Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049046.01.T01 | KEH41194.1 | 97.447 | 235 | 6 | 0 | 1 | 235 | 2 | 236 | 2.14E-162 | 458 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049046.01.T01 | Q9T0H8 | 68.803 | 234 | 66 | 2 | 2 | 235 | 18 | 244 | 5.64E-108 | 313 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049046.01.T01 | A0A072VGM0 | 97.447 | 235 | 6 | 0 | 1 | 235 | 2 | 236 | 1.02e-162 | 458 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048134.01 | MsG0080049046.01 | 0.810858 | 9.351553e-51 | 5.700812e-48 |
| MsG0080049046.01 | MsG0180001915.01 | 0.991761 | 2.130073e-189 | 1.723908e-182 |
| MsG0080049046.01 | MsG0180003320.01 | 0.805595 | 1.237353e-49 | 6.572623e-47 |
| MsG0080049046.01 | MsG0180003849.01 | 0.815601 | 8.502818e-52 | 5.888881e-49 |
| MsG0080049046.01 | MsG0180005572.01 | 0.807017 | 6.205559e-50 | 3.418785e-47 |
| MsG0080049046.01 | MsG0280007678.01 | 0.806532 | 7.857329e-50 | 4.275047e-47 |
| MsG0080049046.01 | MsG0280009232.01 | 0.824266 | 8.860110e-54 | 7.808978e-51 |
| MsG0080049046.01 | MsG0380014344.01 | 0.806042 | 9.968651e-50 | 5.356352e-47 |
| MsG0080049046.01 | MsG0380016299.01 | 0.835880 | 1.296533e-56 | 1.606655e-53 |
| MsG0080049046.01 | MsG0380016300.01 | 0.831079 | 2.045947e-55 | 2.196707e-52 |
| MsG0080049046.01 | MsG0380017030.01 | 0.808123 | 3.614409e-50 | 2.049604e-47 |
| MsG0080049046.01 | MsG0380017168.01 | 0.801801 | 7.585056e-49 | 3.655770e-46 |
| MsG0080049046.01 | MsG0380017901.01 | 0.807082 | 6.011954e-50 | 3.317633e-47 |
| MsG0080049046.01 | MsG0480018433.01 | 0.817220 | 3.691861e-52 | 2.672589e-49 |
| MsG0080049046.01 | MsG0480019146.01 | 0.829287 | 5.601906e-55 | 5.709186e-52 |
| MsG0080049046.01 | MsG0480020904.01 | 0.848754 | 5.010161e-60 | 9.289433e-57 |
| MsG0080049046.01 | MsG0480021360.01 | 0.832400 | 9.662532e-56 | 1.078796e-52 |
| MsG0080049046.01 | MsG0480022055.01 | 0.805530 | 1.276573e-49 | 6.769613e-47 |
| MsG0080049046.01 | MsG0580024087.01 | 0.831287 | 1.818618e-55 | 1.964574e-52 |
| MsG0080049046.01 | MsG0580025590.01 | 0.825517 | 4.490731e-54 | 4.102365e-51 |
| MsG0080049046.01 | MsG0580026521.01 | 0.820829 | 5.576376e-53 | 4.460631e-50 |
| MsG0080049046.01 | MsG0580029674.01 | 0.803091 | 4.113662e-49 | 2.048735e-46 |
| MsG0080049046.01 | MsG0580029676.01 | 0.800674 | 1.289911e-48 | 6.042332e-46 |
| MsG0080049046.01 | MsG0680030296.01 | 0.803151 | 3.997650e-49 | 1.994176e-46 |
| MsG0080049046.01 | MsG0680030613.01 | 0.811904 | 5.543690e-51 | 3.474050e-48 |
| MsG0080049046.01 | MsG0680034238.01 | 0.806269 | 8.926796e-50 | 4.824693e-47 |
| MsG0080049046.01 | MsG0680034418.01 | 0.812868 | 3.413171e-51 | 2.194737e-48 |
| MsG0080049046.01 | MsG0780036839.01 | 0.836361 | 9.788363e-57 | 1.230785e-53 |
| MsG0080049046.01 | MsG0780038519.01 | 0.802986 | 4.324056e-49 | 2.147831e-46 |
| MsG0080049046.01 | MsG0780039619.01 | 0.827708 | 1.347554e-54 | 1.311160e-51 |
| MsG0080049046.01 | MsG0780039979.01 | 0.802889 | 4.528088e-49 | 2.243746e-46 |
| MsG0080049046.01 | MsG0780040497.01 | 0.803057 | 4.180263e-49 | 2.080137e-46 |
| MsG0080049046.01 | MsG0780040498.01 | 0.807591 | 4.690357e-50 | 2.622706e-47 |
| MsG0080049046.01 | MsG0780040560.01 | 0.805418 | 1.347947e-49 | 7.127652e-47 |
| MsG0080049046.01 | MsG0780040598.01 | 0.805572 | 1.251005e-49 | 6.641170e-47 |
| MsG0080049046.01 | MsG0780041036.01 | 0.801658 | 8.116894e-49 | 3.897789e-46 |
| MsG0080049046.01 | MsG0780041180.01 | 0.803408 | 3.535773e-49 | 1.775425e-46 |
| MsG0080049046.01 | MsG0880042122.01 | 0.802026 | 6.821054e-49 | 3.306320e-46 |
| MsG0080049046.01 | MsG0880043212.01 | 0.829718 | 4.401411e-55 | 4.542074e-52 |
| MsG0080049046.01 | MsG0880043906.01 | 0.801641 | 8.182683e-49 | 3.927722e-46 |
| MsG0080049046.01 | MsG0880045854.01 | 0.802092 | 6.609797e-49 | 3.209399e-46 |
| MsG0080049046.01 | MsG0880046095.01 | 0.805036 | 1.620247e-49 | 8.483845e-47 |
| MsG0080049046.01 | MsG0880047383.01 | 0.802952 | 4.394115e-49 | 2.180786e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049046.01.T01 | MTR_1g046490 | 97.447 | 235 | 6 | 0 | 1 | 235 | 2 | 236 | 2.60e-166 | 458 |
| MsG0080049046.01.T01 | MTR_1g046800 | 89.130 | 230 | 23 | 1 | 6 | 235 | 23 | 250 | 1.18e-146 | 409 |
| MsG0080049046.01.T01 | MTR_1g046500 | 89.565 | 230 | 22 | 1 | 6 | 235 | 23 | 250 | 2.29e-142 | 398 |
| MsG0080049046.01.T01 | MTR_4g062470 | 42.982 | 228 | 109 | 6 | 22 | 235 | 84 | 304 | 4.56e-40 | 140 |
| MsG0080049046.01.T01 | MTR_4g062520 | 42.060 | 233 | 113 | 6 | 18 | 235 | 78 | 303 | 5.94e-40 | 139 |
| MsG0080049046.01.T01 | MTR_1g115510 | 38.235 | 238 | 124 | 6 | 18 | 235 | 119 | 353 | 2.20e-38 | 137 |
| MsG0080049046.01.T01 | MTR_7g021300 | 36.719 | 256 | 122 | 8 | 4 | 233 | 146 | 387 | 4.86e-38 | 136 |
| MsG0080049046.01.T01 | MTR_4g062460 | 38.596 | 228 | 120 | 7 | 20 | 233 | 36 | 257 | 5.28e-34 | 123 |
| MsG0080049046.01.T01 | MTR_1g115515 | 27.854 | 219 | 126 | 4 | 22 | 235 | 43 | 234 | 1.75e-17 | 79.0 |
| MsG0080049046.01.T01 | MTR_1g054525 | 35.780 | 109 | 67 | 2 | 103 | 208 | 62 | 170 | 3.09e-15 | 72.0 |
| MsG0080049046.01.T01 | MTR_4g049550 | 37.069 | 116 | 64 | 3 | 121 | 233 | 66 | 175 | 1.07e-14 | 70.1 |
| MsG0080049046.01.T01 | MTR_4g122110 | 37.079 | 89 | 55 | 1 | 119 | 206 | 66 | 154 | 3.09e-14 | 68.9 |
| MsG0080049046.01.T01 | MTR_7g093790 | 41.053 | 95 | 54 | 2 | 118 | 210 | 75 | 169 | 5.99e-14 | 68.6 |
| MsG0080049046.01.T01 | MTR_4g122130 | 39.000 | 100 | 58 | 3 | 113 | 210 | 60 | 158 | 8.16e-14 | 67.8 |
| MsG0080049046.01.T01 | MTR_4g013385 | 40.000 | 90 | 53 | 1 | 121 | 209 | 78 | 167 | 9.76e-14 | 67.8 |
| MsG0080049046.01.T01 | MTR_0433s0040 | 40.000 | 90 | 53 | 1 | 121 | 209 | 78 | 167 | 9.76e-14 | 67.8 |
| MsG0080049046.01.T01 | MTR_7g093820 | 38.947 | 95 | 56 | 2 | 118 | 210 | 78 | 172 | 1.74e-13 | 67.0 |
| MsG0080049046.01.T01 | MTR_7g093850 | 38.947 | 95 | 56 | 2 | 118 | 210 | 78 | 172 | 2.13e-13 | 67.0 |
| MsG0080049046.01.T01 | MTR_4g013335 | 29.927 | 137 | 93 | 2 | 75 | 208 | 36 | 172 | 2.65e-13 | 66.6 |
| MsG0080049046.01.T01 | MTR_7g093830 | 38.947 | 95 | 56 | 2 | 118 | 210 | 77 | 171 | 3.18e-13 | 66.6 |
| MsG0080049046.01.T01 | MTR_4g049570 | 34.211 | 114 | 70 | 2 | 121 | 233 | 66 | 175 | 5.61e-13 | 65.5 |
| MsG0080049046.01.T01 | MTR_4g013310 | 30.147 | 136 | 84 | 3 | 105 | 235 | 67 | 196 | 6.82e-13 | 65.5 |
| MsG0080049046.01.T01 | MTR_4g013325 | 36.667 | 90 | 56 | 1 | 120 | 208 | 86 | 175 | 1.04e-12 | 65.1 |
| MsG0080049046.01.T01 | MTR_4g013770 | 38.298 | 94 | 57 | 1 | 120 | 212 | 78 | 171 | 2.00e-12 | 64.3 |
| MsG0080049046.01.T01 | MTR_3g034030 | 32.407 | 108 | 72 | 1 | 101 | 207 | 53 | 160 | 4.87e-12 | 63.2 |
| MsG0080049046.01.T01 | MTR_4g078885 | 31.481 | 108 | 73 | 1 | 101 | 207 | 53 | 160 | 2.50e-11 | 61.2 |
| MsG0080049046.01.T01 | MTR_7g093870 | 36.082 | 97 | 56 | 2 | 119 | 210 | 76 | 171 | 4.39e-11 | 60.8 |
| MsG0080049046.01.T01 | MTR_4g013345 | 35.955 | 89 | 56 | 1 | 121 | 208 | 84 | 172 | 5.92e-11 | 60.1 |
| MsG0080049046.01.T01 | MTR_5g096120 | 41.304 | 92 | 52 | 1 | 121 | 210 | 76 | 167 | 7.64e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049046.01.T01 | AT4G13580 | 68.803 | 234 | 66 | 2 | 2 | 235 | 18 | 244 | 5.75e-109 | 313 |
| MsG0080049046.01.T01 | AT3G24020 | 67.382 | 233 | 69 | 2 | 3 | 235 | 18 | 243 | 3.98e-108 | 311 |
| MsG0080049046.01.T01 | AT1G07730 | 44.348 | 230 | 106 | 6 | 24 | 235 | 164 | 389 | 1.02e-43 | 151 |
| MsG0080049046.01.T01 | AT3G55230 | 39.918 | 243 | 124 | 7 | 14 | 235 | 62 | 303 | 1.36e-42 | 146 |
| MsG0080049046.01.T01 | AT2G28670 | 46.087 | 230 | 100 | 6 | 26 | 235 | 1 | 226 | 1.95e-42 | 144 |
| MsG0080049046.01.T01 | AT2G28670 | 46.121 | 232 | 101 | 6 | 24 | 235 | 209 | 436 | 1.21e-41 | 147 |
| MsG0080049046.01.T01 | AT2G39430 | 40.496 | 242 | 122 | 8 | 15 | 235 | 80 | 320 | 2.69e-41 | 144 |
| MsG0080049046.01.T01 | AT1G65870 | 33.636 | 110 | 70 | 2 | 107 | 213 | 62 | 171 | 1.20e-14 | 70.5 |
| MsG0080049046.01.T01 | AT5G42510 | 38.043 | 92 | 56 | 1 | 117 | 207 | 69 | 160 | 1.88e-13 | 67.0 |
| MsG0080049046.01.T01 | AT3G13650 | 34.409 | 93 | 60 | 1 | 121 | 212 | 74 | 166 | 2.58e-13 | 66.6 |
| MsG0080049046.01.T01 | AT5G42500 | 35.484 | 93 | 59 | 1 | 119 | 210 | 74 | 166 | 7.90e-13 | 65.1 |
| MsG0080049046.01.T01 | AT3G58090 | 34.091 | 88 | 57 | 1 | 124 | 210 | 163 | 250 | 3.51e-12 | 64.7 |
| MsG0080049046.01.T01 | AT5G49030 | 35.165 | 91 | 58 | 1 | 121 | 210 | 79 | 169 | 4.18e-12 | 65.9 |
| MsG0080049046.01.T01 | AT5G49040 | 35.165 | 91 | 58 | 1 | 121 | 210 | 79 | 169 | 4.19e-11 | 60.5 |
| MsG0080049046.01.T01 | AT1G22900 | 36.364 | 88 | 55 | 1 | 121 | 207 | 84 | 171 | 5.11e-11 | 60.5 |
| MsG0080049046.01.T01 | AT4G38700 | 36.082 | 97 | 57 | 3 | 119 | 210 | 72 | 168 | 7.28e-11 | 60.1 |
Find 70 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTTCCTGTGGCTGCAAT+TGG | 0.276263 | contig634end:+783 | MsG0080049046.01.T01:intergenic |
| CCTACTGTTAATGGTGTTAC+TGG | 0.279068 | contig634end:-588 | MsG0080049046.01.T01:CDS |
| GGATTAACACCATTTGCATT+AGG | 0.297019 | contig634end:+609 | MsG0080049046.01.T01:intergenic |
| AAGTACTATGTCGCGATTAT+CGG | 0.316886 | contig634end:-262 | MsG0080049046.01.T01:CDS |
| GGACCTGATGGACTTGGATT+AGG | 0.319881 | contig634end:-480 | MsG0080049046.01.T01:CDS |
| GATGGACTTGGATTAGGATT+TGG | 0.351208 | contig634end:-474 | MsG0080049046.01.T01:CDS |
| GATTAACACCATTTGCATTA+GGG | 0.356622 | contig634end:+610 | MsG0080049046.01.T01:intergenic |
| GGTGTTACTGGTATCCCTTT+AGG | 0.357291 | contig634end:-576 | MsG0080049046.01.T01:CDS |
| GGTGTTAATCCTACTGTTAA+TGG | 0.373730 | contig634end:-597 | MsG0080049046.01.T01:CDS |
| TTAACATCTCAACCAGAGTT+AGG | 0.377354 | contig634end:-426 | MsG0080049046.01.T01:CDS |
| TGATTGTTATTGTTGTTGTT+TGG | 0.384388 | contig634end:+525 | MsG0080049046.01.T01:intergenic |
| ACTGGTATCCCTTTAGGAAC+TGG | 0.388166 | contig634end:-570 | MsG0080049046.01.T01:CDS |
| GGGAAGTTTAAGTATGCAAA+AGG | 0.390538 | contig634end:-225 | MsG0080049046.01.T01:CDS |
| CTTGGAATGTTGAATCCAAC+TGG | 0.413683 | contig634end:+648 | MsG0080049046.01.T01:intergenic |
| ACAAATTGTTGGAAAGGGTC+AGG | 0.415848 | contig634end:-400 | MsG0080049046.01.T01:CDS |
| ACCTGTCCAGGTGGAATAAG+AGG | 0.418722 | contig634end:+186 | MsG0080049046.01.T01:intergenic |
| TTGTTCATGCATGACATTCT+TGG | 0.430091 | contig634end:-747 | MsG0080049046.01.T01:CDS |
| CCTTTAGGAACTGGTTTAGC+TGG | 0.430422 | contig634end:-561 | MsG0080049046.01.T01:CDS |
| ATACTCATCAGTACTTCAAA+TGG | 0.444661 | contig634end:+124 | MsG0080049046.01.T01:intergenic |
| CCGGTCACTGGTCGAGCCGT+TGG | 0.445896 | contig634end:+714 | MsG0080049046.01.T01:intergenic |
| ACACCATTTGCATTAGGGAT+TGG | 0.448404 | contig634end:+615 | MsG0080049046.01.T01:intergenic |
| ACCTCTTATTCCACCTGGAC+AGG | 0.449379 | contig634end:-187 | MsG0080049046.01.T01:CDS |
| TTATCGGTTGTAGGAGGGAC+AGG | 0.461945 | contig634end:-246 | MsG0080049046.01.T01:CDS |
| CAGCTAAACCAGTTCCTAAA+GGG | 0.469050 | contig634end:+562 | MsG0080049046.01.T01:intergenic |
| AACAATGTTCAGTTAACGTT+AGG | 0.471550 | contig634end:-501 | MsG0080049046.01.T01:CDS |
| GGTTCACAAATTGTTGGAAA+GGG | 0.477487 | contig634end:-405 | MsG0080049046.01.T01:CDS |
| TGCATGAACAACTCAATGAT+TGG | 0.478315 | contig634end:+759 | MsG0080049046.01.T01:intergenic |
| ATGCCAATCCCTAATGCAAA+TGG | 0.485841 | contig634end:-618 | MsG0080049046.01.T01:CDS |
| CGGTCACTGGTCGAGCCGTT+GGG | 0.485879 | contig634end:+715 | MsG0080049046.01.T01:intergenic |
| CAAATTGTTGGAAAGGGTCA+GGG | 0.488459 | contig634end:-399 | MsG0080049046.01.T01:CDS |
| CCAGCTAAACCAGTTCCTAA+AGG | 0.497997 | contig634end:+561 | MsG0080049046.01.T01:intergenic |
| AATCCTAATCCAAGTCCATC+AGG | 0.506312 | contig634end:+477 | MsG0080049046.01.T01:intergenic |
| GAGTTAGGTTCACAAATTGT+TGG | 0.509703 | contig634end:-411 | MsG0080049046.01.T01:CDS |
| CGACCAGTGACCGGTTTGCT+CGG | 0.510231 | contig634end:-705 | MsG0080049046.01.T01:CDS |
| CCTGGACAGGTTGCTACTGA+TGG | 0.513998 | contig634end:-174 | MsG0080049046.01.T01:CDS |
| ATGTAGCAAGTTCGGCTGAC+GGG | 0.514478 | contig634end:-371 | MsG0080049046.01.T01:CDS |
| AGGTTCACAAATTGTTGGAA+AGG | 0.517920 | contig634end:-406 | MsG0080049046.01.T01:CDS |
| ACGTTAGGACCTGATGGACT+TGG | 0.520993 | contig634end:-486 | MsG0080049046.01.T01:CDS |
| CCATCAGTAGCAACCTGTCC+AGG | 0.527354 | contig634end:+174 | MsG0080049046.01.T01:intergenic |
| TCGCGATTATCGGTTGTAGG+AGG | 0.528452 | contig634end:-252 | MsG0080049046.01.T01:CDS |
| GAATCCAACTGGGGTTGCAA+AGG | 0.532298 | contig634end:+659 | MsG0080049046.01.T01:intergenic |
| ACAATTTGTGAACCTAACTC+TGG | 0.539499 | contig634end:+414 | MsG0080049046.01.T01:intergenic |
| ATGTCGCGATTATCGGTTGT+AGG | 0.540679 | contig634end:-255 | MsG0080049046.01.T01:CDS |
| GTATATGTTGCCGAGCAAAC+CGG | 0.541501 | contig634end:+695 | MsG0080049046.01.T01:intergenic |
| GGAAGTTTAAGTATGCAAAA+GGG | 0.542451 | contig634end:-224 | MsG0080049046.01.T01:CDS |
| TTCATGCATGACATTCTTGG+TGG | 0.543393 | contig634end:-744 | MsG0080049046.01.T01:CDS |
| AATCCAACTGGGGTTGCAAA+GGG | 0.544346 | contig634end:+660 | MsG0080049046.01.T01:intergenic |
| AATGATTGGTTCCTTTCCTG+TGG | 0.544898 | contig634end:+773 | MsG0080049046.01.T01:intergenic |
| GGGATTGGCATACTATCACT+TGG | 0.545050 | contig634end:+630 | MsG0080049046.01.T01:intergenic |
| TTGCCGAGCAAACCGGTCAC+TGG | 0.546279 | contig634end:+702 | MsG0080049046.01.T01:intergenic |
| CTGAGACCTCTTATTCCACC+TGG | 0.547547 | contig634end:-192 | MsG0080049046.01.T01:CDS |
| GTACCCTTTGCAACCCCAGT+TGG | 0.549320 | contig634end:-663 | MsG0080049046.01.T01:CDS |
| CGCGATTATCGGTTGTAGGA+GGG | 0.551209 | contig634end:-251 | MsG0080049046.01.T01:CDS |
| TCCAATTGCAGCCACAGGAA+AGG | 0.562874 | contig634end:-784 | MsG0080049046.01.T01:CDS |
| TGACGGGAGTAGACAGATGA+TGG | 0.563922 | contig634end:-355 | MsG0080049046.01.T01:CDS |
| ATTGATCCAATTGCAGCCAC+AGG | 0.569919 | contig634end:-789 | MsG0080049046.01.T01:CDS |
| CCAGTAACACCATTAACAGT+AGG | 0.579036 | contig634end:+588 | MsG0080049046.01.T01:intergenic |
| CAGTTAACGTTAGGACCTGA+TGG | 0.580607 | contig634end:-492 | MsG0080049046.01.T01:CDS |
| AAATTGTTGGAAAGGGTCAG+GGG | 0.581072 | contig634end:-398 | MsG0080049046.01.T01:CDS |
| TATGTAGCAAGTTCGGCTGA+CGG | 0.590795 | contig634end:-372 | MsG0080049046.01.T01:CDS |
| CCAACGGCTCGACCAGTGAC+CGG | 0.606503 | contig634end:-714 | MsG0080049046.01.T01:CDS |
| TTGGAATGTTGAATCCAACT+GGG | 0.618283 | contig634end:+649 | None:intergenic |
| TACCATTGCAATGATGGCAA+TGG | 0.622938 | contig634end:-829 | None:intergenic |
| TCTTGGTGGCAGTAACCCAA+CGG | 0.656099 | contig634end:-730 | MsG0080049046.01.T01:CDS |
| TCAGTAGCAACCTGTCCAGG+TGG | 0.657359 | contig634end:+177 | MsG0080049046.01.T01:intergenic |
| AATTGTTGGAAAGGGTCAGG+GGG | 0.661911 | contig634end:-397 | MsG0080049046.01.T01:CDS |
| TATCGGTTGTAGGAGGGACA+GGG | 0.674182 | contig634end:-245 | MsG0080049046.01.T01:CDS |
| ATGGTGCTGAGACTTTGTTG+AGG | 0.693982 | contig634end:-155 | MsG0080049046.01.T01:CDS |
| TTGCTCGGCAACATATACAG+TGG | 0.727097 | contig634end:-690 | MsG0080049046.01.T01:CDS |
| TGGAATGTTGAATCCAACTG+GGG | 0.746650 | contig634end:+650 | MsG0080049045.01.T01:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTATGGTTTGTATAAGAT+TGG | - | contig634end:662-681 | MsG0080049046.01.T01:CDS | 20.0% |
| !! | GGTGATAGTTTGAATTTTTA+TGG | - | contig634end:647-666 | MsG0080049046.01.T01:CDS | 25.0% |
| !! | TGATTGTTATTGTTGTTGTT+TGG | + | contig634end:425-444 | MsG0080049046.01.T01:intergenic | 25.0% |
| AACAATGTTCAGTTAACGTT+AGG | - | contig634end:446-465 | MsG0080049046.01.T01:CDS | 30.0% | |
| GATTAACACCATTTGCATTA+GGG | + | contig634end:340-359 | MsG0080049046.01.T01:intergenic | 30.0% | |
| GGAAGTTTAAGTATGCAAAA+GGG | - | contig634end:723-742 | MsG0080049046.01.T01:CDS | 30.0% | |
| !! | GTTTTTACAGCACTTTTTGA+AGG | - | contig634end:614-633 | MsG0080049046.01.T01:CDS | 30.0% |
| AAGTACTATGTCGCGATTAT+CGG | - | contig634end:685-704 | MsG0080049046.01.T01:CDS | 35.0% | |
| ACAATTTGTGAACCTAACTC+TGG | + | contig634end:536-555 | MsG0080049046.01.T01:intergenic | 35.0% | |
| AGGTTCACAAATTGTTGGAA+AGG | - | contig634end:541-560 | MsG0080049046.01.T01:CDS | 35.0% | |
| GAGTTAGGTTCACAAATTGT+TGG | - | contig634end:536-555 | MsG0080049046.01.T01:CDS | 35.0% | |
| GGATTAACACCATTTGCATT+AGG | + | contig634end:341-360 | MsG0080049046.01.T01:intergenic | 35.0% | |
| GGGAAGTTTAAGTATGCAAA+AGG | - | contig634end:722-741 | MsG0080049046.01.T01:CDS | 35.0% | |
| GGTGTTAATCCTACTGTTAA+TGG | - | contig634end:350-369 | MsG0080049046.01.T01:CDS | 35.0% | |
| GGTTCACAAATTGTTGGAAA+GGG | - | contig634end:542-561 | MsG0080049046.01.T01:CDS | 35.0% | |
| TGCATGAACAACTCAATGAT+TGG | + | contig634end:191-210 | MsG0080049046.01.T01:intergenic | 35.0% | |
| TTAACATCTCAACCAGAGTT+AGG | - | contig634end:521-540 | MsG0080049046.01.T01:CDS | 35.0% | |
| TTGGAATGTTGAATCCAACT+GGG | + | contig634end:301-320 | MsG0080049046.01.T01:intergenic | 35.0% | |
| TTGTTCATGCATGACATTCT+TGG | - | contig634end:200-219 | MsG0080049046.01.T01:CDS | 35.0% | |
| !! | TTACAGCACTTTTTGAAGGA+GGG | - | contig634end:618-637 | MsG0080049046.01.T01:CDS | 35.0% |
| !! | TTTACAGCACTTTTTGAAGG+AGG | - | contig634end:617-636 | MsG0080049046.01.T01:CDS | 35.0% |
| AAATTGTTGGAAAGGGTCAG+GGG | - | contig634end:549-568 | MsG0080049046.01.T01:CDS | 40.0% | |
| AATCCTAATCCAAGTCCATC+AGG | + | contig634end:473-492 | MsG0080049046.01.T01:intergenic | 40.0% | |
| ACAAATTGTTGGAAAGGGTC+AGG | - | contig634end:547-566 | MsG0080049046.01.T01:CDS | 40.0% | |
| ACACCATTTGCATTAGGGAT+TGG | + | contig634end:335-354 | MsG0080049046.01.T01:intergenic | 40.0% | |
| ATGCCAATCCCTAATGCAAA+TGG | - | contig634end:329-348 | MsG0080049046.01.T01:CDS | 40.0% | |
| CAAATTGTTGGAAAGGGTCA+GGG | - | contig634end:548-567 | MsG0080049046.01.T01:CDS | 40.0% | |
| CAGCTAAACCAGTTCCTAAA+GGG | + | contig634end:388-407 | MsG0080049046.01.T01:intergenic | 40.0% | |
| CCAGTAACACCATTAACAGT+AGG | + | contig634end:362-381 | MsG0080049046.01.T01:intergenic | 40.0% | |
| CTTGGAATGTTGAATCCAAC+TGG | + | contig634end:302-321 | MsG0080049046.01.T01:intergenic | 40.0% | |
| TGGAATGTTGAATCCAACTG+GGG | + | contig634end:300-319 | MsG0080049046.01.T01:intergenic | 40.0% | |
| TTCATGCATGACATTCTTGG+TGG | - | contig634end:203-222 | MsG0080049046.01.T01:CDS | 40.0% | |
| ! | AATGATTGGTTCCTTTCCTG+TGG | + | contig634end:177-196 | MsG0080049046.01.T01:intergenic | 40.0% |
| ! | GATGGACTTGGATTAGGATT+TGG | - | contig634end:473-492 | MsG0080049046.01.T01:CDS | 40.0% |
| !! | CCTACTGTTAATGGTGTTAC+TGG | - | contig634end:359-378 | MsG0080049046.01.T01:CDS | 40.0% |
| !! | TACAGCACTTTTTGAAGGAG+GGG | - | contig634end:619-638 | MsG0080049046.01.T01:CDS | 40.0% |
| AATCCAACTGGGGTTGCAAA+GGG | + | contig634end:290-309 | MsG0080049046.01.T01:intergenic | 45.0% | |
| AATTGTTGGAAAGGGTCAGG+GGG | - | contig634end:550-569 | MsG0080049046.01.T01:CDS | 45.0% | |
| ATTGATCCAATTGCAGCCAC+AGG | - | contig634end:158-177 | MsG0080049046.01.T01:CDS | 45.0% | |
| CAGTTAACGTTAGGACCTGA+TGG | - | contig634end:455-474 | MsG0080049046.01.T01:CDS | 45.0% | |
| CCAGCTAAACCAGTTCCTAA+AGG | + | contig634end:389-408 | MsG0080049046.01.T01:intergenic | 45.0% | |
| GTATATGTTGCCGAGCAAAC+CGG | + | contig634end:255-274 | MsG0080049046.01.T01:intergenic | 45.0% | |
| TATGTAGCAAGTTCGGCTGA+CGG | - | contig634end:575-594 | MsG0080049046.01.T01:CDS | 45.0% | |
| TTGCTCGGCAACATATACAG+TGG | - | contig634end:257-276 | MsG0080049046.01.T01:CDS | 45.0% | |
| ! | ACTGGTATCCCTTTAGGAAC+TGG | - | contig634end:377-396 | MsG0080049046.01.T01:CDS | 45.0% |
| ! | ATGGTGCTGAGACTTTGTTG+AGG | - | contig634end:792-811 | MsG0080049046.01.T01:CDS | 45.0% |
| ! | ATGTCGCGATTATCGGTTGT+AGG | - | contig634end:692-711 | MsG0080049046.01.T01:CDS | 45.0% |
| ! | CCTTTAGGAACTGGTTTAGC+TGG | - | contig634end:386-405 | MsG0080049046.01.T01:CDS | 45.0% |
| ! | GGGATTGGCATACTATCACT+TGG | + | contig634end:320-339 | MsG0080049046.01.T01:intergenic | 45.0% |
| !! | CTTTTTGAAGGAGGGGAGTA+TGG | - | contig634end:626-645 | MsG0080049046.01.T01:CDS | 45.0% |
| !! | GGTGTTACTGGTATCCCTTT+AGG | - | contig634end:371-390 | MsG0080049046.01.T01:CDS | 45.0% |
| ACCTCTTATTCCACCTGGAC+AGG | - | contig634end:760-779 | MsG0080049046.01.T01:CDS | 50.0% | |
| ACCTGTCCAGGTGGAATAAG+AGG | + | contig634end:764-783 | MsG0080049046.01.T01:intergenic | 50.0% | |
| ATGTAGCAAGTTCGGCTGAC+GGG | - | contig634end:576-595 | MsG0080049046.01.T01:CDS | 50.0% | |
| CTGAGACCTCTTATTCCACC+TGG | - | contig634end:755-774 | MsG0080049046.01.T01:CDS | 50.0% | |
| GAATCCAACTGGGGTTGCAA+AGG | + | contig634end:291-310 | MsG0080049046.01.T01:intergenic | 50.0% | |
| TCCAATTGCAGCCACAGGAA+AGG | - | contig634end:163-182 | MsG0080049046.01.T01:CDS | 50.0% | |
| TCCTTTCCTGTGGCTGCAAT+TGG | + | contig634end:167-186 | MsG0080049046.01.T01:intergenic | 50.0% | |
| TGACGGGAGTAGACAGATGA+TGG | - | contig634end:592-611 | MsG0080049046.01.T01:CDS | 50.0% | |
| ! | CGCGATTATCGGTTGTAGGA+GGG | - | contig634end:696-715 | MsG0080049046.01.T01:CDS | 50.0% |
| ! | GGGGGTGTATGTAGCAAGTT+CGG | - | contig634end:568-587 | MsG0080049046.01.T01:CDS | 50.0% |
| ! | TATCGGTTGTAGGAGGGACA+GGG | - | contig634end:702-721 | MsG0080049046.01.T01:CDS | 50.0% |
| ! | TCGCGATTATCGGTTGTAGG+AGG | - | contig634end:695-714 | MsG0080049046.01.T01:CDS | 50.0% |
| ! | TCTTGGTGGCAGTAACCCAA+CGG | - | contig634end:217-236 | MsG0080049046.01.T01:CDS | 50.0% |
| ! | TTATCGGTTGTAGGAGGGAC+AGG | - | contig634end:701-720 | MsG0080049046.01.T01:CDS | 50.0% |
| !! | ACGTTAGGACCTGATGGACT+TGG | - | contig634end:461-480 | MsG0080049046.01.T01:CDS | 50.0% |
| !! | GGACCTGATGGACTTGGATT+AGG | - | contig634end:467-486 | MsG0080049046.01.T01:CDS | 50.0% |
| CCATCAGTAGCAACCTGTCC+AGG | + | contig634end:776-795 | MsG0080049046.01.T01:intergenic | 55.0% | |
| CCTGGACAGGTTGCTACTGA+TGG | - | contig634end:773-792 | MsG0080049046.01.T01:CDS | 55.0% | |
| GTACCCTTTGCAACCCCAGT+TGG | - | contig634end:284-303 | MsG0080049046.01.T01:CDS | 55.0% | |
| TCAGTAGCAACCTGTCCAGG+TGG | + | contig634end:773-792 | MsG0080049046.01.T01:intergenic | 55.0% | |
| TTGCCGAGCAAACCGGTCAC+TGG | + | contig634end:248-267 | MsG0080049046.01.T01:intergenic | 60.0% | |
| !! | CGACCAGTGACCGGTTTGCT+CGG | - | contig634end:242-261 | MsG0080049046.01.T01:CDS | 60.0% |
| CCAACGGCTCGACCAGTGAC+CGG | - | contig634end:233-252 | MsG0080049046.01.T01:CDS | 65.0% | |
| CGGTCACTGGTCGAGCCGTT+GGG | + | contig634end:235-254 | MsG0080049046.01.T01:intergenic | 65.0% | |
| CCGGTCACTGGTCGAGCCGT+TGG | + | contig634end:236-255 | MsG0080049046.01.T01:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig634end | gene | 131 | 838 | 131 | ID=MsG0080049046.01;Name=MsG0080049046.01 |
| contig634end | mRNA | 131 | 838 | 131 | ID=MsG0080049046.01.T01;Parent=MsG0080049046.01;Name=MsG0080049046.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|235 |
| contig634end | exon | 131 | 838 | 131 | ID=MsG0080049046.01.T01:exon:42150;Parent=MsG0080049046.01.T01 |
| contig634end | CDS | 131 | 838 | 131 | ID=MsG0080049046.01.T01:cds;Parent=MsG0080049046.01.T01 |
| Gene Sequence |
| Protein sequence |
