AlfalfaGEDB | MsG0580026309.01

M. sativa cultivar ZhongmuNo.1 / MsG0580026309.01

KEGG: -
GO: -
PFAM: PF03763

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026309.01.T01	PNX73788.1	52.846	123	26	1	6	128	215	305	4.17E-28	115

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026309.01.T01	A0A2K3L5H9	52.846	123	26	1	6	128	215	305	1.99e-28	115

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0580026309.01	0.814524	1.474278e-51	9.915786e-49
MsG0080048113.01	MsG0580026309.01	0.801300	9.610306e-49	4.573628e-46
MsG0080048856.01	MsG0580026309.01	0.824361	8.414492e-54	7.436628e-51
MsG0180000024.01	MsG0580026309.01	0.807971	3.894943e-50	2.199761e-47
MsG0180000139.01	MsG0580026309.01	0.800657	1.300684e-48	6.090096e-46
MsG0180000983.01	MsG0580026309.01	0.816321	5.873841e-52	4.149032e-49
MsG0180002271.01	MsG0580026309.01	0.801011	1.100976e-48	5.201926e-46
MsG0180003669.01	MsG0580026309.01	0.811217	7.820114e-51	4.812507e-48
MsG0180003911.01	MsG0580026309.01	0.814211	1.729192e-51	1.153115e-48
MsG0180004099.01	MsG0580026309.01	0.850408	1.730889e-60	3.387412e-57
MsG0180004389.01	MsG0580026309.01	0.823771	1.157722e-53	1.006390e-50
MsG0180004481.01	MsG0580026309.01	0.804864	1.760273e-49	9.177067e-47
MsG0180004482.01	MsG0580026309.01	0.806248	9.019933e-50	4.872319e-47
MsG0180004754.01	MsG0580026309.01	0.823471	1.360992e-53	1.172897e-50
MsG0180004970.01	MsG0580026309.01	0.806226	9.116402e-50	4.921691e-47
MsG0180005250.01	MsG0580026309.01	0.804339	2.264982e-49	1.164862e-46
MsG0180005393.01	MsG0580026309.01	0.823791	1.145021e-53	9.958812e-51
MsG0180005575.01	MsG0580026309.01	0.812578	3.951606e-51	2.521786e-48
MsG0180006024.01	MsG0580026309.01	0.828638	8.041603e-55	8.039681e-52
MsG0180006155.01	MsG0580026309.01	0.800024	1.750079e-48	8.063500e-46
MsG0280006299.01	MsG0580026309.01	0.806142	9.494849e-50	5.114867e-47
MsG0280010778.01	MsG0580026309.01	0.801786	7.639355e-49	3.680513e-46
MsG0280010907.01	MsG0580026309.01	0.829000	6.574490e-55	6.642135e-52
MsG0280011013.01	MsG0580026309.01	0.801365	9.318045e-49	4.441917e-46
MsG0280011014.01	MsG0580026309.01	0.809807	1.576361e-50	9.346490e-48
MsG0280011258.01	MsG0580026309.01	0.823803	1.138023e-53	9.900993e-51
MsG0280011282.01	MsG0580026309.01	0.826428	2.727509e-54	2.557280e-51
MsG0280011472.01	MsG0580026309.01	0.804561	2.036644e-49	1.053405e-46
MsG0380011640.01	MsG0580026309.01	0.803817	2.908858e-49	1.476099e-46
MsG0380011648.01	MsG0580026309.01	0.828204	1.023945e-54	1.010853e-51
MsG0380012460.01	MsG0580026309.01	0.807626	4.611432e-50	2.581040e-47
MsG0380012471.01	MsG0580026309.01	0.811068	8.421904e-51	5.162642e-48
MsG0380013475.01	MsG0580026309.01	0.822041	2.928486e-53	2.423392e-50
MsG0380013549.01	MsG0580026309.01	0.818704	1.706175e-52	1.286496e-49
MsG0380013834.01	MsG0580026309.01	0.800980	1.116985e-48	5.273404e-46
MsG0380014537.01	MsG0580026309.01	0.802754	4.828780e-49	2.384551e-46
MsG0480018894.01	MsG0580026309.01	0.801430	9.037663e-49	4.315122e-46
MsG0480019710.01	MsG0580026309.01	0.804159	2.469256e-49	1.264074e-46
MsG0480020739.01	MsG0580026309.01	0.809544	1.795847e-50	1.057382e-47
MsG0480020780.01	MsG0580026309.01	0.818558	1.840844e-52	1.382501e-49
MsG0480021465.01	MsG0580026309.01	0.810187	1.305436e-50	7.816954e-48
MsG0480022620.01	MsG0580026309.01	0.808163	3.544323e-50	2.012051e-47
MsG0480023130.01	MsG0580026309.01	0.805979	1.027463e-49	5.512104e-47
MsG0480023463.01	MsG0580026309.01	0.810670	1.027140e-50	6.230096e-48
MsG0480023873.01	MsG0580026309.01	0.824999	5.951766e-54	5.357951e-51
MsG0580024506.01	MsG0580026309.01	0.823840	1.115299e-53	9.713341e-51
MsG0580026059.01	MsG0580026309.01	0.811785	5.884239e-51	3.675902e-48
MsG0380015190.01	MsG0580026309.01	0.800195	1.614877e-48	7.472958e-46
MsG0380015332.01	MsG0580026309.01	0.806415	8.316197e-50	4.511124e-47
MsG0380015555.01	MsG0580026309.01	0.808015	3.811288e-50	2.155053e-47
MsG0380015677.01	MsG0580026309.01	0.819345	1.219489e-52	9.358943e-50
MsG0380015805.01	MsG0580026309.01	0.818433	1.965692e-52	1.471287e-49
MsG0380015944.01	MsG0580026309.01	0.812763	3.599371e-51	2.308164e-48
MsG0380016765.01	MsG0580026309.01	0.817908	2.583246e-52	1.905609e-49
MsG0380016994.01	MsG0580026309.01	0.822860	1.889897e-53	1.600958e-50
MsG0380017391.01	MsG0580026309.01	0.812389	4.345708e-51	2.759560e-48
MsG0480018163.01	MsG0580026309.01	0.803962	2.713432e-49	1.382103e-46
MsG0480018313.01	MsG0580026309.01	0.826606	2.474419e-54	2.331536e-51
MsG0480018346.01	MsG0580026309.01	0.804126	2.508798e-49	1.283223e-46
MsG0580026307.01	MsG0580026309.01	0.811160	8.044892e-51	4.943327e-48
MsG0580026308.01	MsG0580026309.01	0.867488	1.319209e-65	4.614271e-62
MsG0580026309.01	MsG0580026593.01	0.811833	5.744629e-51	3.593231e-48
MsG0580026309.01	MsG0580027431.01	0.819598	1.067754e-52	8.253442e-50
MsG0580026309.01	MsG0580028176.01	0.801864	7.363514e-49	3.554813e-46
MsG0580026309.01	MsG0580029493.01	0.804132	2.501333e-49	1.279597e-46
MsG0580026309.01	MsG0580029640.01	0.830927	2.228546e-55	2.382133e-52
MsG0580026309.01	MsG0580029825.01	0.828235	1.006281e-54	9.942988e-52
MsG0580026309.01	MsG0680030447.01	0.822334	2.504117e-53	2.089838e-50
MsG0580026309.01	MsG0680031506.01	0.801217	9.992023e-49	4.745308e-46
MsG0580026309.01	MsG0680031549.01	0.801097	1.057271e-48	5.006251e-46
MsG0580026309.01	MsG0680034842.01	0.809031	2.313308e-50	1.343484e-47
MsG0580026309.01	MsG0680034890.01	0.841795	3.826223e-58	5.684701e-55
MsG0580026309.01	MsG0680035475.01	0.804178	2.447345e-49	1.253431e-46
MsG0580026309.01	MsG0680035573.01	0.828640	8.036293e-55	8.034703e-52
MsG0580026309.01	MsG0680035692.01	0.817543	3.122112e-52	2.280330e-49
MsG0580026309.01	MsG0780036719.01	0.806792	6.924418e-50	3.792707e-47
MsG0580026309.01	MsG0780036752.01	0.820755	5.799189e-53	4.629210e-50
MsG0580026309.01	MsG0780036815.01	0.803529	3.337728e-49	1.681305e-46
MsG0580026309.01	MsG0780037054.01	0.800535	1.376956e-48	6.427205e-46
MsG0580026309.01	MsG0780038424.01	0.813040	3.130594e-51	2.022372e-48
MsG0580026309.01	MsG0780038691.01	0.805613	1.226839e-49	6.519808e-47
MsG0580026309.01	MsG0780038935.01	0.808106	3.645916e-50	2.066480e-47
MsG0580026309.01	MsG0780039243.01	0.810876	9.270244e-51	5.653952e-48
MsG0580026309.01	MsG0780039257.01	0.801037	1.087565e-48	5.141853e-46
MsG0580026309.01	MsG0780039443.01	0.803336	3.659343e-49	1.834055e-46
MsG0580026309.01	MsG0780041305.01	0.804493	2.103583e-49	1.086128e-46
MsG0580026309.01	MsG0880041860.01	0.801145	1.033866e-48	4.901147e-46
MsG0580026309.01	MsG0880041896.01	0.863684	2.088435e-64	6.386265e-61
MsG0580026309.01	MsG0880041986.01	0.811239	7.733406e-51	4.761919e-48
MsG0580026309.01	MsG0880042310.01	0.805429	1.340489e-49	7.090324e-47
MsG0580026309.01	MsG0880042311.01	0.855362	6.640320e-62	1.529360e-58
MsG0580026309.01	MsG0880042939.01	0.803834	2.885753e-49	1.465008e-46
MsG0580026309.01	MsG0880043034.01	0.807278	5.464035e-50	3.030858e-47
MsG0580026309.01	MsG0880043479.01	0.825236	5.233373e-54	4.742811e-51
MsG0580026309.01	MsG0880044782.01	0.814018	1.907484e-51	1.265437e-48
MsG0580026309.01	MsG0880046012.01	0.842392	2.660163e-58	4.027932e-55
MsG0580026309.01	MsG0880047473.01	0.812849	3.446254e-51	2.214897e-48
MsG0580026309.01	MsG0880047666.01	0.813487	2.496407e-51	1.632532e-48
MsG0280006567.01	MsG0580026309.01	0.802084	6.633521e-49	3.220325e-46
MsG0280006591.01	MsG0580026309.01	0.800355	1.498746e-48	6.963913e-46
MsG0280006910.01	MsG0580026309.01	0.807990	3.859176e-50	2.180694e-47
MsG0280007955.01	MsG0580026309.01	0.801100	1.055797e-48	4.999652e-46
MsG0280008615.01	MsG0580026309.01	0.816082	6.643217e-52	4.661849e-49
MsG0280008909.01	MsG0580026309.01	0.834771	2.471242e-56	2.961804e-53
MsG0280009514.01	MsG0580026309.01	0.803324	3.680693e-49	1.844158e-46
MsG0280009794.01	MsG0580026309.01	0.812778	3.571501e-51	2.291164e-48
MsG0280010489.01	MsG0580026309.01	0.812185	4.813739e-51	3.039927e-48
MsG0280010491.01	MsG0580026309.01	0.831650	1.480491e-55	1.616704e-52

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026309.01.T01	MTR_5g043690	53.659	123	25	1	6	128	222	312	8.91e-32	114
MsG0580026309.01.T01	MTR_5g043690	53.659	123	25	1	6	128	223	313	9.47e-32	114
MsG0580026309.01.T01	MTR_5g043685	57.600	125	38	4	6	121	156	274	2.71e-27	102
MsG0580026309.01.T01	MTR_4g082910	38.211	123	45	1	6	128	265	356	1.03e-12	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026309.01.T01	AT1G13920	36.800	125	46	2	5	128	286	378	1.84e-15	71.6
MsG0580026309.01.T01	AT1G13920	36.800	125	46	2	5	128	253	345	2.05e-15	71.2

Find 15 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAAGAAAGGTTGAAATCTT+TGG	0.219285	5:-34475015	MsG0580026309.01.T01:CDS
TTCCTTCACTAGCTTGCGTT+TGG	0.377600	5:+34475344	None:intergenic
GGAAAGAGTTGATAGATTCA+TGG	0.429602	5:-34475389	MsG0580026309.01.T01:CDS
GCAAGTACAATTTCAACAAC+AGG	0.459062	5:-34474789	MsG0580026309.01.T01:CDS
ATTCATGGGAAGACAAGAAA+AGG	0.490794	5:-34475374	MsG0580026309.01.T01:CDS
GAAAGAGTTGATAGATTCAT+GGG	0.495863	5:-34475388	MsG0580026309.01.T01:CDS
TGCAGAGTGAACTTGAAGAA+AGG	0.496563	5:-34475028	MsG0580026309.01.T01:intron
TATGTGAATCTAGTTGCAAA+AGG	0.505322	5:-34474870	MsG0580026309.01.T01:CDS
ACGCAAGCTAGTGAAGGAAC+AGG	0.521158	5:-34475340	MsG0580026309.01.T01:intron
TATGTAAAACTAGTTGCAAA+CGG	0.533130	5:-34474969	MsG0580026309.01.T01:CDS
ACATTGTCAGGTATGAGAAG+TGG	0.585278	5:-34475410	MsG0580026309.01.T01:CDS
AGCCAAACGCAAGCTAGTGA+AGG	0.599861	5:-34475346	MsG0580026309.01.T01:CDS
AGAGCTACAAGCAAAAGAGA+AGG	0.643295	5:-34474811	MsG0580026309.01.T01:CDS
TGCAAAAGGAGCGAGAGACA+AGG	0.645612	5:-34474856	MsG0580026309.01.T01:CDS
CAGAAGCAGAAATACGTCTG+TGG	0.688002	5:+34474759	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAAAAAAAACTAGAG+AGG	-	Chr5:34475055-34475074	MsG0580026309.01.T01:intron	15.0%
!	CTAAAATCAGATAATGTTCT+AGG	-	Chr5:34474941-34474960	MsG0580026309.01.T01:CDS	25.0%
!	TAGTAAGACACAAATTTAAC+AGG	+	Chr5:34474968-34474987	None:intergenic	25.0%
!	TATGTAAAACTAGTTGCAAA+CGG	-	Chr5:34475206-34475225	MsG0580026309.01.T01:intron	25.0%
!!!	ACATTATCTGATTTTAGCTA+AGG	+	Chr5:34474938-34474957	None:intergenic	25.0%
	AAAAAAAAAACTAGAGAGGC+CGG	-	Chr5:34475059-34475078	MsG0580026309.01.T01:intron	30.0%
	CATTTAATCCTTCTTTAAGC+AGG	-	Chr5:34474899-34474918	MsG0580026309.01.T01:CDS	30.0%
	TATGTGAATCTAGTTGCAAA+AGG	-	Chr5:34475305-34475324	MsG0580026309.01.T01:intron	30.0%
	TCTAGTATGAACAATAAGTC+TGG	-	Chr5:34475023-34475042	MsG0580026309.01.T01:CDS	30.0%
	TGAAGAAAGGTTGAAATCTT+TGG	-	Chr5:34475160-34475179	MsG0580026309.01.T01:intron	30.0%
!!	GAAAGAGTTGATAGATTCAT+GGG	-	Chr5:34474787-34474806	MsG0580026309.01.T01:CDS	30.0%
	AAAAAAAAACTAGAGAGGCC+GGG	-	Chr5:34475060-34475079	MsG0580026309.01.T01:intron	35.0%
	ATTCATGGGAAGACAAGAAA+AGG	-	Chr5:34474801-34474820	MsG0580026309.01.T01:CDS	35.0%
	GCAAGTACAATTTCAACAAC+AGG	-	Chr5:34475386-34475405	MsG0580026309.01.T01:CDS	35.0%
	TTTCATGACCTGCTTAAAGA+AGG	+	Chr5:34474910-34474929	None:intergenic	35.0%
!	TCTCTCAGACACTCTTTTTA+AGG	-	Chr5:34474860-34474879	MsG0580026309.01.T01:CDS	35.0%
!!	GGAAAGAGTTGATAGATTCA+TGG	-	Chr5:34474786-34474805	MsG0580026309.01.T01:CDS	35.0%
	ACATTGTCAGGTATGAGAAG+TGG	-	Chr5:34474765-34474784	MsG0580026309.01.T01:CDS	40.0%
	AGAGCTACAAGCAAAAGAGA+AGG	-	Chr5:34475364-34475383	MsG0580026309.01.T01:CDS	40.0%
	TGCAGAGTGAACTTGAAGAA+AGG	-	Chr5:34475147-34475166	MsG0580026309.01.T01:intron	40.0%
!!!	CTCCCACAACATTTTTTACC+CGG	+	Chr5:34475081-34475100	None:intergenic	40.0%
	AGGCCGGGTAAAAAATGTTG+TGG	-	Chr5:34475075-34475094	MsG0580026309.01.T01:intron	45.0%
	CAGAAGCAGAAATACGTCTG+TGG	+	Chr5:34475419-34475438	None:intergenic	45.0%
	GGCCGGGTAAAAAATGTTGT+GGG	-	Chr5:34475076-34475095	MsG0580026309.01.T01:intron	45.0%
!	TTCCTTCACTAGCTTGCGTT+TGG	+	Chr5:34474834-34474853	None:intergenic	45.0%
	ACGCAAGCTAGTGAAGGAAC+AGG	-	Chr5:34474835-34474854	MsG0580026309.01.T01:CDS	50.0%
	AGCCAAACGCAAGCTAGTGA+AGG	-	Chr5:34474829-34474848	MsG0580026309.01.T01:CDS	50.0%
	TGCAAAAGGAGCGAGAGACA+AGG	-	Chr5:34475319-34475338	MsG0580026309.01.T01:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	34474758	34475439	34474758	ID=MsG0580026309.01;Name=MsG0580026309.01
Chr5	mRNA	34474758	34475439	34474758	ID=MsG0580026309.01.T01;Parent=MsG0580026309.01;Name=MsG0580026309.01.T01;_AED=0.47;_eAED=0.57;_QI=0\|0\|0\|1\|1\|1\|2\|0\|128
Chr5	exon	34475341	34475439	34475341	ID=MsG0580026309.01.T01:exon:13945;Parent=MsG0580026309.01.T01
Chr5	exon	34474758	34475045	34474758	ID=MsG0580026309.01.T01:exon:13944;Parent=MsG0580026309.01.T01
Chr5	CDS	34475341	34475439	34475341	ID=MsG0580026309.01.T01:cds;Parent=MsG0580026309.01.T01
Chr5	CDS	34474758	34475045	34474758	ID=MsG0580026309.01.T01:cds;Parent=MsG0580026309.01.T01

Gene Sequence

>MsG0580026309.01.T01

ATGAAAAACATTGTCAGGTATGAGAAGTGGAAAGAGTTGATAGATTCATGGGAAGACAAGAAAAGGAAGAAAGCCAAACGCAAGCTAGTGAAGGAACAGAGTGAACTTGAAGAAAGGTTGAAATCTTTGGAGAAAATTCAAAATAAGATTAAGTATGTAAAACTAGTTGCAAACGGAGCATATGTCAAATTGCAGAGTGAAATTGATCTGCAAAAGTTGAAATCTGTGAAGAAATTTAATAATAAGATGAAGTATGTGAATCTAGTTGCAAAAGGAGCGAGAGACAAGGCTGAGAAAAGACGAGAAAATGAAGAGCTACAAGCAAAAGAGAAGGCAAGTACAATTTCAACAACAGGCACACTGCCACAGACGTATTTCTGCTTCTGA

Protein sequence

>MsG0580026309.01.T01

MKNIVRYEKWKELIDSWEDKKRKKAKRKLVKEQSELEERLKSLEKIQNKIKYVKLVANGAYVKLQSEIDLQKLKSVKKFNNKMKYVNLVAKGARDKAEKRRENEELQAKEKASTISTTGTLPQTYFCF*