Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98787.t1 | KEH23151.1 | 98.3 | 115 | 2 | 0 | 1 | 115 | 1 | 115 | 2.30E-50 | 208 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98787.t1 | A0A072U114 | 98.3 | 115 | 2 | 0 | 1 | 115 | 1 | 115 | 1.7e-50 | 208.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene98787 | 0.834443 | 2.99E-56 | -1.69E-46 |
| MS.gene050563 | MS.gene98787 | 0.832402 | 9.65E-56 | -1.69E-46 |
| MS.gene051720 | MS.gene98787 | 0.801024 | 1.09E-48 | -1.69E-46 |
| MS.gene053383 | MS.gene98787 | 0.828062 | 1.11E-54 | -1.69E-46 |
| MS.gene05456 | MS.gene98787 | 0.80382 | 2.90E-49 | -1.69E-46 |
| MS.gene054791 | MS.gene98787 | 0.816616 | 5.04E-52 | -1.69E-46 |
| MS.gene055359 | MS.gene98787 | 0.812251 | 4.66E-51 | -1.69E-46 |
| MS.gene058918 | MS.gene98787 | 0.804419 | 2.18E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98787.t1 | MTR_7g067365 | 98.261 | 115 | 2 | 0 | 1 | 115 | 1 | 115 | 1.77e-75 | 219 |
| MS.gene98787.t1 | MTR_2g013470 | 54.098 | 122 | 43 | 3 | 4 | 114 | 4 | 123 | 2.73e-23 | 87.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGACAATCTTGATTGCTCTT+TGG | 0.335761 | 7.4:+36014655 | None:intergenic |
| TCTTACATTCTTCTTCATCT+AGG | 0.348003 | 7.4:-36014454 | MS.gene98787:CDS |
| GTTGGTATGTCATTGACATT+AGG | 0.356920 | 7.4:+36014725 | None:intergenic |
| GGTTTCTTCTTCATCTACTT+TGG | 0.392407 | 7.4:-36014546 | MS.gene98787:CDS |
| AAATGTCCATTAAAAGCAAA+AGG | 0.418577 | 7.4:-36014760 | MS.gene98787:CDS |
| CTTACATTCTTCTTCATCTA+GGG | 0.436392 | 7.4:-36014453 | MS.gene98787:CDS |
| CTTCTTCATCTACTTTGGTA+AGG | 0.453056 | 7.4:-36014541 | MS.gene98787:CDS |
| TTGAAATTCCTAGTGCAACT+AGG | 0.484774 | 7.4:-36014626 | MS.gene98787:CDS |
| AACTCAACTCAACTGAAACT+TGG | 0.493461 | 7.4:+36014425 | None:intergenic |
| AGTTTCAGCTCGATGTGAAT+CGG | 0.520615 | 7.4:+36014496 | None:intergenic |
| AATGTCCATTAAAAGCAAAA+GGG | 0.546646 | 7.4:-36014759 | MS.gene98787:CDS |
| CTATGAAAAGAAGATCCTCT+AGG | 0.575974 | 7.4:-36014571 | MS.gene98787:CDS |
| TACTTTGGTAAGGTCGCGAT+CGG | 0.577817 | 7.4:-36014531 | MS.gene98787:CDS |
| AGTTGCACTAGGAATTTCAA+AGG | 0.597401 | 7.4:+36014629 | None:intergenic |
| AAGAAGAGGGAAATTGAAAG+TGG | 0.598757 | 7.4:-36014692 | MS.gene98787:CDS |
| GAAAAGAAGATCCTCTAGGA+AGG | 0.606833 | 7.4:-36014567 | MS.gene98787:CDS |
| AGTGCAACTAGGAACAAAAG+TGG | 0.620566 | 7.4:-36014615 | MS.gene98787:CDS |
| CAGCTCGATGTGAATCGGTG+AGG | 0.671793 | 7.4:+36014501 | None:intergenic |
| TGAAGAAGAAACCTTCCTAG+AGG | 0.674397 | 7.4:+36014556 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACATTTTTTCTCAAGAAGA+GGG | - | chr7.4:36014486-36014505 | MS.gene98787:CDS | 25.0% |
| CTCTTCTTGAGAAAAAATGT+TGG | + | chr7.4:36014487-36014506 | None:intergenic | 30.0% | |
| CTTACATTCTTCTTCATCTA+GGG | - | chr7.4:36014738-36014757 | MS.gene98787:CDS | 30.0% | |
| TCTTACATTCTTCTTCATCT+AGG | - | chr7.4:36014737-36014756 | MS.gene98787:CDS | 30.0% | |
| !!! | CAACATTTTTTCTCAAGAAG+AGG | - | chr7.4:36014485-36014504 | MS.gene98787:CDS | 30.0% |
| AAGAAGAGGGAAATTGAAAG+TGG | - | chr7.4:36014499-36014518 | MS.gene98787:CDS | 35.0% | |
| AGTTGCACTAGGAATTTCAA+AGG | + | chr7.4:36014565-36014584 | None:intergenic | 35.0% | |
| CTATGAAAAGAAGATCCTCT+AGG | - | chr7.4:36014620-36014639 | MS.gene98787:CDS | 35.0% | |
| CTTCTTCATCTACTTTGGTA+AGG | - | chr7.4:36014650-36014669 | MS.gene98787:CDS | 35.0% | |
| GGTTTCTTCTTCATCTACTT+TGG | - | chr7.4:36014645-36014664 | MS.gene98787:CDS | 35.0% | |
| GTTGGTATGTCATTGACATT+AGG | + | chr7.4:36014469-36014488 | None:intergenic | 35.0% | |
| TGACAATCTTGATTGCTCTT+TGG | + | chr7.4:36014539-36014558 | None:intergenic | 35.0% | |
| TTGAAATTCCTAGTGCAACT+AGG | - | chr7.4:36014565-36014584 | MS.gene98787:CDS | 35.0% | |
| !! | GCATACCCTTTTGCTTTTAA+TGG | + | chr7.4:36014440-36014459 | None:intergenic | 35.0% |
| AGTGCAACTAGGAACAAAAG+TGG | - | chr7.4:36014576-36014595 | MS.gene98787:CDS | 40.0% | |
| AGTTTCAGCTCGATGTGAAT+CGG | + | chr7.4:36014698-36014717 | None:intergenic | 40.0% | |
| TGAAGAAGAAACCTTCCTAG+AGG | + | chr7.4:36014638-36014657 | None:intergenic | 40.0% | |
| ! | CTTTTGTTCCTAGTTGCACT+AGG | + | chr7.4:36014576-36014595 | None:intergenic | 40.0% |
| ! | GAAAAGAAGATCCTCTAGGA+AGG | - | chr7.4:36014624-36014643 | MS.gene98787:CDS | 40.0% |
| TACTTTGGTAAGGTCGCGAT+CGG | - | chr7.4:36014660-36014679 | MS.gene98787:CDS | 45.0% | |
| CAGCTCGATGTGAATCGGTG+AGG | + | chr7.4:36014693-36014712 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 36014433 | 36014780 | 36014433 | ID=MS.gene98787 |
| chr7.4 | mRNA | 36014433 | 36014780 | 36014433 | ID=MS.gene98787.t1;Parent=MS.gene98787 |
| chr7.4 | exon | 36014433 | 36014780 | 36014433 | ID=MS.gene98787.t1.exon1;Parent=MS.gene98787.t1 |
| chr7.4 | CDS | 36014433 | 36014780 | 36014433 | ID=cds.MS.gene98787.t1;Parent=MS.gene98787.t1 |
| Gene Sequence |
| Protein sequence |
