Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049071.01.T01 | XP_003631129.1 | 98.193 | 166 | 3 | 0 | 1 | 166 | 1 | 166 | 4.61E-118 | 340 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049071.01.T01 | G7LE73 | 98.193 | 166 | 3 | 0 | 1 | 166 | 1 | 166 | 2.20e-118 | 340 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049071.01 | MsG0180000367.01 | 0.820005 | 8.618217e-53 | 6.738079e-50 |
| MsG0080049071.01 | MsG0380012889.01 | 0.808354 | 3.227220e-50 | 1.841312e-47 |
| MsG0080049071.01 | MsG0380014410.01 | 0.819692 | 1.016500e-52 | 7.877668e-50 |
| MsG0080049071.01 | MsG0480023429.01 | 0.818375 | 2.025595e-52 | 1.513727e-49 |
| MsG0080049071.01 | MsG0480023987.01 | 0.803194 | 3.916180e-49 | 1.955714e-46 |
| MsG0080049071.01 | MsG0580028302.01 | 0.800525 | 1.383510e-48 | 6.456184e-46 |
| MsG0080049071.01 | MsG0580029240.01 | 0.802736 | 4.869228e-49 | 2.403451e-46 |
| MsG0080049071.01 | MsG0780038307.01 | 0.805783 | 1.129826e-49 | 6.030842e-47 |
| MsG0080049071.01 | MsG0780038519.01 | 0.803193 | 3.918464e-49 | 1.956788e-46 |
| MsG0080049071.01 | MsG0780039883.01 | 0.804311 | 2.295797e-49 | 1.179854e-46 |
| MsG0080049071.01 | MsG0780039916.01 | 0.809096 | 2.239716e-50 | 1.303093e-47 |
| MsG0080049071.01 | MsG0780040806.01 | 0.800654 | 1.302033e-48 | 6.096030e-46 |
| MsG0080049071.01 | MsG0880042122.01 | 0.821769 | 3.385679e-53 | 2.780298e-50 |
| MsG0080049071.01 | MsG0880045703.01 | -0.803077 | 4.140323e-49 | 2.061312e-46 |
| MsG0080049071.01 | MsG0880046932.01 | 0.805155 | 1.529829e-49 | 8.035149e-47 |
| MsG0080049071.01 | MsG0880047714.01 | 0.800620 | 1.323222e-48 | 6.189765e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049071.01.T01 | MTR_8g107420 | 98.193 | 166 | 3 | 0 | 1 | 166 | 1 | 166 | 5.58e-122 | 340 |
| MsG0080049071.01.T01 | MTR_1g108640 | 33.708 | 178 | 98 | 4 | 1 | 165 | 1 | 171 | 3.19e-17 | 75.1 |
| MsG0080049071.01.T01 | MTR_2g105800 | 38.384 | 99 | 56 | 1 | 14 | 112 | 15 | 108 | 1.74e-15 | 70.1 |
| MsG0080049071.01.T01 | MTR_5g018720 | 30.303 | 99 | 68 | 1 | 1 | 98 | 1 | 99 | 5.54e-13 | 63.5 |
| MsG0080049071.01.T01 | MTR_1g087230 | 27.273 | 176 | 113 | 4 | 1 | 161 | 1 | 176 | 3.64e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049071.01.T01 | AT1G18290 | 37.017 | 181 | 90 | 7 | 1 | 162 | 1 | 176 | 1.31e-30 | 109 |
| MsG0080049071.01.T01 | AT5G66580 | 34.524 | 84 | 55 | 0 | 15 | 98 | 16 | 99 | 5.98e-13 | 63.2 |
| MsG0080049071.01.T01 | AT4G37240 | 34.343 | 99 | 64 | 1 | 1 | 98 | 1 | 99 | 1.16e-12 | 62.8 |
| MsG0080049071.01.T01 | AT3G03280 | 30.539 | 167 | 105 | 4 | 1 | 161 | 1 | 162 | 2.33e-12 | 62.0 |
| MsG0080049071.01.T01 | AT2G23690 | 33.333 | 84 | 56 | 0 | 15 | 98 | 16 | 99 | 6.73e-11 | 58.2 |
Find 42 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATGCGAGTTTCAACAATT+TGG | 0.189626 | contig647end:+24384 | MsG0080049071.01.T01:CDS |
| TCCAACCAAAGGGGTGTTTA+AGG | 0.257462 | contig647end:+24108 | MsG0080049071.01.T01:CDS |
| TTTAAGGTGCTACTCTTAGA+TGG | 0.302251 | contig647end:+24124 | MsG0080049071.01.T01:CDS |
| CATGCGAGTTTCAACAATTT+GGG | 0.302990 | contig647end:+24385 | MsG0080049071.01.T01:CDS |
| GGGAGGCTGAACCTGTTAAA+AGG | 0.353755 | contig647end:+24497 | MsG0080049071.01.T01:CDS |
| TAAGAGCACTCATTTCTTCA+TGG | 0.366168 | contig647end:-24336 | None:intergenic |
| AGTTTCATTTATTGCCTTGA+TGG | 0.393034 | contig647end:-24571 | None:intergenic |
| GGTACTCCAAGCAGAGATCT+TGG | 0.420093 | contig647end:+24518 | MsG0080049071.01.T01:CDS |
| GAATTCGATGGCCAACTTTG+AGG | 0.420836 | contig647end:-24231 | None:intergenic |
| AAAGTTGGCCATCGAATTCA+AGG | 0.441588 | contig647end:+24235 | MsG0080049071.01.T01:CDS |
| CCTTGATGGAGTTTCATCAA+TGG | 0.445048 | contig647end:-24557 | None:intergenic |
| TGAGTTCTGCATGTTCCCTT+CGG | 0.456879 | contig647end:+24429 | MsG0080049071.01.T01:CDS |
| ACCTTAAACACCCCTTTGGT+TGG | 0.462577 | contig647end:-24109 | None:intergenic |
| TAGCACCTTAAACACCCCTT+TGG | 0.471231 | contig647end:-24113 | None:intergenic |
| CTGATAATTATGAGATGCTA+AGG | 0.473011 | contig647end:+24476 | MsG0080049071.01.T01:CDS |
| AAGAGCACTCATTTCTTCAT+GGG | 0.480087 | contig647end:-24335 | None:intergenic |
| CTTCTTGTACTCCAACCAAA+GGG | 0.491778 | contig647end:+24098 | MsG0080049071.01.T01:CDS |
| GCGTGTTTCAAGTTCTTCAT+CGG | 0.493614 | contig647end:-24266 | None:intergenic |
| TCATCAATGGTCTCCAATGC+AGG | 0.508093 | contig647end:-24544 | None:intergenic |
| GAGTCACAAACAAACTGTCC+AGG | 0.528192 | contig647end:-24202 | None:intergenic |
| ATTAAGAGCTGCAGAGCTGA+TGG | 0.532721 | contig647end:+24171 | MsG0080049071.01.T01:CDS |
| GACTCAAGCCACCTCAAAGT+TGG | 0.535144 | contig647end:+24220 | MsG0080049071.01.T01:CDS |
| GAGATCTTGGAGACCTGCAT+TGG | 0.537320 | contig647end:+24531 | MsG0080049071.01.T01:CDS |
| GGGTTAACACAGAGTATAGC+AGG | 0.538805 | contig647end:-24315 | None:intergenic |
| ATTGTTGAAACTCGCATGCT+TGG | 0.542497 | contig647end:-24380 | None:intergenic |
| TTAAGGTGCTACTCTTAGAT+GGG | 0.544485 | contig647end:+24125 | MsG0080049071.01.T01:CDS |
| GTTGAAACTCGCATGCTTGG+TGG | 0.552836 | contig647end:-24377 | None:intergenic |
| ACAGAGTATAGCAGGTCCAT+TGG | 0.559102 | contig647end:-24307 | None:intergenic |
| ACTCTTAGATGGGAGAATAG+AGG | 0.565120 | contig647end:+24135 | MsG0080049071.01.T01:CDS |
| TTCGATGGCCAACTTTGAGG+TGG | 0.568058 | contig647end:-24228 | None:intergenic |
| CTAATAGTCCTTGAATTCGA+TGG | 0.579984 | contig647end:-24243 | None:intergenic |
| GTTCTGCATGTTCCCTTCGG+AGG | 0.580244 | contig647end:+24432 | MsG0080049071.01.T01:CDS |
| CTCTTAACTACAAAGCGTCA+AGG | 0.588617 | contig647end:+24353 | MsG0080049071.01.T01:CDS |
| GCTTCTTGTACTCCAACCAA+AGG | 0.589757 | contig647end:+24097 | MsG0080049071.01.T01:CDS |
| TCTTAACTACAAAGCGTCAA+GGG | 0.619592 | contig647end:+24354 | MsG0080049071.01.T01:CDS |
| GAGCTGATGGTAGAATACCC+TGG | 0.631828 | contig647end:+24184 | MsG0080049071.01.T01:CDS |
| CCATTGATGAAACTCCATCA+AGG | 0.632248 | contig647end:+24557 | MsG0080049071.01.T01:CDS |
| AGTCACAAACAAACTGTCCA+GGG | 0.643975 | contig647end:-24201 | None:intergenic |
| TGATAATTATGAGATGCTAA+GGG | 0.645988 | contig647end:+24477 | MsG0080049071.01.T01:CDS |
| AGGTCTCCAAGATCTCTGCT+TGG | 0.669783 | contig647end:-24524 | None:intergenic |
| TAATTATGAGATGCTAAGGG+AGG | 0.689423 | contig647end:+24480 | MsG0080049071.01.T01:CDS |
| TTCTTGTACTCCAACCAAAG+GGG | 0.691807 | contig647end:+24099 | MsG0080049071.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATAATTATGAGATGCTAA+GGG | + | contig647end:24477-24496 | MsG0080049071.01.T01:CDS | 25.0% |
| CTGATAATTATGAGATGCTA+AGG | + | contig647end:24476-24495 | MsG0080049071.01.T01:CDS | 30.0% | |
| ! | GTTTTACTTTCTTCTACCAA+TGG | + | contig647end:24291-24310 | MsG0080049071.01.T01:CDS | 30.0% |
| ATGCAGAACTCACTAAAAAC+AGG | - | contig647end:24421-24440 | None:intergenic | 35.0% | |
| CATGCGAGTTTCAACAATTT+GGG | + | contig647end:24385-24404 | MsG0080049071.01.T01:CDS | 35.0% | |
| CTAATAGTCCTTGAATTCGA+TGG | - | contig647end:24246-24265 | None:intergenic | 35.0% | |
| TAATTATGAGATGCTAAGGG+AGG | + | contig647end:24480-24499 | MsG0080049071.01.T01:CDS | 35.0% | |
| ! | TCTTAACTACAAAGCGTCAA+GGG | + | contig647end:24354-24373 | MsG0080049071.01.T01:CDS | 35.0% |
| !! | AAGAGCACTCATTTCTTCAT+GGG | - | contig647end:24338-24357 | None:intergenic | 35.0% |
| !! | TAAGAGCACTCATTTCTTCA+TGG | - | contig647end:24339-24358 | None:intergenic | 35.0% |
| !! | TTAAGGTGCTACTCTTAGAT+GGG | + | contig647end:24125-24144 | MsG0080049071.01.T01:CDS | 35.0% |
| !! | TTTAAGGTGCTACTCTTAGA+TGG | + | contig647end:24124-24143 | MsG0080049071.01.T01:CDS | 35.0% |
| AAAGTTGGCCATCGAATTCA+AGG | + | contig647end:24235-24254 | MsG0080049071.01.T01:CDS | 40.0% | |
| ACTCTTAGATGGGAGAATAG+AGG | + | contig647end:24135-24154 | MsG0080049071.01.T01:CDS | 40.0% | |
| AGTCACAAACAAACTGTCCA+GGG | - | contig647end:24204-24223 | None:intergenic | 40.0% | |
| ATTGTTGAAACTCGCATGCT+TGG | - | contig647end:24383-24402 | None:intergenic | 40.0% | |
| CCATTGATGAAACTCCATCA+AGG | + | contig647end:24557-24576 | MsG0080049071.01.T01:CDS | 40.0% | |
| CCTTGATGGAGTTTCATCAA+TGG | - | contig647end:24560-24579 | None:intergenic | 40.0% | |
| CTTCTTGTACTCCAACCAAA+GGG | + | contig647end:24098-24117 | MsG0080049071.01.T01:CDS | 40.0% | |
| GCATGCGAGTTTCAACAATT+TGG | + | contig647end:24384-24403 | MsG0080049071.01.T01:CDS | 40.0% | |
| TTCTTGTACTCCAACCAAAG+GGG | + | contig647end:24099-24118 | MsG0080049071.01.T01:CDS | 40.0% | |
| ! | ACTATTTTCTTTGCCTCCGA+AGG | - | contig647end:24448-24467 | None:intergenic | 40.0% |
| ! | CTATTTTCTTTGCCTCCGAA+GGG | - | contig647end:24447-24466 | None:intergenic | 40.0% |
| ! | CTCTTAACTACAAAGCGTCA+AGG | + | contig647end:24353-24372 | MsG0080049071.01.T01:CDS | 40.0% |
| !! | GCGTGTTTCAAGTTCTTCAT+CGG | - | contig647end:24269-24288 | None:intergenic | 40.0% |
| !! | TGCTTGGAGTACCTTTTAAC+AGG | - | contig647end:24511-24530 | None:intergenic | 40.0% |
| ACAGAGTATAGCAGGTCCAT+TGG | - | contig647end:24310-24329 | None:intergenic | 45.0% | |
| ACCTTAAACACCCCTTTGGT+TGG | - | contig647end:24112-24131 | None:intergenic | 45.0% | |
| ATTAAGAGCTGCAGAGCTGA+TGG | + | contig647end:24171-24190 | MsG0080049071.01.T01:CDS | 45.0% | |
| GAATTCGATGGCCAACTTTG+AGG | - | contig647end:24234-24253 | None:intergenic | 45.0% | |
| GAGTCACAAACAAACTGTCC+AGG | - | contig647end:24205-24224 | None:intergenic | 45.0% | |
| GCTTCTTGTACTCCAACCAA+AGG | + | contig647end:24097-24116 | MsG0080049071.01.T01:CDS | 45.0% | |
| GGGTTAACACAGAGTATAGC+AGG | - | contig647end:24318-24337 | None:intergenic | 45.0% | |
| TAGCACCTTAAACACCCCTT+TGG | - | contig647end:24116-24135 | None:intergenic | 45.0% | |
| TCATCAATGGTCTCCAATGC+AGG | - | contig647end:24547-24566 | None:intergenic | 45.0% | |
| TGAGTTCTGCATGTTCCCTT+CGG | + | contig647end:24429-24448 | MsG0080049071.01.T01:CDS | 45.0% | |
| !! | TCCAACCAAAGGGGTGTTTA+AGG | + | contig647end:24108-24127 | MsG0080049071.01.T01:CDS | 45.0% |
| AGGTCTCCAAGATCTCTGCT+TGG | - | contig647end:24527-24546 | None:intergenic | 50.0% | |
| GACTCAAGCCACCTCAAAGT+TGG | + | contig647end:24220-24239 | MsG0080049071.01.T01:CDS | 50.0% | |
| GAGATCTTGGAGACCTGCAT+TGG | + | contig647end:24531-24550 | MsG0080049071.01.T01:CDS | 50.0% | |
| GAGCTGATGGTAGAATACCC+TGG | + | contig647end:24184-24203 | MsG0080049071.01.T01:CDS | 50.0% | |
| GGGAGGCTGAACCTGTTAAA+AGG | + | contig647end:24497-24516 | MsG0080049071.01.T01:CDS | 50.0% | |
| GGTACTCCAAGCAGAGATCT+TGG | + | contig647end:24518-24537 | MsG0080049071.01.T01:CDS | 50.0% | |
| GTTGAAACTCGCATGCTTGG+TGG | - | contig647end:24380-24399 | None:intergenic | 50.0% | |
| ! | TTCGATGGCCAACTTTGAGG+TGG | - | contig647end:24231-24250 | None:intergenic | 50.0% |
| GTTCTGCATGTTCCCTTCGG+AGG | + | contig647end:24432-24451 | MsG0080049071.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig647end | gene | 24085 | 24585 | 24085 | ID=MsG0080049071.01;Name=MsG0080049071.01 |
| contig647end | mRNA | 24085 | 24585 | 24085 | ID=MsG0080049071.01.T01;Parent=MsG0080049071.01;Name=MsG0080049071.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|166 |
| contig647end | exon | 24085 | 24585 | 24085 | ID=MsG0080049071.01.T01:exon:13041;Parent=MsG0080049071.01.T01 |
| contig647end | CDS | 24085 | 24585 | 24085 | ID=MsG0080049071.01.T01:cds;Parent=MsG0080049071.01.T01 |
| Gene Sequence |
| Protein sequence |
