Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000090.01 | MsG0180000091.01 | 0.839528 | 1.501644e-57 | 2.079338e-54 |
| MsG0180000090.01 | MsG0180000470.01 | 0.805700 | 1.176346e-49 | 6.265831e-47 |
| MsG0180000090.01 | MsG0180000652.01 | 0.800817 | 1.206036e-48 | 5.669792e-46 |
| MsG0180000090.01 | MsG0180001971.01 | 0.823024 | 1.730721e-53 | 1.472967e-50 |
| MsG0180000090.01 | MsG0180003193.01 | 0.801656 | 8.121770e-49 | 3.899995e-46 |
| MsG0180000090.01 | MsG0180004765.01 | 0.811461 | 6.920109e-51 | 4.286257e-48 |
| MsG0180000090.01 | MsG0280007066.01 | 0.800099 | 1.689349e-48 | 7.798615e-46 |
| MsG0180000090.01 | MsG0280010807.01 | 0.800136 | 1.660271e-48 | 7.671691e-46 |
| MsG0180000090.01 | MsG0380016285.01 | 0.801060 | 1.075973e-48 | 5.089957e-46 |
| MsG0180000090.01 | MsG0380017220.01 | 0.811539 | 6.657180e-51 | 4.131842e-48 |
| MsG0180000090.01 | MsG0480021925.01 | 0.801494 | 8.767212e-49 | 4.192910e-46 |
| MsG0180000090.01 | MsG0480021948.01 | 0.800853 | 1.185776e-48 | 5.579983e-46 |
| MsG0180000090.01 | MsG0480023251.01 | 0.820854 | 5.505441e-53 | 4.406916e-50 |
| MsG0180000090.01 | MsG0580024038.01 | 0.802136 | 6.474994e-49 | 3.147394e-46 |
| MsG0180000090.01 | MsG0680030880.01 | 0.811263 | 7.639126e-51 | 4.706883e-48 |
| MsG0180000090.01 | MsG0680032157.01 | 0.810514 | 1.109780e-50 | 6.703056e-48 |
| MsG0180000090.01 | MsG0780038401.01 | 0.801735 | 7.825776e-49 | 3.765306e-46 |
| MsG0180000090.01 | MsG0880043157.01 | 0.801782 | 7.655309e-49 | 3.687722e-46 |
| MsG0180000090.01 | MsG0880045372.01 | 0.802016 | 6.852390e-49 | 3.320697e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCAGCTGTGCCCAACAAA+AGG | 0.459201 | 1:-1325068 | None:intergenic |
| GTACAAGAGCATGCTGGTGC+TGG | 0.473862 | 1:+1325170 | MsG0180000090.01.T01:CDS |
| GCACCAGCATGCTCTTGTAC+TGG | 0.474798 | 1:-1325167 | None:intergenic |
| TGATTGGAAACCTCAGGACT+TGG | 0.494236 | 1:-1325228 | None:intergenic |
| GACAGTGCTACAAGGGGTAA+TGG | 0.494266 | 1:-1325135 | None:intergenic |
| TTGAATCATCGTATGCAACT+TGG | 0.503216 | 1:+1325198 | MsG0180000090.01.T01:CDS |
| CTTTCAATAAACTACTTAGC+CGG | 0.514461 | 1:-1325010 | None:intergenic |
| TTGTTGGGCACAGCTGGAAG+TGG | 0.517836 | 1:+1325072 | MsG0180000090.01.T01:CDS |
| CTTAGCCGGCTTGCAGCTGA+AGG | 0.537332 | 1:-1324996 | None:intergenic |
| GGTTCTGAGACAGTGCTACA+AGG | 0.544598 | 1:-1325143 | None:intergenic |
| CTACAAGGGGTAATGGAGGT+TGG | 0.550986 | 1:-1325128 | None:intergenic |
| CAACAAAAGGCTGCTCGAGT+AGG | 0.570625 | 1:-1325055 | None:intergenic |
| CCAGCATGCTCTTGTACTGG+TGG | 0.571309 | 1:-1325164 | None:intergenic |
| CAAAAGGCTGCTCGAGTAGG+TGG | 0.573912 | 1:-1325052 | None:intergenic |
| GAAGTGGATATACGTTAAAG+TGG | 0.575733 | 1:+1325088 | MsG0180000090.01.T01:CDS |
| TGGTTCAATGCCAAGTCCTG+AGG | 0.592965 | 1:+1325218 | MsG0180000090.01.T01:CDS |
| GTTCTGAGACAGTGCTACAA+GGG | 0.593947 | 1:-1325142 | None:intergenic |
| AGAGCATGCTGGTGCTGGCA+TGG | 0.596415 | 1:+1325175 | MsG0180000090.01.T01:CDS |
| AGTAGGTGGTAGAAAATGAG+TGG | 0.610399 | 1:-1325038 | None:intergenic |
| TCATGCCTTCAGCTGCAAGC+CGG | 0.612888 | 1:+1324991 | None:intergenic |
| CCACCAGTACAAGAGCATGC+TGG | 0.634792 | 1:+1325164 | MsG0180000090.01.T01:CDS |
| AGTGCTACAAGGGGTAATGG+AGG | 0.670124 | 1:-1325132 | None:intergenic |
| TTCTGAGACAGTGCTACAAG+GGG | 0.670488 | 1:-1325141 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTTTCAATAAACTACTTAGC+CGG | - | Chr1:1325013-1325032 | None:intergenic | 30.0% | |
| GAAGTGGATATACGTTAAAG+TGG | + | Chr1:1325088-1325107 | MsG0180000090.01.T01:CDS | 35.0% | |
| TTGAATCATCGTATGCAACT+TGG | + | Chr1:1325198-1325217 | MsG0180000090.01.T01:CDS | 35.0% | |
| AGTAGGTGGTAGAAAATGAG+TGG | - | Chr1:1325041-1325060 | None:intergenic | 40.0% | |
| ! | TACTCGAGCAGCCTTTTGTT+GGG | + | Chr1:1325057-1325076 | MsG0180000090.01.T01:CDS | 45.0% |
| !! | GTTCTGAGACAGTGCTACAA+GGG | - | Chr1:1325145-1325164 | None:intergenic | 45.0% |
| !! | TTCTGAGACAGTGCTACAAG+GGG | - | Chr1:1325144-1325163 | None:intergenic | 45.0% |
| CAACAAAAGGCTGCTCGAGT+AGG | - | Chr1:1325058-1325077 | None:intergenic | 50.0% | |
| GACAGTGCTACAAGGGGTAA+TGG | - | Chr1:1325138-1325157 | None:intergenic | 50.0% | |
| TTCCAGCTGTGCCCAACAAA+AGG | - | Chr1:1325071-1325090 | None:intergenic | 50.0% | |
| ! | AGTGCTACAAGGGGTAATGG+AGG | - | Chr1:1325135-1325154 | None:intergenic | 50.0% |
| ! | CTACTCGAGCAGCCTTTTGT+TGG | + | Chr1:1325056-1325075 | MsG0180000090.01.T01:CDS | 50.0% |
| !! | CTACAAGGGGTAATGGAGGT+TGG | - | Chr1:1325131-1325150 | None:intergenic | 50.0% |
| !! | GGTTCTGAGACAGTGCTACA+AGG | - | Chr1:1325146-1325165 | None:intergenic | 50.0% |
| CAAAAGGCTGCTCGAGTAGG+TGG | - | Chr1:1325055-1325074 | None:intergenic | 55.0% | |
| CCAGCATGCTCTTGTACTGG+TGG | - | Chr1:1325167-1325186 | None:intergenic | 55.0% | |
| GCACCAGCATGCTCTTGTAC+TGG | - | Chr1:1325170-1325189 | None:intergenic | 55.0% | |
| TTGTTGGGCACAGCTGGAAG+TGG | + | Chr1:1325072-1325091 | MsG0180000090.01.T01:CDS | 55.0% | |
| ! | AGCCTTTTGTTGGGCACAGC+TGG | + | Chr1:1325066-1325085 | MsG0180000090.01.T01:CDS | 55.0% |
| ! | CCACCAGTACAAGAGCATGC+TGG | + | Chr1:1325164-1325183 | MsG0180000090.01.T01:CDS | 55.0% |
| ! | GTACAAGAGCATGCTGGTGC+TGG | + | Chr1:1325170-1325189 | MsG0180000090.01.T01:CDS | 55.0% |
| !! | AGAGCATGCTGGTGCTGGCA+TGG | + | Chr1:1325175-1325194 | MsG0180000090.01.T01:CDS | 60.0% |
| !! | CTTAGCCGGCTTGCAGCTGA+AGG | - | Chr1:1324999-1325018 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 1324993 | 1325238 | 1324993 | ID=MsG0180000090.01;Name=MsG0180000090.01 |
| Chr1 | mRNA | 1324993 | 1325238 | 1324993 | ID=MsG0180000090.01.T01;Parent=MsG0180000090.01;Name=MsG0180000090.01.T01;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|81 |
| Chr1 | exon | 1324993 | 1325238 | 1324993 | ID=MsG0180000090.01.T01:exon:7337;Parent=MsG0180000090.01.T01 |
| Chr1 | CDS | 1324993 | 1325238 | 1324993 | ID=MsG0180000090.01.T01:cds;Parent=MsG0180000090.01.T01 |
| Gene Sequence |
| Protein sequence |
