 0.511288 2.2:+65739588 None:intergenic
GGGTTTACTAGAAGAAATTG+TGG 0.530266 2.2:-65740037 MS.gene96275:CDS
CAACTTCTGAAGCTGATCAA+AGG 0.539281 2.2:-65740059 MS.gene96275:CDS
CAATTATCACCAACAGGCTG+TGG 0.540795 2.2:+65740264 None:intergenic
CATCCTCTAAGCATCTCCTT+CGG 0.541272 2.2:-65739987 MS.gene96275:CDS
GCAGAGTCCACAAGTGAGGA+TGG 0.543325 2.2:-65740708 MS.gene96275:CDS
TATACATGGAGAAGCAGATA+AGG 0.544758 2.2:+65739765 None:intergenic
CATTCCGAATATGGGATACT+CGG 0.549415 2.2:-65739860 MS.gene96275:CDS
GGAGAAGCAGATAAGGATGC+AGG 0.551751 2.2:+65739772 None:intergenic
ACTAGACCAGGCTTCACTTG+TGG 0.556499 2.2:-65739890 MS.gene96275:CDS
AGTCCACTTCTAGAAAGTAA+TGG 0.557324 2.2:+65740747 None:intergenic
GTGGTATATACCGTCGTCAA+GGG 0.566981 2.2:+65739582 None:intergenic
CAACTTCATGATGTTCCTGG+TGG 0.568004 2.2:-65740669 MS.gene96275:CDS
GAAGATCCACAAGTGAAGCC+TGG 0.572388 2.2:+65739884 None:intergenic
CTTGATTGTCTATTCATCAA+GGG 0.575589 2.2:+65740114 None:intergenic
GAGAATTTAGAGAAAAGGGT+AGG 0.579452 2.2:-65739919 MS.gene96275:CDS
ACTTGATCCATCCTCACTTG+TGG 0.592828 2.2:+65740701 None:intergenic
TCAAGGGAAGAAACCTCCTA+AGG 0.594510 2.2:+65740130 None:intergenic
GGTAGGAGCTCAACTAGACC+AGG 0.597267 2.2:-65739902 MS.gene96275:CDS
AAGCAGATAAGGATGCAGGC+TGG 0.598744 2.2:+65739776 None:intergenic
ATGATATAGATTGTATTCAG+AGG 0.601793 2.2:-65739825 MS.gene96275:CDS
TCGCGGTTCGGAGCAACCGA+AGG 0.609645 2.2:+65739971 None:intergenic
TACGTTGACACCGATGACAA+TGG 0.615680 2.2:-65739710 MS.gene96275:CDS
CGTGGTATATACCGTCGTCA+AGG 0.618223 2.2:+65739581 None:intergenic
CGAACAAGCCTTCATAGCCA+AGG 0.624649 2.2:+65740383 None:intergenic
AAGTTAGCGACCATTGTCAT+CGG 0.635927 2.2:+65739700 None:intergenic
TGGTATATACCGTCGTCAAG+GGG 0.643375 2.2:+65739583 None:intergenic
TATGCCAGGCCCCTTGACGA+CGG 0.651135 2.2:-65739592 MS.gene96275:CDS
AATTGCAGAGTCCACAAGTG+AGG 0.652804 2.2:-65740712 MS.gene96275:CDS
GAGTTGATTCCTAATAAGAG+AGG 0.668136 2.2:-65740015 MS.gene96275:CDS
AATTGGAACATTAATTGCTG+GGG 0.676903 2.2:-65739737 MS.gene96275:CDS
TAAGGTATACATCTATTGCG+TGG 0.691492 2.2:+65739563 None:intergenic
ACTTCTGAAGCTGATCAAAG+GGG 0.700182 2.2:-65740057 MS.gene96275:CDS
CAAAGCGCTTGAAACGTGCG+AGG 0.701306 2.2:-65740457 MS.gene96275:CDS
GAAAGTAAACCACAGCCTGT+TGG 0.711849 2.2:-65740273 MS.gene96275:CDS
ACTTGCACGTAATATCATCG+CGG 0.804434 2.2:+65739954 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATCAATCTTTTATATAA+TGG + chr2.2:65740062-65740081 None:intergenic 10.0%
! AAAAAATTACTGGCAAAGAA+AGG + chr2.2:65740752-65740771 None:intergenic 25.0%
! AAATTGGAACATTAATTGCT+GGG - chr2.2:65740531-65740550 MS.gene96275:CDS 25.0%
! ATGATATAGATTGTATTCAG+AGG - chr2.2:65740444-65740463 MS.gene96275:CDS 25.0%
! CAAAGAGAATTTAGAGAAAA+GGG - chr2.2:65740346-65740365 MS.gene96275:CDS 25.0%
! CATCTCTTATATATTACCTT+AGG - chr2.2:65740123-65740142 MS.gene96275:CDS 25.0%
!! TAAATGAAGTTTTCAGCAAT+TGG - chr2.2:65739778-65739797 MS.gene96275:CDS 25.0%
!! TCAATCTTTTATATAATGGC+AGG + chr2.2:65740058-65740077 None:intergenic 25.0%
!!! ATTTTAGCTATTGAATCGAA+AGG - chr2.2:65740077-65740096 MS.gene96275:CDS 25.0%
AATTGGAACATTAATTGCTG+GGG - chr2.2:65740532-65740551 MS.gene96275:CDS 30.0%
ACTTGATTGTCTATTCATCA+AGG + chr2.2:65740159-65740178 None:intergenic 30.0%
CAATGCATCTAGAAACAATA+TGG + chr2.2:65739863-65739882 None:intergenic 30.0%
CTCTTATATATTACCTTAGG+AGG - chr2.2:65740126-65740145 MS.gene96275:CDS 30.0%
CTTGATTGTCTATTCATCAA+GGG + chr2.2:65740158-65740177 None:intergenic 30.0%
GAAATTGGAACATTAATTGC+TGG - chr2.2:65740530-65740549 MS.gene96275:CDS 30.0%
GCAAAGAGAATTTAGAGAAA+AGG - chr2.2:65740345-65740364 MS.gene96275:CDS 30.0%
GTTCCAATTTCAGTTATACA+TGG + chr2.2:65740521-65740540 None:intergenic 30.0%
TAATGATGCGAAAAATCAAG+AGG - chr2.2:65739944-65739963 MS.gene96275:CDS 30.0%
TACAAGATGATCTAACCTTA+AGG + chr2.2:65740727-65740746 None:intergenic 30.0%
TATGGTGCAGATAATCTTAT+TGG - chr2.2:65739738-65739757 MS.gene96275:CDS 30.0%
TCTAAACCTGTCTAAATTTC+AGG - chr2.2:65740631-65740650 MS.gene96275:CDS 30.0%
TCTAAATTCTCTTTGCATGA+TGG + chr2.2:65740341-65740360 None:intergenic 30.0%
TCTCCATGTATAACTGAAAT+TGG - chr2.2:65740515-65740534 MS.gene96275:CDS 30.0%
TGAATCGAAAGGAATGAAAT+CGG - chr2.2:65740088-65740107 MS.gene96275:CDS 30.0%
! AGATTGTATTCAGAGGATTT+TGG - chr2.2:65740451-65740470 MS.gene96275:CDS 30.0%
! CTCATAACAAAGTTTTGCTA+TGG - chr2.2:65739639-65739658 MS.gene96275:CDS 30.0%
! CTTGATTTTTCGCATCATTA+CGG + chr2.2:65739944-65739963 None:intergenic 30.0%
! TGTTATGAGCTCAAATGTTT+TGG + chr2.2:65739628-65739647 None:intergenic 30.0%
! TTTCCATTACTTTCTAGAAG+TGG - chr2.2:65739519-65739538 MS.gene96275:CDS 30.0%
ACTTGCCAATTGAACAAATC+TGG + chr2.2:65739915-65739934 None:intergenic 35.0%
CGCAATAGATGTATACCTTA+AGG - chr2.2:65740709-65740728 MS.gene96275:CDS 35.0%
CTCCAAATGACTGAAAACTA+TGG - chr2.2:65739720-65739739 MS.gene96275:CDS 35.0%
GAGAATTTAGAGAAAAGGGT+AGG - chr2.2:65740350-65740369 MS.gene96275:CDS 35.0%
GATCTTCTCATTCCGAATAT+GGG - chr2.2:65740401-65740420 MS.gene96275:CDS 35.0%
GCTGGTAGATAGACATAAAA+TGG + chr2.2:65740478-65740497 None:intergenic 35.0%
GGGTTTACTAGAAGAAATTG+TGG - chr2.2:65740232-65740251 MS.gene96275:CDS 35.0%
TAAGGTATACATCTATTGCG+TGG + chr2.2:65740709-65740728 None:intergenic 35.0%
TATACATGGAGAAGCAGATA+AGG + chr2.2:65740507-65740526 None:intergenic 35.0%
! AGTCCACTTCTAGAAAGTAA+TGG + chr2.2:65739525-65739544 None:intergenic 35.0%
! CACCATAGTTTTCAGTCATT+TGG + chr2.2:65739725-65739744 None:intergenic 35.0%
! GAGTTGATTCCTAATAAGAG+AGG - chr2.2:65740254-65740273 MS.gene96275:CDS 35.0%
!! AACTTCTGAAGCTGATCAAA+GGG - chr2.2:65740211-65740230 MS.gene96275:CDS 35.0%
!! TTCTAGATGCATTGATTCCT+TGG - chr2.2:65739869-65739888 MS.gene96275:CDS 35.0%
AACCACCAATTATCACCAAC+AGG + chr2.2:65740014-65740033 None:intergenic 40.0%
AAGTTAGCGACCATTGTCAT+CGG + chr2.2:65740572-65740591 None:intergenic 40.0%
ACTTGCACGTAATATCATCG+CGG + chr2.2:65740318-65740337 None:intergenic 40.0%
CAAGTGCCTGAAATTTAGAC+AGG + chr2.2:65740640-65740659 None:intergenic 40.0%
CATTCCGAATATGGGATACT+CGG - chr2.2:65740409-65740428 MS.gene96275:CDS 40.0%
GAGGAACATGAGTTGTTGAA+TGG - chr2.2:65739963-65739982 MS.gene96275:CDS 40.0%
GATCAGCTTCAGAAGTTGTA+GGG + chr2.2:65740208-65740227 None:intergenic 40.0%
GATGTGACACCTCTCTTATT+AGG + chr2.2:65740266-65740285 None:intergenic 40.0%
GGATCTTCTCATTCCGAATA+TGG - chr2.2:65740400-65740419 MS.gene96275:CDS 40.0%
TGATCAGCTTCAGAAGTTGT+AGG + chr2.2:65740209-65740228 None:intergenic 40.0%
TTGCAACTTCATGATGTTCC+TGG - chr2.2:65739597-65739616 MS.gene96275:CDS 40.0%
TTGCAATTCCTGATTATGCC+AGG - chr2.2:65740663-65740682 MS.gene96275:CDS 40.0%
TTGTTCAATTGGCAAGTGAC+TGG - chr2.2:65739918-65739937 MS.gene96275:CDS 40.0%
! CGGATCCAGATTTGTTCAAT+TGG - chr2.2:65739907-65739926 MS.gene96275:CDS 40.0%
!! ACTTCTGAAGCTGATCAAAG+GGG - chr2.2:65740212-65740231 MS.gene96275:CDS 40.0%
!! CAACTTCTGAAGCTGATCAA+AGG - chr2.2:65740210-65740229 MS.gene96275:CDS 40.0%
!! CATTGATTCCTTGGCTATGA+AGG - chr2.2:65739878-65739897 MS.gene96275:CDS 40.0%
AATTGCAGAGTCCACAAGTG+AGG - chr2.2:65739557-65739576 MS.gene96275:CDS 45.0%
ACTTGATCCATCCTCACTTG+TGG + chr2.2:65739571-65739590 None:intergenic 45.0%
ATGGTCGCTAACTTGGTAGA+TGG - chr2.2:65740578-65740597 MS.gene96275:CDS 45.0%
CAACTTCATGATGTTCCTGG+TGG - chr2.2:65739600-65739619 MS.gene96275:CDS 45.0%
CAATTATCACCAACAGGCTG+TGG + chr2.2:65740008-65740027 None:intergenic 45.0%
CACGTAATATCATCGCGGTT+CGG + chr2.2:65740313-65740332 None:intergenic 45.0%
CATCCTCTAAGCATCTCCTT+CGG - chr2.2:65740282-65740301 MS.gene96275:CDS 45.0%
GAAAGTAAACCACAGCCTGT+TGG - chr2.2:65739996-65740015 MS.gene96275:CDS 45.0%
GATGACAATGGTCGCTAACT+TGG - chr2.2:65740571-65740590 MS.gene96275:CDS 45.0%
GTGGTATATACCGTCGTCAA+GGG + chr2.2:65740690-65740709 None:intergenic 45.0%
TACGTTGACACCGATGACAA+TGG - chr2.2:65740559-65740578 MS.gene96275:CDS 45.0%
TCAAGCGCTTTGATGGAATC+TGG + chr2.2:65739804-65739823 None:intergenic 45.0%
TCAAGGGAAGAAACCTCCTA+AGG + chr2.2:65740142-65740161 None:intergenic 45.0%
TCGACCGAGTATCCCATATT+CGG + chr2.2:65740416-65740435 None:intergenic 45.0%
TGGTATATACCGTCGTCAAG+GGG + chr2.2:65740689-65740708 None:intergenic 45.0%
! CTTGGCTATGAAGGCTTGTT+CGG - chr2.2:65739887-65739906 MS.gene96275:CDS 45.0%
!! AGCCTGTTGGTGATAATTGG+TGG - chr2.2:65740009-65740028 MS.gene96275:CDS 45.0%
!! CACAGCCTGTTGGTGATAAT+TGG - chr2.2:65740006-65740025 MS.gene96275:CDS 45.0%
!!! TCAAATGTTTTGGCTCCACC+AGG + chr2.2:65739618-65739637 None:intergenic 45.0%
AAGCAGATAAGGATGCAGGC+TGG + chr2.2:65740496-65740515 None:intergenic 50.0%
ACTAGACCAGGCTTCACTTG+TGG - chr2.2:65740379-65740398 MS.gene96275:CDS 50.0%
CAACCGAAGGAGATGCTTAG+AGG + chr2.2:65740288-65740307 None:intergenic 50.0%
CGAACAAGCCTTCATAGCCA+AGG + chr2.2:65739889-65739908 None:intergenic 50.0%
CGTGGTATATACCGTCGTCA+AGG + chr2.2:65740691-65740710 None:intergenic 50.0%
GAAGATCCACAAGTGAAGCC+TGG + chr2.2:65740388-65740407 None:intergenic 50.0%
GCACGTTTCAAGCGCTTTGA+TGG + chr2.2:65739811-65739830 None:intergenic 50.0%
GGAGAAGCAGATAAGGATGC+AGG + chr2.2:65740500-65740519 None:intergenic 50.0%
GCAGAGTCCACAAGTGAGGA+TGG - chr2.2:65739561-65739580 MS.gene96275:CDS 55.0%
GGTAGGAGCTCAACTAGACC+AGG - chr2.2:65740367-65740386 MS.gene96275:CDS 55.0%
TCAAGGGGCCTGGCATAATC+AGG + chr2.2:65740674-65740693 None:intergenic 55.0%
! CAAAGCGCTTGAAACGTGCG+AGG - chr2.2:65739812-65739831 MS.gene96275:CDS 55.0%
TATACCGTCGTCAAGGGGCC+TGG + chr2.2:65740684-65740703 None:intergenic 60.0%
TATGCCAGGCCCCTTGACGA+CGG - chr2.2:65740677-65740696 MS.gene96275:CDS 60.0%
! TCGCGGTTCGGAGCAACCGA+AGG + chr2.2:65740301-65740320 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 65739513 65740778 65739513 ID=MS.gene96275
chr2.2 mRNA 65739513 65740778 65739513 ID=MS.gene96275.t1;Parent=MS.gene96275
chr2.2 exon 65739513 65740778 65739513 ID=MS.gene96275.t1.exon1;Parent=MS.gene96275.t1
chr2.2 CDS 65739513 65740778 65739513 ID=cds.MS.gene96275.t1;Parent=MS.gene96275.t1
Gene Sequence

>MS.gene96275

ATGCAGTTTCCATTACTTTCTAGAAGTGGACTACTAAAGAAACTAATTGCAGAGTCCACAAGTGAGGATGGATCAAGTAGTGTTTTGCAACTTCATGATGTTCCTGGTGGAGCCAAAACATTTGAGCTCATAACAAAGTTTTGCTATGGTGTGAAATTAGAAATCACAGCATTGAATGTAGTTAGTCTAAGATGTGCAGCAGAGTATCTCCAAATGACTGAAAACTATGGTGCAGATAATCTTATTGGTCACACAGAATCTTTTCTAAATGAAGTTTTCAGCAATTGGCCAGATTCCATCAAAGCGCTTGAAACGTGCGAGGAAGTGCAAAACTTTGCAGAAGATCTCCATATTGTTTCTAGATGCATTGATTCCTTGGCTATGAAGGCTTGTTCGGATCCAGATTTGTTCAATTGGCAAGTGACTGGCCGTAATGATGCGAAAAATCAAGAGGAACATGAGTTGTTGAATGGAATGTCTTGTGAAAGTAAACCACAGCCTGTTGGTGATAATTGGTGGTTTTATGATGTGTCTTTGTTGAGCCTGCCATTATATAAAAGATTGATTTTAGCTATTGAATCGAAAGGAATGAAATCGGAGACTATTGCTGCATCTCTTATATATTACCTTAGGAGGTTTCTTCCCTTGATGAATAGACAATCAAGTTTCAAAGATACAAATCATGCAACAATCCCTACAACTTCTGAAGCTGATCAAAGGGGTTTACTAGAAGAAATTGTGGAGTTGATTCCTAATAAGAGAGGTGTCACATCCTCTAAGCATCTCCTTCGGTTGCTCCGAACCGCGATGATATTACGTGCAAGTCCATCATGCAAAGAGAATTTAGAGAAAAGGGTAGGAGCTCAACTAGACCAGGCTTCACTTGTGGATCTTCTCATTCCGAATATGGGATACTCGGTCGAGACGCTCTATGATATAGATTGTATTCAGAGGATTTTGGACCATTTTATGTCTATCTACCAGCCTGCATCCTTATCTGCTTCTCCATGTATAACTGAAATTGGAACATTAATTGCTGGGGTTGATACGTTGACACCGATGACAATGGTCGCTAACTTGGTAGATGGATATCTCGCAGAAGTCGCTTCAGATGCTAATCTAAACCTGTCTAAATTTCAGGCACTTGCTGTTGCAATTCCTGATTATGCCAGGCCCCTTGACGACGGTATATACCACGCAATAGATGTATACCTTAAGGTTAGATCATCTTGTATACCTTTCTTTGCCAGTAATTTTTTTATATGA
Protein sequence

>MS.gene96275.t1

MQFPLLSRSGLLKKLIAESTSEDGSSSVLQLHDVPGGAKTFELITKFCYGVKLEITALNVVSLRCAAEYLQMTENYGADNLIGHTESFLNEVFSNWPDSIKALETCEEVQNFAEDLHIVSRCIDSLAMKACSDPDLFNWQVTGRNDAKNQEEHELLNGMSCESKPQPVGDNWWFYDVSLLSLPLYKRLILAIESKGMKSETIAASLIYYLRRFLPLMNRQSSFKDTNHATIPTTSEADQRGLLEEIVELIPNKRGVTSSKHLLRLLRTAMILRASPSCKENLEKRVGAQLDQASLVDLLIPNMGYSVETLYDIDCIQRILDHFMSIYQPASLSASPCITEIGTLIAGVDTLTPMTMVANLVDGYLAEVASDANLNLSKFQALAVAIPDYARPLDDGIYHAIDVYLKVRSSCIPFFASNFFI