AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002271.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002271.01.T01 MTR_7g065630 91.667 120 10 0 31 150 120 239 4.11e-75 224
MsG0180002271.01.T01 MTR_7g065680 76.667 120 28 0 31 150 102 221 9.88e-54 169
MsG0180002271.01.T01 MTR_7g065710 72.115 104 29 0 36 139 104 207 1.90e-49 158
MsG0180002271.01.T01 MTR_7g065700 71.560 109 31 0 31 139 102 210 1.97e-44 145
MsG0180002271.01.T01 MTR_7g065590 58.197 122 47 1 31 148 102 223 3.21e-44 145
MsG0180002271.01.T01 MTR_7g065600 59.350 123 46 1 30 148 101 223 1.14e-42 141
MsG0180002271.01.T01 MTR_7g065660 57.258 124 49 1 31 150 102 225 1.97e-42 140
MsG0180002271.01.T01 MTR_7g065230 61.345 119 45 1 30 148 101 218 2.12e-41 137
MsG0180002271.01.T01 MTR_7g065290 56.667 120 48 1 33 148 104 223 1.04e-39 133
MsG0180002271.01.T01 MTR_7g065740 54.400 125 52 2 31 150 102 226 1.95e-39 133
MsG0180002271.01.T01 MTR_7g065720 58.678 121 45 2 33 148 104 224 3.34e-37 127
MsG0180002271.01.T01 MTR_7g065750 62.264 106 36 1 49 150 121 226 6.01e-37 126
MsG0180002271.01.T01 MTR_4g019790 53.279 122 49 2 33 150 108 225 1.84e-36 125
MsG0180002271.01.T01 MTR_7g065265 53.333 120 52 1 33 148 104 223 1.76e-34 120
MsG0180002271.01.T01 MTR_7g065260 64.835 91 27 2 63 148 141 231 7.68e-34 119
MsG0180002271.01.T01 MTR_7g065270 50.000 120 56 1 33 148 104 223 6.06e-33 116
MsG0180002271.01.T01 MTR_4g019780 52.137 117 50 3 31 142 103 218 2.85e-32 114
MsG0180002271.01.T01 MTR_7g065640 50.833 120 45 1 31 150 102 207 7.20e-32 113
MsG0180002271.01.T01 MTR_6g080440 34.921 126 73 4 31 148 98 222 2.62e-16 73.2
MsG0180002271.01.T01 MTR_8g087410 30.172 116 75 3 40 150 110 224 5.75e-15 69.3
MsG0180002271.01.T01 MTR_1g090070 35.849 106 61 2 51 149 124 229 2.06e-14 68.2
MsG0180002271.01.T01 MTR_5g040430 33.019 106 61 2 45 140 116 221 6.49e-12 61.2
MsG0180002271.01.T01 MTR_1g090150 33.333 114 69 3 43 149 108 221 1.07e-11 60.8
MsG0180002271.01.T01 MTR_1g090060 30.769 104 65 2 51 147 117 220 1.95e-11 60.1
MsG0180002271.01.T01 MTR_1g090100 32.381 105 64 2 51 148 116 220 4.76e-11 58.9
MsG0180002271.01.T01 MTR_8g087425 32.000 75 47 1 75 145 144 218 5.17e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002271.01.T01 AT3G09270 44.118 102 52 2 52 148 123 224 5.94e-18 77.4
MsG0180002271.01.T01 AT2G29420 33.036 112 70 2 43 149 115 226 9.67e-15 68.9
MsG0180002271.01.T01 AT2G29450 28.226 124 83 3 31 148 101 224 4.29e-11 58.9

Find 24 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCATAATTTCCTTGACTAT+TGG 0.252367 1:-36054284 None:intergenic
ACTGCAATTCTGCTACTTCT+TGG 0.255928 1:-36054202 None:intergenic
TCTGCTACTTCTTGGAATAA+AGG 0.260381 1:-36054194 None:intergenic
GAATTGAAGGGAAAATTCTT+TGG 0.311076 1:+36054128 MsG0180002271.01.T01:CDS
CTGCTACTTCTTGGAATAAA+GGG 0.317178 1:-36054193 None:intergenic
TTACTCCATTTGTATAGCTT+TGG 0.321457 1:-36054245 None:intergenic
TTTCTCGCGTTCTTTCTTAT+CGG 0.326801 1:-36054063 None:intergenic
TTCTTTGGAGGAGAAGAGAT+TGG 0.382580 1:+36054143 MsG0180002271.01.T01:CDS
TTCTCGCGTTCTTTCTTATC+GGG 0.402746 1:-36054062 None:intergenic
AGCTAGCGACGAACTATTGA+AGG 0.446209 1:+36054085 MsG0180002271.01.T01:CDS
ATTTCTTGAGAATGAATTGA+AGG 0.482186 1:+36054115 MsG0180002271.01.T01:CDS
GTGGAGAAAGAGATGGGATG+TGG 0.484017 1:+36051014 MsG0180002271.01.T01:CDS
TTGAAGGGAAAATTCTTTGG+AGG 0.490147 1:+36054131 MsG0180002271.01.T01:CDS
ATGAAAGTGGAGAAAGAGAT+GGG 0.494887 1:+36051008 MsG0180002271.01.T01:CDS
AATGAAAGTGGAGAAAGAGA+TGG 0.514963 1:+36051007 None:intergenic
AATTTCCAAAGCTATACAAA+TGG 0.517213 1:+36054240 MsG0180002271.01.T01:CDS
TTTCTTGAGAATGAATTGAA+GGG 0.518621 1:+36054116 MsG0180002271.01.T01:CDS
TAATTTCCTTGACTATTGGA+TGG 0.528427 1:-36054280 None:intergenic
TAAAGTGCAAGAAATGAAAG+TGG 0.536655 1:+36050995 None:intergenic
TGTATGAAACAATGAACAAA+TGG 0.550172 1:+36051048 MsG0180002271.01.T01:CDS
ATCAGTGTGTGGCTGCTGCA+TGG 0.567781 1:+36054024 MsG0180002271.01.T01:intron
TGTGGCTGCTGCATGGATGT+CGG 0.602873 1:+36054031 MsG0180002271.01.T01:CDS
TAACAACCATCCAATAGTCA+AGG 0.609813 1:+36054274 MsG0180002271.01.T01:CDS
AGAATTGCAGTTATTCACAA+AGG 0.625696 1:+36054214 MsG0180002271.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTTCAGAATTTTTAA+AGG + Chr1:36051760-36051779 MsG0180002271.01.T01:intron 10.0%
!!! TTGATTTTTTTTATGATTTT+TGG + Chr1:36052868-36052887 MsG0180002271.01.T01:intron 10.0%
!! AAAAAAAAGTAAAAATTCGT+TGG + Chr1:36051866-36051885 MsG0180002271.01.T01:intron 15.0%
!! AAAAAAAGTAAAAATTCGTT+GGG + Chr1:36051867-36051886 MsG0180002271.01.T01:intron 15.0%
!! AAAATTGAATTGAAATTCTT+TGG - Chr1:36051233-36051252 None:intergenic 15.0%
!! ATTTCAAAAAAATATCAGTT+AGG - Chr1:36053564-36053583 None:intergenic 15.0%
!!! AAAAGTACAAAGAAAATTAT+AGG + Chr1:36051737-36051756 MsG0180002271.01.T01:intron 15.0%
!!! AAGCATTTAAAACAAAAATT+GGG + Chr1:36051284-36051303 MsG0180002271.01.T01:intron 15.0%
!!! ACAATTTTTAGAATTTAAAC+AGG + Chr1:36053324-36053343 MsG0180002271.01.T01:intron 15.0%
!!! AGAATTAAACAAGTATTTTT+AGG + Chr1:36053768-36053787 MsG0180002271.01.T01:intron 15.0%
!!! ATTTTAAAATGGATTTTAAC+AGG + Chr1:36053257-36053276 MsG0180002271.01.T01:intron 15.0%
!!! CAATTTTTAGAATTTAAACA+GGG + Chr1:36053325-36053344 MsG0180002271.01.T01:intron 15.0%
!! ATAAGTTAAAAAACACATTC+AGG + Chr1:36051945-36051964 MsG0180002271.01.T01:intron 20.0%
!! ATTTATTCACAAACAAAACT+TGG + Chr1:36051320-36051339 MsG0180002271.01.T01:intron 20.0%
!! ATTTGCAAAATTCACATAAA+AGG - Chr1:36053674-36053693 None:intergenic 20.0%
!! CTTCAAATGCAATTTAAAAA+TGG + Chr1:36053403-36053422 MsG0180002271.01.T01:intron 20.0%
!! TATAATCCTTATACTTCAAA+GGG + Chr1:36051204-36051223 MsG0180002271.01.T01:intron 20.0%
!! TTTGCAAAATTCACATAAAA+GGG - Chr1:36053673-36053692 None:intergenic 20.0%
!!! ACTCAGATGAAATTTTAAAA+TGG + Chr1:36053246-36053265 MsG0180002271.01.T01:intron 20.0%
!!! CAAGCATTTAAAACAAAAAT+TGG + Chr1:36051283-36051302 MsG0180002271.01.T01:intron 20.0%
!!! CAATTTTTGTTTTAAATGCT+TGG - Chr1:36051285-36051304 None:intergenic 20.0%
!!! TATTGTTTTGGTAAATTTTG+AGG - Chr1:36052185-36052204 None:intergenic 20.0%
!!! TATTTTTAGGTCCAAAATTA+GGG + Chr1:36053781-36053800 MsG0180002271.01.T01:intron 20.0%
!!! TTTAAAAATGGATTTTGACA+TGG + Chr1:36053415-36053434 MsG0180002271.01.T01:intron 20.0%
! AATTTCCAAAGCTATACAAA+TGG + Chr1:36054240-36054259 MsG0180002271.01.T01:CDS 25.0%
! AGCAAAAACAAATGTGAAAT+AGG + Chr1:36051142-36051161 MsG0180002271.01.T01:intron 25.0%
! ATTTCTTGAGAATGAATTGA+AGG + Chr1:36054115-36054134 MsG0180002271.01.T01:CDS 25.0%
! CTATAATCCTTATACTTCAA+AGG + Chr1:36051203-36051222 MsG0180002271.01.T01:intron 25.0%
! TCACTCAAAACAAATGTAAA+AGG + Chr1:36051580-36051599 MsG0180002271.01.T01:intron 25.0%
! TCTTAAAGACCAATAACATT+TGG + Chr1:36053001-36053020 MsG0180002271.01.T01:intron 25.0%
! TCTTGGTTCATATCAAAAAA+CGG + Chr1:36052254-36052273 MsG0180002271.01.T01:intron 25.0%
! TGTATGAAACAATGAACAAA+TGG + Chr1:36051048-36051067 MsG0180002271.01.T01:CDS 25.0%
! TTACCACATCATCTATAAAT+GGG - Chr1:36052932-36052951 None:intergenic 25.0%
! TTATACATGAATACTCTTCA+TGG + Chr1:36053834-36053853 MsG0180002271.01.T01:intron 25.0%
! TTGAAACTTAACGTAAAATG+AGG + Chr1:36053132-36053151 MsG0180002271.01.T01:intron 25.0%
! TTTACCACATCATCTATAAA+TGG - Chr1:36052933-36052952 None:intergenic 25.0%
! TTTCTTGAGAATGAATTGAA+GGG + Chr1:36054116-36054135 MsG0180002271.01.T01:CDS 25.0%
!! ATTTTCACAAAGAGGATTAA+AGG + Chr1:36051248-36051267 MsG0180002271.01.T01:intron 25.0%
!! GTAAAAATTTTCACGTTTAG+AGG - Chr1:36052123-36052142 None:intergenic 25.0%
!! TCAATTCAATTTTCACAAAG+AGG + Chr1:36051240-36051259 MsG0180002271.01.T01:intron 25.0%
!! TCATTGTTATACCCTAATTT+TGG - Chr1:36053795-36053814 None:intergenic 25.0%
!! TGTTGTTGATCTGAAATATT+TGG - Chr1:36051381-36051400 None:intergenic 25.0%
!!! ACTGAAAGAAATTTTAACAC+AGG + Chr1:36053495-36053514 MsG0180002271.01.T01:intron 25.0%
!!! CATTCAACTCATTTTAATCT+TGG - Chr1:36053632-36053651 None:intergenic 25.0%
!!! CTGGAGAATTATTTTTACTT+TGG - Chr1:36051673-36051692 None:intergenic 25.0%
!!! CTTTGAAGTATAAGGATTAT+AGG - Chr1:36051205-36051224 None:intergenic 25.0%
!!! GTATTTTTAGGTCCAAAATT+AGG + Chr1:36053780-36053799 MsG0180002271.01.T01:intron 25.0%
AAAAAATCATCACCAATGCA+AGG + Chr1:36051168-36051187 MsG0180002271.01.T01:intron 30.0%
AGAATTGCAGTTATTCACAA+AGG + Chr1:36054214-36054233 MsG0180002271.01.T01:CDS 30.0%
ATTAGGGTATAACAATGACA+AGG + Chr1:36053797-36053816 MsG0180002271.01.T01:intron 30.0%
CAAATGTAAAAGGAATCACA+AGG + Chr1:36051590-36051609 MsG0180002271.01.T01:intron 30.0%
CAAGATTGATCAAGAAATAC+TGG - Chr1:36051692-36051711 None:intergenic 30.0%
CTTGGTTCATATCAAAAAAC+GGG + Chr1:36052255-36052274 MsG0180002271.01.T01:intron 30.0%
GAATTGAAGGGAAAATTCTT+TGG + Chr1:36054128-36054147 MsG0180002271.01.T01:CDS 30.0%
TAATTTCCTTGACTATTGGA+TGG - Chr1:36054283-36054302 None:intergenic 30.0%
TATCTTGGCTCATGAATAAT+TGG - Chr1:36051433-36051452 None:intergenic 30.0%
TATTCATGAGCCAAGATATT+GGG + Chr1:36051435-36051454 MsG0180002271.01.T01:intron 30.0%
TGGTAAATGAATGAAAAGTG+TGG + Chr1:36052888-36052907 MsG0180002271.01.T01:intron 30.0%
TGGTTAGAGAAGTTAGTTAT+GGG + Chr1:36052800-36052819 MsG0180002271.01.T01:intron 30.0%
TGTAAAAGATCACAAGAAAC+AGG + Chr1:36051551-36051570 MsG0180002271.01.T01:intron 30.0%
TGTTCGATTGTGATTGAATT+CGG + Chr1:36052532-36052551 MsG0180002271.01.T01:intron 30.0%
TTACTCCATTTGTATAGCTT+TGG - Chr1:36054248-36054267 None:intergenic 30.0%
TTATTCATGAGCCAAGATAT+TGG + Chr1:36051434-36051453 MsG0180002271.01.T01:intron 30.0%
TTTGACATGAGATTGAAACA+AGG + Chr1:36053588-36053607 MsG0180002271.01.T01:intron 30.0%
! ATGCAAATAATAAAAGTGCG+AGG + Chr1:36053066-36053085 MsG0180002271.01.T01:intron 30.0%
! GATTAAAATGAGTTGAATGC+AGG + Chr1:36053633-36053652 MsG0180002271.01.T01:intron 30.0%
! GCAAATTGTTGATCTTTTGA+AGG - Chr1:36054308-36054327 None:intergenic 30.0%
! TAATTTGTGAAATCAGTGTG+TGG + Chr1:36054013-36054032 MsG0180002271.01.T01:intron 30.0%
! TGCATGTTTGTGTATTGTTT+TGG - Chr1:36052197-36052216 None:intergenic 30.0%
! TTTTAGTCAATTTAGCCCTA+GGG - Chr1:36053536-36053555 None:intergenic 30.0%
!! GAAGGTTGTTGAATTTGTTA+TGG + Chr1:36052713-36052732 MsG0180002271.01.T01:intron 30.0%
!! TTTTTAGTCAATTTAGCCCT+AGG - Chr1:36053537-36053556 None:intergenic 30.0%
!!! TGCATTGGTGATGATTTTTT+AGG - Chr1:36051168-36051187 None:intergenic 30.0%
AAAAGACTAAAATGCCCCTA+GGG + Chr1:36053360-36053379 MsG0180002271.01.T01:intron 35.0%
AAACACGTTTGAGAGAAAGA+GGG - Chr1:36051984-36052003 None:intergenic 35.0%
AAATGACTAAAATGCCCCTA+GGG + Chr1:36053518-36053537 MsG0180002271.01.T01:intron 35.0%
AACATATCGAAGCAAAGAAG+CGG - Chr1:36052369-36052388 None:intergenic 35.0%
ACAGATGTCATAATAGACAC+TGG - Chr1:36053931-36053950 None:intergenic 35.0%
ACCAATAACATTTGGACACA+TGG + Chr1:36053009-36053028 MsG0180002271.01.T01:intron 35.0%
ACGTGGGAAAAAAGAAAAGA+GGG + Chr1:36052836-36052855 MsG0180002271.01.T01:intron 35.0%
ATAGATGATGTGGTAAAGCT+TGG + Chr1:36052936-36052955 MsG0180002271.01.T01:intron 35.0%
ATCTTTAGTGAAAGAGGGAA+AGG - Chr1:36052007-36052026 None:intergenic 35.0%
ATGAAAGTGGAGAAAGAGAT+GGG + Chr1:36051008-36051027 MsG0180002271.01.T01:CDS 35.0%
ATTCATGAGCCAAGATATTG+GGG + Chr1:36051436-36051455 MsG0180002271.01.T01:intron 35.0%
ATTCGAAGATTCGTGCAATT+CGG + Chr1:36052436-36052455 MsG0180002271.01.T01:intron 35.0%
CAAACAAAACTTGGAACACA+AGG + Chr1:36051329-36051348 MsG0180002271.01.T01:intron 35.0%
GGCATAATTTCCTTGACTAT+TGG - Chr1:36054287-36054306 None:intergenic 35.0%
GGTTAGAGAAGTTAGTTATG+GGG + Chr1:36052801-36052820 MsG0180002271.01.T01:intron 35.0%
GTGGTTAGAGAAGTTAGTTA+TGG + Chr1:36052799-36052818 MsG0180002271.01.T01:intron 35.0%
TAACAACCATCCAATAGTCA+AGG + Chr1:36054274-36054293 MsG0180002271.01.T01:CDS 35.0%
TCATCGCATACAAAGCTAAT+TGG + Chr1:36052304-36052323 MsG0180002271.01.T01:intron 35.0%
TGACCCATTTATAGATGATG+TGG + Chr1:36052926-36052945 MsG0180002271.01.T01:intron 35.0%
TTGAAGGGAAAATTCTTTGG+AGG + Chr1:36054131-36054150 MsG0180002271.01.T01:CDS 35.0%
TTGAGGTCGAATTCATATAG+AGG - Chr1:36052168-36052187 None:intergenic 35.0%
TTTAGTCAATTTAGCCCTAG+GGG - Chr1:36053535-36053554 None:intergenic 35.0%
TTTCTCGCGTTCTTTCTTAT+CGG - Chr1:36054066-36054085 None:intergenic 35.0%
! CCTTCTTCTTTTTCTCTATC+CGG + Chr1:36052482-36052501 MsG0180002271.01.T01:intron 35.0%
! CTGCTACTTCTTGGAATAAA+GGG - Chr1:36054196-36054215 None:intergenic 35.0%
! TCTGCTACTTCTTGGAATAA+AGG - Chr1:36054197-36054216 None:intergenic 35.0%
! TTGAAGTTTGCGTTTGTTGA+TGG + Chr1:36052608-36052627 MsG0180002271.01.T01:intron 35.0%
!! AAAGGACTTTTTGAAAGTGC+AGG + Chr1:36051778-36051797 MsG0180002271.01.T01:intron 35.0%
!! AAGGACTTTTTGAAAGTGCA+GGG + Chr1:36051779-36051798 MsG0180002271.01.T01:intron 35.0%
!! CATTTTAACCCCCAATATCT+TGG - Chr1:36051448-36051467 None:intergenic 35.0%
!! GGTTGTTGAATTTGTTATGG+TGG + Chr1:36052716-36052735 MsG0180002271.01.T01:intron 35.0%
!! TGGAAACCCTTTGAAGTATA+AGG - Chr1:36051213-36051232 None:intergenic 35.0%
!! TGTTGAATTTGTTATGGTGG+CGG + Chr1:36052719-36052738 MsG0180002271.01.T01:intron 35.0%
!!! TTAGAGGAGTTTTGAAACTC+CGG - Chr1:36052107-36052126 None:intergenic 35.0%
AAAGACTAAAATGCCCCTAG+GGG + Chr1:36053361-36053380 MsG0180002271.01.T01:intron 40.0%
AAGAAACGCTACCTTGAGTT+CGG + Chr1:36052330-36052349 MsG0180002271.01.T01:intron 40.0%
ACAAAATCAGTACAACACGC+AGG + Chr1:36051815-36051834 MsG0180002271.01.T01:intron 40.0%
ACATATCGAAGCAAAGAAGC+GGG - Chr1:36052368-36052387 None:intergenic 40.0%
ACCAATTGGAATGAGACATG+TGG + Chr1:36052959-36052978 MsG0180002271.01.T01:intron 40.0%
AGCGATTTCGAAACAGATTC+CGG - Chr1:36052504-36052523 None:intergenic 40.0%
CAAAATCAGTACAACACGCA+GGG + Chr1:36051816-36051835 MsG0180002271.01.T01:intron 40.0%
CACGTGGGAAAAAAGAAAAG+AGG + Chr1:36052835-36052854 MsG0180002271.01.T01:intron 40.0%
CCGGATAGAGAAAAAGAAGA+AGG - Chr1:36052485-36052504 None:intergenic 40.0%
GAAAAGACTAAAATGCCCCT+AGG + Chr1:36053359-36053378 MsG0180002271.01.T01:intron 40.0%
GAAACACGTTTGAGAGAAAG+AGG - Chr1:36051985-36052004 None:intergenic 40.0%
GAAATGACTAAAATGCCCCT+AGG + Chr1:36053517-36053536 MsG0180002271.01.T01:intron 40.0%
GAGAAAGAGGGAAAGAATGA+AGG - Chr1:36051972-36051991 None:intergenic 40.0%
GCACGAATCTTCGAATCAAA+AGG - Chr1:36052432-36052451 None:intergenic 40.0%
GCCATGTGTCCAAATGTTAT+TGG - Chr1:36053013-36053032 None:intergenic 40.0%
GCTGCATCTTTAGTGAAAGA+GGG - Chr1:36052012-36052031 None:intergenic 40.0%
GGTCGAATTCATATAGAGGA+AGG - Chr1:36052164-36052183 None:intergenic 40.0%
GTCATATTCAAGCCTTGCAT+TGG - Chr1:36051183-36051202 None:intergenic 40.0%
TCGCATACAAAGCTAATTGG+TGG + Chr1:36052307-36052326 MsG0180002271.01.T01:intron 40.0%
TTCATATAGAGGAAGGAGAG+AGG - Chr1:36052157-36052176 None:intergenic 40.0%
TTCATGAGCCAAGATATTGG+GGG + Chr1:36051437-36051456 MsG0180002271.01.T01:intron 40.0%
TTCTCGCGTTCTTTCTTATC+GGG - Chr1:36054065-36054084 None:intergenic 40.0%
! AAGTTTGCGTTTGTTGATGG+AGG + Chr1:36052611-36052630 MsG0180002271.01.T01:intron 40.0%
! ACTGCAATTCTGCTACTTCT+TGG - Chr1:36054205-36054224 None:intergenic 40.0%
! CATGGGTTAATGGGTCAAAT+AGG + Chr1:36051914-36051933 MsG0180002271.01.T01:intron 40.0%
! CTCTTTTCTTTTTTCCCACG+TGG - Chr1:36052837-36052856 None:intergenic 40.0%
! GGTGCTTGAAGAGCAATTTT+GGG - Chr1:36051412-36051431 None:intergenic 40.0%
! GGTTAATGGGTCAAATAGGA+GGG + Chr1:36051918-36051937 MsG0180002271.01.T01:intron 40.0%
! GTGCTTGAAGAGCAATTTTG+GGG - Chr1:36051411-36051430 None:intergenic 40.0%
! TGGTGCTTGAAGAGCAATTT+TGG - Chr1:36051413-36051432 None:intergenic 40.0%
! TTCTTTGGAGGAGAAGAGAT+TGG + Chr1:36054143-36054162 MsG0180002271.01.T01:CDS 40.0%
! TTGAGTCAATTTTGCCCCTA+GGG - Chr1:36053378-36053397 None:intergenic 40.0%
! TTTGAGTCAATTTTGCCCCT+AGG - Chr1:36053379-36053398 None:intergenic 40.0%
! TTTGTTGATGGAGGAGGTTA+CGG + Chr1:36052620-36052639 MsG0180002271.01.T01:intron 40.0%
!! AGGACTTTTTGAAAGTGCAG+GGG + Chr1:36051780-36051799 MsG0180002271.01.T01:intron 40.0%
!! GAAAAGAGAGTGTTGAATGG+TGG + Chr1:36052664-36052683 MsG0180002271.01.T01:intron 40.0%
!! GGAGAAAAGAGAGTGTTGAA+TGG + Chr1:36052661-36052680 MsG0180002271.01.T01:intron 40.0%
!! TTAGTCGTTTTAAGCCTCCA+GGG - Chr1:36053221-36053240 None:intergenic 40.0%
!! TTTAGTCGTTTTAAGCCTCC+AGG - Chr1:36053222-36053241 None:intergenic 40.0%
!!! GACACTTTTTCACGTGTTCT+TGG + Chr1:36052237-36052256 MsG0180002271.01.T01:intron 40.0%
AATTCGTTGGGCCTAAGAGT+TGG + Chr1:36051879-36051898 MsG0180002271.01.T01:intron 45.0%
ACATTTGGACACATGGCACT+TGG + Chr1:36053016-36053035 MsG0180002271.01.T01:intron 45.0%
ACCCTATCCTTCATCTTCTC+CGG + Chr1:36052035-36052054 MsG0180002271.01.T01:intron 45.0%
ACCGGAAAACAAGTTTCCAC+CGG - Chr1:36052057-36052076 None:intergenic 45.0%
ACCGGAGAAGATGAAGGATA+GGG - Chr1:36052039-36052058 None:intergenic 45.0%
AGCTAGCGACGAACTATTGA+AGG + Chr1:36054085-36054104 MsG0180002271.01.T01:CDS 45.0%
ATCAAAAGGAAGAAGCAGCG+TGG - Chr1:36052418-36052437 None:intergenic 45.0%
CAGTTCAAGCGGTGAAAAGA+AGG - Chr1:36052392-36052411 None:intergenic 45.0%
CGCTGCATCTTTAGTGAAAG+AGG - Chr1:36052013-36052032 None:intergenic 45.0%
CTAAGAGTTGGAGCAGGTAT+GGG + Chr1:36051891-36051910 MsG0180002271.01.T01:intron 45.0%
GATTGAGGTAGTTAGGGTGA+TGG + Chr1:36052688-36052707 MsG0180002271.01.T01:intron 45.0%
GCATCAACGCTTCAGATTGA+AGG + Chr1:36053883-36053902 MsG0180002271.01.T01:intron 45.0%
GCCACATGTCTCATTCCAAT+TGG - Chr1:36052963-36052982 None:intergenic 45.0%
GCGTGAAACAGAGGAGAAAT+TGG - Chr1:36052463-36052482 None:intergenic 45.0%
GCGTTCGTTGATGTTGTTGA+TGG + Chr1:36052568-36052587 MsG0180002271.01.T01:intron 45.0%
GGTGGTGATTGAGGTAGTTA+GGG + Chr1:36052682-36052701 MsG0180002271.01.T01:intron 45.0%
GTGGTAAAGCTTGGACCAAT+TGG + Chr1:36052945-36052964 MsG0180002271.01.T01:intron 45.0%
TTACGGTGGGGAAAGAAAGA+AGG + Chr1:36052637-36052656 MsG0180002271.01.T01:intron 45.0%
! AAGAAGAAGGCGTGAAACAG+AGG - Chr1:36052472-36052491 None:intergenic 45.0%
! AGGTATGGGCATGGGTTAAT+GGG + Chr1:36051905-36051924 MsG0180002271.01.T01:intron 45.0%
! CTTCAGATTGAAGGCATGTC+TGG + Chr1:36053892-36053911 MsG0180002271.01.T01:intron 45.0%
! GGGTTAATGGGTCAAATAGG+AGG + Chr1:36051917-36051936 MsG0180002271.01.T01:intron 45.0%
! GTGTTGAATGGTGGTGATTG+AGG + Chr1:36052673-36052692 MsG0180002271.01.T01:intron 45.0%
! GTTTGTTAGGGAGGAGGAAT+GGG + Chr1:36052746-36052765 MsG0180002271.01.T01:intron 45.0%
! TGAGTCAATTTTGCCCCTAG+GGG - Chr1:36053377-36053396 None:intergenic 45.0%
! TGGTGGTGATTGAGGTAGTT+AGG + Chr1:36052681-36052700 MsG0180002271.01.T01:intron 45.0%
! TTTGCGTTTGTTGATGGAGG+AGG + Chr1:36052614-36052633 MsG0180002271.01.T01:intron 45.0%
!! AGTTTGTTAGGGAGGAGGAA+TGG + Chr1:36052745-36052764 MsG0180002271.01.T01:intron 45.0%
!! GTAGTTAGGGTGATGGAAGA+AGG + Chr1:36052695-36052714 MsG0180002271.01.T01:intron 45.0%
!! GTTTGTTGTGGTGGTGGAAT+GGG + Chr1:36052777-36052796 MsG0180002271.01.T01:intron 45.0%
!! TAGTCGTTTTAAGCCTCCAG+GGG - Chr1:36053220-36053239 None:intergenic 45.0%
!! TCCGGTGGAAACTTGTTTTC+CGG + Chr1:36052053-36052072 MsG0180002271.01.T01:intron 45.0%
!! TGAATTTGTTATGGTGGCGG+AGG + Chr1:36052722-36052741 MsG0180002271.01.T01:intron 45.0%
!! TGGAAACTTGTTTTCCGGTG+CGG + Chr1:36052058-36052077 MsG0180002271.01.T01:intron 45.0%
!!! GTGGTTTTGGTTTGTTGTGG+TGG + Chr1:36052768-36052787 MsG0180002271.01.T01:intron 45.0%
!!! GTTTTGGTTTGTTGTGGTGG+TGG + Chr1:36052771-36052790 MsG0180002271.01.T01:intron 45.0%
!!! TGGGTGGTTTTGGTTTGTTG+TGG + Chr1:36052765-36052784 MsG0180002271.01.T01:intron 45.0%
ATCAGTACAACACGCAGGGA+AGG + Chr1:36051820-36051839 MsG0180002271.01.T01:intron 50.0%
CACCGGAGAAGATGAAGGAT+AGG - Chr1:36052040-36052059 None:intergenic 50.0%
CAGCGTGGAATCAGTTCAAG+CGG - Chr1:36052403-36052422 None:intergenic 50.0%
CCATACCTGCTCCAACTCTT+AGG - Chr1:36051893-36051912 None:intergenic 50.0%
CCTAAGAGTTGGAGCAGGTA+TGG + Chr1:36051890-36051909 MsG0180002271.01.T01:intron 50.0%
CGCTACCTTGAGTTCGGATT+TGG + Chr1:36052336-36052355 MsG0180002271.01.T01:intron 50.0%
CTATCCTTCATCTTCTCCGG+TGG + Chr1:36052038-36052057 MsG0180002271.01.T01:intron 50.0%
CTGCACCAAATCCGAACTCA+AGG - Chr1:36052344-36052363 None:intergenic 50.0%
GGAGAAGATGAAGGATAGGG+TGG - Chr1:36052036-36052055 None:intergenic 50.0%
GTGGAGAAAGAGATGGGATG+TGG + Chr1:36051014-36051033 MsG0180002271.01.T01:CDS 50.0%
GTTTCCACCGGAGAAGATGA+AGG - Chr1:36052045-36052064 None:intergenic 50.0%
TCAGTACAACACGCAGGGAA+GGG + Chr1:36051821-36051840 MsG0180002271.01.T01:intron 50.0%
TGAAATGACCAAAACGCCCC+TGG + Chr1:36053201-36053220 MsG0180002271.01.T01:intron 50.0%
TTGATGGAGGAGGTTACGGT+GGG + Chr1:36052624-36052643 MsG0180002271.01.T01:intron 50.0%
! CAGGTATGGGCATGGGTTAA+TGG + Chr1:36051904-36051923 MsG0180002271.01.T01:intron 50.0%
!! AAACTTGTTTTCCGGTGCGG+TGG + Chr1:36052061-36052080 MsG0180002271.01.T01:intron 50.0%
!! GGTTTGTTGTGGTGGTGGAA+TGG + Chr1:36052776-36052795 MsG0180002271.01.T01:intron 50.0%
!!! GGAGTTTTGAAACTCCGGTG+CGG - Chr1:36052102-36052121 None:intergenic 50.0%
AATGACCAAAACGCCCCTGG+AGG + Chr1:36053204-36053223 MsG0180002271.01.T01:intron 55.0%
AGTACAACACGCAGGGAAGG+GGG + Chr1:36051823-36051842 MsG0180002271.01.T01:intron 55.0%
AGTTGGAGCAGGTATGGGCA+TGG + Chr1:36051896-36051915 MsG0180002271.01.T01:intron 55.0%
ATCAGTGTGTGGCTGCTGCA+TGG + Chr1:36054024-36054043 MsG0180002271.01.T01:intron 55.0%
CAGTACAACACGCAGGGAAG+GGG + Chr1:36051822-36051841 MsG0180002271.01.T01:intron 55.0%
CGGTGGGGAAAGAAAGAAGG+AGG + Chr1:36052640-36052659 MsG0180002271.01.T01:intron 55.0%
GTTGGAGCAGGTATGGGCAT+GGG + Chr1:36051897-36051916 MsG0180002271.01.T01:intron 55.0%
TGATGGAGGAGGTTACGGTG+GGG + Chr1:36052625-36052644 MsG0180002271.01.T01:intron 55.0%
TGTGGCTGCTGCATGGATGT+CGG + Chr1:36054031-36054050 MsG0180002271.01.T01:CDS 55.0%
TGTTAGGGAGGAGGAATGGG+TGG + Chr1:36052749-36052768 MsG0180002271.01.T01:intron 55.0%
TTGGGCCTAAGAGTTGGAGC+AGG + Chr1:36051885-36051904 MsG0180002271.01.T01:intron 55.0%
! GGAGGAGGAATGGGTGGTTT+TGG + Chr1:36052755-36052774 MsG0180002271.01.T01:intron 55.0%
! GGGGGGTGTGAAAAGCATAC+TGG + Chr1:36051842-36051861 MsG0180002271.01.T01:intron 55.0%
! GTTGATGGAGGAGGTTACGG+TGG + Chr1:36052623-36052642 MsG0180002271.01.T01:intron 55.0%
!! GGAGGAGTTTGTTAGGGAGG+AGG + Chr1:36052740-36052759 MsG0180002271.01.T01:intron 55.0%
!! GGTGGCGGAGGAGTTTGTTA+GGG + Chr1:36052734-36052753 MsG0180002271.01.T01:intron 55.0%
!! TGGTGGCGGAGGAGTTTGTT+AGG + Chr1:36052733-36052752 MsG0180002271.01.T01:intron 55.0%
!! TGTTGTGGTGGTGGAATGGG+TGG + Chr1:36052780-36052799 MsG0180002271.01.T01:intron 55.0%
!!! GTTTTGAAACTCCGGTGCGG+TGG - Chr1:36052099-36052118 None:intergenic 55.0%
!!! TTAAGCCTCCAGGGGCGTTT+TGG - Chr1:36053212-36053231 None:intergenic 55.0%
GGGGAAGAAGAGAGCCACGT+GGG + Chr1:36052820-36052839 MsG0180002271.01.T01:intron 60.0%
GTACAACACGCAGGGAAGGG+GGG + Chr1:36051824-36051843 MsG0180002271.01.T01:intron 60.0%
TACAACACGCAGGGAAGGGG+GGG + Chr1:36051825-36051844 MsG0180002271.01.T01:intron 60.0%
TGGGGAAGAAGAGAGCCACG+TGG + Chr1:36052819-36052838 MsG0180002271.01.T01:intron 60.0%
! TTGTTTTCCGGTGCGGTGGC+CGG + Chr1:36052065-36052084 MsG0180002271.01.T01:intron 60.0%
!! GGCGGAGGAGTTTGTTAGGG+AGG + Chr1:36052737-36052756 MsG0180002271.01.T01:intron 60.0%
! TTTTCCGGTGCGGTGGCCGG+TGG + Chr1:36052068-36052087 MsG0180002271.01.T01:intron 70.0%
! CGGTGGTGGCACCACCGCAC+CGG + Chr1:36052085-36052104 MsG0180002271.01.T01:intron 75.0%
!! CGGTGCGGTGGTGCCACCAC+CGG - Chr1:36052087-36052106 None:intergenic 75.0%
! GCCACCACCGGCCACCGCAC+CGG - Chr1:36052075-36052094 None:intergenic 80.0%
! TCCGGTGCGGTGGCCGGTGG+TGG + Chr1:36052071-36052090 MsG0180002271.01.T01:intron 80.0%
Chromosome Type Strat End Strand Name
Chr1 gene 36051008 36054373 36051008 ID=MsG0180002271.01;Name=MsG0180002271.01
Chr1 mRNA 36051008 36054373 36051008 ID=MsG0180002271.01.T01;Parent=MsG0180002271.01;Name=MsG0180002271.01.T01;_AED=0.46;_eAED=0.59;_QI=0|0|0|1|1|1|2|0|150
Chr1 exon 36051008 36051115 36051008 ID=MsG0180002271.01.T01:exon:3282;Parent=MsG0180002271.01.T01
Chr1 exon 36054029 36054373 36054029 ID=MsG0180002271.01.T01:exon:3283;Parent=MsG0180002271.01.T01
Chr1 CDS 36051008 36051115 36051008 ID=MsG0180002271.01.T01:cds;Parent=MsG0180002271.01.T01
Chr1 CDS 36054029 36054373 36054029 ID=MsG0180002271.01.T01:cds;Parent=MsG0180002271.01.T01
Gene Sequence

>MsG0180002271.01.T01

ATGAAAGTGGAGAAAGAGATGGGATGTGGAATGCTTATGATGTATGAAACAATGAACAAATGGTTAGTAGCACAAGATATAAAGCAAGTCAAAGCTATCATAACAAGATGTGTGGCTGCTGCATGGATGTCGGTTTTTATGCCCGATAAGAAAGAACGCGAGAAAGCTAGCGACGAACTATTGAAGGCTCTACAATTTCTTGAGAATGAATTGAAGGGAAAATTCTTTGGAGGAGAAGAGATTGGAATTGTTGATATTGCTGCTCTCTTCATCCCTTTATTCCAAGAAGTAGCAGAATTGCAGTTATTCACAAAGGAGAAATTTCCAAAGCTATACAAATGGAGTAAAGAATTTAACAACCATCCAATAGTCAAGGAAATTATGCCTTCAAAAGATCAACAATTTGCATATTTCAAAGCTCGCGCTGAAAGCCTTGCTGCTTCATCAAAATAG
Protein sequence

>MsG0180002271.01.T01

MKVEKEMGCGMLMMYETMNKWLVAQDIKQVKAIITRCVAAAWMSVFMPDKKEREKASDELLKALQFLENELKGKFFGGEEIGIVDIAALFIPLFQEVAELQLFTKEKFPKLYKWSKEFNNHPIVKEIMPSKDQQFAYFKARAESLAASSK*