AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048633.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048633.01.T01 MTR_8g045490 92.308 156 12 0 1 156 1 156 1.65e-105 298
MsG0080048633.01.T01 MTR_8g045520 81.529 157 28 1 1 156 1 157 5.68e-93 266
MsG0080048633.01.T01 MTR_8g045400 78.344 157 32 2 1 156 1 156 3.21e-88 254
MsG0080048633.01.T01 MTR_8g045570 76.316 152 36 0 1 152 1 152 1.81e-86 250
MsG0080048633.01.T01 MTR_8g045695 74.510 153 38 1 1 153 1 152 1.06e-83 243
MsG0080048633.01.T01 MTR_8g045555 75.000 152 38 0 1 152 1 152 1.47e-83 242
MsG0080048633.01.T01 MTR_8g045300 69.872 156 46 1 1 155 1 156 1.22e-80 235
MsG0080048633.01.T01 MTR_8g045735 70.588 153 44 1 1 153 1 152 7.30e-80 233
MsG0080048633.01.T01 MTR_8g045665 70.588 153 44 1 1 153 1 152 5.13e-78 228
MsG0080048633.01.T01 MTR_8g045640 69.281 153 46 1 1 153 1 152 5.90e-76 223
MsG0080048633.01.T01 MTR_8g045560 67.974 153 48 1 1 153 1 152 1.16e-74 220
MsG0080048633.01.T01 MTR_1g030810 43.137 153 84 2 1 151 1 152 1.33e-40 134
MsG0080048633.01.T01 MTR_1g030820 40.523 153 88 2 1 151 1 152 3.04e-40 133
MsG0080048633.01.T01 MTR_1g030840 37.179 156 90 4 1 149 1 155 4.74e-29 104
MsG0080048633.01.T01 MTR_8g012550 35.616 146 91 3 6 150 8 151 1.62e-28 103
MsG0080048633.01.T01 MTR_1g031640 38.514 148 82 5 7 152 7 147 5.38e-26 96.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048633.01.T01 AT5G28010 36.242 149 92 3 3 149 15 162 7.26e-27 99.4
MsG0080048633.01.T01 AT5G28010 36.242 149 92 3 3 149 15 162 7.26e-27 99.4
MsG0080048633.01.T01 AT1G70890 37.584 149 90 3 3 149 7 154 2.79e-26 97.8
MsG0080048633.01.T01 AT1G70830 34.899 149 94 3 3 149 22 169 5.48e-24 92.0
MsG0080048633.01.T01 AT1G70830 34.899 149 94 3 3 149 22 169 1.02e-23 92.4
MsG0080048633.01.T01 AT1G70840 32.886 149 97 3 3 149 20 167 2.33e-23 90.5
MsG0080048633.01.T01 AT1G70830 34.899 149 94 3 3 149 22 169 3.60e-23 92.0
MsG0080048633.01.T01 AT5G28000 32.886 149 97 3 3 149 13 160 9.61e-23 88.6
MsG0080048633.01.T01 AT1G70830 34.899 149 94 3 3 149 22 169 9.91e-23 92.4
MsG0080048633.01.T01 AT1G70830 33.557 149 96 3 3 149 184 331 3.60e-21 88.2
MsG0080048633.01.T01 AT1G70870 35.948 153 81 4 1 151 1 138 8.70e-22 85.5
MsG0080048633.01.T01 AT1G70830 33.557 149 96 3 3 149 137 284 1.31e-21 88.6
MsG0080048633.01.T01 AT1G70830 30.435 92 63 1 59 149 31 122 6.47e-11 59.3
MsG0080048633.01.T01 AT1G70850 32.886 149 97 3 3 149 9 156 1.67e-21 87.4
MsG0080048633.01.T01 AT1G23120 30.769 143 94 3 7 149 7 144 4.42e-21 84.0
MsG0080048633.01.T01 AT1G70850 32.886 149 97 3 3 149 9 156 4.70e-21 87.4
MsG0080048633.01.T01 AT1G70850 33.094 139 90 3 13 149 175 312 9.04e-20 84.0
MsG0080048633.01.T01 AT1G70850 32.886 149 97 3 3 149 9 156 4.70e-21 87.4
MsG0080048633.01.T01 AT1G70850 33.094 139 90 3 13 149 175 312 9.04e-20 84.0
MsG0080048633.01.T01 AT4G23670 34.641 153 97 3 1 152 1 151 7.55e-21 83.6
MsG0080048633.01.T01 AT1G23130 30.405 148 101 2 3 149 10 156 1.21e-20 83.2
MsG0080048633.01.T01 AT1G23120 30.769 143 94 3 7 149 57 194 1.30e-20 84.0
MsG0080048633.01.T01 AT1G70880 30.872 149 100 3 3 149 8 155 5.31e-20 81.6
MsG0080048633.01.T01 AT2G01520 32.026 153 101 3 1 152 1 151 7.42e-19 78.2
MsG0080048633.01.T01 AT4G23680 32.026 153 101 3 1 152 1 151 2.91e-18 76.6
MsG0080048633.01.T01 AT1G14930 32.298 161 92 5 1 153 1 152 1.15e-17 75.5
MsG0080048633.01.T01 AT2G01530 32.026 153 101 3 1 152 1 151 2.26e-17 74.3
MsG0080048633.01.T01 AT4G14060 32.680 153 100 3 1 152 1 151 2.86e-17 74.3
MsG0080048633.01.T01 AT1G35260 28.966 145 99 3 7 149 6 148 3.74e-17 73.9
MsG0080048633.01.T01 AT1G24020 32.692 156 103 2 1 155 1 155 5.09e-16 71.2
MsG0080048633.01.T01 AT1G24020 32.692 156 103 2 1 155 1 155 5.09e-16 71.2
MsG0080048633.01.T01 AT1G30990 32.000 150 98 4 1 149 1 147 2.18e-15 69.3
MsG0080048633.01.T01 AT1G14940 30.000 150 102 3 1 149 1 148 3.75e-14 66.2
MsG0080048633.01.T01 AT3G26460 32.051 156 98 5 1 152 1 152 8.39e-14 65.1
MsG0080048633.01.T01 AT1G14950 28.947 152 105 3 1 151 1 150 1.37e-13 64.7
MsG0080048633.01.T01 AT1G35310 25.362 138 100 3 13 149 12 147 2.00e-12 61.6
MsG0080048633.01.T01 AT1G14960 28.571 154 107 3 1 153 1 152 8.48e-12 60.1
MsG0080048633.01.T01 AT3G26450 27.273 154 108 4 1 152 1 152 2.95e-11 58.5

Find 41 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGCACTGCTGCTGCTAAA+TGG 0.310932 contig380end:+8914 MsG0080048633.01.T01:CDS
CTTCCCTTTCTTCTAAGAAA+TGG 0.349077 contig380end:+7791 MsG0080048633.01.T01:exon
TATGTGATGAAATCAATAAA+TGG 0.369456 contig380end:+9247 MsG0080048633.01.T01:three_prime_UTR
CACCCTTATCAATGACTTCA+AGG 0.380806 contig380end:-8891 None:intergenic
TTATAGAGCTATCTTTGCTT+TGG 0.392094 contig380end:-9034 None:intergenic
AATTAGTGTGATATTTGTAA+TGG 0.409729 contig380end:+9131 MsG0080048633.01.T01:three_prime_UTR
ATATTGTTGCATATGCATAT+TGG 0.412178 contig380end:+9155 MsG0080048633.01.T01:three_prime_UTR
TAGTAATGGGTTATTGCATT+GGG 0.416848 contig380end:+9081 MsG0080048633.01.T01:three_prime_UTR
TGTAACAAATAACTAGTAAT+GGG 0.422186 contig380end:+9068 MsG0080048633.01.T01:three_prime_UTR
AGCACCATTTCTTAGAAGAA+AGG 0.435379 contig380end:-7795 None:intergenic
AAACACTGGACTTACGTCAT+AGG 0.436108 contig380end:+7975 MsG0080048633.01.T01:CDS
TTCTAAGAAATGGTGCTCTC+TGG 0.436928 contig380end:+7801 MsG0080048633.01.T01:exon
CATCCTTGAAGTCATTGATA+AGG 0.445764 contig380end:+8888 MsG0080048633.01.T01:CDS
AAGTATTCCATGTAGCCATT+TGG 0.448595 contig380end:-8976 None:intergenic
CTTCTATGTGATGTTTGTAA+TGG 0.450639 contig380end:+9178 MsG0080048633.01.T01:three_prime_UTR
CTAGTAATGGGTTATTGCAT+TGG 0.451009 contig380end:+9080 MsG0080048633.01.T01:three_prime_UTR
AACACTTATAAGCTCTTTGG+TGG 0.457999 contig380end:+8832 MsG0080048633.01.T01:CDS
GCACCATTTCTTAGAAGAAA+GGG 0.460369 contig380end:-7794 None:intergenic
ATGTAACAAATAACTAGTAA+TGG 0.468583 contig380end:+9067 MsG0080048633.01.T01:three_prime_UTR
AACTTCATGAAGGTGAAGAT+TGG 0.478377 contig380end:+7934 MsG0080048633.01.T01:CDS
GGTAAGCTCAGTACTGAAGT+TGG 0.490802 contig380end:+7822 MsG0080048633.01.T01:CDS
AATGGGTTATTGCATTGGGT+TGG 0.493837 contig380end:+9085 MsG0080048633.01.T01:three_prime_UTR
ATCCTTGAAGTCATTGATAA+GGG 0.494628 contig380end:+8889 MsG0080048633.01.T01:CDS
AGATATTGATGAGCACTATA+AGG 0.500590 contig380end:+8855 MsG0080048633.01.T01:CDS
GAACAGATTGTAGAACTTGT+CGG 0.501167 contig380end:-7859 None:intergenic
TATTCCATGTAGCCATTTGG+AGG 0.517921 contig380end:-8973 None:intergenic
AGATTGGCATCACACTGATT+CGG 0.519723 contig380end:+7950 MsG0080048633.01.T01:CDS
AATGGCTACATGGAATACTT+TGG 0.525683 contig380end:+8979 MsG0080048633.01.T01:CDS
TGACCCTCCAAATGGCTACA+TGG 0.526835 contig380end:+8969 MsG0080048633.01.T01:CDS
GAGGATATTGACCCTCCAAA+TGG 0.540200 contig380end:+8961 MsG0080048633.01.T01:CDS
TATGATGAACTCTTTCACAA+AGG 0.552779 contig380end:-7908 None:intergenic
ACACTTTCATCAACTCACAT+AGG 0.573859 contig380end:+7750 None:intergenic
GAAGTCATTGATAAGGGTGA+TGG 0.579318 contig380end:+8895 MsG0080048633.01.T01:CDS
ATCATAATTCACAGATGGCA+AGG 0.582682 contig380end:+8759 MsG0080048633.01.T01:intron
CATCATACAAAACTTCATGA+AGG 0.606616 contig380end:+7924 MsG0080048633.01.T01:CDS
ACACTGATTCGGTTAAACAC+TGG 0.614381 contig380end:+7961 MsG0080048633.01.T01:CDS
ATTCCATGTAGCCATTTGGA+GGG 0.625101 contig380end:-8972 None:intergenic
AGATTGTAGAACTTGTCGGC+GGG 0.646397 contig380end:-7855 None:intergenic
TGAATATGAAAAGATCAATG+AGG 0.667026 contig380end:+8942 MsG0080048633.01.T01:CDS
CAGATTGTAGAACTTGTCGG+CGG 0.688816 contig380end:-7856 None:intergenic
AGTGTGATGTCTGTACATGA+CGG 0.731855 contig380end:+9205 MsG0080048633.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGAATAATAAATATTTATA+AGG - contig380end:8604-8623 None:intergenic 10.0%
!!! ATAGTTTATTTGTTAAAAAA+TGG + contig380end:8668-8687 MsG0080048633.01.T01:intron 10.0%
!!! TAGTTTATTTGTTAAAAAAT+GGG + contig380end:8669-8688 MsG0080048633.01.T01:intron 10.0%
!! AAAAAAAATCAACAATCATT+TGG + contig380end:8362-8381 MsG0080048633.01.T01:intron 15.0%
!! AAAAAAATCAACAATCATTT+GGG + contig380end:8363-8382 MsG0080048633.01.T01:intron 15.0%
!! AATTAAATAAGATGATGATT+TGG - contig380end:8037-8056 None:intergenic 15.0%
!! ACAAATAAAAATGAAAACTT+TGG - contig380end:8723-8742 None:intergenic 15.0%
!! AATTAGTGTGATATTTGTAA+TGG + contig380end:9131-9150 MsG0080048633.01.T01:three_prime_UTR 20.0%
!! ATGTAACAAATAACTAGTAA+TGG + contig380end:9067-9086 MsG0080048633.01.T01:three_prime_UTR 20.0%
!! TATGTGATGAAATCAATAAA+TGG + contig380end:9247-9266 MsG0080048633.01.T01:three_prime_UTR 20.0%
!! TGTAACAAATAACTAGTAAT+GGG + contig380end:9068-9087 MsG0080048633.01.T01:three_prime_UTR 20.0%
!!! TGTTGATTTTTTTTGTCATA+AGG - contig380end:8356-8375 None:intergenic 20.0%
! AAAAACACTTATAAGCTCTT+TGG + contig380end:8829-8848 MsG0080048633.01.T01:CDS 25.0%
! AACCACAGTTATTCTTTATA+TGG - contig380end:8579-8598 None:intergenic 25.0%
! AGATTATGAGATCTCAAAAA+TGG + contig380end:8458-8477 MsG0080048633.01.T01:intron 25.0%
! ATAAGAAAGAAACAAGTACA+TGG + contig380end:8142-8161 MsG0080048633.01.T01:intron 25.0%
! ATATTGTTGCATATGCATAT+TGG + contig380end:9155-9174 MsG0080048633.01.T01:three_prime_UTR 25.0%
! TGAATATGAAAAGATCAATG+AGG + contig380end:8942-8961 MsG0080048633.01.T01:CDS 25.0%
! TGGGAAGAAAAAAAAAATCA+GGG - contig380end:8225-8244 None:intergenic 25.0%
! TTGGGAAGAAAAAAAAAATC+AGG - contig380end:8226-8245 None:intergenic 25.0%
!! CTATCTTGAAAATGGTTTTT+GGG - contig380end:8244-8263 None:intergenic 25.0%
!! TCTATCTTGAAAATGGTTTT+TGG - contig380end:8245-8264 None:intergenic 25.0%
AACATAGTCAAAAGTAGAAG+AGG - contig380end:8287-8306 None:intergenic 30.0%
AGATATTGATGAGCACTATA+AGG + contig380end:8855-8874 MsG0080048633.01.T01:CDS 30.0%
AGCCATATAAAGAATAACTG+TGG + contig380end:8574-8593 MsG0080048633.01.T01:intron 30.0%
ATCCTTGAAGTCATTGATAA+GGG + contig380end:8889-8908 MsG0080048633.01.T01:CDS 30.0%
CATCATACAAAACTTCATGA+AGG + contig380end:7924-7943 MsG0080048633.01.T01:CDS 30.0%
CTTCTATGTGATGTTTGTAA+TGG + contig380end:9178-9197 MsG0080048633.01.T01:three_prime_UTR 30.0%
GGGAAGAAAAAAAAAATCAG+GGG - contig380end:8224-8243 None:intergenic 30.0%
TACATTCTACAAACCAAACA+TGG + contig380end:8195-8214 MsG0080048633.01.T01:intron 30.0%
TATGATGAACTCTTTCACAA+AGG - contig380end:7911-7930 None:intergenic 30.0%
TTATAGAGCTATCTTTGCTT+TGG - contig380end:9037-9056 None:intergenic 30.0%
! TGAAGCATCTATCTTGAAAA+TGG - contig380end:8252-8271 None:intergenic 30.0%
! TTTTTGTCATAAGGTTCTCT+GGG - contig380end:8347-8366 None:intergenic 30.0%
!! CTACTTTTGACTATGTTGAA+CGG + contig380end:8290-8309 MsG0080048633.01.T01:intron 30.0%
!! TACTTTTGACTATGTTGAAC+GGG + contig380end:8291-8310 MsG0080048633.01.T01:intron 30.0%
!! TAGTAATGGGTTATTGCATT+GGG + contig380end:9081-9100 MsG0080048633.01.T01:three_prime_UTR 30.0%
!!! TTTTTTGTCATAAGGTTCTC+TGG - contig380end:8348-8367 None:intergenic 30.0%
AACACTTATAAGCTCTTTGG+TGG + contig380end:8832-8851 MsG0080048633.01.T01:CDS 35.0%
AACTTCATGAAGGTGAAGAT+TGG + contig380end:7934-7953 MsG0080048633.01.T01:CDS 35.0%
AAGTATTCCATGTAGCCATT+TGG - contig380end:8979-8998 None:intergenic 35.0%
AATGGCTACATGGAATACTT+TGG + contig380end:8979-8998 MsG0080048633.01.T01:CDS 35.0%
AGCACCATTTCTTAGAAGAA+AGG - contig380end:7798-7817 None:intergenic 35.0%
ATCATAATTCACAGATGGCA+AGG + contig380end:8759-8778 MsG0080048633.01.T01:intron 35.0%
ATCTGTGAATTATGATGGCA+CGG - contig380end:8755-8774 None:intergenic 35.0%
CATCCTTGAAGTCATTGATA+AGG + contig380end:8888-8907 MsG0080048633.01.T01:CDS 35.0%
GAACAGATTGTAGAACTTGT+CGG - contig380end:7862-7881 None:intergenic 35.0%
GCACCATTTCTTAGAAGAAA+GGG - contig380end:7797-7816 None:intergenic 35.0%
TTGCCATCTGTGAATTATGA+TGG - contig380end:8760-8779 None:intergenic 35.0%
! CTTCCCTTTCTTCTAAGAAA+TGG + contig380end:7791-7810 MsG0080048633.01.T01:exon 35.0%
! TTTTGTCATAAGGTTCTCTG+GGG - contig380end:8346-8365 None:intergenic 35.0%
!! CTAGTAATGGGTTATTGCAT+TGG + contig380end:9080-9099 MsG0080048633.01.T01:three_prime_UTR 35.0%
!! TGATGATTTGGTTATCGATC+AGG - contig380end:8025-8044 None:intergenic 35.0%
AAACACTGGACTTACGTCAT+AGG + contig380end:7975-7994 MsG0080048633.01.T01:CDS 40.0%
AAATCAGGGGTAACCATGTT+TGG - contig380end:8211-8230 None:intergenic 40.0%
AGTGTGATGTCTGTACATGA+CGG + contig380end:9205-9224 MsG0080048633.01.T01:three_prime_UTR 40.0%
ATTCCATGTAGCCATTTGGA+GGG - contig380end:8975-8994 None:intergenic 40.0%
CACCCTTATCAATGACTTCA+AGG - contig380end:8894-8913 None:intergenic 40.0%
GAAGTCATTGATAAGGGTGA+TGG + contig380end:8895-8914 MsG0080048633.01.T01:CDS 40.0%
GTGCCATCATAATTCACAGA+TGG + contig380end:8754-8773 MsG0080048633.01.T01:intron 40.0%
TATTCCATGTAGCCATTTGG+AGG - contig380end:8976-8995 None:intergenic 40.0%
TCTGTGAATTATGATGGCAC+GGG - contig380end:8754-8773 None:intergenic 40.0%
! AGATTGGCATCACACTGATT+CGG + contig380end:7950-7969 MsG0080048633.01.T01:CDS 40.0%
! ATAAGGTTCTCTGGGGAATA+TGG - contig380end:8339-8358 None:intergenic 40.0%
! TTCTAAGAAATGGTGCTCTC+TGG + contig380end:7801-7820 MsG0080048633.01.T01:exon 40.0%
!! AATGGGTTATTGCATTGGGT+TGG + contig380end:9085-9104 MsG0080048633.01.T01:three_prime_UTR 40.0%
!! ACACTGATTCGGTTAAACAC+TGG + contig380end:7961-7980 MsG0080048633.01.T01:CDS 40.0%
AGATTGTAGAACTTGTCGGC+GGG - contig380end:7858-7877 None:intergenic 45.0%
CAGATTGTAGAACTTGTCGG+CGG - contig380end:7859-7878 None:intergenic 45.0%
GAGGATATTGACCCTCCAAA+TGG + contig380end:8961-8980 MsG0080048633.01.T01:CDS 45.0%
! GGTAAGCTCAGTACTGAAGT+TGG + contig380end:7822-7841 MsG0080048633.01.T01:CDS 45.0%
!!! ATATTTTTGTATAAAAAAAA+AGG - contig380end:8624-8643 None:intergenic 5.0%
!!! TTTTATACAAAAATATTTAT+TGG + contig380end:8628-8647 MsG0080048633.01.T01:intron 5.0%
ATGGCACTGCTGCTGCTAAA+TGG + contig380end:8914-8933 MsG0080048633.01.T01:CDS 50.0%
TGACCCTCCAAATGGCTACA+TGG + contig380end:8969-8988 MsG0080048633.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
contig380end gene 7757 9282 7757 ID=MsG0080048633.01;Name=MsG0080048633.01
contig380end mRNA 7757 9282 7757 ID=MsG0080048633.01.T01;Parent=MsG0080048633.01;Name=MsG0080048633.01.T01;_AED=0.26;_eAED=0.26;_QI=53|1|1|1|1|1|2|226|156
contig380end exon 8773 9282 8773 ID=MsG0080048633.01.T01:exon:3934;Parent=MsG0080048633.01.T01
contig380end exon 7757 7996 7757 ID=MsG0080048633.01.T01:exon:3933;Parent=MsG0080048633.01.T01
contig380end five_prime_UTR 7757 7809 7757 ID=MsG0080048633.01.T01:five_prime_utr;Parent=MsG0080048633.01.T01
contig380end CDS 7810 7996 7810 ID=MsG0080048633.01.T01:cds;Parent=MsG0080048633.01.T01
contig380end CDS 8773 9056 8773 ID=MsG0080048633.01.T01:cds;Parent=MsG0080048633.01.T01
contig380end three_prime_UTR 9057 9282 9057 ID=MsG0080048633.01.T01:three_prime_utr;Parent=MsG0080048633.01.T01
Gene Sequence

>MsG0080048633.01.T01

ATGGTGCTCTCTGGTAAGCTCAGTACTGAAGTTGGAATCAAAACACCCGCCGACAAGTTCTACAATCTGTTCATAACAGAACTTCATGAAGTGCAAAACCTTTGTGAAAGAGTTCATCATACAAAACTTCATGAAGGTGAAGATTGGCATCACACTGATTCGGTTAAACACTGGACTTACGTCATAGATGGCAAGGTACATACATGTCACGAGAGAGTTGAAGAAGTTGATGAACTGAATAAAAAAAACACTTATAAGCTCTTTGGTGGAGATATTGATGAGCACTATAAGGTCTTTAAGCTCATCCTTGAAGTCATTGATAAGGGTGATGGCACTGCTGCTGCTAAATGGACAGTTGAATATGAAAAGATCAATGAGGATATTGACCCTCCAAATGGCTACATGGAATACTTTGGCAAAATCACTAGAGATGTTGATGCTCATCTTGCCAAAGCAAAGATAGCTCTATAA

Protein sequence

>MsG0080048633.01.T01

MVLSGKLSTEVGIKTPADKFYNLFITELHEVQNLCERVHHTKLHEGEDWHHTDSVKHWTYVIDGKVHTCHERVEEVDELNKKNTYKLFGGDIDEHYKVFKLILEVIDKGDGTAAAKWTVEYEKINEDIDPPNGYMEYFGKITRDVDAHLAKAKIAL*