AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046058.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046058.01.T01 MTR_4g098710 93.750 64 4 0 1 64 1 64 2.09e-28 97.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAATTCAGAATCTTATC+TGG 0.320719 8:+68403052 None:intergenic
GTGATCAGGATGAACAGTTT+AGG 0.424887 8:-68402982 MsG0880046058.01.T01:CDS
TGTTCATCCTGATCACAGTT+TGG 0.447960 8:+68402989 None:intergenic
TGTGTTGCCAAACTGTGATC+AGG 0.505518 8:-68402996 MsG0880046058.01.T01:CDS
TCACTAGACTCCTTCTTGCT+CGG 0.520514 8:+68403118 None:intergenic
AAGATCGGATCGATTGCAAT+GGG 0.546672 8:-68402918 MsG0880046058.01.T01:CDS
TCTCAGAGCAAGAAATCAGA+TGG 0.548303 8:-68403022 MsG0880046058.01.T01:CDS
CAAGATCGGATCGATTGCAA+TGG 0.580943 8:-68402919 MsG0880046058.01.T01:CDS
GAATCAACTGATGTCAAGAT+CGG 0.588286 8:-68402933 MsG0880046058.01.T01:CDS
CAAGAAGGAGTCTAGTGAAG+TGG 0.591426 8:-68403113 MsG0880046058.01.T01:CDS
TAGCAAGTCACCGAGCAAGA+AGG 0.600583 8:-68403128 MsG0880046058.01.T01:CDS
CTCAGAGCAAGAAATCAGAT+GGG 0.605996 8:-68403021 MsG0880046058.01.T01:CDS
TCAGAGCAAGAAATCAGATG+GGG 0.702066 8:-68403020 MsG0880046058.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTGAATTCAGAATCTTATC+TGG + Chr8:68402982-68403001 None:intergenic 25.0%
GAATCAACTGATGTCAAGAT+CGG - Chr8:68403098-68403117 MsG0880046058.01.T01:CDS 35.0%
AAGATCGGATCGATTGCAAT+GGG - Chr8:68403113-68403132 MsG0880046058.01.T01:CDS 40.0%
CTCAGAGCAAGAAATCAGAT+GGG - Chr8:68403010-68403029 MsG0880046058.01.T01:CDS 40.0%
GTGATCAGGATGAACAGTTT+AGG - Chr8:68403049-68403068 MsG0880046058.01.T01:CDS 40.0%
TCAGAGCAAGAAATCAGATG+GGG - Chr8:68403011-68403030 MsG0880046058.01.T01:CDS 40.0%
TCTCAGAGCAAGAAATCAGA+TGG - Chr8:68403009-68403028 MsG0880046058.01.T01:CDS 40.0%
TGTTCATCCTGATCACAGTT+TGG + Chr8:68403045-68403064 None:intergenic 40.0%
CAAGAAGGAGTCTAGTGAAG+TGG - Chr8:68402918-68402937 MsG0880046058.01.T01:CDS 45.0%
CAAGATCGGATCGATTGCAA+TGG - Chr8:68403112-68403131 MsG0880046058.01.T01:CDS 45.0%
TCACTAGACTCCTTCTTGCT+CGG + Chr8:68402916-68402935 None:intergenic 45.0%
TGTGTTGCCAAACTGTGATC+AGG - Chr8:68403035-68403054 MsG0880046058.01.T01:CDS 45.0%
TAGCAAGTCACCGAGCAAGA+AGG - Chr8:68402903-68402922 MsG0880046058.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 68402898 68403155 68402898 ID=MsG0880046058.01;Name=MsG0880046058.01
Chr8 mRNA 68402898 68403155 68402898 ID=MsG0880046058.01.T01;Parent=MsG0880046058.01;Name=MsG0880046058.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|85
Chr8 exon 68402898 68403155 68402898 ID=MsG0880046058.01.T01:exon:32603;Parent=MsG0880046058.01.T01
Chr8 CDS 68402898 68403155 68402898 ID=MsG0880046058.01.T01:cds;Parent=MsG0880046058.01.T01
Gene Sequence

>MsG0880046058.01.T01

ATGGATAGCAAGTCACCGAGCAAGAAGGAGTCTAGTGAAGTGGCAGCACGCGAGTCGCTTATTGCTATTTCTAACTCTTCACCAGATAAGATTCTGAATTCAAATTCTGTTTCTCAGAGCAAGAAATCAGATGGGGCTGTGTTGCCAAACTGTGATCAGGATGAACAGTTTAGGTCTGAACTGATTTCAATTCGTATGCTGAATCAACTGATGTCAAGATCGGATCGATTGCAATGGGAGTCTAAACTGCAGCTATAA

Protein sequence

>MsG0880046058.01.T01

MDSKSPSKKESSEVAARESLIAISNSSPDKILNSNSVSQSKKSDGAVLPNCDQDEQFRSELISIRMLNQLMSRSDRLQWESKLQL*