Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038803.01.T01 | KEH22914.1 | 79.022 | 491 | 77 | 3 | 5 | 470 | 3 | 492 | 0 | 766 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038803.01.T01 | A0A072TZK3 | 79.022 | 491 | 77 | 3 | 5 | 470 | 3 | 492 | 0.0 | 766 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049058.01 | MsG0780038803.01 | 0.811167 | 8.014720e-51 | 4.925717e-48 |
| MsG0180000983.01 | MsG0780038803.01 | 0.806051 | 9.923234e-50 | 5.333267e-47 |
| MsG0180003656.01 | MsG0780038803.01 | 0.814477 | 1.510359e-51 | 1.014551e-48 |
| MsG0180004099.01 | MsG0780038803.01 | 0.824621 | 7.309186e-54 | 6.509386e-51 |
| MsG0180004970.01 | MsG0780038803.01 | 0.808731 | 2.681851e-50 | 1.545102e-47 |
| MsG0280006311.01 | MsG0780038803.01 | 0.820791 | 5.692126e-53 | 4.548099e-50 |
| MsG0280010779.01 | MsG0780038803.01 | 0.804132 | 2.501030e-49 | 1.279451e-46 |
| MsG0280011282.01 | MsG0780038803.01 | 0.831806 | 1.354963e-55 | 1.486657e-52 |
| MsG0380013636.01 | MsG0780038803.01 | 0.825992 | 3.462867e-54 | 3.206737e-51 |
| MsG0480018861.01 | MsG0780038803.01 | 0.806445 | 8.198923e-50 | 4.450964e-47 |
| MsG0480022331.01 | MsG0780038803.01 | 0.800508 | 1.394437e-48 | 6.504325e-46 |
| MsG0480023130.01 | MsG0780038803.01 | 0.850460 | 1.674456e-60 | 3.282254e-57 |
| MsG0480023832.01 | MsG0780038803.01 | 0.804530 | 2.067066e-49 | 1.068254e-46 |
| MsG0580024758.01 | MsG0780038803.01 | 0.837639 | 4.617274e-57 | 6.035583e-54 |
| MsG0380015190.01 | MsG0780038803.01 | 0.800544 | 1.371089e-48 | 6.401373e-46 |
| MsG0380015677.01 | MsG0780038803.01 | 0.806967 | 6.360539e-50 | 3.499548e-47 |
| MsG0380015805.01 | MsG0780038803.01 | 0.803816 | 2.910107e-49 | 1.476710e-46 |
| MsG0380016553.01 | MsG0780038803.01 | 0.815163 | 1.064332e-51 | 7.282445e-49 |
| MsG0380016603.01 | MsG0780038803.01 | 0.807942 | 3.950960e-50 | 2.229636e-47 |
| MsG0380016703.01 | MsG0780038803.01 | 0.828742 | 7.593075e-55 | 7.613385e-52 |
| MsG0580026307.01 | MsG0780038803.01 | 0.805535 | 1.274019e-49 | 6.756817e-47 |
| MsG0580029298.01 | MsG0780038803.01 | 0.806438 | 8.223873e-50 | 4.463735e-47 |
| MsG0280006591.01 | MsG0780038803.01 | 0.821127 | 4.763014e-53 | 3.841701e-50 |
| MsG0280008107.01 | MsG0780038803.01 | 0.813550 | 2.418477e-51 | 1.584227e-48 |
| MsG0280008280.01 | MsG0780038803.01 | 0.805677 | 1.189505e-49 | 6.331950e-47 |
| MsG0280008285.01 | MsG0780038803.01 | 0.807184 | 5.720829e-50 | 3.165479e-47 |
| MsG0280009835.01 | MsG0780038803.01 | 0.803181 | 3.940670e-49 | 1.967240e-46 |
| MsG0780036816.01 | MsG0780038803.01 | 0.805656 | 1.201624e-49 | 6.392969e-47 |
| MsG0780038803.01 | MsG0780038997.01 | 0.830537 | 2.778146e-55 | 2.935295e-52 |
| MsG0780038803.01 | MsG0780039059.01 | 0.809125 | 2.207937e-50 | 1.285606e-47 |
| MsG0780038803.01 | MsG0780039866.01 | 0.816178 | 6.320766e-52 | 4.447148e-49 |
| MsG0780038803.01 | MsG0780040669.01 | 0.814350 | 1.611409e-51 | 1.078659e-48 |
| MsG0780038803.01 | MsG0780040896.01 | 0.802690 | 4.978152e-49 | 2.454232e-46 |
| MsG0780038803.01 | MsG0780041667.01 | 0.809054 | 2.287382e-50 | 1.329255e-47 |
| MsG0780038803.01 | MsG0880046020.01 | 0.800609 | 1.330024e-48 | 6.219846e-46 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038803.01.T01 | MTR_7g062280 | 79.022 | 491 | 77 | 3 | 5 | 470 | 3 | 492 | 0.0 | 766 |
| MsG0780038803.01.T01 | MTR_7g062300 | 80.713 | 477 | 72 | 5 | 1 | 462 | 1 | 472 | 0.0 | 752 |
| MsG0780038803.01.T01 | MTR_7g063920 | 65.975 | 482 | 107 | 6 | 1 | 470 | 1 | 437 | 0.0 | 585 |
| MsG0780038803.01.T01 | MTR_7g063880 | 55.350 | 486 | 182 | 9 | 1 | 469 | 1 | 468 | 1.04e-162 | 468 |
| MsG0780038803.01.T01 | MTR_7g063660 | 53.942 | 482 | 197 | 8 | 7 | 470 | 4 | 478 | 3.02e-160 | 462 |
| MsG0780038803.01.T01 | MTR_7g063900 | 53.722 | 497 | 195 | 11 | 1 | 470 | 1 | 489 | 3.50e-157 | 455 |
| MsG0780038803.01.T01 | MTR_7g063840 | 54.298 | 477 | 197 | 8 | 8 | 470 | 5 | 474 | 6.82e-156 | 451 |
| MsG0780038803.01.T01 | MTR_7g063830 | 52.795 | 483 | 175 | 9 | 1 | 469 | 1 | 444 | 2.11e-150 | 436 |
| MsG0780038803.01.T01 | MTR_7g063820 | 52.333 | 493 | 204 | 10 | 1 | 470 | 1 | 485 | 3.79e-146 | 427 |
| MsG0780038803.01.T01 | MTR_3g007740 | 52.510 | 478 | 167 | 11 | 8 | 464 | 5 | 443 | 1.23e-141 | 414 |
| MsG0780038803.01.T01 | MTR_7g063890 | 52.664 | 488 | 195 | 12 | 10 | 469 | 25 | 504 | 2.75e-135 | 399 |
| MsG0780038803.01.T01 | MTR_7g063670 | 48.660 | 485 | 186 | 9 | 10 | 470 | 13 | 458 | 1.60e-127 | 378 |
| MsG0780038803.01.T01 | MTR_7g066570 | 40.244 | 492 | 253 | 9 | 4 | 467 | 6 | 484 | 3.02e-109 | 335 |
| MsG0780038803.01.T01 | MTR_7g066260 | 41.870 | 492 | 227 | 14 | 1 | 463 | 1 | 462 | 4.67e-101 | 311 |
| MsG0780038803.01.T01 | MTR_3g065200 | 44.144 | 444 | 205 | 13 | 12 | 450 | 3 | 408 | 4.48e-99 | 305 |
| MsG0780038803.01.T01 | MTR_7g066430 | 40.333 | 481 | 257 | 8 | 13 | 463 | 5 | 485 | 1.31e-97 | 303 |
| MsG0780038803.01.T01 | MTR_7g066410 | 38.477 | 512 | 254 | 11 | 10 | 465 | 8 | 514 | 2.77e-97 | 302 |
| MsG0780038803.01.T01 | MTR_7g058710 | 38.218 | 505 | 256 | 14 | 1 | 470 | 1 | 484 | 3.49e-95 | 296 |
| MsG0780038803.01.T01 | MTR_7g012680 | 39.024 | 492 | 238 | 14 | 1 | 470 | 1 | 452 | 6.75e-93 | 289 |
| MsG0780038803.01.T01 | MTR_7g066480 | 38.494 | 478 | 198 | 11 | 12 | 464 | 16 | 422 | 8.05e-90 | 280 |
| MsG0780038803.01.T01 | MTR_7g066580 | 38.866 | 476 | 224 | 10 | 24 | 466 | 7 | 448 | 8.32e-89 | 278 |
| MsG0780038803.01.T01 | MTR_7g066340 | 36.270 | 488 | 251 | 10 | 1 | 463 | 1 | 453 | 5.97e-87 | 275 |
| MsG0780038803.01.T01 | MTR_7g066350 | 37.657 | 478 | 241 | 12 | 11 | 465 | 8 | 451 | 8.28e-85 | 268 |
| MsG0780038803.01.T01 | MTR_7g066360 | 40.741 | 486 | 216 | 15 | 2 | 459 | 4 | 445 | 6.56e-84 | 266 |
| MsG0780038803.01.T01 | MTR_7g062270 | 69.399 | 183 | 55 | 1 | 6 | 187 | 3 | 185 | 9.08e-84 | 263 |
| MsG0780038803.01.T01 | MTR_7g066490 | 37.879 | 462 | 207 | 10 | 12 | 463 | 16 | 407 | 1.54e-83 | 264 |
| MsG0780038803.01.T01 | MTR_7g066290 | 38.540 | 493 | 213 | 13 | 1 | 463 | 1 | 433 | 1.01e-82 | 262 |
| MsG0780038803.01.T01 | MTR_7g066400 | 35.700 | 493 | 235 | 13 | 1 | 465 | 1 | 439 | 6.44e-81 | 258 |
| MsG0780038803.01.T01 | MTR_1g041315 | 34.167 | 480 | 248 | 9 | 3 | 447 | 5 | 451 | 3.86e-79 | 254 |
| MsG0780038803.01.T01 | MTR_1g014060 | 37.225 | 454 | 210 | 13 | 21 | 470 | 9 | 391 | 4.85e-79 | 251 |
| MsG0780038803.01.T01 | MTR_7g063720 | 53.785 | 251 | 94 | 5 | 223 | 470 | 2 | 233 | 5.40e-79 | 246 |
| MsG0780038803.01.T01 | MTR_7g063720 | 29.630 | 216 | 120 | 9 | 14 | 217 | 25 | 220 | 1.47e-12 | 67.8 |
| MsG0780038803.01.T01 | MTR_7g058700 | 35.135 | 481 | 195 | 11 | 24 | 470 | 531 | 928 | 9.76e-73 | 247 |
| MsG0780038803.01.T01 | MTR_7g066310 | 33.187 | 455 | 225 | 7 | 11 | 463 | 6 | 383 | 6.75e-69 | 225 |
| MsG0780038803.01.T01 | MTR_4g019310 | 33.865 | 502 | 289 | 15 | 6 | 470 | 2 | 497 | 2.01e-68 | 227 |
| MsG0780038803.01.T01 | MTR_7g063850 | 44.590 | 305 | 135 | 9 | 182 | 469 | 1 | 288 | 4.17e-62 | 204 |
| MsG0780038803.01.T01 | MTR_2g029400 | 31.799 | 478 | 224 | 10 | 2 | 453 | 4 | 405 | 2.18e-60 | 203 |
| MsG0780038803.01.T01 | MTR_4g017830 | 30.651 | 522 | 294 | 16 | 1 | 469 | 1 | 507 | 5.22e-53 | 187 |
| MsG0780038803.01.T01 | MTR_7g066280 | 32.071 | 396 | 240 | 10 | 91 | 463 | 1 | 390 | 3.98e-50 | 176 |
| MsG0780038803.01.T01 | MTR_4g017440 | 30.077 | 522 | 298 | 17 | 1 | 469 | 1 | 508 | 3.90e-47 | 170 |
| MsG0780038803.01.T01 | MTR_7g100420 | 38.629 | 321 | 161 | 8 | 156 | 459 | 92 | 393 | 4.38e-47 | 168 |
| MsG0780038803.01.T01 | MTR_7g100420 | 49.398 | 83 | 39 | 2 | 6 | 86 | 33 | 114 | 4.40e-12 | 68.2 |
| MsG0780038803.01.T01 | MTR_4g017610 | 30.261 | 499 | 279 | 17 | 5 | 459 | 2 | 475 | 2.48e-46 | 167 |
| MsG0780038803.01.T01 | MTR_4g017410 | 30.593 | 523 | 269 | 19 | 6 | 469 | 3 | 490 | 2.18e-45 | 166 |
| MsG0780038803.01.T01 | MTR_4g019340 | 30.567 | 494 | 263 | 18 | 10 | 451 | 36 | 501 | 2.05e-44 | 163 |
| MsG0780038803.01.T01 | MTR_7g066680 | 34.304 | 309 | 141 | 5 | 201 | 459 | 38 | 334 | 1.91e-42 | 154 |
| MsG0780038803.01.T01 | MTR_7g066680 | 27.222 | 180 | 119 | 6 | 6 | 179 | 80 | 253 | 3.24e-12 | 68.2 |
| MsG0780038803.01.T01 | MTR_4g017470 | 28.046 | 517 | 283 | 15 | 1 | 469 | 1 | 476 | 3.58e-41 | 154 |
| MsG0780038803.01.T01 | MTR_4g017550 | 29.350 | 477 | 250 | 19 | 42 | 459 | 21 | 469 | 5.71e-32 | 127 |
| MsG0780038803.01.T01 | MTR_4g017550 | 30.645 | 186 | 97 | 7 | 13 | 179 | 248 | 420 | 6.23e-12 | 67.8 |
| MsG0780038803.01.T01 | MTR_8g023240 | 25.732 | 478 | 290 | 16 | 13 | 457 | 38 | 483 | 6.09e-31 | 125 |
| MsG0780038803.01.T01 | MTR_7g063690 | 42.759 | 145 | 69 | 2 | 140 | 270 | 78 | 222 | 5.51e-27 | 109 |
| MsG0780038803.01.T01 | MTR_7g063690 | 70.909 | 55 | 15 | 1 | 8 | 61 | 7 | 61 | 1.11e-15 | 77.0 |
| MsG0780038803.01.T01 | MTR_7g063760 | 56.757 | 74 | 31 | 1 | 398 | 470 | 1 | 74 | 8.27e-22 | 89.4 |
| MsG0780038803.01.T01 | MTR_8g445860 | 31.967 | 244 | 130 | 9 | 3 | 223 | 8 | 238 | 1.51e-21 | 94.0 |
| MsG0780038803.01.T01 | MTR_1g115440 | 31.169 | 231 | 135 | 9 | 243 | 457 | 11 | 233 | 4.07e-21 | 92.4 |
| MsG0780038803.01.T01 | MTR_1g115440 | 31.746 | 252 | 128 | 11 | 1 | 222 | 1 | 238 | 1.38e-19 | 88.2 |
| MsG0780038803.01.T01 | MTR_7g066390 | 54.795 | 73 | 33 | 0 | 391 | 463 | 50 | 122 | 7.41e-19 | 84.0 |
| MsG0780038803.01.T01 | MTR_0150s0010 | 29.268 | 246 | 135 | 9 | 5 | 222 | 3 | 237 | 3.40e-18 | 84.3 |
| MsG0780038803.01.T01 | MTR_2g067310 | 35.393 | 178 | 75 | 6 | 18 | 195 | 53 | 190 | 1.76e-15 | 76.3 |
| MsG0780038803.01.T01 | MTR_7g063870 | 55.357 | 56 | 25 | 0 | 6 | 61 | 25 | 80 | 4.63e-13 | 69.3 |
| MsG0780038803.01.T01 | MTR_1g115455 | 40.000 | 95 | 49 | 2 | 1 | 95 | 1 | 87 | 3.91e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780038803.01.T01 | AT3G09140 | 32.108 | 517 | 277 | 16 | 1 | 465 | 1 | 495 | 1.82e-61 | 209 |
| MsG0780038803.01.T01 | AT3G09140 | 30.459 | 545 | 277 | 17 | 1 | 465 | 1 | 523 | 5.25e-57 | 197 |
| MsG0780038803.01.T01 | AT5G01120 | 30.815 | 503 | 271 | 14 | 13 | 462 | 10 | 488 | 1.46e-56 | 196 |
| MsG0780038803.01.T01 | AT5G01150 | 30.402 | 523 | 264 | 16 | 7 | 469 | 4 | 486 | 1.72e-56 | 195 |
| MsG0780038803.01.T01 | AT5G01130 | 30.879 | 489 | 272 | 12 | 7 | 470 | 4 | 451 | 2.55e-55 | 191 |
| MsG0780038803.01.T01 | AT5G43240 | 31.663 | 499 | 270 | 18 | 13 | 459 | 10 | 489 | 1.11e-49 | 177 |
| MsG0780038803.01.T01 | AT5G43240 | 31.663 | 499 | 270 | 18 | 13 | 459 | 10 | 489 | 1.11e-49 | 177 |
| MsG0780038803.01.T01 | AT5G43240 | 31.663 | 499 | 270 | 18 | 13 | 459 | 10 | 489 | 1.11e-49 | 177 |
| MsG0780038803.01.T01 | AT3G09110 | 34.639 | 332 | 190 | 7 | 14 | 326 | 11 | 334 | 1.13e-49 | 173 |
| MsG0780038803.01.T01 | AT5G01140 | 28.340 | 494 | 285 | 14 | 7 | 470 | 4 | 458 | 1.08e-47 | 171 |
| MsG0780038803.01.T01 | AT3G09120 | 35.714 | 322 | 159 | 9 | 11 | 297 | 5 | 313 | 3.85e-46 | 163 |
| MsG0780038803.01.T01 | AT3G09120 | 27.500 | 240 | 133 | 6 | 243 | 457 | 6 | 229 | 8.21e-11 | 63.5 |
| MsG0780038803.01.T01 | AT5G37320 | 28.571 | 504 | 277 | 18 | 13 | 465 | 10 | 481 | 2.19e-40 | 151 |
| MsG0780038803.01.T01 | AT5G43240 | 28.736 | 348 | 190 | 14 | 155 | 459 | 13 | 345 | 2.84e-15 | 77.8 |
Find 85 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGGAATTGTTCGAATATT+TGG | 0.115220 | 7:+53262505 | MsG0780038803.01.T01:CDS |
| TAACTAGCTTCAGAATAATT+TGG | 0.160980 | 7:-53262396 | None:intergenic |
| TTTAGTCTGTTCAAGGAATT+TGG | 0.172599 | 7:+53262108 | MsG0780038803.01.T01:CDS |
| ATCCTTGGCGGAGAGATTTA+AGG | 0.228622 | 7:-53262011 | None:intergenic |
| CTCCGCCAAGGATTTGTTAA+TGG | 0.248773 | 7:+53262021 | MsG0780038803.01.T01:CDS |
| CTTGACTCCAATTTCAGCTT+TGG | 0.260143 | 7:+53262897 | MsG0780038803.01.T01:CDS |
| CCTGGTGTTCATGAATATTA+TGG | 0.302474 | 7:+53262688 | MsG0780038803.01.T01:CDS |
| GTATTTCGCTACAGATGATT+TGG | 0.304476 | 7:+53262864 | MsG0780038803.01.T01:CDS |
| TGATTGCTTAACTTTAACTC+TGG | 0.316147 | 7:-53262646 | None:intergenic |
| CTATAAAAGTGTGGCAGATC+TGG | 0.332749 | 7:+53261762 | MsG0780038803.01.T01:CDS |
| AGTAAACCCAAAGCTGAAAT+TGG | 0.334732 | 7:-53262904 | None:intergenic |
| TTGACTCCAATTTCAGCTTT+GGG | 0.338051 | 7:+53262898 | MsG0780038803.01.T01:CDS |
| CAGTAATAATGATATTGTTT+TGG | 0.346570 | 7:+53262732 | MsG0780038803.01.T01:CDS |
| CTTGTATAATCAATACAGTT+TGG | 0.351386 | 7:-53262084 | None:intergenic |
| AGATTCTTAACAATGCCTTT+AGG | 0.354347 | 7:+53261670 | MsG0780038803.01.T01:CDS |
| TTCGTTCAAAAGAAGTTTCA+AGG | 0.367404 | 7:-53261590 | None:intergenic |
| TGAACACCAGGTTCAAGAAT+TGG | 0.375903 | 7:-53262676 | None:intergenic |
| TTCTGTGATGTTGAAATTAG+TGG | 0.377162 | 7:+53262367 | MsG0780038803.01.T01:CDS |
| TACAAGCTTTAGTCTGTTCA+AGG | 0.384290 | 7:+53262101 | MsG0780038803.01.T01:CDS |
| TACTCCTCTTGCTGATTTGA+AGG | 0.385097 | 7:+53262939 | MsG0780038803.01.T01:CDS |
| ATCTGCCTTAACAAATGGTT+TGG | 0.386717 | 7:+53263160 | MsG0780038803.01.T01:CDS |
| TTTAGGAACCATTGCGAGAT+TGG | 0.390984 | 7:+53261687 | MsG0780038803.01.T01:CDS |
| AAAGTCTTGTTTGCAGAAGC+AGG | 0.413091 | 7:+53261622 | MsG0780038803.01.T01:CDS |
| TCAACATCTGCCTTAACAAA+TGG | 0.422887 | 7:+53263155 | MsG0780038803.01.T01:CDS |
| AACCATCCCTTGAGAAATCA+AGG | 0.425614 | 7:+53262338 | MsG0780038803.01.T01:CDS |
| GCTTTCCAAGACAACTTTGA+CGG | 0.432123 | 7:+53262297 | MsG0780038803.01.T01:CDS |
| TTTGGAGAGAATTGTTCCTT+TGG | 0.438718 | 7:+53262523 | MsG0780038803.01.T01:CDS |
| GATTGCTTAACTTTAACTCT+GGG | 0.444533 | 7:-53262645 | None:intergenic |
| CAAATCCGTCAAAGTTGTCT+TGG | 0.450921 | 7:-53262302 | None:intergenic |
| GTGAGCCAAACCATTTGTTA+AGG | 0.458136 | 7:-53263165 | None:intergenic |
| TCACATGTTAACCGAAGTTA+AGG | 0.465854 | 7:+53263184 | MsG0780038803.01.T01:CDS |
| TAAATATTTGATATCAAAAG+AGG | 0.486512 | 7:+53262591 | MsG0780038803.01.T01:CDS |
| GGTGTAGATAAGAGTTATGC+TGG | 0.489254 | 7:+53262772 | MsG0780038803.01.T01:CDS |
| TACTGGGGTGATTCCTTGTG+AGG | 0.493234 | 7:+53262819 | MsG0780038803.01.T01:CDS |
| TTTCTTACATTTCCTTTGGG+TGG | 0.496782 | 7:+53262487 | MsG0780038803.01.T01:CDS |
| AACCTTGATTTCTCAAGGGA+TGG | 0.498568 | 7:-53262340 | None:intergenic |
| AGATAAACCTTGATTTCTCA+AGG | 0.509788 | 7:-53262345 | None:intergenic |
| TCTGTAGCGAAATACATAGT+TGG | 0.510023 | 7:-53262856 | None:intergenic |
| CAACATTTGTTTGATTGTCT+CGG | 0.512040 | 7:-53261803 | None:intergenic |
| AGTTGTCACTATTGGCATCA+AGG | 0.520473 | 7:+53262966 | MsG0780038803.01.T01:CDS |
| AACAAGGTATTGTATGCTCA+AGG | 0.521643 | 7:+53262433 | MsG0780038803.01.T01:CDS |
| CAGCAAGAGGAGTATTCAAT+CGG | 0.523415 | 7:-53262930 | None:intergenic |
| TCATTTCTCCTCCTTAACTT+CGG | 0.524075 | 7:-53263195 | None:intergenic |
| CCTTGTGAGGGTTATGTGAA+GGG | 0.527421 | 7:+53262832 | MsG0780038803.01.T01:CDS |
| TATCAAAAGAGGCTAAGAAC+AGG | 0.528682 | 7:+53262602 | MsG0780038803.01.T01:CDS |
| GCGATAGAAGCAAACACTAA+AGG | 0.532003 | 7:-53261917 | None:intergenic |
| CAAGGTATTGTATGCTCAAG+GGG | 0.532821 | 7:+53262435 | MsG0780038803.01.T01:CDS |
| CTACACCATTAACAAATCCT+TGG | 0.535180 | 7:-53262026 | None:intergenic |
| GCACATGTGAAGTATATTGT+TGG | 0.537576 | 7:-53261892 | None:intergenic |
| CTTGTTTGCAGAAGCAGGGA+AGG | 0.539743 | 7:+53261627 | MsG0780038803.01.T01:CDS |
| TTCCTTAAATCTCTCCGCCA+AGG | 0.540627 | 7:+53262009 | MsG0780038803.01.T01:CDS |
| CAAAGCTGAAATTGGAGTCA+AGG | 0.541710 | 7:-53262896 | None:intergenic |
| ATATTACCAATTCTTGAACC+TGG | 0.551456 | 7:+53262670 | MsG0780038803.01.T01:CDS |
| CACCATTAACAAATCCTTGG+CGG | 0.552287 | 7:-53262023 | None:intergenic |
| TTTAACTCTGGGGCAATGCA+AGG | 0.554669 | 7:-53262634 | None:intergenic |
| AGAAGCAGGGAAGGACTTTG+TGG | 0.556414 | 7:+53261636 | MsG0780038803.01.T01:CDS |
| CCATAATATTCATGAACACC+AGG | 0.559401 | 7:-53262688 | None:intergenic |
| AAAGCTGAAATTGGAGTCAA+GGG | 0.571174 | 7:-53262895 | None:intergenic |
| AAGTCTTGTTTGCAGAAGCA+GGG | 0.574475 | 7:+53261623 | MsG0780038803.01.T01:CDS |
| TCCTTGTGAGGGTTATGTGA+AGG | 0.575859 | 7:+53262831 | MsG0780038803.01.T01:CDS |
| CATTGCGAGATTGGTAGAGA+AGG | 0.577515 | 7:+53261696 | MsG0780038803.01.T01:CDS |
| TGTTCCTTTGGAAGCATCAG+CGG | 0.578272 | 7:+53262535 | MsG0780038803.01.T01:CDS |
| TAAAGTTCCGCTGAGTTCGT+TGG | 0.578493 | 7:-53261832 | None:intergenic |
| AATCTCGCAATGGTTCCTAA+AGG | 0.583079 | 7:-53261685 | None:intergenic |
| TAGAGAAGGAATCTAGCATA+GGG | 0.584603 | 7:+53261710 | MsG0780038803.01.T01:CDS |
| AGCATAGGGCAAGTTACAGT+CGG | 0.590026 | 7:+53261724 | MsG0780038803.01.T01:CDS |
| ACTGGGGTGATTCCTTGTGA+GGG | 0.590492 | 7:+53262820 | MsG0780038803.01.T01:CDS |
| ACAAGGTATTGTATGCTCAA+GGG | 0.592568 | 7:+53262434 | MsG0780038803.01.T01:CDS |
| GTAGAGAAGGAATCTAGCAT+AGG | 0.592587 | 7:+53261709 | MsG0780038803.01.T01:CDS |
| CTTCTCTACCAATCTCGCAA+TGG | 0.597488 | 7:-53261695 | None:intergenic |
| GATAAACCTTGATTTCTCAA+GGG | 0.605433 | 7:-53262344 | None:intergenic |
| GCATAACTCTTATCTACACC+AGG | 0.610698 | 7:-53262769 | None:intergenic |
| CATGTTAACCGAAGTTAAGG+AGG | 0.619389 | 7:+53263187 | MsG0780038803.01.T01:CDS |
| AAACTCACTCTATAAAAGTG+TGG | 0.625241 | 7:+53261753 | MsG0780038803.01.T01:CDS |
| CCCTTCACATAACCCTCACA+AGG | 0.628562 | 7:-53262832 | None:intergenic |
| TATAAGAAAATCAGATAACA+AGG | 0.631180 | 7:+53262417 | MsG0780038803.01.T01:CDS |
| GATAAACATAAATGTCGATG+TGG | 0.637729 | 7:+53261964 | MsG0780038803.01.T01:CDS |
| ATTCGAACAATTCCACCCAA+AGG | 0.640069 | 7:-53262499 | None:intergenic |
| TACTACTTCTACTCAAGCAG+AGG | 0.643833 | 7:+53261558 | MsG0780038803.01.T01:CDS |
| AGTGTGGCAGATCTGGATGT+AGG | 0.651804 | 7:+53261769 | MsG0780038803.01.T01:CDS |
| CAATCCGCTGATGCTTCCAA+AGG | 0.653547 | 7:-53262539 | None:intergenic |
| ATAGAAGCAAACACTAAAGG+CGG | 0.658526 | 7:-53261914 | None:intergenic |
| ATTGCTTAACTTTAACTCTG+GGG | 0.673440 | 7:-53262644 | None:intergenic |
| GCTGCAACCAACGAACTCAG+CGG | 0.734689 | 7:+53261825 | MsG0780038803.01.T01:CDS |
| TGTCACTATTGGCATCAAGG+AGG | 0.742676 | 7:+53262969 | MsG0780038803.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTACTATTTTATATATAC+AGG | + | Chr7:53262249-53262268 | MsG0780038803.01.T01:intron | 10.0% |
| !! | ATAAAAATGAATTTATCTAC+TGG | + | Chr7:53262802-53262821 | MsG0780038803.01.T01:CDS | 15.0% |
| !! | TAAAAATGAATTTATCTACT+GGG | + | Chr7:53262803-53262822 | MsG0780038803.01.T01:CDS | 15.0% |
| !! | TAAATATTTGATATCAAAAG+AGG | + | Chr7:53262591-53262610 | MsG0780038803.01.T01:CDS | 15.0% |
| !!! | GTCTAATATAAATTTTACTA+TGG | - | Chr7:53262999-53263018 | None:intergenic | 15.0% |
| !! | AAAAATGAATTTATCTACTG+GGG | + | Chr7:53262804-53262823 | MsG0780038803.01.T01:CDS | 20.0% |
| !! | TATAAGAAAATCAGATAACA+AGG | + | Chr7:53262417-53262436 | MsG0780038803.01.T01:CDS | 20.0% |
| !! | TATTTAAATGTTCTATGCTT+GGG | + | Chr7:53263033-53263052 | MsG0780038803.01.T01:intron | 20.0% |
| !! | TTATTTAAATGTTCTATGCT+TGG | + | Chr7:53263032-53263051 | MsG0780038803.01.T01:intron | 20.0% |
| !!! | CAGTAATAATGATATTGTTT+TGG | + | Chr7:53262732-53262751 | MsG0780038803.01.T01:CDS | 20.0% |
| !!! | TATGGTTATTTTAAAAGTGT+TGG | + | Chr7:53262706-53262725 | MsG0780038803.01.T01:CDS | 20.0% |
| ! | ATTTAAATGTTCTATGCTTG+GGG | + | Chr7:53263034-53263053 | MsG0780038803.01.T01:intron | 25.0% |
| ! | CTTGTATAATCAATACAGTT+TGG | - | Chr7:53262087-53262106 | None:intergenic | 25.0% |
| !! | AAACTTCTTTTGAACGAAAA+TGG | + | Chr7:53261595-53261614 | MsG0780038803.01.T01:CDS | 25.0% |
| !! | AAGTTTTCTTACATTTCCTT+TGG | + | Chr7:53262483-53262502 | MsG0780038803.01.T01:CDS | 25.0% |
| !! | AGTTTTCTTACATTTCCTTT+GGG | + | Chr7:53262484-53262503 | MsG0780038803.01.T01:CDS | 25.0% |
| !! | TAACTAGCTTCAGAATAATT+TGG | - | Chr7:53262399-53262418 | None:intergenic | 25.0% |
| AAACTCACTCTATAAAAGTG+TGG | + | Chr7:53261753-53261772 | MsG0780038803.01.T01:CDS | 30.0% | |
| AACACTACAACAAGACAATA+AGG | - | Chr7:53263109-53263128 | None:intergenic | 30.0% | |
| AAGGAAAAAGTTGTCACTAT+TGG | + | Chr7:53262958-53262977 | MsG0780038803.01.T01:CDS | 30.0% | |
| AGATAAACCTTGATTTCTCA+AGG | - | Chr7:53262348-53262367 | None:intergenic | 30.0% | |
| AGATTCTTAACAATGCCTTT+AGG | + | Chr7:53261670-53261689 | MsG0780038803.01.T01:CDS | 30.0% | |
| ATATTACCAATTCTTGAACC+TGG | + | Chr7:53262670-53262689 | MsG0780038803.01.T01:CDS | 30.0% | |
| ATTGCTTAACTTTAACTCTG+GGG | - | Chr7:53262647-53262666 | None:intergenic | 30.0% | |
| CAACATTTGTTTGATTGTCT+CGG | - | Chr7:53261806-53261825 | None:intergenic | 30.0% | |
| GATAAACATAAATGTCGATG+TGG | + | Chr7:53261964-53261983 | MsG0780038803.01.T01:CDS | 30.0% | |
| GATAAACCTTGATTTCTCAA+GGG | - | Chr7:53262347-53262366 | None:intergenic | 30.0% | |
| GATTGCTTAACTTTAACTCT+GGG | - | Chr7:53262648-53262667 | None:intergenic | 30.0% | |
| TGATTGCTTAACTTTAACTC+TGG | - | Chr7:53262649-53262668 | None:intergenic | 30.0% | |
| TTCGTTCAAAAGAAGTTTCA+AGG | - | Chr7:53261593-53261612 | None:intergenic | 30.0% | |
| TTCTGTGATGTTGAAATTAG+TGG | + | Chr7:53262367-53262386 | MsG0780038803.01.T01:CDS | 30.0% | |
| TTTAAATGTTCTATGCTTGG+GGG | + | Chr7:53263035-53263054 | MsG0780038803.01.T01:intron | 30.0% | |
| TTTAGTCTGTTCAAGGAATT+TGG | + | Chr7:53262108-53262127 | MsG0780038803.01.T01:CDS | 30.0% | |
| ! | TTGGTTCAAATTTTCAAACC+TGG | + | Chr7:53262751-53262770 | MsG0780038803.01.T01:CDS | 30.0% |
| !!! | GTAGTGTTTTAGCATTTTGA+AGG | + | Chr7:53263121-53263140 | MsG0780038803.01.T01:intron | 30.0% |
| AAAGCTGAAATTGGAGTCAA+GGG | - | Chr7:53262898-53262917 | None:intergenic | 35.0% | |
| AGTAAACCCAAAGCTGAAAT+TGG | - | Chr7:53262907-53262926 | None:intergenic | 35.0% | |
| ATAGAAGCAAACACTAAAGG+CGG | - | Chr7:53261917-53261936 | None:intergenic | 35.0% | |
| ATCTGCCTTAACAAATGGTT+TGG | + | Chr7:53263160-53263179 | MsG0780038803.01.T01:CDS | 35.0% | |
| CCATAATATTCATGAACACC+AGG | - | Chr7:53262691-53262710 | None:intergenic | 35.0% | |
| CCTGGTGTTCATGAATATTA+TGG | + | Chr7:53262688-53262707 | MsG0780038803.01.T01:CDS | 35.0% | |
| CTACACCATTAACAAATCCT+TGG | - | Chr7:53262029-53262048 | None:intergenic | 35.0% | |
| GCACATGTGAAGTATATTGT+TGG | - | Chr7:53261895-53261914 | None:intergenic | 35.0% | |
| GGTGGAATTGTTCGAATATT+TGG | + | Chr7:53262505-53262524 | MsG0780038803.01.T01:CDS | 35.0% | |
| GTATTTCGCTACAGATGATT+TGG | + | Chr7:53262864-53262883 | MsG0780038803.01.T01:CDS | 35.0% | |
| TAGAGAAGGAATCTAGCATA+GGG | + | Chr7:53261710-53261729 | MsG0780038803.01.T01:CDS | 35.0% | |
| TATCAAAAGAGGCTAAGAAC+AGG | + | Chr7:53262602-53262621 | MsG0780038803.01.T01:CDS | 35.0% | |
| TCAACATCTGCCTTAACAAA+TGG | + | Chr7:53263155-53263174 | MsG0780038803.01.T01:CDS | 35.0% | |
| TCACATGTTAACCGAAGTTA+AGG | + | Chr7:53263184-53263203 | MsG0780038803.01.T01:CDS | 35.0% | |
| TCATTTCTCCTCCTTAACTT+CGG | - | Chr7:53263198-53263217 | None:intergenic | 35.0% | |
| TCTGAATGTCACCAAAGAAA+AGG | + | Chr7:53262164-53262183 | MsG0780038803.01.T01:intron | 35.0% | |
| TCTGTAGCGAAATACATAGT+TGG | - | Chr7:53262859-53262878 | None:intergenic | 35.0% | |
| TGGCATAAAAAACCCTAGTT+CGG | + | Chr7:53262128-53262147 | MsG0780038803.01.T01:CDS | 35.0% | |
| TTGACTCCAATTTCAGCTTT+GGG | + | Chr7:53262898-53262917 | MsG0780038803.01.T01:CDS | 35.0% | |
| TTTCTTACATTTCCTTTGGG+TGG | + | Chr7:53262487-53262506 | MsG0780038803.01.T01:CDS | 35.0% | |
| TTTGGAGAGAATTGTTCCTT+TGG | + | Chr7:53262523-53262542 | MsG0780038803.01.T01:CDS | 35.0% | |
| ! | CAAGCACAAAACCTTTTCTT+TGG | - | Chr7:53262178-53262197 | None:intergenic | 35.0% |
| ! | TACAAGCTTTAGTCTGTTCA+AGG | + | Chr7:53262101-53262120 | MsG0780038803.01.T01:CDS | 35.0% |
| ! | TTTTCCTTCAAATCAGCAAG+AGG | - | Chr7:53262946-53262965 | None:intergenic | 35.0% |
| !! | AACAAGGTATTGTATGCTCA+AGG | + | Chr7:53262433-53262452 | MsG0780038803.01.T01:CDS | 35.0% |
| !! | ACAAGGTATTGTATGCTCAA+GGG | + | Chr7:53262434-53262453 | MsG0780038803.01.T01:CDS | 35.0% |
| AACCATCCCTTGAGAAATCA+AGG | + | Chr7:53262338-53262357 | MsG0780038803.01.T01:CDS | 40.0% | |
| AACCTTGATTTCTCAAGGGA+TGG | - | Chr7:53262343-53262362 | None:intergenic | 40.0% | |
| AATCTCGCAATGGTTCCTAA+AGG | - | Chr7:53261688-53261707 | None:intergenic | 40.0% | |
| AGTTGTCACTATTGGCATCA+AGG | + | Chr7:53262966-53262985 | MsG0780038803.01.T01:CDS | 40.0% | |
| ATTCGAACAATTCCACCCAA+AGG | - | Chr7:53262502-53262521 | None:intergenic | 40.0% | |
| CAAAGCTGAAATTGGAGTCA+AGG | - | Chr7:53262899-53262918 | None:intergenic | 40.0% | |
| CAAATCCGTCAAAGTTGTCT+TGG | - | Chr7:53262305-53262324 | None:intergenic | 40.0% | |
| CACCATTAACAAATCCTTGG+CGG | - | Chr7:53262026-53262045 | None:intergenic | 40.0% | |
| CATGTTAACCGAAGTTAAGG+AGG | + | Chr7:53263187-53263206 | MsG0780038803.01.T01:CDS | 40.0% | |
| CTTGACTCCAATTTCAGCTT+TGG | + | Chr7:53262897-53262916 | MsG0780038803.01.T01:CDS | 40.0% | |
| GCATAACTCTTATCTACACC+AGG | - | Chr7:53262772-53262791 | None:intergenic | 40.0% | |
| GCGATAGAAGCAAACACTAA+AGG | - | Chr7:53261920-53261939 | None:intergenic | 40.0% | |
| GCTTTCCAAGACAACTTTGA+CGG | + | Chr7:53262297-53262316 | MsG0780038803.01.T01:CDS | 40.0% | |
| GGTGTAGATAAGAGTTATGC+TGG | + | Chr7:53262772-53262791 | MsG0780038803.01.T01:CDS | 40.0% | |
| GTAGAGAAGGAATCTAGCAT+AGG | + | Chr7:53261709-53261728 | MsG0780038803.01.T01:CDS | 40.0% | |
| TACTACTTCTACTCAAGCAG+AGG | + | Chr7:53261558-53261577 | MsG0780038803.01.T01:CDS | 40.0% | |
| TCAAGCTAACGCACCAAAAT+TGG | - | Chr7:53263064-53263083 | None:intergenic | 40.0% | |
| TGAACACCAGGTTCAAGAAT+TGG | - | Chr7:53262679-53262698 | None:intergenic | 40.0% | |
| TTTAGGAACCATTGCGAGAT+TGG | + | Chr7:53261687-53261706 | MsG0780038803.01.T01:CDS | 40.0% | |
| ! | ACCACTTCTTTTACCGAACT+AGG | - | Chr7:53262144-53262163 | None:intergenic | 40.0% |
| ! | CCACTTCTTTTACCGAACTA+GGG | - | Chr7:53262143-53262162 | None:intergenic | 40.0% |
| ! | CTATAAAAGTGTGGCAGATC+TGG | + | Chr7:53261762-53261781 | MsG0780038803.01.T01:CDS | 40.0% |
| ! | GTGAGCCAAACCATTTGTTA+AGG | - | Chr7:53263168-53263187 | None:intergenic | 40.0% |
| !! | AAAGTCTTGTTTGCAGAAGC+AGG | + | Chr7:53261622-53261641 | MsG0780038803.01.T01:CDS | 40.0% |
| !! | AAGTCTTGTTTGCAGAAGCA+GGG | + | Chr7:53261623-53261642 | MsG0780038803.01.T01:CDS | 40.0% |
| !! | CAAGGTATTGTATGCTCAAG+GGG | + | Chr7:53262435-53262454 | MsG0780038803.01.T01:CDS | 40.0% |
| !! | CAGCAAGAGGAGTATTCAAT+CGG | - | Chr7:53262933-53262952 | None:intergenic | 40.0% |
| !! | TACTCCTCTTGCTGATTTGA+AGG | + | Chr7:53262939-53262958 | MsG0780038803.01.T01:CDS | 40.0% |
| AGCATAGGGCAAGTTACAGT+CGG | + | Chr7:53261724-53261743 | MsG0780038803.01.T01:CDS | 45.0% | |
| ATCCTTGGCGGAGAGATTTA+AGG | - | Chr7:53262014-53262033 | None:intergenic | 45.0% | |
| CATTGCGAGATTGGTAGAGA+AGG | + | Chr7:53261696-53261715 | MsG0780038803.01.T01:CDS | 45.0% | |
| CCCTAGTTCGGTAAAAGAAG+TGG | + | Chr7:53262140-53262159 | MsG0780038803.01.T01:intron | 45.0% | |
| CCTTGTGAGGGTTATGTGAA+GGG | + | Chr7:53262832-53262851 | MsG0780038803.01.T01:CDS | 45.0% | |
| CTTCTCTACCAATCTCGCAA+TGG | - | Chr7:53261698-53261717 | None:intergenic | 45.0% | |
| TAAAGTTCCGCTGAGTTCGT+TGG | - | Chr7:53261835-53261854 | None:intergenic | 45.0% | |
| TCCTTGTGAGGGTTATGTGA+AGG | + | Chr7:53262831-53262850 | MsG0780038803.01.T01:CDS | 45.0% | |
| TGTCACTATTGGCATCAAGG+AGG | + | Chr7:53262969-53262988 | MsG0780038803.01.T01:CDS | 45.0% | |
| TTCCTTAAATCTCTCCGCCA+AGG | + | Chr7:53262009-53262028 | MsG0780038803.01.T01:CDS | 45.0% | |
| !! | CTCCGCCAAGGATTTGTTAA+TGG | + | Chr7:53262021-53262040 | MsG0780038803.01.T01:CDS | 45.0% |
| !! | TGTTCCTTTGGAAGCATCAG+CGG | + | Chr7:53262535-53262554 | MsG0780038803.01.T01:CDS | 45.0% |
| !! | TTTAACTCTGGGGCAATGCA+AGG | - | Chr7:53262637-53262656 | None:intergenic | 45.0% |
| ACTGGGGTGATTCCTTGTGA+GGG | + | Chr7:53262820-53262839 | MsG0780038803.01.T01:CDS | 50.0% | |
| AGAAGCAGGGAAGGACTTTG+TGG | + | Chr7:53261636-53261655 | MsG0780038803.01.T01:CDS | 50.0% | |
| AGTGTGGCAGATCTGGATGT+AGG | + | Chr7:53261769-53261788 | MsG0780038803.01.T01:CDS | 50.0% | |
| CAATCCGCTGATGCTTCCAA+AGG | - | Chr7:53262542-53262561 | None:intergenic | 50.0% | |
| CCCTTCACATAACCCTCACA+AGG | - | Chr7:53262835-53262854 | None:intergenic | 50.0% | |
| CTTGTTTGCAGAAGCAGGGA+AGG | + | Chr7:53261627-53261646 | MsG0780038803.01.T01:CDS | 50.0% | |
| TACTGGGGTGATTCCTTGTG+AGG | + | Chr7:53262819-53262838 | MsG0780038803.01.T01:CDS | 50.0% | |
| ! | TGCTTGGGGGTTGCCAATTT+TGG | + | Chr7:53263048-53263067 | MsG0780038803.01.T01:intron | 50.0% |
| GCTGCAACCAACGAACTCAG+CGG | + | Chr7:53261825-53261844 | MsG0780038803.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 53261550 | 53263217 | 53261550 | ID=MsG0780038803.01;Name=MsG0780038803.01 |
| Chr7 | mRNA | 53261550 | 53263217 | 53261550 | ID=MsG0780038803.01.T01;Parent=MsG0780038803.01;Name=MsG0780038803.01.T01;_AED=0.43;_eAED=0.43;_QI=0|0|0|1|1|1|3|0|470 |
| Chr7 | exon | 53261550 | 53262149 | 53261550 | ID=MsG0780038803.01.T01:exon:8476;Parent=MsG0780038803.01.T01 |
| Chr7 | exon | 53262271 | 53262990 | 53262271 | ID=MsG0780038803.01.T01:exon:8477;Parent=MsG0780038803.01.T01 |
| Chr7 | exon | 53263125 | 53263217 | 53263125 | ID=MsG0780038803.01.T01:exon:8478;Parent=MsG0780038803.01.T01 |
| Chr7 | CDS | 53261550 | 53262149 | 53261550 | ID=MsG0780038803.01.T01:cds;Parent=MsG0780038803.01.T01 |
| Chr7 | CDS | 53262271 | 53262990 | 53262271 | ID=MsG0780038803.01.T01:cds;Parent=MsG0780038803.01.T01 |
| Chr7 | CDS | 53263125 | 53263217 | 53263125 | ID=MsG0780038803.01.T01:cds;Parent=MsG0780038803.01.T01 |
| Gene Sequence |
| Protein sequence |
