AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000041.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000041.01.T01 MTR_1g025620 88.333 360 39 1 1 360 1 357 0.0 545
MsG0180000041.01.T01 MTR_1g034990 57.735 362 84 9 1 360 1 295 1.06e-110 325
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 109 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCTCCTCTCTTTCTTTC+TGG -0.009349 1:-702621 None:intergenic
CCTCTCCTTCCTCTCTTTCT+TGG 0.132787 1:-702677 None:intergenic
CTTGATCGACGATTCGATTA+AGG 0.202790 1:+701611 MsG0180000041.01.T01:CDS
CTTTGTGCGATTCATATCTT+AGG 0.236572 1:+701427 MsG0180000041.01.T01:five_prime_UTR
GGTCTCGGCTTTCTTAATAC+TGG 0.271591 1:-703331 None:intergenic
GGACCCAATTCATATGTTAT+TGG 0.278702 1:-703504 None:intergenic
ACCCTAACTTTCTCTCTCTA+AGG 0.285078 1:+701509 MsG0180000041.01.T01:five_prime_UTR
TCAGCCAATAACATATGAAT+TGG 0.306059 1:+703500 MsG0180000041.01.T01:CDS
ATCCTTAGAGAGAGAAAGTT+AGG 0.323553 1:-701511 None:intergenic
TTTAAAGATCCGTTTGGATT+TGG 0.329981 1:+702591 MsG0180000041.01.T01:intron
CCCCAATTGAAATCCTTGTT+TGG 0.330324 1:-703148 None:intergenic
CGGCAATGGTTTACAGACTT+TGG 0.340853 1:-703382 None:intergenic
GGCAATGGTTTACAGACTTT+GGG 0.346273 1:-703381 None:intergenic
GAGACGGGCATTGTTTGTTT+TGG 0.346686 1:-703558 None:intergenic
GGAGAGGAGGAGGGAGAAGA+AGG 0.356929 1:+702693 MsG0180000041.01.T01:CDS
AAGAGAGGAAGGAGAGGAGG+AGG 0.364104 1:+702683 MsG0180000041.01.T01:CDS
ATCCAAACAAGGATTTCAAT+TGG 0.379094 1:+703146 MsG0180000041.01.T01:CDS
AAGCTTTCTCACCATGATCT+TGG 0.386914 1:-702993 None:intergenic
TCCAAACAAGGATTTCAATT+GGG 0.393476 1:+703147 MsG0180000041.01.T01:CDS
ATATACCCTGGAGGTGGAAA+AGG 0.398371 1:-701570 None:intergenic
GAATTTCCATGGAGCTGACT+TGG 0.402492 1:-703354 None:intergenic
ACTTCTCATGCAAGTGTTAG+TGG 0.404231 1:+702769 MsG0180000041.01.T01:CDS
GAGGGAGAAGAAGGAGAAGA+AGG 0.410322 1:+702702 MsG0180000041.01.T01:CDS
TGGGTCCATTGGATCAAGAT+TGG 0.416330 1:+703520 MsG0180000041.01.T01:CDS
ATCTTCTGTTTCTTTGAGAC+GGG 0.430208 1:-703573 None:intergenic
CTCAAAGCAGTAAGAGGAAA+CGG 0.435836 1:+702953 MsG0180000041.01.T01:CDS
TCAAAGCAGTAAGAGGAAAC+GGG 0.439395 1:+702954 MsG0180000041.01.T01:CDS
ATGGAGCTGACTTGGGGTCT+CGG 0.439706 1:-703346 None:intergenic
AATCAAAATGGAACTGTGTA+TGG 0.440293 1:-703672 None:intergenic
AACATATGAATTGGGTCCAT+TGG 0.441455 1:+703509 MsG0180000041.01.T01:CDS
AATCCTTGTTTGGATTCCTC+AGG 0.444763 1:-703138 None:intergenic
CGGATACAACAGCTGGTGTC+TGG 0.453427 1:-703882 None:intergenic
GTCTCGGCTTTCTTAATACT+GGG 0.457490 1:-703330 None:intergenic
CTTTGGGAGCAGAATTTCCA+TGG 0.462196 1:-703365 None:intergenic
CAAAATCTCTATTGCAACCT+CGG 0.463974 1:+703613 MsG0180000041.01.T01:CDS
CAGGACCTGCCATTCGAATC+CGG 0.464929 1:+703091 MsG0180000041.01.T01:intron
AATTTCCATGGAGCTGACTT+GGG 0.466631 1:-703353 None:intergenic
GTGTTGCAGATTTGCTTGCT+CGG 0.468663 1:+703280 MsG0180000041.01.T01:CDS
CTTAATCGAATCGTCGATCA+AGG 0.469381 1:-701610 None:intergenic
TGGTGCTTTAAAGATCCGTT+TGG 0.471581 1:+702585 MsG0180000041.01.T01:intron
AGGAGAAGGACAAGAAGGAC+AGG 0.477194 1:+702635 MsG0180000041.01.T01:CDS
GCATCAACAGGAACCACATT+CGG 0.477431 1:-703402 None:intergenic
ATAGTTCTCAAAGCAGTAAG+AGG 0.481192 1:+702947 MsG0180000041.01.T01:CDS
GGTTCGCGATGTTTCCTCAA+TGG 0.481505 1:-703117 None:intergenic
AATCTTCTGTTTCTTTGAGA+CGG 0.492372 1:-703574 None:intergenic
CAAGCTCAATAATGAAGTAA+AGG 0.495123 1:+702801 MsG0180000041.01.T01:CDS
GCAACACCAACACTTCTTGA+CGG 0.496396 1:-703264 None:intergenic
AGAGAGGAAGGAGAGGAGGA+GGG 0.499942 1:+702684 MsG0180000041.01.T01:CDS
CAGCCAATAACATATGAATT+GGG 0.502764 1:+703501 MsG0180000041.01.T01:CDS
GGCTCTTGAGGAGAACAAAC+AGG 0.508381 1:-702913 None:intergenic
AGTTGCAGATTTGCTTGCTC+GGG 0.509390 1:+703191 MsG0180000041.01.T01:CDS
TTGAGAACTATCAGAGGAGT+AGG 0.510918 1:-702935 None:intergenic
GCTGCTGCTACAGCATCAAC+AGG 0.513129 1:-703414 None:intergenic
CCAAACAAGGATTTCAATTG+GGG 0.515684 1:+703148 MsG0180000041.01.T01:CDS
TCAGAGGAGTAGGGCTCTTG+AGG 0.517781 1:-702925 None:intergenic
CCAGAAAGAAAGAGAGGAGA+AGG 0.518821 1:+702621 MsG0180000041.01.T01:CDS
TCCTTAGAGAGAGAAAGTTA+GGG 0.529291 1:-701510 None:intergenic
AAAATCTCTATTGCAACCTC+GGG 0.530765 1:+703614 MsG0180000041.01.T01:CDS
CAGGAAAGCCGCTGAGAAAG+CGG 0.531019 1:+702654 MsG0180000041.01.T01:CDS
ATCGTCGATCAAGGAAGAAT+CGG 0.533316 1:-701601 None:intergenic
AGCGGCCAAGAAAGAGAGGA+AGG 0.537304 1:+702672 MsG0180000041.01.T01:CDS
AATAACCAATCTTGATCCAA+TGG 0.537400 1:-703525 None:intergenic
GAGTTGCAGATTTGCTTGCT+CGG 0.538522 1:+703190 MsG0180000041.01.T01:CDS
AATGCATATATGTCAGCCTC+AGG 0.539207 1:-703648 None:intergenic
AACAGGTTGCTCAAGTTCCT+CGG 0.539708 1:-702896 None:intergenic
AAGAGAGGAGAAGGACAAGA+AGG 0.543547 1:+702630 MsG0180000041.01.T01:CDS
TTAGTATATAGTACATTCAT+CGG 0.549861 1:-703902 None:intergenic
TTCGAATCCGGCTTCCATTG+AGG 0.550158 1:+703103 MsG0180000041.01.T01:CDS
CTGTAAACCATTGCCGAATG+TGG 0.550168 1:+703389 MsG0180000041.01.T01:CDS
TATTGGCTGAATGTGAAGTA+AGG 0.551252 1:-703487 None:intergenic
GGAAGCCGGATTCGAATGGC+AGG 0.554110 1:-703096 None:intergenic
TGATGATGAAAGTCGAAGGA+TGG 0.554737 1:+703449 MsG0180000041.01.T01:CDS
CCAAGAAAGAGAGGAAGGAG+AGG 0.563149 1:+702677 MsG0180000041.01.T01:CDS
TGTTGCAGATTTGCTTGCTC+GGG 0.564182 1:+703281 MsG0180000041.01.T01:CDS
ATGAGAATGAACAACTTGAA+AGG 0.565109 1:+702863 MsG0180000041.01.T01:CDS
TATCTGTCACAATGAAATGT+TGG 0.565238 1:-703802 None:intergenic
TATACCCTGGAGGTGGAAAA+GGG 0.565421 1:-701569 None:intergenic
GAGTTACCTAGCCAAGATCA+TGG 0.567970 1:+702982 MsG0180000041.01.T01:CDS
GGAGCTTCTCCAAATCCAAA+CGG 0.568605 1:-702600 None:intergenic
TGCTTGATATACCCTGGAGG+TGG 0.572562 1:-701576 None:intergenic
GCTTTCTTAATACTGGGCAA+TGG 0.573806 1:-703324 None:intergenic
AGAAAGAGAGGAAGGAGAGG+AGG 0.573898 1:+702680 MsG0180000041.01.T01:CDS
ATTTCCATGGAGCTGACTTG+GGG 0.574695 1:-703352 None:intergenic
GATGTTTCCTCAATGGAAGC+CGG 0.576850 1:-703110 None:intergenic
GGGTCATGTTCGAGAAGTGT+AGG 0.577598 1:+703168 MsG0180000041.01.T01:CDS
TTCTCACCATGATCTTGGCT+AGG 0.578085 1:-702988 None:intergenic
CAATGGAAGCCGGATTCGAA+TGG 0.579966 1:-703100 None:intergenic
AGAAAGCGGCCAAGAAAGAG+AGG 0.585778 1:+702668 MsG0180000041.01.T01:CDS
CATTCATCGGATACAACAGC+TGG 0.593784 1:-703889 None:intergenic
TCGGTATGCTTGATATACCC+TGG 0.595782 1:-701582 None:intergenic
ACAGGAACCACATTCGGCAA+TGG 0.596306 1:-703396 None:intergenic
GAGACCCCAAGTCAGCTCCA+TGG 0.601830 1:+703348 MsG0180000041.01.T01:CDS
AGACAGTGCGAAGTTGCGTA+AGG 0.603959 1:+702831 MsG0180000041.01.T01:CDS
AAAAGGGAAGCAACGAGACA+TGG 0.608119 1:-701553 None:intergenic
GAACCTGAGGAATCCAAACA+AGG 0.615001 1:+703135 MsG0180000041.01.T01:CDS
TGAGAACTATCAGAGGAGTA+GGG 0.618458 1:-702934 None:intergenic
GAGACACCGTCAAGAAGTGT+TGG 0.622226 1:+703258 MsG0180000041.01.T01:CDS
CAGTTGATGATGAAAGTCGA+AGG 0.625263 1:+703445 MsG0180000041.01.T01:CDS
GAAGCTCCAGAAAGAAAGAG+AGG 0.632197 1:+702615 MsG0180000041.01.T01:CDS
GAGGAAACATCGCGAACCTG+AGG 0.640455 1:+703122 MsG0180000041.01.T01:CDS
AATGAACAACTTGAAAGGAG+TGG 0.646866 1:+702868 MsG0180000041.01.T01:CDS
CAAAGCAGTAAGAGGAAACG+GGG 0.651217 1:+702955 MsG0180000041.01.T01:CDS
TCAGGCATATATTGAGCCCG+AGG 0.657645 1:-703630 None:intergenic
TCGGGCTCAATATATGCCTG+AGG 0.670652 1:+703632 MsG0180000041.01.T01:CDS
ACTGCTTTGAGAACTATCAG+AGG 0.676720 1:-702941 None:intergenic
TTTCTTGGCCGCTTTCTCAG+CGG 0.699590 1:-702662 None:intergenic
TGAAAGGAGTGGCATTACCG+AGG 0.705152 1:+702879 MsG0180000041.01.T01:CDS
ACTTTCTTGATACTTAGCAG+CGG 0.731548 1:-703234 None:intergenic
GTATGCTTGATATACCCTGG+AGG 0.750443 1:-701579 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTCCAAAAAATCAAAA+TGG - Chr1:703687-703706 None:intergenic 15.0%
!! AAACATAAATTAAAATCACA+TGG - Chr1:701998-702017 None:intergenic 15.0%
!! AACATAAATTAAAATCACAT+GGG - Chr1:701997-702016 None:intergenic 15.0%
!! ACAAATTAGGTTAAAAAATT+GGG - Chr1:701781-701800 None:intergenic 15.0%
!! TACATATTAGATATTAAACA+AGG - Chr1:703058-703077 None:intergenic 15.0%
!!! ATTTATTAATTATTTCTTGG+TGG + Chr1:701961-701980 MsG0180000041.01.T01:intron 15.0%
!!! TTTATTAATTATTTCTTGGT+GGG + Chr1:701962-701981 MsG0180000041.01.T01:intron 15.0%
!!! TTTTAATTCAAAAAAGCTTT+AGG - Chr1:702253-702272 None:intergenic 15.0%
!! ATAAAAACAAAAACCCTAAT+TGG - Chr1:701839-701858 None:intergenic 20.0%
!! CACAAATTAGGTTAAAAAAT+TGG - Chr1:701782-701801 None:intergenic 20.0%
!! TGTAACAAGATAACAAATTA+GGG + Chr1:701893-701912 MsG0180000041.01.T01:intron 20.0%
!! TTAGTATATAGTACATTCAT+CGG - Chr1:703905-703924 None:intergenic 20.0%
!! TTGTAACAAGATAACAAATT+AGG + Chr1:701892-701911 MsG0180000041.01.T01:intron 20.0%
!!! AATTAGGGTTTTTGTTTTTA+TGG + Chr1:701838-701857 MsG0180000041.01.T01:intron 20.0%
!!! ATAACAACATTTTCAAATAG+CGG - Chr1:702566-702585 None:intergenic 20.0%
!!! GTTAAGAACTCTTTTAATAT+TGG + Chr1:702216-702235 MsG0180000041.01.T01:intron 20.0%
!!! TGATTACTTGATTTTATAAC+TGG + Chr1:702487-702506 MsG0180000041.01.T01:intron 20.0%
!!! TTTAACTTATAAAACGTTCT+AGG - Chr1:702027-702046 None:intergenic 20.0%
! ACAATAACCTAACTCTAAAT+TGG - Chr1:702303-702322 None:intergenic 25.0%
! GCTATTTGAAAATGTTGTTA+TGG + Chr1:702565-702584 MsG0180000041.01.T01:intron 25.0%
! TACCATGTAACAGAAAAAAT+TGG - Chr1:702076-702095 None:intergenic 25.0%
! TAGATGATTATAGCTCATTT+AGG + Chr1:702190-702209 MsG0180000041.01.T01:intron 25.0%
! TATGTATTCTTATGTGAAAC+AGG + Chr1:703072-703091 MsG0180000041.01.T01:intron 25.0%
! TATTGTTATGTGAGTTAGTT+AGG + Chr1:702317-702336 MsG0180000041.01.T01:intron 25.0%
! TCTGTAAAAAATTAATGCAC+AGG - Chr1:703766-703785 None:intergenic 25.0%
! TTAGGTTAAAAAATTGGGAT+CGG - Chr1:701776-701795 None:intergenic 25.0%
!! TTTGAAGAAATTTTCTTCTC+TGG - Chr1:701474-701493 None:intergenic 25.0%
!! TTTTTCTGTTGCAATTTTTG+TGG + Chr1:702442-702461 MsG0180000041.01.T01:intron 25.0%
!!! AACCTAATTTGTGATTTTCT+TGG + Chr1:701789-701808 MsG0180000041.01.T01:intron 25.0%
!!! ACAGTTCCATTTTGATTTTT+TGG + Chr1:703678-703697 MsG0180000041.01.T01:exon 25.0%
!!! ACCTAATTTGTGATTTTCTT+GGG + Chr1:701790-701809 MsG0180000041.01.T01:intron 25.0%
!!! TCAAGATTGGTTATTTTGTT+CGG + Chr1:703533-703552 MsG0180000041.01.T01:CDS 25.0%
!!! TGTGCATTAATTTTTTACAG+AGG + Chr1:703765-703784 MsG0180000041.01.T01:three_prime_UTR 25.0%
!!! TTTTTTGTTCAACATGATTG+TGG + Chr1:703835-703854 MsG0180000041.01.T01:three_prime_UTR 25.0%
AAAACAACTATCACTATCGA+TGG - Chr1:702132-702151 None:intergenic 30.0%
AAATTCAATCAAGATCACAC+AGG - Chr1:701677-701696 None:intergenic 30.0%
AATAACCAATCTTGATCCAA+TGG - Chr1:703528-703547 None:intergenic 30.0%
AATCAAAATGGAACTGTGTA+TGG - Chr1:703675-703694 None:intergenic 30.0%
AGAAAAAATTGGTGCATCAT+TGG - Chr1:702065-702084 None:intergenic 30.0%
AGATCTGGATTATACCAATT+AGG + Chr1:701822-701841 MsG0180000041.01.T01:intron 30.0%
ATCCAAACAAGGATTTCAAT+TGG + Chr1:703146-703165 MsG0180000041.01.T01:CDS 30.0%
ATGAGAATGAACAACTTGAA+AGG + Chr1:702863-702882 MsG0180000041.01.T01:CDS 30.0%
CAAGCTCAATAATGAAGTAA+AGG + Chr1:702801-702820 MsG0180000041.01.T01:CDS 30.0%
CAGCCAATAACATATGAATT+GGG + Chr1:703501-703520 MsG0180000041.01.T01:CDS 30.0%
GAAAAAATTGGTGCATCATT+GGG - Chr1:702064-702083 None:intergenic 30.0%
GATCTGGATTATACCAATTA+GGG + Chr1:701823-701842 MsG0180000041.01.T01:intron 30.0%
TATCTGTCACAATGAAATGT+TGG - Chr1:703805-703824 None:intergenic 30.0%
TCAGCCAATAACATATGAAT+TGG + Chr1:703500-703519 MsG0180000041.01.T01:CDS 30.0%
TCCAAACAAGGATTTCAATT+GGG + Chr1:703147-703166 MsG0180000041.01.T01:CDS 30.0%
TCCCAAGAAAATCACAAATT+AGG - Chr1:701794-701813 None:intergenic 30.0%
TTTAAAGATCCGTTTGGATT+TGG + Chr1:702591-702610 MsG0180000041.01.T01:intron 30.0%
! AATCTTCTGTTTCTTTGAGA+CGG - Chr1:703577-703596 None:intergenic 30.0%
! TCTTGAACCAATTTAGAGTT+AGG + Chr1:702293-702312 MsG0180000041.01.T01:intron 30.0%
! TTGGAATTTTTGTAGACAGT+AGG + Chr1:703697-703716 MsG0180000041.01.T01:three_prime_UTR 30.0%
!!! ACATTTTCAAATAGCGGATA+CGG - Chr1:702560-702579 None:intergenic 30.0%
!!! ATAGTTGTTTTGAATGAAGG+TGG + Chr1:702141-702160 MsG0180000041.01.T01:intron 30.0%
!!! CACCAATTTTTTCTGTTACA+TGG + Chr1:702071-702090 MsG0180000041.01.T01:intron 30.0%
!!! GTGATAGTTGTTTTGAATGA+AGG + Chr1:702138-702157 MsG0180000041.01.T01:intron 30.0%
AAAATCTCTATTGCAACCTC+GGG + Chr1:703614-703633 MsG0180000041.01.T01:CDS 35.0%
AACATATGAATTGGGTCCAT+TGG + Chr1:703509-703528 MsG0180000041.01.T01:CDS 35.0%
AATGAACAACTTGAAAGGAG+TGG + Chr1:702868-702887 MsG0180000041.01.T01:CDS 35.0%
ACTTTCTTGATACTTAGCAG+CGG - Chr1:703237-703256 None:intergenic 35.0%
AGAAGAAGGAAAAAAGGAGA+AGG + Chr1:702716-702735 MsG0180000041.01.T01:CDS 35.0%
AGAAGGAGAAGAAGGAAAAA+AGG + Chr1:702710-702729 MsG0180000041.01.T01:CDS 35.0%
ATAGTTCTCAAAGCAGTAAG+AGG + Chr1:702947-702966 MsG0180000041.01.T01:CDS 35.0%
ATCCTTAGAGAGAGAAAGTT+AGG - Chr1:701514-701533 None:intergenic 35.0%
CAAAATCTCTATTGCAACCT+CGG + Chr1:703613-703632 MsG0180000041.01.T01:CDS 35.0%
CACAAAAGCAAAATCGAAAC+TGG - Chr1:701646-701665 None:intergenic 35.0%
CCAAACAAGGATTTCAATTG+GGG + Chr1:703148-703167 MsG0180000041.01.T01:CDS 35.0%
CTTGGGAATTAGAATAGATC+TGG + Chr1:701807-701826 MsG0180000041.01.T01:intron 35.0%
CTTTGTGCGATTCATATCTT+AGG + Chr1:701427-701446 MsG0180000041.01.T01:five_prime_UTR 35.0%
GAAGAAGGAAAAAAGGAGAA+GGG + Chr1:702717-702736 MsG0180000041.01.T01:CDS 35.0%
GCTTGCAATGTTTGATTCTT+TGG + Chr1:702530-702549 MsG0180000041.01.T01:intron 35.0%
GGACCCAATTCATATGTTAT+TGG - Chr1:703507-703526 None:intergenic 35.0%
TCCTTAGAGAGAGAAAGTTA+GGG - Chr1:701513-701532 None:intergenic 35.0%
! GAAGAAATTTTCTTCTCTGG+CGG - Chr1:701471-701490 None:intergenic 35.0%
! TATTGGCTGAATGTGAAGTA+AGG - Chr1:703490-703509 None:intergenic 35.0%
!! ATCTTCTGTTTCTTTGAGAC+GGG - Chr1:703576-703595 None:intergenic 35.0%
!!! TAGAGATTTTGAGCACTGAA+CGG - Chr1:703604-703623 None:intergenic 35.0%
AAGCTTTCTCACCATGATCT+TGG - Chr1:702996-703015 None:intergenic 40.0%
AATGCATATATGTCAGCCTC+AGG - Chr1:703651-703670 None:intergenic 40.0%
ACCCTAACTTTCTCTCTCTA+AGG + Chr1:701509-701528 MsG0180000041.01.T01:five_prime_UTR 40.0%
ACTTCTCATGCAAGTGTTAG+TGG + Chr1:702769-702788 MsG0180000041.01.T01:CDS 40.0%
ATCGTCGATCAAGGAAGAAT+CGG - Chr1:701604-701623 None:intergenic 40.0%
CCCCAATTGAAATCCTTGTT+TGG - Chr1:703151-703170 None:intergenic 40.0%
CTCAAAGCAGTAAGAGGAAA+CGG + Chr1:702953-702972 MsG0180000041.01.T01:CDS 40.0%
CTTAATCGAATCGTCGATCA+AGG - Chr1:701613-701632 None:intergenic 40.0%
CTTAATGTGCGATTGATGTG+TGG + Chr1:702340-702359 MsG0180000041.01.T01:intron 40.0%
CTTGATCGACGATTCGATTA+AGG + Chr1:701611-701630 MsG0180000041.01.T01:CDS 40.0%
GCTTTCTTAATACTGGGCAA+TGG - Chr1:703327-703346 None:intergenic 40.0%
GGCAATGGTTTACAGACTTT+GGG - Chr1:703384-703403 None:intergenic 40.0%
GTCTCGGCTTTCTTAATACT+GGG - Chr1:703333-703352 None:intergenic 40.0%
TCAAAGCAGTAAGAGGAAAC+GGG + Chr1:702954-702973 MsG0180000041.01.T01:CDS 40.0%
TGAGAACTATCAGAGGAGTA+GGG - Chr1:702937-702956 None:intergenic 40.0%
TTGAGAACTATCAGAGGAGT+AGG - Chr1:702938-702957 None:intergenic 40.0%
! AATCCTTGTTTGGATTCCTC+AGG - Chr1:703141-703160 None:intergenic 40.0%
! AATTTCCATGGAGCTGACTT+GGG - Chr1:703356-703375 None:intergenic 40.0%
! AGTAGGTGTAGCTTTTAGCA+GGG + Chr1:703714-703733 MsG0180000041.01.T01:three_prime_UTR 40.0%
! TGATGATGAAAGTCGAAGGA+TGG + Chr1:703449-703468 MsG0180000041.01.T01:CDS 40.0%
!! ACTGCTTTGAGAACTATCAG+AGG - Chr1:702944-702963 None:intergenic 40.0%
!! CAGTTGATGATGAAAGTCGA+AGG + Chr1:703445-703464 MsG0180000041.01.T01:CDS 40.0%
!! GGGATCGGAAACTTGTTTTT+GGG - Chr1:701761-701780 None:intergenic 40.0%
!! TGGGATCGGAAACTTGTTTT+TGG - Chr1:701762-701781 None:intergenic 40.0%
!! TGGTGCTTTAAAGATCCGTT+TGG + Chr1:702585-702604 MsG0180000041.01.T01:intron 40.0%
AAAAGGGAAGCAACGAGACA+TGG - Chr1:701556-701575 None:intergenic 45.0%
AACAGGTTGCTCAAGTTCCT+CGG - Chr1:702899-702918 None:intergenic 45.0%
AAGAGAGGAGAAGGACAAGA+AGG + Chr1:702630-702649 MsG0180000041.01.T01:CDS 45.0%
ATATACCCTGGAGGTGGAAA+AGG - Chr1:701573-701592 None:intergenic 45.0%
CAAAGCAGTAAGAGGAAACG+GGG + Chr1:702955-702974 MsG0180000041.01.T01:CDS 45.0%
CATTCATCGGATACAACAGC+TGG - Chr1:703892-703911 None:intergenic 45.0%
CCAGAAAGAAAGAGAGGAGA+AGG + Chr1:702621-702640 MsG0180000041.01.T01:CDS 45.0%
CCTTCTCCTCTCTTTCTTTC+TGG - Chr1:702624-702643 None:intergenic 45.0%
CGGCAATGGTTTACAGACTT+TGG - Chr1:703385-703404 None:intergenic 45.0%
CTGTAAACCATTGCCGAATG+TGG + Chr1:703389-703408 MsG0180000041.01.T01:CDS 45.0%
GAACCTGAGGAATCCAAACA+AGG + Chr1:703135-703154 MsG0180000041.01.T01:CDS 45.0%
GAAGCTCCAGAAAGAAAGAG+AGG + Chr1:702615-702634 MsG0180000041.01.T01:CDS 45.0%
GAATTTCCATGGAGCTGACT+TGG - Chr1:703357-703376 None:intergenic 45.0%
GAGTTACCTAGCCAAGATCA+TGG + Chr1:702982-703001 MsG0180000041.01.T01:CDS 45.0%
GATGTTTCCTCAATGGAAGC+CGG - Chr1:703113-703132 None:intergenic 45.0%
GCAACACCAACACTTCTTGA+CGG - Chr1:703267-703286 None:intergenic 45.0%
GCATCAACAGGAACCACATT+CGG - Chr1:703405-703424 None:intergenic 45.0%
GGAGCTTCTCCAAATCCAAA+CGG - Chr1:702603-702622 None:intergenic 45.0%
GGTCTCGGCTTTCTTAATAC+TGG - Chr1:703334-703353 None:intergenic 45.0%
GTATGCTTGATATACCCTGG+AGG - Chr1:701582-701601 None:intergenic 45.0%
TATACCCTGGAGGTGGAAAA+GGG - Chr1:701572-701591 None:intergenic 45.0%
TCGGTATGCTTGATATACCC+TGG - Chr1:701585-701604 None:intergenic 45.0%
TGGGTCCATTGGATCAAGAT+TGG + Chr1:703520-703539 MsG0180000041.01.T01:CDS 45.0%
TTCTCACCATGATCTTGGCT+AGG - Chr1:702991-703010 None:intergenic 45.0%
! AGTTGCAGATTTGCTTGCTC+GGG + Chr1:703191-703210 MsG0180000041.01.T01:CDS 45.0%
! CAGTAGGTGTAGCTTTTAGC+AGG + Chr1:703713-703732 MsG0180000041.01.T01:three_prime_UTR 45.0%
! CTTTGGGAGCAGAATTTCCA+TGG - Chr1:703368-703387 None:intergenic 45.0%
! GAGTTGCAGATTTGCTTGCT+CGG + Chr1:703190-703209 MsG0180000041.01.T01:CDS 45.0%
! GTAGGTGTAGCTTTTAGCAG+GGG + Chr1:703715-703734 MsG0180000041.01.T01:three_prime_UTR 45.0%
! GTGTTGCAGATTTGCTTGCT+CGG + Chr1:703280-703299 MsG0180000041.01.T01:CDS 45.0%
! TGTTGCAGATTTGCTTGCTC+GGG + Chr1:703281-703300 MsG0180000041.01.T01:CDS 45.0%
!! ATTTCCATGGAGCTGACTTG+GGG - Chr1:703355-703374 None:intergenic 45.0%
!! GAGACGGGCATTGTTTGTTT+TGG - Chr1:703561-703580 None:intergenic 45.0%
!!! AATATTTATTAATTATTTCT+TGG + Chr1:701958-701977 MsG0180000041.01.T01:intron 5.0%
ACAGGAACCACATTCGGCAA+TGG - Chr1:703399-703418 None:intergenic 50.0%
AGAAAGAGAGGAAGGAGAGG+AGG + Chr1:702680-702699 MsG0180000041.01.T01:CDS 50.0%
AGAAAGCGGCCAAGAAAGAG+AGG + Chr1:702668-702687 MsG0180000041.01.T01:CDS 50.0%
AGACAGTGCGAAGTTGCGTA+AGG + Chr1:702831-702850 MsG0180000041.01.T01:CDS 50.0%
AGGAGAAGGACAAGAAGGAC+AGG + Chr1:702635-702654 MsG0180000041.01.T01:CDS 50.0%
CAATGGAAGCCGGATTCGAA+TGG - Chr1:703103-703122 None:intergenic 50.0%
CCAAGAAAGAGAGGAAGGAG+AGG + Chr1:702677-702696 MsG0180000041.01.T01:CDS 50.0%
CCTCTCCTTCCTCTCTTTCT+TGG - Chr1:702680-702699 None:intergenic 50.0%
GAGGGAGAAGAAGGAGAAGA+AGG + Chr1:702702-702721 MsG0180000041.01.T01:CDS 50.0%
GGCTCTTGAGGAGAACAAAC+AGG - Chr1:702916-702935 None:intergenic 50.0%
GGTTCGCGATGTTTCCTCAA+TGG - Chr1:703120-703139 None:intergenic 50.0%
TCAGGCATATATTGAGCCCG+AGG - Chr1:703633-703652 None:intergenic 50.0%
TCGGGCTCAATATATGCCTG+AGG + Chr1:703632-703651 MsG0180000041.01.T01:CDS 50.0%
TGAAAGGAGTGGCATTACCG+AGG + Chr1:702879-702898 MsG0180000041.01.T01:CDS 50.0%
TGCTTGATATACCCTGGAGG+TGG - Chr1:701579-701598 None:intergenic 50.0%
TTCGAATCCGGCTTCCATTG+AGG + Chr1:703103-703122 MsG0180000041.01.T01:CDS 50.0%
! GAGACACCGTCAAGAAGTGT+TGG + Chr1:703258-703277 MsG0180000041.01.T01:CDS 50.0%
! GGGTCATGTTCGAGAAGTGT+AGG + Chr1:703168-703187 MsG0180000041.01.T01:CDS 50.0%
! TTTCTTGGCCGCTTTCTCAG+CGG - Chr1:702665-702684 None:intergenic 50.0%
AAGAGAGGAAGGAGAGGAGG+AGG + Chr1:702683-702702 MsG0180000041.01.T01:CDS 55.0%
AGAGAGGAAGGAGAGGAGGA+GGG + Chr1:702684-702703 MsG0180000041.01.T01:CDS 55.0%
AGCGGCCAAGAAAGAGAGGA+AGG + Chr1:702672-702691 MsG0180000041.01.T01:CDS 55.0%
CAGGAAAGCCGCTGAGAAAG+CGG + Chr1:702654-702673 MsG0180000041.01.T01:CDS 55.0%
CAGGACCTGCCATTCGAATC+CGG + Chr1:703091-703110 MsG0180000041.01.T01:intron 55.0%
CGGATACAACAGCTGGTGTC+TGG - Chr1:703885-703904 None:intergenic 55.0%
GAGGAAACATCGCGAACCTG+AGG + Chr1:703122-703141 MsG0180000041.01.T01:CDS 55.0%
GCTGCTGCTACAGCATCAAC+AGG - Chr1:703417-703436 None:intergenic 55.0%
TCAGAGGAGTAGGGCTCTTG+AGG - Chr1:702928-702947 None:intergenic 55.0%
! GCTTCCCTTTTCCACCTCCA+GGG + Chr1:701565-701584 MsG0180000041.01.T01:CDS 55.0%
! TGCTTCCCTTTTCCACCTCC+AGG + Chr1:701564-701583 MsG0180000041.01.T01:CDS 55.0%
!! ATGGAGCTGACTTGGGGTCT+CGG - Chr1:703349-703368 None:intergenic 55.0%
GAGACCCCAAGTCAGCTCCA+TGG + Chr1:703348-703367 MsG0180000041.01.T01:CDS 60.0%
GGAAGCCGGATTCGAATGGC+AGG - Chr1:703099-703118 None:intergenic 60.0%
GGAGAGGAGGAGGGAGAAGA+AGG + Chr1:702693-702712 MsG0180000041.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 701420 703954 701420 ID=MsG0180000041.01;Name=MsG0180000041.01
Chr1 mRNA 701420 703954 701420 ID=MsG0180000041.01.T01;Parent=MsG0180000041.01;Name=MsG0180000041.01.T01;_AED=0.45;_eAED=0.45;_QI=135|1|1|1|0.5|0.33|3|262|360
Chr1 exon 703094 703954 703094 ID=MsG0180000041.01.T01:exon:7742;Parent=MsG0180000041.01.T01
Chr1 exon 702598 703003 702598 ID=MsG0180000041.01.T01:exon:7741;Parent=MsG0180000041.01.T01
Chr1 exon 701420 701632 701420 ID=MsG0180000041.01.T01:exon:7740;Parent=MsG0180000041.01.T01
Chr1 five_prime_UTR 701420 701554 701420 ID=MsG0180000041.01.T01:five_prime_utr;Parent=MsG0180000041.01.T01
Chr1 CDS 701555 701632 701555 ID=MsG0180000041.01.T01:cds;Parent=MsG0180000041.01.T01
Chr1 CDS 702598 703003 702598 ID=MsG0180000041.01.T01:cds;Parent=MsG0180000041.01.T01
Chr1 CDS 703094 703692 703094 ID=MsG0180000041.01.T01:cds;Parent=MsG0180000041.01.T01
Chr1 three_prime_UTR 703693 703954 703693 ID=MsG0180000041.01.T01:three_prime_utr;Parent=MsG0180000041.01.T01
Gene Sequence

>MsG0180000041.01.T01

ATGTCTCGTTGCTTCCCTTTTCCACCTCCAGGGTATATCAAGCATACCGATTCTTCCTTGATCGACGATTCGATTAAGATCCGTTTGGATTTGGAGAAGCTCCAGAAAGAAAGAGAGGAGAAGGACAAGAAGGACAGGAAAGCCGCTGAGAAAGCGGCCAAGAAAGAGAGGAAGGAGAGGAGGAGGGAGAAGAAGGAGAAGAAGGAAAAAAGGAGAAGGGAAAAAGAAGAGAAACTAAAAGATTCAACAACTTCTCATGCAAGTGTTAGTGGTATAAAGTTCAAGCTCAATAATGAAGTAAAGGAGCACAAAGACAGTGCGAAGTTGCGTAAGGTTGAAGATTATGAGAATGAACAACTTGAAAGGAGTGGCATTACCGAGGAACTTGAGCAACCTGTTTGTTCTCCTCAAGAGCCCTACTCCTCTGATAGTTCTCAAAGCAGTAAGAGGAAACGGGGCATTGAGTTACCTAGCCAAGATCATGGACCTGCCATTCGAATCCGGCTTCCATTGAGGAAACATCGCGAACCTGAGGAATCCAAACAAGGATTTCAATTGGGGTCATGTTCGAGAAGTGTAGGAGTTGCAGATTTGCTTGCTCGGGATACTAGCAGAATTGAACGTCCGCTGCTAAGTATCAAGAAAGTCGAGACACCGTCAAGAAGTGTTGGTGTTGCAGATTTGCTTGCTCGGGATACTAGCAGAACTGAACGTCCATTGCCCAGTATTAAGAAAGCCGAGACCCCAAGTCAGCTCCATGGAAATTCTGCTCCCAAAGTCTGTAAACCATTGCCGAATGTGGTTCCTGTTGATGCTGTAGCAGCAGCAAATGAGACAGTTGATGATGAAAGTCGAAGGATGGAATCATTGTATAAATCCTTACTTCACATTCAGCCAATAACATATGAATTGGGTCCATTGGATCAAGATTGGTTATTTTGTTCGGTACCAAAACAAACAATGCCCGTCTCAAAGAAACAGAAGATTGATGCCGTTCAGTGCTCAAAATCTCTATTGCAACCTCGGGCTCAATATATGCCTGAGGCTGACATATATGCATTACCATACACAGTTCCATTTTGA
Protein sequence

>MsG0180000041.01.T01

MSRCFPFPPPGYIKHTDSSLIDDSIKIRLDLEKLQKEREEKDKKDRKAAEKAAKKERKERRREKKEKKEKRRREKEEKLKDSTTSHASVSGIKFKLNNEVKEHKDSAKLRKVEDYENEQLERSGITEELEQPVCSPQEPYSSDSSQSSKRKRGIELPSQDHGPAIRIRLPLRKHREPEESKQGFQLGSCSRSVGVADLLARDTSRIERPLLSIKKVETPSRSVGVADLLARDTSRTERPLPSIKKAETPSQLHGNSAPKVCKPLPNVVPVDAVAAANETVDDESRRMESLYKSLLHIQPITYELGPLDQDWLFCSVPKQTMPVSKKQKIDAVQCSKSLLQPRAQYMPEADIYALPYTVPF*