AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001109.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001109.01.T01 MTR_1g030840 95.758 165 7 0 1 165 1 165 8.58e-116 325
MsG0180001109.01.T01 MTR_1g030810 59.355 155 58 2 1 155 1 150 4.25e-62 189
MsG0180001109.01.T01 MTR_1g030820 57.595 158 62 2 1 158 1 153 5.07e-62 188
MsG0180001109.01.T01 MTR_8g045555 38.462 156 88 4 1 155 1 149 1.86e-32 113
MsG0180001109.01.T01 MTR_8g045570 39.744 156 86 4 1 155 1 149 2.66e-30 108
MsG0180001109.01.T01 MTR_8g045490 38.462 156 88 4 1 155 1 149 1.03e-29 106
MsG0180001109.01.T01 MTR_8g045400 39.103 156 86 5 1 155 1 148 1.11e-29 106
MsG0180001109.01.T01 MTR_8g045695 39.103 156 86 5 1 155 1 148 3.58e-29 105
MsG0180001109.01.T01 MTR_8g045520 37.821 156 89 4 1 155 1 149 1.30e-28 103
MsG0180001109.01.T01 MTR_8g045735 37.821 156 88 5 1 155 1 148 5.74e-28 102
MsG0180001109.01.T01 MTR_8g045665 38.854 157 85 6 1 155 1 148 1.01e-27 101
MsG0180001109.01.T01 MTR_8g012550 36.601 153 90 2 6 158 8 153 1.07e-27 101
MsG0180001109.01.T01 MTR_8g045640 40.127 157 83 6 1 155 1 148 1.30e-27 101
MsG0180001109.01.T01 MTR_8g045560 38.854 157 85 6 1 155 1 148 5.94e-27 99.8
MsG0180001109.01.T01 MTR_8g045300 34.615 156 94 4 1 155 1 149 3.63e-23 89.7
MsG0180001109.01.T01 MTR_1g031640 31.081 148 89 5 9 155 9 144 2.52e-19 79.7
MsG0180001109.01.T01 MTR_5g035010 30.075 133 87 3 1 132 3 130 7.71e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001109.01.T01 AT1G70890 38.961 154 89 2 2 155 6 154 1.24e-34 119
MsG0180001109.01.T01 AT1G70840 38.312 154 90 2 2 155 19 167 7.92e-33 115
MsG0180001109.01.T01 AT1G70830 38.312 154 90 2 2 155 136 284 1.02e-31 115
MsG0180001109.01.T01 AT1G70830 35.294 102 61 2 60 161 32 128 2.18e-11 60.8
MsG0180001109.01.T01 AT1G70830 37.662 154 91 2 2 155 21 169 2.50e-31 111
MsG0180001109.01.T01 AT1G70830 37.500 160 95 2 2 161 21 175 2.91e-31 112
MsG0180001109.01.T01 AT1G70830 38.312 154 90 2 2 155 183 331 3.57e-31 115
MsG0180001109.01.T01 AT1G70830 37.500 160 95 2 2 161 21 175 6.03e-30 111
MsG0180001109.01.T01 AT1G70830 37.500 160 95 2 2 161 21 175 9.23e-31 112
MsG0180001109.01.T01 AT5G28010 37.662 154 91 2 2 155 14 162 9.41e-30 107
MsG0180001109.01.T01 AT5G28010 37.662 154 91 2 2 155 14 162 9.41e-30 107
MsG0180001109.01.T01 AT1G70850 36.364 154 93 2 2 155 8 156 1.50e-29 108
MsG0180001109.01.T01 AT1G70850 36.364 154 93 2 2 155 8 156 6.10e-29 108
MsG0180001109.01.T01 AT1G70850 37.762 143 84 2 13 155 175 312 1.77e-27 105
MsG0180001109.01.T01 AT1G70850 36.364 154 93 2 2 155 8 156 6.10e-29 108
MsG0180001109.01.T01 AT1G70850 37.762 143 84 2 13 155 175 312 1.77e-27 105
MsG0180001109.01.T01 AT1G70880 37.013 154 92 2 2 155 7 155 1.38e-28 103
MsG0180001109.01.T01 AT1G23130 31.818 154 99 3 2 155 9 156 6.39e-24 92.0
MsG0180001109.01.T01 AT5G28000 32.468 154 99 2 2 155 12 160 2.39e-22 88.2
MsG0180001109.01.T01 AT1G14930 32.258 155 98 3 1 155 1 148 2.50e-19 80.1
MsG0180001109.01.T01 AT1G35260 29.252 147 98 4 9 155 8 148 3.32e-17 74.3
MsG0180001109.01.T01 AT1G14950 29.747 158 98 4 1 155 1 148 1.32e-16 72.8
MsG0180001109.01.T01 AT1G14960 29.677 155 102 3 1 155 1 148 1.76e-16 72.4
MsG0180001109.01.T01 AT4G14060 30.380 158 97 4 1 155 1 148 3.05e-16 72.0
MsG0180001109.01.T01 AT1G30990 27.742 155 104 3 1 155 1 147 6.56e-16 70.9
MsG0180001109.01.T01 AT2G01520 29.114 158 99 4 1 155 1 148 7.56e-15 68.2
MsG0180001109.01.T01 AT1G35310 33.333 117 73 2 39 155 36 147 1.26e-14 67.8
MsG0180001109.01.T01 AT1G23120 28.767 146 93 4 10 155 10 144 6.52e-14 65.9
MsG0180001109.01.T01 AT2G01530 29.747 158 98 4 1 155 1 148 7.63e-14 65.5
MsG0180001109.01.T01 AT1G14940 28.387 155 104 3 1 155 1 148 1.29e-13 65.1
MsG0180001109.01.T01 AT1G23120 28.767 146 93 4 10 155 60 194 2.38e-13 65.1
MsG0180001109.01.T01 AT4G23670 30.380 158 97 4 1 155 1 148 1.68e-12 62.0
MsG0180001109.01.T01 AT1G24020 27.848 158 102 4 1 155 1 149 2.75e-12 61.6
MsG0180001109.01.T01 AT1G24020 27.848 158 102 4 1 155 1 149 2.75e-12 61.6
MsG0180001109.01.T01 AT4G23680 28.481 158 100 4 1 155 1 148 1.29e-11 59.7
MsG0180001109.01.T01 AT3G26460 24.051 158 108 3 1 155 1 149 1.93e-11 59.3
MsG0180001109.01.T01 AT3G26450 22.581 155 114 2 1 155 1 149 5.31e-11 58.2

Find 31 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAGAAACATGGCTTTAAA+TGG 0.277591 1:+16106266 None:intergenic
GAATTAGATATTAACGAATA+TGG 0.277926 1:+16107450 MsG0180001109.01.T01:CDS
GACTATAGATGATGAGAATA+AGG 0.344772 1:+16107347 MsG0180001109.01.T01:CDS
TTATCATCTTGAATTGGAAT+TGG 0.348432 1:+16107581 MsG0180001109.01.T01:CDS
ACTTGATCTGGGGAGATATC+AGG 0.362752 1:-16106368 None:intergenic
CTAGCTCCCTGTACTTGATC+TGG 0.371115 1:-16106380 None:intergenic
TATTGATTATCATCTTGAAT+TGG 0.376586 1:+16107575 MsG0180001109.01.T01:CDS
AAACTAAAGGAGGATATTAT+AGG 0.388937 1:+16107504 MsG0180001109.01.T01:CDS
ATCACATTTAGCCTCTTTGA+TGG 0.429050 1:+16107372 MsG0180001109.01.T01:CDS
ACAAGGTCCAAGTATAAATC+AGG 0.437514 1:-16107534 None:intergenic
ATTCACTGGGAATATACAAT+AGG 0.447928 1:+16106440 MsG0180001109.01.T01:CDS
ATGTTGGTTCTGTCATTCAC+TGG 0.454099 1:+16106426 MsG0180001109.01.T01:CDS
AAAATATTATAGACCTTAGC+AGG 0.463181 1:-16106323 None:intergenic
TGCATGTCCTGATTTATACT+TGG 0.469493 1:+16107527 MsG0180001109.01.T01:CDS
TCACATTTAGCCTCTTTGAT+GGG 0.477479 1:+16107373 MsG0180001109.01.T01:CDS
TAGCTCCCTGTACTTGATCT+GGG 0.481009 1:-16106379 None:intergenic
CTAGATTGCATGAAGGTGAC+TGG 0.500829 1:+16106399 MsG0180001109.01.T01:CDS
GAGAGTTACAACAGTTTCAA+AGG 0.505492 1:+16107405 MsG0180001109.01.T01:CDS
GATCAGAGCACTTCCTGCTA+AGG 0.539808 1:+16106310 MsG0180001109.01.T01:CDS
CACATTTAGCCTCTTTGATG+GGG 0.545910 1:+16107374 MsG0180001109.01.T01:CDS
ATACTTGGACCTTGTTGCAG+AGG 0.564386 1:+16107542 MsG0180001109.01.T01:CDS
TGTTGGTTCTGTCATTCACT+GGG 0.566404 1:+16106427 MsG0180001109.01.T01:CDS
TATCTCCCCAGATCAAGTAC+AGG 0.569400 1:+16106373 MsG0180001109.01.T01:CDS
AATATGGTTCACTTGTGAGA+TGG 0.591995 1:+16107466 MsG0180001109.01.T01:CDS
CACTCACATCCCCATCAAAG+AGG 0.593465 1:-16107383 None:intergenic
TAGATTGCATGAAGGTGACT+GGG 0.602773 1:+16106400 MsG0180001109.01.T01:CDS
AGCTCCCTGTACTTGATCTG+GGG 0.630841 1:-16106378 None:intergenic
GAAGGTGACTGGGAAAATGT+TGG 0.640506 1:+16106410 MsG0180001109.01.T01:CDS
ATCTCCCCAGATCAAGTACA+GGG 0.645330 1:+16106374 MsG0180001109.01.T01:CDS
CAGGGAGCTAGATTGCATGA+AGG 0.654119 1:+16106392 MsG0180001109.01.T01:CDS
TGAGTATGAGAAACTAAAGG+AGG 0.656053 1:+16107494 MsG0180001109.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTAATAGTTTTTATATTTA+AGG - Chr1:16106547-16106566 None:intergenic 10.0%
!! AATTATTACAAATATTTGGT+TGG + Chr1:16106667-16106686 MsG0180001109.01.T01:intron 15.0%
!! AGCTAATTATTACAAATATT+TGG + Chr1:16106663-16106682 MsG0180001109.01.T01:intron 15.0%
!! ATGAAAAATACTATATTTAG+TGG - Chr1:16107107-16107126 None:intergenic 15.0%
!!! AATAGTTTTTATATTTAAGG+AGG - Chr1:16106544-16106563 None:intergenic 15.0%
!!! ATAGTTTTTATATTTAAGGA+GGG - Chr1:16106543-16106562 None:intergenic 15.0%
!! GAATATACAATAGGTAATTA+AGG + Chr1:16106449-16106468 MsG0180001109.01.T01:intron 20.0%
!! GAATTAGATATTAACGAATA+TGG + Chr1:16107450-16107469 MsG0180001109.01.T01:CDS 20.0%
!! TCTCAATTAGAATAAAATAG+TGG + Chr1:16107032-16107051 MsG0180001109.01.T01:intron 20.0%
!!! TATTGATTATCATCTTGAAT+TGG + Chr1:16107575-16107594 MsG0180001109.01.T01:CDS 20.0%
! AAAATATTATAGACCTTAGC+AGG - Chr1:16106326-16106345 None:intergenic 25.0%
! AAACTAAAGGAGGATATTAT+AGG + Chr1:16107504-16107523 MsG0180001109.01.T01:CDS 25.0%
! TCTTATAACCGACAATAAAT+CGG - Chr1:16107008-16107027 None:intergenic 25.0%
! TGTATTATCCGATTTATTGT+CGG + Chr1:16106997-16107016 MsG0180001109.01.T01:intron 25.0%
! TTATCATCTTGAATTGGAAT+TGG + Chr1:16107581-16107600 MsG0180001109.01.T01:CDS 25.0%
!! CTAAGGTCTATAATATTTTC+AGG + Chr1:16106327-16106346 MsG0180001109.01.T01:CDS 25.0%
!!! TTATTTTTCTTCCAACTCAA+GGG + Chr1:16106731-16106750 MsG0180001109.01.T01:intron 25.0%
!!! TTTATTTTTCTTCCAACTCA+AGG + Chr1:16106730-16106749 MsG0180001109.01.T01:intron 25.0%
!!! TTTTGAGTATGAGAAACTAA+AGG + Chr1:16107491-16107510 MsG0180001109.01.T01:CDS 25.0%
ATCAAAGCATATTACAAGCA+AGG - Chr1:16106602-16106621 None:intergenic 30.0%
ATTCACTGGGAATATACAAT+AGG + Chr1:16106440-16106459 MsG0180001109.01.T01:CDS 30.0%
GACTATAGATGATGAGAATA+AGG + Chr1:16107347-16107366 MsG0180001109.01.T01:CDS 30.0%
TTACAAATATTTGGTTGGAG+AGG + Chr1:16106672-16106691 MsG0180001109.01.T01:intron 30.0%
! TTTCTTCATTTTCATCTGCA+GGG - Chr1:16107308-16107327 None:intergenic 30.0%
!! GACATTAACAAAAGAGTGTT+GGG - Chr1:16106580-16106599 None:intergenic 30.0%
AATATGGTTCACTTGTGAGA+TGG + Chr1:16107466-16107485 MsG0180001109.01.T01:CDS 35.0%
ACAAGGTCCAAGTATAAATC+AGG - Chr1:16107537-16107556 None:intergenic 35.0%
ATCACATTTAGCCTCTTTGA+TGG + Chr1:16107372-16107391 MsG0180001109.01.T01:CDS 35.0%
CAACTCAAGGGTTATAATGA+TGG + Chr1:16106743-16106762 MsG0180001109.01.T01:intron 35.0%
CATCATTATAACCCTTGAGT+TGG - Chr1:16106745-16106764 None:intergenic 35.0%
GAGAGTTACAACAGTTTCAA+AGG + Chr1:16107405-16107424 MsG0180001109.01.T01:CDS 35.0%
GGGTTATAATGATGGCAAAA+CGG + Chr1:16106751-16106770 MsG0180001109.01.T01:intron 35.0%
GGTTATAATGATGGCAAAAC+GGG + Chr1:16106752-16106771 MsG0180001109.01.T01:intron 35.0%
TCACATTTAGCCTCTTTGAT+GGG + Chr1:16107373-16107392 MsG0180001109.01.T01:CDS 35.0%
TGAGTATGAGAAACTAAAGG+AGG + Chr1:16107494-16107513 MsG0180001109.01.T01:CDS 35.0%
TGCATGTCCTGATTTATACT+TGG + Chr1:16107527-16107546 MsG0180001109.01.T01:CDS 35.0%
TTGAACCGATCACTAATACA+TGG + Chr1:16106831-16106850 MsG0180001109.01.T01:intron 35.0%
! CTTTCTTCATTTTCATCTGC+AGG - Chr1:16107309-16107328 None:intergenic 35.0%
! GGACATTAACAAAAGAGTGT+TGG - Chr1:16106581-16106600 None:intergenic 35.0%
! TCCAAATAAGTCTTGCTATG+CGG - Chr1:16106797-16106816 None:intergenic 35.0%
CACATTTAGCCTCTTTGATG+GGG + Chr1:16107374-16107393 MsG0180001109.01.T01:CDS 40.0%
GCATAGCAAGACTTATTTGG+AGG + Chr1:16106796-16106815 MsG0180001109.01.T01:intron 40.0%
GTGGACCATGTATTAGTGAT+CGG - Chr1:16106839-16106858 None:intergenic 40.0%
TAGATTGCATGAAGGTGACT+GGG + Chr1:16106400-16106419 MsG0180001109.01.T01:CDS 40.0%
TCCGCATAGCAAGACTTATT+TGG + Chr1:16106793-16106812 MsG0180001109.01.T01:intron 40.0%
TGGAGAGGTGATTAGAGTTT+GGG + Chr1:16106687-16106706 MsG0180001109.01.T01:intron 40.0%
TTGGAGAGGTGATTAGAGTT+TGG + Chr1:16106686-16106705 MsG0180001109.01.T01:intron 40.0%
! ACGGGTTATATTCGTCATGT+CGG + Chr1:16106770-16106789 MsG0180001109.01.T01:intron 40.0%
!! ATGTTGGTTCTGTCATTCAC+TGG + Chr1:16106426-16106445 MsG0180001109.01.T01:CDS 40.0%
!! TCGTTTTTAAGCAGGTTAGG+CGG - Chr1:16106863-16106882 None:intergenic 40.0%
!! TGTTGGTTCTGTCATTCACT+GGG + Chr1:16106427-16106446 MsG0180001109.01.T01:CDS 40.0%
AACCTGCTTAAAAACGAGGC+AGG + Chr1:16106866-16106885 MsG0180001109.01.T01:intron 45.0%
ACTTGATCTGGGGAGATATC+AGG - Chr1:16106371-16106390 None:intergenic 45.0%
AGAGGTGATTAGAGTTTGGG+AGG + Chr1:16106690-16106709 MsG0180001109.01.T01:intron 45.0%
ATACTTGGACCTTGTTGCAG+AGG + Chr1:16107542-16107561 MsG0180001109.01.T01:CDS 45.0%
ATCTCCCCAGATCAAGTACA+GGG + Chr1:16106374-16106393 MsG0180001109.01.T01:CDS 45.0%
CTAGATTGCATGAAGGTGAC+TGG + Chr1:16106399-16106418 MsG0180001109.01.T01:CDS 45.0%
GAAGGTGACTGGGAAAATGT+TGG + Chr1:16106410-16106429 MsG0180001109.01.T01:CDS 45.0%
GCCTAACCTGCTTAAAAACG+AGG + Chr1:16106862-16106881 MsG0180001109.01.T01:intron 45.0%
TAGCTCCCTGTACTTGATCT+GGG - Chr1:16106382-16106401 None:intergenic 45.0%
TATCTCCCCAGATCAAGTAC+AGG + Chr1:16106373-16106392 MsG0180001109.01.T01:CDS 45.0%
TGCTTAAAAACGAGGCAGGA+CGG + Chr1:16106870-16106889 MsG0180001109.01.T01:intron 45.0%
! CTTTTGTGACCTCTGCAACA+AGG - Chr1:16107554-16107573 None:intergenic 45.0%
!! CGTTTTTAAGCAGGTTAGGC+GGG - Chr1:16106862-16106881 None:intergenic 45.0%
!! GCCTCGTTTTTAAGCAGGTT+AGG - Chr1:16106866-16106885 None:intergenic 45.0%
!! GTTTTTAAGCAGGTTAGGCG+GGG - Chr1:16106861-16106880 None:intergenic 45.0%
!! ATATTAATAAAATGTATTTA+AGG + Chr1:16107073-16107092 MsG0180001109.01.T01:intron 5.0%
!! TATTAATAAAATGTATTTAA+GGG + Chr1:16107074-16107093 MsG0180001109.01.T01:intron 5.0%
!!! AATAAAATAAAAATTCTTTT+TGG + Chr1:16106911-16106930 MsG0180001109.01.T01:intron 5.0%
AGCTCCCTGTACTTGATCTG+GGG - Chr1:16106381-16106400 None:intergenic 50.0%
CACTCACATCCCCATCAAAG+AGG - Chr1:16107386-16107405 None:intergenic 50.0%
CAGGGAGCTAGATTGCATGA+AGG + Chr1:16106392-16106411 MsG0180001109.01.T01:CDS 50.0%
CTAGCTCCCTGTACTTGATC+TGG - Chr1:16106383-16106402 None:intergenic 50.0%
! GATCAGAGCACTTCCTGCTA+AGG + Chr1:16106310-16106329 MsG0180001109.01.T01:CDS 50.0%
!! GTCCTGCCTCGTTTTTAAGC+AGG - Chr1:16106871-16106890 None:intergenic 50.0%
TTTAAGCAGGTTAGGCGGGG+TGG - Chr1:16106858-16106877 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 16106275 16107623 16106275 ID=MsG0180001109.01;Name=MsG0180001109.01
Chr1 mRNA 16106275 16107623 16106275 ID=MsG0180001109.01.T01;Parent=MsG0180001109.01;Name=MsG0180001109.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|2|0|165
Chr1 exon 16106275 16106461 16106275 ID=MsG0180001109.01.T01:exon:31198;Parent=MsG0180001109.01.T01
Chr1 exon 16107313 16107623 16107313 ID=MsG0180001109.01.T01:exon:31199;Parent=MsG0180001109.01.T01
Chr1 CDS 16106275 16106461 16106275 ID=MsG0180001109.01.T01:cds;Parent=MsG0180001109.01.T01
Chr1 CDS 16107313 16107623 16107313 ID=MsG0180001109.01.T01:cds;Parent=MsG0180001109.01.T01
Gene Sequence

>MsG0180001109.01.T01

ATGGCTTTAAATGGAAAAGTGTTCACTGAAATAGAGATCAGAGCACTTCCTGCTAAGGTCTATAATATTTTCAGGAAGCAACTTAAACAAATTCCTGATATCTCCCCAGATCAAGTACAGGGAGCTAGATTGCATGAAGGTGACTGGGAAAATGTTGGTTCTGTCATTCACTGGGAATATACAATAGATGAAAATGAAGAAAGTGCTAAAGTTAAAATTGAGACTATAGATGATGAGAATAAGGTAATCACATTTAGCCTCTTTGATGGGGATGTGAGTGAGAGTTACAACAGTTTCAAAGGAACATTAGAAGTGTTCGATTGCGAATTAGATATTAACGAATATGGTTCACTTGTGAGATGGACTTTTGAGTATGAGAAACTAAAGGAGGATATTATAGGTGCATGTCCTGATTTATACTTGGACCTTGTTGCAGAGGTCACAAAAGATATTGATTATCATCTTGAATTGGAATTGGAACAAGATGAACAAAGATGA

Protein sequence

>MsG0180001109.01.T01

MALNGKVFTEIEIRALPAKVYNIFRKQLKQIPDISPDQVQGARLHEGDWENVGSVIHWEYTIDENEESAKVKIETIDDENKVITFSLFDGDVSESYNSFKGTLEVFDCELDINEYGSLVRWTFEYEKLKEDIIGACPDLYLDLVAEVTKDIDYHLELELEQDEQR*