AlfalfaGEDB | MsG0580024204.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024204.01

KEGG: -
GO: GO:0005622 GO:0006886
PFAM: PF03127

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024204.01.T01	XP_003610830.1	100	144	0	0	23	166	256	399	3.19E-96	294

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024204.01.T01	Q9LFL3	59.603	151	51	4	23	166	260	407	1.00E-36	133

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024204.01.T01	G7K569	100.000	144	0	0	23	166	256	399	1.52e-96	294

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048177.01	MsG0580024204.01	0.823772	1.157185e-53	1.005933e-50
MsG0080048915.01	MsG0580024204.01	0.800636	1.313128e-48	6.145108e-46
MsG0180000041.01	MsG0580024204.01	0.842978	1.857856e-58	2.864670e-55
MsG0180000222.01	MsG0580024204.01	0.818875	1.559738e-52	1.181743e-49
MsG0180000235.01	MsG0580024204.01	0.801408	9.134128e-49	4.358713e-46
MsG0180000567.01	MsG0580024204.01	0.812938	3.296123e-51	2.123417e-48
MsG0180000645.01	MsG0580024204.01	0.812036	5.188195e-51	3.262876e-48
MsG0180000822.01	MsG0580024204.01	0.800817	1.206521e-48	5.671931e-46
MsG0180001043.01	MsG0580024204.01	0.834185	3.468490e-56	4.084565e-53
MsG0180001355.01	MsG0580024204.01	0.818523	1.875420e-52	1.407143e-49
MsG0180001921.01	MsG0580024204.01	0.854093	1.548120e-61	3.419069e-58
MsG0180002912.01	MsG0580024204.01	0.868845	4.823860e-66	1.771036e-62
MsG0180003241.01	MsG0580024204.01	0.802731	4.880817e-49	2.408871e-46
MsG0180003948.01	MsG0580024204.01	0.812699	3.718196e-51	2.380413e-48
MsG0180004005.01	MsG0580024204.01	0.806637	7.468375e-50	4.074010e-47
MsG0180004060.01	MsG0580024204.01	0.840527	8.243671e-58	1.177514e-54
MsG0180004162.01	MsG0580024204.01	0.832643	8.407128e-56	9.454437e-53
MsG0180004258.01	MsG0580024204.01	0.814692	1.353872e-51	9.146671e-49
MsG0180004388.01	MsG0580024204.01	0.843765	1.145412e-58	1.810518e-55
MsG0180004762.01	MsG0580024204.01	0.842791	2.084154e-58	3.194772e-55
MsG0180005125.01	MsG0580024204.01	0.862030	6.760727e-64	1.951821e-60
MsG0180005172.01	MsG0580024204.01	0.846457	2.146084e-59	3.696572e-56
MsG0180005813.01	MsG0580024204.01	0.804491	2.106182e-49	1.087384e-46
MsG0180005878.01	MsG0580024204.01	0.800935	1.141194e-48	5.381304e-46
MsG0180005944.01	MsG0580024204.01	0.842491	2.503391e-58	3.802119e-55
MsG0180006012.01	MsG0580024204.01	0.814410	1.562578e-51	1.047749e-48
MsG0180006016.01	MsG0580024204.01	0.862540	4.713341e-64	1.385218e-60
MsG0180006047.01	MsG0580024204.01	0.832874	7.369187e-56	8.344286e-53
MsG0180006226.01	MsG0580024204.01	-0.805168	1.520335e-49	7.987873e-47
MsG0280010614.01	MsG0580024204.01	0.831515	1.598057e-55	1.738173e-52
MsG0280010739.01	MsG0580024204.01	0.814049	1.877636e-51	1.246639e-48
MsG0280010784.01	MsG0580024204.01	0.804117	2.520019e-49	1.288657e-46
MsG0280011024.01	MsG0580024204.01	0.800232	1.587646e-48	7.353861e-46
MsG0280011141.01	MsG0580024204.01	0.808168	3.535427e-50	2.007298e-47
MsG0280011152.01	MsG0580024204.01	0.804008	2.654188e-49	1.353511e-46
MsG0280011235.01	MsG0580024204.01	0.828908	6.921317e-55	6.973503e-52
MsG0280011446.01	MsG0580024204.01	0.838290	3.140716e-57	4.187167e-54
MsG0380011754.01	MsG0580024204.01	0.855029	8.297364e-62	1.889682e-58
MsG0380012033.01	MsG0580024204.01	0.800372	1.486703e-48	6.911127e-46
MsG0380013047.01	MsG0580024204.01	0.844247	8.508589e-59	1.365379e-55
MsG0380014126.01	MsG0580024204.01	0.849964	2.306082e-60	4.447686e-57
MsG0380014163.01	MsG0580024204.01	0.835528	1.592539e-56	1.952585e-53
MsG0380014680.01	MsG0580024204.01	0.897854	9.383014e-77	1.080334e-72
MsG0480018640.01	MsG0580024204.01	0.817910	2.580400e-52	1.903621e-49
MsG0480018904.01	MsG0580024204.01	0.855004	8.438535e-62	1.920257e-58
MsG0480018970.01	MsG0580024204.01	0.865884	4.271389e-65	1.411781e-61
MsG0480019816.01	MsG0580024204.01	0.814636	1.393035e-51	9.397230e-49
MsG0480020219.01	MsG0580024204.01	0.808070	3.709905e-50	2.100738e-47
MsG0480020445.01	MsG0580024204.01	0.842911	1.936508e-58	2.979667e-55
MsG0480020720.01	MsG0580024204.01	0.807682	4.485962e-50	2.514460e-47
MsG0480020886.01	MsG0580024204.01	0.815566	8.655880e-52	5.988910e-49
MsG0480021240.01	MsG0580024204.01	0.810973	8.830323e-51	5.399949e-48
MsG0480021642.01	MsG0580024204.01	0.802285	6.031113e-49	2.942998e-46
MsG0480021953.01	MsG0580024204.01	0.823260	1.523967e-53	1.305591e-50
MsG0480022274.01	MsG0580024204.01	0.848876	4.634132e-60	8.626582e-57
MsG0480022301.01	MsG0580024204.01	0.802389	5.742879e-49	2.809762e-46
MsG0480022480.01	MsG0580024204.01	0.873559	1.338380e-67	5.834168e-64
MsG0480022486.01	MsG0580024204.01	0.813490	2.493169e-51	1.630524e-48
MsG0480022520.01	MsG0580024204.01	0.809603	1.744054e-50	1.028536e-47
MsG0480022661.01	MsG0580024204.01	0.812709	3.699367e-51	2.368918e-48
MsG0480023088.01	MsG0580024204.01	0.832485	9.204493e-56	1.030231e-52
MsG0480023224.01	MsG0580024204.01	0.837106	6.322477e-57	8.129325e-54
MsG0480023245.01	MsG0580024204.01	0.863202	2.946491e-64	8.859535e-61
MsG0480023280.01	MsG0580024204.01	0.804759	1.851140e-49	9.624472e-47
MsG0480023318.01	MsG0580024204.01	0.819932	8.955509e-53	6.987588e-50
MsG0480023412.01	MsG0580024204.01	0.831252	1.855073e-55	2.001790e-52
MsG0480023417.01	MsG0580024204.01	0.824063	9.885617e-54	8.663214e-51
MsG0480023517.01	MsG0580024204.01	0.864536	1.132792e-64	3.568827e-61
MsG0480023554.01	MsG0580024204.01	0.832208	1.077981e-55	1.196607e-52
MsG0480023581.01	MsG0580024204.01	0.802070	6.678950e-49	3.241171e-46
MsG0480023947.01	MsG0580024204.01	0.850281	1.879578e-60	3.662619e-57
MsG0580024056.01	MsG0580024204.01	0.826554	2.545592e-54	2.395088e-51
MsG0580024073.01	MsG0580024204.01	0.820349	7.188758e-53	5.674155e-50
MsG0580024204.01	MsG0580024205.01	0.856892	2.366193e-62	5.733075e-59
MsG0580024204.01	MsG0580024393.01	0.808375	3.194588e-50	1.823615e-47
MsG0580024204.01	MsG0580024407.01	0.853018	3.153591e-61	6.717504e-58
MsG0580024204.01	MsG0580024682.01	0.825337	4.952063e-54	4.500914e-51
MsG0580024204.01	MsG0580024733.01	0.812920	3.325006e-51	2.141040e-48
MsG0580024204.01	MsG0580025046.01	0.838530	2.722622e-57	3.656898e-54
MsG0580024204.01	MsG0580025134.01	0.827393	1.604081e-54	1.546755e-51
MsG0580024204.01	MsG0580025143.01	0.807882	4.067181e-50	2.291620e-47
MsG0580024204.01	MsG0580025328.01	0.800945	1.135946e-48	5.357927e-46
MsG0580024204.01	MsG0580025989.01	0.832049	1.179914e-55	1.303819e-52
MsG0580024204.01	MsG0580027129.01	0.824286	8.763373e-54	7.728026e-51
MsG0580024204.01	MsG0580027345.01	0.842266	2.872668e-58	4.332118e-55
MsG0580024204.01	MsG0580027441.01	0.809661	1.694559e-50	1.000894e-47
MsG0580024204.01	MsG0580027532.01	0.821221	4.530031e-53	3.663543e-50
MsG0580024204.01	MsG0580027847.01	0.824925	6.198602e-54	5.568369e-51
MsG0580024204.01	MsG0580028185.01	0.808963	2.391948e-50	1.386622e-47
MsG0580024204.01	MsG0580028355.01	0.836476	9.153500e-57	1.154845e-53
MsG0580024204.01	MsG0580028416.01	0.809967	1.456166e-50	8.670122e-48
MsG0580024204.01	MsG0580028969.01	0.811038	8.548112e-51	5.236018e-48
MsG0580024204.01	MsG0580029155.01	0.814637	1.392141e-51	9.391664e-49
MsG0580024204.01	MsG0580029189.01	0.806893	6.594092e-50	3.621108e-47
MsG0580024204.01	MsG0580029594.01	0.820001	8.639480e-53	6.753709e-50
MsG0580024204.01	MsG0580029629.01	0.808522	2.971479e-50	1.702800e-47
MsG0580024204.01	MsG0580029672.01	0.806493	8.008030e-50	4.352641e-47
MsG0580024204.01	MsG0580029758.01	0.850297	1.860027e-60	3.626663e-57
MsG0580024204.01	MsG0580029762.01	0.812161	4.873363e-51	3.075586e-48
MsG0580024204.01	MsG0580029767.01	0.816061	6.713321e-52	4.708367e-49
MsG0580024204.01	MsG0580029798.01	0.800442	1.438797e-48	6.700043e-46
MsG0580024204.01	MsG0580029906.01	0.837161	6.119536e-57	7.881537e-54
MsG0580024204.01	MsG0580030235.01	0.856741	2.621485e-62	6.318362e-59
MsG0580024204.01	MsG0680030685.01	0.838956	2.113231e-57	2.875347e-54
MsG0580024204.01	MsG0680031538.01	0.862647	4.368865e-64	1.288737e-60
MsG0580024204.01	MsG0680031651.01	0.807186	5.716218e-50	3.163046e-47
MsG0580024204.01	MsG0680031719.01	0.831163	1.949974e-55	2.098816e-52
MsG0580024204.01	MsG0680033339.01	0.806214	9.171857e-50	4.950070e-47
MsG0580024204.01	MsG0680034450.01	0.810137	1.338371e-50	8.004023e-48
MsG0580024204.01	MsG0680035582.01	0.800691	1.279860e-48	5.997595e-46
MsG0580024204.01	MsG0780037109.01	0.817079	3.971565e-52	2.863964e-49
MsG0580024204.01	MsG0780037258.01	0.806434	8.242781e-50	4.473412e-47
MsG0580024204.01	MsG0780037895.01	0.804811	1.806066e-49	9.402772e-47
MsG0580024204.01	MsG0780038444.01	0.836101	1.140086e-56	1.422310e-53
MsG0580024204.01	MsG0780038495.01	0.828185	1.034687e-54	1.020896e-51
MsG0580024204.01	MsG0780039900.01	0.807250	5.539900e-50	3.070644e-47
MsG0580024204.01	MsG0780040144.01	0.814569	1.441338e-51	9.705543e-49
MsG0580024204.01	MsG0780040360.01	0.802190	6.310996e-49	3.071819e-46
MsG0580024204.01	MsG0780040442.01	0.841786	3.847381e-58	5.714387e-55
MsG0580024204.01	MsG0780040505.01	0.882365	1.107026e-70	6.724210e-67
MsG0580024204.01	MsG0780040516.01	0.818017	2.440613e-52	1.805811e-49
MsG0580024204.01	MsG0780040601.01	0.817417	3.334509e-52	2.427008e-49
MsG0580024204.01	MsG0780040679.01	0.842049	3.278060e-58	4.909414e-55
MsG0580024204.01	MsG0780040861.01	0.822181	2.717525e-53	2.257807e-50
MsG0580024204.01	MsG0780041161.01	0.828000	1.146562e-54	1.125211e-51
MsG0580024204.01	MsG0780041357.01	0.800174	1.631286e-48	7.544746e-46
MsG0580024204.01	MsG0780041399.01	0.814944	1.190092e-51	8.094904e-49
MsG0580024204.01	MsG0780041429.01	0.803756	2.994109e-49	1.516989e-46
MsG0580024204.01	MsG0780041469.01	0.819098	1.388176e-52	1.058213e-49
MsG0580024204.01	MsG0780041485.01	0.829302	5.554227e-55	5.663124e-52
MsG0580024204.01	MsG0880041985.01	0.872345	3.416178e-67	1.424513e-63
MsG0580024204.01	MsG0880042543.01	0.812369	4.390066e-51	2.786095e-48
MsG0580024204.01	MsG0880042589.01	0.812236	4.691822e-51	2.967092e-48
MsG0580024204.01	MsG0880042811.01	0.809466	1.866554e-50	1.096699e-47
MsG0580024204.01	MsG0880042843.01	0.858128	1.019049e-62	2.573832e-59
MsG0580024204.01	MsG0880042957.01	0.876754	1.085580e-68	5.324920e-65
MsG0580024204.01	MsG0880042967.01	0.866246	3.281274e-65	1.098483e-61
MsG0580024204.01	MsG0880043001.01	0.809812	1.572595e-50	9.325283e-48
MsG0580024204.01	MsG0880043002.01	0.805895	1.070077e-49	5.728270e-47
MsG0580024204.01	MsG0880043082.01	0.817582	3.060976e-52	2.237902e-49
MsG0580024204.01	MsG0880043745.01	0.819248	1.283299e-52	9.823141e-50
MsG0580024204.01	MsG0880045099.01	0.848548	5.714882e-60	1.052474e-56
MsG0580024204.01	MsG0880045106.01	0.848302	6.683619e-60	1.221213e-56
MsG0580024204.01	MsG0880045518.01	0.841608	4.285617e-58	6.331135e-55
MsG0580024204.01	MsG0880046058.01	0.808213	3.458294e-50	1.965799e-47
MsG0580024204.01	MsG0880046113.01	0.813331	2.702169e-51	1.759486e-48
MsG0580024204.01	MsG0880046209.01	0.829300	5.562052e-55	5.670677e-52
MsG0580024204.01	MsG0880046244.01	0.821954	3.067028e-53	2.531811e-50
MsG0580024204.01	MsG0880046329.01	0.843322	1.504463e-58	2.345563e-55
MsG0580024204.01	MsG0880046467.01	0.814166	1.768999e-51	1.178244e-48
MsG0580024204.01	MsG0880046586.01	0.825929	3.584738e-54	3.313596e-51
MsG0580024204.01	MsG0880046642.01	0.849748	2.649552e-60	5.073739e-57
MsG0580024204.01	MsG0880046731.01	0.811906	5.538185e-51	3.470782e-48
MsG0580024204.01	MsG0880046733.01	0.820527	6.542586e-53	5.189419e-50
MsG0580024204.01	MsG0880046753.01	0.815566	8.655493e-52	5.988715e-49
MsG0580024204.01	MsG0880046779.01	0.800415	1.456700e-48	6.779095e-46
MsG0580024204.01	MsG0880047019.01	0.802088	6.623423e-49	3.215659e-46
MsG0580024204.01	MsG0880047143.01	0.836495	9.049967e-57	1.142387e-53
MsG0580024204.01	MsG0880047497.01	0.834183	3.473536e-56	4.090175e-53
MsG0580024204.01	MsG0880047590.01	0.843591	1.275664e-58	2.005646e-55
MsG0380015181.01	MsG0580024204.01	0.834486	2.915267e-56	3.464732e-53
MsG0380015324.01	MsG0580024204.01	0.820318	7.306537e-53	5.762048e-50
MsG0380015779.01	MsG0580024204.01	0.839418	1.603621e-57	2.213269e-54
MsG0380015931.01	MsG0580024204.01	0.829548	4.841304e-55	4.970477e-52
MsG0380016227.01	MsG0580024204.01	0.822228	2.650718e-53	2.205350e-50
MsG0380016264.01	MsG0580024204.01	0.806001	1.016846e-49	5.458214e-47
MsG0380016273.01	MsG0580024204.01	0.806563	7.743025e-50	4.216082e-47
MsG0380016859.01	MsG0580024204.01	0.829729	4.373645e-55	4.514983e-52
MsG0380016971.01	MsG0580024204.01	0.811500	6.788505e-51	4.209134e-48
MsG0380017146.01	MsG0580024204.01	0.852154	5.562092e-61	1.151771e-57
MsG0380017158.01	MsG0580024204.01	0.814347	1.613368e-51	1.079909e-48
MsG0380017285.01	MsG0580024204.01	0.808374	3.196565e-50	1.824685e-47
MsG0380017286.01	MsG0580024204.01	0.834685	2.598465e-56	3.106106e-53
MsG0380017403.01	MsG0580024204.01	0.835408	1.707680e-56	2.086405e-53
MsG0380017604.01	MsG0580024204.01	0.823139	1.626295e-53	1.388641e-50
MsG0380017631.01	MsG0580024204.01	0.828177	1.039034e-54	1.024968e-51
MsG0380017940.01	MsG0580024204.01	0.814843	1.253191e-51	8.501064e-49
MsG0380018009.01	MsG0580024204.01	0.837258	5.779767e-57	7.465548e-54
MsG0380018016.01	MsG0580024204.01	0.800324	1.520033e-48	7.057552e-46
MsG0480018126.01	MsG0580024204.01	0.824566	7.528713e-54	6.693834e-51
MsG0480018160.01	MsG0580024204.01	0.800764	1.236680e-48	5.805978e-46
MsG0280006586.01	MsG0580024204.01	0.825702	4.059739e-54	3.728360e-51
MsG0280006595.01	MsG0580024204.01	0.831846	1.324127e-55	1.454588e-52
MsG0280006953.01	MsG0580024204.01	0.809225	2.102042e-50	1.227218e-47
MsG0280007089.01	MsG0580024204.01	0.804910	1.721645e-49	8.986563e-47
MsG0280007133.01	MsG0580024204.01	-0.816323	5.866592e-52	4.144192e-49
MsG0280007253.01	MsG0580024204.01	0.816765	4.670547e-52	3.339271e-49
MsG0280007481.01	MsG0580024204.01	0.823627	1.251095e-53	1.083053e-50
MsG0280007549.01	MsG0580024204.01	0.802332	5.899329e-49	2.882100e-46
MsG0280007584.01	MsG0580024204.01	0.817382	3.395269e-52	2.468932e-49
MsG0280007585.01	MsG0580024204.01	0.825996	3.456207e-54	3.200880e-51
MsG0280007609.01	MsG0580024204.01	0.820997	5.102123e-53	4.100743e-50
MsG0280007610.01	MsG0580024204.01	0.800900	1.159855e-48	5.464661e-46
MsG0280007636.01	MsG0580024204.01	0.808958	2.397894e-50	1.389884e-47
MsG0280007648.01	MsG0580024204.01	0.816258	6.067818e-52	4.278603e-49
MsG0280007815.01	MsG0580024204.01	0.803054	4.186812e-49	2.083235e-46
MsG0280008220.01	MsG0580024204.01	0.807661	4.532393e-50	2.539093e-47
MsG0280008286.01	MsG0580024204.01	0.806124	9.578214e-50	5.157401e-47
MsG0280009235.01	MsG0580024204.01	0.876816	1.033163e-68	5.079720e-65
MsG0280010275.01	MsG0580024204.01	-0.802124	6.509848e-49	3.163357e-46
MsG0280010276.01	MsG0580024204.01	-0.800557	1.362660e-48	6.364148e-46
MsG0280010416.01	MsG0580024204.01	0.830545	2.764787e-55	2.921880e-52
MsG0280010459.01	MsG0580024204.01	0.810566	1.081382e-50	6.540654e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024204.01.T01	MTR_5g007480	100.000	144	0	0	23	166	256	399	3.86e-100	294
MsG0580024204.01.T01	MTR_4g077940	61.538	143	49	3	23	163	269	407	8.92e-40	139
MsG0580024204.01.T01	MTR_4g077940	61.538	143	49	3	23	163	296	434	1.02e-39	139

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024204.01.T01	AT5G16880	59.603	151	51	4	23	166	260	407	1.02e-37	133
MsG0580024204.01.T01	AT5G16880	59.603	151	51	4	23	166	260	407	1.02e-37	133
MsG0580024204.01.T01	AT5G16880	59.603	151	51	4	23	166	260	407	1.02e-37	133
MsG0580024204.01.T01	AT1G06210	48.077	52	27	0	22	73	254	305	6.88e-12	62.8

Find 41 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAATTTCATCATTAACATTC+AGG	0.240701	5:+1994184	None:intergenic
TATTAGTAGTGCAGCTTAGA+AGG	0.297112	5:+1993867	None:intergenic
TGGAAATAAAGATCACATTT+AGG	0.325504	5:+1994318	None:intergenic
GCCAGTCAATGTTCCATGTT+AGG	0.334938	5:-1994375	None:intergenic
TGATCTTTATTTCCATCTAC+AGG	0.400129	5:-1994310	MsG0580024204.01.T01:CDS
TGGATCAAGACCTGGTGTCC+AGG	0.433352	5:-1994010	MsG0580024204.01.T01:CDS
ACAGGTATCATAGCTGGTTC+AGG	0.438254	5:+1994105	None:intergenic
TCAGGTTGAAGTGGAGCAAC+TGG	0.438563	5:+1994123	None:intergenic
AGATGACGAGGTGACTCATC+TGG	0.442925	5:+1994072	None:intergenic
TCATCTGGCTCGACAGCAAC+AGG	0.445296	5:+1994087	None:intergenic
CGGTCAAATCATCCTGTAGA+TGG	0.447244	5:+1994298	None:intergenic
CAGCGTATTGTTGAAACTGC+TGG	0.453044	5:-1994234	MsG0580024204.01.T01:CDS
AAAGGTTCTTACCAAGTATG+AGG	0.458529	5:-1994160	MsG0580024204.01.T01:CDS
TCCAGCTGGCTTTCTAATCA+AGG	0.459682	5:+1994029	None:intergenic
AATTTCATCATTAACATTCA+GGG	0.462722	5:+1994185	None:intergenic
TGTCATCATTGTTCCCTCCC+TGG	0.472644	5:+1993992	None:intergenic
TGTTAATGATGAAATTCTAA+AGG	0.474583	5:-1994178	MsG0580024204.01.T01:CDS
TGGTTTCAACTCCTCATACT+TGG	0.475462	5:+1994149	None:intergenic
TTGTTCCCTCCCTGGACACC+AGG	0.478367	5:+1994000	None:intergenic
AGCGTATTGTTGAAACTGCT+GGG	0.485271	5:-1994233	MsG0580024204.01.T01:CDS
CCAGTCAATGTTCCATGTTA+GGG	0.490517	5:-1994374	None:intergenic
TCATCTTGATGATGCTAAAG+AGG	0.491881	5:-1994055	MsG0580024204.01.T01:CDS
TGAAGTGGAGCAACTGGAGG+TGG	0.494688	5:+1994129	None:intergenic
ACACCAGGTCTTGATCCAGC+TGG	0.545281	5:+1994015	None:intergenic
ACAGCAACAGGTATCATAGC+TGG	0.557428	5:+1994099	None:intergenic
GGATCAAGACCTGGTGTCCA+GGG	0.564963	5:-1994009	MsG0580024204.01.T01:CDS
AATATGCTCATTCCCTAACA+TGG	0.569093	5:+1994362	None:intergenic
TTAGGTAACTCACAGATGCC+TGG	0.573231	5:+1994336	None:intergenic
TCCTTGATTAGAAAGCCAGC+TGG	0.580335	5:-1994030	MsG0580024204.01.T01:CDS
ATAGCTGGTTCAGGTTGAAG+TGG	0.593332	5:+1994114	None:intergenic
GGGAATGAGCATATTGTACC+AGG	0.595514	5:-1994354	MsG0580024204.01.T01:CDS
CAAGACCTGGTGTCCAGGGA+GGG	0.606209	5:-1994005	MsG0580024204.01.T01:CDS
AGGGAACAATGATGACATGA+TGG	0.620276	5:-1993986	MsG0580024204.01.T01:CDS
AAGCCAGCTGGATCAAGACC+TGG	0.644482	5:-1994018	MsG0580024204.01.T01:CDS
TCAAGACCTGGTGTCCAGGG+AGG	0.644753	5:-1994006	MsG0580024204.01.T01:CDS
ACGACACTGCTGTACAAGTG+TGG	0.646490	5:+1994275	None:intergenic
CAGCAGTTTCAACAATACGC+TGG	0.650943	5:+1994235	None:intergenic
GGTTGAAGTGGAGCAACTGG+AGG	0.659537	5:+1994126	None:intergenic
GCGTATTGTTGAAACTGCTG+GGG	0.671775	5:-1994232	MsG0580024204.01.T01:CDS
ACACTGCTGTACAAGTGTGG+CGG	0.710445	5:+1994278	None:intergenic
TTAGCATCATCAAGATGACG+AGG	0.718165	5:+1994060	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTCATCATTAACATTCA+GGG	+	Chr5:1994060-1994079	None:intergenic	20.0%
!!	TGTTAATGATGAAATTCTAA+AGG	-	Chr5:1994064-1994083	MsG0580024204.01.T01:CDS	20.0%
!!!	ATCTTGATGAAATGATTTTT+GGG	-	Chr5:1994282-1994301	MsG0580024204.01.T01:CDS	20.0%
!	GAATTTCATCATTAACATTC+AGG	+	Chr5:1994061-1994080	None:intergenic	25.0%
!	TGGAAATAAAGATCACATTT+AGG	+	Chr5:1993927-1993946	None:intergenic	25.0%
!!	GATCTTGATGAAATGATTTT+TGG	-	Chr5:1994281-1994300	MsG0580024204.01.T01:CDS	25.0%
!!	TAATGAAGCAATTCTTTTTG+AGG	-	Chr5:1994034-1994053	MsG0580024204.01.T01:CDS	25.0%
!	TGATCTTTATTTCCATCTAC+AGG	-	Chr5:1993932-1993951	MsG0580024204.01.T01:CDS	30.0%
!!	ATGATTTTTGGGAGTAAAGT+TGG	-	Chr5:1994293-1994312	MsG0580024204.01.T01:CDS	30.0%
!!	TGATTTTTGGGAGTAAAGTT+GGG	-	Chr5:1994294-1994313	MsG0580024204.01.T01:CDS	30.0%
	AAAGGTTCTTACCAAGTATG+AGG	-	Chr5:1994082-1994101	MsG0580024204.01.T01:CDS	35.0%
!!	ATTTTTGGGAGTAAAGTTGG+GGG	-	Chr5:1994296-1994315	MsG0580024204.01.T01:CDS	35.0%
!!	GATTTTTGGGAGTAAAGTTG+GGG	-	Chr5:1994295-1994314	MsG0580024204.01.T01:CDS	35.0%
!!	TCATCTTGATGATGCTAAAG+AGG	-	Chr5:1994187-1994206	MsG0580024204.01.T01:CDS	35.0%
	AGCGTATTGTTGAAACTGCT+GGG	-	Chr5:1994009-1994028	MsG0580024204.01.T01:CDS	40.0%
	AGGGAACAATGATGACATGA+TGG	-	Chr5:1994256-1994275	MsG0580024204.01.T01:CDS	40.0%
	TGGTTTCAACTCCTCATACT+TGG	+	Chr5:1994096-1994115	None:intergenic	40.0%
	TTAGCATCATCAAGATGACG+AGG	+	Chr5:1994185-1994204	None:intergenic	40.0%
	ACAGCAACAGGTATCATAGC+TGG	+	Chr5:1994146-1994165	None:intergenic	45.0%
	CAGCAGTTTCAACAATACGC+TGG	+	Chr5:1994010-1994029	None:intergenic	45.0%
	CAGCGTATTGTTGAAACTGC+TGG	-	Chr5:1994008-1994027	MsG0580024204.01.T01:CDS	45.0%
	CGGTCAAATCATCCTGTAGA+TGG	+	Chr5:1993947-1993966	None:intergenic	45.0%
	GCGTATTGTTGAAACTGCTG+GGG	-	Chr5:1994010-1994029	MsG0580024204.01.T01:CDS	45.0%
	GGGAATGAGCATATTGTACC+AGG	-	Chr5:1993888-1993907	MsG0580024204.01.T01:CDS	45.0%
	TCCTTGATTAGAAAGCCAGC+TGG	-	Chr5:1994212-1994231	MsG0580024204.01.T01:CDS	45.0%
	TTAGGTAACTCACAGATGCC+TGG	+	Chr5:1993909-1993928	None:intergenic	45.0%
!	ACAGGTATCATAGCTGGTTC+AGG	+	Chr5:1994140-1994159	None:intergenic	45.0%
!!	ATAGCTGGTTCAGGTTGAAG+TGG	+	Chr5:1994131-1994150	None:intergenic	45.0%
!!	TCCAGCTGGCTTTCTAATCA+AGG	+	Chr5:1994216-1994235	None:intergenic	45.0%
	ACACTGCTGTACAAGTGTGG+CGG	+	Chr5:1993967-1993986	None:intergenic	50.0%
	ACGACACTGCTGTACAAGTG+TGG	+	Chr5:1993970-1993989	None:intergenic	50.0%
	AGATGACGAGGTGACTCATC+TGG	+	Chr5:1994173-1994192	None:intergenic	50.0%
	TGTCATCATTGTTCCCTCCC+TGG	+	Chr5:1994253-1994272	None:intergenic	50.0%
!!	TCAGGTTGAAGTGGAGCAAC+TGG	+	Chr5:1994122-1994141	None:intergenic	50.0%
	AAGCCAGCTGGATCAAGACC+TGG	-	Chr5:1994224-1994243	MsG0580024204.01.T01:CDS	55.0%
	ACACCAGGTCTTGATCCAGC+TGG	+	Chr5:1994230-1994249	None:intergenic	55.0%
	GGATCAAGACCTGGTGTCCA+GGG	-	Chr5:1994233-1994252	MsG0580024204.01.T01:CDS	55.0%
	GGTTGAAGTGGAGCAACTGG+AGG	+	Chr5:1994119-1994138	None:intergenic	55.0%
	TGAAGTGGAGCAACTGGAGG+TGG	+	Chr5:1994116-1994135	None:intergenic	55.0%
	TGGATCAAGACCTGGTGTCC+AGG	-	Chr5:1994232-1994251	MsG0580024204.01.T01:CDS	55.0%
!	AAAGTTGGGGGCGCATCAGA+TGG	-	Chr5:1994308-1994327	MsG0580024204.01.T01:CDS	55.0%
!	TCATCTGGCTCGACAGCAAC+AGG	+	Chr5:1994158-1994177	None:intergenic	55.0%
	CAAGACCTGGTGTCCAGGGA+GGG	-	Chr5:1994237-1994256	MsG0580024204.01.T01:CDS	60.0%
	TCAAGACCTGGTGTCCAGGG+AGG	-	Chr5:1994236-1994255	MsG0580024204.01.T01:CDS	60.0%
	TTGTTCCCTCCCTGGACACC+AGG	+	Chr5:1994245-1994264	None:intergenic	60.0%
!	GTTGGGGGCGCATCAGATGG+AGG	-	Chr5:1994311-1994330	MsG0580024204.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	1993882	1994382	1993882	ID=MsG0580024204.01;Name=MsG0580024204.01
Chr5	mRNA	1993882	1994382	1993882	ID=MsG0580024204.01.T01;Parent=MsG0580024204.01;Name=MsG0580024204.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|166
Chr5	exon	1993882	1994382	1993882	ID=MsG0580024204.01.T01:exon:23622;Parent=MsG0580024204.01.T01
Chr5	CDS	1993882	1994382	1993882	ID=MsG0580024204.01.T01:cds;Parent=MsG0580024204.01.T01

Gene Sequence

>MsG0580024204.01.T01

ATGTTAGGGAATGAGCATATTGTACCAGGCATCTGTGAGTTACCTAAATGTGATCTTTATTTCCATCTACAGGATGATTTGACCGCCACACTTGTACAGCAGTGTCGTCGCTCTCAAATTACTGTCCAGCGTATTGTTGAAACTGCTGGGGATAATGAAGCAATTCTTTTTGAGGCCCTGAATGTTAATGATGAAATTCTAAAGGTTCTTACCAAGTATGAGGAGTTGAAACCACCTCCAGTTGCTCCACTTCAACCTGAACCAGCTATGATACCTGTTGCTGTCGAGCCAGATGAGTCACCTCGTCATCTTGATGATGCTAAAGAGGACTCCTTGATTAGAAAGCCAGCTGGATCAAGACCTGGTGTCCAGGGAGGGAACAATGATGACATGATGGATGATCTTGATGAAATGATTTTTGGGAGTAAAGTTGGGGGCGCATCAGATGGAGGACATGACACAAAAAAACAATCTTCTAAAGATGATCTCATCTCCTTCTAA

Protein sequence

>MsG0580024204.01.T01

MLGNEHIVPGICELPKCDLYFHLQDDLTATLVQQCRRSQITVQRIVETAGDNEAILFEALNVNDEILKVLTKYEELKPPPVAPLQPEPAMIPVAVEPDESPRHLDDAKEDSLIRKPAGSRPGVQGGNNDDMMDDLDEMIFGSKVGGASDGGHDTKKQSSKDDLISF*