AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000183.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000183.01.T01 MTR_1g021895 99.138 232 2 0 1 232 1 232 9.23e-173 474
MsG0180000183.01.T01 MTR_5g019300 56.067 239 95 4 1 231 1 237 1.52e-77 233
MsG0180000183.01.T01 MTR_8g465910 33.186 226 120 9 19 230 34 242 3.21e-30 112
MsG0180000183.01.T01 MTR_2g090325 52.222 90 37 2 21 110 10 93 8.27e-30 110
MsG0180000183.01.T01 MTR_2g090330 48.958 96 44 2 22 112 22 117 1.48e-28 106
MsG0180000183.01.T01 MTR_2g090330 48.958 96 44 2 22 112 25 120 1.71e-28 105
MsG0180000183.01.T01 MTR_7g093390 51.163 86 40 1 30 115 170 253 5.38e-28 110
MsG0180000183.01.T01 MTR_2g090295 50.000 94 39 2 25 110 35 128 4.05e-27 104
MsG0180000183.01.T01 MTR_1g054210 41.379 116 65 2 3 115 93 208 7.91e-27 103
MsG0180000183.01.T01 MTR_1g054280 47.191 89 44 3 25 111 71 158 1.45e-25 100
MsG0180000183.01.T01 MTR_1g054260 38.843 121 64 2 7 117 64 184 1.31e-24 97.1
MsG0180000183.01.T01 MTR_4g088870 38.406 138 67 5 30 149 32 169 4.11e-24 95.5
MsG0180000183.01.T01 MTR_4g029440 38.793 116 64 2 30 138 126 241 2.43e-23 97.4
MsG0180000183.01.T01 MTR_2g013550 52.857 70 26 1 26 88 23 92 2.88e-23 94.4
MsG0180000183.01.T01 MTR_4g029440 38.793 116 64 2 30 138 132 247 2.89e-23 97.4
MsG0180000183.01.T01 MTR_4g088875 48.889 90 35 3 30 108 31 120 4.04e-23 94.0
MsG0180000183.01.T01 MTR_1g054465 42.308 104 46 3 19 108 40 143 4.41e-23 92.8
MsG0180000183.01.T01 MTR_2g061230 38.636 88 53 1 30 116 107 194 2.88e-19 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000183.01.T01 AT1G19310 67.347 196 47 6 30 215 21 209 9.10e-85 251
MsG0180000183.01.T01 AT2G23780 55.760 217 86 5 20 231 16 227 3.96e-79 237
MsG0180000183.01.T01 AT2G23780 55.760 217 86 5 20 231 16 227 3.96e-79 237
MsG0180000183.01.T01 AT2G23780 55.760 217 86 5 20 231 16 227 3.96e-79 237
MsG0180000183.01.T01 AT1G74990 55.639 133 56 2 20 151 7 137 1.94e-48 156
MsG0180000183.01.T01 AT4G03510 31.624 234 135 7 10 230 26 247 4.62e-32 117
MsG0180000183.01.T01 AT4G03510 31.624 234 135 7 10 230 26 247 4.62e-32 117
MsG0180000183.01.T01 AT4G03510 31.624 234 135 7 10 230 26 247 4.62e-32 117
MsG0180000183.01.T01 AT4G03510 31.624 234 135 7 10 230 26 247 4.62e-32 117
MsG0180000183.01.T01 AT4G27470 44.828 116 51 3 25 127 37 152 4.80e-27 104
MsG0180000183.01.T01 AT4G28270 46.875 96 32 3 28 110 17 106 1.93e-25 99.0
MsG0180000183.01.T01 AT2G42030 45.055 91 47 2 25 115 134 221 3.93e-25 102
MsG0180000183.01.T01 AT3G58030 45.055 91 47 3 25 115 132 219 1.74e-23 98.2
MsG0180000183.01.T01 AT3G58030 45.055 91 47 3 25 115 132 219 1.74e-23 98.2
MsG0180000183.01.T01 AT3G58030 45.055 91 47 3 25 115 132 219 1.74e-23 98.2
MsG0180000183.01.T01 AT3G58030 45.055 91 47 3 25 115 132 219 1.74e-23 98.2
MsG0180000183.01.T01 AT2G44410 40.659 91 51 2 28 116 121 210 3.18e-19 85.9

Find 84 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAAATTGGTTCCTTTATA+TGG 0.186055 1:+2569658 MsG0180000183.01.T01:CDS
TCAGGTGGAGGTGCAGTTTC+AGG 0.199841 1:-2569760 None:intergenic
GGAGCTGCTGGTTTCCCTTA+TGG 0.211929 1:+2569964 MsG0180000183.01.T01:CDS
CCAAATCCATGTTGAGAAAA+AGG 0.224751 1:-2569790 None:intergenic
GAGCTGCTGGTTTCCCTTAT+GGG 0.235535 1:+2569965 MsG0180000183.01.T01:CDS
TTTGGATTCACAGGTGGATT+AGG 0.251366 1:+2569808 MsG0180000183.01.T01:CDS
TATACCTATGAGATGGTTAT+TGG 0.256037 1:+2570372 MsG0180000183.01.T01:three_prime_UTR
TTCACATTTGCTGCCGGATT+TGG 0.272552 1:+2569868 MsG0180000183.01.T01:CDS
TACAGATGGCAAGTGGGTTT+GGG 0.276615 1:+2569419 MsG0180000183.01.T01:intron
GCTGCCGGATTTGGTGGATT+GGG 0.286886 1:+2569877 MsG0180000183.01.T01:CDS
TTGGATTCACAGGTGGATTA+GGG 0.303936 1:+2569809 MsG0180000183.01.T01:CDS
GATGATTGAGGAGGAGAAAT+TGG 0.306323 1:+2569645 MsG0180000183.01.T01:CDS
CCGGCAACAACTGCAAGATT+TGG 0.331916 1:+2569841 MsG0180000183.01.T01:CDS
TGACCACACAAAGTAATAAT+AGG 0.332316 1:-2569547 None:intergenic
TTACAGATGGCAAGTGGGTT+TGG 0.332494 1:+2569418 MsG0180000183.01.T01:intron
TGCTGCCGGATTTGGTGGAT+TGG 0.350270 1:+2569876 MsG0180000183.01.T01:CDS
GAGCTTATGTTTGGTTATAT+CGG 0.351235 1:-2570143 None:intergenic
CCAAATCTTGCAGTTGTTGC+CGG 0.353767 1:-2569841 None:intergenic
ATGCAACTGAAAGTTAAATA+AGG 0.360551 1:-2569914 None:intergenic
GTTGGCCTTGTCTTTACAAA+TGG 0.380746 1:+2569578 MsG0180000183.01.T01:CDS
TCCGAGGTCGAAATCTATTC+CGG 0.384427 1:+2569702 MsG0180000183.01.T01:CDS
AGAAATTGGTTCCTTTATAT+GGG 0.388973 1:+2569659 MsG0180000183.01.T01:CDS
GTCTTTCCTCTCCCATATAA+AGG 0.393313 1:-2569670 None:intergenic
GCCATGTATGGTGGAGCTGC+TGG 0.395626 1:+2569952 MsG0180000183.01.T01:CDS
TACTCGAGTAAGGAGGGCTT+GGG 0.406878 1:-2569462 None:intergenic
CTGCCGGATTTGGTGGATTG+GGG 0.407237 1:+2569878 MsG0180000183.01.T01:CDS
CGGCAACAACTGCAAGATTT+GGG 0.413932 1:+2569842 MsG0180000183.01.T01:CDS
TCAATCATCGCTTTACAAAC+CGG 0.418409 1:-2569631 None:intergenic
GTTTGATAAGAGCTTATGTT+TGG 0.426045 1:-2570152 None:intergenic
TTTCACGGTGGGGCCATGTA+TGG 0.430081 1:+2569940 MsG0180000183.01.T01:CDS
TTATTATTATTACTCGAGTA+AGG 0.430340 1:-2569472 None:intergenic
AATTCCAATAACCATCTCAT+AGG 0.437495 1:-2570376 None:intergenic
CAACATGGATTTGGATTCAC+AGG 0.439413 1:+2569799 MsG0180000183.01.T01:CDS
AATAATAATAACAGTGATGC+TGG 0.448158 1:+2569484 MsG0180000183.01.T01:CDS
ACATTTGCTGCCGGATTTGG+TGG 0.448518 1:+2569871 MsG0180000183.01.T01:CDS
TTACTCGAGTAAGGAGGGCT+TGG 0.452550 1:-2569463 None:intergenic
CTCCCAGTCACGAGAATGCC+CGG 0.453449 1:+2569612 MsG0180000183.01.T01:CDS
CCGAGGTCGAAATCTATTCC+GGG 0.463757 1:+2569703 MsG0180000183.01.T01:CDS
AAACCGGGCATTCTCGTGAC+TGG 0.466413 1:-2569615 None:intergenic
TTATTTAACTTTCAGTTGCA+TGG 0.466871 1:+2569916 MsG0180000183.01.T01:CDS
AATGCATTAGAAAACCCATA+AGG 0.474862 1:-2569979 None:intergenic
GTGTGGTACTTGCTTTACTT+TGG 0.475767 1:+2570094 MsG0180000183.01.T01:CDS
GATCCTATTATTACTTTGTG+TGG 0.482342 1:+2569544 MsG0180000183.01.T01:CDS
AAAATAATAATATGAAGAAA+AGG 0.483607 1:-2570185 None:intergenic
ATGCATTAGAAAACCCATAA+GGG 0.492861 1:-2569978 None:intergenic
TTGGTTCCTTTATATGGGAG+AGG 0.500872 1:+2569664 MsG0180000183.01.T01:CDS
GGGAATTTCACATTTGCTGC+CGG 0.503544 1:+2569862 MsG0180000183.01.T01:CDS
ACCAGCAGCTCCACCATACA+TGG 0.508328 1:-2569953 None:intergenic
AATATCCCTCATCGTCCTGC+CGG 0.511349 1:+2569730 MsG0180000183.01.T01:CDS
CACGGTGGGGCCATGTATGG+TGG 0.514031 1:+2569943 MsG0180000183.01.T01:CDS
AAAGGATTTGATTCAGGTGG+AGG 0.516952 1:-2569772 None:intergenic
TGCCGGATTTGGTGGATTGG+GGG 0.524202 1:+2569879 MsG0180000183.01.T01:CDS
TATCCTCTGCACACAGGTCA+AGG 0.531811 1:+2570024 MsG0180000183.01.T01:CDS
GTTTCAGGTCTCTGACCGGC+AGG 0.546908 1:-2569745 None:intergenic
TATTATTACTCGAGTAAGGA+GGG 0.550496 1:-2569468 None:intergenic
TGCAGTTTCAGGTCTCTGAC+CGG 0.558086 1:-2569749 None:intergenic
GGATTCACAGGTGGATTAGG+GGG 0.558493 1:+2569811 MsG0180000183.01.T01:CDS
CAATCATCGCTTTACAAACC+GGG 0.560492 1:-2569630 None:intergenic
CATGGATTTGGATTCACAGG+TGG 0.560706 1:+2569802 MsG0180000183.01.T01:CDS
ACAGATGGCAAGTGGGTTTG+GGG 0.562002 1:+2569420 MsG0180000183.01.T01:intron
CTCTGACCGGCAGGACGATG+AGG 0.563898 1:-2569736 None:intergenic
AACAAGATTATTATCTGAAG+AGG 0.566850 1:+2570049 MsG0180000183.01.T01:CDS
AACCGGGCATTCTCGTGACT+GGG 0.572439 1:-2569614 None:intergenic
ACTCGAGTAAGGAGGGCTTG+GGG 0.581186 1:-2569461 None:intergenic
AACTGAAAGTTAAATAAGGA+TGG 0.582621 1:-2569910 None:intergenic
TTATTATTACTCGAGTAAGG+AGG 0.589072 1:-2569469 None:intergenic
GCCGGATTTGGTGGATTGGG+GGG 0.591932 1:+2569880 MsG0180000183.01.T01:CDS
CTCGAGTAAGGAGGGCTTGG+GGG 0.592036 1:-2569460 None:intergenic
TGCCCTTGACCTGTGTGCAG+AGG 0.596436 1:-2570027 None:intergenic
CATGATATATACCTATGAGA+TGG 0.608365 1:+2570365 MsG0180000183.01.T01:three_prime_UTR
ATCCTCTGCACACAGGTCAA+GGG 0.610816 1:+2570025 MsG0180000183.01.T01:CDS
GATCGATAAACTAATTAGAG+AGG 0.613829 1:-2570257 None:intergenic
TCTGACCGGCAGGACGATGA+GGG 0.614884 1:-2569735 None:intergenic
CCCGGAATAGATTTCGACCT+CGG 0.616864 1:-2569703 None:intergenic
TGGATTCACAGGTGGATTAG+GGG 0.620475 1:+2569810 MsG0180000183.01.T01:CDS
GGTTTGGGGAATCAACAAGC+AGG 0.622199 1:+2569434 MsG0180000183.01.T01:CDS
GTACCTGAAGAGAAACAAAA+CGG 0.624667 1:-2568453 None:intergenic
CATAGATATCCTCTGCACAC+AGG 0.625097 1:+2570018 MsG0180000183.01.T01:CDS
CGATGAGGGATATTAACACC+CGG 0.632199 1:-2569721 None:intergenic
GGTTTGTAAAGCGATGATTG+AGG 0.642603 1:+2569633 MsG0180000183.01.T01:CDS
TTGTAAAGCGATGATTGAGG+AGG 0.645599 1:+2569636 MsG0180000183.01.T01:CDS
TGAAGCCATTTGTAAAGACA+AGG 0.646514 1:-2569583 None:intergenic
GCAGAGGATATCTATGAACA+TGG 0.651368 1:-2570011 None:intergenic
GAAAGACTTCAACTGATCCG+AGG 0.691424 1:+2569686 MsG0180000183.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATAATATGAAGAAA+AGG - Chr1:2570188-2570207 None:intergenic 10.0%
!!! ATCTTTTTTTTTTTTTTTGA+GGG + Chr1:2568820-2568839 MsG0180000183.01.T01:intron 10.0%
!! AAATAAAAAATTGAGGAAAA+GGG - Chr1:2568479-2568498 None:intergenic 15.0%
!! TAAATAAAAAATTGAGGAAA+AGG - Chr1:2568480-2568499 None:intergenic 15.0%
!! TATAGAAATAGTTTATACAT+AGG + Chr1:2569139-2569158 MsG0180000183.01.T01:intron 15.0%
!!! ATATTATTATTTTGTAACTG+TGG + Chr1:2570195-2570214 MsG0180000183.01.T01:three_prime_UTR 15.0%
!!! CATCTTTTTTTTTTTTTTTG+AGG + Chr1:2568819-2568838 MsG0180000183.01.T01:intron 15.0%
!! AAACATCAAATAAAGTCAAA+TGG - Chr1:2569285-2569304 None:intergenic 20.0%
!! AAGATTTAACAAAATGGATA+AGG - Chr1:2568667-2568686 None:intergenic 20.0%
!! AATAAAAAATTGAGGAAAAG+GGG - Chr1:2568478-2568497 None:intergenic 20.0%
!! TAAAGCTAAGAATGTTATAA+AGG - Chr1:2568714-2568733 None:intergenic 20.0%
!! TTATTATTATTACTCGAGTA+AGG - Chr1:2569475-2569494 None:intergenic 20.0%
!! TTCAATTGAAATCTGAATTA+GGG + Chr1:2568601-2568620 MsG0180000183.01.T01:intron 20.0%
!! TTGTTGAAGATTTAACAAAA+TGG - Chr1:2568673-2568692 None:intergenic 20.0%
!! TTTCAATTGAAATCTGAATT+AGG + Chr1:2568600-2568619 MsG0180000183.01.T01:intron 20.0%
!!! AATTAAGTTTTTATGTGCAA+GGG + Chr1:2568946-2568965 MsG0180000183.01.T01:intron 20.0%
!!! GTTTTCAGCTTATTTTAATA+AGG + Chr1:2569069-2569088 MsG0180000183.01.T01:intron 20.0%
!!! TAATTAAGTTTTTATGTGCA+AGG + Chr1:2568945-2568964 MsG0180000183.01.T01:intron 20.0%
!!! TTTAATTTAATTTTCGTCAC+TGG + Chr1:2568793-2568812 MsG0180000183.01.T01:intron 20.0%
! AACAAGATTATTATCTGAAG+AGG + Chr1:2570049-2570068 MsG0180000183.01.T01:CDS 25.0%
! AACTGAAAGTTAAATAAGGA+TGG - Chr1:2569913-2569932 None:intergenic 25.0%
! AATAAGCTGAAAACAACTTA+TGG - Chr1:2569063-2569082 None:intergenic 25.0%
! AATAATAATAACAGTGATGC+TGG + Chr1:2569484-2569503 MsG0180000183.01.T01:CDS 25.0%
! ACAGTTTAATTAAGCACTTA+TGG - Chr1:2569188-2569207 None:intergenic 25.0%
! ATGCAACTGAAAGTTAAATA+AGG - Chr1:2569917-2569936 None:intergenic 25.0%
! CAAAAGATAAAATGAAGTCA+TGG - Chr1:2569118-2569137 None:intergenic 25.0%
! TAGCTTATGAAAACAACATT+TGG + Chr1:2569032-2569051 MsG0180000183.01.T01:intron 25.0%
!! AGAAATTGGTTCCTTTATAT+GGG + Chr1:2569659-2569678 MsG0180000183.01.T01:CDS 25.0%
!! TTATTTAACTTTCAGTTGCA+TGG + Chr1:2569916-2569935 MsG0180000183.01.T01:CDS 25.0%
!!! GATGTTTGTTATTTTTTGTG+TGG + Chr1:2570077-2570096 MsG0180000183.01.T01:CDS 25.0%
!!! TTATTATTTTGTAACTGTGG+AGG + Chr1:2570198-2570217 MsG0180000183.01.T01:three_prime_UTR 25.0%
!!! TTCAGCTTATTTTAATAAGG+TGG + Chr1:2569072-2569091 MsG0180000183.01.T01:intron 25.0%
!!! TTGGGATTTTATTTTACAGA+TGG + Chr1:2569405-2569424 MsG0180000183.01.T01:intron 25.0%
!!! TTTTGCTATAGAAATAGCTT+AGG + Chr1:2569302-2569321 MsG0180000183.01.T01:intron 25.0%
AACACATATACATAGCAAAC+AGG - Chr1:2568749-2568768 None:intergenic 30.0%
AATGCATTAGAAAACCCATA+AGG - Chr1:2569982-2570001 None:intergenic 30.0%
AATTCCAATAACCATCTCAT+AGG - Chr1:2570379-2570398 None:intergenic 30.0%
ATCGAAATTCGAAGAAATCA+AGG - Chr1:2568552-2568571 None:intergenic 30.0%
ATGCATTAGAAAACCCATAA+GGG - Chr1:2569981-2570000 None:intergenic 30.0%
CATGATATATACCTATGAGA+TGG + Chr1:2570365-2570384 MsG0180000183.01.T01:three_prime_UTR 30.0%
CTTATTTATGTAAGCTCTCT+TGG + Chr1:2569243-2569262 MsG0180000183.01.T01:intron 30.0%
GAGCTTATGTTTGGTTATAT+CGG - Chr1:2570146-2570165 None:intergenic 30.0%
GATCCTATTATTACTTTGTG+TGG + Chr1:2569544-2569563 MsG0180000183.01.T01:CDS 30.0%
GATCGATAAACTAATTAGAG+AGG - Chr1:2570260-2570279 None:intergenic 30.0%
GTTTGATAAGAGCTTATGTT+TGG - Chr1:2570155-2570174 None:intergenic 30.0%
TAAACTGTTTATCCCTAACA+AGG + Chr1:2569369-2569388 MsG0180000183.01.T01:intron 30.0%
TATACCTATGAGATGGTTAT+TGG + Chr1:2570372-2570391 MsG0180000183.01.T01:three_prime_UTR 30.0%
TATTATTACTCGAGTAAGGA+GGG - Chr1:2569471-2569490 None:intergenic 30.0%
TGACCACACAAAGTAATAAT+AGG - Chr1:2569550-2569569 None:intergenic 30.0%
TGAGAAAAAGGATTTGATTC+AGG - Chr1:2569781-2569800 None:intergenic 30.0%
TTATTATTACTCGAGTAAGG+AGG - Chr1:2569472-2569491 None:intergenic 30.0%
! GAGAAATTGGTTCCTTTATA+TGG + Chr1:2569658-2569677 MsG0180000183.01.T01:CDS 30.0%
! TCAAATCCTTTTTCTCAACA+TGG + Chr1:2569784-2569803 MsG0180000183.01.T01:CDS 30.0%
! TTTGCAATTCGATTTTCCAA+CGG + Chr1:2568512-2568531 MsG0180000183.01.T01:intron 30.0%
!! TTATTTTACAGATGGCAAGT+GGG + Chr1:2569413-2569432 MsG0180000183.01.T01:intron 30.0%
!!! AGCTTATTTTAATAAGGTGG+AGG + Chr1:2569075-2569094 MsG0180000183.01.T01:intron 30.0%
!!! GCTTATTTTAATAAGGTGGA+GGG + Chr1:2569076-2569095 MsG0180000183.01.T01:intron 30.0%
!!! TTTATTTTACAGATGGCAAG+TGG + Chr1:2569412-2569431 MsG0180000183.01.T01:intron 30.0%
AAGCACTTGTGAGACTATTT+GGG + Chr1:2569008-2569027 MsG0180000183.01.T01:intron 35.0%
CCAAATCCATGTTGAGAAAA+AGG - Chr1:2569793-2569812 None:intergenic 35.0%
GAAAAAGGATTTGATTCAGG+TGG - Chr1:2569778-2569797 None:intergenic 35.0%
GTACCTGAAGAGAAACAAAA+CGG - Chr1:2568456-2568475 None:intergenic 35.0%
TAAGCACTTGTGAGACTATT+TGG + Chr1:2569007-2569026 MsG0180000183.01.T01:intron 35.0%
TCAATCATCGCTTTACAAAC+CGG - Chr1:2569634-2569653 None:intergenic 35.0%
TGAAGCCATTTGTAAAGACA+AGG - Chr1:2569586-2569605 None:intergenic 35.0%
! CCTTTTTCTCAACATGGATT+TGG + Chr1:2569790-2569809 MsG0180000183.01.T01:CDS 35.0%
! CTGAATTAGGGTTATGCTTT+TGG + Chr1:2568613-2568632 MsG0180000183.01.T01:intron 35.0%
! GGGTTTTCTAATGCATTTCA+CGG + Chr1:2569985-2570004 MsG0180000183.01.T01:CDS 35.0%
! TTTCAGTTGCATGGTTTTCA+CGG + Chr1:2569925-2569944 MsG0180000183.01.T01:CDS 35.0%
! TTTTCTAATGCATTTCACGG+TGG + Chr1:2569988-2570007 MsG0180000183.01.T01:CDS 35.0%
!! TTCACATTACAGCCTTGTTA+GGG - Chr1:2569384-2569403 None:intergenic 35.0%
!!! TTGAGGTTTTGACTTCAACA+AGG + Chr1:2568410-2568429 MsG0180000183.01.T01:five_prime_UTR 35.0%
!!! TTTTTTTTTGAGGGAGTCAC+TGG + Chr1:2568829-2568848 MsG0180000183.01.T01:intron 35.0%
AAAGGATTTGATTCAGGTGG+AGG - Chr1:2569775-2569794 None:intergenic 40.0%
ACAAGGCTGTAATGTGAAGT+TGG + Chr1:2569386-2569405 MsG0180000183.01.T01:intron 40.0%
CAACATGGATTTGGATTCAC+AGG + Chr1:2569799-2569818 MsG0180000183.01.T01:CDS 40.0%
CAAGGCTGTAATGTGAAGTT+GGG + Chr1:2569387-2569406 MsG0180000183.01.T01:intron 40.0%
CAATCATCGCTTTACAAACC+GGG - Chr1:2569633-2569652 None:intergenic 40.0%
GATGATTGAGGAGGAGAAAT+TGG + Chr1:2569645-2569664 MsG0180000183.01.T01:CDS 40.0%
GCAGAGGATATCTATGAACA+TGG - Chr1:2570014-2570033 None:intergenic 40.0%
GGTTTGTAAAGCGATGATTG+AGG + Chr1:2569633-2569652 MsG0180000183.01.T01:CDS 40.0%
GGTTTGTAAGTTAAGACCGT+TGG - Chr1:2568531-2568550 None:intergenic 40.0%
GTCTTTCCTCTCCCATATAA+AGG - Chr1:2569673-2569692 None:intergenic 40.0%
GTGTGGTACTTGCTTTACTT+TGG + Chr1:2570094-2570113 MsG0180000183.01.T01:CDS 40.0%
TTGGTTCCTTTATATGGGAG+AGG + Chr1:2569664-2569683 MsG0180000183.01.T01:CDS 40.0%
TTGTAAAGCGATGATTGAGG+AGG + Chr1:2569636-2569655 MsG0180000183.01.T01:CDS 40.0%
TTTATGTGCAAGGGTTCGTT+TGG + Chr1:2568955-2568974 MsG0180000183.01.T01:intron 40.0%
! TGTGTGGTCATCTTTTCTGT+TGG + Chr1:2569560-2569579 MsG0180000183.01.T01:CDS 40.0%
! TTTGGATTCACAGGTGGATT+AGG + Chr1:2569808-2569827 MsG0180000183.01.T01:CDS 40.0%
!! CTTCACATTACAGCCTTGTT+AGG - Chr1:2569385-2569404 None:intergenic 40.0%
!! GTTGGCCTTGTCTTTACAAA+TGG + Chr1:2569578-2569597 MsG0180000183.01.T01:CDS 40.0%
!! TTGGATTCACAGGTGGATTA+GGG + Chr1:2569809-2569828 MsG0180000183.01.T01:CDS 40.0%
!!! TCACCGTTTTGTTTCTCTTC+AGG + Chr1:2568450-2568469 MsG0180000183.01.T01:five_prime_UTR 40.0%
CATAGATATCCTCTGCACAC+AGG + Chr1:2570018-2570037 MsG0180000183.01.T01:CDS 45.0%
CATGGATTTGGATTCACAGG+TGG + Chr1:2569802-2569821 MsG0180000183.01.T01:CDS 45.0%
CCAAATCTTGCAGTTGTTGC+CGG - Chr1:2569844-2569863 None:intergenic 45.0%
CGATGAGGGATATTAACACC+CGG - Chr1:2569724-2569743 None:intergenic 45.0%
CGGCAACAACTGCAAGATTT+GGG + Chr1:2569842-2569861 MsG0180000183.01.T01:CDS 45.0%
GAAAGACTTCAACTGATCCG+AGG + Chr1:2569686-2569705 MsG0180000183.01.T01:CDS 45.0%
TCCGAGGTCGAAATCTATTC+CGG + Chr1:2569702-2569721 MsG0180000183.01.T01:CDS 45.0%
! AGTTGCATGGTTTTCACGGT+GGG + Chr1:2569929-2569948 MsG0180000183.01.T01:CDS 45.0%
! GGGAATTTCACATTTGCTGC+CGG + Chr1:2569862-2569881 MsG0180000183.01.T01:CDS 45.0%
! TACAGATGGCAAGTGGGTTT+GGG + Chr1:2569419-2569438 MsG0180000183.01.T01:intron 45.0%
! TGGATTAGGGGGATTTGTAC+CGG + Chr1:2569822-2569841 MsG0180000183.01.T01:CDS 45.0%
! TTACAGATGGCAAGTGGGTT+TGG + Chr1:2569418-2569437 MsG0180000183.01.T01:intron 45.0%
! TTCACATTTGCTGCCGGATT+TGG + Chr1:2569868-2569887 MsG0180000183.01.T01:CDS 45.0%
!! TGGATTCACAGGTGGATTAG+GGG + Chr1:2569810-2569829 MsG0180000183.01.T01:CDS 45.0%
!! AAAAAATAAATAAAAAATTG+AGG - Chr1:2568486-2568505 None:intergenic 5.0%
AATATCCCTCATCGTCCTGC+CGG + Chr1:2569730-2569749 MsG0180000183.01.T01:CDS 50.0%
ATCCTCTGCACACAGGTCAA+GGG + Chr1:2570025-2570044 MsG0180000183.01.T01:CDS 50.0%
CCCGGAATAGATTTCGACCT+CGG - Chr1:2569706-2569725 None:intergenic 50.0%
CCGAGGTCGAAATCTATTCC+GGG + Chr1:2569703-2569722 MsG0180000183.01.T01:CDS 50.0%
CCGGCAACAACTGCAAGATT+TGG + Chr1:2569841-2569860 MsG0180000183.01.T01:CDS 50.0%
GGTTTGGGGAATCAACAAGC+AGG + Chr1:2569434-2569453 MsG0180000183.01.T01:CDS 50.0%
TACTCGAGTAAGGAGGGCTT+GGG - Chr1:2569465-2569484 None:intergenic 50.0%
TATCCTCTGCACACAGGTCA+AGG + Chr1:2570024-2570043 MsG0180000183.01.T01:CDS 50.0%
TTACTCGAGTAAGGAGGGCT+TGG - Chr1:2569466-2569485 None:intergenic 50.0%
! ACAGATGGCAAGTGGGTTTG+GGG + Chr1:2569420-2569439 MsG0180000183.01.T01:intron 50.0%
! ACATTTGCTGCCGGATTTGG+TGG + Chr1:2569871-2569890 MsG0180000183.01.T01:CDS 50.0%
! CAGTTGCATGGTTTTCACGG+TGG + Chr1:2569928-2569947 MsG0180000183.01.T01:CDS 50.0%
! GTTGCATGGTTTTCACGGTG+GGG + Chr1:2569930-2569949 MsG0180000183.01.T01:CDS 50.0%
! TGCAGTTTCAGGTCTCTGAC+CGG - Chr1:2569752-2569771 None:intergenic 50.0%
!! GAGCTGCTGGTTTCCCTTAT+GGG + Chr1:2569965-2569984 MsG0180000183.01.T01:CDS 50.0%
!! GGATTCACAGGTGGATTAGG+GGG + Chr1:2569811-2569830 MsG0180000183.01.T01:CDS 50.0%
AAACCGGGCATTCTCGTGAC+TGG - Chr1:2569618-2569637 None:intergenic 55.0%
AACCGGGCATTCTCGTGACT+GGG - Chr1:2569617-2569636 None:intergenic 55.0%
ACCAGCAGCTCCACCATACA+TGG - Chr1:2569956-2569975 None:intergenic 55.0%
ACTCGAGTAAGGAGGGCTTG+GGG - Chr1:2569464-2569483 None:intergenic 55.0%
TCCCCCCAATCCACCAAATC+CGG - Chr1:2569884-2569903 None:intergenic 55.0%
TTTCACGGTGGGGCCATGTA+TGG + Chr1:2569940-2569959 MsG0180000183.01.T01:CDS 55.0%
! TCAGGTGGAGGTGCAGTTTC+AGG - Chr1:2569763-2569782 None:intergenic 55.0%
!! CTGCCGGATTTGGTGGATTG+GGG + Chr1:2569878-2569897 MsG0180000183.01.T01:CDS 55.0%
!! GCTGCCGGATTTGGTGGATT+GGG + Chr1:2569877-2569896 MsG0180000183.01.T01:CDS 55.0%
!! GGAGCTGCTGGTTTCCCTTA+TGG + Chr1:2569964-2569983 MsG0180000183.01.T01:CDS 55.0%
!! TGCCGGATTTGGTGGATTGG+GGG + Chr1:2569879-2569898 MsG0180000183.01.T01:CDS 55.0%
!! TGCTGCCGGATTTGGTGGAT+TGG + Chr1:2569876-2569895 MsG0180000183.01.T01:CDS 55.0%
CTCCCAGTCACGAGAATGCC+CGG + Chr1:2569612-2569631 MsG0180000183.01.T01:CDS 60.0%
CTCGAGTAAGGAGGGCTTGG+GGG - Chr1:2569463-2569482 None:intergenic 60.0%
TCTGACCGGCAGGACGATGA+GGG - Chr1:2569738-2569757 None:intergenic 60.0%
TGCCCTTGACCTGTGTGCAG+AGG - Chr1:2570030-2570049 None:intergenic 60.0%
! GCCATGTATGGTGGAGCTGC+TGG + Chr1:2569952-2569971 MsG0180000183.01.T01:CDS 60.0%
! GTTTCAGGTCTCTGACCGGC+AGG - Chr1:2569748-2569767 None:intergenic 60.0%
!! GCCGGATTTGGTGGATTGGG+GGG + Chr1:2569880-2569899 MsG0180000183.01.T01:CDS 60.0%
CACGGTGGGGCCATGTATGG+TGG + Chr1:2569943-2569962 MsG0180000183.01.T01:CDS 65.0%
CTCTGACCGGCAGGACGATG+AGG - Chr1:2569739-2569758 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 2568403 2570409 2568403 ID=MsG0180000183.01;Name=MsG0180000183.01
Chr1 mRNA 2568403 2570409 2568403 ID=MsG0180000183.01.T01;Parent=MsG0180000183.01;Name=MsG0180000183.01.T01;_AED=0.48;_eAED=0.48;_QI=69|1|1|1|0|0.5|2|287|232
Chr1 exon 2569424 2570409 2569424 ID=MsG0180000183.01.T01:exon:6556;Parent=MsG0180000183.01.T01
Chr1 exon 2568403 2568471 2568403 ID=MsG0180000183.01.T01:exon:6555;Parent=MsG0180000183.01.T01
Chr1 five_prime_UTR 2568403 2568471 2568403 ID=MsG0180000183.01.T01:five_prime_utr;Parent=MsG0180000183.01.T01
Chr1 CDS 2569424 2570122 2569424 ID=MsG0180000183.01.T01:cds;Parent=MsG0180000183.01.T01
Chr1 three_prime_UTR 2570123 2570409 2570123 ID=MsG0180000183.01.T01:three_prime_utr;Parent=MsG0180000183.01.T01
Gene Sequence

>MsG0180000183.01.T01

ATGGCAAGTGGGTTTGGGGAATCAACAAGCAGGTCTCCCCCAAGCCCTCCTTACTCGAGTAATAATAATAACAGTGATGCTGGCAATTTTGAATGCAACATTTGTTTTGACTTAGCACAAGATCCTATTATTACTTTGTGTGGTCATCTTTTCTGTTGGCCTTGTCTTTACAAATGGCTTCACTTTCACTCCCAGTCACGAGAATGCCCGGTTTGTAAAGCGATGATTGAGGAGGAGAAATTGGTTCCTTTATATGGGAGAGGAAAGACTTCAACTGATCCGAGGTCGAAATCTATTCCGGGTGTTAATATCCCTCATCGTCCTGCCGGTCAGAGACCTGAAACTGCACCTCCACCTGAATCAAATCCTTTTTCTCAACATGGATTTGGATTCACAGGTGGATTAGGGGGATTTGTACCGGCAACAACTGCAAGATTTGGGAATTTCACATTTGCTGCCGGATTTGGTGGATTGGGGGGATTTATACCATCCTTATTTAACTTTCAGTTGCATGGTTTTCACGGTGGGGCCATGTATGGTGGAGCTGCTGGTTTCCCTTATGGGTTTTCTAATGCATTTCACGGTGGCCATGTTCATAGATATCCTCTGCACACAGGTCAAGGGCAACAAGATTATTATCTGAAGAGGCTGTTGATGTTTGTTATTTTTTGTGTGGTACTTGCTTTACTTTGGCAATAG
Protein sequence

>MsG0180000183.01.T01

MASGFGESTSRSPPSPPYSSNNNNSDAGNFECNICFDLAQDPIITLCGHLFCWPCLYKWLHFHSQSRECPVCKAMIEEEKLVPLYGRGKTSTDPRSKSIPGVNIPHRPAGQRPETAPPPESNPFSQHGFGFTGGLGGFVPATTARFGNFTFAAGFGGLGGFIPSLFNFQLHGFHGGAMYGGAAGFPYGFSNAFHGGHVHRYPLHTGQGQQDYYLKRLLMFVIFCVVLALLWQ*