Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98071.t1 | AES97097.1 | 97.7 | 261 | 6 | 0 | 1 | 261 | 1 | 261 | 6.30E-144 | 520 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98071.t1 | G7JZ18 | 97.7 | 261 | 6 | 0 | 1 | 261 | 1 | 261 | 4.6e-144 | 520.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049993 | MS.gene98071 | 0.800784 | 1.23E-48 | -1.69E-46 |
| MS.gene051860 | MS.gene98071 | 0.814798 | 1.28E-51 | -1.69E-46 |
| MS.gene05288 | MS.gene98071 | 0.801104 | 1.05E-48 | -1.69E-46 |
| MS.gene054184 | MS.gene98071 | 0.803434 | 3.49E-49 | -1.69E-46 |
| MS.gene054713 | MS.gene98071 | 0.924513 | 6.37E-90 | -1.69E-46 |
| MS.gene055860 | MS.gene98071 | 0.826656 | 2.41E-54 | -1.69E-46 |
| MS.gene058042 | MS.gene98071 | 0.907884 | 3.13E-81 | -1.69E-46 |
| MS.gene061114 | MS.gene98071 | -0.808974 | 2.38E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98071.t1 | MTR_5g045300 | 97.701 | 261 | 6 | 0 | 1 | 261 | 1 | 261 | 0.0 | 519 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTATTTCCTTATTCTTTAA+TGG | 0.128917 | 5.4:-36004177 | MS.gene98071:CDS |
| GGTTTCTTCAACCTTGTTAT+TGG | 0.181137 | 5.4:-36004393 | MS.gene98071:CDS |
| AGTACCCTCTTTAGTTTATT+GGG | 0.199150 | 5.4:-36004487 | MS.gene98071:CDS |
| TACATTTCCATTAAAGAATA+AGG | 0.220724 | 5.4:+36004170 | None:intergenic |
| AGCTATATCTTGGGTTAGTT+TGG | 0.236897 | 5.4:-36004094 | MS.gene98071:CDS |
| TTCTATGTTTGTGTGGATTA+AGG | 0.264606 | 5.4:-36004067 | MS.gene98071:CDS |
| GAGTACCCTCTTTAGTTTAT+TGG | 0.311311 | 5.4:-36004488 | MS.gene98071:CDS |
| CTTGAGTGTCATGCTCATTT+CGG | 0.326197 | 5.4:-36004528 | MS.gene98071:CDS |
| TTCGGAATCTTTGTTGGGAT+TGG | 0.345094 | 5.4:-36004510 | MS.gene98071:CDS |
| GTTTCCCAATACCAATCTAA+TGG | 0.354508 | 5.4:+36004450 | None:intergenic |
| CTCATTTCGGAATCTTTGTT+GGG | 0.357232 | 5.4:-36004515 | MS.gene98071:CDS |
| GCAGCCCATTAGATTGGTAT+TGG | 0.375445 | 5.4:-36004455 | MS.gene98071:CDS |
| GCTCATTTCGGAATCTTTGT+TGG | 0.394737 | 5.4:-36004516 | MS.gene98071:CDS |
| ATGTTGGTAAAGCTATATCT+TGG | 0.394828 | 5.4:-36004104 | MS.gene98071:CDS |
| GCTATATCTTGGGTTAGTTT+GGG | 0.395217 | 5.4:-36004093 | MS.gene98071:CDS |
| AAGTAAAGGAGGTTCATAAA+AGG | 0.403972 | 5.4:+36004245 | None:intergenic |
| CAGCCCATTAGATTGGTATT+GGG | 0.406163 | 5.4:-36004454 | MS.gene98071:CDS |
| AATTTGAAGTATAAACTTAG+TGG | 0.421521 | 5.4:+36004555 | None:intergenic |
| TGTAACTAGTTCTTGATCCT+TGG | 0.433441 | 5.4:+36003945 | None:intergenic |
| TTTATGAACCTCCTTTACTT+AGG | 0.461805 | 5.4:-36004242 | MS.gene98071:CDS |
| TAAGATTAAACTCAAAGGGT+TGG | 0.466105 | 5.4:-36004310 | MS.gene98071:CDS |
| ATCCTTGCTTTCATGAATGA+AGG | 0.475263 | 5.4:-36004636 | MS.gene98071:CDS |
| TTTCCCAATACCAATCTAAT+GGG | 0.484056 | 5.4:+36004451 | None:intergenic |
| ATCGTAAGATTAAACTCAAA+GGG | 0.489443 | 5.4:-36004314 | MS.gene98071:CDS |
| ACTATGTCTTGAGAAGAAAC+TGG | 0.491172 | 5.4:+36004219 | None:intergenic |
| CAACTCGCAGCCCATTAGAT+TGG | 0.497410 | 5.4:-36004461 | MS.gene98071:CDS |
| TGCTCAATCGTTTAATCGAG+TGG | 0.498193 | 5.4:-36004344 | MS.gene98071:CDS |
| TGTTGGTAAAGCTATATCTT+GGG | 0.500531 | 5.4:-36004103 | MS.gene98071:CDS |
| CGTCAATGAACAATGGTCAT+GGG | 0.519308 | 5.4:-36004589 | MS.gene98071:CDS |
| TTGTGTGCCTTCTCTTCAAG+TGG | 0.521153 | 5.4:+36004012 | None:intergenic |
| GAAGAAACTGGCCTAAGTAA+AGG | 0.522889 | 5.4:+36004231 | None:intergenic |
| TCGTCAATGAACAATGGTCA+TGG | 0.527571 | 5.4:-36004590 | MS.gene98071:CDS |
| GTACCCTCTTTAGTTTATTG+GGG | 0.529611 | 5.4:-36004486 | MS.gene98071:CDS |
| TTCTCTTCAAGTGGTATCTG+TGG | 0.535124 | 5.4:+36004021 | None:intergenic |
| TGCACCCCAATAAACTAAAG+AGG | 0.538582 | 5.4:+36004482 | None:intergenic |
| CAGATTTACACACATGATGT+TGG | 0.543563 | 5.4:-36004120 | MS.gene98071:CDS |
| GATCGTAAGATTAAACTCAA+AGG | 0.575574 | 5.4:-36004315 | MS.gene98071:CDS |
| ACAGATACCACTTGAAGAGA+AGG | 0.584939 | 5.4:-36004019 | MS.gene98071:CDS |
| TGGGAAGTTCTATGTTTGTG+TGG | 0.587350 | 5.4:-36004074 | MS.gene98071:CDS |
| AGATGACAAGAAAGAGTCCA+AGG | 0.588905 | 5.4:-36003962 | MS.gene98071:CDS |
| GACGAAGCTATTAGAGAGTG+AGG | 0.597104 | 5.4:+36004608 | None:intergenic |
| AGCCTTCATTCATGAAAGCA+AGG | 0.601961 | 5.4:+36004634 | None:intergenic |
| GAAACTGGCCTAAGTAAAGG+AGG | 0.623329 | 5.4:+36004234 | None:intergenic |
| GCACCCCAATAAACTAAAGA+GGG | 0.630654 | 5.4:+36004483 | None:intergenic |
| AAGCAAGGATACATATTGAG+AGG | 0.635812 | 5.4:+36004649 | None:intergenic |
| GTCAATGAACAATGGTCATG+GGG | 0.649937 | 5.4:-36004588 | MS.gene98071:CDS |
| GAAGCTATTAGAGAGTGAGG+AGG | 0.662308 | 5.4:+36004611 | None:intergenic |
| ATAGCTTCGTCAATGAACAA+TGG | 0.663464 | 5.4:-36004596 | MS.gene98071:CDS |
| AATGTAACAATCCAATAACA+AGG | 0.690049 | 5.4:+36004382 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTATTTCCTTATTCTTTAA+TGG | - | chr5.4:36004440-36004459 | MS.gene98071:CDS | 20.0% |
| !! | TACATTTCCATTAAAGAATA+AGG | + | chr5.4:36004450-36004469 | None:intergenic | 20.0% |
| !!! | AATGTTGAAATTTTGAAAAC+TGG | - | chr5.4:36004203-36004222 | MS.gene98071:CDS | 20.0% |
| !!! | AATTTGAAGTATAAACTTAG+TGG | + | chr5.4:36004065-36004084 | None:intergenic | 20.0% |
| ! | AATGTAACAATCCAATAACA+AGG | + | chr5.4:36004238-36004257 | None:intergenic | 25.0% |
| ! | ATCGTAAGATTAAACTCAAA+GGG | - | chr5.4:36004303-36004322 | MS.gene98071:CDS | 25.0% |
| !! | TGAATGAACTTTTCAATATC+AGG | + | chr5.4:36003945-36003964 | None:intergenic | 25.0% |
| !!! | GAAATGTATTGCTTTTAAGT+TGG | - | chr5.4:36004462-36004481 | MS.gene98071:CDS | 25.0% |
| AAGTAAAGGAGGTTCATAAA+AGG | + | chr5.4:36004375-36004394 | None:intergenic | 30.0% | |
| GATCGTAAGATTAAACTCAA+AGG | - | chr5.4:36004302-36004321 | MS.gene98071:CDS | 30.0% | |
| TAAGATTAAACTCAAAGGGT+TGG | - | chr5.4:36004307-36004326 | MS.gene98071:CDS | 30.0% | |
| TTTATGAACCTCCTTTACTT+AGG | - | chr5.4:36004375-36004394 | MS.gene98071:CDS | 30.0% | |
| TTTCCCAATACCAATCTAAT+GGG | + | chr5.4:36004169-36004188 | None:intergenic | 30.0% | |
| ! | AGTACCCTCTTTAGTTTATT+GGG | - | chr5.4:36004130-36004149 | MS.gene98071:CDS | 30.0% |
| ! | ATGTTGGTAAAGCTATATCT+TGG | - | chr5.4:36004513-36004532 | MS.gene98071:CDS | 30.0% |
| ! | CCAACTCTGAATTTTTCTTA+GGG | + | chr5.4:36004343-36004362 | None:intergenic | 30.0% |
| ! | TCCAACTCTGAATTTTTCTT+AGG | + | chr5.4:36004344-36004363 | None:intergenic | 30.0% |
| ! | TGTTGGTAAAGCTATATCTT+GGG | - | chr5.4:36004514-36004533 | MS.gene98071:CDS | 30.0% |
| ! | TTCTATGTTTGTGTGGATTA+AGG | - | chr5.4:36004550-36004569 | MS.gene98071:CDS | 30.0% |
| !!! | CTTTTAAGTTGGTGTTTTTC+TGG | - | chr5.4:36004473-36004492 | MS.gene98071:CDS | 30.0% |
| AAGCAAGGATACATATTGAG+AGG | + | chr5.4:36003971-36003990 | None:intergenic | 35.0% | |
| ACTATGTCTTGAGAAGAAAC+TGG | + | chr5.4:36004401-36004420 | None:intergenic | 35.0% | |
| ATAGCTTCGTCAATGAACAA+TGG | - | chr5.4:36004021-36004040 | MS.gene98071:CDS | 35.0% | |
| ATCCTTGCTTTCATGAATGA+AGG | - | chr5.4:36003981-36004000 | MS.gene98071:CDS | 35.0% | |
| CAGATTTACACACATGATGT+TGG | - | chr5.4:36004497-36004516 | MS.gene98071:CDS | 35.0% | |
| CCCTAAGAAAAATTCAGAGT+TGG | - | chr5.4:36004340-36004359 | MS.gene98071:CDS | 35.0% | |
| CTCATTTCGGAATCTTTGTT+GGG | - | chr5.4:36004102-36004121 | MS.gene98071:CDS | 35.0% | |
| GTTTCCCAATACCAATCTAA+TGG | + | chr5.4:36004170-36004189 | None:intergenic | 35.0% | |
| ! | GAGTACCCTCTTTAGTTTAT+TGG | - | chr5.4:36004129-36004148 | MS.gene98071:CDS | 35.0% |
| ! | GGTTTCTTCAACCTTGTTAT+TGG | - | chr5.4:36004224-36004243 | MS.gene98071:CDS | 35.0% |
| ! | GTACCCTCTTTAGTTTATTG+GGG | - | chr5.4:36004131-36004150 | MS.gene98071:CDS | 35.0% |
| ! | TGTAACTAGTTCTTGATCCT+TGG | + | chr5.4:36004675-36004694 | None:intergenic | 35.0% |
| !! | AGCTATATCTTGGGTTAGTT+TGG | - | chr5.4:36004523-36004542 | MS.gene98071:CDS | 35.0% |
| !! | GCTATATCTTGGGTTAGTTT+GGG | - | chr5.4:36004524-36004543 | MS.gene98071:CDS | 35.0% |
| ACAGATACCACTTGAAGAGA+AGG | - | chr5.4:36004598-36004617 | MS.gene98071:CDS | 40.0% | |
| AGATGACAAGAAAGAGTCCA+AGG | - | chr5.4:36004655-36004674 | MS.gene98071:CDS | 40.0% | |
| AGCCTTCATTCATGAAAGCA+AGG | + | chr5.4:36003986-36004005 | None:intergenic | 40.0% | |
| CAGCCCATTAGATTGGTATT+GGG | - | chr5.4:36004163-36004182 | MS.gene98071:CDS | 40.0% | |
| CGTCAATGAACAATGGTCAT+GGG | - | chr5.4:36004028-36004047 | MS.gene98071:CDS | 40.0% | |
| CTTGAGTGTCATGCTCATTT+CGG | - | chr5.4:36004089-36004108 | MS.gene98071:CDS | 40.0% | |
| GCACCCCAATAAACTAAAGA+GGG | + | chr5.4:36004137-36004156 | None:intergenic | 40.0% | |
| GCTCATTTCGGAATCTTTGT+TGG | - | chr5.4:36004101-36004120 | MS.gene98071:CDS | 40.0% | |
| GTCAATGAACAATGGTCATG+GGG | - | chr5.4:36004029-36004048 | MS.gene98071:CDS | 40.0% | |
| TCGTCAATGAACAATGGTCA+TGG | - | chr5.4:36004027-36004046 | MS.gene98071:CDS | 40.0% | |
| TGCACCCCAATAAACTAAAG+AGG | + | chr5.4:36004138-36004157 | None:intergenic | 40.0% | |
| TGCTCAATCGTTTAATCGAG+TGG | - | chr5.4:36004273-36004292 | MS.gene98071:CDS | 40.0% | |
| TGGGAAGTTCTATGTTTGTG+TGG | - | chr5.4:36004543-36004562 | MS.gene98071:CDS | 40.0% | |
| TTCGGAATCTTTGTTGGGAT+TGG | - | chr5.4:36004107-36004126 | MS.gene98071:CDS | 40.0% | |
| TTCTCTTCAAGTGGTATCTG+TGG | + | chr5.4:36004599-36004618 | None:intergenic | 40.0% | |
| ! | GAAGAAACTGGCCTAAGTAA+AGG | + | chr5.4:36004389-36004408 | None:intergenic | 40.0% |
| GAAGCTATTAGAGAGTGAGG+AGG | + | chr5.4:36004009-36004028 | None:intergenic | 45.0% | |
| GACGAAGCTATTAGAGAGTG+AGG | + | chr5.4:36004012-36004031 | None:intergenic | 45.0% | |
| GCAGCCCATTAGATTGGTAT+TGG | - | chr5.4:36004162-36004181 | MS.gene98071:CDS | 45.0% | |
| TTGTGTGCCTTCTCTTCAAG+TGG | + | chr5.4:36004608-36004627 | None:intergenic | 45.0% | |
| ! | GAAACTGGCCTAAGTAAAGG+AGG | + | chr5.4:36004386-36004405 | None:intergenic | 45.0% |
| CAACTCGCAGCCCATTAGAT+TGG | - | chr5.4:36004156-36004175 | MS.gene98071:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 36003927 | 36004712 | 36003927 | ID=MS.gene98071 |
| chr5.4 | mRNA | 36003927 | 36004712 | 36003927 | ID=MS.gene98071.t1;Parent=MS.gene98071 |
| chr5.4 | exon | 36003927 | 36004712 | 36003927 | ID=MS.gene98071.t1.exon1;Parent=MS.gene98071.t1 |
| chr5.4 | CDS | 36003927 | 36004712 | 36003927 | ID=cds.MS.gene98071.t1;Parent=MS.gene98071.t1 |
| Gene Sequence |
| Protein sequence |
