AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001108.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001108.01.T01 MTR_1g030820 94.118 153 9 0 1 153 1 153 6.86e-103 291
MsG0180001108.01.T01 MTR_1g030810 87.582 153 19 0 1 153 1 153 1.49e-97 278
MsG0180001108.01.T01 MTR_1g030840 58.710 155 59 2 1 150 1 155 2.53e-61 186
MsG0180001108.01.T01 MTR_8g045490 41.176 153 87 2 1 152 1 151 1.10e-38 129
MsG0180001108.01.T01 MTR_8g045570 40.523 153 88 2 1 152 1 151 1.66e-38 128
MsG0180001108.01.T01 MTR_8g045400 39.869 153 88 3 1 152 1 150 4.64e-37 125
MsG0180001108.01.T01 MTR_8g045695 39.216 153 89 3 1 152 1 150 7.01e-37 124
MsG0180001108.01.T01 MTR_8g045560 39.216 153 89 3 1 152 1 150 1.35e-36 124
MsG0180001108.01.T01 MTR_8g045735 38.562 153 90 3 1 152 1 150 2.21e-36 123
MsG0180001108.01.T01 MTR_8g045555 37.255 153 93 2 1 152 1 151 2.35e-36 123
MsG0180001108.01.T01 MTR_8g045520 38.312 154 92 2 1 153 1 152 8.90e-36 121
MsG0180001108.01.T01 MTR_8g045300 39.610 154 90 2 1 153 1 152 1.08e-34 119
MsG0180001108.01.T01 MTR_8g045640 37.908 153 91 3 1 152 1 150 1.25e-34 118
MsG0180001108.01.T01 MTR_8g045665 37.255 153 92 3 1 152 1 150 1.38e-34 118
MsG0180001108.01.T01 MTR_8g012550 35.897 156 94 4 1 153 1 153 2.52e-28 102
MsG0180001108.01.T01 MTR_1g031640 37.013 154 87 5 1 152 1 146 3.52e-27 99.4
MsG0180001108.01.T01 MTR_5g035010 28.758 153 102 5 1 150 3 151 1.55e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001108.01.T01 AT1G70890 37.662 154 92 3 2 153 6 157 2.10e-32 113
MsG0180001108.01.T01 AT5G28010 38.961 154 90 3 2 153 14 165 1.05e-31 111
MsG0180001108.01.T01 AT5G28010 38.961 154 90 3 2 153 14 165 1.05e-31 111
MsG0180001108.01.T01 AT1G70840 35.065 154 96 3 2 153 19 170 5.48e-31 110
MsG0180001108.01.T01 AT1G70830 37.013 154 93 3 2 153 21 172 1.65e-30 108
MsG0180001108.01.T01 AT1G70830 37.013 154 93 3 2 153 21 172 3.13e-30 108
MsG0180001108.01.T01 AT1G70830 37.013 154 93 3 2 153 21 172 1.76e-29 108
MsG0180001108.01.T01 AT1G70830 37.013 154 93 3 2 153 21 172 6.60e-29 108
MsG0180001108.01.T01 AT1G70830 36.364 154 94 3 2 153 183 334 1.52e-27 105
MsG0180001108.01.T01 AT1G70830 36.364 154 94 3 2 153 136 287 4.53e-28 105
MsG0180001108.01.T01 AT1G70830 36.458 96 58 2 59 153 32 125 4.22e-14 68.2
MsG0180001108.01.T01 AT1G70850 34.211 152 96 3 2 151 8 157 5.98e-27 101
MsG0180001108.01.T01 AT5G28000 33.766 154 98 3 2 153 12 163 2.63e-26 97.8
MsG0180001108.01.T01 AT1G70850 34.211 152 96 3 2 151 8 157 2.79e-26 101
MsG0180001108.01.T01 AT1G70850 35.664 143 88 3 13 153 175 315 2.28e-25 99.0
MsG0180001108.01.T01 AT1G70850 34.211 152 96 3 2 151 8 157 2.79e-26 101
MsG0180001108.01.T01 AT1G70850 35.664 143 88 3 13 153 175 315 2.28e-25 99.0
MsG0180001108.01.T01 AT1G70880 32.468 154 100 3 2 153 7 158 2.30e-25 95.1
MsG0180001108.01.T01 AT1G23130 27.451 153 108 2 2 153 9 159 7.09e-25 94.0
MsG0180001108.01.T01 AT1G35260 31.293 147 96 3 7 151 6 149 1.50e-23 90.5
MsG0180001108.01.T01 AT1G14930 35.294 153 95 3 1 152 1 150 3.71e-23 89.4
MsG0180001108.01.T01 AT1G70870 36.842 152 82 5 1 152 1 138 6.63e-23 88.2
MsG0180001108.01.T01 AT1G23120 31.373 153 99 3 1 153 1 147 2.92e-21 84.3
MsG0180001108.01.T01 AT1G23120 31.373 153 99 3 1 153 51 197 8.64e-21 84.3
MsG0180001108.01.T01 AT1G14950 30.065 153 103 3 1 152 1 150 1.33e-18 77.8
MsG0180001108.01.T01 AT4G14060 30.065 153 103 3 1 152 1 150 3.00e-18 76.6
MsG0180001108.01.T01 AT1G35310 30.282 142 95 3 13 153 12 150 3.10e-18 76.6
MsG0180001108.01.T01 AT1G24020 36.420 162 84 7 1 153 1 152 6.38e-18 75.9
MsG0180001108.01.T01 AT1G24020 36.420 162 84 7 1 153 1 152 6.38e-18 75.9
MsG0180001108.01.T01 AT2G01520 29.412 153 104 3 1 152 1 150 2.68e-17 74.3
MsG0180001108.01.T01 AT1G14940 29.605 152 103 3 1 151 1 149 1.12e-16 72.8
MsG0180001108.01.T01 AT1G30990 27.632 152 105 3 1 151 1 148 8.20e-16 70.5
MsG0180001108.01.T01 AT2G01530 29.412 153 104 3 1 152 1 150 4.78e-15 68.2
MsG0180001108.01.T01 AT1G14960 28.105 153 106 3 1 152 1 150 7.23e-15 67.8
MsG0180001108.01.T01 AT3G26460 28.105 153 107 2 1 152 1 151 2.66e-13 63.9
MsG0180001108.01.T01 AT3G26450 28.571 154 105 4 1 152 1 151 4.61e-13 63.2
MsG0180001108.01.T01 AT4G23670 29.412 153 104 3 1 152 1 150 7.82e-13 62.4

Find 31 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGACTTCTGTGGTGATATT+AGG 0.304651 1:-16085569 None:intergenic
TGAGAGTTACAAGAGTTTAA+AGG 0.313337 1:+16086441 MsG0180001108.01.T01:CDS
ATGGTGGTGGGATTGTTAAA+TGG 0.324878 1:+16086491 MsG0180001108.01.T01:CDS
GAAGGTGACTGGGAAAATAT+TGG 0.418929 1:+16085608 MsG0180001108.01.T01:CDS
ATACTTGGACCTTGCTGTAA+AGG 0.426075 1:+16086567 MsG0180001108.01.T01:CDS
AAGCTAAAGGAGGAAATTAC+AGG 0.430297 1:+16086529 MsG0180001108.01.T01:CDS
CGTATCTCCTGATTCATACT+TGG 0.435164 1:+16086552 MsG0180001108.01.T01:CDS
GCAAGGTCCAAGTATGAATC+AGG 0.441032 1:-16086559 None:intergenic
TGGTGGTGGGATTGTTAAAT+GGG 0.470252 1:+16086492 MsG0180001108.01.T01:CDS
AGAAGAGACATGGCTTTAAG+TGG 0.509760 1:+16085467 None:intergenic
CATGGCTTTAAGTGGGAAAG+TGG 0.512659 1:+16085475 None:intergenic
GTAGAGTGCATGAAGGTGAC+TGG 0.523294 1:+16085597 MsG0180001108.01.T01:CDS
ATTCATAAAGAACATGGTGG+TGG 0.537340 1:+16086478 MsG0180001108.01.T01:CDS
ATATTGGTTCTATCAAGCAC+TGG 0.538736 1:+16085624 MsG0180001108.01.T01:CDS
CTTCATGCACTCTACCTCCA+TGG 0.564742 1:-16085589 None:intergenic
TTCATAAAGAACATGGTGGT+GGG 0.570285 1:+16086479 MsG0180001108.01.T01:CDS
GAAGAGACATGGCTTTAAGT+GGG 0.591992 1:+16085468 None:intergenic
TATTGGTTCTATCAAGCACT+GGG 0.594586 1:+16085625 MsG0180001108.01.T01:CDS
AATATCACCACAGAAGTCCA+TGG 0.603582 1:+16085572 MsG0180001108.01.T01:CDS
TAGAGTGCATGAAGGTGACT+GGG 0.605256 1:+16085598 MsG0180001108.01.T01:CDS
CAAGTGATTCATAAAGAACA+TGG 0.605539 1:+16086472 MsG0180001108.01.T01:CDS
TATTGATTCTCATCTTGTCA+AGG 0.605627 1:+16086600 MsG0180001108.01.T01:CDS
TCTACCTCCATGGACTTCTG+TGG 0.609185 1:-16085579 None:intergenic
ATACGAATATGAGAAGCTAA+AGG 0.610623 1:+16086516 MsG0180001108.01.T01:CDS
AAGCACTGGGAATTTACAGT+AGG 0.622307 1:+16085638 MsG0180001108.01.T01:CDS
CGAATATGAGAAGCTAAAGG+AGG 0.640547 1:+16086519 MsG0180001108.01.T01:CDS
CATGGAGGTAGAGTGCATGA+AGG 0.659264 1:+16085590 MsG0180001108.01.T01:CDS
GAAGTAGAGATTCAAGCACC+TGG 0.664321 1:+16085503 MsG0180001108.01.T01:CDS
ATATTATAGAACTTAGCACC+AGG 0.680852 1:-16085521 None:intergenic
GTGATTCATAAAGAACATGG+TGG 0.735641 1:+16086475 MsG0180001108.01.T01:CDS
ATCACCACAGAAGTCCATGG+AGG 0.764074 1:+16085575 MsG0180001108.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGCTTAAATAATATTTT+AGG - Chr1:16085980-16085999 None:intergenic 10.0%
!!! AAAGCTTAAATAATATTTTA+GGG - Chr1:16085979-16085998 None:intergenic 10.0%
!!! AAATAAAAGATTAAAAGTAT+AGG + Chr1:16085902-16085921 MsG0180001108.01.T01:intron 10.0%
!!! AATAAAAGATTAAAAGTATA+GGG + Chr1:16085903-16085922 MsG0180001108.01.T01:intron 10.0%
!!! TAAAATATTATTTAAGCTTT+TGG + Chr1:16085979-16085998 MsG0180001108.01.T01:intron 10.0%
!! AATAAGCAAATGAAAAAAAA+AGG - Chr1:16086204-16086223 None:intergenic 15.0%
!! ATTTGCTTATTAAAAAAACA+AGG + Chr1:16086213-16086232 MsG0180001108.01.T01:intron 15.0%
!!! AAGCTTAAATAATATTTTAG+GGG - Chr1:16085978-16085997 None:intergenic 15.0%
!!! ATTTCATGAATGAATTTTTT+TGG - Chr1:16086010-16086029 None:intergenic 15.0%
!!! TAATGAAATTTTCCATAAAT+TGG + Chr1:16086104-16086123 MsG0180001108.01.T01:intron 15.0%
!! CAAACATTGATATATTTAGA+GGG - Chr1:16085946-16085965 None:intergenic 20.0%
!! TAGTAAAACTATGAAACATA+AGG - Chr1:16085674-16085693 None:intergenic 20.0%
!! TATAGGGTTAAATAAGTAAA+TGG + Chr1:16085919-16085938 MsG0180001108.01.T01:intron 20.0%
!! TCAAACATTGATATATTTAG+AGG - Chr1:16085947-16085966 None:intergenic 20.0%
!! TGTGTTTATAAATGACAATT+TGG - Chr1:16085847-16085866 None:intergenic 20.0%
!!! ACATAATTTTCTACCATATA+TGG - Chr1:16086037-16086056 None:intergenic 20.0%
!!! AGCTTAAATAATATTTTAGG+GGG - Chr1:16085977-16085996 None:intergenic 20.0%
!!! ATCATATATAGCATTTTTGA+TGG + Chr1:16086409-16086428 MsG0180001108.01.T01:CDS 20.0%
!!! CATAATTTTCTACCAATTTA+TGG - Chr1:16086119-16086138 None:intergenic 20.0%
!!! TAAATATGTTTTGTTTACGT+TGG + Chr1:16086256-16086275 MsG0180001108.01.T01:intron 20.0%
!!! TTATTTATTTTCCCTAATCA+TGG + Chr1:16086293-16086312 MsG0180001108.01.T01:intron 20.0%
! GAATTTACAGTAGGTAATTA+AGG + Chr1:16085647-16085666 MsG0180001108.01.T01:intron 25.0%
! TCATGAAATTAGTCCATATA+TGG + Chr1:16086021-16086040 MsG0180001108.01.T01:intron 25.0%
!! ACTATTTTATTGCTCATGAA+AGG + Chr1:16085690-16085709 MsG0180001108.01.T01:intron 25.0%
!!! ATGAATGAATTTTTTTGGAG+GGG - Chr1:16086005-16086024 None:intergenic 25.0%
!!! CATGAATGAATTTTTTTGGA+GGG - Chr1:16086006-16086025 None:intergenic 25.0%
!!! TCATGAATGAATTTTTTTGG+AGG - Chr1:16086007-16086026 None:intergenic 25.0%
AAAATCAGAGTTCCATGATT+AGG - Chr1:16086308-16086327 None:intergenic 30.0%
AAATCAGAGTTCCATGATTA+GGG - Chr1:16086307-16086326 None:intergenic 30.0%
ATACGAATATGAGAAGCTAA+AGG + Chr1:16086516-16086535 MsG0180001108.01.T01:CDS 30.0%
ATATTATAGAACTTAGCACC+AGG - Chr1:16085524-16085543 None:intergenic 30.0%
CAAGTGATTCATAAAGAACA+TGG + Chr1:16086472-16086491 MsG0180001108.01.T01:CDS 30.0%
TGAGAGTTACAAGAGTTTAA+AGG + Chr1:16086441-16086460 MsG0180001108.01.T01:CDS 30.0%
! TAACATCAACAAAAGAGTGT+TGG - Chr1:16085759-16085778 None:intergenic 30.0%
!! TATTGATTCTCATCTTGTCA+AGG + Chr1:16086600-16086619 MsG0180001108.01.T01:CDS 30.0%
!! TTTGTTTTCTTCCTTCTGTA+GGG - Chr1:16086345-16086364 None:intergenic 30.0%
AAGCTAAAGGAGGAAATTAC+AGG + Chr1:16086529-16086548 MsG0180001108.01.T01:CDS 35.0%
ATTCATAAAGAACATGGTGG+TGG + Chr1:16086478-16086497 MsG0180001108.01.T01:CDS 35.0%
GTGATTCATAAAGAACATGG+TGG + Chr1:16086475-16086494 MsG0180001108.01.T01:CDS 35.0%
TTCATAAAGAACATGGTGGT+GGG + Chr1:16086479-16086498 MsG0180001108.01.T01:CDS 35.0%
! AGCATTTTTGATGGAGAAGT+GGG + Chr1:16086418-16086437 MsG0180001108.01.T01:CDS 35.0%
! TAGCATTTTTGATGGAGAAG+TGG + Chr1:16086417-16086436 MsG0180001108.01.T01:CDS 35.0%
!! ATATTGGTTCTATCAAGCAC+TGG + Chr1:16085624-16085643 MsG0180001108.01.T01:CDS 35.0%
!! ATCAACAAAAGAGTGTTGGA+AGG - Chr1:16085755-16085774 None:intergenic 35.0%
!! TATTGGTTCTATCAAGCACT+GGG + Chr1:16085625-16085644 MsG0180001108.01.T01:CDS 35.0%
!! TCAACAAAAGAGTGTTGGAA+GGG - Chr1:16085754-16085773 None:intergenic 35.0%
!!! CTTTGTTTTCTTCCTTCTGT+AGG - Chr1:16086346-16086365 None:intergenic 35.0%
AATATCACCACAGAAGTCCA+TGG + Chr1:16085572-16085591 MsG0180001108.01.T01:CDS 40.0%
CGAATATGAGAAGCTAAAGG+AGG + Chr1:16086519-16086538 MsG0180001108.01.T01:CDS 40.0%
CGTATCTCCTGATTCATACT+TGG + Chr1:16086552-16086571 MsG0180001108.01.T01:CDS 40.0%
GAAGGTGACTGGGAAAATAT+TGG + Chr1:16085608-16085627 MsG0180001108.01.T01:CDS 40.0%
TACTTGAGAAACCCTACAGA+AGG + Chr1:16086331-16086350 MsG0180001108.01.T01:intron 40.0%
TGGACTTCTGTGGTGATATT+AGG - Chr1:16085572-16085591 None:intergenic 40.0%
! ATACTTGGACCTTGCTGTAA+AGG + Chr1:16086567-16086586 MsG0180001108.01.T01:CDS 40.0%
! CTTTTGTGACCTTTACAGCA+AGG - Chr1:16086579-16086598 None:intergenic 40.0%
!! AAGCACTGGGAATTTACAGT+AGG + Chr1:16085638-16085657 MsG0180001108.01.T01:CDS 40.0%
!! ATGGTGGTGGGATTGTTAAA+TGG + Chr1:16086491-16086510 MsG0180001108.01.T01:CDS 40.0%
!! TGGTGGTGGGATTGTTAAAT+GGG + Chr1:16086492-16086511 MsG0180001108.01.T01:CDS 40.0%
GAAGTAGAGATTCAAGCACC+TGG + Chr1:16085503-16085522 MsG0180001108.01.T01:CDS 45.0%
GCAAGGTCCAAGTATGAATC+AGG - Chr1:16086562-16086581 None:intergenic 45.0%
TAGAGTGCATGAAGGTGACT+GGG + Chr1:16085598-16085617 MsG0180001108.01.T01:CDS 45.0%
!!! TTAAAAAATTCTTTTTTTTT+TGG - Chr1:16086152-16086171 None:intergenic 5.0%
ATCACCACAGAAGTCCATGG+AGG + Chr1:16085575-16085594 MsG0180001108.01.T01:CDS 50.0%
CATGGAGGTAGAGTGCATGA+AGG + Chr1:16085590-16085609 MsG0180001108.01.T01:CDS 50.0%
CTTCATGCACTCTACCTCCA+TGG - Chr1:16085592-16085611 None:intergenic 50.0%
GTAGAGTGCATGAAGGTGAC+TGG + Chr1:16085597-16085616 MsG0180001108.01.T01:CDS 50.0%
TCTACCTCCATGGACTTCTG+TGG - Chr1:16085582-16085601 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 16085476 16086627 16085476 ID=MsG0180001108.01;Name=MsG0180001108.01
Chr1 mRNA 16085476 16086627 16085476 ID=MsG0180001108.01.T01;Parent=MsG0180001108.01;Name=MsG0180001108.01.T01;_AED=0.41;_eAED=0.41;_QI=0|0|0|1|1|1|2|0|153
Chr1 exon 16085476 16085659 16085476 ID=MsG0180001108.01.T01:exon:31196;Parent=MsG0180001108.01.T01
Chr1 exon 16086350 16086627 16086350 ID=MsG0180001108.01.T01:exon:31197;Parent=MsG0180001108.01.T01
Chr1 CDS 16085476 16085659 16085476 ID=MsG0180001108.01.T01:cds;Parent=MsG0180001108.01.T01
Chr1 CDS 16086350 16086627 16086350 ID=MsG0180001108.01.T01:cds;Parent=MsG0180001108.01.T01
Gene Sequence

>MsG0180001108.01.T01

ATGGCTTTAAGTGGGAAAGTGGAAGCTGAAGTAGAGATTCAAGCACCTGGTGCTAAGTTCTATAATATTTTCAGAAAGCAACTAGAACATCTTCCTAATATCACCACAGAAGTCCATGGAGGTAGAGTGCATGAAGGTGACTGGGAAAATATTGGTTCTATCAAGCACTGGGAATTTACAGTAGAAGGAAGAAAACAAAGTGCTAAAGCAAAAATTGAGACTATAGATGATGACAATAAGATAATCATATATAGCATTTTTGATGGAGAAGTGGGTGAGAGTTACAAGAGTTTAAAGGCAATATTACAAGTGATTCATAAAGAACATGGTGGTGGGATTGTTAAATGGGCATACGAATATGAGAAGCTAAAGGAGGAAATTACAGGCGTATCTCCTGATTCATACTTGGACCTTGCTGTAAAGGTCACAAAAGATATTGATTCTCATCTTGTCAAGGAATAG

Protein sequence

>MsG0180001108.01.T01

MALSGKVEAEVEIQAPGAKFYNIFRKQLEHLPNITTEVHGGRVHEGDWENIGSIKHWEFTVEGRKQSAKAKIETIDDDNKIIIYSIFDGEVGESYKSLKAILQVIHKEHGGGIVKWAYEYEKLKEEITGVSPDSYLDLAVKVTKDIDSHLVKE*