Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000533.01.T01 | PNY00952.1 | 54.851 | 505 | 154 | 5 | 1 | 443 | 1 | 493 | 2.69E-164 | 486 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000533.01.T01 | G7I877 | 69.163 | 454 | 39 | 3 | 23 | 446 | 9 | 391 | 0.0 | 596 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000533.01.T01 | MTR_1g014060 | 69.163 | 454 | 39 | 3 | 23 | 446 | 9 | 391 | 0.0 | 596 |
| MsG0180000533.01.T01 | MTR_7g066570 | 52.871 | 505 | 157 | 5 | 1 | 444 | 1 | 485 | 1.32e-157 | 458 |
| MsG0180000533.01.T01 | MTR_7g012680 | 51.976 | 506 | 129 | 5 | 1 | 446 | 1 | 452 | 2.65e-152 | 440 |
| MsG0180000533.01.T01 | MTR_7g058710 | 48.330 | 509 | 167 | 12 | 6 | 446 | 4 | 484 | 5.22e-135 | 397 |
| MsG0180000533.01.T01 | MTR_7g066260 | 45.776 | 509 | 168 | 6 | 2 | 445 | 3 | 468 | 7.39e-132 | 389 |
| MsG0180000533.01.T01 | MTR_2g029400 | 49.773 | 440 | 175 | 10 | 1 | 429 | 1 | 405 | 4.07e-129 | 379 |
| MsG0180000533.01.T01 | MTR_7g066340 | 45.129 | 503 | 168 | 8 | 5 | 445 | 3 | 459 | 1.50e-119 | 357 |
| MsG0180000533.01.T01 | MTR_7g066340 | 25.229 | 218 | 141 | 6 | 1 | 210 | 250 | 453 | 1.11e-12 | 70.1 |
| MsG0180000533.01.T01 | MTR_7g066580 | 47.957 | 465 | 185 | 12 | 20 | 445 | 1 | 447 | 1.65e-115 | 346 |
| MsG0180000533.01.T01 | MTR_7g066350 | 44.919 | 492 | 154 | 8 | 10 | 435 | 5 | 445 | 3.83e-115 | 345 |
| MsG0180000533.01.T01 | MTR_7g066400 | 44.694 | 490 | 159 | 10 | 1 | 435 | 1 | 433 | 9.06e-111 | 334 |
| MsG0180000533.01.T01 | MTR_1g041315 | 43.173 | 498 | 161 | 11 | 1 | 423 | 1 | 451 | 5.07e-110 | 333 |
| MsG0180000533.01.T01 | MTR_7g066360 | 43.418 | 509 | 170 | 10 | 1 | 445 | 1 | 455 | 5.66e-102 | 312 |
| MsG0180000533.01.T01 | MTR_7g066430 | 40.121 | 496 | 224 | 8 | 14 | 445 | 4 | 490 | 3.03e-98 | 303 |
| MsG0180000533.01.T01 | MTR_7g066410 | 36.328 | 512 | 231 | 9 | 15 | 440 | 11 | 513 | 2.56e-89 | 281 |
| MsG0180000533.01.T01 | MTR_7g066410 | 26.141 | 241 | 131 | 6 | 2 | 201 | 272 | 506 | 6.99e-16 | 80.1 |
| MsG0180000533.01.T01 | MTR_7g066310 | 43.018 | 444 | 186 | 12 | 11 | 445 | 4 | 389 | 9.63e-87 | 271 |
| MsG0180000533.01.T01 | MTR_7g066310 | 26.794 | 209 | 131 | 7 | 10 | 210 | 189 | 383 | 5.39e-12 | 67.8 |
| MsG0180000533.01.T01 | MTR_7g063920 | 37.155 | 471 | 237 | 10 | 1 | 446 | 1 | 437 | 1.90e-85 | 268 |
| MsG0180000533.01.T01 | MTR_7g066290 | 40.546 | 476 | 203 | 11 | 1 | 439 | 1 | 433 | 1.01e-83 | 264 |
| MsG0180000533.01.T01 | MTR_7g063660 | 35.200 | 500 | 229 | 12 | 13 | 446 | 8 | 478 | 1.59e-80 | 257 |
| MsG0180000533.01.T01 | MTR_7g063880 | 37.045 | 494 | 232 | 16 | 1 | 443 | 1 | 466 | 1.69e-80 | 257 |
| MsG0180000533.01.T01 | MTR_7g062300 | 35.152 | 495 | 239 | 12 | 1 | 437 | 1 | 471 | 2.18e-78 | 251 |
| MsG0180000533.01.T01 | MTR_7g063900 | 38.540 | 493 | 230 | 15 | 14 | 446 | 10 | 489 | 3.14e-78 | 251 |
| MsG0180000533.01.T01 | MTR_7g063840 | 34.825 | 514 | 227 | 14 | 1 | 446 | 1 | 474 | 9.11e-78 | 249 |
| MsG0180000533.01.T01 | MTR_7g062280 | 34.825 | 514 | 237 | 12 | 6 | 446 | 4 | 492 | 5.75e-77 | 248 |
| MsG0180000533.01.T01 | MTR_7g063820 | 37.349 | 498 | 238 | 17 | 8 | 446 | 3 | 485 | 2.27e-76 | 246 |
| MsG0180000533.01.T01 | MTR_7g058700 | 46.784 | 342 | 106 | 9 | 26 | 306 | 531 | 857 | 1.94e-74 | 251 |
| MsG0180000533.01.T01 | MTR_7g058700 | 55.696 | 79 | 35 | 0 | 368 | 446 | 850 | 928 | 1.34e-19 | 92.4 |
| MsG0180000533.01.T01 | MTR_7g063890 | 37.097 | 496 | 232 | 13 | 13 | 445 | 26 | 504 | 1.60e-72 | 237 |
| MsG0180000533.01.T01 | MTR_7g066480 | 37.391 | 460 | 206 | 15 | 13 | 441 | 15 | 423 | 5.75e-70 | 228 |
| MsG0180000533.01.T01 | MTR_3g007740 | 34.221 | 488 | 209 | 11 | 13 | 440 | 8 | 443 | 2.36e-69 | 227 |
| MsG0180000533.01.T01 | MTR_7g063670 | 35.141 | 498 | 210 | 13 | 10 | 446 | 13 | 458 | 6.36e-67 | 221 |
| MsG0180000533.01.T01 | MTR_7g063830 | 33.537 | 492 | 212 | 16 | 8 | 440 | 4 | 439 | 1.57e-64 | 214 |
| MsG0180000533.01.T01 | MTR_4g019310 | 31.969 | 513 | 244 | 17 | 1 | 435 | 1 | 486 | 3.46e-60 | 204 |
| MsG0180000533.01.T01 | MTR_7g066490 | 39.157 | 332 | 135 | 7 | 14 | 291 | 16 | 334 | 2.74e-56 | 192 |
| MsG0180000533.01.T01 | MTR_7g066490 | 58.333 | 72 | 30 | 0 | 370 | 441 | 338 | 409 | 4.99e-18 | 86.3 |
| MsG0180000533.01.T01 | MTR_7g066490 | 25.405 | 185 | 103 | 4 | 17 | 201 | 252 | 401 | 1.10e-12 | 69.7 |
| MsG0180000533.01.T01 | MTR_3g065200 | 32.009 | 453 | 223 | 10 | 12 | 425 | 1 | 407 | 1.96e-54 | 188 |
| MsG0180000533.01.T01 | MTR_7g066680 | 37.618 | 319 | 111 | 5 | 184 | 435 | 37 | 334 | 1.10e-53 | 183 |
| MsG0180000533.01.T01 | MTR_4g017610 | 30.020 | 503 | 256 | 14 | 1 | 435 | 1 | 475 | 2.06e-50 | 178 |
| MsG0180000533.01.T01 | MTR_4g017830 | 30.740 | 527 | 263 | 19 | 1 | 445 | 1 | 507 | 2.73e-49 | 176 |
| MsG0180000533.01.T01 | MTR_7g066280 | 32.653 | 441 | 164 | 11 | 93 | 445 | 1 | 396 | 5.06e-49 | 172 |
| MsG0180000533.01.T01 | MTR_4g019340 | 31.048 | 496 | 226 | 14 | 15 | 427 | 39 | 501 | 1.40e-48 | 174 |
| MsG0180000533.01.T01 | MTR_4g017440 | 29.310 | 522 | 278 | 14 | 1 | 445 | 1 | 508 | 4.27e-48 | 172 |
| MsG0180000533.01.T01 | MTR_4g017470 | 30.297 | 505 | 263 | 14 | 1 | 445 | 1 | 476 | 3.64e-47 | 169 |
| MsG0180000533.01.T01 | MTR_4g017410 | 29.730 | 518 | 254 | 18 | 8 | 445 | 3 | 490 | 3.41e-43 | 159 |
| MsG0180000533.01.T01 | MTR_7g063720 | 37.209 | 215 | 102 | 5 | 232 | 446 | 52 | 233 | 2.74e-34 | 128 |
| MsG0180000533.01.T01 | MTR_7g063720 | 28.293 | 205 | 123 | 6 | 14 | 201 | 23 | 220 | 1.35e-17 | 82.4 |
| MsG0180000533.01.T01 | MTR_2g067310 | 44.755 | 143 | 69 | 2 | 25 | 167 | 58 | 190 | 3.84e-28 | 112 |
| MsG0180000533.01.T01 | MTR_7g062270 | 37.968 | 187 | 79 | 5 | 1 | 156 | 1 | 181 | 3.88e-28 | 115 |
| MsG0180000533.01.T01 | MTR_4g017550 | 28.842 | 475 | 235 | 21 | 43 | 437 | 20 | 471 | 8.60e-27 | 112 |
| MsG0180000533.01.T01 | MTR_7g063690 | 36.321 | 212 | 99 | 4 | 1 | 211 | 1 | 177 | 1.32e-26 | 107 |
| MsG0180000533.01.T01 | MTR_7g063850 | 30.619 | 307 | 175 | 11 | 156 | 445 | 3 | 288 | 1.08e-24 | 103 |
| MsG0180000533.01.T01 | MTR_7g066390 | 60.976 | 82 | 32 | 0 | 364 | 445 | 47 | 128 | 6.17e-23 | 95.1 |
| MsG0180000533.01.T01 | MTR_1g115440 | 28.085 | 235 | 124 | 4 | 13 | 207 | 10 | 239 | 4.71e-22 | 95.1 |
| MsG0180000533.01.T01 | MTR_1g115440 | 28.293 | 205 | 131 | 6 | 232 | 432 | 40 | 232 | 7.60e-12 | 65.5 |
| MsG0180000533.01.T01 | MTR_8g445860 | 31.169 | 231 | 115 | 8 | 14 | 206 | 13 | 237 | 1.51e-21 | 93.6 |
| MsG0180000533.01.T01 | MTR_0150s0010 | 29.167 | 240 | 125 | 5 | 8 | 207 | 4 | 238 | 5.33e-21 | 92.0 |
| MsG0180000533.01.T01 | MTR_8g023240 | 24.145 | 468 | 284 | 18 | 15 | 433 | 38 | 483 | 2.26e-20 | 94.0 |
| MsG0180000533.01.T01 | MTR_4g019240 | 40.909 | 110 | 56 | 3 | 1 | 102 | 1 | 109 | 7.68e-18 | 81.6 |
| MsG0180000533.01.T01 | MTR_7g100420 | 28.319 | 339 | 183 | 8 | 131 | 439 | 89 | 397 | 6.26e-17 | 82.8 |
| MsG0180000533.01.T01 | MTR_7g100420 | 73.810 | 42 | 11 | 0 | 15 | 56 | 40 | 81 | 5.72e-13 | 70.9 |
| MsG0180000533.01.T01 | MTR_1g115450 | 38.824 | 85 | 47 | 1 | 14 | 98 | 7 | 86 | 1.67e-14 | 70.9 |
| MsG0180000533.01.T01 | MTR_7g063760 | 46.575 | 73 | 38 | 1 | 375 | 446 | 2 | 74 | 1.10e-13 | 66.2 |
| MsG0180000533.01.T01 | MTR_1g115455 | 38.824 | 85 | 47 | 1 | 14 | 98 | 9 | 88 | 5.05e-13 | 68.9 |
| MsG0180000533.01.T01 | MTR_1g115470 | 36.364 | 88 | 51 | 1 | 14 | 101 | 6 | 88 | 2.53e-11 | 65.9 |
| MsG0180000533.01.T01 | MTR_0150s0030 | 32.673 | 101 | 59 | 3 | 8 | 104 | 2 | 97 | 3.92e-11 | 62.8 |
| MsG0180000533.01.T01 | MTR_8g445920 | 32.584 | 89 | 55 | 1 | 8 | 96 | 4 | 87 | 7.91e-11 | 59.3 |
| MsG0180000533.01.T01 | MTR_8g044890 | 32.584 | 89 | 55 | 1 | 8 | 96 | 4 | 87 | 7.91e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000533.01.T01 | AT5G01120 | 27.711 | 498 | 261 | 14 | 15 | 435 | 10 | 485 | 1.82e-40 | 151 |
| MsG0180000533.01.T01 | AT3G09140 | 26.938 | 516 | 258 | 12 | 14 | 440 | 9 | 494 | 6.45e-36 | 139 |
| MsG0180000533.01.T01 | AT3G09110 | 28.571 | 315 | 156 | 4 | 14 | 264 | 9 | 318 | 5.89e-35 | 133 |
| MsG0180000533.01.T01 | AT5G01150 | 27.033 | 492 | 272 | 12 | 14 | 439 | 9 | 479 | 7.03e-35 | 136 |
| MsG0180000533.01.T01 | AT5G01130 | 27.443 | 481 | 249 | 11 | 14 | 439 | 9 | 444 | 1.85e-33 | 131 |
| MsG0180000533.01.T01 | AT5G01130 | 30.288 | 208 | 114 | 7 | 15 | 205 | 253 | 446 | 4.26e-14 | 74.3 |
| MsG0180000533.01.T01 | AT3G09140 | 26.374 | 546 | 251 | 15 | 14 | 440 | 9 | 522 | 6.00e-32 | 128 |
| MsG0180000533.01.T01 | AT5G43240 | 26.016 | 492 | 281 | 15 | 15 | 435 | 10 | 489 | 1.40e-31 | 127 |
| MsG0180000533.01.T01 | AT5G43240 | 26.016 | 492 | 281 | 15 | 15 | 435 | 10 | 489 | 1.40e-31 | 127 |
| MsG0180000533.01.T01 | AT5G43240 | 26.016 | 492 | 281 | 15 | 15 | 435 | 10 | 489 | 1.40e-31 | 127 |
| MsG0180000533.01.T01 | AT3G09120 | 31.061 | 264 | 144 | 4 | 13 | 239 | 5 | 267 | 1.84e-30 | 120 |
| MsG0180000533.01.T01 | AT5G01140 | 24.490 | 490 | 259 | 12 | 14 | 439 | 9 | 451 | 3.54e-27 | 114 |
| MsG0180000533.01.T01 | AT5G01140 | 26.577 | 222 | 125 | 6 | 15 | 206 | 241 | 454 | 7.81e-13 | 70.5 |
| MsG0180000533.01.T01 | AT5G37320 | 36.275 | 102 | 64 | 1 | 15 | 115 | 10 | 111 | 4.59e-15 | 77.4 |
Find 82 sgRNAs with CRISPR-Local
Find 103 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGCGTTAGTTGGTAATAAT+TGG | 0.161706 | 1:+7491458 | None:intergenic |
| CCAACCAATTTGAGCTTAAA+TGG | 0.177100 | 1:+7491313 | None:intergenic |
| GAGGAAATAATCATCAAAAT+TGG | 0.250442 | 1:-7491118 | MsG0180000533.01.T01:CDS |
| AATATTGAAGCAGTGGAATT+TGG | 0.254893 | 1:-7492413 | MsG0180000533.01.T01:CDS |
| CCTCCAGAACATTTGAGTTT+AGG | 0.260762 | 1:-7492133 | MsG0180000533.01.T01:CDS |
| TAAAATTCCATGGAGTTTCT+TGG | 0.280411 | 1:+7492305 | None:intergenic |
| CTGTGAGATTAAATATCTTC+TGG | 0.304715 | 1:+7491537 | None:intergenic |
| TTTCCTAAACTCAAATGTTC+TGG | 0.304784 | 1:+7492130 | None:intergenic |
| TAAAGTTCCTCTGTCTGATT+TGG | 0.321917 | 1:-7491140 | MsG0180000533.01.T01:CDS |
| TGTACCATTTAAGCTCAAAT+TGG | 0.323929 | 1:-7491317 | MsG0180000533.01.T01:CDS |
| TTAAATGGTACAAACTTATC+AGG | 0.348518 | 1:+7491328 | None:intergenic |
| CAATGTTCAGTTTCATCTGC+TGG | 0.399362 | 1:+7492279 | None:intergenic |
| ATCTTGATCAACAATATCTA+TGG | 0.406573 | 1:-7492358 | MsG0180000533.01.T01:CDS |
| TTAAGATTAAATCTGTCAAA+GGG | 0.410150 | 1:+7491831 | None:intergenic |
| TTCAAATGATGCTGAAACAT+AGG | 0.414974 | 1:+7491282 | None:intergenic |
| ACAACTGTTGCCAAAACATT+AGG | 0.426351 | 1:+7492052 | None:intergenic |
| TCAACGTCTTCTTTGACAAA+TGG | 0.430286 | 1:-7490960 | MsG0180000533.01.T01:CDS |
| CAATCATTTCTCCACCTTGA+CGG | 0.431842 | 1:+7490917 | None:intergenic |
| AGTTCCTCTGTCTGATTTGG+AGG | 0.440102 | 1:-7491137 | MsG0180000533.01.T01:CDS |
| GCTTAGTTAACTTCTCTTTG+AGG | 0.442124 | 1:+7491512 | None:intergenic |
| CACCTTACCTTTGGGAGCAG+TGG | 0.444063 | 1:-7491632 | MsG0180000533.01.T01:CDS |
| GAACATTTGAGTTTAGGAAA+TGG | 0.449684 | 1:-7492127 | MsG0180000533.01.T01:CDS |
| TTCAACGGATTGAGTTTCAT+TGG | 0.452229 | 1:+7492586 | None:intergenic |
| TCTGCCACCATGTATGTTGA+TGG | 0.452833 | 1:+7491220 | None:intergenic |
| CAAAGTTAGCTTGAGAATCA+TGG | 0.455051 | 1:-7492555 | MsG0180000533.01.T01:CDS |
| TGTGAGATTAAATATCTTCT+GGG | 0.455073 | 1:+7491538 | None:intergenic |
| AATCATCAAAATTGGTAAGA+AGG | 0.458294 | 1:-7491110 | MsG0180000533.01.T01:CDS |
| ATGCTATTGTGGTAGAGTAC+TGG | 0.479902 | 1:-7492167 | MsG0180000533.01.T01:intron |
| AGAGTTCTATATGCTGAGGC+TGG | 0.480045 | 1:-7492515 | MsG0180000533.01.T01:CDS |
| TGCAGATCTAACAAAAGAAA+AGG | 0.486365 | 1:-7491377 | MsG0180000533.01.T01:CDS |
| GGAAAGGATGCGGCGTTAGT+TGG | 0.487538 | 1:+7491448 | None:intergenic |
| AGCAGCATTTCTTTGCATGC+CGG | 0.487860 | 1:+7492332 | None:intergenic |
| TGGAGAGAAACAATCTGATA+CGG | 0.489375 | 1:-7491753 | MsG0180000533.01.T01:CDS |
| AGTTTCTTAACATTACCTCT+AGG | 0.491345 | 1:-7492467 | MsG0180000533.01.T01:CDS |
| TTTAAGATTAAATCTGTCAA+AGG | 0.492459 | 1:+7491830 | None:intergenic |
| CCATTTAAGCTCAAATTGGT+TGG | 0.500263 | 1:-7491313 | MsG0180000533.01.T01:CDS |
| GAATCATGGTGGACAAAGAA+AGG | 0.504639 | 1:-7492541 | MsG0180000533.01.T01:CDS |
| TATGTATGACAATAATATGC+TGG | 0.519223 | 1:+7491427 | None:intergenic |
| GCAGTGGTGCATATGCTTCA+AGG | 0.520737 | 1:-7491616 | MsG0180000533.01.T01:CDS |
| AACTTTGTCAACTTGTTCAA+CGG | 0.522676 | 1:+7492571 | None:intergenic |
| TGGATTCTACCACGGAAATG+TGG | 0.528178 | 1:+7491478 | None:intergenic |
| GAAGTCAAATATTGAAGCAG+TGG | 0.536513 | 1:-7492420 | MsG0180000533.01.T01:CDS |
| GTTTCTTAACATTACCTCTA+GGG | 0.537673 | 1:-7492466 | MsG0180000533.01.T01:CDS |
| CTGCTGGAAATAAAATTCCA+TGG | 0.541132 | 1:+7492295 | None:intergenic |
| CACCACTGCTCCCAAAGGTA+AGG | 0.543542 | 1:+7491630 | None:intergenic |
| TCTATATGCTGAGGCTGGGA+AGG | 0.548508 | 1:-7492510 | MsG0180000533.01.T01:CDS |
| CTCATTGTATCGAGGTGTGT+CGG | 0.559520 | 1:-7492381 | MsG0180000533.01.T01:CDS |
| GGATTCTACCACGGAAATGT+GGG | 0.559747 | 1:+7491479 | None:intergenic |
| CAGAAGATATTTAATCTCAC+AGG | 0.560764 | 1:-7491536 | MsG0180000533.01.T01:CDS |
| GAGTTCTATATGCTGAGGCT+GGG | 0.563406 | 1:-7492514 | MsG0180000533.01.T01:CDS |
| GTACATCTACATCAGAAGCT+TGG | 0.564486 | 1:-7492031 | MsG0180000533.01.T01:CDS |
| TGTGGAAATAAGCAAGAAAG+AGG | 0.566925 | 1:-7491975 | MsG0180000533.01.T01:intron |
| GGAGAGAAACAATCTGATAC+GGG | 0.574172 | 1:-7491752 | MsG0180000533.01.T01:CDS |
| CCTAAACTCAAATGTTCTGG+AGG | 0.578393 | 1:+7492133 | None:intergenic |
| GCTGCAGCCAAGAAACTCCA+TGG | 0.582763 | 1:-7492312 | MsG0180000533.01.T01:CDS |
| ATAGTAGATATTGACGACAT+TGG | 0.589636 | 1:+7491181 | None:intergenic |
| TGATAATTTATACAAAAGCA+TGG | 0.590842 | 1:-7491572 | MsG0180000533.01.T01:CDS |
| ATAATATGCTGGAAAGGATG+CGG | 0.601036 | 1:+7491438 | None:intergenic |
| ATGACAATAATATGCTGGAA+AGG | 0.607141 | 1:+7491432 | None:intergenic |
| GTAATAATTGGATTCTACCA+CGG | 0.609750 | 1:+7491470 | None:intergenic |
| CATCAAAATTGGTAAGAAGG+AGG | 0.613460 | 1:-7491107 | MsG0180000533.01.T01:intron |
| TAGAGGACCATCAACATACA+TGG | 0.625050 | 1:-7491227 | MsG0180000533.01.T01:CDS |
| TCAACAATATCTATGGTCAC+CGG | 0.632396 | 1:-7492351 | MsG0180000533.01.T01:CDS |
| ATACTATAATAGCGATAGTG+AGG | 0.636015 | 1:-7491407 | MsG0180000533.01.T01:CDS |
| CAGTGTGCCCCACATTTCCG+TGG | 0.638161 | 1:-7491487 | MsG0180000533.01.T01:CDS |
| GAACATTGATGACACACCGA+TGG | 0.639013 | 1:-7492264 | MsG0180000533.01.T01:CDS |
| TAAGATTAAATCTGTCAAAG+GGG | 0.639105 | 1:+7491832 | None:intergenic |
| GAACAGAGTTCTATATGCTG+AGG | 0.646830 | 1:-7492519 | MsG0180000533.01.T01:CDS |
| ATATGCACCACTGCTCCCAA+AGG | 0.647683 | 1:+7491625 | None:intergenic |
| GCAGTTCATAACAACCGTCA+AGG | 0.650677 | 1:-7490931 | MsG0180000533.01.T01:CDS |
| AGAAACAATCTGATACGGGT+AGG | 0.651343 | 1:-7491748 | MsG0180000533.01.T01:intron |
| TGCTATCGTTGAACAAACTG+TGG | 0.652160 | 1:-7491993 | MsG0180000533.01.T01:CDS |
| CTACAGAAGAAGTACTCCAT+CGG | 0.654069 | 1:+7492248 | None:intergenic |
| ATTTCCTCCAAATCAGACAG+AGG | 0.658378 | 1:+7491133 | None:intergenic |
| GCGGCATCGTCATATGCTAG+AGG | 0.665739 | 1:-7491244 | MsG0180000533.01.T01:CDS |
| GATTCTACCACGGAAATGTG+GGG | 0.668083 | 1:+7491480 | None:intergenic |
| AGCATTAGCTCATTGTATCG+AGG | 0.679958 | 1:-7492389 | MsG0180000533.01.T01:CDS |
| GTTCATAACAACCGTCAAGG+TGG | 0.684380 | 1:-7490928 | MsG0180000533.01.T01:CDS |
| CGGAAATGTGGGGCACACTG+AGG | 0.686859 | 1:+7491490 | None:intergenic |
| AGTTAGCTTGAGAATCATGG+TGG | 0.711016 | 1:-7492552 | MsG0180000533.01.T01:CDS |
| AGTCGTGCAATAGTCCCTAG+AGG | 0.729079 | 1:+7492452 | None:intergenic |
| AGGACCATCAACATACATGG+TGG | 0.750812 | 1:-7491224 | MsG0180000533.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAATATGAATTCGGAAA+TGG | - | Chr1:7491740-7491759 | MsG0180000533.01.T01:intron | 20.0% |
| !! | CAAAGCAAAAAATATGAATT+CGG | - | Chr1:7491734-7491753 | MsG0180000533.01.T01:intron | 20.0% |
| !! | GCAAATTAAAATAAATCACA+AGG | + | Chr1:7492468-7492487 | None:intergenic | 20.0% |
| !! | TGATAATTTATACAAAAGCA+TGG | - | Chr1:7491941-7491960 | MsG0180000533.01.T01:intron | 20.0% |
| !! | TTAAGATTAAATCTGTCAAA+GGG | + | Chr1:7491685-7491704 | None:intergenic | 20.0% |
| !!! | TTTAAGATTAAATCTGTCAA+AGG | + | Chr1:7491686-7491705 | None:intergenic | 20.0% |
| ! | AATCATCAAAATTGGTAAGA+AGG | - | Chr1:7492403-7492422 | MsG0180000533.01.T01:CDS | 25.0% |
| ! | ATCTTGATCAACAATATCTA+TGG | - | Chr1:7491155-7491174 | MsG0180000533.01.T01:CDS | 25.0% |
| ! | ATTACAGATTATATAGAAGC+CGG | + | Chr1:7491572-7491591 | None:intergenic | 25.0% |
| ! | GAGGAAATAATCATCAAAAT+TGG | - | Chr1:7492395-7492414 | MsG0180000533.01.T01:CDS | 25.0% |
| ! | TAAGATTAAATCTGTCAAAG+GGG | + | Chr1:7491684-7491703 | None:intergenic | 25.0% |
| ! | TATGTATGACAATAATATGC+TGG | + | Chr1:7492089-7492108 | None:intergenic | 25.0% |
| ! | TGTGAGATTAAATATCTTCT+GGG | + | Chr1:7491978-7491997 | None:intergenic | 25.0% |
| ! | TTAAATGGTACAAACTTATC+AGG | + | Chr1:7492188-7492207 | None:intergenic | 25.0% |
| !!! | CGAATTCATATTTTTTGCTT+TGG | + | Chr1:7491736-7491755 | None:intergenic | 25.0% |
| AATATTGAAGCAGTGGAATT+TGG | - | Chr1:7491100-7491119 | MsG0180000533.01.T01:intron | 30.0% | |
| ACAAGGCATAATATAACACT+TGG | + | Chr1:7492451-7492470 | None:intergenic | 30.0% | |
| AGGAATCAAAAATGCTATTG+TGG | - | Chr1:7491335-7491354 | MsG0180000533.01.T01:CDS | 30.0% | |
| AGTGTAATAGAAGAGAAAGT+GGG | - | Chr1:7491296-7491315 | MsG0180000533.01.T01:CDS | 30.0% | |
| AGTTTCTTAACATTACCTCT+AGG | - | Chr1:7491046-7491065 | MsG0180000533.01.T01:intron | 30.0% | |
| ATACTATAATAGCGATAGTG+AGG | - | Chr1:7492106-7492125 | MsG0180000533.01.T01:CDS | 30.0% | |
| ATAGTAGATATTGACGACAT+TGG | + | Chr1:7492335-7492354 | None:intergenic | 30.0% | |
| ATGACAATAATATGCTGGAA+AGG | + | Chr1:7492084-7492103 | None:intergenic | 30.0% | |
| CAGAAGATATTTAATCTCAC+AGG | - | Chr1:7491977-7491996 | MsG0180000533.01.T01:CDS | 30.0% | |
| CTGTGAGATTAAATATCTTC+TGG | + | Chr1:7491979-7491998 | None:intergenic | 30.0% | |
| GTAATAATTGGATTCTACCA+CGG | + | Chr1:7492046-7492065 | None:intergenic | 30.0% | |
| GTTTCTTAACATTACCTCTA+GGG | - | Chr1:7491047-7491066 | MsG0180000533.01.T01:intron | 30.0% | |
| TAAAATTCCATGGAGTTTCT+TGG | + | Chr1:7491211-7491230 | None:intergenic | 30.0% | |
| TGCAGATCTAACAAAAGAAA+AGG | - | Chr1:7492136-7492155 | MsG0180000533.01.T01:CDS | 30.0% | |
| TGTACCATTTAAGCTCAAAT+TGG | - | Chr1:7492196-7492215 | MsG0180000533.01.T01:intron | 30.0% | |
| TTCAAATGATGCTGAAACAT+AGG | + | Chr1:7492234-7492253 | None:intergenic | 30.0% | |
| TTTCCTAAACTCAAATGTTC+TGG | + | Chr1:7491386-7491405 | None:intergenic | 30.0% | |
| ! | AACTTTGTCAACTTGTTCAA+CGG | + | Chr1:7490945-7490964 | None:intergenic | 30.0% |
| ! | GAACATTTGAGTTTAGGAAA+TGG | - | Chr1:7491386-7491405 | MsG0180000533.01.T01:CDS | 30.0% |
| ! | TTTTGTGATGCCTAATGTTT+TGG | - | Chr1:7491451-7491470 | MsG0180000533.01.T01:CDS | 30.0% |
| !! | ATTTCATCCTTTGCTTTTGT+AGG | - | Chr1:7491622-7491641 | MsG0180000533.01.T01:CDS | 30.0% |
| ACAACTGTTGCCAAAACATT+AGG | + | Chr1:7491464-7491483 | None:intergenic | 35.0% | |
| AGTACTTCTTCTGTAGTGAT+TGG | - | Chr1:7491272-7491291 | MsG0180000533.01.T01:CDS | 35.0% | |
| ATAATATGCTGGAAAGGATG+CGG | + | Chr1:7492078-7492097 | None:intergenic | 35.0% | |
| CATCAAAATTGGTAAGAAGG+AGG | - | Chr1:7492406-7492425 | MsG0180000533.01.T01:CDS | 35.0% | |
| CCAACCAATTTGAGCTTAAA+TGG | + | Chr1:7492203-7492222 | None:intergenic | 35.0% | |
| CCATTTAAGCTCAAATTGGT+TGG | - | Chr1:7492200-7492219 | MsG0180000533.01.T01:intron | 35.0% | |
| CTGCTGGAAATAAAATTCCA+TGG | + | Chr1:7491221-7491240 | None:intergenic | 35.0% | |
| GAAGTCAAATATTGAAGCAG+TGG | - | Chr1:7491093-7491112 | MsG0180000533.01.T01:intron | 35.0% | |
| GAGTGTAATAGAAGAGAAAG+TGG | - | Chr1:7491295-7491314 | MsG0180000533.01.T01:CDS | 35.0% | |
| GCTTAGTTAACTTCTCTTTG+AGG | + | Chr1:7492004-7492023 | None:intergenic | 35.0% | |
| GTACATGGTTGTGAAACTAA+TGG | - | Chr1:7491787-7491806 | MsG0180000533.01.T01:CDS | 35.0% | |
| GTACTTCTTCTGTAGTGATT+GGG | - | Chr1:7491273-7491292 | MsG0180000533.01.T01:CDS | 35.0% | |
| TAAAGTTCCTCTGTCTGATT+TGG | - | Chr1:7492373-7492392 | MsG0180000533.01.T01:CDS | 35.0% | |
| TCAACAATATCTATGGTCAC+CGG | - | Chr1:7491162-7491181 | MsG0180000533.01.T01:CDS | 35.0% | |
| TCAACGTCTTCTTTGACAAA+TGG | - | Chr1:7492553-7492572 | MsG0180000533.01.T01:CDS | 35.0% | |
| TCAACTACCTACAAAAGCAA+AGG | + | Chr1:7491632-7491651 | None:intergenic | 35.0% | |
| TGAAAAATTCGCATGCATAG+CGG | - | Chr1:7492250-7492269 | MsG0180000533.01.T01:intron | 35.0% | |
| TGGAGAGAAACAATCTGATA+CGG | - | Chr1:7491760-7491779 | MsG0180000533.01.T01:CDS | 35.0% | |
| TGTGGAAATAAGCAAGAAAG+AGG | - | Chr1:7491538-7491557 | MsG0180000533.01.T01:CDS | 35.0% | |
| ! | AGTTTTCTCACCTTACCTTT+GGG | - | Chr1:7491873-7491892 | MsG0180000533.01.T01:intron | 35.0% |
| ! | CAAAGTTAGCTTGAGAATCA+TGG | - | Chr1:7490958-7490977 | MsG0180000533.01.T01:CDS | 35.0% |
| !! | CAAGAAAGAGGTATGTTTTC+CGG | - | Chr1:7491550-7491569 | MsG0180000533.01.T01:CDS | 35.0% |
| !! | TTCAACGGATTGAGTTTCAT+TGG | + | Chr1:7490930-7490949 | None:intergenic | 35.0% |
| AGAAACAATCTGATACGGGT+AGG | - | Chr1:7491765-7491784 | MsG0180000533.01.T01:CDS | 40.0% | |
| AGCATTAGCTCATTGTATCG+AGG | - | Chr1:7491124-7491143 | MsG0180000533.01.T01:CDS | 40.0% | |
| AGGCATCACAAAAAGATCGT+CGG | + | Chr1:7491444-7491463 | None:intergenic | 40.0% | |
| ATGCTATTGTGGTAGAGTAC+TGG | - | Chr1:7491346-7491365 | MsG0180000533.01.T01:CDS | 40.0% | |
| ATTTCCTCCAAATCAGACAG+AGG | + | Chr1:7492383-7492402 | None:intergenic | 40.0% | |
| CAATGTTCAGTTTCATCTGC+TGG | + | Chr1:7491237-7491256 | None:intergenic | 40.0% | |
| CCTAAACTCAAATGTTCTGG+AGG | + | Chr1:7491383-7491402 | None:intergenic | 40.0% | |
| GAACAGAGTTCTATATGCTG+AGG | - | Chr1:7490994-7491013 | MsG0180000533.01.T01:intron | 40.0% | |
| GGAGAGAAACAATCTGATAC+GGG | - | Chr1:7491761-7491780 | MsG0180000533.01.T01:CDS | 40.0% | |
| GTACATCTACATCAGAAGCT+TGG | - | Chr1:7491482-7491501 | MsG0180000533.01.T01:CDS | 40.0% | |
| TAGAGGACCATCAACATACA+TGG | - | Chr1:7492286-7492305 | MsG0180000533.01.T01:CDS | 40.0% | |
| TGCTATCGTTGAACAAACTG+TGG | - | Chr1:7491520-7491539 | MsG0180000533.01.T01:CDS | 40.0% | |
| ! | AGTTAGCTTGAGAATCATGG+TGG | - | Chr1:7490961-7490980 | MsG0180000533.01.T01:CDS | 40.0% |
| ! | CAGTTTTCTCACCTTACCTT+TGG | - | Chr1:7491872-7491891 | MsG0180000533.01.T01:CDS | 40.0% |
| ! | CCTCCAGAACATTTGAGTTT+AGG | - | Chr1:7491380-7491399 | MsG0180000533.01.T01:CDS | 40.0% |
| !! | CGGCGTTAGTTGGTAATAAT+TGG | + | Chr1:7492058-7492077 | None:intergenic | 40.0% |
| !! | CTACAGAAGAAGTACTCCAT+CGG | + | Chr1:7491268-7491287 | None:intergenic | 40.0% |
| !! | GAATCATGGTGGACAAAGAA+AGG | - | Chr1:7490972-7490991 | MsG0180000533.01.T01:CDS | 40.0% |
| AGAGTTCTATATGCTGAGGC+TGG | - | Chr1:7490998-7491017 | MsG0180000533.01.T01:intron | 45.0% | |
| AGCAGCATTTCTTTGCATGC+CGG | + | Chr1:7491184-7491203 | None:intergenic | 45.0% | |
| AGGACCATCAACATACATGG+TGG | - | Chr1:7492289-7492308 | MsG0180000533.01.T01:CDS | 45.0% | |
| ATCTGATACGGGTAGGTACA+TGG | - | Chr1:7491772-7491791 | MsG0180000533.01.T01:CDS | 45.0% | |
| CTCATTGTATCGAGGTGTGT+CGG | - | Chr1:7491132-7491151 | MsG0180000533.01.T01:CDS | 45.0% | |
| GAGTTCTATATGCTGAGGCT+GGG | - | Chr1:7490999-7491018 | MsG0180000533.01.T01:intron | 45.0% | |
| GATTCTACCACGGAAATGTG+GGG | + | Chr1:7492036-7492055 | None:intergenic | 45.0% | |
| GCAGTTCATAACAACCGTCA+AGG | - | Chr1:7492582-7492601 | MsG0180000533.01.T01:CDS | 45.0% | |
| GGATTCTACCACGGAAATGT+GGG | + | Chr1:7492037-7492056 | None:intergenic | 45.0% | |
| GTTCATAACAACCGTCAAGG+TGG | - | Chr1:7492585-7492604 | MsG0180000533.01.T01:CDS | 45.0% | |
| TCTGCCACCATGTATGTTGA+TGG | + | Chr1:7492296-7492315 | None:intergenic | 45.0% | |
| TGGATTCTACCACGGAAATG+TGG | + | Chr1:7492038-7492057 | None:intergenic | 45.0% | |
| ! | GAACATTGATGACACACCGA+TGG | - | Chr1:7491249-7491268 | MsG0180000533.01.T01:CDS | 45.0% |
| !! | AGTTCCTCTGTCTGATTTGG+AGG | - | Chr1:7492376-7492395 | MsG0180000533.01.T01:CDS | 45.0% |
| !! | TGGGAATCTTTTGAGCACTG+AGG | - | Chr1:7491315-7491334 | MsG0180000533.01.T01:CDS | 45.0% |
| AGTCGTGCAATAGTCCCTAG+AGG | + | Chr1:7491064-7491083 | None:intergenic | 50.0% | |
| ATATGCACCACTGCTCCCAA+AGG | + | Chr1:7491891-7491910 | None:intergenic | 50.0% | |
| GCAGTGGTGCATATGCTTCA+AGG | - | Chr1:7491897-7491916 | MsG0180000533.01.T01:intron | 50.0% | |
| TCTATATGCTGAGGCTGGGA+AGG | - | Chr1:7491003-7491022 | MsG0180000533.01.T01:intron | 50.0% | |
| CACCACTGCTCCCAAAGGTA+AGG | + | Chr1:7491886-7491905 | None:intergenic | 55.0% | |
| GCGGCATCGTCATATGCTAG+AGG | - | Chr1:7492269-7492288 | MsG0180000533.01.T01:CDS | 55.0% | |
| GCTGCAGCCAAGAAACTCCA+TGG | - | Chr1:7491201-7491220 | MsG0180000533.01.T01:CDS | 55.0% | |
| GGAAAGGATGCGGCGTTAGT+TGG | + | Chr1:7492068-7492087 | None:intergenic | 55.0% | |
| ! | CACCTTACCTTTGGGAGCAG+TGG | - | Chr1:7491881-7491900 | MsG0180000533.01.T01:intron | 55.0% |
| CAGTGTGCCCCACATTTCCG+TGG | - | Chr1:7492026-7492045 | MsG0180000533.01.T01:CDS | 60.0% | |
| CGGAAATGTGGGGCACACTG+AGG | + | Chr1:7492026-7492045 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 7490920 | 7492615 | 7490920 | ID=MsG0180000533.01;Name=MsG0180000533.01 |
| Chr1 | mRNA | 7490920 | 7492615 | 7490920 | ID=MsG0180000533.01.T01;Parent=MsG0180000533.01;Name=MsG0180000533.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|5|0|446 |
| Chr1 | exon | 7492263 | 7492615 | 7492263 | ID=MsG0180000533.01.T01:exon:27601;Parent=MsG0180000533.01.T01 |
| Chr1 | exon | 7491976 | 7492174 | 7491976 | ID=MsG0180000533.01.T01:exon:27602;Parent=MsG0180000533.01.T01 |
| Chr1 | exon | 7491749 | 7491891 | 7491749 | ID=MsG0180000533.01.T01:exon:27603;Parent=MsG0180000533.01.T01 |
| Chr1 | exon | 7491108 | 7491660 | 7491108 | ID=MsG0180000533.01.T01:exon:27604;Parent=MsG0180000533.01.T01 |
| Chr1 | exon | 7490920 | 7491012 | 7490920 | ID=MsG0180000533.01.T01:exon:27605;Parent=MsG0180000533.01.T01 |
| Chr1 | CDS | 7492263 | 7492615 | 7492263 | ID=MsG0180000533.01.T01:cds;Parent=MsG0180000533.01.T01 |
| Chr1 | CDS | 7491976 | 7492174 | 7491976 | ID=MsG0180000533.01.T01:cds;Parent=MsG0180000533.01.T01 |
| Chr1 | CDS | 7491749 | 7491891 | 7491749 | ID=MsG0180000533.01.T01:cds;Parent=MsG0180000533.01.T01 |
| Chr1 | CDS | 7491108 | 7491660 | 7491108 | ID=MsG0180000533.01.T01:cds;Parent=MsG0180000533.01.T01 |
| Chr1 | CDS | 7490920 | 7491012 | 7490920 | ID=MsG0180000533.01.T01:cds;Parent=MsG0180000533.01.T01 |
| Gene Sequence |
| Protein sequence |
