Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003232.01.T01 | XP_003590299.1 | 93.846 | 130 | 8 | 0 | 1 | 130 | 624 | 753 | 5.12E-77 | 252 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003232.01.T01 | G7I7Z4 | 93.846 | 130 | 8 | 0 | 1 | 130 | 624 | 753 | 2.45e-77 | 252 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003232.01 | MsG0280007089.01 | 0.817739 | 2.820689e-52 | 2.071249e-49 |
| MsG0180003232.01 | MsG0280007761.01 | 0.812749 | 3.624114e-51 | 2.323253e-48 |
| MsG0180003232.01 | MsG0280010868.01 | 0.827483 | 1.525734e-54 | 1.474922e-51 |
| MsG0180003232.01 | MsG0280011171.01 | 0.808167 | 3.537612e-50 | 2.008436e-47 |
| MsG0180003232.01 | MsG0380015455.01 | 0.800994 | 1.109654e-48 | 5.240744e-46 |
| MsG0180003232.01 | MsG0880046165.01 | 0.810015 | 1.421791e-50 | 8.476532e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003232.01.T01 | MTR_1g056180 | 93.846 | 130 | 8 | 0 | 1 | 130 | 624 | 753 | 6.20e-81 | 252 |
| MsG0180003232.01.T01 | MTR_7g106540 | 50.407 | 123 | 51 | 4 | 6 | 127 | 421 | 534 | 1.67e-28 | 108 |
| MsG0180003232.01.T01 | MTR_7g106540 | 52.101 | 119 | 47 | 4 | 6 | 123 | 618 | 727 | 3.64e-27 | 105 |
| MsG0180003232.01.T01 | MTR_3g107090 | 44.944 | 89 | 46 | 2 | 20 | 105 | 509 | 597 | 1.83e-16 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003232.01.T01 | AT3G11760 | 74.194 | 62 | 15 | 1 | 46 | 106 | 633 | 694 | 3.41e-25 | 99.8 |
| MsG0180003232.01.T01 | AT5G04860 | 40.458 | 131 | 52 | 4 | 20 | 126 | 627 | 755 | 4.17e-22 | 90.9 |
| MsG0180003232.01.T01 | AT5G04860 | 57.692 | 78 | 23 | 2 | 57 | 126 | 529 | 604 | 5.07e-22 | 90.5 |
| MsG0180003232.01.T01 | AT2G10560 | 65.000 | 60 | 19 | 1 | 51 | 110 | 170 | 227 | 8.25e-21 | 85.1 |
| MsG0180003232.01.T01 | AT2G25460 | 50.000 | 64 | 29 | 2 | 51 | 111 | 482 | 545 | 3.47e-12 | 62.4 |
Find 19 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGCTTGTAATTCTCTAAT+AGG | 0.276013 | 1:-58687678 | None:intergenic |
| TTGGAGTCTGGTGCTGAGAC+AGG | 0.452085 | 1:+58687561 | MsG0180003232.01.T01:CDS |
| AATGATAAGGAGTTGGAGTC+TGG | 0.455949 | 1:+58687549 | MsG0180003232.01.T01:CDS |
| TCTGACGATTGAGTGGCATC+TGG | 0.491709 | 1:-58687453 | None:intergenic |
| GCAAATCAATGATAAGGAGT+TGG | 0.514949 | 1:+58687542 | MsG0180003232.01.T01:CDS |
| TGCAACGGTTTGTTGTTCAC+CGG | 0.516703 | 1:-58687487 | None:intergenic |
| TTACAAGCAGATGTGAAGAA+AGG | 0.518971 | 1:+58687690 | MsG0180003232.01.T01:CDS |
| GGTTCAGCAAATCAATGATA+AGG | 0.524830 | 1:+58687536 | MsG0180003232.01.T01:CDS |
| GTCTTGTGATGTTGTTCCTG+TGG | 0.538631 | 1:+58687779 | MsG0180003232.01.T01:CDS |
| TCGTCAGACGAAAACACAAC+CGG | 0.545059 | 1:+58687468 | MsG0180003232.01.T01:CDS |
| ATTTGTAGTCTATGATGAAG+TGG | 0.567209 | 1:-58687729 | None:intergenic |
| ACAGATGCTTCTTCAGCCAC+AGG | 0.578140 | 1:-58687795 | None:intergenic |
| ATGTATTAGAGTACAATGAC+AGG | 0.595845 | 1:+58687511 | MsG0180003232.01.T01:CDS |
| GTACTCTAATACATCTGCAA+CGG | 0.605082 | 1:-58687502 | None:intergenic |
| ATTAGAGTACAATGACAGGC+AGG | 0.605990 | 1:+58687515 | MsG0180003232.01.T01:CDS |
| TCAGTCGAAAAGTGAGCGTG+AGG | 0.606612 | 1:+58687587 | MsG0180003232.01.T01:CDS |
| AAGTGAGCGTGAGGAAGACG+AGG | 0.642594 | 1:+58687596 | MsG0180003232.01.T01:CDS |
| CAGAGGGAAAGAATCATGCA+AGG | 0.651300 | 1:+58687626 | MsG0180003232.01.T01:CDS |
| AGCATCTGTCACAGTAACTG+AGG | 0.656388 | 1:+58687809 | MsG0180003232.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGTATTAGAGTACAATGAC+AGG | + | Chr1:58687511-58687530 | MsG0180003232.01.T01:CDS | 30.0% | |
| ATTTGTAGTCTATGATGAAG+TGG | - | Chr1:58687732-58687751 | None:intergenic | 30.0% | |
| TCTGCTTGTAATTCTCTAAT+AGG | - | Chr1:58687681-58687700 | None:intergenic | 30.0% | |
| GCAAATCAATGATAAGGAGT+TGG | + | Chr1:58687542-58687561 | MsG0180003232.01.T01:CDS | 35.0% | |
| GGTTCAGCAAATCAATGATA+AGG | + | Chr1:58687536-58687555 | MsG0180003232.01.T01:CDS | 35.0% | |
| GTACTCTAATACATCTGCAA+CGG | - | Chr1:58687505-58687524 | None:intergenic | 35.0% | |
| TTACAAGCAGATGTGAAGAA+AGG | + | Chr1:58687690-58687709 | MsG0180003232.01.T01:CDS | 35.0% | |
| AATGATAAGGAGTTGGAGTC+TGG | + | Chr1:58687549-58687568 | MsG0180003232.01.T01:CDS | 40.0% | |
| ATTAGAGTACAATGACAGGC+AGG | + | Chr1:58687515-58687534 | MsG0180003232.01.T01:CDS | 40.0% | |
| AGCATCTGTCACAGTAACTG+AGG | + | Chr1:58687809-58687828 | MsG0180003232.01.T01:CDS | 45.0% | |
| CAGAGGGAAAGAATCATGCA+AGG | + | Chr1:58687626-58687645 | MsG0180003232.01.T01:CDS | 45.0% | |
| GTCTTGTGATGTTGTTCCTG+TGG | + | Chr1:58687779-58687798 | MsG0180003232.01.T01:CDS | 45.0% | |
| TCGTCAGACGAAAACACAAC+CGG | + | Chr1:58687468-58687487 | MsG0180003232.01.T01:CDS | 45.0% | |
| ! | GTTTTCGTCTGACGATTGAG+TGG | - | Chr1:58687463-58687482 | None:intergenic | 45.0% |
| !! | AAGACGAGGTTTTGTGCAGA+GGG | + | Chr1:58687610-58687629 | MsG0180003232.01.T01:CDS | 45.0% |
| !! | TGCAACGGTTTGTTGTTCAC+CGG | - | Chr1:58687490-58687509 | None:intergenic | 45.0% |
| ACAGATGCTTCTTCAGCCAC+AGG | - | Chr1:58687798-58687817 | None:intergenic | 50.0% | |
| TCAGTCGAAAAGTGAGCGTG+AGG | + | Chr1:58687587-58687606 | MsG0180003232.01.T01:CDS | 50.0% | |
| TCTGACGATTGAGTGGCATC+TGG | - | Chr1:58687456-58687475 | None:intergenic | 50.0% | |
| !! | GAAGACGAGGTTTTGTGCAG+AGG | + | Chr1:58687609-58687628 | MsG0180003232.01.T01:CDS | 50.0% |
| AAGTGAGCGTGAGGAAGACG+AGG | + | Chr1:58687596-58687615 | MsG0180003232.01.T01:CDS | 55.0% | |
| ! | TTGGAGTCTGGTGCTGAGAC+AGG | + | Chr1:58687561-58687580 | MsG0180003232.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 58687450 | 58687842 | 58687450 | ID=MsG0180003232.01;Name=MsG0180003232.01 |
| Chr1 | mRNA | 58687450 | 58687842 | 58687450 | ID=MsG0180003232.01.T01;Parent=MsG0180003232.01;Name=MsG0180003232.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|130 |
| Chr1 | exon | 58687450 | 58687842 | 58687450 | ID=MsG0180003232.01.T01:exon:26939;Parent=MsG0180003232.01.T01 |
| Chr1 | CDS | 58687450 | 58687842 | 58687450 | ID=MsG0180003232.01.T01:cds;Parent=MsG0180003232.01.T01 |
| Gene Sequence |
| Protein sequence |
