AlfalfaGEDB | MS.gene99142

M. sativa cultivar XinJiangDaYe / MS.gene99142

KEGG: -
GO: GO:0003674 GO:0003824 GO:0009055 GO:0016491
PFAM: PF02298.20

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99142.t1	AFK43360.1	93.5	199	9	1	1	195	1	199	8.00E-67	263.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99142.t1	Q96316	49.2	124	61	1	1	124	1	122	2.8e-28	126.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99142.t1	I3SSW8	93.5	199	9	1	1	195	1	199	5.8e-67	263.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050363	MS.gene99142	0.833716	4.54E-56	-1.69E-46
MS.gene051946	MS.gene99142	0.801082	1.07E-48	-1.69E-46
MS.gene055401	MS.gene99142	0.99852	1.54E-267	-1.69E-46
MS.gene056310	MS.gene99142	0.85236	4.86E-61	-1.69E-46
MS.gene056311	MS.gene99142	0.816034	6.81E-52	-1.69E-46
MS.gene056313	MS.gene99142	0.836738	7.85E-57	-1.69E-46
MS.gene056408	MS.gene99142	0.80038	1.48E-48	-1.69E-46
MS.gene059517	MS.gene99142	-0.803098	4.10E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99142.t1	MTR_8g007020	88.718	195	14	1	1	195	1	187	5.67e-111	315
MS.gene99142.t1	MTR_8g007035	87.940	199	20	1	1	195	1	199	9.34e-105	300
MS.gene99142.t1	MTR_3g099580	56.303	119	49	2	2	120	5	120	1.25e-36	126
MS.gene99142.t1	MTR_3g099570	48.201	139	65	3	2	136	5	140	2.68e-34	120
MS.gene99142.t1	MTR_3g099540	49.231	130	62	3	2	130	5	131	2.20e-33	116
MS.gene99142.t1	MTR_3g099680	51.261	119	54	4	4	120	6	122	9.79e-31	112
MS.gene99142.t1	MTR_2g088990	39.726	146	83	3	8	152	11	152	1.18e-29	109
MS.gene99142.t1	MTR_5g006040	36.774	155	87	4	9	160	11	157	2.12e-29	107
MS.gene99142.t1	MTR_1g104800	34.211	152	95	3	11	159	12	161	5.14e-28	104
MS.gene99142.t1	MTR_3g099980	46.377	138	58	5	4	127	6	141	1.53e-26	101

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99142.t1	AT2G44790	53.704	108	46	1	17	124	25	128	6.56e-35	122
MS.gene99142.t1	AT3G60270	52.336	107	49	1	17	123	19	123	2.77e-34	120
MS.gene99142.t1	AT3G60280	53.271	107	48	1	17	123	17	121	8.26e-34	120
MS.gene99142.t1	AT2G32300	50.847	118	51	3	21	133	23	138	6.44e-33	119
MS.gene99142.t1	AT2G32300	45.270	148	68	4	21	167	53	188	1.43e-32	119
MS.gene99142.t1	AT1G72230	45.513	156	77	4	2	153	3	154	1.44e-31	113
MS.gene99142.t1	AT3G27200	41.803	122	68	2	1	121	3	122	7.72e-28	103
MS.gene99142.t1	AT5G07475	38.514	148	84	2	3	145	4	149	2.94e-27	102
MS.gene99142.t1	AT2G31050	42.336	137	70	4	8	137	8	142	2.15e-26	100
MS.gene99142.t1	AT2G26720	39.706	136	73	4	9	137	9	142	2.75e-25	97.8

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Find 43 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAGTTGCAAATGCATA+AGG	0.473584	8.2:+82740803	None:intergenic
AACTCCTTGATTCCATCCAC+TGG	0.514824	8.2:+82740766	None:intergenic
AACTTCATCCACTTGGTGGA+AGG	0.561488	8.2:+82740445	None:intergenic
AACTTCTCCATCACCACCAA+AGG	0.566009	8.2:-82740144	MS.gene99142:CDS
AAGGAGTTGATTACACCAAA+TGG	0.428315	8.2:-82740751	MS.gene99142:CDS
AAGGTTGCATTGACTAAAGC+AGG	0.472415	8.2:-82740356	MS.gene99142:CDS
ACAGCTCCATTATCCTTTGG+TGG	0.526576	8.2:+82740131	None:intergenic
AGCACCACCCCAACTCCAAG+TGG	0.615986	8.2:-82740251	MS.gene99142:CDS
AGGAGTTGATTACACCAAAT+GGG	0.504844	8.2:-82740750	MS.gene99142:CDS
AGTTGCAAATGCATAAGGAA+AGG	0.617334	8.2:+82740808	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCAGTTGCAAATGCATA+AGG	+	chr8.2:82740106-82740125	None:intergenic	30.0%
TAGGTATGTATACATTCTGA+CGG	-	chr8.2:82740212-82740231	MS.gene99142:CDS	30.0%
TCTGGAAAAACTTTCAAAGT+TGG	-	chr8.2:82740181-82740200	MS.gene99142:CDS	30.0%
AGTTGCAAATGCATAAGGAA+AGG	+	chr8.2:82740101-82740120	None:intergenic	35.0%
CATCAAAAGACTAACACCAT+TGG	+	chr8.2:82740809-82740828	None:intergenic	35.0%
CTATGCTGATGGAAATTCTA+AGG	-	chr8.2:82740531-82740550	MS.gene99142:intron	35.0%
TCCATCAGCATAGTTCTTTA+TGG	+	chr8.2:82740524-82740543	None:intergenic	35.0%
TTTCATCAACTTCATCCACT+TGG	+	chr8.2:82740471-82740490	None:intergenic	35.0%
CTAACAGCTCCATTATCCTT+TGG	+	chr8.2:82740781-82740800	None:intergenic	40.0%
GAAGTTGAGTTTGTCTCTGA+AGG	+	chr8.2:82740748-82740767	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	82740055	82740873	82740055	ID=MS.gene99142
chr8.2	mRNA	82740055	82740873	82740055	ID=MS.gene99142.t1;Parent=MS.gene99142
chr8.2	exon	82740714	82740873	82740714	ID=MS.gene99142.t1.exon1;Parent=MS.gene99142.t1
chr8.2	CDS	82740714	82740873	82740714	ID=cds.MS.gene99142.t1;Parent=MS.gene99142.t1
chr8.2	exon	82740055	82740482	82740055	ID=MS.gene99142.t1.exon2;Parent=MS.gene99142.t1
chr8.2	CDS	82740055	82740482	82740055	ID=cds.MS.gene99142.t1;Parent=MS.gene99142.t1

Gene Sequence

>MS.gene99142

ATGAATACATCAATGATAGCTTCTTTCTTTGTTCTATTGTTGGCCTTTCCTTATGCATTTGCAACTGATTTCACTGTTGGTGATGCCAGTGGATGGAATCAAGGAGTTGATTACACCAAATGGGCTTCTGGAAAAACTTTCAAAGTTGGTGATAACTTAGGTATGTATACATTCTGACGGCGCACAGCTTGTTGCTTGCTTGCCTTTCGTTTATATACTGAAATATGAATATTATGTTTTTTCTTATTTGGAAAAAATGAAAAAGTTTGATAATGCTAAATTTGAGGTCTAAAAATACTATTACATCATGAAATCTTAAATATAGAAATAATGTAAAAGTATTTTGCAATCTACATTAGAGTTAACAATTTTTTGTGTGTTGAAAAAACAGTGTTCAAATATGGCTCCTTCCACCAAGTGGATGAAGTTGATGAAAGTGACTACAAAAGCTGCACTTCAAGCAACGCCATAAAGAACTATGCTGATGGAAATTCTAAGGTTGCATTGACTAAAGCAGGAAAAATTTACTTCATATGCCCTACTCCAGGTCATTGTACTAGTACTGGTGGCATGAAGCTTGAAGTCAATGTTGTTGCTGCTAGCACCACCCCAACTCCAAGTGGCACCCCCCCACCAACAAAATCTCCATCAACCACACCATCAACTACTCCATCAACCACACCATCAGCTCCTTCAGAGACAAACTCAACTTCTCCATCACCACCAAAGGATAATGGAGCTGTTAGTGTTTCCAATGGTGTTAGTCTTTTGATGGGTTCTTTCTTTGTTTCAGCAATGATTTTGGGTTTGATGGGCTAG

Protein sequence

>MS.gene99142.t1

MNTSMIASFFVLLLAFPYAFATDFTVGDASGWNQGVDYTKWASGKTFKVGDNLVFKYGSFHQVDEVDESDYKSCTSSNAIKNYADGNSKVALTKAGKIYFICPTPGHCTSTGGMKLEVNVVAASTTPTPSGTPPPTKSPSTTPSTTPSTTPSAPSETNSTSPSPPKDNGAVSVSNGVSLLMGSFFVSAMILGLMG