AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025828.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025828.01.T01 MTR_5g035010 94.231 156 9 0 1 156 1 156 3.52e-111 312
MsG0580025828.01.T01 MTR_8g012550 29.139 151 106 1 1 151 1 150 4.01e-19 79.0
MsG0580025828.01.T01 MTR_1g030810 31.373 153 98 5 3 151 1 150 7.73e-15 67.8
MsG0580025828.01.T01 MTR_1g030820 30.323 155 97 6 3 151 1 150 1.25e-13 64.7
MsG0580025828.01.T01 MTR_1g031640 29.630 135 86 5 9 141 7 134 1.66e-12 61.6
MsG0580025828.01.T01 MTR_1g030840 31.852 135 82 4 3 130 1 132 2.01e-12 61.6
MsG0580025828.01.T01 MTR_8g045520 26.452 155 110 4 3 155 1 153 1.67e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025828.01.T01 AT1G24020 51.678 149 72 0 3 151 1 149 2.75e-55 171
MsG0580025828.01.T01 AT1G24020 51.678 149 72 0 3 151 1 149 2.75e-55 171
MsG0580025828.01.T01 AT1G70830 34.868 152 96 2 2 151 19 169 1.43e-22 88.6
MsG0580025828.01.T01 AT1G70840 36.667 150 92 2 4 151 19 167 2.41e-22 87.8
MsG0580025828.01.T01 AT1G70830 34.868 152 96 2 2 151 19 169 2.74e-22 88.6
MsG0580025828.01.T01 AT1G70830 34.868 152 96 2 2 151 19 169 9.26e-22 88.2
MsG0580025828.01.T01 AT1G70850 34.416 154 98 2 2 153 6 158 1.47e-21 87.4
MsG0580025828.01.T01 AT1G70830 34.868 152 96 2 2 151 19 169 2.29e-21 88.6
MsG0580025828.01.T01 AT1G70830 33.333 150 97 2 4 151 183 331 1.34e-19 84.0
MsG0580025828.01.T01 AT1G70850 34.416 154 98 2 2 153 6 158 4.38e-21 87.4
MsG0580025828.01.T01 AT1G70850 33.813 139 89 2 15 151 175 312 1.60e-17 77.8
MsG0580025828.01.T01 AT1G70850 34.416 154 98 2 2 153 6 158 4.38e-21 87.4
MsG0580025828.01.T01 AT1G70850 33.813 139 89 2 15 151 175 312 1.60e-17 77.8
MsG0580025828.01.T01 AT1G70890 35.526 152 95 3 2 151 4 154 2.35e-20 82.4
MsG0580025828.01.T01 AT5G28010 32.237 152 100 2 4 153 14 164 5.38e-20 81.6
MsG0580025828.01.T01 AT5G28010 32.237 152 100 2 4 153 14 164 5.38e-20 81.6
MsG0580025828.01.T01 AT1G70830 33.333 150 97 2 4 151 136 284 6.65e-20 84.0
MsG0580025828.01.T01 AT5G28000 31.579 152 101 2 4 153 12 162 1.60e-17 75.1
MsG0580025828.01.T01 AT1G70880 31.169 154 103 3 2 153 5 157 3.57e-17 74.3
MsG0580025828.01.T01 AT1G14930 27.815 151 104 3 3 151 1 148 1.24e-13 64.7
MsG0580025828.01.T01 AT1G35260 31.081 148 96 4 9 153 6 150 1.63e-13 64.3
MsG0580025828.01.T01 AT1G23120 28.571 147 91 5 9 154 7 140 1.22e-11 59.3
MsG0580025828.01.T01 AT1G23120 28.571 154 96 5 2 154 50 190 2.60e-11 59.3
MsG0580025828.01.T01 AT3G26460 25.333 150 110 2 3 151 1 149 8.30e-11 57.4

Find 44 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGATCACATTAAATAAAA+AGG 0.223692 5:+24829394 None:intergenic
CTTTGTTCCTTTGTTATTGT+TGG 0.271015 5:-24829360 MsG0580025828.01.T01:three_prime_UTR
AAGCTAGAAAGAAATTAAAT+TGG 0.308506 5:+24830189 None:intergenic
TAGTCATGTGGGAAGGCCTT+AGG 0.333006 5:+24830059 None:intergenic
AATAGTTGTAGAATCCCTTA+TGG 0.338562 5:+24830085 None:intergenic
GCAAAATCTTTGACAATAGT+TGG 0.338740 5:+24829559 None:intergenic
CTTTCTAGCTTATTGAATCA+TGG 0.378434 5:-24830177 MsG0580025828.01.T01:exon
ATCCCCTTCAATTATACAAT+AGG 0.381348 5:+24829696 None:intergenic
ATCTCACATCCTTCTCCAAC+TGG 0.381866 5:+24829628 None:intergenic
TGATTTAAATTTAATGAGAA+TGG 0.383658 5:-24829299 MsG0580025828.01.T01:three_prime_UTR
CCTTGAATGATTTGTAGTAT+TGG 0.436050 5:+24829668 None:intergenic
CCAATACTACAAATCATTCA+AGG 0.446219 5:-24829668 MsG0580025828.01.T01:CDS
AGTCTAATGCAGACAAGTAC+TGG 0.454231 5:-24830112 MsG0580025828.01.T01:CDS
AGTCATGTGGGAAGGCCTTA+GGG 0.459268 5:+24830060 None:intergenic
TTAAATCATAACAATTAAAG+AGG 0.466331 5:+24829314 None:intergenic
GAGAAGGATGTGAGATGAAA+TGG 0.470943 5:-24829621 MsG0580025828.01.T01:CDS
TGCATTTGATGATGAGTTGA+AGG 0.473972 5:-24829474 MsG0580025828.01.T01:three_prime_UTR
ACAAGTACTGGCAAACCATA+AGG 0.476885 5:-24830100 MsG0580025828.01.T01:CDS
GAGGGTGATGGTAAAGCTCC+TGG 0.482745 5:-24830017 MsG0580025828.01.T01:CDS
TCTTTGACAATAGTTGGATC+AGG 0.487708 5:+24829565 None:intergenic
GTGAAGTGGCGGATAGAGCC+AGG 0.488549 5:+24829999 None:intergenic
GCAACCTATTGTATAATTGA+AGG 0.505768 5:-24829700 MsG0580025828.01.T01:CDS
AAAAGCATTGAGGTCCTTGA+GGG 0.507266 5:-24830035 MsG0580025828.01.T01:CDS
CAAGTACTGGCAAACCATAA+GGG 0.509772 5:-24830099 MsG0580025828.01.T01:CDS
AAATGTATTGTTACATAATG+TGG 0.517276 5:-24829429 MsG0580025828.01.T01:three_prime_UTR
ATCATGGCTGTAGCTCTTCG+TGG 0.517791 5:-24830161 MsG0580025828.01.T01:CDS
CAACCTATTGTATAATTGAA+GGG 0.524179 5:-24829699 MsG0580025828.01.T01:CDS
TACCTTCAGCATAAGTGAAG+TGG 0.548909 5:+24829985 None:intergenic
TGCAGTCAAGAACTTCATTG+AGG 0.557320 5:-24829539 MsG0580025828.01.T01:CDS
CAAAAGCATTGAGGTCCTTG+AGG 0.558857 5:-24830036 MsG0580025828.01.T01:CDS
CGCCACTTCACTTATGCTGA+AGG 0.559188 5:-24829987 MsG0580025828.01.T01:intron
ATTGAGGTCCTTGAGGGTGA+TGG 0.560374 5:-24830029 MsG0580025828.01.T01:CDS
CATCTTGCTGTAACACCAGT+TGG 0.560972 5:-24829643 MsG0580025828.01.T01:CDS
CAATACTACAAATCATTCAA+GGG 0.584423 5:-24829667 MsG0580025828.01.T01:CDS
GCTGTAACACCAGTTGGAGA+AGG 0.585512 5:-24829637 MsG0580025828.01.T01:CDS
AACCTATTGTATAATTGAAG+GGG 0.589185 5:-24829698 MsG0580025828.01.T01:CDS
GAGCTTTACCATCACCCTCA+AGG 0.617751 5:+24830021 None:intergenic
ATGCTGCTGATGATGAAAAG+AGG 0.618271 5:-24829726 MsG0580025828.01.T01:CDS
AGTCAAGAACTTCATTGAGG+TGG 0.628959 5:-24829536 MsG0580025828.01.T01:CDS
CATTAAGCCAACAATAACAA+AGG 0.631117 5:+24829353 None:intergenic
CTTCAGCATAAGTGAAGTGG+CGG 0.638511 5:+24829988 None:intergenic
ACAACTAGCGAAGTCTTCGA+CGG 0.642685 5:-24829758 MsG0580025828.01.T01:CDS
AGCTCTTCGTGGAAAGCTAG+AGG 0.660101 5:-24830150 MsG0580025828.01.T01:CDS
ACATGACTACAAAAGCATTG+AGG 0.674108 5:-24830045 MsG0580025828.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGATCACATTAAATAAAA+AGG + Chr5:24830140-24830159 None:intergenic 15.0%
!! TAGATCACATTAAATAAAAA+GGG + Chr5:24830139-24830158 None:intergenic 15.0%
!! TGATTTAAATTTAATGAGAA+TGG - Chr5:24830232-24830251 MsG0580025828.01.T01:five_prime_UTR 15.0%
!! TTAAATCATAACAATTAAAG+AGG + Chr5:24830220-24830239 None:intergenic 15.0%
!! AAATGTATTGTTACATAATG+TGG - Chr5:24830102-24830121 MsG0580025828.01.T01:CDS 20.0%
!! AAGCTAGAAAGAAATTAAAT+TGG + Chr5:24829345-24829364 None:intergenic 20.0%
! AAAGTGAGTATTATGTATGT+CGG + Chr5:24829674-24829693 None:intergenic 25.0%
! AACCTATTGTATAATTGAAG+GGG - Chr5:24829833-24829852 MsG0580025828.01.T01:intron 25.0%
! AATTTAGAGACTTTCTTCTT+TGG - Chr5:24829570-24829589 MsG0580025828.01.T01:CDS 25.0%
! CAACCTATTGTATAATTGAA+GGG - Chr5:24829832-24829851 MsG0580025828.01.T01:intron 25.0%
! CAATACTACAAATCATTCAA+GGG - Chr5:24829864-24829883 MsG0580025828.01.T01:intron 25.0%
! GTGATCCTACAATATATTAT+AGG + Chr5:24829755-24829774 None:intergenic 25.0%
AATAGTTGTAGAATCCCTTA+TGG + Chr5:24829449-24829468 None:intergenic 30.0%
ACACAAGTTCTTATCTGATT+TGG - Chr5:24829726-24829745 MsG0580025828.01.T01:CDS 30.0%
ATCCCCTTCAATTATACAAT+AGG + Chr5:24829838-24829857 None:intergenic 30.0%
CATTAAGCCAACAATAACAA+AGG + Chr5:24830181-24830200 None:intergenic 30.0%
CCAATACTACAAATCATTCA+AGG - Chr5:24829863-24829882 MsG0580025828.01.T01:intron 30.0%
GCAAAATCTTTGACAATAGT+TGG + Chr5:24829975-24829994 None:intergenic 30.0%
GCAACCTATTGTATAATTGA+AGG - Chr5:24829831-24829850 MsG0580025828.01.T01:intron 30.0%
! CCTTGAATGATTTGTAGTAT+TGG + Chr5:24829866-24829885 None:intergenic 30.0%
! TTCTACAACTATTTTCCCTA+AGG - Chr5:24829456-24829475 MsG0580025828.01.T01:three_prime_UTR 30.0%
!! CTTTCTAGCTTATTGAATCA+TGG - Chr5:24829354-24829373 MsG0580025828.01.T01:three_prime_UTR 30.0%
!! CTTTGTTCCTTTGTTATTGT+TGG - Chr5:24830171-24830190 MsG0580025828.01.T01:exon 30.0%
CTTCTTTGGTAGAGAATGAA+GGG - Chr5:24829584-24829603 MsG0580025828.01.T01:CDS 35.0%
GGTGGCCTATAATATATTGT+AGG - Chr5:24829747-24829766 MsG0580025828.01.T01:CDS 35.0%
GTCAAATGCAACGAATATTG+TGG + Chr5:24829700-24829719 None:intergenic 35.0%
TCTTCTTTGGTAGAGAATGA+AGG - Chr5:24829583-24829602 MsG0580025828.01.T01:CDS 35.0%
TCTTTGACAATAGTTGGATC+AGG + Chr5:24829969-24829988 None:intergenic 35.0%
! CAATGCTTTTGTAGTCATGT+GGG + Chr5:24829486-24829505 None:intergenic 35.0%
! TCAATGCTTTTGTAGTCATG+TGG + Chr5:24829487-24829506 None:intergenic 35.0%
!! ACATGACTACAAAAGCATTG+AGG - Chr5:24829486-24829505 MsG0580025828.01.T01:three_prime_UTR 35.0%
!! CAAGTTCTTATCTGATTTGG+TGG - Chr5:24829729-24829748 MsG0580025828.01.T01:CDS 35.0%
!! TGCATTTGATGATGAGTTGA+AGG - Chr5:24830057-24830076 MsG0580025828.01.T01:CDS 35.0%
!!! TAGAGAATGAAGGGCATTTT+GGG - Chr5:24829593-24829612 MsG0580025828.01.T01:CDS 35.0%
AGTCAAGAACTTCATTGAGG+TGG - Chr5:24829995-24830014 MsG0580025828.01.T01:CDS 40.0%
AGTCTAATGCAGACAAGTAC+TGG - Chr5:24829419-24829438 MsG0580025828.01.T01:three_prime_UTR 40.0%
GAGAAGGATGTGAGATGAAA+TGG - Chr5:24829910-24829929 MsG0580025828.01.T01:intron 40.0%
TACCTTCAGCATAAGTGAAG+TGG + Chr5:24829549-24829568 None:intergenic 40.0%
TGCAGTCAAGAACTTCATTG+AGG - Chr5:24829992-24830011 MsG0580025828.01.T01:CDS 40.0%
! ATGCTGCTGATGATGAAAAG+AGG - Chr5:24829805-24829824 MsG0580025828.01.T01:intron 40.0%
!! AAAAGCATTGAGGTCCTTGA+GGG - Chr5:24829496-24829515 MsG0580025828.01.T01:exon 40.0%
!! ACAAGTACTGGCAAACCATA+AGG - Chr5:24829431-24829450 MsG0580025828.01.T01:three_prime_UTR 40.0%
!! CAAGTACTGGCAAACCATAA+GGG - Chr5:24829432-24829451 MsG0580025828.01.T01:three_prime_UTR 40.0%
!!! GTAGAGAATGAAGGGCATTT+TGG - Chr5:24829592-24829611 MsG0580025828.01.T01:CDS 40.0%
ACAACTAGCGAAGTCTTCGA+CGG - Chr5:24829773-24829792 MsG0580025828.01.T01:intron 45.0%
ATCTCACATCCTTCTCCAAC+TGG + Chr5:24829906-24829925 None:intergenic 45.0%
CTTCAGCATAAGTGAAGTGG+CGG + Chr5:24829546-24829565 None:intergenic 45.0%
! CATCTTGCTGTAACACCAGT+TGG - Chr5:24829888-24829907 MsG0580025828.01.T01:intron 45.0%
! GCTTTTGTAGTCATGTGGGA+AGG + Chr5:24829482-24829501 None:intergenic 45.0%
!! CAAAAGCATTGAGGTCCTTG+AGG - Chr5:24829495-24829514 MsG0580025828.01.T01:exon 45.0%
AGCTCTTCGTGGAAAGCTAG+AGG - Chr5:24829381-24829400 MsG0580025828.01.T01:three_prime_UTR 50.0%
AGTCATGTGGGAAGGCCTTA+GGG + Chr5:24829474-24829493 None:intergenic 50.0%
ATCATGGCTGTAGCTCTTCG+TGG - Chr5:24829370-24829389 MsG0580025828.01.T01:three_prime_UTR 50.0%
ATTGAGGTCCTTGAGGGTGA+TGG - Chr5:24829502-24829521 MsG0580025828.01.T01:exon 50.0%
CGCCACTTCACTTATGCTGA+AGG - Chr5:24829544-24829563 MsG0580025828.01.T01:CDS 50.0%
GAGCTTTACCATCACCCTCA+AGG + Chr5:24829513-24829532 None:intergenic 50.0%
GCTGTAACACCAGTTGGAGA+AGG - Chr5:24829894-24829913 MsG0580025828.01.T01:intron 50.0%
TAGTCATGTGGGAAGGCCTT+AGG + Chr5:24829475-24829494 None:intergenic 50.0%
! GAGGGTGATGGTAAAGCTCC+TGG - Chr5:24829514-24829533 MsG0580025828.01.T01:CDS 55.0%
!! GTGAAGTGGCGGATAGAGCC+AGG + Chr5:24829535-24829554 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 24829284 24830269 24829284 ID=MsG0580025828.01;Name=MsG0580025828.01
Chr5 mRNA 24829284 24830269 24829284 ID=MsG0580025828.01.T01;Parent=MsG0580025828.01;Name=MsG0580025828.01.T01;_AED=0.49;_eAED=0.49;_QI=89|1|1|1|1|1|2|223|156
Chr5 exon 24829284 24829784 24829284 ID=MsG0580025828.01.T01:exon:3997;Parent=MsG0580025828.01.T01
Chr5 exon 24829988 24830269 24829988 ID=MsG0580025828.01.T01:exon:3996;Parent=MsG0580025828.01.T01
Chr5 five_prime_UTR 24830181 24830269 24830181 ID=MsG0580025828.01.T01:five_prime_utr;Parent=MsG0580025828.01.T01
Chr5 CDS 24829988 24830180 24829988 ID=MsG0580025828.01.T01:cds;Parent=MsG0580025828.01.T01
Chr5 CDS 24829507 24829784 24829507 ID=MsG0580025828.01.T01:cds;Parent=MsG0580025828.01.T01
Chr5 three_prime_UTR 24829284 24829506 24829284 ID=MsG0580025828.01.T01:three_prime_utr;Parent=MsG0580025828.01.T01
Gene Sequence

>MsG0580025828.01.T01

ATGGCTGTAGCTCTTCGTGGAAAGCTAGAGGTAGACATTGAACTCAAGTCTAATGCAGACAAGTACTGGCAAACCATAAGGGATTCTACAACTATTTTCCCTAAGGCCTTCCCACATGACTACAAAAGCATTGAGGTCCTTGAGGGTGATGGTAAAGCTCCTGGCTCTATCCGCCACTTCACTTATGCTGAAGGATCACAACTAGCGAAGTCTTCGACGGAGAAGATTGATGCTGCTGATGATGAAAAGAGGACAGCAACCTATTGTATAATTGAAGGGGATCTTCTCCAATACTACAAATCATTCAAGGGACATCTTGCTGTAACACCAGTTGGAGAAGGATGTGAGATGAAATGGTGTGCTGAGTATATTAAAGTTAGTAATGATATTCCTGATCCAACTATTGTCAAAGATTTTGCAGTCAAGAACTTCATTGAGGTGGATGACTATGTTCAATCACTTGCTGCATAG

Protein sequence

>MsG0580025828.01.T01

MAVALRGKLEVDIELKSNADKYWQTIRDSTTIFPKAFPHDYKSIEVLEGDGKAPGSIRHFTYAEGSQLAKSSTEKIDAADDEKRTATYCIIEGDLLQYYKSFKGHLAVTPVGEGCEMKWCAEYIKVSNDIPDPTIVKDFAVKNFIEVDDYVQSLAA*