Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002441.01.T01 | XP_039687607.1 | 98.734 | 79 | 1 | 0 | 1 | 79 | 325 | 403 | 7.41E-48 | 166 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002441.01.T01 | A0A1S4D1D3 | 70 | 80 | 23 | 1 | 1 | 79 | 324 | 403 | 2.90E-28 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002441.01.T01 | G7J7F3 | 98.734 | 79 | 1 | 0 | 1 | 79 | 326 | 404 | 3.90e-48 | 166 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048197.01 | MsG0180002441.01 | 0.822202 | 2.687681e-53 | 2.234450e-50 |
| MsG0180002441.01 | MsG0180002491.01 | 0.818210 | 2.207593e-52 | 1.642053e-49 |
| MsG0180002441.01 | MsG0280009235.01 | 0.803459 | 3.450452e-49 | 1.734902e-46 |
| MsG0180002441.01 | MsG0380015818.01 | 0.803504 | 3.378608e-49 | 1.700752e-46 |
| MsG0180002441.01 | MsG0380015922.01 | 0.812507 | 4.095309e-51 | 2.608693e-48 |
| MsG0180002441.01 | MsG0380017979.01 | 0.803700 | 3.076502e-49 | 1.556425e-46 |
| MsG0180002441.01 | MsG0580025134.01 | 0.810785 | 9.698600e-51 | 5.900735e-48 |
| MsG0180002441.01 | MsG0580027034.01 | 0.801831 | 7.479143e-49 | 3.607531e-46 |
| MsG0180002441.01 | MsG0580028900.01 | 0.801901 | 7.233785e-49 | 3.495444e-46 |
| MsG0180002441.01 | MsG0580029300.01 | 0.801248 | 9.849145e-49 | 4.681153e-46 |
| MsG0180002441.01 | MsG0780039340.01 | 0.801737 | 7.819628e-49 | 3.762513e-46 |
| MsG0180002441.01 | MsG0780039943.01 | 0.814319 | 1.636608e-51 | 1.094664e-48 |
| MsG0180002441.01 | MsG0880045869.01 | 0.803512 | 3.364417e-49 | 1.694000e-46 |
| MsG0180002441.01 | MsG0880046058.01 | 0.810169 | 1.317641e-50 | 7.886518e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002441.01.T01 | MTR_3g091640 | 98.734 | 79 | 1 | 0 | 1 | 79 | 326 | 404 | 9.89e-52 | 166 |
| MsG0180002441.01.T01 | MTR_1g050518 | 97.468 | 79 | 2 | 0 | 1 | 79 | 266 | 344 | 2.40e-51 | 163 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002441.01.T01 | AT5G53000 | 62.963 | 81 | 28 | 1 | 1 | 79 | 325 | 405 | 8.09e-25 | 95.5 |
Find 27 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGCTAGAGCTTTGGATGAT+TGG | 0.301964 | 1:+38420077 | MsG0180002441.01.T01:CDS |
| ATTGGCTTCTTCAATGAATC+TGG | 0.346117 | 1:-38419978 | None:intergenic |
| ATCATTGTGCCAAGATGAAT+TGG | 0.351143 | 1:-38419996 | None:intergenic |
| ATTCATCTTGGCACAATGAT+AGG | 0.398345 | 1:+38419999 | MsG0180002441.01.T01:CDS |
| TCATCCTCCTCCTCTTCACC+TGG | 0.428915 | 1:-38420031 | None:intergenic |
| GGAAGCAGGATTAAAAGAAA+TGG | 0.429020 | 1:+38419925 | MsG0180002441.01.T01:CDS |
| TTGAAGAAGCCAATTCATCT+TGG | 0.455058 | 1:+38419987 | MsG0180002441.01.T01:CDS |
| GATGATAATCCGCGGGGTGC+AGG | 0.460136 | 1:+38420103 | MsG0180002441.01.T01:CDS |
| TCCTGCTTCCTCAATGCTCA+TGG | 0.489749 | 1:-38419912 | None:intergenic |
| ACAGGATAGAGCTAGAGCTT+TGG | 0.535946 | 1:+38420069 | MsG0180002441.01.T01:CDS |
| AAATGGAAATAATGAATACA+TGG | 0.538640 | 1:+38419942 | MsG0180002441.01.T01:CDS |
| TAGAGCTTTGGATGATTGGA+AGG | 0.539728 | 1:+38420081 | MsG0180002441.01.T01:CDS |
| GAAGTTTAAGCCAGGTGAAG+AGG | 0.541846 | 1:+38420021 | MsG0180002441.01.T01:CDS |
| TAAGCCAGGTGAAGAGGAGG+AGG | 0.545443 | 1:+38420027 | MsG0180002441.01.T01:CDS |
| AATTCGAAGCTTACCCCTTG+TGG | 0.556002 | 1:+38420127 | MsG0180002441.01.T01:CDS |
| TGAAAATGATGATGCTGCAC+AGG | 0.564839 | 1:+38420051 | MsG0180002441.01.T01:CDS |
| TTGGAAGGATGATAATCCGC+GGG | 0.569064 | 1:+38420096 | MsG0180002441.01.T01:CDS |
| GTTTAAGCCAGGTGAAGAGG+AGG | 0.583570 | 1:+38420024 | MsG0180002441.01.T01:CDS |
| ACCATGAGCATTGAGGAAGC+AGG | 0.596794 | 1:+38419911 | None:intergenic |
| AATGATAGGAAGTTTAAGCC+AGG | 0.610210 | 1:+38420013 | MsG0180002441.01.T01:CDS |
| GATGCCAACCATGAGCATTG+AGG | 0.623143 | 1:+38419904 | None:intergenic |
| ATAGATTCAATTAGCCACAA+GGG | 0.641175 | 1:-38420141 | None:intergenic |
| TAGATTCAATTAGCCACAAG+GGG | 0.647522 | 1:-38420140 | None:intergenic |
| TTCGAATTTCCTGCACCCCG+CGG | 0.651439 | 1:-38420112 | None:intergenic |
| AATAGATTCAATTAGCCACA+AGG | 0.660921 | 1:-38420142 | None:intergenic |
| ATTGGAAGGATGATAATCCG+CGG | 0.675321 | 1:+38420095 | MsG0180002441.01.T01:CDS |
| TGGAAGGATGATAATCCGCG+GGG | 0.769686 | 1:+38420097 | MsG0180002441.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGGAAATAATGAATACA+TGG | + | Chr1:38419942-38419961 | MsG0180002441.01.T01:CDS | 20.0% |
| AATGATAGGAAGTTTAAGCC+AGG | + | Chr1:38420013-38420032 | MsG0180002441.01.T01:CDS | 35.0% | |
| ATCATTGTGCCAAGATGAAT+TGG | - | Chr1:38419999-38420018 | None:intergenic | 35.0% | |
| ATTCATCTTGGCACAATGAT+AGG | + | Chr1:38419999-38420018 | MsG0180002441.01.T01:CDS | 35.0% | |
| GGAAGCAGGATTAAAAGAAA+TGG | + | Chr1:38419925-38419944 | MsG0180002441.01.T01:CDS | 35.0% | |
| TTGAAGAAGCCAATTCATCT+TGG | + | Chr1:38419987-38420006 | MsG0180002441.01.T01:CDS | 35.0% | |
| ! | ATTGGCTTCTTCAATGAATC+TGG | - | Chr1:38419981-38420000 | None:intergenic | 35.0% |
| ATTGGAAGGATGATAATCCG+CGG | + | Chr1:38420095-38420114 | MsG0180002441.01.T01:CDS | 40.0% | |
| ! | TGAAAATGATGATGCTGCAC+AGG | + | Chr1:38420051-38420070 | MsG0180002441.01.T01:CDS | 40.0% |
| !! | TAGAGCTTTGGATGATTGGA+AGG | + | Chr1:38420081-38420100 | MsG0180002441.01.T01:CDS | 40.0% |
| AATTCGAAGCTTACCCCTTG+TGG | + | Chr1:38420127-38420146 | MsG0180002441.01.T01:CDS | 45.0% | |
| ACAGGATAGAGCTAGAGCTT+TGG | + | Chr1:38420069-38420088 | MsG0180002441.01.T01:CDS | 45.0% | |
| GAAGTTTAAGCCAGGTGAAG+AGG | + | Chr1:38420021-38420040 | MsG0180002441.01.T01:CDS | 45.0% | |
| TTGGAAGGATGATAATCCGC+GGG | + | Chr1:38420096-38420115 | MsG0180002441.01.T01:CDS | 45.0% | |
| !! | GAGCTAGAGCTTTGGATGAT+TGG | + | Chr1:38420077-38420096 | MsG0180002441.01.T01:CDS | 45.0% |
| GTTTAAGCCAGGTGAAGAGG+AGG | + | Chr1:38420024-38420043 | MsG0180002441.01.T01:CDS | 50.0% | |
| TGGAAGGATGATAATCCGCG+GGG | + | Chr1:38420097-38420116 | MsG0180002441.01.T01:CDS | 50.0% | |
| TAAGCCAGGTGAAGAGGAGG+AGG | + | Chr1:38420027-38420046 | MsG0180002441.01.T01:CDS | 55.0% | |
| TCATCCTCCTCCTCTTCACC+TGG | - | Chr1:38420034-38420053 | None:intergenic | 55.0% | |
| TTCGAATTTCCTGCACCCCG+CGG | - | Chr1:38420115-38420134 | None:intergenic | 55.0% | |
| GATGATAATCCGCGGGGTGC+AGG | + | Chr1:38420103-38420122 | MsG0180002441.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 38419914 | 38420153 | 38419914 | ID=MsG0180002441.01;Name=MsG0180002441.01 |
| Chr1 | mRNA | 38419914 | 38420153 | 38419914 | ID=MsG0180002441.01.T01;Parent=MsG0180002441.01;Name=MsG0180002441.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|79 |
| Chr1 | exon | 38419914 | 38420153 | 38419914 | ID=MsG0180002441.01.T01:exon:4506;Parent=MsG0180002441.01.T01 |
| Chr1 | CDS | 38419914 | 38420153 | 38419914 | ID=MsG0180002441.01.T01:cds;Parent=MsG0180002441.01.T01 |
| Gene Sequence |
| Protein sequence |
